https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbs-rabbit-monoclonal-antibody-m00130-1-boster.html2026-03-17T05:11:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00130-1-wb.jpgAnti-CBS Rabbit Monoclonal AntibodyWestern blot analysis of CBS expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bcl10-rabbit-monoclonal-antibody-m01616-4-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01616-4-wb.jpgAnti-Bcl10 Rabbit Monoclonal AntibodyWestern blot analysis of Bcl10 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-guanylyl-cyclase-beta-1-rabbit-monoclonal-antibody-m32396-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32396-gucy1b1-primary-antibodies-wb-testing-1_1.jpgAnti-Guanylyl Cyclase beta 1 Rabbit Monoclonal AntibodyWestern blot analysis of GUCY1B1 using anti-GUCY1B1 antibody (M32396). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GUCY1B1 antigen affinity purified monoclonal antibody (M32396) at 1:500 overnight at 4°C, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GUCY1B1 at approximately 71 kDa. The expected band size for GUCY1B1 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32396-gucy1b1-primary-antibodies-ihc-testing-1_1.jpgAnti-Guanylyl Cyclase beta 1 Rabbit Monoclonal AntibodyIHC analysis of GUCY1B1 using anti-GUCY1B1 antibody (M32396). GUCY1B1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GUCY1B1 Antibody (M32396) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32396-gucy1b1-primary-antibodies-ihc-testing-2_1.jpgAnti-Guanylyl Cyclase beta 1 Rabbit Monoclonal AntibodyIHC analysis of GUCY1B1 using anti-GUCY1B1 antibody (M32396). GUCY1B1 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GUCY1B1 Antibody (M32396) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32396-gucy1b1-primary-antibodies-ihc-testing-3_1.jpgAnti-Guanylyl Cyclase beta 1 Rabbit Monoclonal AntibodyIHC analysis of GUCY1B1 using anti-GUCY1B1 antibody (M32396). GUCY1B1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GUCY1B1 Antibody (M32396) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32396-gucy1b1-primary-antibodies-ihc-testing-4_1.jpgAnti-Guanylyl Cyclase beta 1 Rabbit Monoclonal AntibodyIHC analysis of GUCY1B1 using anti-GUCY1B1 antibody (M32396). GUCY1B1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GUCY1B1 Antibody (M32396) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32396-gucy1b1-primary-antibodies-ihc-testing-5_1.jpgAnti-Guanylyl Cyclase beta 1 Rabbit Monoclonal AntibodyIHC analysis of GUCY1B1 using anti-GUCY1B1 antibody (M32396). GUCY1B1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GUCY1B1 Antibody (M32396) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cxcr5-rabbit-monoclonal-antibody-m00663-1-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00663-1-cxcr5-primary-antibodies-wb-testing-1.jpgAnti-CXCR5 Rabbit Monoclonal Antibody Western blot analysis of CXCR5 using anti-CXCR5 antibody (M00663-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR5 antigen affinity purified monoclonal antibody (M00663-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR5 at approximately 42 kDa. The expected band size for CXCR5 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00663-1-cxcr5-primary-antibodies-ihc-testing-2.jpgAnti-CXCR5 Rabbit Monoclonal Antibody IHC analysis of CXCR5 using anti-CXCR5 antibody (M00663-1). CXCR5 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CXCR5 Antibody (M00663-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-baf57-smarce1-rabbit-monoclonal-antibody-m03739-1-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03739-1-wb.jpgAnti-BAF57/SMARCE1 Rabbit Monoclonal AntibodyWestern blot analysis of BAF57/SMARCE1 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lumican-rabbit-monoclonal-antibody-m01034-1-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01034-1-lum-primary-antibodies-wb-testing-1.jpgAnti-Lumican Rabbit Monoclonal AntibodyWestern blot analysis of Lumican expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01034-1-lumican-primary-antibodies-if-testing-2.jpgAnti-Lumican Rabbit Monoclonal Antibody IF analysis of Lumican using anti-Lumican antibody (M01034-1). Lumican was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-Lumican Antibody (M01034-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01034-1-wb7.jpgAnti-Lumican Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01034-1-wb8.jpgAnti-Lumican Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01034-1-wb9.jpgAnti-Lumican Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmd4-rabbit-monoclonal-antibody-m03544-1-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03544-1-wb.jpgAnti-PSMD4 Rabbit Monoclonal AntibodyWestern blot analysis of Proteasome 19S S5A expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mammaglobin-a-rabbit-monoclonal-antibody-m09363-2-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09363-2-wb.jpgAnti-Mammaglobin A Rabbit Monoclonal AntibodyWestern blot analysis of Mammaglobin A expression in Human breast cancer lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-limk1-rabbit-monoclonal-antibody-m01323-1-boster.html2026-03-17T05:11:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01323-1-wb.jpgAnti-LIMK1 Rabbit Monoclonal AntibodyWestern blot analysis of LIM Kinase 1 expression in U-87MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fbx32-rabbit-monoclonal-antibody-m02531-1-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02531-1-fbx32-primary-antibodies-wb-testing-1_1.jpgAnti-Fbx32 Rabbit Monoclonal AntibodyWestern blot analysis of FBX32 using anti-FBX32 antibody (M02531-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBX32 antigen affinity purified monoclonal antibody (M02531-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBX32 at approximately 42 kDa. The expected band size for FBX32 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02531-1-fbxo32-primary-antibodies-wb-testing-2.jpgAnti-Fbx32 Rabbit Monoclonal AntibodyWestern blot analysis of FBX32 using anti-FBX32 antibody (M02531-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBX32 antigen affinity purified monoclonal antibody (M02531-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBX32 at approximately 42 kDa. The expected band size for FBX32 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pen2-rabbit-monoclonal-antibody-m04504-1-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04504-1-pen2-primary-antibodies-wb-testing-1.jpgAnti-PEN2 Rabbit Monoclonal Antibody Western blot analysis of PEN2 using anti-PEN2 antibody (M04504-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEN2 antigen affinity purified monoclonal antibody (Catalog # M04504-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEN2 at approximately 12 kDa. The expected band size for PEN2 is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04504-1-pen2-primary-antibodies-wb-testing-2.jpgAnti-PEN2 Rabbit Monoclonal AntibodyWestern blot analysis of PEN2 using anti-PEN2 antibody (M04504-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: Rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEN2 antigen affinity purified monoclonal antibody (M04504-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PEN2 at approximately 12 kDa. The expected band size for PEN2 is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldh1a2-rabbit-monoclonal-antibody-m04961-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04961-wb.jpgAnti-ALDH1A2 Rabbit Monoclonal AntibodyWestern blot analysis of ALDH1A2 expression in Molt4 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sfrp4-rabbit-monoclonal-antibody-m03147-1-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03147-1-sfrp4-primary-antibodies-wb-testing-1.jpgAnti-SFRP4 Rabbit Monoclonal Antibody Western blot analysis of SFRP4 using anti-SFRP4 antibody (M03147-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP4 antigen affinity purified monoclonal antibody (Catalog # M03147-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP4 at approximately 50 kDa. The expected band size for SFRP4 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyp24a1-rabbit-monoclonal-antibody-m00343-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00343-wb.jpgAnti-CYP24A1 Rabbit Monoclonal AntibodyWestern blot analysis of CYP24A1 expression in Human fetal liver lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eef1a1-rabbit-monoclonal-antibody-m02141-1-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02141-1-wb.jpgAnti-eEF1A1 Rabbit Monoclonal AntibodyWestern blot analysis of eEF1A1 expression in (1) MCF7 cell lysate; (2) Mouse kidney lysate; (2) Rat spleen lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-h-cadherin-rabbit-monoclonal-antibody-m01986-1-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01986-1-ihc7.jpgAnti-H Cadherin Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human cervical cancer, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01986-1-wb.jpgAnti-H Cadherin Rabbit Monoclonal AntibodyWestern blot analysis of H Cadherin expression in Human fetal heart lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01986-1-ihc8.jpgAnti-H Cadherin Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma , using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01986-1-ihc9.jpgAnti-H Cadherin Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Mouse skeletal muscle, using the Antibody at 1:100 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cardiac-troponin-c-rabbit-monoclonal-antibody-m03153-1-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03153-1-wb7.jpgAnti-Cardiac Troponin C Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03153-1-wb.jpgAnti-Cardiac Troponin C Rabbit Monoclonal AntibodyWestern blot analysis of Cardiac Troponin C expression in Human fetal heart lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-integrin-alpha-9-rabbit-monoclonal-antibody-m06851-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06851-wb.jpgAnti-Integrin alpha 9 Rabbit Monoclonal AntibodyWestern blot analysis of Integrin alpha 9 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddb2-rabbit-monoclonal-antibody-m01430-1-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01430-1-wb.jpgAnti-DDB2 Rabbit Monoclonal AntibodyWestern blot analysis of DDB2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mad2l1-rabbit-monoclonal-antibody-m00785-2-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00785-2-wb.jpgAnti-Mad2L1 Rabbit Monoclonal AntibodyWestern blot analysis of Mad2L1 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-khsrp-rabbit-monoclonal-antibody-m02770-2-boster.html2026-03-17T05:11:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02770-2-wb.jpgAnti-KHSRP Rabbit Monoclonal AntibodyWestern blot analysis of KHSRP expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mct1-rabbit-monoclonal-antibody-m02240-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02240-mct1-primary-antibodies-wb-testing-1.jpgAnti-MCT1 Rabbit Monoclonal AntibodyWestern blot analysis of MCT1 using anti-MCT1 antibody (M02240). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCT1 antigen affinity purified monoclonal antibody (M02240) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCT1 at approximately 50 kDa. The expected band size for MCT1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02240-mct1-primary-antibodies-ihc-testing-3.jpgAnti-MCT1 Rabbit Monoclonal AntibodyIHC analysis of MCT1 using anti-MCT1 antibody (M02240). MCT1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MCT1 Antibody (M02240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02240-mct1-primary-antibodies-ihc-testing-2.jpgAnti-MCT1 Rabbit Monoclonal AntibodyIHC analysis of MCT1 using anti-MCT1 antibody (M02240). MCT1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MCT1 Antibody (M02240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tbx21-rabbit-monoclonal-antibody-m00404-1-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00404-1-ihc.jpgAnti-TBX21 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human spleen, using T-bet/Tbx21 Antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-6-rabbit-monoclonal-antibody-m04012-1-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04012-1-wb.jpgAnti-Cytokeratin 6 Rabbit Monoclonal AntibodyWestern blot analysis of CK6 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-satb2-rabbit-monoclonal-antibody-m02588-2-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02588-2-satb2-primary-antibodies-wb-testing-1.jpgAnti-SATB2 Rabbit Monoclonal AntibodyWestern blot analysis of SATB2 using anti-SATB2 antibody (M02588-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB2 antigen affinity purified monoclonal antibody (M02588-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SATB2 at approximately 90 kDa. The expected band size for SATB2 is at 90 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pancreatic-lipase-pnlip-rabbit-monoclonal-antibody-m04935-1-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04935-1-wb.jpgAnti-Pancreatic Lipase （PNLIP） Rabbit Monoclonal AntibodyWestern blot analysis of Pancreatic Lipase (PNLIP) expression in Human pancreas lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myod-rabbit-monoclonal-antibody-m00964-3-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00964-3-myod-primary-antibodies-wb-testing-1.jpgAnti-MyoD Rabbit Monoclonal AntibodyWestern blot analysis of MYOD using anti-MYOD antibody (M00964-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYOD antigen affinity purified monoclonal antibody (M00964-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYOD at approximately 35 kDa. The expected band size for MYOD is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thyroglobulin-rabbit-monoclonal-antibody-m00359-5-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00359-5-wb.jpgAnti-Thyroglobulin Rabbit Monoclonal AntibodyWestern blot analysis of Thyroglobulin expression in Human thyroid lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nm23-rabbit-monoclonal-antibody-m01334-2-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01334-2-wb.jpgAnti-NM23 Rabbit Monoclonal AntibodyWestern blot analysis of NM23 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd105-rabbit-monoclonal-antibody-m02997-5-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02997-5-wb7.jpgAnti-CD105 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02997-5-wb.jpgAnti-CD105 Rabbit Monoclonal AntibodyWestern blot analysis of CD105 expression in HUVEC cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alk-rabbit-monoclonal-antibody-m00301-3-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00301-3-ihc.jpgAnti-ALK Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human kidney, using ALK Antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grb7-rabbit-monoclonal-antibody-m02528-1-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02528-1-wb.jpgAnti-GRB7 Rabbit Monoclonal AntibodyWestern blot analysis of GRB7 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nkx2-2-rabbit-monoclonal-antibody-m04740-1-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04740-1-ihc.jpgAnti-NKX2.2 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human pancreas, using NKX2.2 Antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-egfr-l858r-mutation-rabbit-monoclonal-antibody-m00023-5-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-5-wb.jpgAnti-EGFR(L858R mutation) Rabbit Monoclonal AntibodyWestern blot analysis of EGFR(L858R) expression in (1) HeLa cell lysate; (2) H1975 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clasp1-rabbit-monoclonal-antibody-m04813-1-boster.html2026-03-17T05:11:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04813-1-wb.jpgAnti-CLASP1 Rabbit Monoclonal AntibodyWestern blot analysis of CLASP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-srp54-rabbit-monoclonal-antibody-m06189-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06189-1-wb.jpgAnti-SRP54 Rabbit Monoclonal AntibodyWestern blot analysis of SRP54 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kininogen-1-rabbit-monoclonal-antibody-m02939-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02939-1-wb.jpgAnti-Kininogen 1 Rabbit Monoclonal AntibodyWestern blot analysis of Kininogen 1 expression in Human platelet lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-brachyury-rabbit-monoclonal-antibody-m31984-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31984-1-wb.jpgAnti-Brachyury Rabbit Monoclonal AntibodyWestern blot analysis of Brachyury expression in MUG-Chor1 (human sacral bone chordoma) cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fyb-rabbit-monoclonal-antibody-m32410-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32410-1-fyb-primary-antibodies-wb-testing-1.jpgAnti-FYB Rabbit Monoclonal Antibody Western blot analysis of FYB using anti-FYB antibody (M32410-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human TF-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FYB antigen affinity purified monoclonal antibody (Catalog # M32410-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FYB at approximately 120 kDa. The expected band size for FYB is at 85 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trefoil-factor-3-rabbit-monoclonal-antibody-m01738-2-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01738-2-wb.jpgAnti-Trefoil Factor 3 Rabbit Monoclonal AntibodyWestern blot analysis of Trefoil Factor 3 expression in Human colon cancer lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufs4-rabbit-monoclonal-antibody-m03608-2-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03608-2-ndufs4-primary-antibodies-wb-testing-1_1.jpgAnti-NDUFS4 Rabbit Monoclonal AntibodyWestern blot analysis of NDUFS4 using anti-NDUFS4 antibody (M03608-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS4 antigen affinity purified monoclonal antibody (M03608-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDUFS4 at approximately 18 kDa. The expected band size for NDUFS4 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03608-2-ndufs4-primary-antibodies-ihc-testing-1.jpgAnti-NDUFS4 Rabbit Monoclonal AntibodyIHC analysis of NDUFS4 using anti-NDUFS4 antibody (M03608-2). NDUFS4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFS4 Antibody (M03608-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03608-2-ndufs4-primary-antibodies-ihc-testing-2_1.jpgAnti-NDUFS4 Rabbit Monoclonal AntibodyIHC analysis of NDUFS4 using anti-NDUFS4 antibody (M03608-2). NDUFS4 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFS4 Antibody (M03608-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03608-2-ndufs4-primary-antibodies-ihc-testing-3_1.jpgAnti-NDUFS4 Rabbit Monoclonal AntibodyIHC analysis of NDUFS4 using anti-NDUFS4 antibody (M03608-2). NDUFS4 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFS4 Antibody (M03608-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03608-2-ndufs4-primary-antibodies-ihc-testing-4_1.jpgAnti-NDUFS4 Rabbit Monoclonal AntibodyIHC analysis of NDUFS4 using anti-NDUFS4 antibody (M03608-2). NDUFS4 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFS4 Antibody (M03608-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mark3-rabbit-monoclonal-antibody-m05355-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05355-1-wb.jpgAnti-MARK3 Rabbit Monoclonal AntibodyWestern blot analysis of MARK3 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-protein-phosphatase-1-beta-rabbit-monoclonal-antibody-m04022-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04022-1-wb.jpgAnti-Protein Phosphatase 1 beta Rabbit Monoclonal AntibodyWestern blot analysis of Protein Phosphatase 1 beta expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rfa2-rabbit-monoclonal-antibody-m02067-3-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-3-rpa2-primary-antibodies-wb-testing-1.jpgAnti-RFA2 Rabbit Monoclonal AntibodyWestern blot analysis of RPA2 using anti-RPA2 antibody (M02067-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA2 antigen affinity purified monoclonal antibody (M02067-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPA2 at approximately 30 kDa. The expected band size for RPA2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-3-rpa2-primary-antibodies-ihc-testing-1.jpgAnti-RFA2 Rabbit Monoclonal AntibodyIHC analysis of RPA2 using anti-RPA2 antibody (M02067-3). RPA2 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPA2 Antibody (M02067-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-3-rpa2-primary-antibodies-ihc-testing-2.jpgAnti-RFA2 Rabbit Monoclonal AntibodyIHC analysis of RPA2 using anti-RPA2 antibody (M02067-3). RPA2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPA2 Antibody (M02067-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-3-rpa2-primary-antibodies-ihc-testing-3.jpgAnti-RFA2 Rabbit Monoclonal AntibodyIHC analysis of RPA2 using anti-RPA2 antibody (M02067-3). RPA2 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPA2 Antibody (M02067-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-3-rpa2-primary-antibodies-ihc-testing-4.jpgAnti-RFA2 Rabbit Monoclonal AntibodyIHC analysis of RPA2 using anti-RPA2 antibody (M02067-3). RPA2 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPA2 Antibody (M02067-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-actinin-2-rabbit-monoclonal-antibody-m03673-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03673-1-wb.jpgAnti-Alpha actinin 2 Rabbit Monoclonal AntibodyWestern blot analysis of Alpha actinin 2 expression in human skeletal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp2a1-serca1-rabbit-monoclonal-antibody-m04657-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04657-1-wb.jpgAnti-ATP2A1/SERCA1 Rabbit Monoclonal AntibodyWestern blot analysis of ATP2A1/SERCA1 expression in human fetal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vav1-2-3-rabbit-monoclonal-antibody-m00691-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00691-1-vav1-2-3-primary-antibodies-wb-testing-1_1.jpgAnti-Vav1/2/3 Rabbit Monoclonal AntibodyWestern blot analysis of VAV1/2/3 using anti-VAV1/2/3 antibody (M00691-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAV1/2/3 antigen affinity purified monoclonal antibody (M00691-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VAV1/2/3 at approximately 98 kDa. The expected band size for VAV1/2/3 is at 98 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aminopeptidase-a-cd249-rabbit-monoclonal-antibody-m06961-2-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06961-2-wb.jpgAnti-Aminopeptidase A / CD249 Rabbit Monoclonal AntibodyWestern blot analysis of Aminopeptidase A / CD249 expression in human fetal lung lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf40-rabbit-monoclonal-antibody-m06979-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06979-1-wb.jpgAnti-RNF40 Rabbit Monoclonal AntibodyWestern blot analysis of RNF40 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-srsf3-rabbit-monoclonal-antibody-m03127-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03127-1-wb7.jpgAnti-SRSF3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03127-1-wb8.jpgAnti-SRSF3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03127-1-wb.jpgAnti-SRSF3 Rabbit Monoclonal AntibodyWestern blot analysis of SRSF3 expression in (1) Jurkat cell lysate; (2) Rat brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mettl3-rabbit-monoclonal-antibody-m01758-1-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01758-1-mettl3-primary-antibodies-wb-testing-1.jpgAnti-METTL3 Rabbit Monoclonal Antibody Western blot analysis of METTL3 using anti-METTL3 antibody (M01758-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified monoclonal antibody (Catalog # M01758-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL3 at approximately 70 kDa. The expected band size for METTL3 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atad2-rabbit-monoclonal-antibody-m03855-boster.html2026-03-17T05:11:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-wb7.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-wb.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyWestern blot analysis of ATAD2 expression in MCF-7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-1-s2.0-s2352304225002995-gr3.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyThe expression of the druggable target ATAD2 in glioma. (A) Visualization of DepMap database-based druggability analysis of CTARS-related proteins using a network Venn diagram. (B) Analysis using the GEPIA database analysis revealed significant upregulation of ATAD2 mRNA expression in glioblastoma (GBM). (C) The UALCAN database analysis showed significant up-regulation of ATAD2 protein in GBM. (D) The expression of ATAD2 mRNA across different clinicopathological characteristics was analyzed in the CGGA cohort. Statistical analyses utilized the Kruskal–Wallis H test followed by post hoc Dunn's test for WHO grade comparisons and the Wilcoxon rank-sum test for other parameters. (E) Representative cases showing immunohistochemical staining across various clinicopathological tissue types of gliomas. Scale bar: 100 μm. (F) Statistical analysis of the IHC scores based on the staining intensity and the percentage of positive cells. Statistical analysis was performed with the Kruskal–Wallis H test and by post hoc Dunn's test. Significance levels are indicated as follows: ns, not significant; ∗, P < 0.05; ∗∗∗∗, P < 0.0001. Index in Genes & Diseases under a CC BY license. DOI: <a href='https://www.sciencedirect.com/science/article/pii/S2352304225002995'>10.1016/j.gendis.2025.101810</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-1-s2.0-s2352304225002995-gr4.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyThe impact of ATAD2 on the malignant phenotype of glioma cells. (A) The Western blot analysis shows the differential expression of ATAD2 in a normal astrocyte cell line (HA1800) and various glioma cell lines. (B) The Western blot analysis demonstrates the efficacy of three ATAD2 shRNAs in reducing ATAD2 expression in the LN229 and U251MG cell lines. (C) The Western blot analysis revealed the expression levels of ATAD2 and the FLAG-tagged protein following ATAD2 overexpression in U118MG cells. (D–F) The CCK-8 assay shows changes in cell proliferation after knockdown of ATAD2 in LN229 and U251MG cells, and its overexpression in U118MG cells. (G, H) Colony formation assay and statistical analysis. (I, J) Migration and invasion assays and their statistical analysis. The data are presented as means ± SD. For panels (D–F), two-way ANOVA was performed, while unpaired two-tailed Student's t-tests were used for panels (H, I). Statistical significance is denoted as follows: ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. Index in Genes & Diseases under a CC BY license. DOI: <a href='https://www.sciencedirect.com/science/article/pii/S2352304225002995'>10.1016/j.gendis.2025.101810</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-1-s2.0-s2352304225002995-gr5.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyThe impact of ATAD2 knockdown on Glioma tumorigenesis in vivo. (A) Gross morphological observations were conducted on subcutaneous tumors induced by LN229 cells in the Shco and Sh-3 groups of nude mice. (B) Statistical analysis of the subcutaneous tumor size measurements. (C) Statistical analysis of the subcutaneous xenograft tumor weights. (D) Representative images of IHC staining for ATAD2 and Ki67. Scale bar:100 μm. (E, F) The Image-Pro Plus software (version 6) was used to compute the sum integrated optical density of ATAD2 and Ki-67 IHC staining, followed by statistical analysis. (G) Representative HE staining images of intracranial orthotopic xenografts formed by LN229 cells in the Shco and Sh-3 groups. Scale bar: 1 mm. (H) Statistical analysis of intracranial orthotopic tumor size measurements. (I) Kaplan–Meier survival curve of nude mice with orthotopic intracranial tumors (Log-rank test). The data are presented as means ± SD, two-tailed paired Student's t-test was used to compare the results in (B), (C), (E), and (F); and two-tailed unpaired Student's t-test was used to compare the results in (H). Each group included n = 6 mice. Statistical significance is denoted as follows: ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. Index in Genes & Diseases under a CC BY license. DOI: <a href='https://www.sciencedirect.com/science/article/pii/S2352304225002995'>10.1016/j.gendis.2025.101810</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-1-s2.0-s2352304225002995-gr6.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyATAD2-E2F1 positive feedback loop regulates the expression of PDK1. (A) Volcano plot of differentially expressed genes from RNA-seq analysis of ATAD2 knockdown and control conditions in LN229 cells. (B) Volcano plot of differentially expressed proteins from proteome analysis of ATAD2 knockdown and control conditions in LN229 cells. (C) The Venn diagram illustrates the overlap between the up-regulated and down-regulated mRNAs and proteins. (D) The fold change ranking plot illustrates 20 commonly down-regulated proteins. (E, F) Western blot analysis confirmed that ATAD2 enhances the expression of PDK1. (G, H) Western blot analysis confirmed that E2F1 up-regulates the expression of ATAD2. (I–K) Western blot analysis validated that E2F1 enhances the expression of ATAD2. (L, M) Western blot analysis showed that ATAD2 cooperates with E2F1 to up-regulate the expression of PDK1. (N) The dual luciferase reporter gene assays revealed that ATAD2 and E2F1 synergistically enhance the promoter activity of PDK1. The data are presented as means ± SD. Statistical comparisons were performed using unpaired two-tailed Student's t-test for figures (F), (H), and (K), and one-way ANOVA followed by Tukey's post hoc test for figures (J), (M), and (N). Statistical significance is denoted as follows: ns, not significant; ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. Index in Genes & Diseases under a CC BY license. DOI: <a href='https://www.sciencedirect.com/science/article/pii/S2352304225002995'>10.1016/j.gendis.2025.101810</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-1-s2.0-s2352304225002995-gr7.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyThe clinical significance of the ATAD2-E2F1-PDK1 axis in glioma. (A, B) Analysis using the GlioVis database showed a positive correlation between the expressions of ATAD2, E2F1, and PDK1, as determined by the Spearman correlation test. (C) Representative cases showed the expression of E2F1 and PDK1 in glioma clinical specimens with low and high expression of ATAD2. Scale bar: 100 μm. (D) Spearman's correlation analysis of ATAD2, E2F1, and PDK1 expression levels in glioma clinical specimens. (E) Kaplan–Meier analysis and log-rank test of survival rates across distinct co-expression groups of ATAD2, E2F1, and PDK1 in the CGGA cohort. P values were adjusted for multiple group comparisons using the Bonferroni method. (F) Graphical diagram illustrating that ATAD2 promotes glioma progression and synergizes with E2F1 to increase PDK1 expression. The figure was created using Figdraw (www.figdraw.com). Statistical significance is denoted as follows: ∗∗∗, P < 0.001. Index in Genes & Diseases under a CC BY license. DOI: <a href='https://www.sciencedirect.com/science/article/pii/S2352304225002995'>10.1016/j.gendis.2025.101810</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-icc1.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03855-icc2.jpgAnti-ATAD2 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hb-egf-rabbit-monoclonal-antibody-m01759-1-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01759-1-hbegf-primary-antibodies-wb-testing-1.jpgAnti-HB EGF Rabbit Monoclonal Antibody Western blot analysis of HBEGF using anti-HBEGF antibody (M01759-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBEGF antigen affinity purified monoclonal antibody (Catalog # M01759-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HBEGF at approximately 23 kDa. The expected band size for HBEGF is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-golph4-rabbit-monoclonal-antibody-m10120-1-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10120-1-wb.jpgAnti-GOLPH4 Rabbit Monoclonal AntibodyWestern blot analysis of GOLPH4 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cenexin1-odf2-rabbit-monoclonal-antibody-m05599-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05599-wb.jpgAnti-Cenexin1 / ODF2 Rabbit Monoclonal AntibodyWestern blot analysis of Cenexin1/ODF2 expression in Hela cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05599-wb7.jpgAnti-Cenexin1 / ODF2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trpc1-rabbit-monoclonal-antibody-m01492-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01492-trpc1-primary-antibodies-wb-testing-1.jpgAnti-TRPC1 Rabbit Monoclonal AntibodyWestern blot analysis of TRPC1 using anti-TRPC1 antibody (M01492). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC1 antigen affinity purified monoclonal antibody (M01492) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRPC1 at approximately 120 kDa. The expected band size for TRPC1 is at 91 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sec61a-rabbit-monoclonal-antibody-m17281-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m17281-wb.jpgAnti-SEC61A Rabbit Monoclonal AntibodyWestern blot analysis of SEC61A expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tnni2-rabbit-monoclonal-antibody-m07355-1-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07355-1-wb.jpgAnti-TNNI2 Rabbit Monoclonal AntibodyWestern blot analysis of Troponin I fast skeletal muscle expression in Human skeletal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pold1-rabbit-monoclonal-antibody-m03720-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03720-wb7.jpgAnti-POLD1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03720-wb8.jpgAnti-POLD1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03720-wb.jpgAnti-POLD1 Rabbit Monoclonal AntibodyWestern blot analysis of DNA Polymerase delta, catalytic subunit expression in (1) Jurkat cell lysate; (2) Raw264.7 cell lysate; (3) C6 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03720-pold1-primary-antibodies-ihc-testing-4.jpgAnti-POLD1 Rabbit Monoclonal Antibody IHC analysis of POLD1 using anti-POLD1 antibody (M03720) . POLD1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03720-pold1-primary-antibodies-ihc-testing-5.jpgAnti-POLD1 Rabbit Monoclonal Antibody IHC analysis of POLD1 using anti-POLD1 antibody (M03720) . POLD1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03720-pold1-primary-antibodies-ihc-testing-6.jpgAnti-POLD1 Rabbit Monoclonal Antibody IHC analysis of POLD1 using anti-POLD1 antibody (M03720) . POLD1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03720-pold1-primary-antibodies-ihc-testing-7.jpgAnti-POLD1 Rabbit Monoclonal Antibody IHC analysis of POLD1 using anti-POLD1 antibody (M03720) . POLD1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLD1 Antibody (M03720) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-syntaxin-rabbit-monoclonal-antibody-m06932-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06932-wb7.jpgAnti-Syntaxin Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06932-wb.jpgAnti-Syntaxin Rabbit Monoclonal AntibodyWestern blot analysis of Syntaxin expression in Human fetal brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-syntaxin-4-rabbit-monoclonal-antibody-m05345-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05345-wb7.jpgAnti-Syntaxin 4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05345-wb8.jpgAnti-Syntaxin 4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05345-wb.jpgAnti-Syntaxin 4 Rabbit Monoclonal AntibodyWestern blot analysis of Syntaxin 4 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dkk3-rabbit-monoclonal-antibody-m02248-1-boster.html2026-03-17T05:11:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02248-1-dkk3-primary-antibodies-wb-testing-1.jpgAnti-Dkk3 Rabbit Monoclonal AntibodyWestern blot analysis of DKK3 using anti-DKK3 antibody (M02248-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK3 antigen affinity purified monoclonal antibody (M02248-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DKK3 at approximately 50 kDa. The expected band size for DKK3 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02248-1-dkk3-primary-antibodies-ihc-testing-1.jpgAnti-Dkk3 Rabbit Monoclonal AntibodyIHC analysis of DKK3 using anti-DKK3 antibody (M02248-1). DKK3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DKK3 Antibody (M02248-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02248-1-dkk3-primary-antibodies-ihc-testing-2.jpgAnti-Dkk3 Rabbit Monoclonal AntibodyIHC analysis of DKK3 using anti-DKK3 antibody (M02248-1). DKK3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DKK3 Antibody (M02248-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02248-1-dkk3-primary-antibodies-ihc-testing-3.jpgAnti-Dkk3 Rabbit Monoclonal AntibodyIHC analysis of DKK3 using anti-DKK3 antibody (M02248-1). DKK3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DKK3 Antibody (M02248-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02248-1-dkk3-primary-antibodies-ihc-testing-4.jpgAnti-Dkk3 Rabbit Monoclonal AntibodyIHC analysis of DKK3 using anti-DKK3 antibody (M02248-1). DKK3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DKK3 Antibody (M02248-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ldha-rabbit-monoclonal-antibody-m00825-1-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-1-ldha-primary-antibodies-wb-testing-1.jpgAnti-LDHA Rabbit Monoclonal Antibody Western blot analysis of LDHA using anti-LDHA antibody (M00825-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HUH-7- WT whole cell lysates, Lane 2: human HUH-7-LDHA KO whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHA antigen affinity purified monoclonal antibody (M00825-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LDHA at approximately 37 kDa. The expected band size for LDHA is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-1-wb10.jpgAnti-LDHA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-1-wb7.jpgAnti-LDHA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-intra-acrosomal-protein-rabbit-monoclonal-antibody-m11311-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11311-wb.jpgAnti-Intra Acrosomal Protein Rabbit Monoclonal AntibodyWestern blot analysis of Intra Acrosomal Protein expression in Human testis lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-akr1c3-rabbit-monoclonal-antibody-m01820-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01820-akr1c3-primary-antibodies-wb-testing-1.jpgAnti-AKR1C3 Rabbit Monoclonal AntibodyWestern blot analysis of AKR1C3 using anti-AKR1C3 antibody (M01820). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C3 antigen affinity purified monoclonal antibody (M01820) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AKR1C3 at approximately 37 kDa. The expected band size for AKR1C3 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-u2af65-rabbit-monoclonal-antibody-m03639-2-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03639-2-wb7.jpgAnti-U2AF65 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03639-2-wb8.jpgAnti-U2AF65 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03639-2-wb.jpgAnti-U2AF65 Rabbit Monoclonal AntibodyWestern blot analysis of U2AF65 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ka1-rabbit-monoclonal-antibody-m09195-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09195-grik4-primary-antibodies-wb-testing-1_1.jpgAnti-KA1 Rabbit Monoclonal Antibody Western blot analysis of GRIK4 using anti-GRIK4 antibody (M09195). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK4 antigen affinity purified monoclonal antibody (M09195) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRIK4 at approximately 107 kDa. The expected band size for GRIK4 is at 107 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09195-wb.jpgAnti-KA1 Rabbit Monoclonal AntibodyWestern blot analysis of KA1 expression in Human hippocampus lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prmt6-rabbit-monoclonal-antibody-m02924-2-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02924-2-prmt6-primary-antibodies-wb-testing-1.jpgAnti-PRMT6 Rabbit Monoclonal AntibodyWestern blot analysis of PRMT6 using anti-PRMT6 antibody (M02924-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT6 antigen affinity purified monoclonal antibody (M02924-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRMT6 at approximately 42 kDa. The expected band size for PRMT6 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kat1-hat1-rabbit-monoclonal-antibody-m03596-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03596-kat1-primary-antibodies-wb-testing-1.jpgAnti-KAT1 / HAT1 Rabbit Monoclonal AntibodyWestern blot analysis of KAT1 using anti-KAT1 antibody (M03596). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT1 antigen affinity purified monoclonal antibody (M03596) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KAT1 at approximately 45 kDa. The expected band size for KAT1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03596-kat1-primary-antibodies-ihc-testing-1.jpgAnti-KAT1 / HAT1 Rabbit Monoclonal AntibodyIHC analysis of KAT1 using anti-KAT1 antibody (M03596). KAT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT1 Antibody (M03596) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03596-kat1-primary-antibodies-ihc-testing-2.jpgAnti-KAT1 / HAT1 Rabbit Monoclonal AntibodyIHC analysis of KAT1 using anti-KAT1 antibody (M03596). KAT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT1 Antibody (M03596) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03596-kat1-primary-antibodies-ihc-testing-3.jpgAnti-KAT1 / HAT1 Rabbit Monoclonal AntibodyIHC analysis of KAT1 using anti-KAT1 antibody (M03596). KAT1 was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT1 Antibody (M03596) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03596-kat1-primary-antibodies-ihc-testing-4.jpgAnti-KAT1 / HAT1 Rabbit Monoclonal AntibodyIHC analysis of KAT1 using anti-KAT1 antibody (M03596). KAT1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KAT1 Antibody (M03596) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp22-rabbit-monoclonal-antibody-m03887-1-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03887-1-usp22-primary-antibodies-wb-testing-1.jpgAnti-USP22 Rabbit Monoclonal AntibodyWestern blot analysis of USP22 using anti-USP22 antibody (M03887-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP22 antigen affinity purified monoclonal antibody (M03887-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for USP22 at approximately 60 kDa. The expected band size for USP22 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03887-1-usp22-primary-antibodies-ihc-testing-1.jpgAnti-USP22 Rabbit Monoclonal AntibodyIHC analysis of USP22 using anti-USP22 antibody (M03887-1). USP22 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-USP22 Antibody (M03887-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03887-1-usp22-primary-antibodies-ihc-testing-2.jpgAnti-USP22 Rabbit Monoclonal AntibodyIHC analysis of USP22 using anti-USP22 antibody (M03887-1). USP22 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-USP22 Antibody (M03887-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03887-1-usp22-primary-antibodies-ihc-testing-3.jpgAnti-USP22 Rabbit Monoclonal AntibodyIHC analysis of USP22 using anti-USP22 antibody (M03887-1). USP22 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-USP22 Antibody (M03887-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camki-rabbit-monoclonal-antibody-m02576-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02576-wb.jpgAnti-CaMKI Rabbit Monoclonal AntibodyWestern blot analysis of CaMKI expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lrp6-rabbit-monoclonal-antibody-m00970-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00970-lrp6-primary-antibodies-wb-testing-1.jpgAnti-LRP6 Rabbit Monoclonal AntibodyWestern blot analysis of LRP6 using anti-LRP6 antibody (M00970). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRP6 antigen affinity purified monoclonal antibody (M00970) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LRP6 at approximately 230 kDa. The expected band size for LRP6 is at 180 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glua4-rabbit-monoclonal-antibody-m07044-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07044-wb.jpgAnti-GluA4 Rabbit Monoclonal AntibodyWestern blot analysis of Ionotropic Glutamate receptor 4 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab27a-rabbit-monoclonal-antibody-m01608-2-boster.html2026-03-17T05:11:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01608-2-wb.jpgAnti-RAB27A Rabbit Monoclonal AntibodyWestern blot analysis of RAB27A expression in MCF-7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-synapsin-ii-rabbit-monoclonal-antibody-m08488-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08488-wb.jpgAnti-Synapsin II Rabbit Monoclonal AntibodyWestern blot analysis of Synapsin II expression in Human fetal brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hex-rabbit-monoclonal-antibody-m02723-2-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02723-2-hhex-primary-antibodies-wb-testing-1.jpgAnti-Hex Rabbit Monoclonal AntibodyWestern blot analysis of HHEX using anti-HHEX antibody (M02723-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HHEX antigen affinity purified monoclonal antibody (M02723-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HHEX at approximately 30 kDa. The expected band size for HHEX is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nek2-rabbit-monoclonal-antibody-m01606-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01606-wb7.jpgAnti-NEK2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01606-wb.jpgAnti-NEK2 Rabbit Monoclonal AntibodyWestern blot analysis of NEK2 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hipk2-rabbit-monoclonal-antibody-m01371-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01371-hipk2-primary-antibodies-wb-testing-1.jpgAnti-HIPK2 Rabbit Monoclonal Antibody Western blot analysis of HIPK2 using anti-HIPK2 antibody (M01371). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIPK2 antigen affinity purified monoclonal antibody (Catalog # M01371) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIPK2 at approximately 100 kDa. The expected band size for HIPK2 is at 130 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irf-9-rabbit-monoclonal-antibody-m04485-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04485-wb.jpgAnti-IRF-9 Rabbit Monoclonal AntibodyWestern blot analysis of Interferon regulatory factor 9 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ebf1-rabbit-monoclonal-antibody-m01341-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01341-wb.jpgAnti-EBF1 Rabbit Monoclonal AntibodyWestern blot analysis of EBF1 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dlx5-rabbit-monoclonal-antibody-m02523-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02523-dlx5-primary-antibodies-wb-testing-1.jpgAnti-Dlx5 Rabbit Monoclonal AntibodyWestern blot analysis of DLX5 using anti-DLX5 antibody (M02523). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLX5 antigen affinity purified monoclonal antibody (M02523) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLX5 at approximately 35 kDa. The expected band size for DLX5 is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gdf3-rabbit-monoclonal-antibody-m06869-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06869-wb.jpgAnti-GDF3 Rabbit Monoclonal AntibodyWestern blot analysis of GDF3 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxl2-rabbit-monoclonal-antibody-m01185-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01185-wb.jpgAnti-FOXL2 Rabbit Monoclonal AntibodyWestern blot analysis of FOXL2 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ehd1-rabbit-monoclonal-antibody-m02168-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02168-wb.jpgAnti-EHD1 Rabbit Monoclonal AntibodyWestern blot analysis of EHD1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cntn2-tag1-rabbit-monoclonal-antibody-m04936-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04936-wb.jpgAnti-CNTN2 / TAG1 Rabbit Monoclonal AntibodyWestern blot analysis of TAG1 expression in U87-MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-npc1l1-rabbit-monoclonal-antibody-m01954-boster.html2026-03-17T05:11:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01954-wb.jpgAnti-NPC1L1 Rabbit Monoclonal AntibodyWestern blot analysis of Niemann Pick C1 Like 1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jarid2-rabbit-monoclonal-antibody-m01969-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01969-wb.jpgAnti-Jarid2 Rabbit Monoclonal AntibodyWestern blot analysis of Jarid2 expression in NCCIT cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tab1-rabbit-monoclonal-antibody-m02847-2-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02847-2-tab1-primary-antibodies-wb-testing-1.jpgAnti-TAB1 Rabbit Monoclonal AntibodyWestern blot analysis of TAB1 using anti-TAB1 antibody (M02847-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB1 antigen affinity purified monoclonal antibody (M02847-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAB1 at approximately 55 kDa. The expected band size for TAB1 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-monoamine-oxidase-a-rabbit-monoclonal-antibody-m03326-1-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03326-1-wb7.jpgAnti-Monoamine Oxidase A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03326-1-wb10.jpgAnti-Monoamine Oxidase A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03326-1-wb8.jpgAnti-Monoamine Oxidase A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03326-1-wb11.jpgAnti-Monoamine Oxidase A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03326-1-wb9.jpgAnti-Monoamine Oxidase A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03326-1-wb12.jpgAnti-Monoamine Oxidase A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03326-1-wb.jpgAnti-Monoamine Oxidase A Rabbit Monoclonal AntibodyWestern blot analysis of Monoamine Oxidase A expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bmp6-rabbit-monoclonal-antibody-m06924-1-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06924-1-12903_2025_5661_fig6_html.pngAnti-BMP6 Rabbit Monoclonal AntibodyTarget three key gene loci of two key tsRNAs in NSCPO tissues. ( a ) 5’tiRNA-35-GlyTCC-3 targets BMP6. ( b ) 5’tiRNA-35-GlyTCC-3 targets CUL1. ( c ) 5’tiRNA-33-CysGCA-11 targets SPR Index in PubMed under a CC BY license. PMID: <a href='https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-025-05661-8'>40012056</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06924-1-12903_2025_5661_fig9_html.pngAnti-BMP6 Rabbit Monoclonal AntibodyRelative expression of three key mRNAs SPR ( a ), BMP6 ( b ) and CUL1 ( c ) in clinical samples by RT-qPCR. (**** p < 0.0001; *** p < 0.001; ** p < 0.01) Index in PubMed under a CC BY license. PMID: <a href='https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-025-05661-8'>40012056</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06924-1-12903_2025_5661_fig10_html.pngAnti-BMP6 Rabbit Monoclonal AntibodyImmunohistochemistry staining of BMP6, CUL1, SPR in the Health, Ctrl and NSCPO tissue Index in PubMed under a CC BY license. PMID: <a href='https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-025-05661-8'>40012056</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06924-1-13020_2025_1062_fig5_html.pngAnti-BMP6 Rabbit Monoclonal AntibodyBMP6 expression in different cellular subpopulations. A Differential expression of BMP6 in various cell types of the heart. B Differential gene expression in fibroblasts between DCM and NH. C Differential gene expression in endothelial cells between DCM and NH. D Elbow diagram obtained using the harmony method and we selected 0–15 as the number of dimensions. E Specific markers and expression levels in fibroblasts and myofibroblasts. F – I subdividing and annotating fibroblast subpopulations by TSNE and Umap methods. J BMP6 expression between conventional fibroblasts and myofibroblasts. K differential expression of BMP6 in fibroblasts between DCM and NH. L Specific markers and expression levels of macrophage subpopulations. M – P Subdividing and annotating macrophage subpopulations by TSNE and Umap methods. Q Differences in the percentage of macrophage subpopulations abundance between DCM and NH Index in PubMed under a CC BY license. PMID: <a href='https://cmjournal.biomedcentral.com/articles/10.1186/s13020-025-01062-9'>39825396</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06924-1-13020_2025_1062_fig6_html.pngAnti-BMP6 Rabbit Monoclonal AntibodyMendelian randomization analysis between BMP6 and DCM. A Scatter trend plot of effective SNPs. B The black line represents the nature of each SNP, and the red lines represent the combined nature of the SNPs analyzed by the IVW and MR Egger methods, respectively. C SNPs distribution map. D Leave-one-out analysis. The red line shows the results of the combined analysis Index in PubMed under a CC BY license. PMID: <a href='https://cmjournal.biomedcentral.com/articles/10.1186/s13020-025-01062-9'>39825396</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06924-1-13020_2025_1062_fig8_html.pngAnti-BMP6 Rabbit Monoclonal AntibodyImmunofluorescence staining and cardiac tissue protein assay and BMP6 knockdown. A Immunofluorescence staining of markers specific to macrophages and type 2 macrophages. B and C Western blot was used to detect the changes in protein levels in the cardiac tissues between DCM and NC (n = 3). D and E The knockdown of BMP6 and the expression levels of SMAD6 and COL1A1 (n = 3). The siRNA group represents the knockdown group, si-NC represents the empty vector group, and blank represents the control group. The significance of * P in the above graph: * P < 0.05; ** P < 0.01. F Potential interventional herbs based on BMP6 inverse prediction Index in PubMed under a CC BY license. PMID: <a href='https://cmjournal.biomedcentral.com/articles/10.1186/s13020-025-01062-9'>39825396</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06924-1-bmp6-primary-antibodies-wb-testing-1_1.jpgAnti-BMP6 Rabbit Monoclonal Antibody Western blot analysis of BMP6 using anti-BMP6 antibody (M06924-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP6 antigen affinity purified monoclonal antibody (Catalog # M06924-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP6 at approximately 42 kDa. The expected band size for BMP6 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pcb-rabbit-monoclonal-antibody-m03853-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03853-wb.jpgAnti-PCB Rabbit Monoclonal AntibodyWestern blot analysis of PCB expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ifngr1-rabbit-monoclonal-antibody-m01716-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01716-wb.jpgAnti-IFNGR1 Rabbit Monoclonal AntibodyWestern blot analysis of IFNGR1 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rap1gap-rabbit-monoclonal-antibody-m03561-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03561-rap1gap-primary-antibodies-wb-testing-1.jpgAnti-RAP1GAP Rabbit Monoclonal AntibodyWestern blot analysis of RAP1GAP using anti-RAP1GAP antibody (M03561). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GAP antigen affinity purified monoclonal antibody (M03561) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAP1GAP at approximately 95 kDa. The expected band size for RAP1GAP is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-estrogen-receptor-alpha-rabbit-monoclonal-antibody-m00057-4-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00057-4-eresr1-primary-antibodies-wb-testing-1.jpgAnti-Estrogen Receptor alpha Rabbit Monoclonal AntibodyWestern blot analysis of ERESR1 using anti-ERESR1 antibody (M00057-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERESR1 antigen affinity purified monoclonal antibody (M00057-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERESR1 at approximately 66 kDa. The expected band size for ERESR1 is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsf1-rabbit-monoclonal-antibody-m00250-1-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00250-1-hsf1-primary-antibodies-wb-testing-1.jpgAnti-HSF1 Rabbit Monoclonal Antibody Western blot analysis of HSF1 using anti-HSF1 antibody (M00250-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 antigen affinity purified monoclonal antibody (Catalog # M00250-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF1 at approximately 80 kDa. The expected band size for HSF1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00250-1-hsf1-primary-antibodies-ihc-testing-2.jpgAnti-HSF1 Rabbit Monoclonal Antibody IHC analysis of HSF1 using anti-HSF1 antibody (M00250-1). HSF1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HSF1 Antibody (M00250-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00250-1-hsf1-primary-antibodies-ihc-testing-3.jpgAnti-HSF1 Rabbit Monoclonal Antibody IHC analysis of HSF1 using anti-HSF1 antibody (M00250-1). HSF1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HSF1 Antibody (M00250-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sqstm1-p62-rabbit-monoclonal-antibody-m00300-3-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-3_41598_2025_2125_fig4_html.pngAnti-SQSTM1 / p62 Rabbit Monoclonal AntibodyInetetamab regulated the expression levels of apoptosis- and autophagy-related proteins of the H9c2 cells in a dose-dependant manner. ( A ) Inetetamab modulated the expression levels of P62, Beclin 1, Caspase-3, Bcl-2 and Bax proteins in the H9c2 cells. ( B ) The quantification of the aforementioned proteins was conducted in the H9c2 cells from A ( n = 3). ** P < 0.01 vs. Control, ▲▲ P < 0.01 vs. 2.5 mg/ml, ## P < 0.01 vs. 4.5 mg/ml. Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-025-02125-5'>40593780</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-3_41598_2025_2125_fig6_html.pngAnti-SQSTM1 / p62 Rabbit Monoclonal AntibodyInetetamab induced myocardial injury in mice through the enhancement of apoptosis and autophagy in a dose-dependent manner. ( A ) Hochest 33,342 staining showed that Inetetamab enhanced myocardial cell apoptosis. ( B , D ) Immunohistochemistry analysis demonstrated that Inetetamab upregulated the expression levels of Bax and Caspase-3 proteins in the myocardial tissues. ( C , E ) The quantification of Bax and Caspase-3 levels was determined from B and D ( n = 5). ( F ) Inetetamab enhanced MDA levels in the myocardial tissues ( n = 5). ( G ) Inetetamab reduced SOD levels in the myocardial tissues ( n = 5). ( H ) Inetetamab reduced the GSH/GSSH ratio in the myocardial tissues ( n = 5). ( I ) Inetetamab modulated the expression levels of P62, Beclin 1, Caspase-3, Bcl-2 and Bax proteins in the myocardial tissues. ( J ) The quantification of the aforementioned proteins was conducted from I ( n = 3). ** P < 0.01 vs. Control, ▲▲ P < 0.01 vs. 4 mg/ml. Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-025-02125-5'>40593780</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-3-sqstm1-primary-antibodies-wb-testing-1.jpgAnti-SQSTM1 / p62 Rabbit Monoclonal Antibody Western blot analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (M00300-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P62/SQSTM1 antigen affinity purified monoclonal antibody (M00300-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P62/SQSTM1 at approximately 62 kDa. The expected band size for P62/SQSTM1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-3-sqstm1-primary-antibodies-ihc-testing-2.jpgAnti-SQSTM1 / p62 Rabbit Monoclonal Antibody IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (M00300-3) . P62/SQSTM1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P62/SQSTM1 Antibody (M00300-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-3-sqstm1-primary-antibodies-ihc-testing-3.jpgAnti-SQSTM1 / p62 Rabbit Monoclonal Antibody IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (M00300-3) . P62/SQSTM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P62/SQSTM1 Antibody (M00300-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-3-sqstm1-primary-antibodies-ihc-testing-4.jpgAnti-SQSTM1 / p62 Rabbit Monoclonal Antibody IHC analysis of P62/SQSTM1 using anti-P62/SQSTM1 antibody (M00300-3) . P62/SQSTM1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P62/SQSTM1 Antibody (M00300-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmp1-rabbit-monoclonal-antibody-m00733-2-boster.html2026-03-17T05:11:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00733-2-wb.jpgAnti-MMP1 Rabbit Monoclonal AntibodyWestern blot analysis of MMP1 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcm7-rabbit-monoclonal-antibody-m01649-2-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-2-wb.jpgAnti-MCM7 Rabbit Monoclonal AntibodyWestern blot analysis of MCM7 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-2-kd1.jpgAnti-MCM7 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-actn3-rabbit-monoclonal-antibody-m02693-1-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02693-1-wb.jpgAnti-ACTN3 Rabbit Monoclonal AntibodyWestern blot analysis of ACTN3 expression in mouse skeletal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trib3-rabbit-monoclonal-antibody-m01414-2-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01414-2-wb.jpgAnti-TRIB3 Rabbit Monoclonal AntibodyWestern blot analysis of TRIB3 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-raidd-rabbit-monoclonal-antibody-m06509-2-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06509-2-wb.jpgAnti-RAIDD Rabbit Monoclonal AntibodyWestern blot analysis of RAIDD expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sam68-rabbit-monoclonal-antibody-m01717-1-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01717-1-wb7.jpgAnti-SAM68 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01717-1-wb.jpgAnti-SAM68 Rabbit Monoclonal AntibodyWestern blot analysis of SAM68 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gnb2-rabbit-monoclonal-antibody-m03535-1-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03535-1-gnb2-primary-antibodies-wb-testing-1.jpgAnti-GNB2 Rabbit Monoclonal AntibodyWestern blot analysis of GNB2 using anti-GNB2 antibody (M03535-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse small intestine tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNB2 antigen affinity purified monoclonal antibody (M03535-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNB2 at approximately 35 kDa. The expected band size for GNB2 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufaf1-rabbit-monoclonal-antibody-m10145-1-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10145-1-wb.jpgAnti-NDUFAF1 Rabbit Monoclonal AntibodyWestern blot analysis of NDUFAF1 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tc10-rabbit-monoclonal-antibody-m06900-1-boster.html2026-03-17T05:11:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06900-1-wb.jpgAnti-TC10 Rabbit Monoclonal AntibodyWestern blot analysis of TC10 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tppp-rabbit-monoclonal-antibody-m04389-1-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04389-1-wb.jpgAnti-TPPP Rabbit Monoclonal AntibodyWestern blot analysis of TPPP expression in SHSY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ap2s1-rabbit-monoclonal-antibody-m07176-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07176-wb.jpgAnti-AP2S1 Rabbit Monoclonal AntibodyWestern blot analysis of AP2S1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gstk1-rabbit-monoclonal-antibody-m06112-1-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06112-1-gstk1-primary-antibodies-wb-testing-1_1.jpgAnti-GSTK1 Rabbit Monoclonal AntibodyWestern blot analysis of GSTK1 using anti-GSTK1 antibody (M06112-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTK1 antigen affinity purified monoclonal antibody (M06112-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSTK1 at approximately 26 kDa. The expected band size for GSTK1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06112-1-gstk1-primary-antibodies-ihc-testing-1_1.jpgAnti-GSTK1 Rabbit Monoclonal AntibodyIHC analysis of GSTK1 using anti-GSTK1 antibody (M06112-1). GSTK1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GSTK1 Antibody (M06112-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06112-1-gstk1-primary-antibodies-ihc-testing-2_1.jpgAnti-GSTK1 Rabbit Monoclonal AntibodyIHC analysis of GSTK1 using anti-GSTK1 antibody (M06112-1). GSTK1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GSTK1 Antibody (M06112-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06112-1-gstk1-primary-antibodies-ihc-testing-3_1.jpgAnti-GSTK1 Rabbit Monoclonal AntibodyIHC analysis of GSTK1 using anti-GSTK1 antibody (M06112-1). GSTK1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GSTK1 Antibody (M06112-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06112-1-gstk1-primary-antibodies-ihc-testing-4_1.jpgAnti-GSTK1 Rabbit Monoclonal AntibodyIHC analysis of GSTK1 using anti-GSTK1 antibody (M06112-1). GSTK1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GSTK1 Antibody (M06112-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gtpbp4-rabbit-monoclonal-antibody-m10450-1-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10450-1-wb.jpgAnti-GTPBP4 Rabbit Monoclonal AntibodyWestern blot analysis of GTPBP4 expression in Human fetal kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cops3-rabbit-monoclonal-antibody-m07987-1-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07987-1-wb.jpgAnti-COPS3 Rabbit Monoclonal AntibodyWestern blot analysis of COPS3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-placental-protein-14-paep-rabbit-monoclonal-antibody-m06051-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06051-wb.jpgAnti-Placental Protein 14 / PAEP Rabbit Monoclonal AntibodyWestern blot analysis of Placental Protein 14 / PAEP expression in TPA-treated K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bag2-rabbit-monoclonal-antibody-m04933-1-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04933-1-wb.jpgAnti-BAG2 Rabbit Monoclonal AntibodyWestern blot analysis of BAG2 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lysophospholipase-1-rabbit-monoclonal-antibody-m07249-1-boster.html2026-03-17T05:11:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07249-1-wb.jpgAnti-Lysophospholipase 1 Rabbit Monoclonal AntibodyWestern blot analysis of Lysophospholipase 1 expression in Human fetal liver lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thyroglobulin-rabbit-monoclonal-antibody-m00359-6-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00359-6-wb.jpgAnti-Thyroglobulin Rabbit Monoclonal AntibodyWestern blot analysis of Thyroglobulin expression in Human thyroid lysate lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apolipoprotein-f-rabbit-monoclonal-antibody-m07726-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07726-wb.jpgAnti-Apolipoprotein F Rabbit Monoclonal AntibodyWestern blot analysis of Apolipoprotein F expression in human plasma cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calpain-small-subunit-1-rabbit-monoclonal-antibody-m04111-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04111-wb.jpgAnti-Calpain small subunit 1 Rabbit Monoclonal AntibodyWestern blot analysis of Calpain small subunit 1 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-otx2-rabbit-monoclonal-antibody-m02006-1-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02006-1-wb.jpgAnti-Otx2 Rabbit Monoclonal AntibodyWestern blot analysis of Otx2 expression in Y79 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bob1-rabbit-monoclonal-antibody-m04431-3-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04431-3-wb.jpgAnti-BOB1 Rabbit Monoclonal AntibodyWestern blot analysis of BOB1 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-saa4-rabbit-monoclonal-antibody-m07115-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07115-wb.jpgAnti-SAA4 Rabbit Monoclonal AntibodyWestern blot analysis of SAA4 expression in Human plasma cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-entpd5-rabbit-monoclonal-antibody-m06908-1-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06908-1-wb.jpgAnti-ENTPD5 Rabbit Monoclonal AntibodyWestern blot analysis of ENTPD5 expression in LnCaP cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pon1-rabbit-monoclonal-antibody-m00516-6-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00516-6-wb.jpgAnti-PON1 Rabbit Monoclonal AntibodyWestern blot analysis of PON1 expression in human plasma cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-isg15-rabbit-monoclonal-antibody-m00554-2-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-2-wb.jpgAnti-ISG15 Rabbit Monoclonal AntibodyWestern blot analysis of ISG15 expression in HeLa cell treated with 10 ng/ml IFN-a lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tryptophanyl-trna-synthetase-rabbit-monoclonal-antibody-m02444-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02444-wb.jpgAnti-Tryptophanyl tRNA synthetase Rabbit Monoclonal AntibodyWestern blot analysis of Tryptophanyl tRNA synthetase expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acadm-rabbit-monoclonal-antibody-m02383-2-boster.html2026-03-17T05:11:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02383-2-wb.jpgAnti-ACADM Rabbit Monoclonal AntibodyWestern blot analysis of ACADM expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cend1-rabbit-monoclonal-antibody-m13043-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13043-cend1-primary-antibodies-wb-testing-1.jpgAnti-CEND1 Rabbit Monoclonal Antibody Western blot analysis of CEND1 using anti-CEND1 antibody (M13043). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEND1 antigen affinity purified monoclonal antibody (Catalog # M13043) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEND1 at approximately 22 kDa. The expected band size for CEND1 is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mad3-rabbit-monoclonal-antibody-m11285-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11285-wb.jpgAnti-MAD3 Rabbit Monoclonal AntibodyWestern blot analysis of MAD3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pcyt1a-rabbit-monoclonal-antibody-m04091-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04091-wb.jpgAnti-PCYT1A Rabbit Monoclonal AntibodyWestern blot analysis of PCYT1A expression in A549 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04091-wb7.jpgAnti-PCYT1A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsf2-rabbit-monoclonal-antibody-m04692-1-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04692-1-wb.jpgAnti-HSF2 Rabbit Monoclonal AntibodyWestern blot analysis of HSF2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcp1-alpha-rabbit-monoclonal-antibody-m02389-2-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02389-2-wb.jpgAnti-TCP1 alpha Rabbit Monoclonal AntibodyWestern blot analysis of TCP1 alpha expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gcdfp-15-rabbit-monoclonal-antibody-m04543-2-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04543-2-wb.jpgAnti-GCDFP 15 Rabbit Monoclonal AntibodyWestern blot analysis of GCDFP 15 expression in T47D cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cripto1-rabbit-monoclonal-antibody-m03105-1-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03105-1-wb7.jpgAnti-Cripto1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03105-1-wb.jpgAnti-Cripto1 Rabbit Monoclonal AntibodyWestern blot analysis of Cripto1 expression in NCCIT cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fbp1-rabbit-monoclonal-antibody-m01377-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01377-mbp1-primary-antibodies-wb-testing-1.jpgAnti-FBP1 Rabbit Monoclonal Antibody Western blot analysis of ACVR1 using anti-ACVR1 antibody (M01377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR1 antigen affinity purified monoclonal antibody (Catalog # M01377) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR1 at approximately 37 kDa. The expected band size for ACVR1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01377-mbp1-primary-antibodies-wb-testing-2.jpgAnti-FBP1 Rabbit Monoclonal Antibody Western blot analysis of ACVR1 using anti-ACVR1 antibody (M01377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR1 antigen affinity purified monoclonal antibody (Catalog # M01377) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR1 at approximately 37 kDa. The expected band size for ACVR1 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rsk1-p90-rabbit-monoclonal-antibody-m01058-2-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-2-wb.jpgAnti-RSK1 p90 Rabbit Monoclonal AntibodyWestern blot analysis of RSK1 p90 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clstn1-rabbit-monoclonal-antibody-m07157-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07157-wb.jpgAnti-CLSTN1 Rabbit Monoclonal AntibodyWestern blot analysis of CLSTN1 expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07157-clstn1-primary-antibodies-ihc-testing-1.jpgAnti-CLSTN1 Rabbit Monoclonal AntibodyIHC analysis of Calsyntenin-1/CLSTN1 using anti-Calsyntenin-1/CLSTN1 antibody (M07157). Calsyntenin-1/CLSTN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Calsyntenin-1/CLSTN1 Antibody (M07157) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmd14-rabbit-monoclonal-antibody-m06584-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06584-psdm14-primary-antibodies-wb-testing-1.jpgAnti-PSMD14 Rabbit Monoclonal AntibodyWestern blot analysis of PSDM14 using anti-PSDM14 antibody (M06584). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSDM14 antigen affinity purified monoclonal antibody (M06584) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSDM14 at approximately 35 kDa. The expected band size for PSDM14 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-entpd5-rabbit-monoclonal-antibody-m06908-2-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06908-2-wb.jpgAnti-ENTPD5 Rabbit Monoclonal AntibodyWestern blot analysis of ENTPD5 expression in fetal liver lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plastin-l-rabbit-monoclonal-antibody-m03361-2-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03361-2-wb.jpgAnti-Plastin L Rabbit Monoclonal AntibodyWestern blot analysis of Plastin L expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-synapsin-ii-rabbit-monoclonal-antibody-m08488-1-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08488-1-wb.jpgAnti-Synapsin II Rabbit Monoclonal AntibodyWestern blot analysis of Synapsin II expression in Neuro-2a cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-april-rabbit-monoclonal-antibody-m02417-2-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02417-2-wb.jpgAnti-APRIL Rabbit Monoclonal AntibodyWestern blot analysis of APRIL expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rsk4-rabbit-monoclonal-antibody-m07352-boster.html2026-03-17T05:11:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07352-wb.jpgAnti-RSK4 Rabbit Monoclonal AntibodyWestern blot analysis of RSK4 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-icad-rabbit-monoclonal-antibody-m03671-1-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03671-1-wb.jpgAnti-ICAD Rabbit Monoclonal AntibodyWestern blot analysis of ICAD expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pka-r2-rabbit-monoclonal-antibody-m03911-4-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-ppkar2a-primary-antibodies-wb-testing-1.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyWestern blot analysis of PPKAR2A using anti-PPKAR2A antibody (M03911-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPKAR2A antigen affinity purified monoclonal antibody (M03911-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPKAR2A at approximately 52 kDa. The expected band size for PPKAR2A is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-1.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-2.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-3.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-4.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-5.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-6.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-7.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03911-4-prkar2a-primary-antibodies-ihc-testing-8.jpgAnti-PKA R2 Rabbit Monoclonal AntibodyIHC analysis of PRKAR2A using anti-PRKAR2A antibody (M03911-4). PRKAR2A was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PRKAR2A Antibody (M03911-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gpa33-rabbit-monoclonal-antibody-m07688-1-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07688-1-wb.jpgAnti-GPA33 Rabbit Monoclonal AntibodyWestern blot analysis of GPA33 expression in HT-29 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ewsr1-rabbit-monoclonal-antibody-m00589-2-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00589-2-wb.jpgAnti-EWSR1 Rabbit Monoclonal AntibodyWestern blot analysis of EWSR1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lactoferrin-rabbit-monoclonal-antibody-m00633-2-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00633-2-wb.jpgAnti-Lactoferrin Rabbit Monoclonal AntibodyWestern blot analysis of Lactoferrin expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mbd2-rabbit-monoclonal-antibody-m01746-2-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01746-2-wb.jpgAnti-MBD2 Rabbit Monoclonal AntibodyWestern blot analysis of MBD2 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd16-rabbit-monoclonal-antibody-m01408-1-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01408-1-cd16a-primary-antibodies-wb-testing-1.jpgAnti-CD16 Rabbit Monoclonal Antibody Western blot analysis of CD16 using anti-CD16 antibody (M01408-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD16 antigen affinity purified monoclonal antibody (Catalog # M01408-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD16 at approximately 42 kDa. The expected band size for CD16 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01408-1-wb.jpgAnti-CD16 Rabbit Monoclonal AntibodyWestern blot analysis of CD16 expression in THP-1 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01408-1-wb8.jpgAnti-CD16 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5k dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nrg3-rabbit-monoclonal-antibody-m06681-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06681-wb.jpgAnti-NRG3 Rabbit Monoclonal AntibodyWestern blot analysis of NRG3 expression in (1) SH-SY5Y cell lysate; (2) C6 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oct-2-rabbit-monoclonal-antibody-m04407-2-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04407-2-wb.jpgAnti-Oct-2 Rabbit Monoclonal AntibodyWestern blot analysis of Oct-2 expression in Daudi cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-abat-rabbit-monoclonal-antibody-m07133-2-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07133-2-wb.jpgAnti-ABAT Rabbit Monoclonal AntibodyWestern blot analysis of ABAT expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp1b3-rabbit-monoclonal-antibody-m08492-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08492-wb.jpgAnti-ATP1B3 Rabbit Monoclonal AntibodyWestern blot analysis of ATP1B3 expression in mouse brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xlf-rabbit-monoclonal-antibody-m03552-2-boster.html2026-03-17T05:11:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03552-2-wb.jpgAnti-XLF Rabbit Monoclonal AntibodyWestern blot analysis of XLF expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trkb-rabbit-monoclonal-antibody-m01388-3-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01388-3-gr7.jpgAnti-TrkB Rabbit Monoclonal AntibodyResults of western blotting (n = 3). (A) Relative expressions of GR, SERT, BDNF, Trk B, and p-Trk B in hippocampal tissues of GS + MS and PS + MS groups; Relative gray values of (B) GR, (C) SERT, and (D) BDNF and (E) the ratio of p-Trk B to Trk B in hippocampal tissues of female mice. Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)11394-1'>39166014</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01388-3-dddt_a_12469766_f0007_c.jpgAnti-TrkB Rabbit Monoclonal AntibodyVerification results of the WB and ELISA test. Notes: (A) Test procedure; (B) WB strip map; (C) WB gray value; (D) ELISA test results. The data are shown as the means ± SDs (n = 5). *P < 0.05, **P < 0.01 and ***P < 0.001 denote significant differences from the control group. #P < 0.05, ##P < 0.01 and ###P < 0.001 denote significant differences from the Model group. Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/DDDT.S537918'>40859969</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01388-3-trkb-primary-antibodies-wb-testing-1.jpgAnti-TrkB Rabbit Monoclonal Antibody Western blot analysis of TrkB using anti-TrkB antibody (M01388-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkB antigen affinity purified monoclonal antibody (Catalog # M01388-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkB at approximately 92,140 kDa. The expected band size for TrkB is at 92 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pmca1-rabbit-monoclonal-antibody-m02669-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02669-pmca1-primary-antibodies-wb-testing-1.jpgAnti-PMCA1 Rabbit Monoclonal Antibody Western blot analysis of PMCA1 using anti-PMCA1 antibody (M02669). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMCA1 antigen affinity purified monoclonal antibody (Catalog # M02669) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMCA1 at approximately 250 kDa. The expected band size for PMCA1 is at 135 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tin2-rabbit-monoclonal-antibody-m03070-1-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03070-1-wb.jpgAnti-Tin2 Rabbit Monoclonal AntibodyWestern blot analysis of Tin2 expression in (1) HUVEC cell lysate; (2) NIH/3T3 cell lysate; (3) PC12 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gstm1-rabbit-monoclonal-antibody-m00569-1-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00569-1-wb.jpgAnti-GSTM1 Rabbit Monoclonal AntibodyWestern blot analysis of GSTM1 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-odc1-rabbit-monoclonal-antibody-m03138-2-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03138-2-wb7.jpgAnti-ODC1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03138-2-wb8.jpgAnti-ODC1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03138-2-wb.jpgAnti-ODC1 Rabbit Monoclonal AntibodyWestern blot analysis of ODC1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sorla-rabbit-monoclonal-antibody-m01887-2-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01887-2-wb.jpgAnti-SorLA Rabbit Monoclonal AntibodyWestern blot analysis of SorLA expression in SH-SY5Y cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01887-2-sorla-primary-antibodies-ihc-testing-1.jpgAnti-SorLA Rabbit Monoclonal AntibodyIHC analysis of SORLA using anti-SORLA antibody (M01887-2). SORLA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SORLA Antibody (M01887-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01887-2-sorla-primary-antibodies-ihc-testing-2.jpgAnti-SorLA Rabbit Monoclonal AntibodyIHC analysis of SORLA using anti-SORLA antibody (M01887-2). SORLA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SORLA Antibody (M01887-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01887-2-sorla-primary-antibodies-ihc-testing-3.jpgAnti-SorLA Rabbit Monoclonal AntibodyIHC analysis of SORLA using anti-SORLA antibody (M01887-2). SORLA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SORLA Antibody (M01887-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01887-2-sorla-primary-antibodies-ihc-testing-4.jpgAnti-SorLA Rabbit Monoclonal AntibodyIHC analysis of SORLA using anti-SORLA antibody (M01887-2). SORLA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SORLA Antibody (M01887-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnase-l-rabbit-monoclonal-antibody-m02521-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02521-wb.jpgAnti-RNase L Rabbit Monoclonal AntibodyWestern blot analysis of RNase L expression in THP1 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tnfsf9-rabbit-monoclonal-antibody-m06032-1-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06032-1-wb.jpgAnti-TNFSF9 Rabbit Monoclonal AntibodyWestern blot analysis of TNFSF9 expression in HEK293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcp1-beta-rabbit-monoclonal-antibody-m05524-2-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05524-2-cct2-primary-antibodies-wb-testing-1_1.jpgAnti-TCP1 beta Rabbit Monoclonal Antibody Western blot analysis of TCP1 Beta using anti-TCP1 Beta antibody (M05524-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCP1 Beta antigen affinity purified monoclonal antibody (Catalog # M05524-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCP1 Beta at approximately 57 kDa. The expected band size for TCP1 Beta is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-epac2-rabbit-monoclonal-antibody-m04794-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04794-wb.jpgAnti-Epac2 Rabbit Monoclonal AntibodyWestern blot analysis of Epac2 expression in Human fetal brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04794-epac2-primary-antibodies-ihc-testing-1_1.jpgAnti-Epac2 Rabbit Monoclonal AntibodyIHC analysis of EPAC2 using anti-EPAC2 antibody (M04794). EPAC2 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EPAC2 Antibody (M04794) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04794-epac2-primary-antibodies-ihc-testing-2_1.jpgAnti-Epac2 Rabbit Monoclonal AntibodyIHC analysis of EPAC2 using anti-EPAC2 antibody (M04794). EPAC2 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EPAC2 Antibody (M04794) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04794-epac2-primary-antibodies-ihc-testing-3_1.jpgAnti-Epac2 Rabbit Monoclonal AntibodyIHC analysis of EPAC2 using anti-EPAC2 antibody (M04794). EPAC2 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EPAC2 Antibody (M04794) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spop-rabbit-monoclonal-antibody-m02032-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02032-spop-primary-antibodies-wb-testing-1.jpgAnti-SPOP Rabbit Monoclonal AntibodyWestern blot analysis of SPOP using anti-SPOP antibody (M02032). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOP antigen affinity purified monoclonal antibody (M02032) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPOP at approximately 42 kDa. The expected band size for SPOP is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tead1-2-3-4-rabbit-monoclonal-antibody-m03263-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03263-tead-primary-antibodies-wb-testing-1.jpgAnti-TEAD1/2/3/4 Rabbit Monoclonal AntibodyWestern blot analysis of TEAD1/2/3/4 using anti-TEAD1/2/3/4 antibody (M03263). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD1/2/3/4 antigen affinity purified monoclonal antibody (M03263) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TEAD1/2/3/4 at approximately 54 kDa. The expected band size for TEAD1/2/3/4 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apolipoprotein-ci-rabbit-monoclonal-antibody-m02526-boster.html2026-03-17T05:11:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02526-apoc1-primary-antibodies-wb-testing-1.jpgAnti-Apolipoprotein CI Rabbit Monoclonal Antibody Western blot analysis of Apolipoprotein CI using anti-Apolipoprotein CI antibody (M02526). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein CI antigen affinity purified monoclonal antibody (Catalog # M02526) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein CI at approximately 9 kDa. The expected band size for Apolipoprotein CI is at 9 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stk33-rabbit-monoclonal-antibody-m07375-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07375-stk33-primary-antibodies-wb-testing-1.jpgAnti-STK33 Rabbit Monoclonal Antibody Western blot analysis of STK33 using anti-STK33 antibody (M07375). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK33 antigen affinity purified monoclonal antibody (M07375) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STK33 at approximately 58 kDa. The expected band size for STK33 is at 58, 50 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikzf3-rabbit-monoclonal-antibody-m01611-2-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01611-2-ikzf3-primary-antibodies-wb-testing-1.jpgAnti-IKZF3 Rabbit Monoclonal AntibodyFigure 1. Western blot analysis of IKZF3 using anti-IKZF3 antibody (M01611-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF3 antigen affinity purified monoclonal antibody (M01611-2) at a dilution of 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IKZF3 at approximately 70 kDa. The expected band size for IKZF3 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01611-2-ikzf3-primary-antibodies-ihc-testing-2.jpgAnti-IKZF3 Rabbit Monoclonal AntibodyFigure 2. IHC analysis of IKZF3 using anti-IKZF3 antibody (M01611-2). IKZF3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-IKZF3 Antibody (M01611-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mth1-rabbit-monoclonal-antibody-m04258-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04258-mth1-primary-antibodies-wb-testing-1.jpgAnti-MTH1 Rabbit Monoclonal AntibodyWestern blot analysis of MTH1 using anti-MTH1 antibody (M04258). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTH1 antigen affinity purified monoclonal antibody (M04258) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MTH1 at approximately 18 kDa. The expected band size for MTH1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04258-nudt1-primary-antibodies-wb-testing-2.jpgAnti-MTH1 Rabbit Monoclonal AntibodyWestern blot analysis of NUDT1 using anti-NUDT1 antibody (M04258). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT1 antigen affinity purified monoclonal antibody (M04258) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUDT1 at approximately 18 kDa. The expected band size for NUDT1 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aly-rabbit-monoclonal-antibody-m03580-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-wb-testing-1.jpgAnti-Aly Rabbit Monoclonal AntibodyWestern blot analysis of ALY using anti-ALY antibody (M03580). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALY antigen affinity purified monoclonal antibody (M03580) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALY at approximately 32 kDa. The expected band size for ALY is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-1.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-2.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-3.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-4.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-5.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-6.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-7.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-8.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-9.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-10.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-11.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-12.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-13.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03580-aly-primary-antibodies-ihc-testing-14.jpgAnti-Aly Rabbit Monoclonal AntibodyIHC analysis of ALY using anti-ALY antibody (M03580). ALY was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ALY Antibody (M03580) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-inhibin-alpha-rabbit-monoclonal-antibody-m02413-2-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02413-2-wb.jpgAnti-Inhibin alpha Rabbit Monoclonal AntibodyWestern blot analysis of Inhibin alpha expression in Human testis lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spink1-rabbit-monoclonal-antibody-m00982-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00982-wb.jpgAnti-SPINK1 Rabbit Monoclonal AntibodyWestern blot analysis of SPINK1 expression in Human pancreas lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fanca-rabbit-monoclonal-antibody-m03662-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03662-wb.jpgAnti-FANCA Rabbit Monoclonal AntibodyWestern blot analysis of FANCA expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tnni1-rabbit-monoclonal-antibody-m07080-1-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07080-1-wb.jpgAnti-TNNI1 Rabbit Monoclonal AntibodyWestern blot analysis of TNNI1 expression in Human fetal heart lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tyro3-rabbit-monoclonal-antibody-m00913-5-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00913-5-tyro3-primary-antibodies-wb-testing-1.jpgAnti-TYRO3 Rabbit Monoclonal Antibody Western blot analysis of TYRO3 using anti-TYRO3 antibody (M00913-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRO3 antigen affinity purified monoclonal antibody (Catalog # M00913-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYRO3 at approximately 120 kDa. The expected band size for TYRO3 is at 97 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smc4-rabbit-monoclonal-antibody-m04887-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04887-wb.jpgAnti-SMC4 Rabbit Monoclonal AntibodyWestern blot analysis of SMC4 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-activin-receptor-type-iib-acvr2b-rabbit-monoclonal-antibody-m04653-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04653-wb.jpgAnti-Activin Receptor Type IIB/ACVR2B Rabbit Monoclonal AntibodyWestern blot analysis of Activin Receptor Type IIB/ACVR2B expression in (1) K562 cell lysate; (2) Mouse heart lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klf10-rabbit-monoclonal-antibody-m03419-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03419-wb.jpgAnti-KLF10 Rabbit Monoclonal AntibodyWestern blot analysis of KLF10 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ring1-rabbit-monoclonal-antibody-m01824-1-boster.html2026-03-17T05:11:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01824-1-wb.jpgAnti-RING1 Rabbit Monoclonal AntibodyWestern blot analysis of RING1 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rgs18-rabbit-monoclonal-antibody-m10844-1-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10844-1-wb.jpgAnti-RGS18 Rabbit Monoclonal AntibodyWestern blot analysis of RGS18 expression in Human PBMC lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tomm22-tom22-rabbit-monoclonal-antibody-m08668-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08668-tomm22-primary-antibodies-wb-testing-1.jpgAnti-TOMM22/TOM22 Rabbit Monoclonal Antibody Western blot analysis of CLIC4 using anti-CLIC4 antibody (M08668). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC4 antigen affinity purified monoclonal antibody (Catalog # M08668) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIC4 at approximately 18 kDa. The expected band size for CLIC4 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08668-icc1.jpgAnti-TOMM22/TOM22 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08668-icc2.jpgAnti-TOMM22/TOM22 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08668-icc3.jpgAnti-TOMM22/TOM22 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:500 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cpeb1-rabbit-monoclonal-antibody-m03578-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03578-cpeb1-primary-antibodies-wb-testing-1.jpgAnti-CPEB1 Rabbit Monoclonal Antibody Western blot analysis of CPEB1 using anti-CPEB1 antibody (M03578). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPEB1 antigen affinity purified monoclonal antibody (Catalog # M03578) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPEB1 at approximately 70 kDa. The expected band size for CPEB1 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oxytocin-receptor-rabbit-monoclonal-antibody-m01566-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01566-oxtr-primary-antibodies-ihc-testing-2.jpgAnti-Oxytocin Receptor Rabbit Monoclonal Antibody IHC analysis of OXTR using anti-OXTR antibody (M01566). OXTR was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-OXTR Antibody (M01566) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01566-oxtr-primary-antibodies-ihc-testing-3.jpgAnti-Oxytocin Receptor Rabbit Monoclonal Antibody IHC analysis of OXTR using anti-OXTR antibody (M01566). OXTR was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-OXTR Antibody (M01566) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01566-oxtr-primary-antibodies-wb-testing-1.jpgAnti-Oxytocin Receptor Rabbit Monoclonal Antibody Western blot analysis of OXTR using anti-OXTR antibody (M01566). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXTR antigen affinity purified monoclonal antibody (Catalog # M01566) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXTR at approximately 43 kDa. The expected band size for OXTR is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mrc2-endo180-rabbit-monoclonal-antibody-m04070-1-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04070-1-wb.jpgAnti-MRC2/ENDO180 Rabbit Monoclonal AntibodyWestern blot analysis of MRC2/ENDO180 expression in Saos2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd97-rabbit-monoclonal-antibody-m02982-1-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02982-1-wb.jpgAnti-CD97 Rabbit Monoclonal AntibodyWestern blot analysis of CD97 expression in human tonsil cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neurod2-rabbit-monoclonal-antibody-m07904-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07904-wb7.jpgAnti-NeuroD2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07904-wb.jpgAnti-NeuroD2 Rabbit Monoclonal AntibodyWestern blot analysis of NeuroD2 expression in mouse cerebellum cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nudc-rabbit-monoclonal-antibody-m00995-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00995-wb.jpgAnti-NUDC Rabbit Monoclonal AntibodyWestern blot analysis of NUDC expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fbp1-rabbit-monoclonal-antibody-m01377-1-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01377-1-wb.jpgAnti-FBP1 Rabbit Monoclonal AntibodyWestern blot analysis of FBP1 expression in MCF7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01377-1-wb9.jpgAnti-FBP1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01377-1-wb7.jpgAnti-FBP1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01377-1-wb8.jpgAnti-FBP1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ehmt2-g9a-rabbit-monoclonal-antibody-m01055-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01055-wb.jpgAnti-EHMT2/G9A Rabbit Monoclonal AntibodyWestern blot analysis of EHMT2/G9A expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-kap1-s824-rabbit-monoclonal-antibody-p00409-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00409-trim28-primary-antibodies-wb-testing-1.jpgAnti-Phospho-KAP1 (S824) Rabbit Monoclonal Antibody Western blot analysis of KAP1 using anti-KAP1 antibody (P00409). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAP1 antigen affinity purified monoclonal antibody (Catalog # P00409) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAP1 at approximately 100 kDa. The expected band size for KAP1 is at 89 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-skp2-rabbit-monoclonal-antibody-m00544-1-boster.html2026-03-17T05:11:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00544-1-wb.jpgAnti-SKP2 Rabbit Monoclonal AntibodyWestern blot analysis of SKP2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd239-rabbit-monoclonal-antibody-m03148-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03148-bcam-primary-antibodies-wb-testing-1.jpgAnti-CD239 Rabbit Monoclonal Antibody Western blot analysis of BCAM using anti-BCAM antibody (M03148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAM antigen affinity purified monoclonal antibody (M03148) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAM at approximately 85 kDa. The expected band size for BCAM is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gsc-rabbit-monoclonal-antibody-m03084-2-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03084-2-wb.jpgAnti-GSC Rabbit Monoclonal AntibodyWestern blot analysis of GSC expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-timeless-rabbit-monoclonal-antibody-m00831-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00831-timeless-primary-antibodies-wb-testing-1.jpgAnti-Timeless Rabbit Monoclonal AntibodyWestern blot analysis of TIMELESS using anti-TIMELESS antibody (M00831). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human SIHA whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMELESS antigen affinity purified monoclonal antibody (M00831) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIMELESS at approximately 150 kDa. The expected band size for TIMELESS is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00831-timeless-primary-antibodies-ihc-testing-2.jpgAnti-Timeless Rabbit Monoclonal AntibodyIHC analysis of TIMELESS using anti-TIMELESS antibody (M00831). TIMELESS was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TIMELESS Antibody (M00831) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-desmoplakin-rabbit-monoclonal-antibody-m00616-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00616-wb.jpgAnti-Desmoplakin Rabbit Monoclonal AntibodyWestern blot analysis of Desmoplakin expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bmp15-rabbit-monoclonal-antibody-m01842-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01842-wb.jpgAnti-BMP15 Rabbit Monoclonal AntibodyWestern blot analysis of BMP15 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psma2-rabbit-monoclonal-antibody-m08901-1-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08901-1-wb.jpgAnti-PSMA2 Rabbit Monoclonal AntibodyWestern blot analysis of PSMA2 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psma3-rabbit-monoclonal-antibody-m04355-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04355-psma3-primary-antibodies-wb-testing-1.jpgAnti-PSMA3 Rabbit Monoclonal AntibodyWestern blot analysis of PSMA3 using anti-PSMA3 antibody (M04355). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA3 antigen affinity purified monoclonal antibody (M04355) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA3 at approximately 28 kDa. The expected band size for PSMA3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04355-psma3-primary-antibodies-ihc-testing-1.jpgAnti-PSMA3 Rabbit Monoclonal AntibodyIHC analysis of PSMA3 using anti-PSMA3 antibody (M04355). PSMA3 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PSMA3 Antibody (M04355) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04355-psma3-primary-antibodies-ihc-testing-2.jpgAnti-PSMA3 Rabbit Monoclonal AntibodyIHC analysis of PSMA3 using anti-PSMA3 antibody (M04355). PSMA3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PSMA3 Antibody (M04355) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04355-psma3-primary-antibodies-ihc-testing-3.jpgAnti-PSMA3 Rabbit Monoclonal AntibodyIHC analysis of PSMA3 using anti-PSMA3 antibody (M04355). PSMA3 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PSMA3 Antibody (M04355) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamin-a-c-rabbit-monoclonal-antibody-m00438-5-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-5-lamina-c-primary-antibodies-wb-testing-1.jpgAnti-Lamin A/C Rabbit Monoclonal Antibody Western blot analysis of LaminA/C using anti-LaminA/C antibody (M00438-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LaminA/C antigen affinity purified monoclonal antibody (Catalog # M00438-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LaminA/C at approximately 70-74 kDa. The expected band size for LaminA/C is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-5-wb7.jpgAnti-Lamin A/C Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-5-icc1.jpgAnti-Lamin A/C Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-timm50-rabbit-monoclonal-antibody-m10056-1-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10056-1-wb.jpgAnti-TIMM50 Rabbit Monoclonal AntibodyWestern blot analysis of TIMM50 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bcl2l12-rabbit-monoclonal-antibody-m06508-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06508-wb.jpgAnti-BCL2L12 Rabbit Monoclonal AntibodyWestern blot analysis of BCL2L12 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-met-c-met-y1349-rabbit-monoclonal-antibody-p01488-1-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p01488-1-met-primary-antibodies-wb-testing-1.jpgAnti-Phospho-Met (c-Met) (Y1349) Rabbit Monoclonal Antibody Western blot analysis of Met (c-Met) using anti-Met (c-Met) antibody (P01488-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Met (c-Met) antigen affinity purified monoclonal antibody (Catalog # P01488-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Met (c-Met) at approximately 156 kDa. The expected band size for Met (c-Met) is at 156 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nek7-rabbit-monoclonal-antibody-m04996-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04996-wb7.jpgAnti-NEK7 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04996-wb8.jpgAnti-NEK7 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04996-wb.jpgAnti-NEK7 Rabbit Monoclonal AntibodyWestern blot analysis of NEK7 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nek6-rabbit-monoclonal-antibody-m03740-1-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03740-1-wb.jpgAnti-NEK6 Rabbit Monoclonal AntibodyWestern blot analysis of NEK6 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03740-1-wb7.jpgAnti-NEK6 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03740-1-wb8.jpgAnti-NEK6 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wapl-rabbit-monoclonal-antibody-m03684-boster.html2026-03-17T05:11:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03684-wb.jpgAnti-WAPL Rabbit Monoclonal AntibodyWestern blot analysis of WAPL expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-erbb3-y1222-rabbit-monoclonal-antibody-p00539-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00539-wb.jpgAnti-Phospho-ErbB3 (Y1222) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-ErbB3 (Y1222) expression in SKBR3 cell treated with neuregulin. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lta4h-rabbit-monoclonal-antibody-m02399-3-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02399-3-wb.jpgAnti-LTA4H Rabbit Monoclonal AntibodyWestern blot analysis of LTA4H expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tab3-rabbit-monoclonal-antibody-m05084-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05084-tab3-primary-antibodies-wb-testing-1.jpgAnti-TAB3 Rabbit Monoclonal Antibody Western blot analysis of TAB3 using anti-TAB3 antibody (M05084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: mouse Raw264.7 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB3 antigen affinity purified monoclonal antibody (Catalog # M05084) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB3 at approximately 90 kDa. The expected band size for TAB3 is at 79 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nfib-nf1b2-rabbit-monoclonal-antibody-m01537-2-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01537-2-wb7.jpgAnti-NFIB / NF1B2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01537-2-wb8.jpgAnti-NFIB / NF1B2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01537-2-wb.jpgAnti-NFIB / NF1B2 Rabbit Monoclonal AntibodyWestern blot analysis of NFIB / NF1B2 expression in NIH/3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01537-2-nfib-primary-antibodies-ihc-testing-1.jpgAnti-NFIB / NF1B2 Rabbit Monoclonal AntibodyIHC analysis of NFIB using anti-NFIB antibody (M01537-2). NFIB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NFIB Antibody (M01537-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01537-2-nfib-primary-antibodies-ihc-testing-2.jpgAnti-NFIB / NF1B2 Rabbit Monoclonal AntibodyIHC analysis of NFIB using anti-NFIB antibody (M01537-2). NFIB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NFIB Antibody (M01537-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01537-2-nfib-primary-antibodies-ihc-testing-3.jpgAnti-NFIB / NF1B2 Rabbit Monoclonal AntibodyIHC analysis of NFIB using anti-NFIB antibody (M01537-2). NFIB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NFIB Antibody (M01537-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01537-2-nfib-primary-antibodies-ihc-testing-4.jpgAnti-NFIB / NF1B2 Rabbit Monoclonal AntibodyIHC analysis of NFIB using anti-NFIB antibody (M01537-2). NFIB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NFIB Antibody (M01537-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-copa-rabbit-monoclonal-antibody-m03987-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03987-wb.jpgAnti-COPA Rabbit Monoclonal AntibodyWestern blot analysis of COPA expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nogo-receptor-rabbit-monoclonal-antibody-m02250-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02250-rtn4r-primary-antibodies-wb-testing-1.jpgAnti-Nogo Receptor Rabbit Monoclonal Antibody Western blot analysis of Nogo Receptor using anti-Nogo Receptor antibody (M02250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo Receptor antigen affinity purified monoclonal antibody (Catalog # M02250) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo Receptor at approximately 70 kDa. The expected band size for Nogo Receptor is at 51,70 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ror-beta-rabbit-monoclonal-antibody-m09711-1-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09711-1-wb.jpgAnti-ROR beta Rabbit Monoclonal AntibodyWestern blot analysis of ROR beta expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09711-1-wb7.jpgAnti-ROR beta Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mov10-rabbit-monoclonal-antibody-m04952-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04952-wb7.jpgAnti-Mov10 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04952-wb.jpgAnti-Mov10 Rabbit Monoclonal AntibodyWestern blot analysis of Mov10 expression in (1) 293 cell lysate; (2) NIH/3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04952-wb8.jpgAnti-Mov10 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04952-wb9.jpgAnti-Mov10 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-igfbp1-rabbit-monoclonal-antibody-m00922-1-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00922-1-wb7.jpgAnti-IGFBP1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00922-1-wb8.jpgAnti-IGFBP1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00922-1-wb.jpgAnti-IGFBP1 Rabbit Monoclonal AntibodyWestern blot analysis of IGFBP1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcak-rabbit-monoclonal-antibody-m03202-1-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03202-1-mcak-primary-antibodies-wb-testing-1.jpgAnti-MCAK Rabbit Monoclonal Antibody Western blot analysis of MCAK/KIF2C using anti-MCAK/KIF2C antibody (M03202-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCAK/KIF2C antigen affinity purified monoclonal antibody (M03202-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCAK/KIF2C at approximately 81 kDa. The expected band size for MCAK/KIF2C is at 81 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-casein-rabbit-monoclonal-antibody-m07562-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07562-wb.jpgAnti-Casein Rabbit Monoclonal AntibodyWestern blot analysis of Casein expression in Human milk lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il7r-alpha-cd127-rabbit-monoclonal-antibody-m02222-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02222-il7r-primary-antibodies-wb-testing-1.jpgAnti-IL7R alpha/CD127 Rabbit Monoclonal AntibodyFigure 1. Western blot analysis of CD127/IL7R using anti-CD127/IL7R antibody (M02222). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD127/IL7R antigen affinity purified monoclonal antibody (M02222) at a dilution of 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD127/IL7R at approximately 75 kDa. The expected band size for CD127/IL7R is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02222-il7r-primary-antibodies-ihc-testing-2.jpgAnti-IL7R alpha/CD127 Rabbit Monoclonal AntibodyFigure 2. IHC analysis of CD127/IL7R using anti-CD127/IL7R antibody (M02222). CD127/IL7R was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-CD127/IL7R Antibody (M02222) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-za2g-azgp1-rabbit-monoclonal-antibody-m02718-1-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02718-1-wb.jpgAnti-ZA2G / AZGP1 Rabbit Monoclonal AntibodyWestern blot analysis of ZA2G / AZGP1 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02718-1-azgp1-primary-antibodies-ihc-testing-1.jpgAnti-ZA2G / AZGP1 Rabbit Monoclonal AntibodyIHC analysis of ZAG/AZGP1 using anti-ZAG/AZGP1 antibody (M02718-1). ZAG/AZGP1 was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ZAG/AZGP1 Antibody (M02718-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-scramblase-1-rabbit-monoclonal-antibody-m02995-1-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02995-1-wb.jpgAnti-Scramblase 1 Rabbit Monoclonal AntibodyWestern blot analysis of Scramblase 1 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calpain-s1-rabbit-monoclonal-antibody-m04111-1-boster.html2026-03-17T05:11:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04111-1-wb.jpgAnti-Calpain S1 Rabbit Monoclonal AntibodyWestern blot analysis of Calpain S1 expression in T47D cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04111-1-capns1-primary-antibodies-ihc-testing-1_1.jpgAnti-Calpain S1 Rabbit Monoclonal AntibodyIHC analysis of CAPNS1 using anti-CAPNS1 antibody (M04111-1). CAPNS1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CAPNS1 Antibody (M04111-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04111-1-capns1-primary-antibodies-ihc-testing-2_1.jpgAnti-Calpain S1 Rabbit Monoclonal AntibodyIHC analysis of CAPNS1 using anti-CAPNS1 antibody (M04111-1). CAPNS1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CAPNS1 Antibody (M04111-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-peroxiredoxin-5-rabbit-monoclonal-antibody-m02891-1-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02891-1-prdx5-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 5 Rabbit Monoclonal Antibody Western blot analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (M02891-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 5 antigen affinity purified monoclonal antibody (Catalog # M02891-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa. The expected band size for Peroxiredoxin 5 is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbx2-rabbit-monoclonal-antibody-m06356-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06356-cbx2-primary-antibodies-wb-testing-1.jpgAnti-CBX2 Rabbit Monoclonal Antibody Western blot analysis of CBX2 using anti-CBX2 antibody (M06356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX2 antigen affinity purified monoclonal antibody (Catalog # M06356) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBX2 at approximately 60 kDa. The expected band size for CBX2 is at 56,60 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc22a1-rabbit-monoclonal-antibody-m01945-1-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01945-1-wb.jpgAnti-SLC22A1 Rabbit Monoclonal AntibodyWestern blot analysis of SLC22A1 expression in SW480 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phapi2-april-rabbit-monoclonal-antibody-m05822-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05822-anp32b-primary-antibodies-wb-testing-1.jpgAnti-PHAPI2 / APRIL Rabbit Monoclonal AntibodyWestern blot analysis of ANP32B using anti-ANP32B antibody (M05822). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANP32B antigen affinity purified monoclonal antibody (M05822) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ANP32B at approximately 29-32 kDa. The expected band size for ANP32B is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-disc1-rabbit-monoclonal-antibody-m00750-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00750-wb.jpgAnti-DISC1 Rabbit Monoclonal AntibodyWestern blot analysis of DISC1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate; (3) PC-12 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcf21-rabbit-monoclonal-antibody-m05375-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05375-wb.jpgAnti-TCF21 Rabbit Monoclonal AntibodyWestern blot analysis of TCF21 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-g3bp-rabbit-monoclonal-antibody-m02199-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02199-g3bp-primary-antibodies-wb-testing-1.jpgAnti-G3BP Rabbit Monoclonal AntibodyWestern blot analysis of G3BP using anti-G3BP antibody (M02199). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G3BP antigen affinity purified monoclonal antibody (M02199) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for G3BP at approximately 68 kDa. The expected band size for G3BP is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02199-g3bp-primary-antibodies-ihc-testing-1.jpgAnti-G3BP Rabbit Monoclonal AntibodyIHC analysis of G3BP using anti-G3BP antibody (M02199). G3BP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-G3BP Antibody (M02199) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02199-g3bp-primary-antibodies-ihc-testing-2.jpgAnti-G3BP Rabbit Monoclonal AntibodyIHC analysis of G3BP using anti-G3BP antibody (M02199). G3BP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-G3BP Antibody (M02199) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02199-g3bp-primary-antibodies-ihc-testing-3.jpgAnti-G3BP Rabbit Monoclonal AntibodyIHC analysis of G3BP using anti-G3BP antibody (M02199). G3BP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-G3BP Antibody (M02199) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02199-g3bp1-primary-antibodies-if-testing-2.jpgAnti-G3BP Rabbit Monoclonal AntibodyImmunocytochemistry analysis of N2A cells, using G3BP antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neuronatin-rabbit-monoclonal-antibody-m08311-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08311-wb.jpgAnti-Neuronatin Rabbit Monoclonal AntibodyWestern blot analysis of Neuronatin expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bmf-rabbit-monoclonal-antibody-m02432-1-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02432-1-wb.jpgAnti-Bmf Rabbit Monoclonal AntibodyWestern blot analysis of Bmf expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vps11-rabbit-monoclonal-antibody-m05575-boster.html2026-03-17T05:11:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05575-wb.jpgAnti-VPS11 Rabbit Monoclonal AntibodyWestern blot analysis of VPS11 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-y14-rabbit-monoclonal-antibody-m02769-1-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02769-1-wb.jpgAnti-Y14 Rabbit Monoclonal AntibodyWestern blot analysis of Y14 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-abce1-rabbit-monoclonal-antibody-m04173-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04173-wb.jpgAnti-ABCE1 Rabbit Monoclonal AntibodyWestern blot analysis of ABCE1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ent2-rabbit-monoclonal-antibody-m04718-1-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04718-1-wb7.jpgAnti-ENT2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04718-1-wb.jpgAnti-ENT2 Rabbit Monoclonal AntibodyWestern blot analysis of ENT2 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyclophilin-40-rabbit-monoclonal-antibody-m02424-2-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02424-2-wb.jpgAnti-Cyclophilin 40 Rabbit Monoclonal AntibodyWestern blot analysis of Cyclophilin 40 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rap1a-rap1b-rabbit-monoclonal-antibody-m01848-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01848-rap1a-rap1b-primary-antibodies-wb-testing-1.jpgAnti-RAP1A + RAP1B Rabbit Monoclonal AntibodyWestern blot analysis of RAP1A,B using anti-RAP1A,B antibody (M01848). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A,B antigen affinity purified monoclonal antibody (M01848) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAP1A,B at approximately 19 kDa. The expected band size for RAP1A,B is at 21 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vps18-rabbit-monoclonal-antibody-m06047-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06047-wb.jpgAnti-VPS18 Rabbit Monoclonal AntibodyWestern blot analysis of VPS18 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-polr2c-rabbit-monoclonal-antibody-m09308-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09308-wb.jpgAnti-POLR2C Rabbit Monoclonal AntibodyWestern blot analysis of POLR2C expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mad1-rabbit-monoclonal-antibody-m01137-1-boster.html2026-03-17T05:11:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01137-1-wb.jpgAnti-MAD1 Rabbit Monoclonal AntibodyWestern blot analysis of MAD1 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cystathionase-rabbit-monoclonal-antibody-m01803-1-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01803-1-wb.jpgAnti-Cystathionase Rabbit Monoclonal AntibodyWestern blot analysis of Cystathionase expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myl9-rabbit-monoclonal-antibody-m06446-1-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-1-wb7.jpgAnti-MYL9 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-1-wb10.jpgAnti-MYL9 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-1-wb8.jpgAnti-MYL9 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-1-wb.jpgAnti-MYL9 Rabbit Monoclonal AntibodyWestern blot analysis of MYL9 expression in Human uterus lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-1-wb9.jpgAnti-MYL9 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-par2-rabbit-monoclonal-antibody-m01081-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-wb-testing-1.jpgAnti-PAR2 Rabbit Monoclonal Antibody Western blot analysis of PAR2 using anti-PAR2 antibody (M01081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR2 antigen affinity purified polyclonal antibody (Catalog # M01081) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR2 at approximately 50 kDa. The expected band size for PAR2 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-2.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-3.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-4.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-5.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-6.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-7.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-8.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-9.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-10.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-11.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-12.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-13.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-14.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-15.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-16.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01081-par2-primary-antibodies-ihc-testing-17.jpgAnti-PAR2 Rabbit Monoclonal Antibody IHC analysis of PAR2 using anti-PAR2 antibody (M01081). PAR2 was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAR2 Antibody (M01081) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mag-rabbit-monoclonal-antibody-m03019-1-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03019-1-wb.jpgAnti-MAG Rabbit Monoclonal AntibodyWestern blot analysis of MAG expression in Rat brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aha1-rabbit-monoclonal-antibody-m05733-2-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05733-2-wb.jpgAnti-AHA1 Rabbit Monoclonal AntibodyWestern blot analysis of AHA1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp1a3-rabbit-monoclonal-antibody-m02278-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02278-atp1a3-primary-antibodies-wb-testing-1.jpgAnti-ATP1A3 Rabbit Monoclonal Antibody Western blot analysis of ATP1A3 using anti-ATP1A3 antibody (M02278). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A3 antigen affinity purified monoclonal antibody (Catalog # M02278) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A3 at approximately 112 kDa. The expected band size for ATP1A3 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02278-wb7.jpgAnti-ATP1A3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02278-wb.jpgAnti-ATP1A3 Rabbit Monoclonal AntibodyWestern blot analysis of ATP1A3 expression in Rat brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdkn2c-rabbit-monoclonal-antibody-m03299-1-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03299-1-cdkn2c-primary-antibodies-wb-testing-1.jpgAnti-CDKN2C Rabbit Monoclonal Antibody Western blot analysis of CDKN2C using anti-CDKN2C antibody (M03299-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDKN2C antigen affinity purified monoclonal antibody (M03299-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDKN2C at approximately 18 kDa. The expected band size for CDKN2C is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rea-rabbit-monoclonal-antibody-m03315-1-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03315-1-wb.jpgAnti-REA Rabbit Monoclonal AntibodyWestern blot analysis of REA expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mt-nd1-rabbit-monoclonal-antibody-m02292-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02292-wb.jpgAnti-MT-ND1 Rabbit Monoclonal AntibodyWestern blot analysis of MT-ND1 expression in Human fetal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crabp1-rabbit-monoclonal-antibody-m07951-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07951-wb.jpgAnti-CRABP1 Rabbit Monoclonal AntibodyWestern blot analysis of CRABP1 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccl19-rabbit-monoclonal-antibody-m01605-2-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01605-2-wb.jpgAnti-CCL19 Rabbit Monoclonal AntibodyWestern blot analysis of CCL19 expression in Human Macrophage Inflammatory Protein 3 beta recombinant protein lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-legumain-rabbit-monoclonal-antibody-m06476-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06476-legumain-primary-antibodies-wb-testing-1.jpgAnti-Legumain Rabbit Monoclonal AntibodyWestern blot analysis of Legumain using anti-Legumain antibody (M06476). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Legumain antigen affinity purified monoclonal antibody (M06476) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Legumain at approximately 36,46,56 kDa. The expected band size for Legumain is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hltf-rabbit-monoclonal-antibody-m03682-boster.html2026-03-17T05:12:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03682-wb.jpgAnti-HLTF Rabbit Monoclonal AntibodyWestern blot analysis of HLTF expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uap56-rabbit-monoclonal-antibody-m03490-3-boster.html2026-03-17T05:12:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03490-3-wb.jpgAnti-UAP56 Rabbit Monoclonal AntibodyWestern blot analysis of UAP56 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-renalase-rabbit-monoclonal-antibody-m06291-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06291-wb.jpgAnti-Renalase Rabbit Monoclonal AntibodyWestern blot analysis of Renalase expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06291-renalase-prmary-antibodies-icc-testing-1.jpgAnti-Renalase Rabbit Monoclonal AntibodyICC/IF analysis of Renalase using anti-Renalase antibody (M06291). Renalase was detected in an immunocytochemical section of mouse Beta-TC6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with rabbit anti-Renalase Antibody (M06291) at a dilution of 1:50 overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-capg-rabbit-monoclonal-antibody-m04512-boster.html2026-03-17T05:12:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04512-wb.jpgAnti-CAPG Rabbit Monoclonal AntibodyWestern blot analysis of CAPG expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dlat-rabbit-monoclonal-antibody-m04469-1-boster.html2026-03-17T05:12:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04469-1-wb7.jpgAnti-DLAT Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04469-1-wb.jpgAnti-DLAT Rabbit Monoclonal AntibodyWestern blot analysis of DLAT expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04469-1-wb8.jpgAnti-DLAT Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04469-1-dlat-primary-antibodies-ihc-testing-4.jpgAnti-DLAT Rabbit Monoclonal Antibody IHC analysis of DLAT using anti-DLAT antibody (M04469-1). DLAT was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DLAT Antibody (M04469-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04469-1-icc2.jpgAnti-DLAT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04469-1-icc1.jpgAnti-DLAT Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-idh1-rabbit-monoclonal-antibody-m00129-2-boster.html2026-03-17T05:12:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00129-2-idh1-primary-antibodies-ihc-testing-1.jpgAnti-IDH1 Rabbit Monoclonal AntibodyIHC analysis of IDH1 using anti-IDH1 antibody (M00129-2). IDH1 was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IDH1 Antibody (M00129-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00129-2-idh1-primary-antibodies-wb-testing-1.jpgAnti-IDH1 Rabbit Monoclonal AntibodyWestern blot analysis of IDH1 using anti-IDH1 antibody (M00129-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human LNCAP whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH1 antigen affinity purified monoclonal antibody (M00129-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IDH1 at approximately 47 kDa. The expected band size for IDH1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dgat1-rabbit-monoclonal-antibody-m02522-boster.html2026-03-17T05:12:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02522-dgat1-primary-antibodies-wb-testing-1.jpgAnti-DGAT1 Rabbit Monoclonal AntibodyWestern blot analysis of DGAT1 using anti-DGAT1 antibody (M02522). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1 antigen affinity purified monoclonal antibody (M02522) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DGAT1 at approximately 50 kDa. The expected band size for DGAT1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02522-dgat1-primary-antibodies-ihc-testing-1.jpgAnti-DGAT1 Rabbit Monoclonal AntibodyIHC analysis of DGAT1 using anti-DGAT1 antibody (M02522). DGAT1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DGAT1 Antibody (M02522) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c-maf-rabbit-monoclonal-antibody-m00654-2-boster.html2026-03-17T05:12:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00654-2-maf-primary-antibodies-wb-testing-1.jpgAnti-c-Maf Rabbit Monoclonal AntibodyWestern blot analysis of MAF using anti-MAF antibody (M00654-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat eye tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAF antigen affinity purified monoclonal antibody (M00654-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAF at approximately 44 kDa. The expected band size for MAF is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-carboxypeptidase-a1-a2-b-rabbit-monoclonal-antibody-m05985-2-boster.html2026-03-17T05:12:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05985-2-wb.jpgAnti-Carboxypeptidase A1+A2+B Rabbit Monoclonal AntibodyWestern blot analysis of Carboxypeptidase A1+A2+B expression in Mouse pancreas lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-per2-rabbit-monoclonal-antibody-m01075-boster.html2026-03-17T05:12:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01075-wb7.jpgAnti-PER2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01075-wb.jpgAnti-PER2 Rabbit Monoclonal AntibodyWestern blot analysis of PER2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddx17-rabbit-monoclonal-antibody-m01656-boster.html2026-03-17T05:12:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01656-ddx17-primary-antibodies-wb-testing-1.jpgAnti-DDX17 Rabbit Monoclonal AntibodyWestern blot analysis of DDX17 using anti-DDX17 antibody (M01656). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX17 antigen affinity purified monoclonal antibody (M01656) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX17 at approximately 70 kDa. The expected band size for DDX17 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01656-ddx17-primary-antibodies-ihc-testing-1.jpgAnti-DDX17 Rabbit Monoclonal AntibodyIHC analysis of DDX17 using anti-DDX17 antibody (M01656). DDX17 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DDX17 Antibody (M01656) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01656-ddx17-primary-antibodies-ihc-testing-2.jpgAnti-DDX17 Rabbit Monoclonal AntibodyIHC analysis of DDX17 using anti-DDX17 antibody (M01656). DDX17 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DDX17 Antibody (M01656) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01656-ddx17-primary-antibodies-ihc-testing-3.jpgAnti-DDX17 Rabbit Monoclonal AntibodyIHC analysis of DDX17 using anti-DDX17 antibody (M01656). DDX17 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DDX17 Antibody (M01656) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbx4-rabbit-monoclonal-antibody-m03388-boster.html2026-03-17T05:12:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03388-cbx4-primary-antibodies-wb-testing-1.jpgAnti-CBX4 Rabbit Monoclonal AntibodyWestern blot analysis of CBX4 using anti-CBX4 antibody (M03388). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX4 antigen affinity purified monoclonal antibody (M03388) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CBX4 at approximately 50 kDa. The expected band size for CBX4 is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nap1l1-rabbit-monoclonal-antibody-m05100-boster.html2026-03-17T05:12:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05100-wb.jpgAnti-NAP1L1 Rabbit Monoclonal AntibodyWestern blot analysis of NAP1L1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-amhr2-rabbit-monoclonal-antibody-m03970-boster.html2026-03-17T05:12:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03970-wb.jpgAnti-AMHR2 Rabbit Monoclonal AntibodyWestern blot analysis of AMHR2 expression in SK-OV-3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cryaa-rabbit-monoclonal-antibody-m01900-4-boster.html2026-03-17T05:12:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01900-4-wb.jpgAnti-CRYAA Rabbit Monoclonal AntibodyWestern blot analysis of CRYAA expression in Mouse eyeball lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01900-4-wb7.jpgAnti-CRYAA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crmp5-rabbit-monoclonal-antibody-m08644-3-boster.html2026-03-17T05:12:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08644-3-wb.jpgAnti-CRMP5 Rabbit Monoclonal AntibodyWestern blot analysis of CRMP5 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp1b1-rabbit-monoclonal-antibody-m03469-boster.html2026-03-17T05:12:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03469-wb.jpgAnti-ATP1B1 Rabbit Monoclonal AntibodyWestern blot analysis of ATP1B1 expression in Mouse brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zbtb7a-rabbit-monoclonal-antibody-m03081-boster.html2026-03-17T05:12:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03081-zbtb7a-primary-antibodies-wb-testing-1.jpgAnti-ZBTB7A Rabbit Monoclonal Antibody Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (M03081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified monoclonal antibody (Catalog # M03081) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 75 kDa. The expected band size for ZBTB7A is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-txnrd2-rabbit-monoclonal-antibody-m03900-1-boster.html2026-03-17T05:12:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03900-1-wb.jpgAnti-TXNRD2 Rabbit Monoclonal AntibodyWestern blot analysis of TXNRD2 expression in LnCap cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mdh1-rabbit-monoclonal-antibody-m04262-boster.html2026-03-17T05:12:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04262-wb.jpgAnti-MDH1 Rabbit Monoclonal AntibodyWestern blot analysis of MDH1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-paf1-rabbit-monoclonal-antibody-m01640-boster.html2026-03-17T05:12:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01640-wb.jpgAnti-PAF1 Rabbit Monoclonal AntibodyWestern blot analysis of PAF1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ebp1-pa2g4-rabbit-monoclonal-antibody-m02791-1-boster.html2026-03-24T05:36:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02791-1-pa2g4-primary-antibodies-wb-testing-1.jpgAnti-EBP1 / PA2G4 Rabbit Monoclonal AntibodyWestern blot analysis of PA2G4 using anti-PA2G4 antibody (M02791-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PA2G4 antigen affinity purified monoclonal antibody (M02791-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PA2G4 at approximately 44 kDa. The expected band size for PA2G4 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02791-1-pa2g4-primary-antibodies-ihc-testing-1.jpgAnti-EBP1 / PA2G4 Rabbit Monoclonal AntibodyIHC analysis of PA2G4 using anti-PA2G4 antibody (M02791-1). PA2G4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PA2G4 Antibody (M02791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02791-1-pa2g4-primary-antibodies-ihc-testing-2.jpgAnti-EBP1 / PA2G4 Rabbit Monoclonal AntibodyIHC analysis of PA2G4 using anti-PA2G4 antibody (M02791-1). PA2G4 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PA2G4 Antibody (M02791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02791-1-pa2g4-primary-antibodies-ihc-testing-3.jpgAnti-EBP1 / PA2G4 Rabbit Monoclonal AntibodyIHC analysis of PA2G4 using anti-PA2G4 antibody (M02791-1). PA2G4 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PA2G4 Antibody (M02791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-traf6bp-rabbit-monoclonal-antibody-m04336-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04336-tax1bp1-primary-antibodies-wb-testing-1.jpgAnti-TRAF6BP Rabbit Monoclonal Antibody Western blot analysis of TRAF6BP using anti-TRAF6BP antibody (M04336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6BP antigen affinity purified monoclonal antibody (Catalog # M04336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6BP at approximately 91 kDa. The expected band size for TRAF6BP is at 91 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-nak-tbk1-s172-rabbit-monoclonal-antibody-p00261-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00261-fcimb-14-1393680-g003.jpgAnti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal AntibodyEV71 infection inhibits the TLR4/MYD88/NF-κB and TBK1 pathway. (A) The network of TLR4 interacting proteins; (B) MYD88 gene expression of the GSE15323 dataset from the GEO database; (C–F) Western blot analysis of MYD88, p-NF-κB p65, NF-κB p65 and VP1 protein expression in EV71 infected RD cells at different MOIs. (G–I) Western blot analysis of p-TBK1/TBK1 and VP1 protein expression in EV71 infected RD cells at different MOIs. Data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1393680/full'>38938877</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00261-tbk1-primary-antibodies-wb-testing-1_1.jpgAnti-Phospho-NAK/TBK1 (S172) Rabbit Monoclonal Antibody Western blot analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (P00261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAK/TBK1 antigen affinity purified monoclonal antibody (Catalog # P00261) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAK/TBK1 at approximately 84 kDa. The expected band size for NAK/TBK1 is at 84 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-galactosidase-alpha-rabbit-monoclonal-antibody-m01135-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01135-wb.jpgAnti-Galactosidase alpha Rabbit Monoclonal AntibodyWestern blot analysis of Galactosidase alpha expression in MCF-7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vav3-rabbit-monoclonal-antibody-m03204-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03204-vav3-primary-antibodies-wb-testing-1.jpgAnti-VAV3 Rabbit Monoclonal Antibody Western blot analysis of VAV3 using anti-VAV3 antibody (M03204). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAV3 antigen affinity purified monoclonal antibody (M03204) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VAV3 at approximately 98 kDa. The expected band size for VAV3 is at 98 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ror2-rabbit-monoclonal-antibody-m01840-1-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01840-1-ror2-primary-antibodies-wb-testing-1.jpgAnti-ROR2 Rabbit Monoclonal Antibody Western blot analysis of ROR2 using anti-ROR2 antibody (M01840-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR2 antigen affinity purified monoclonal antibody (M01840-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR2 at approximately 100 kDa. The expected band size for ROR2 is at 105 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prkra-rabbit-monoclonal-antibody-m02744-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02744-wb.jpgAnti-PRKRA Rabbit Monoclonal AntibodyWestern blot analysis of PRKRA expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hamartin-rabbit-monoclonal-antibody-m00365-1-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00365-1-wb.jpgAnti-Hamartin Rabbit Monoclonal AntibodyWestern blot analysis of Hamartin expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmprss2-rabbit-monoclonal-antibody-m00666-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00666-tmprss2-primary-antibodies-wb-testing-1_1.jpgAnti-TMPRSS2 Rabbit Monoclonal Antibody Western blot analysis of TMPRSS2 using anti-TMPRSS2 antibody (M00666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS2 antigen affinity purified monoclonal antibody (Catalog # M00666) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS2 at approximately 54 kDa. The expected band size for TMPRSS2 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00666-tmprss2-primary-antibodies-fcm-testing-2_1.jpgAnti-TMPRSS2 Rabbit Monoclonal Antibody Flow Cytometry analysis of U2OS cells using anti-TMPRSS2 antibody (M00666). Overlay histogram showing U2OS cells stained with M00666 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TMPRSS2 Antibody (M00666) at 1:60 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) at 1:60 was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:60 used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00666-wb7.jpgAnti-TMPRSS2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00666-wb10.jpgAnti-TMPRSS2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cad-rabbit-monoclonal-antibody-m00463-1-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00463-1-wb.jpgAnti-CAD Rabbit Monoclonal AntibodyWestern blot analysis of CAD expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neurogenin-2-rabbit-monoclonal-antibody-m10153-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10153-wb7.jpgAnti-Neurogenin 2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10153-wb.jpgAnti-Neurogenin 2 Rabbit Monoclonal AntibodyWestern blot analysis of Neurogenin 2 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atgl-pnpla2-rabbit-monoclonal-antibody-m01800-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01800-atgl-primary-antibodies-wb-testing-1.jpgAnti-ATGL / PNPLA2 Rabbit Monoclonal Antibody Western blot analysis of ATGL/PNPLA2 using anti-ATGL/PNPLA2 antibody (M01800). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATGL/PNPLA2 antigen affinity purified monoclonal antibody (Catalog # M01800) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATGL/PNPLA2 at approximately 55 kDa. The expected band size for ATGL/PNPLA2 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gpcr-lgr6-rabbit-monoclonal-antibody-m05990-1-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05990-1-wb.jpgAnti-GPCR / LGR6 Rabbit Monoclonal AntibodyWestern blot analysis of GPCR / LGR6 expression in HUVEC cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acvr1b-rabbit-monoclonal-antibody-m02882-boster.html2026-03-17T05:12:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02882-wb7.jpgAnti-ACVR1B Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02882-wb8.jpgAnti-ACVR1B Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02882-wb.jpgAnti-ACVR1B Rabbit Monoclonal AntibodyWestern blot analysis of ACVR1B expression in U87-MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-icos-rabbit-monoclonal-antibody-m00291-1-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00291-1-icos-primary-antibodies-wb-testing-1.jpgAnti-ICOS Rabbit Monoclonal Antibody Western blot analysis of ICOS using anti-ICOS antibody (M00291-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICOS antigen affinity purified monoclonal antibody (M00291-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICOS at approximately 23 kDa. The expected band size for ICOS is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ldhb-rabbit-monoclonal-antibody-m02552-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02552-wb7.jpgAnti-LDHB Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02552-wb8.jpgAnti-LDHB Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02552-wb.jpgAnti-LDHB Rabbit Monoclonal AntibodyWestern blot analysis of LDHB expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kdm4b-jmjd2b-rabbit-monoclonal-antibody-m04127-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04127-kdm4b-primary-antibodies-wb-testing-1.jpgAnti-KDM4B / JMJD2B Rabbit Monoclonal AntibodyWestern blot analysis of KDM4B using anti-KDM4B antibody (M04127). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW480 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM4B antigen affinity purified monoclonal antibody (M04127) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KDM4B at approximately 150 kDa. The expected band size for KDM4B is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04127-kdm4b-primary-antibodies-ihc-testing-1.jpgAnti-KDM4B / JMJD2B Rabbit Monoclonal AntibodyIHC analysis of KDM4B using anti-KDM4B antibody (M04127). KDM4B was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-KDM4B Antibody (M04127) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wasp-rabbit-monoclonal-antibody-m10788-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10788-wasp-primary-antibodies-wb-testing-1.jpgAnti-WASP Rabbit Monoclonal Antibody Western blot analysis of WASP using anti-WASP antibody (M10788). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WASP antigen affinity purified monoclonal antibody (Catalog # M10788) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WASP at approximately 60 kDa. The expected band size for WASP is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-angiotensinogen-rabbit-monoclonal-antibody-m02103-2-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02103-2-wb.jpgAnti-Angiotensinogen Rabbit Monoclonal AntibodyWestern blot analysis of Angiotensinogen expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fibrinogen-alpha-chain-rabbit-monoclonal-antibody-m00816-1-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00816-1-fga-primary-antibodies-wb-testing-1.jpgAnti-Fibrinogen alpha chain Rabbit Monoclonal Antibody Western blot analysis of Fibrinogen Alpha Chain using anti-Fibrinogen Alpha Chain antibody (M00816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrinogen Alpha Chain antigen affinity purified monoclonal antibody (Catalog # M00816-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrinogen Alpha Chain at approximately 95 kDa. The expected band size for Fibrinogen Alpha Chain is at 95 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kaiso-rabbit-monoclonal-antibody-m04754-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04754-wb.jpgAnti-KAISO Rabbit Monoclonal AntibodyWestern blot analysis of KAISO expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp28-rabbit-monoclonal-antibody-m05040-1-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05040-1-wb.jpgAnti-USP28 Rabbit Monoclonal AntibodyWestern blot analysis of USP28 expression in SW480 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bhmt-rabbit-monoclonal-antibody-m06712-2-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06712-2-wb.jpgAnti-BHMT Rabbit Monoclonal AntibodyWestern blot analysis of BHMT expression in Human fetal kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trim29-rabbit-monoclonal-antibody-m04472-2-boster.html2026-03-17T05:12:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04472-2-wb.jpgAnti-TRIM29 Rabbit Monoclonal AntibodyWestern blot analysis of TRIM29 expression in JAR cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-poliovirus-receptor-rabbit-monoclonal-antibody-m00664-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-pvr-primary-antibodies-wb-testing-1.jpgAnti-Poliovirus Receptor Rabbit Monoclonal AntibodyWestern blot analysis of CD155/PVR using anti-CD155/PVR antibody (M00664). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD155/PVR antigen affinity purified monoclonal antibody (M00664) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD155/PVR at approximately 70-80 kDa. The expected band size for CD155/PVR is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-1-spectrin-rabbit-monoclonal-antibody-m03376-1-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03376-1-wb.jpgAnti-alpha 1 Spectrin Rabbit Monoclonal AntibodyWestern blot analysis of alpha 1 Spectrin expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-salivary-alpha-amylase-rabbit-monoclonal-antibody-m12967-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12967-wb.jpgAnti-Salivary alpha amylase Rabbit Monoclonal AntibodyWestern blot analysis of Salivary alpha amylase expression in Human pancreas lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccl4-mip1-beta-rabbit-monoclonal-antibody-m00703-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00703-wb.jpgAnti-CCL4/MIP1 beta Rabbit Monoclonal AntibodyWestern blot analysis of CCL4/MIP1 beta expression in (1) THP-1 cell lysate; (2) THP-1 cell treated with PMA+LPS+Brefeldin A. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bcar1-rabbit-monoclonal-antibody-m00960-2-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00960-2-wb.jpgAnti-BCAR1 Rabbit Monoclonal AntibodyWestern blot analysis of BCAR1 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hoxa13-rabbit-monoclonal-antibody-m03305-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03305-wb.jpgAnti-HOXA13 Rabbit Monoclonal AntibodyWestern blot analysis of HOXA13 expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03305-wb7.jpgAnti-HOXA13 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03305-wb8.jpgAnti-HOXA13 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vps24-rabbit-monoclonal-antibody-m04800-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04800-wb.jpgAnti-VPS24 Rabbit Monoclonal AntibodyWestern blot analysis of VPS24 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fetal-hemoglobin-rabbit-monoclonal-antibody-m02830-2-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02830-2-wb.jpgAnti-fetal hemoglobin Rabbit Monoclonal AntibodyWestern blot analysis of fetal hemoglobin expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-triosephosphate-isomerase-rabbit-monoclonal-antibody-m02559-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02559-wb.jpgAnti-Triosephosphate isomerase Rabbit Monoclonal AntibodyWestern blot analysis of Triosephosphate isomerase expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-limpii-rabbit-monoclonal-antibody-m05090-boster.html2026-03-17T05:12:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05090-wb.jpgAnti-LIMPII Rabbit Monoclonal AntibodyWestern blot analysis of LIMPII expression in (1) HeLa cell lysate; (2) Mouse liver lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-magoh-rabbit-monoclonal-antibody-m04427-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04427-wb.jpgAnti-MAGOH Rabbit Monoclonal AntibodyWestern blot analysis of MAGOH expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ass1-rabbit-monoclonal-antibody-m02212-3-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02212-3-ass1-primary-antibodies-wb-testing-1.jpgAnti-ASS1 Rabbit Monoclonal Antibody Western blot analysis of ASS1 using anti-ASS1 antibody (M02212-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASS1 antigen affinity purified monoclonal antibody (M02212-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ASS1 at approximately 45 kDa. The expected band size for ASS1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klrg1-rabbit-monoclonal-antibody-m02084-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02084-klrg1-primary-antibodies-wb-testing-1.jpgAnti-KLRG1 Rabbit Monoclonal AntibodyWestern blot analysis of KLRG1 using anti-KLRG1 antibody (M02084). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLRG1 antigen affinity purified monoclonal antibody (M02084) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KLRG1 at approximately 38 kDa. The expected band size for KLRG1 is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bach1-brip1-rabbit-monoclonal-antibody-m01995-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01995-wb.jpgAnti-BACH1/BRIP1 Rabbit Monoclonal AntibodyWestern blot analysis of BACH1/BRIP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smchd1-rabbit-monoclonal-antibody-m04751-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04751-smchd1-primary-antibodies-wb-testing-1.jpgAnti-SMCHD1 Rabbit Monoclonal AntibodyWestern blot analysis of SMCHD1 using anti-SMCHD1 antibody (M04751). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMCHD1 antigen affinity purified monoclonal antibody (M04751) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMCHD1 at approximately 226 kDa. The expected band size for SMCHD1 is at 226 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thra-rabbit-monoclonal-antibody-m01775-1-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01775-1-wb.jpgAnti-THRA Rabbit Monoclonal AntibodyWestern blot analysis of THRA expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ncf1-rabbit-monoclonal-antibody-m01586-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01586-wb.jpgAnti-NCF1 Rabbit Monoclonal AntibodyWestern blot analysis of NCF1 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calmegin-rabbit-monoclonal-antibody-m05261-1-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05261-1-wb.jpgAnti-Calmegin Rabbit Monoclonal AntibodyWestern blot analysis of Calmegin expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crkl-rabbit-monoclonal-antibody-m02100-1-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02100-1-wb.jpgAnti-CRKL Rabbit Monoclonal AntibodyWestern blot analysis of CRKL expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fe65-rabbit-monoclonal-antibody-m03090-1-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03090-1-wb.jpgAnti-FE65 Rabbit Monoclonal AntibodyWestern blot analysis of FE65 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apom-rabbit-monoclonal-antibody-m02451-2-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02451-2-wb.jpgAnti-ApoM Rabbit Monoclonal AntibodyWestern blot analysis of ApoM expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdzk1-rabbit-monoclonal-antibody-m03176-1-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03176-1-wb.jpgAnti-PDZK1 Rabbit Monoclonal AntibodyWestern blot analysis of PDZK1 expression in T47-D cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apod-rabbit-monoclonal-antibody-m02196-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02196-wb.jpgAnti-ApoD Rabbit Monoclonal AntibodyWestern blot analysis of ApoD expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pumilio-2-rabbit-monoclonal-antibody-m03347-boster.html2026-03-17T05:12:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03347-pumilio-2-primary-antibodies-wb-testing-1.jpgAnti-Pumilio 2 Rabbit Monoclonal AntibodyWestern blot analysis of Pumilio-2 using anti-Pumilio-2 antibody (M03347). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human REH whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pumilio-2 antigen affinity purified monoclonal antibody (M03347) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Pumilio-2 at approximately 114 kDa. The expected band size for Pumilio-2 is at 114 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dcr2-rabbit-monoclonal-antibody-m05136-2-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05136-2-wb.jpgAnti-DCR2 Rabbit Monoclonal AntibodyWestern blot analysis of DCR2 expression in Human prostate lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05136-2-tnfrsf10d-primary-antibodies-ihc-testing-1.jpgAnti-DCR2 Rabbit Monoclonal AntibodyIHC analysis of DcR2/TNFRSF10D using anti-DcR2/TNFRSF10D antibody (M05136-2). DcR2/TNFRSF10D was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DcR2/TNFRSF10D Antibody (M05136-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05136-2-tnfrsf10d-primary-antibodies-ihc-testing-2.jpgAnti-DCR2 Rabbit Monoclonal AntibodyIHC analysis of DcR2/TNFRSF10D using anti-DcR2/TNFRSF10D antibody (M05136-2). DcR2/TNFRSF10D was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DcR2/TNFRSF10D Antibody (M05136-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05136-2-tnfrsf10d-primary-antibodies-ihc-testing-3.jpgAnti-DCR2 Rabbit Monoclonal AntibodyIHC analysis of DcR2/TNFRSF10D using anti-DcR2/TNFRSF10D antibody (M05136-2). DcR2/TNFRSF10D was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DcR2/TNFRSF10D Antibody (M05136-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ap2-alpha-rabbit-monoclonal-antibody-m01167-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01167-tfap2a-primary-antibodies-wb-testing-1.jpgAnti-AP2 alpha Rabbit Monoclonal AntibodyWestern blot analysis of TFAP2A using anti-TFAP2A antibody (M01167). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFAP2A antigen affinity purified monoclonal antibody (M01167) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFAP2A at approximately 48 kDa. The expected band size for TFAP2A is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tirap-rabbit-monoclonal-antibody-m00950-1-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00950-1-tirap-primary-antibodies-wb-testing-1.jpgAnti-TIRAP Rabbit Monoclonal AntibodyWestern blot analysis of TIRAP using anti-TIRAP antibody (M00950-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIRAP antigen affinity purified monoclonal antibody (M00950-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIRAP at approximately 28 kDa. The expected band size for TIRAP is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sam68-rabbit-monoclonal-antibody-m01717-2-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01717-2-wb7.jpgAnti-SAM68 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01717-2-wb8.jpgAnti-SAM68 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01717-2-wb.jpgAnti-SAM68 Rabbit Monoclonal AntibodyWestern blot analysis of SAM68 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-engrailed-1-rabbit-monoclonal-antibody-m05395-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05395-en1-primary-antibodies-wb-testing-1.jpgAnti-Engrailed 1 Rabbit Monoclonal Antibody Western blot analysis of EN1 using anti-EN1 antibody (M05395). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EN1 antigen affinity purified monoclonal antibody (Catalog # M05395) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EN1 at approximately 55 kDa. The expected band size for EN1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05395-wb.jpgAnti-Engrailed 1 Rabbit Monoclonal AntibodyWestern blot analysis of Engrailed 1 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rad23a-rabbit-monoclonal-antibody-m03243-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03243-wb.jpgAnti-RAD23A Rabbit Monoclonal AntibodyWestern blot analysis of RAD23A expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apoa4-rabbit-monoclonal-antibody-m01973-1-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01973-1-wb.jpgAnti-ApoA4 Rabbit Monoclonal AntibodyWestern blot analysis of ApoA4 expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trprs-rabbit-monoclonal-antibody-m02444-1-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02444-1-wb.jpgAnti-TrpRS Rabbit Monoclonal AntibodyWestern blot analysis of TrpRS expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ung-rabbit-monoclonal-antibody-m01672-2-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01672-2-wb.jpgAnti-UNG Rabbit Monoclonal AntibodyWestern blot analysis of UNG expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mbnl1-rabbit-monoclonal-antibody-m02309-1-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02309-1-wb.jpgAnti-MBNL1 Rabbit Monoclonal AntibodyWestern blot analysis of MBNL1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tf2b-rabbit-monoclonal-antibody-m06893-2-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06893-2-tf2b-primary-antibodies-wb-testing-1.jpgAnti-TF2B Rabbit Monoclonal AntibodyWestern blot analysis of TF2B using anti-TF2B antibody (M06893-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TF2B antigen affinity purified monoclonal antibody (M06893-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TF2B at approximately 31 kDa. The expected band size for TF2B is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-par4-rabbit-monoclonal-antibody-m03637-1-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03637-1-wb7.jpgAnti-PAR4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03637-1-wb.jpgAnti-PAR4 Rabbit Monoclonal AntibodyWestern blot analysis of PAR4 expression in LnCaP cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03637-1-wb8.jpgAnti-PAR4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03637-1-wb9.jpgAnti-PAR4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ada-rabbit-monoclonal-antibody-m00866-1-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-1-wb7.jpgAnti-ADA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-1-wb10.jpgAnti-ADA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-1-wb8.jpgAnti-ADA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-1-wb.jpgAnti-ADA Rabbit Monoclonal AntibodyWestern blot analysis of ADA expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00866-1-wb9.jpgAnti-ADA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-malt1-rabbit-monoclonal-antibody-m01599-2-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01599-2-wb.jpgAnti-MALT1 Rabbit Monoclonal AntibodyWestern blot analysis of MALT1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mage-1-rabbit-monoclonal-antibody-m03570-1-boster.html2026-03-17T05:12:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03570-1-wb.jpgAnti-MAGE 1 Rabbit Monoclonal AntibodyWestern blot analysis of MAGE 1 expression in A375 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acm2-rabbit-monoclonal-antibody-m02733-1-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb7.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb10.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb8.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb11.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb9.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb12.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb13.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb14.jpgAnti-ACM2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02733-1-wb.jpgAnti-ACM2 Rabbit Monoclonal AntibodyWestern blot analysis of ACM2 expression in U87-MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-b7-rabbit-monoclonal-antibody-m00186-2-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00186-2-wb.jpgAnti-HLA B7 Rabbit Monoclonal AntibodyWestern blot analysis of HLA B7 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-card11-rabbit-monoclonal-antibody-m00740-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00740-wb.jpgAnti-CARD11 Rabbit Monoclonal AntibodyWestern blot analysis of CARD11 expression in K562 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00740-wb7.jpgAnti-CARD11 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00740-wb8.jpgAnti-CARD11 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-angiotensinogen-rabbit-monoclonal-antibody-m02103-3-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02103-3-wb.jpgAnti-Angiotensinogen Rabbit Monoclonal AntibodyWestern blot analysis of Angiotensinogen expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acha4-rabbit-monoclonal-antibody-m02230-1-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02230-1-wb.jpgAnti-ACHA4 Rabbit Monoclonal AntibodyWestern blot analysis of ACHA4 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stxbp1-rabbit-monoclonal-antibody-m02351-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02351-wb.jpgAnti-STXBP1 Rabbit Monoclonal AntibodyWestern blot analysis of STXBP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-itch-rabbit-monoclonal-antibody-m00195-2-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00195-2-wb.jpgAnti-ITCH Rabbit Monoclonal AntibodyWestern blot analysis of ITCH/AIP4 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trap1-rabbit-monoclonal-antibody-m02426-4-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02426-4-wb.jpgAnti-TRAP1 Rabbit Monoclonal AntibodyWestern blot analysis of TRAP1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vegfb-rabbit-monoclonal-antibody-m04494-1-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04494-1-vegfb-primary-antibodies-wb-testing-1.jpgAnti-VEGFB Rabbit Monoclonal AntibodyWestern blot analysis of VEGFB using anti-VEGFB antibody (M04494-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: recombinant human VEGFB protein. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified monoclonal antibody (M04494-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VEGFB at approximately 26 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-edd-rabbit-monoclonal-antibody-m03160-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03160-wb.jpgAnti-EDD Rabbit Monoclonal AntibodyWestern blot analysis of EDD expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myf5-rabbit-monoclonal-antibody-m04040-1-boster.html2026-03-17T05:12:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04040-1-wb.jpgAnti-MYF5 Rabbit Monoclonal AntibodyWestern blot analysis of MYF5 expression in Human skeletal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-csnk1a1-rabbit-monoclonal-antibody-m07171-1-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07171-1-wb.jpgAnti-CSNK1A1 Rabbit Monoclonal AntibodyWestern blot analysis of CSNK1A1 expression in (1) MCF7 cell lysate; (2) NIH/3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07171-1-wb7.jpgAnti-CSNK1A1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3k dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07171-1-wb8.jpgAnti-CSNK1A1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3k dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-s1pr3-rabbit-monoclonal-antibody-m03755-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03755-s1pr3-primary-antibodies-wb-testing-1.jpgAnti-S1PR3 Rabbit Monoclonal Antibody Western blot analysis of S1PR3 using anti-S1PR3 antibody (M03755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat NRK whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S1PR3 antigen affinity purified monoclonal antibody (Catalog # M03755) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S1PR3 at approximately 42 kDa. The expected band size for S1PR3 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ggt1-rabbit-monoclonal-antibody-m02340-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02340-wb.jpgAnti-GGT1 Rabbit Monoclonal AntibodyWestern blot analysis of GGT1 expression in Human fetal liver lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thyroid-peroxidase-rabbit-monoclonal-antibody-m00320-2-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00320-2-wb.jpgAnti-Thyroid Peroxidase Rabbit Monoclonal AntibodyWestern blot analysis of Thyroid Peroxidase expression in Human thyroid lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slamf1-rabbit-monoclonal-antibody-m03988-1-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03988-1-wb.jpgAnti-SLAMF1 Rabbit Monoclonal AntibodyWestern blot analysis of SLAMF1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rcc1-rabbit-monoclonal-antibody-m02719-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-wb.jpgAnti-RCC1 Rabbit Monoclonal AntibodyWestern blot analysis of RCC1 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il17a-receptor-rabbit-monoclonal-antibody-m01311-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01311-wb.jpgAnti-IL17A Receptor Rabbit Monoclonal AntibodyWestern blot analysis of IL17A Receptor expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apoh-rabbit-monoclonal-antibody-m01998-1-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01998-1-apoh-primary-antibodies-wb-testing-1.jpgAnti-ApoH Rabbit Monoclonal AntibodyWestern blot analysis of APOH using anti-APOH antibody (M01998-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOH antigen affinity purified monoclonal antibody (M01998-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for APOH at approximately 50 kDa. The expected band size for APOH is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01998-1-apoh-primary-antibodies-ihc-testing-1.jpgAnti-ApoH Rabbit Monoclonal AntibodyIHC analysis of APOH using anti-APOH antibody (M01998-1). APOH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-APOH Antibody (M01998-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01998-1-apoh-primary-antibodies-ihc-testing-2.jpgAnti-ApoH Rabbit Monoclonal AntibodyIHC analysis of APOH using anti-APOH antibody (M01998-1). APOH was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-APOH Antibody (M01998-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01998-1-apoh-primary-antibodies-ihc-testing-3.jpgAnti-ApoH Rabbit Monoclonal AntibodyIHC analysis of APOH using anti-APOH antibody (M01998-1). APOH was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-APOH Antibody (M01998-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nr2e1-rabbit-monoclonal-antibody-m04767-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04767-wb.jpgAnti-NR2E1 Rabbit Monoclonal AntibodyWestern blot analysis of NR2E1 / Tailless expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pannexin-1-rabbit-monoclonal-antibody-m00915-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00915-wb.jpgAnti-Pannexin 1 Rabbit Monoclonal AntibodyWestern blot analysis of Pannexin 1 expression in Caco-2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adam15-rabbit-monoclonal-antibody-m02593-1-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02593-1-wb7.jpgAnti-ADAM15 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02593-1-wb.jpgAnti-ADAM15 Rabbit Monoclonal AntibodyWestern blot analysis of ADAM15 expression in SW480 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kif4a-rabbit-monoclonal-antibody-m04486-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04486-wb.jpgAnti-KIF4A Rabbit Monoclonal AntibodyWestern blot analysis of KIF4A expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ephrin-a1-rabbit-monoclonal-antibody-m04656-boster.html2026-03-17T05:12:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04656-wb.jpgAnti-Ephrin A1 Rabbit Monoclonal AntibodyWestern blot analysis of Ephrin A1 expression in HUVEC cell treated with TNF alpha. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lefty1-lefty2-rabbit-monoclonal-antibody-m06558-2-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06558-2-wb.jpgAnti-LEFTY1 + LEFTY2 Rabbit Monoclonal AntibodyWestern blot analysis of LEFTY1 + LEFTY2 expression in Caco2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chrna5-rabbit-monoclonal-antibody-m02359-1-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02359-1-wb.jpgAnti-CHRNA5 Rabbit Monoclonal AntibodyWestern blot analysis of CHRNA5 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tbx3-rabbit-monoclonal-antibody-m01107-1-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01107-1-wb.jpgAnti-Tbx3 Rabbit Monoclonal AntibodyWestern blot analysis of Tbx3 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glucokinase-rabbit-monoclonal-antibody-m00884-2-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00884-2-wb.jpgAnti-Glucokinase Rabbit Monoclonal AntibodyWestern blot analysis of Glucokinase expression in BxPC-3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wif1-rabbit-monoclonal-antibody-m03247-1-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03247-1-wif1-primary-antibodies-wb-testing-1.jpgAnti-WIF1 Rabbit Monoclonal Antibody Western blot analysis of WIF1 using anti-WIF1 antibody (M03247-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WIF1 antigen affinity purified monoclonal antibody (Catalog # M03247-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WIF1 at approximately 42 kDa. The expected band size for WIF1 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctrl-rabbit-monoclonal-antibody-m08534-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08534-wb.jpgAnti-CTRL Rabbit Monoclonal AntibodyWestern blot analysis of CTRL expression in Mouse pancreas lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thromboxane-synthase-rabbit-monoclonal-antibody-m04697-1-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04697-1-wb.jpgAnti-Thromboxane synthase Rabbit Monoclonal AntibodyWestern blot analysis of Thromboxane synthase expression in Human fetal liver lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fgf19-rabbit-monoclonal-antibody-m01191-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01191-fgf19-primary-antibodies-wb-testing-1.jpgAnti-FGF19 Rabbit Monoclonal Antibody Western blot analysis of FGF19 using anti-FGF19 antibody (M01191). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified monoclonal antibody (Catalog # M01191) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-akr1c1-akr1c2-rabbit-monoclonal-antibody-m03056-1-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03056-1-wb.jpgAnti-AKR1C1 / AKR1C2 Rabbit Monoclonal AntibodyWestern blot analysis of AKR1C1/AKR1C2 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fhit-rabbit-monoclonal-antibody-m01200-3-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01200-3-wb.jpgAnti-FHIT Rabbit Monoclonal AntibodyWestern blot analysis of FHIT expression in Rat kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gemin-3-rabbit-monoclonal-antibody-m05291-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05291-wb.jpgAnti-Gemin 3 Rabbit Monoclonal AntibodyWestern blot analysis of Gemin 3 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glud1-rabbit-monoclonal-antibody-m01866-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01866-glud1-primary-antibodies-wb-testing-1.jpgAnti-GLUD1 Rabbit Monoclonal AntibodyWestern blot analysis of GLUD1 using anti-GLUD1 antibody (M01866). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUD1 antigen affinity purified monoclonal antibody (M01866) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLUD1 at approximately 50 kDa. The expected band size for GLUD1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01866-glud1-primary-antibodies-ihc-testing-2.jpgAnti-GLUD1 Rabbit Monoclonal AntibodyIHC analysis of GLUD1 using anti-GLUD1 antibody (M01866). GLUD1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUD1 Antibody (M01866) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01866-glud1-primary-antibodies-ihc-testing-3.jpgAnti-GLUD1 Rabbit Monoclonal AntibodyIHC analysis of GLUD1 using anti-GLUD1 antibody (M01866). GLUD1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUD1 Antibody (M01866) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01866-glud1-primary-antibodies-ihc-testing-4.jpgAnti-GLUD1 Rabbit Monoclonal AntibodyIHC analysis of GLUD1 using anti-GLUD1 antibody (M01866). GLUD1 was detected in a paraffin-embedded section of mouse midbrain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUD1 Antibody (M01866) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01866-glud1-primary-antibodies-ihc-testing-5.jpgAnti-GLUD1 Rabbit Monoclonal AntibodyIHC analysis of GLUD1 using anti-GLUD1 antibody (M01866). GLUD1 was detected in a paraffin-embedded section of rat midbrain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLUD1 Antibody (M01866) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxf1-rabbit-monoclonal-antibody-m03563-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03563-wb.jpgAnti-FOXF1 Rabbit Monoclonal AntibodyWestern blot analysis of FOXF1 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03563-foxf1-primary-antibodies-ihc-testing-1.jpgAnti-FOXF1 Rabbit Monoclonal AntibodyIHC analysis of FOXF1 using anti-FOXF1 antibody (M03563). FOXF1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FOXF1 Antibody (M03563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03563-foxf1-primary-antibodies-ihc-testing-2.jpgAnti-FOXF1 Rabbit Monoclonal AntibodyIHC analysis of FOXF1 using anti-FOXF1 antibody (M03563). FOXF1 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FOXF1 Antibody (M03563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03563-foxf1-primary-antibodies-ihc-testing-3.jpgAnti-FOXF1 Rabbit Monoclonal AntibodyIHC analysis of FOXF1 using anti-FOXF1 antibody (M03563). FOXF1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FOXF1 Antibody (M03563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psap-rabbit-monoclonal-antibody-m00937-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00937-wb.jpgAnti-PSAP Rabbit Monoclonal AntibodyWestern blot analysis of PSAP expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lox12-rabbit-monoclonal-antibody-m02275-1-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02275-1-wb.jpgAnti-LOX12 Rabbit Monoclonal AntibodyWestern blot analysis of LOX12 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tip49a-rabbit-monoclonal-antibody-m02049-boster.html2026-03-17T05:12:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02049-wb.jpgAnti-TIP49A Rabbit Monoclonal AntibodyWestern blot analysis of TIP49A expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plcg2-rabbit-monoclonal-antibody-m03572-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03572-wb.jpgAnti-PLCG2 Rabbit Monoclonal AntibodyWestern blot analysis of PLCG 2 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hoxb4-rabbit-monoclonal-antibody-m03652-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03652-wb.jpgAnti-HOXB4 Rabbit Monoclonal AntibodyWestern blot analysis of HOXB4 expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03652-wb7.jpgAnti-HOXB4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03652-wb8.jpgAnti-HOXB4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stc1-rabbit-monoclonal-antibody-m03402-1-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03402-1-wb.jpgAnti-STC1 Rabbit Monoclonal AntibodyWestern blot analysis of STC1 expression in Human Prostate lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ebi3-rabbit-monoclonal-antibody-m07190-2-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07190-2-ebi3-primary-antibodies-wb-testing-1.jpgAnti-EBI3 Rabbit Monoclonal Antibody Western blot analysis of EBI3 using anti-EBI3 antibody (M07190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBI3 antigen affinity purified monoclonal antibody (Catalog # M07190-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBI3 at approximately 30 kDa. The expected band size for EBI3 is at 25,30 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plcb3-rabbit-monoclonal-antibody-m04226-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04226-wb.jpgAnti-PLCB3 Rabbit Monoclonal AntibodyWestern blot analysis of PLCB3 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lhx3-rabbit-monoclonal-antibody-m03803-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03803-wb.jpgAnti-LHX3 Rabbit Monoclonal AntibodyWestern blot analysis of LHX3 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hrh3-rabbit-monoclonal-antibody-m06436-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06436-hrh3-primary-antibodies-wb-testing-1.jpgAnti-HRH3 Rabbit Monoclonal Antibody Western blot analysis of HRH3 using anti-HRH3 antibody (M06436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRH3 antigen affinity purified monoclonal antibody (M06436) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRH3 at approximately 45 kDa. The expected band size for HRH3 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-flap-rabbit-monoclonal-antibody-m02341-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02341-wb.jpgAnti-FLAP Rabbit Monoclonal AntibodyWestern blot analysis of FLAP expression in THP1 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grik2-rabbit-monoclonal-antibody-m03374-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03374-wb.jpgAnti-GRIK2 Rabbit Monoclonal AntibodyWestern blot analysis of GRIK2 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcm4-rabbit-monoclonal-antibody-m02301-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02301-wb.jpgAnti-MCM4 Rabbit Monoclonal AntibodyWestern blot analysis of MCM4 expression in Molt-4 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02301-mcm4-primary-antibodies-ihc-testing-2.jpgAnti-MCM4 Rabbit Monoclonal Antibody IHC analysis of MCM4 using anti-MCM4 antibody (M02301). MCM4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MCM4 Antibody (M02301) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02301-mcm4-primary-antibodies-ihc-testing-3.jpgAnti-MCM4 Rabbit Monoclonal Antibody IHC analysis of MCM4 using anti-MCM4 antibody (M02301). MCM4 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MCM4 Antibody (M02301) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ryk-rabbit-monoclonal-antibody-m07225-1-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07225-1-ryk-primary-antibodies-wb-testing-1.jpgAnti-RYK Rabbit Monoclonal Antibody Western blot analysis of RYK using anti-RYK antibody (M07225-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RYK antigen affinity purified monoclonal antibody (Catalog # M07225-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RYK at approximately 72 kDa. The expected band size for RYK is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tead1-rabbit-monoclonal-antibody-m03263-1-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03263-1-tead1-primary-antibodies-wb-testing-1.jpgAnti-TEAD1 Rabbit Monoclonal Antibody Western blot analysis of TEAD1 using anti-TEAD1 antibody (M03263-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD1 antigen affinity purified monoclonal antibody (M03263-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEAD1 at approximately 52 kDa. The expected band size for TEAD1 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rad52-rabbit-monoclonal-antibody-m01580-boster.html2026-03-17T05:12:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01580-wb7.jpgAnti-RAD52 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01580-wb.jpgAnti-RAD52 Rabbit Monoclonal AntibodyWestern blot analysis of RAD52 expression in PC3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fra2-rabbit-monoclonal-antibody-m02615-2-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02615-2-wb.jpgAnti-FRA2 Rabbit Monoclonal AntibodyWestern blot analysis of FRA2 expression in NCCIT cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmeff2-rabbit-monoclonal-antibody-m03846-2-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03846-2-wb.jpgAnti-TMEFF2 Rabbit Monoclonal AntibodyWestern blot analysis of TMEFF2 expression in LnCaP cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-imp2-rabbit-monoclonal-antibody-m02010-1-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02010-1-imp2-primary-antibodies-wb-testing-1.jpgAnti-IMP2 Rabbit Monoclonal Antibody Western blot analysis of IMP2 using anti-IMP2 antibody (M02010-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP2 antigen affinity purified monoclonal antibody (Catalog # M02010-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP2 at approximately 66 kDa. The expected band size for IMP2 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02010-1-imp2-primary-antibodies-ihc-testing-2.jpgAnti-IMP2 Rabbit Monoclonal Antibody IHC analysis of IMP2 using anti-IMP2 antibody (M02010-1). IMP2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IMP2 Antibody (M02010-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02010-1-imp2-primary-antibodies-ihc-testing-3.jpgAnti-IMP2 Rabbit Monoclonal Antibody IHC analysis of IMP2 using anti-IMP2 antibody (M02010-1). IMP2 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IMP2 Antibody (M02010-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-srd5a2-rabbit-monoclonal-antibody-m00704-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00704-srd5a2-primary-antibodies-wb-testing-1.jpgAnti-SRD5A2 Rabbit Monoclonal Antibody Western blot analysis of SRD5A2 using anti-SRD5A2 antibody (M00704). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human NIH/3T3 whole cell lysates, Lane 6: mouse HT22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRD5A2 antigen affinity purified monoclonal antibody (Catalog # M00704) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRD5A2 at approximately 28 kDa. The expected band size for SRD5A2 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-znf217-rabbit-monoclonal-antibody-m03921-2-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03921-2-wb.jpgAnti-ZNF217 Rabbit Monoclonal AntibodyWestern blot analysis of ZNF217 expression in Caco2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-olig1-rabbit-monoclonal-antibody-m06108-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06108-wb.jpgAnti-Olig1 Rabbit Monoclonal AntibodyWestern blot analysis of Olig1 expression in MOLT4 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp7b-rabbit-monoclonal-antibody-m00686-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00686-wb.jpgAnti-ATP7b Rabbit Monoclonal AntibodyWestern blot analysis of ATP7b expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-renin-rabbit-monoclonal-antibody-m03689-1-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03689-1-wb.jpgAnti-Renin Rabbit Monoclonal AntibodyWestern blot analysis of Renin expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klk2-rabbit-monoclonal-antibody-m03879-1-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03879-1-wb.jpgAnti-KLK2 Rabbit Monoclonal AntibodyWestern blot analysis of KLK2 expression in MOLT4 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crkii-rabbit-monoclonal-antibody-m02533-3-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02533-3-crk-primary-antibodies-wb-testing-1.jpgAnti-CRKII Rabbit Monoclonal AntibodyWestern blot analysis of CRKII expression in K562 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02533-3-crkii-primary-antibodies-if-testing-2.jpgAnti-CRKII Rabbit Monoclonal AntibodyIF analysis of CRKII using anti-CRKII antibody (M02533-3) and anti-Beta Tubulin antibody (M01857-3). CRKII was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-CRKII Antibody (M02533-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pias2-rabbit-monoclonal-antibody-m04130-1-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04130-1-wb.jpgAnti-PIAS2 Rabbit Monoclonal AntibodyWestern blot analysis of PIAS2 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clpp-rabbit-monoclonal-antibody-m01117-1-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01117-1-wb.jpgAnti-CLPP Rabbit Monoclonal AntibodyWestern blot analysis of CLPP expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sap97-rabbit-monoclonal-antibody-m01287-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01287-wb.jpgAnti-SAP97 Rabbit Monoclonal AntibodyWestern blot analysis of SAP97 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apoa-rabbit-monoclonal-antibody-m00472-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00472-wb.jpgAnti-ApoA Rabbit Monoclonal AntibodyWestern blot analysis of ApoA expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ube2c-rabbit-monoclonal-antibody-m01491-1-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01491-1-wb.jpgAnti-UBE2C Rabbit Monoclonal AntibodyWestern blot analysis of UBE2C expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-m6pr-rabbit-monoclonal-antibody-m01405-1-boster.html2026-03-17T05:12:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01405-1-wb.jpgAnti-M6PR Rabbit Monoclonal AntibodyWestern blot analysis of M6PR expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sesn1-rabbit-monoclonal-antibody-m05559-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05559-sesn1-primary-antibodies-wb-testing-1.jpgAnti-SESN1 Rabbit Monoclonal Antibody Western blot analysis of SESN1 using anti-SESN1 antibody (M05559). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SESN1 antigen affinity purified monoclonal antibody (Catalog # M05559) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SESN1 at approximately 41 kDa. The expected band size for SESN1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bgat-rabbit-monoclonal-antibody-m00315-1-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00315-1-wb.jpgAnti-BGAT Rabbit Monoclonal AntibodyWestern blot analysis of BGAT expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-csk-rabbit-monoclonal-antibody-m00799-2-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00799-2-wb.jpgAnti-CSK Rabbit Monoclonal AntibodyWestern blot analysis of CSK expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-madcam1-rabbit-monoclonal-antibody-m04227-2-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04227-2-wb.jpgAnti-MADCAM1 Rabbit Monoclonal AntibodyWestern blot analysis of MADCAM1 expression in HUVEC cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnaja1-rabbit-monoclonal-antibody-m04151-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04151-wb.jpgAnti-DNAJA1 Rabbit Monoclonal AntibodyWestern blot analysis of DNAJA1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pim2-rabbit-monoclonal-antibody-m03053-2-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03053-2-pim2-primary-antibodies-wb-testing-1.jpgAnti-PIM2 Rabbit Monoclonal AntibodyWestern blot analysis of PIM2 using anti-PIM2 antibody (M03053-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIM2 antigen affinity purified monoclonal antibody (M03053-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIM2 at approximately 38 kDa. The expected band size for PIM2 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hippocalcin-rabbit-monoclonal-antibody-m03907-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03907-wb.jpgAnti-Hippocalcin Rabbit Monoclonal AntibodyWestern blot analysis of Hippocalcin expression in Neuro2a cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il12-p40-rabbit-monoclonal-antibody-m01152-2-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01152-2-wb.jpgAnti-IL12 p40 Rabbit Monoclonal AntibodyWestern blot analysis of IL12 p40 expression in Human IL12 p40 recombinant protein. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pltp-rabbit-monoclonal-antibody-m02209-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02209-wb.jpgAnti-PLTP Rabbit Monoclonal AntibodyWestern blot analysis of PLTP expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pr3-rabbit-monoclonal-antibody-m03863-2-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03863-2-wb.jpgAnti-PR3 Rabbit Monoclonal AntibodyWestern blot analysis of PR3 expression in HL60 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03863-2-wb7.jpgAnti-PR3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cystatin-a-rabbit-monoclonal-antibody-m03914-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03914-wb.jpgAnti-Cystatin A Rabbit Monoclonal AntibodyWestern blot analysis of Cystatin A expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fra1-rabbit-monoclonal-antibody-m03927-1-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03927-1-fra1-primary-antibodies-wb-testing-1.jpgAnti-FRA1 Rabbit Monoclonal Antibody Western blot analysis of FRA1 using anti-FRA1 antibody (M03927-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human SIHA whole cell lysates, Lane 6: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA1 antigen affinity purified monoclonal antibody (Catalog # M03927-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA1 at approximately 39 kDa. The expected band size for FRA1 is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erap1-rabbit-monoclonal-antibody-m02021-1-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02021-1-wb.jpgAnti-ERAP1 Rabbit Monoclonal AntibodyWestern blot analysis of ERAP1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dctn3-rabbit-monoclonal-antibody-m11728-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11728-wb.jpgAnti-DCTN3 Rabbit Monoclonal AntibodyWestern blot analysis of DCTN3 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dbf4-rabbit-monoclonal-antibody-m01348-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01348-wb.jpgAnti-DBF4 Rabbit Monoclonal AntibodyWestern blot analysis of DBF4 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dp1-rabbit-monoclonal-antibody-m04204-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04204-dp1-primary-antibodies-wb-testing-1.jpgAnti-DP1 Rabbit Monoclonal Antibody Western blot analysis of DP1 using anti-DP1 antibody (M04204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DP1 antigen affinity purified monoclonal antibody (Catalog # M04204) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DP1 at approximately 50 kDa. The expected band size for DP1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04204-dp1-primary-antibodies-ihc-testing-2.jpgAnti-DP1 Rabbit Monoclonal Antibody IHC analysis of DP1 using anti-DP1 antibody (M04204). DP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DP1 Antibody (M04204) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04204-dp1-primary-antibodies-ihc-testing-3.jpgAnti-DP1 Rabbit Monoclonal Antibody IHC analysis of DP1 using anti-DP1 antibody (M04204). DP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-DP1 Antibody (M04204) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irf4-rabbit-monoclonal-antibody-m00401-2-boster.html2026-03-17T05:12:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00401-2-wb.jpgAnti-IRF4 Rabbit Monoclonal AntibodyWestern blot analysis of IRF4 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-senp2-rabbit-monoclonal-antibody-m02329-2-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02329-2-senp2-primary-antibodies-wb-testing-1.jpgAnti-SENP2 Rabbit Monoclonal AntibodyWestern blot analysis of SENP2 using anti-SENP2 antibody (M02329-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP2 antigen affinity purified monoclonal antibody (M02329-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SENP2 at approximately 68 kDa. The expected band size for SENP2 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kifap3-rabbit-monoclonal-antibody-m07239-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07239-wb.jpgAnti-KIFAP3 Rabbit Monoclonal AntibodyWestern blot analysis of KIFAP3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tead1-rabbit-monoclonal-antibody-m03263-2-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03263-2-wb.jpgAnti-TEAD1 Rabbit Monoclonal AntibodyWestern blot analysis of TEAD1 expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03263-2-wb7.jpgAnti-TEAD1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2W dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wrn-rabbit-monoclonal-antibody-m00873-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00873-wrn-primary-antibodies-wb-testing-1.jpgAnti-WRN Rabbit Monoclonal AntibodyWestern blot analysis of WRN using anti-WRN antibody (M00873). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRN antigen affinity purified monoclonal antibody (M00873) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WRN at approximately 200 kDa. The expected band size for WRN is at 162 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmod2-rabbit-monoclonal-antibody-m12585-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12585-wb.jpgAnti-TMOD2 Rabbit Monoclonal AntibodyWestern blot analysis of TMOD2 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdc123-rabbit-monoclonal-antibody-m08251-2-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08251-2-wb.jpgAnti-CDC123 Rabbit Monoclonal AntibodyWestern blot analysis of CDC123 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dldh-rabbit-monoclonal-antibody-m00870-2-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00870-2-wb.jpgAnti-DLDH Rabbit Monoclonal AntibodyWestern blot analysis of DLDH expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00870-2-wb7.jpgAnti-DLDH Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00870-2-wb8.jpgAnti-DLDH Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5W dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-per3-rabbit-monoclonal-antibody-m01835-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01835-per3-primary-antibodies-wb-testing-1.jpgAnti-PER3 Rabbit Monoclonal AntibodyWestern blot analysis of PER3 using anti-PER3 antibody (M01835). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PER3 antigen affinity purified monoclonal antibody (M01835) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PER3 at approximately 132 kDa. The expected band size for PER3 is at 132 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccl21-rabbit-monoclonal-antibody-m00765-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00765-wb.jpgAnti-CCL21 Rabbit Monoclonal AntibodyWestern blot analysis of CCL21 expression in Human CCL21 recombinant protein lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-angptl3-rabbit-monoclonal-antibody-m02929-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb7.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb10.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb8.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb11.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb9.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb12.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb13.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02929-1-wb.jpgAnti-ANGPTL3 Rabbit Monoclonal AntibodyWestern blot analysis of ANGPTL3 expression in A375 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crmp4-rabbit-monoclonal-antibody-m05960-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05960-crmp4-primary-antibodies-wb-testing-1.jpgAnti-CRMP4 Rabbit Monoclonal Antibody Western blot analysis of CRMP4 using anti-CRMP4 antibody (M05960). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRMP4 antigen affinity purified monoclonal antibody (Catalog # M05960) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRMP4 at approximately 62 kDa. The expected band size for CRMP4 is at 62 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-desmoglein-3-rabbit-monoclonal-antibody-m03093-1-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03093-1-wb.jpgAnti-Desmoglein 3 Rabbit Monoclonal AntibodyWestern blot analysis of Desmoglein 3 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdlim1-rabbit-monoclonal-antibody-m04832-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04832-wb.jpgAnti-PDLIM1 Rabbit Monoclonal AntibodyWestern blot analysis of PDLIM1 expression in (1) Saos2 cell lysate; (2) Mouse lung lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-serca1-atpase-rabbit-monoclonal-antibody-m04657-2-boster.html2026-03-17T05:12:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04657-2-wb.jpgAnti-SERCA1 ATPase Rabbit Monoclonal AntibodyWestern blot analysis of SERCA1 ATPase expression in Human fetal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-inhibin-beta-a-rabbit-monoclonal-antibody-m06777-boster.html2026-03-17T05:12:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06777-inhba-primary-antibodies-wb-testing-1.jpgAnti-Inhibin beta A Rabbit Monoclonal Antibody Western blot analysis of Inhibin beta A/INHBA using anti-Inhibin beta A/INHBA antibody (M06777). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Inhibin beta A/INHBA antigen affinity purified monoclonal antibody (M06777) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Inhibin beta A/INHBA at approximately 43 kDa. The expected band size for Inhibin beta A/INHBA is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hira-rabbit-monoclonal-antibody-m03101-boster.html2026-03-17T05:12:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03101-wb.jpgAnti-HIRA Rabbit Monoclonal AntibodyWestern blot analysis of HIRA expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dna-polymerase-gamma-rabbit-monoclonal-antibody-m02796-boster.html2026-03-17T05:12:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02796-dna-polymerase-gamma-primary-antibodies-wb-testing-1.jpgAnti-DNA Polymerase gamma Rabbit Monoclonal AntibodyWestern blot analysis of DNA Polymerase gamma using anti-DNA Polymerase gamma antibody (M02796). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Siha whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase gamma antigen affinity purified monoclonal antibody (M02796) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNA Polymerase gamma at approximately 140 kDa. The expected band size for DNA Polymerase gamma is at 140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02796-polg-primary-antibodies-wb-testing-2_1.jpgAnti-DNA Polymerase gamma Rabbit Monoclonal AntibodyWestern blot analysis of POLG using anti-POLG antibody (M02796). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLG antigen affinity purified monoclonal antibody (M02796) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLG at approximately 140 kDa. The expected band size for POLG is at 140 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ese1-rabbit-monoclonal-antibody-m01027-1-boster.html2026-03-17T05:12:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01027-1-ese1-primary-antibodies-wb-testing-1.jpgAnti-ESE1 Rabbit Monoclonal Antibody Western blot analysis of ESE1 using anti-ESE1 antibody (M01027-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: mouse EL-4 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESE1 antigen affinity purified monoclonal antibody (M01027-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESE1 at approximately 41 kDa. The expected band size for ESE1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01027-1-ese1-primary-antibodies-wb-testing-2.jpgAnti-ESE1 Rabbit Monoclonal AntibodyWestern blot analysis of ESE1 using anti-ESE1 antibody (M01027-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESE1 antigen affinity purified monoclonal antibody (M01027-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESE1 at approximately 41 kDa. The expected band size for ESE1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01027-1-ese1-primary-antibodies-wb-testing-1_1.jpgAnti-ESE1 Rabbit Monoclonal AntibodyWestern blot analysis of P-TSC2 using anti-P-TSC2 antibody (M00229S939). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-TSC2 antigen affinity purified monoclonal antibody (M00229S939) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-TSC2 at approximately 140 kDa. The expected band size for P-TSC2 is at 201 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fabpi-rabbit-monoclonal-antibody-m02378-1-boster.html2026-03-17T05:12:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02378-1-wb.jpgAnti-FABPI Rabbit Monoclonal AntibodyWestern blot analysis of intestinal FABP expression in Human small intestine lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-inhibin-beta-b-rabbit-monoclonal-antibody-m03838-boster.html2026-03-17T05:12:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03838-wb.jpgAnti-Inhibin beta B Rabbit Monoclonal AntibodyWestern blot analysis of Inhibin beta B expression in Mouse brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-arp2-rabbit-monoclonal-antibody-m03898-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03898-arp2-primary-antibodies-wb-testing-1.jpgAnti-Arp2 Rabbit Monoclonal AntibodyWestern blot analysis of Arp2 using anti-Arp2 antibody (M03898). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arp2 antigen affinity purified monoclonal antibody (M03898) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Arp2 at approximately 37 kDa. The expected band size for Arp2 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chx10-rabbit-monoclonal-antibody-m05180-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05180-wb7.jpgAnti-CHX10 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05180-wb10.jpgAnti-CHX10 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05180-ijo-18-04-582-g002.jpgAnti-CHX10 Rabbit Monoclonal AntibodyMorphology and identification of OC-RSCs. A: Passage 0; B: Passage 3; A and B: bar=100 µm; C: Co-expression of CHX10 and BrdU markers; D: Co-expression of PAX6 and BrdU markers, C and D, bar=20 µm. CHX10: Ceh Homeobox 10; PAX6: Paired box 6; BrdU: Bromodeoxyuridine; OC-RSC: Optic-cup-derived retinal stem cells; DAPI: 4′,6-diamidino-2-phenylindole. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11947540/'>40256021</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05180-wb8.jpgAnti-CHX10 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05180-wb.jpgAnti-CHX10 Rabbit Monoclonal AntibodyWestern blot analysis of CHX10 expression in Y79 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05180-wb9.jpgAnti-CHX10 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-naip-rabbit-monoclonal-antibody-m02699-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02699-wb.jpgAnti-NAIP Rabbit Monoclonal AntibodyWestern blot analysis of NAIP expression in Human fetal kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acvr2a-rabbit-monoclonal-antibody-m04770-1-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04770-1-acvr2a-primary-antibodies-wb-testing-1.jpgAnti-ACVR2A Rabbit Monoclonal AntibodyWestern blot analysis of ACVR2A using anti-ACVR2A antibody (M04770-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2A antigen affinity purified monoclonal antibody (M04770-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACVR2A at approximately 85 kDa. The expected band size for ACVR2A is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-5ht7-receptor-rabbit-monoclonal-antibody-m05018-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05018-wb7.jpgAnti-5HT7 Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05018-wb10.jpgAnti-5HT7 Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05018-wb8.jpgAnti-5HT7 Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05018-wb.jpgAnti-5HT7 Receptor Rabbit Monoclonal AntibodyWestern blot analysis of 5HT7 Receptor expression in U-87MG cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05018-wb9.jpgAnti-5HT7 Receptor Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fxr1-rabbit-monoclonal-antibody-m03308-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-fxr1-primary-antibodies-wb-testing-1_1.jpgAnti-FXR1 Rabbit Monoclonal Antibody Western blot analysis of FXR1 using anti-FXR1 antibody (M03308). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXR1 antigen affinity purified monoclonal antibody (Catalog # M03308) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXR1 at approximately 70-80 kDa. The expected band size for FXR1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-fxr1-primary-antibodies-ihc-testing-2.jpgAnti-FXR1 Rabbit Monoclonal Antibody IHC analysis of FXR1 using anti-FXR1 antibody (M03308). FXR1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FXR1 Antibody (M03308) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-fxr1-primary-antibodies-ihc-testing-3.jpgAnti-FXR1 Rabbit Monoclonal Antibody IHC analysis of FXR1 using anti-FXR1 antibody (M03308). FXR1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FXR1 Antibody (M03308) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-wb8.jpgAnti-FXR1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sstr5-rabbit-monoclonal-antibody-m02892-1-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02892-1-wb.jpgAnti-SSTR5 Rabbit Monoclonal AntibodyWestern blot analysis of Somatostatin Receptor 5 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fkbp38-rabbit-monoclonal-antibody-m03722-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03722-wb.jpgAnti-FKBP38 Rabbit Monoclonal AntibodyWestern blot analysis of FKBP38 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-esrrg-rabbit-monoclonal-antibody-m01470-2-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01470-2-esrrg-primary-antibodies-wb-testing-1.jpgAnti-ESRRG Rabbit Monoclonal AntibodyWestern blot analysis of ESRRG using anti-ESRRG antibody (M01470-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRRG antigen affinity purified monoclonal antibody (M01470-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESRRG at approximately 51 kDa. The expected band size for ESRRG is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-celf1-rabbit-monoclonal-antibody-m02163-1-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02163-1-wb.jpgAnti-CELF1 Rabbit Monoclonal AntibodyWestern blot analysis of CUG-BP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcf4-rabbit-monoclonal-antibody-m00674-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00674-wb.jpgAnti-TCF4 Rabbit Monoclonal AntibodyWestern blot analysis of TCF4 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tfpi-rabbit-monoclonal-antibody-m01052-1-boster.html2026-03-17T05:12:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01052-1-wb.jpgAnti-TFPI Rabbit Monoclonal AntibodyWestern blot analysis of TFPI expression in U87-MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oncostatin-m-rabbit-monoclonal-antibody-m00804-1-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00804-1-wb.jpgAnti-Oncostatin M Rabbit Monoclonal AntibodyWestern blot analysis of Oncostatin M expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glb1-rabbit-monoclonal-antibody-m01829-3-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01829-3-glb1-primary-antibodies-ihc-testing-1.jpgAnti-GLB1 Rabbit Monoclonal AntibodyIHC analysis of GLB1 using anti-GLB1 antibody (M01829-3). GLB1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLB1 Antibody (M01829-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01829-3-glb1-primary-antibodies-ihc-testing-2.jpgAnti-GLB1 Rabbit Monoclonal AntibodyIHC analysis of GLB1 using anti-GLB1 antibody (M01829-3). GLB1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLB1 Antibody (M01829-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01829-3-glb1-primary-antibodies-ihc-testing-3.jpgAnti-GLB1 Rabbit Monoclonal AntibodyIHC analysis of GLB1 using anti-GLB1 antibody (M01829-3). GLB1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GLB1 Antibody (M01829-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rhogap-rabbit-monoclonal-antibody-m05701-1-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05701-1-wb.jpgAnti-RhoGAP Rabbit Monoclonal AntibodyWestern blot analysis of RhoGAP expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pi-3-kinase-r4-rabbit-monoclonal-antibody-m06618-1-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06618-1-wb.jpgAnti-PI 3 Kinase R4 Rabbit Monoclonal AntibodyWestern blot analysis of PI 3 Kinase R4 expression in (1) Jurkat cell lysate; (2) Molt-4 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06618-1-pik3r4-primary-antibodies-ihc-testing-1.jpgAnti-PI 3 Kinase R4 Rabbit Monoclonal AntibodyIHC analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (M06618-1). VPS15/PIK3R4 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-VPS15/PIK3R4 Antibody (M06618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06618-1-pik3r4-primary-antibodies-ihc-testing-2.jpgAnti-PI 3 Kinase R4 Rabbit Monoclonal AntibodyIHC analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (M06618-1). VPS15/PIK3R4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-VPS15/PIK3R4 Antibody (M06618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06618-1-pik3r4-primary-antibodies-ihc-testing-3.jpgAnti-PI 3 Kinase R4 Rabbit Monoclonal AntibodyIHC analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (M06618-1). VPS15/PIK3R4 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-VPS15/PIK3R4 Antibody (M06618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dock2-rabbit-monoclonal-antibody-m03836-1-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03836-1-wb.jpgAnti-DOCK2 Rabbit Monoclonal AntibodyWestern blot analysis of DOCK2 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif3e-rabbit-monoclonal-antibody-m00481-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00481-wb.jpgAnti-eIF3e Rabbit Monoclonal AntibodyWestern blot analysis of eIF3e expression in (1) 293T cell lysate; (2) Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stk39-rabbit-monoclonal-antibody-m02516-2-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02516-2-wb7.jpgAnti-STK39 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02516-2-wb10.jpgAnti-STK39 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02516-2-wb8.jpgAnti-STK39 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02516-2-wb.jpgAnti-STK39 Rabbit Monoclonal AntibodyWestern blot analysis of STK39 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02516-2-wb9.jpgAnti-STK39 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wnt5b-rabbit-monoclonal-antibody-m05836-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05836-wb.jpgAnti-Wnt5b Rabbit Monoclonal AntibodyWestern blot analysis of Wnt5b expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc22a3-rabbit-monoclonal-antibody-m04914-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04914-wb.jpgAnti-SLC22A3 Rabbit Monoclonal AntibodyWestern blot analysis of SLC22A3 expression in Human muscle cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04914-slc22a3-primary-antibodies-ihc-testing-1.pngAnti-SLC22A3 Rabbit Monoclonal AntibodyIHC analysis of SLC22A3 using anti-SLC22A3 antibody (M04914). SLC22A3 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:400 rabbit anti-SLC22A3 Antibody (M04914) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cxcl5-rabbit-monoclonal-antibody-m00946-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00946-wb.jpgAnti-CXCL5 Rabbit Monoclonal AntibodyWestern blot analysis of CXCL5 expression in A549 cell lysate treated with TNF alpha and TPA . https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-edg3-rabbit-monoclonal-antibody-m03755-1-boster.html2026-03-17T05:12:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03755-1-s1pr3-primary-antibodies-wb-testing-1.jpgAnti-EDG3 Rabbit Monoclonal Antibody Western blot analysis of EDG3 using anti-EDG3 antibody (M03755-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG3 antigen affinity purified monoclonal antibody (Catalog # M03755-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDG3 at approximately 42 kDa. The expected band size for EDG3 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plap-rabbit-monoclonal-antibody-m05010-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05010-wb.jpgAnti-PLAP Rabbit Monoclonal AntibodyWestern blot analysis of PLAP expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-arf5-rabbit-monoclonal-antibody-m05021-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05021-wb.jpgAnti-ARF5 Rabbit Monoclonal AntibodyWestern blot analysis of ARF5 expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmgn2-rabbit-monoclonal-antibody-m04839-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04839-wb.jpgAnti-HMGN2 Rabbit Monoclonal AntibodyWestern blot analysis of HMGN2 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-abi2-rabbit-monoclonal-antibody-m04302-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04302-wb.jpgAnti-ABI2 Rabbit Monoclonal AntibodyWestern blot analysis of ABI2 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif3b-rabbit-monoclonal-antibody-m04318-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04318-wb.jpgAnti-eIF3B Rabbit Monoclonal AntibodyWestern blot analysis of eIF3B expression in (1) A431 cell lysate; (2) 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eef1g-rabbit-monoclonal-antibody-m10881-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10881-wb.jpgAnti-EEF1G Rabbit Monoclonal AntibodyWestern blot analysis of EEF1G expression in (1) 293T cell lysate; (2) HepG2 cell lysate; (3) NIH/ 3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10881-eef1g-primary-antibodies-ihc-testing-1.jpgAnti-EEF1G Rabbit Monoclonal AntibodyIHC analysis of EEF1G using anti-EEF1G antibody (M10881). EEF1G was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EEF1G Antibody (M10881) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10881-eef1g-primary-antibodies-ihc-testing-2.jpgAnti-EEF1G Rabbit Monoclonal AntibodyIHC analysis of EEF1G using anti-EEF1G antibody (M10881). EEF1G was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EEF1G Antibody (M10881) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10881-eef1g-primary-antibodies-ihc-testing-3.jpgAnti-EEF1G Rabbit Monoclonal AntibodyIHC analysis of EEF1G using anti-EEF1G antibody (M10881). EEF1G was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-EEF1G Antibody (M10881) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-f-rabbit-monoclonal-antibody-m04798-1-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04798-1-wb.jpgAnti-HLA F Rabbit Monoclonal AntibodyWestern blot analysis of HLA F expression in JAR cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neurocan-rabbit-monoclonal-antibody-m06700-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06700-wb.jpgAnti-Neurocan Rabbit Monoclonal AntibodyWestern blot analysis of Neurocan expression in Fetal brain cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ecm1-rabbit-monoclonal-antibody-m02861-1-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02861-1-ecm1-primary-antibodies-wb-testing-1.jpgAnti-ECM1 Rabbit Monoclonal Antibody Western blot analysis of ECM1 using anti-ECM1 antibody (M02861-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM1 antigen affinity purified monoclonal antibody (Catalog # M02861-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECM1 at approximately 75 kDa. The expected band size for ECM1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02861-1-ecm1-primary-antibodies-wb-testing-2.jpgAnti-ECM1 Rabbit Monoclonal Antibody Western blot analysis of ECM1 using anti-ECM1 antibody (M02861-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM1 antigen affinity purified monoclonal antibody (Catalog # M02861-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECM1 at approximately 75 kDa. The expected band size for ECM1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-3.jpgAnti-ECM1 Rabbit Monoclonal Antibody IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1). ECM1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-4.jpgAnti-ECM1 Rabbit Monoclonal Antibody IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1). ECM1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-5.jpgAnti-ECM1 Rabbit Monoclonal Antibody IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1). ECM1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02861-1-ecm1-primary-antibodies-ihc-testing-6.jpgAnti-ECM1 Rabbit Monoclonal Antibody IHC analysis of ECM1 using anti-ECM1 antibody (M02861-1). ECM1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ECM1 Antibody (M02861-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alkbh1-rabbit-monoclonal-antibody-m06945-1-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06945-1-wb7.jpgAnti-ALKBH1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06945-1-wb8.jpgAnti-ALKBH1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06945-1-wb.jpgAnti-ALKBH1 Rabbit Monoclonal AntibodyWestern blot analysis of ALKBH1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd35-rabbit-monoclonal-antibody-m01022-3-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01022-3-wb.jpgAnti-CD35 Rabbit Monoclonal AntibodyWestern blot analysis of CD35 expression in Human tonsil cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-ikb-alpha-s36-rabbit-monoclonal-antibody-p01139-1-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p01139-1-1-nfkbia-primary-antibodies-wb-testing-1.jpgAnti-Phospho-IKB alpha (S36) Rabbit Monoclonal AntibodyWestern blot analysis of IkB Alpha/NFKBIA (Phospho-S36) using anti-IkB Alpha/NFKBIA (Phospho-S36) antibody (P01139-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IkB Alpha/NFKBIA (Phospho-S36) antigen affinity purified monoclonal antibody (P01139-1) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IkB Alpha/NFKBIA (Phospho-S36) at approximately 39 kDa. The expected band size for IkB Alpha/NFKBIA (Phospho-S36) is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-pdha1-s293-rabbit-monoclonal-antibody-p01906-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p01906-pdha1-primary-antibodies-wb-testing-1_1.jpgAnti-Phospho-PDHA1 (S293) Rabbit Monoclonal Antibody Western blot analysis of PDHA1 using anti-PDHA1 antibody (P01906). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDHA1 antigen affinity purified monoclonal antibody (Catalog # P01906) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDHA1 at approximately 43 kDa. The expected band size for PDHA1 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gusb-rabbit-monoclonal-antibody-m02234-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02234-wb.jpgAnti-GUSB Rabbit Monoclonal AntibodyWestern blot analysis of GUSB expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chmp2b-rabbit-monoclonal-antibody-m01935-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01935-chmp2b-primary-antibodies-wb-testing-1.jpgAnti-CHMP2B Rabbit Monoclonal AntibodyWestern blot analysis of CHMP2B using anti-CHMP2B antibody (M01935). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHMP2B antigen affinity purified monoclonal antibody (M01935) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CHMP2B at approximately 28 kDa. The expected band size for CHMP2B is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01935-chmp2b-primary-antibodies-ihc-testing-1.jpgAnti-CHMP2B Rabbit Monoclonal AntibodyIHC analysis of CHMP2B using anti-CHMP2B antibody (M01935). CHMP2B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CHMP2B Antibody (M01935) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01935-chmp2b-primary-antibodies-ihc-testing-2.jpgAnti-CHMP2B Rabbit Monoclonal AntibodyIHC analysis of CHMP2B using anti-CHMP2B antibody (M01935). CHMP2B was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CHMP2B Antibody (M01935) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01935-chmp2b-primary-antibodies-ip-testing-1.jpgAnti-CHMP2B Rabbit Monoclonal AntibodyImmunoprecipitating CHMP2B in A549 whole cell lysate. Western blot analysis of CHMP2B using anti-CHMP2B antibody (M01935). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-CHMP2B antibody in A549 whole cell lysate, Lane 3: anti-CHMP2B antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CHMP2B antigen affinity purified monoclonal antibody (M01935) at a dilution of 1:50 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CHMP2B at approximately 28 kDa. The expected band size for CHMP2B is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stx1a-rabbit-monoclonal-antibody-m01961-3-boster.html2026-03-17T05:12:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01961-3-wb.jpgAnti-STX1A Rabbit Monoclonal AntibodyWestern blot analysis of STX1A expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myh7b-rabbit-monoclonal-antibody-m07574-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07574-wb.jpgAnti-MYH7B Rabbit Monoclonal AntibodyWestern blot analysis of MYH7B expression in Human fetal muscle lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rpl7a-rabbit-monoclonal-antibody-m08246-1-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08246-1-rpl7a-primary-antibodies-wb-testing-1.jpgAnti-RPL7A Rabbit Monoclonal Antibody Western blot analysis of RPL7A using anti-RPL7A antibody (M08246-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL7A antigen affinity purified monoclonal antibody (Catalog # M08246-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL7A at approximately 30 kDa. The expected band size for RPL7A is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08246-1-rpl7a-primary-antibodies-ihc-testing-2.jpgAnti-RPL7A Rabbit Monoclonal Antibody IHC analysis of RPL7A using anti-RPL7A antibody (M08246-1). RPL7A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPL7A Antibody (M08246-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08246-1-rpl7a-primary-antibodies-ihc-testing-3.jpgAnti-RPL7A Rabbit Monoclonal Antibody IHC analysis of RPL7A using anti-RPL7A antibody (M08246-1). RPL7A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPL7A Antibody (M08246-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08246-1-rpl7a-primary-antibodies-ihc-testing-4.jpgAnti-RPL7A Rabbit Monoclonal Antibody IHC analysis of RPL7A using anti-RPL7A antibody (M08246-1). RPL7A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPL7A Antibody (M08246-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-got2-rabbit-monoclonal-antibody-m05998-1-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05998-1-got2-primary-antibodies-wb-testing-1.jpgAnti-GOT2 Rabbit Monoclonal AntibodyWestern blot analysis of GOT2 using anti-GOT2 antibody (M05998-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOT2 antigen affinity purified monoclonal antibody (M05998-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GOT2 at approximately 41 kDa. The expected band size for GOT2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05998-1-got2-primary-antibodies-ihc-testing-1.jpgAnti-GOT2 Rabbit Monoclonal AntibodyIHC analysis of GOT2 using anti-GOT2 antibody (M05998-1). GOT2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GOT2 Antibody (M05998-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05998-1-got2-primary-antibodies-ihc-testing-2.jpgAnti-GOT2 Rabbit Monoclonal AntibodyIHC analysis of GOT2 using anti-GOT2 antibody (M05998-1). GOT2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GOT2 Antibody (M05998-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klc1-rabbit-monoclonal-antibody-m04116-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04116-klc1-primary-antibodies-wb-testing-1.jpgAnti-KLC1 Rabbit Monoclonal AntibodyWestern blot analysis of KLC1 using anti-KLC1 antibody (M04116). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLC1 antigen affinity purified monoclonal antibody (M04116) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KLC1 at approximately 65 kDa. The expected band size for KLC1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04116-ip7.jpgAnti-KLC1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lcat-rabbit-monoclonal-antibody-m00906-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00906-lcat-primary-antibodies-wb-testing-1.jpgAnti-LCAT Rabbit Monoclonal AntibodyWestern blot analysis of LCAT using anti-LCAT antibody (M00906). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCAT antigen affinity purified monoclonal antibody (M00906) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LCAT at approximately 55 kDa. The expected band size for LCAT is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00906-lcat-primary-antibodies-if-testing-1.jpgAnti-LCAT Rabbit Monoclonal AntibodyIF analysis of LCAT using anti-LCAT antibody (M00906). LCAT was detected in an immunocytochemical section of GES-1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-LCAT Antibody (M00906) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-troy-rabbit-monoclonal-antibody-m06157-2-boster.html2026-04-03T05:00:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06157-2-wb7.jpgAnti-TROY Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06157-2-wb8.jpgAnti-TROY Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06157-2-wb.jpgAnti-TROY Rabbit Monoclonal AntibodyWestern blot analysis of TROY expression in LnCaP cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dok1-rabbit-monoclonal-antibody-m03039-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03039-wb.jpgAnti-DOK1 Rabbit Monoclonal AntibodyWestern blot analysis of DOK1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kir2-1-rabbit-monoclonal-antibody-m01850-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01850-wb.jpgAnti-Kir2.1 Rabbit Monoclonal AntibodyWestern blot analysis of Kir2.1 expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gaa-rabbit-monoclonal-antibody-m01548-1-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-1-wb7.jpgAnti-GAA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01548-1-wb.jpgAnti-GAA Rabbit Monoclonal AntibodyWestern blot analysis of GAA expression in Human fetal liver lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aplp2-rabbit-monoclonal-antibody-m01232-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01232-wb.jpgAnti-APLP2 Rabbit Monoclonal AntibodyWestern blot analysis of APLP2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-snap-rabbit-monoclonal-antibody-m05153-2-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05153-2-wb.jpgAnti-Alpha SNAP Rabbit Monoclonal AntibodyWestern blot analysis of Alpha SNAP expression in Molt4 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eph-receptor-b3-rabbit-monoclonal-antibody-m04659-1-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04659-1-ephb3-primary-antibodies-wb-testing-2.jpgAnti-Eph receptor B3 Rabbit Monoclonal AntibodyWestern blot analysis of EPHB3 using anti-EPHB3 antibody (M04659-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB3 antigen affinity purified monoclonal antibody (M04659-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPHB3 at approximately 110 kDa. The expected band size for EPHB3 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04659-1-1-ephb3-primary-antibodies-wb-testing-1.jpgAnti-Eph receptor B3 Rabbit Monoclonal AntibodyWestern blot analysis of EPHB3 using anti-EPHB3 antibody (M04659-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB3 antigen affinity purified monoclonal antibody (M04659-1) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPHB3 at approximately 110 kDa. The expected band size for EPHB3 is at 110 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vegfd-rabbit-monoclonal-antibody-m32333-2-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32333-2-wb.jpgAnti-VEGFD Rabbit Monoclonal AntibodyWestern blot analysis of VEGFD expression in Caco-2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmgcs2-rabbit-monoclonal-antibody-m04371-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04371-wb7.jpgAnti-HMGCS2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04371-wb.jpgAnti-HMGCS2 Rabbit Monoclonal AntibodyWestern blot analysis of HMGCS2 expression in Caco-2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04371-wb8.jpgAnti-HMGCS2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04371-wb9.jpgAnti-HMGCS2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dpyd-rabbit-monoclonal-antibody-m01749-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01749-wb.jpgAnti-DPYD Rabbit Monoclonal AntibodyWestern blot analysis of DPYD expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rgap1-rabbit-monoclonal-antibody-m03018-1-boster.html2026-03-17T05:12:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03018-1-racgap1-primary-antibodies-wb-testing-1.jpgAnti-RGAP1 Rabbit Monoclonal AntibodyWestern blot analysis of RACGAP1 using anti-RACGAP1 antibody (M03018-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RACGAP1 antigen affinity purified monoclonal antibody (M03018-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RACGAP1 at approximately 71 kDa. The expected band size for RACGAP1 is at 71 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nfya-rabbit-monoclonal-antibody-m03524-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03524-nfya-primary-antibodies-wb-testing-1.jpgAnti-NFYA Rabbit Monoclonal AntibodyWestern blot analysis of NFYA using anti-NFYA antibody (M03524). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFYA antigen affinity purified monoclonal antibody (M03524) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFYA at approximately 37 kDa. The expected band size for NFYA is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03524-ip7.jpgAnti-NFYA Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lgi1-rabbit-monoclonal-antibody-m00850-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00850-wb.jpgAnti-Lgi1 Rabbit Monoclonal AntibodyWestern blot analysis of Lgi1 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00850-ip7.jpgAnti-Lgi1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-htsf1-rabbit-monoclonal-antibody-m07890-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-wb.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyWestern blot analysis of HTSF1 expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-htsf1-primary-antibodies-ihc-testing-1.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyIHC analysis of HTSF1 using anti-HTSF1 antibody (M07890). HTSF1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HTSF1 Antibody (M07890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-htsf1-primary-antibodies-ihc-testing-2.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyIHC analysis of HTSF1 using anti-HTSF1 antibody (M07890). HTSF1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HTSF1 Antibody (M07890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-htsf1-primary-antibodies-ihc-testing-3.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyIHC analysis of HTSF1 using anti-HTSF1 antibody (M07890). HTSF1 was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HTSF1 Antibody (M07890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-htsf1-primary-antibodies-ihc-testing-4.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyIHC analysis of HTSF1 using anti-HTSF1 antibody (M07890). HTSF1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HTSF1 Antibody (M07890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-htsf1-primary-antibodies-ihc-testing-5.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyIHC analysis of HTSF1 using anti-HTSF1 antibody (M07890). HTSF1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HTSF1 Antibody (M07890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-htsf1-primary-antibodies-ihc-testing-6.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyIHC analysis of HTSF1 using anti-HTSF1 antibody (M07890). HTSF1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HTSF1 Antibody (M07890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-htsf1-primary-antibodies-ihc-testing-7.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyIHC analysis of HTSF1 using anti-HTSF1 antibody (M07890). HTSF1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HTSF1 Antibody (M07890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07890-ip7.jpgAnti-HTSF1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bat3-rabbit-monoclonal-antibody-m00967-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00967-wb.jpgAnti-BAT3 Rabbit Monoclonal AntibodyWestern blot analysis of BAT3 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pex19-rabbit-monoclonal-antibody-m03186-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03186-wb.jpgAnti-PEX19 Rabbit Monoclonal AntibodyWestern blot analysis of PEX19 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kallikrein-5-rabbit-monoclonal-antibody-m03507-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03507-wb.jpgAnti-Kallikrein 5 Rabbit Monoclonal AntibodyWestern blot analysis of Kallikrein 5 expression in MCF-7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-arg2-rabbit-monoclonal-antibody-m02244-1-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02244-1-wb.jpgAnti-Arg2 Rabbit Monoclonal AntibodyWestern blot analysis of Arg2 expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02244-1-wb9.jpgAnti-Arg2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02244-1-wb7.jpgAnti-Arg2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02244-1-wb10.jpgAnti-Arg2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02244-1-wb8.jpgAnti-Arg2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p4hb-rabbit-monoclonal-antibody-m02335-2-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-2-wb8.jpgAnti-P4HB Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-2-wb.jpgAnti-P4HB Rabbit Monoclonal AntibodyWestern blot analysis of P4HB expression in (1) HepG2 cell lysate; (2) Mouse spleen lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-2-icc1.jpgAnti-P4HB Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-2-icc2.jpgAnti-P4HB Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xpd-rabbit-monoclonal-antibody-m00694-1-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00694-1-ercc2-primary-antibodies-wb-testing-1.jpgAnti-XPD Rabbit Monoclonal AntibodyWestern blot analysis of ERCC2 using anti-ERCC2 antibody (M00694-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC2 antigen affinity purified monoclonal antibody (M00694-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERCC2 at approximately 73 kDa. The expected band size for ERCC2 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00694-1-ercc2-primary-antibodies-ihc-testing-1.jpgAnti-XPD Rabbit Monoclonal AntibodyIHC analysis of ERCC2 using anti-ERCC2 antibody (M00694-1). ERCC2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ERCC2 Antibody (M00694-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00694-1-ercc2-primary-antibodies-ihc-testing-2.jpgAnti-XPD Rabbit Monoclonal AntibodyIHC analysis of ERCC2 using anti-ERCC2 antibody (M00694-1). ERCC2 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ERCC2 Antibody (M00694-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00694-1-ercc2-primary-antibodies-ihc-testing-3.jpgAnti-XPD Rabbit Monoclonal AntibodyIHC analysis of ERCC2 using anti-ERCC2 antibody (M00694-1). ERCC2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ERCC2 Antibody (M00694-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00694-1-ercc2-primary-antibodies-ihc-testing-4.jpgAnti-XPD Rabbit Monoclonal AntibodyIHC analysis of ERCC2 using anti-ERCC2 antibody (M00694-1). ERCC2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ERCC2 Antibody (M00694-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vps28-rabbit-monoclonal-antibody-m06407-1-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06407-1-wb7.jpgAnti-VPS28 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06407-1-icc2.jpgAnti-VPS28 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06407-1-wb8.jpgAnti-VPS28 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06407-1-wb.jpgAnti-VPS28 Rabbit Monoclonal AntibodyWestern blot analysis of VPS28 expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06407-1-icc1.jpgAnti-VPS28 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ranbp9-rabbit-monoclonal-antibody-m03448-2-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03448-2-wb.jpgAnti-RanBP9 Rabbit Monoclonal AntibodyWestern blot analysis of RanBP9 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glycerol-kinase-rabbit-monoclonal-antibody-m02884-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02884-wb.jpgAnti-Glycerol kinase Rabbit Monoclonal AntibodyWestern blot analysis of Glycerol kinase expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fmo3-rabbit-monoclonal-antibody-m01739-2-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01739-2-fmo3-primary-antibodies-wb-testing-1.jpgAnti-FMO3 Rabbit Monoclonal AntibodyWestern blot analysis of FMO3 using anti-FMO3 antibody (M01739-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO3 antigen affinity purified monoclonal antibody (M01739-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FMO3 at approximately 60 kDa. The expected band size for FMO3 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lman1-rabbit-monoclonal-antibody-m03628-3-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03628-3-wb.jpgAnti-LMAN1 Rabbit Monoclonal AntibodyWestern blot analysis of LMAN1 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03628-3-lman1-primary-antibodies-ihc-testing-1.jpgAnti-LMAN1 Rabbit Monoclonal AntibodyIHC analysis of ERGIC-53/LMAN1 using anti-ERGIC-53/LMAN1 antibody (M03628-3). ERGIC-53/LMAN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ERGIC-53/LMAN1 Antibody (M03628-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03628-3-lman1-primary-antibodies-ihc-testing-2.jpgAnti-LMAN1 Rabbit Monoclonal AntibodyIHC analysis of ERGIC-53/LMAN1 using anti-ERGIC-53/LMAN1 antibody (M03628-3). ERGIC-53/LMAN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ERGIC-53/LMAN1 Antibody (M03628-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03628-3-lman1-primary-antibodies-ihc-testing-3.jpgAnti-LMAN1 Rabbit Monoclonal AntibodyIHC analysis of ERGIC-53/LMAN1 using anti-ERGIC-53/LMAN1 antibody (M03628-3). ERGIC-53/LMAN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ERGIC-53/LMAN1 Antibody (M03628-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdlim1-rabbit-monoclonal-antibody-m04832-1-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04832-1-wb.jpgAnti-PDLIM1 Rabbit Monoclonal AntibodyWestern blot analysis of PDLIM1 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-supt5h-rabbit-monoclonal-antibody-m03426-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03426-wb.jpgAnti-SUPT5H Rabbit Monoclonal AntibodyWestern blot analysis of SUPT5H expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lpp-rabbit-monoclonal-antibody-m01240-boster.html2026-03-17T05:12:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01240-wb.jpgAnti-LPP Rabbit Monoclonal AntibodyWestern blot analysis of LPP expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dctn5-rabbit-monoclonal-antibody-m12563-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12563-wb.jpgAnti-DCTN5 Rabbit Monoclonal AntibodyWestern blot analysis of DCTN5 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gemin-2-rabbit-monoclonal-antibody-m04530-1-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04530-1-wb.jpgAnti-Gemin 2 Rabbit Monoclonal AntibodyWestern blot analysis of Gemin 2 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04530-1-ip7.jpgAnti-Gemin 2 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hoxa5-rabbit-monoclonal-antibody-m04018-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04018-wb7.jpgAnti-HOXA5 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04018-wb8.jpgAnti-HOXA5 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04018-wb.jpgAnti-HOXA5 Rabbit Monoclonal AntibodyWestern blot analysis of HOXA5 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hoxa9-rabbit-monoclonal-antibody-m00912-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00912-wb.jpgAnti-HOXA9 Rabbit Monoclonal AntibodyWestern blot analysis of HOXA9 expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00912-wb7.jpgAnti-HOXA9 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smg1-rabbit-monoclonal-antibody-m02589-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02589-wb.jpgAnti-Smg1 Rabbit Monoclonal AntibodyWestern blot analysis of Smg1 expression in Saos2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il32-rabbit-monoclonal-antibody-m03286-1-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03286-1-wb.jpgAnti-IL32 Rabbit Monoclonal AntibodyWestern blot analysis of IL32 expression in Human tonsil lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-anprb-rabbit-monoclonal-antibody-m02251-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02251-wb.jpgAnti-ANPRB Rabbit Monoclonal AntibodyWestern blot analysis of ANPRB expression in Human fetal kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mica-rabbit-monoclonal-antibody-m01366-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01366-wb.jpgAnti-MICA Rabbit Monoclonal AntibodyWestern blot analysis of MICA expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-etv6-rabbit-monoclonal-antibody-m00361-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00361-wb.jpgAnti-ETV6 Rabbit Monoclonal AntibodyWestern blot analysis of ETV6 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pla2g2a-rabbit-monoclonal-antibody-m02259-1-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02259-1-pla2g2a-primary-antibodies-wb-testing-1.jpgAnti-PLA2G2A Rabbit Monoclonal Antibody Western blot analysis of PLA2G2A using anti-PLA2G2A antibody (M02259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G2A antigen affinity purified monoclonal antibody (Catalog # M02259-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G2A at approximately 16 kDa. The expected band size for PLA2G2A is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nidogen-1-rabbit-monoclonal-antibody-m05621-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05621-wb.jpgAnti-Nidogen 1 Rabbit Monoclonal AntibodyWestern blot analysis of Nidogen 1 expression in Human fetal heart lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pros1-rabbit-monoclonal-antibody-m01568-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01568-wb.jpgAnti-PROS1 Rabbit Monoclonal AntibodyWestern blot analysis of PROS1 expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adk-rabbit-monoclonal-antibody-m02193-2-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02193-2-wb.jpgAnti-ADK Rabbit Monoclonal AntibodyWestern blot analysis of ADK expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xrcc3-rabbit-monoclonal-antibody-m01068-1-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01068-1-wb.jpgAnti-XRCC3 Rabbit Monoclonal AntibodyWestern blot analysis of XRCC3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-placental-lactogen-rabbit-monoclonal-antibody-m05177-3-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05177-3-wb.jpgAnti-Placental lactogen Rabbit Monoclonal AntibodyWestern blot analysis of Placental lactogen expression in JAR cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-serping1-rabbit-monoclonal-antibody-m01221-boster.html2026-03-17T05:12:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01221-wb.jpgAnti-SERPING1 Rabbit Monoclonal AntibodyWestern blot analysis of SERPING1 expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wdr4-rabbit-monoclonal-antibody-m10150-1-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10150-1-wdr4-primary-antibodies-wb-testing-1.jpgAnti-WDR4 Rabbit Monoclonal Antibody Western blot analysis of WDR4 using anti-WDR4 antibody (M10150-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR4 antigen affinity purified monoclonal antibody (Catalog # M10150-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR4 at approximately 45 kDa. The expected band size for WDR4 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10150-1-icc1.jpgAnti-WDR4 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc27a4-fatp4-rabbit-monoclonal-antibody-m05299-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05299-wb.jpgAnti-SLC27A4 / FATP4 Rabbit Monoclonal AntibodyWestern blot analysis of SLC27A4 / FATP4 expression in (1) HeLa cell lysate; (2) Mouse kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mat2a-rabbit-monoclonal-antibody-m04557-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04557-wb7.jpgAnti-MAT2A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:8K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04557-wb.jpgAnti-MAT2A Rabbit Monoclonal AntibodyWestern blot analysis of MAT2A expression in (1) Ramos cell lysate;(2) Mouse spleen lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pcbp1-rabbit-monoclonal-antibody-m02636-1-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02636-1-pcbp1-primary-antibodies-wb-testing-1.jpgAnti-PCBP1 Rabbit Monoclonal AntibodyWestern blot analysis of PCBP1 using anti-PCBP1 antibody (M02636-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBP1 antigen affinity purified monoclonal antibody (M02636-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCBP1 at approximately 37 kDa. The expected band size for PCBP1 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccl23-rabbit-monoclonal-antibody-m05808-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05808-wb.jpgAnti-CCL23 Rabbit Monoclonal AntibodyWestern blot analysis of CCL23 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prss2-rabbit-monoclonal-antibody-m05763-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05763-wb.jpgAnti-PRSS2 Rabbit Monoclonal AntibodyWestern blot analysis of PRSS2 expression in Human pancreas lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stf1-rabbit-monoclonal-antibody-m00891-1-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00891-1-wb7.jpgAnti-STF1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00891-1-wb.jpgAnti-STF1 Rabbit Monoclonal AntibodyWestern blot analysis of STF1 expression in Jutkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pde4b-rabbit-monoclonal-antibody-m02228-1-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02228-1-wb.jpgAnti-PDE4B Rabbit Monoclonal AntibodyWestern blot analysis of PDE4B expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp1a2-rabbit-monoclonal-antibody-m02064-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02064-wb.jpgAnti-ATP1A2 Rabbit Monoclonal AntibodyWestern blot analysis of ATP1A2 expression in Human fetal heart lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gcya3-rabbit-monoclonal-antibody-m31778-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31778-wb.jpgAnti-GCYA3 Rabbit Monoclonal AntibodyWestern blot analysis of GCYA3 expression in Human fetal kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nlk-rabbit-monoclonal-antibody-m02091-1-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02091-1-nlk-primary-antibodies-wb-testing-1.jpgAnti-NLK Rabbit Monoclonal Antibody Western blot analysis of NLK using anti-NLK antibody (M02091-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLK antigen affinity purified monoclonal antibody (Catalog # M02091-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLK at approximately 58 kDa. The expected band size for NLK is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jag2-rabbit-monoclonal-antibody-m01428-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01428-wb.jpgAnti-JAG2 Rabbit Monoclonal AntibodyWestern blot analysis of JAG2 expression in HUVEC cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acvr1-rabbit-monoclonal-antibody-m00798-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00798-acvr1-primary-antibodies-wb-testing-1.jpgAnti-ACVR1 Rabbit Monoclonal Antibody Western blot analysis of ACVR1 using anti-ACVR1 antibody (M00798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR1 antigen affinity purified monoclonal antibody (Catalog # M00798) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR1 at approximately 57 kDa. The expected band size for ACVR1 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-monoacylglycerol-lipase-rabbit-monoclonal-antibody-m04317-1-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04317-1-wb.jpgAnti-Monoacylglycerol Lipase Rabbit Monoclonal AntibodyWestern blot analysis of Monoacylglycerol Lipase expression in human heart lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tff2-rabbit-monoclonal-antibody-m07013-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07013-wb.jpgAnti-TFF2 Rabbit Monoclonal AntibodyWestern blot analysis of TFF2 expression in Human stomach lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lmo4-rabbit-monoclonal-antibody-m06555-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06555-lmo4-primary-antibodies-wb-testing-1.jpgAnti-LMO4 Rabbit Monoclonal AntibodyWestern blot analysis of LMO4 using anti-LMO4 antibody (M06555). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO4 antigen affinity purified monoclonal antibody (M06555) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LMO4 at approximately 17 kDa. The expected band size for LMO4 is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-taf1-rabbit-monoclonal-antibody-m02151-boster.html2026-03-17T05:12:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02151-wb.jpgAnti-TAF1 Rabbit Monoclonal AntibodyWestern blot analysis of TAF1 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kgf-rabbit-monoclonal-antibody-m01931-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01931-wb.jpgAnti-KGF Rabbit Monoclonal AntibodyWestern blot analysis of KGF expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fsh-beta-rabbit-monoclonal-antibody-m01885-3-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01885-3-wb.jpgAnti-FSH beta Rabbit Monoclonal AntibodyWestern blot analysis of FSH beta expression in Human pituitary lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gjb1-rabbit-monoclonal-antibody-m01050-1-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01050-1-wb.jpgAnti-GJB1 Rabbit Monoclonal AntibodyWestern blot analysis of GJB1 expression in MCF-7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atrip-rabbit-monoclonal-antibody-m03862-1-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03862-1-atrip-primary-antibodies-wb-testing-1.jpgAnti-ATRIP Rabbit Monoclonal Antibody Western blot analysis of ATRIP using anti-ATRIP antibody (M03862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATRIP antigen affinity purified monoclonal antibody (M03862-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATRIP at approximately 90 kDa. The expected band size for ATRIP is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03862-1-atrip-primary-antibodies-ihc-testing-2.jpgAnti-ATRIP Rabbit Monoclonal Antibody IHC analysis of ATRIP using anti-ATRIP antibody (M03862-1) . ATRIP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATRIP Antibody (M03862-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-col11a1-rabbit-monoclonal-antibody-m02909-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02909-wb.jpgAnti-COL11A1 Rabbit Monoclonal AntibodyWestern blot analysis of COL11A1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nme2-rabbit-monoclonal-antibody-m01762-1-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01762-1-nme2-primary-antibodies-wb-testing-1.jpgAnti-NME2 Rabbit Monoclonal AntibodyWestern blot analysis of NME2 using anti-NME2 antibody (M01762-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NME2 antigen affinity purified monoclonal antibody (M01762-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NME2 at approximately 17 kDa. The expected band size for NME2 is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cntp2-rabbit-monoclonal-antibody-m02819-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02819-wb7.jpgAnti-CNTP2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02819-wb10.jpgAnti-CNTP2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02819-wb8.jpgAnti-CNTP2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02819-wb.jpgAnti-CNTP2 Rabbit Monoclonal AntibodyWestern blot analysis of CNTP2 expression in Mouse brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02819-wb9.jpgAnti-CNTP2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp4b-rabbit-monoclonal-antibody-m08719-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08719-wb.jpgAnti-ATP4B Rabbit Monoclonal AntibodyWestern blot analysis of ATP4B expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08719-wb7.jpgAnti-ATP4B Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08719-wb8.jpgAnti-ATP4B Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pitx3-rabbit-monoclonal-antibody-m03453-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03453-pitx3-primary-antibodies-wb-testing-1.jpgAnti-PITX3 Rabbit Monoclonal AntibodyWestern blot analysis of PITX3 using anti-PITX3 antibody (M03453). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITX3 antigen affinity purified monoclonal antibody (M03453) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PITX3 at approximately 36 kDa. The expected band size for PITX3 is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mglur3-rabbit-monoclonal-antibody-m04827-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04827-wb.jpgAnti-mGluR3 Rabbit Monoclonal AntibodyWestern blot analysis of mGluR3 expression in Human cerebellum lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dynamin-3-rabbit-monoclonal-antibody-m06890-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06890-wb.jpgAnti-Dynamin 3 Rabbit Monoclonal AntibodyWestern blot analysis of Dynamin 3 expression in Human fetal brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ercc8-rabbit-monoclonal-antibody-m04153-1-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04153-1-wb.jpgAnti-ERCC8 Rabbit Monoclonal AntibodyWestern blot analysis of ERCC8 expression in Molt4 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-complement-factor-b-rabbit-monoclonal-antibody-m01260-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01260-wb.jpgAnti-Complement factor B Rabbit Monoclonal AntibodyWestern blot analysis of Complement factor B expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fetuin-a-rabbit-monoclonal-antibody-m01251-1-boster.html2026-03-17T05:12:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01251-1-wb.jpgAnti-Fetuin A Rabbit Monoclonal AntibodyWestern blot analysis of Fetuin A expression in Human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lhx2-rabbit-monoclonal-antibody-m05940-3-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05940-3-wb.jpgAnti-LHX2 Rabbit Monoclonal AntibodyWestern blot analysis of LHX2 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05940-3-wb9.jpgAnti-LHX2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05940-3-wb7.jpgAnti-LHX2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05940-3-wb8.jpgAnti-LHX2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd1c-rabbit-monoclonal-antibody-m01188-3-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01188-3-wb.jpgAnti-CD1c Rabbit Monoclonal AntibodyWestern blot analysis of CD1c expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbr1-rabbit-monoclonal-antibody-m02825-2-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-2-wb.jpgAnti-CBR1 Rabbit Monoclonal AntibodyWestern blot analysis of CBR1 expression in MCF-7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsd3b1-rabbit-monoclonal-antibody-m02856-1-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02856-1-wb.jpgAnti-HSD3B1 Rabbit Monoclonal AntibodyWestern blot analysis of HSD3B1 expression in Human placenta lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pde2a-rabbit-monoclonal-antibody-m03472-2-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03472-2-pde2a-primary-antibodies-wb-testing-1.jpgAnti-PDE2A Rabbit Monoclonal AntibodyWestern blot analysis of PDE2A using anti-PDE2A antibody (M03472-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE2A antigen affinity purified monoclonal antibody (M03472-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDE2A at approximately 106 kDa. The expected band size for PDE2A is at 106 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prok1-rabbit-monoclonal-antibody-m05751-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05751-wb.jpgAnti-PROK1 Rabbit Monoclonal AntibodyWestern blot analysis of PROK1 expression in Raw264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pag-rabbit-monoclonal-antibody-m03711-1-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03711-1-wb.jpgAnti-PAG Rabbit Monoclonal AntibodyWestern blot analysis of PAG expression in Raji cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03711-1-pag1-primary-antibodies-ihc-testing-1.jpgAnti-PAG Rabbit Monoclonal AntibodyIHC analysis of PAG1 using anti-PAG1 antibody (M03711-1). PAG1 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAG1 Antibody (M03711-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03711-1-pag1-primary-antibodies-ihc-testing-2.jpgAnti-PAG Rabbit Monoclonal AntibodyIHC analysis of PAG1 using anti-PAG1 antibody (M03711-1). PAG1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAG1 Antibody (M03711-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03711-1-pag1-primary-antibodies-ihc-testing-3.jpgAnti-PAG Rabbit Monoclonal AntibodyIHC analysis of PAG1 using anti-PAG1 antibody (M03711-1). PAG1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PAG1 Antibody (M03711-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ap1g1-rabbit-monoclonal-antibody-m12998-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12998-wb.jpgAnti-AP1G1 Rabbit Monoclonal AntibodyWestern blot analysis of AP1G1 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nup62-rabbit-monoclonal-antibody-m03950-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03950-nup62-primary-antibodies-wb-testing-1_1.jpgAnti-NUP62 Rabbit Monoclonal Antibody Western blot analysis of NUP62 using anti-NUP62 antibody (M03950). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP62 antigen affinity purified monoclonal antibody (Catalog # M03950) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP62 at approximately 70 kDa. The expected band size for NUP62 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03950-nup62-primary-antibodies-if-testing-2.jpgAnti-NUP62 Rabbit Monoclonal Antibody IF analysis of NUP62 using anti-NUP62 antibody (M03950). NUP62 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-NUP62 Antibody (M03950) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dmap1-rabbit-monoclonal-antibody-m03688-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03688-wb.jpgAnti-DMAP1 Rabbit Monoclonal AntibodyWestern blot analysis of DMAP1 expression in Hela cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03688-wb7.jpgAnti-DMAP1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03688-wb8.jpgAnti-DMAP1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctnna3-rabbit-monoclonal-antibody-m05039-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05039-wb.jpgAnti-CTNNA3 Rabbit Monoclonal AntibodyWestern blot analysis of CTNNA3 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-traf2-rabbit-monoclonal-antibody-m00400-2-boster.html2026-03-17T05:12:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00400-2-wb.jpgAnti-TRAF2 Rabbit Monoclonal AntibodyWestern blot analysis of TRAF2 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cc2d1a-rabbit-monoclonal-antibody-m04775-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04775-wb7.jpgAnti-CC2D1A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04775-wb.jpgAnti-CC2D1A Rabbit Monoclonal AntibodyWestern blot analysis of CC2D1A expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-12-rabbit-monoclonal-antibody-m05227-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05227-krt12-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 12 Rabbit Monoclonal Antibody Western blot analysis of KRT12 using anti-KRT12 antibody (M05227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT12 antigen affinity purified monoclonal antibody (Catalog # M05227) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT12 at approximately 54 kDa. The expected band size for KRT12 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mbd3-rabbit-monoclonal-antibody-m02571-3-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02571-3-mbd3-primary-antibodies-wb-testing-1_1.jpgAnti-MBD3 Rabbit Monoclonal Antibody Western blot analysis of MBD3 using anti-MBD3 antibody (M02571-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD3 antigen affinity purified monoclonal antibody (Catalog # M02571-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBD3 at approximately 33, 34, 36 kDa. The expected band size for MBD3 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02571-3-mbd3-primary-antibodies-if-testing-2.jpgAnti-MBD3 Rabbit Monoclonal Antibody IF analysis of MBD3 using anti-MBD3 antibody (M02571-3) and anti-Beta Tubulin antibody (M01857-3). MBD3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-MBD3 Antibody (M02571-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p66-alpha-rabbit-monoclonal-antibody-m09194-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-p66-alpha-primary-antibodies-wb-testing-1.jpgAnti-p66 alpha Rabbit Monoclonal Antibody Western blot analysis of p66 alpha using anti-p66 alpha antibody (M09194). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p66 alpha antigen affinity purified monoclonal antibody (Catalog # M09194) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p66 alpha at approximately 68 kDa. The expected band size for p66 alpha is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-10.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-8.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-9.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-6.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-7.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-5.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-4.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-3.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-2.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09194-gatad2a-primary-antibodies-ihc-testing-1.jpgAnti-p66 alpha Rabbit Monoclonal AntibodyIHC analysis of GATAD2A using anti-GATAD2A antibody (M09194). GATAD2A was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GATAD2A Antibody (M09194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nat10-rabbit-monoclonal-antibody-m06226-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06226-nat10-primary-antibodies-wb-testing-1_1.jpgAnti-NAT10 Rabbit Monoclonal Antibody Western blot analysis of NAT10 using anti-NAT10 antibody (M06226). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAT10 antigen affinity purified monoclonal antibody (Catalog # M06226) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAT10 at approximately 116 kDa. The expected band size for NAT10 is at 116 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rchy1-rabbit-monoclonal-antibody-m04533-1-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04533-1-wb.jpgAnti-RCHY1 Rabbit Monoclonal AntibodyWestern blot analysis of RCHY1 expression in 293 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04533-1-wb7.jpgAnti-RCHY1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1k dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ehmt2-rabbit-monoclonal-antibody-m01055-1-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01055-1-wb.jpgAnti-EHMT2 Rabbit Monoclonal AntibodyWestern blot analysis of EHMT2 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mlkl-rabbit-monoclonal-antibody-m00535-1-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00535-1-mlkl-primary-antibodies-wb-testing-1.jpgAnti-MLKL Rabbit Monoclonal Antibody Western blot analysis of MLKL using anti-MLKL antibody (M00535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLKL antigen affinity purified monoclonal antibody (Catalog # M00535-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLKL at approximately 54 kDa. The expected band size for MLKL is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cirbp-rabbit-monoclonal-antibody-m04103-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-cirbp-primary-antibodies-wb-testing-1.jpgAnti-CIRBP Rabbit Monoclonal AntibodyWestern blot analysis of CIRBP using anti-CIRBP antibody (M04103). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIRBP antigen affinity purified monoclonal antibody (M04103) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CIRBP at approximately 19 kDa. The expected band size for CIRBP is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-cirbp-primary-antibodies-ihc-testing-1.jpgAnti-CIRBP Rabbit Monoclonal AntibodyIHC analysis of CIRBP using anti-CIRBP antibody (M04103). CIRBP was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-CIRBP Antibody (M04103) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-cirbp-primary-antibodies-ihc-testing-2.jpgAnti-CIRBP Rabbit Monoclonal AntibodyIHC analysis of CIRBP using anti-CIRBP antibody (M04103). CIRBP was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-CIRBP Antibody (M04103) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-cirbp-primary-antibodies-ihc-testing-3.jpgAnti-CIRBP Rabbit Monoclonal AntibodyIHC analysis of CIRBP using anti-CIRBP antibody (M04103). CIRBP was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-CIRBP Antibody (M04103) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-cirbp-primary-antibodies-ihc-testing-4.jpgAnti-CIRBP Rabbit Monoclonal AntibodyIHC analysis of CIRBP using anti-CIRBP antibody (M04103). CIRBP was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-CIRBP Antibody (M04103) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-cirbp-primary-antibodies-ihc-testing-5.jpgAnti-CIRBP Rabbit Monoclonal AntibodyIHC analysis of CIRBP using anti-CIRBP antibody (M04103). CIRBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-CIRBP Antibody (M04103) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caveolin-3-rabbit-monoclonal-antibody-m00990-2-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00990-2-wb7.jpgAnti-Caveolin-3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00990-2-wb.jpgAnti-Caveolin-3 Rabbit Monoclonal AntibodyWestern blot analysis of Caveolin-3 expression in human skeletal muscle cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bclg-rabbit-monoclonal-antibody-m09347-1-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09347-1-bcl2l14-primary-antibodies-wb-testing-1.jpgAnti-BCLG Rabbit Monoclonal Antibody Western blot analysis of BCL2L14 using anti-BCL2L14 antibody (M09347-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2L14 antigen affinity purified monoclonal antibody (M09347-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCL2L14 at approximately 45 kDa. The expected band size for BCL2L14 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09347-1-bcl2l14-primary-antibodies-ihc-testing-2.jpgAnti-BCLG Rabbit Monoclonal Antibody IHC analysis of BCL2L14 using anti-BCL2L14 antibody (M09347-1). BCL2L14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BCL2L14 Antibody (M09347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09347-1-bcl2l14-primary-antibodies-ihc-testing-3.jpgAnti-BCLG Rabbit Monoclonal Antibody IHC analysis of BCL2L14 using anti-BCL2L14 antibody (M09347-1). BCL2L14 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BCL2L14 Antibody (M09347-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09347-1-bcl2l14-primary-antibodies-if-testing-1.jpgAnti-BCLG Rabbit Monoclonal AntibodyIF analysis of BCL2L14 using anti-BCL2L14 antibody (M09347-1) . BCL2L14 was detected in an immunocytochemical section of mouse B16 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with rabbit anti-BCL2L14 Antibody (M09347-1) at a dilution of 10 μg/mL overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd147-rabbit-monoclonal-antibody-m00248-3-boster.html2026-03-17T05:12:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-3-wb.jpgAnti-CD147 Rabbit Monoclonal AntibodyWestern blot analysis of CD147 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ahcy-rabbit-monoclonal-antibody-m04236-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04236-wb.jpgAnti-AHCY Rabbit Monoclonal AntibodyWestern blot analysis of AHCY expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-mono-methyl-k9-rabbit-monoclonal-antibody-m12477-11-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-11-wb.jpgAnti-Histone H3 (mono methyl K9) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H3 (mono methyl K9) expression in Hela cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-11-wb9.jpgAnti-Histone H3 (mono methyl K9) Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-11-wb7.jpgAnti-Histone H3 (mono methyl K9) Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-11-wb8.jpgAnti-Histone H3 (mono methyl K9) Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-acetyl-k18-rabbit-monoclonal-antibody-m12477-12-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-12-wb.jpgAnti-Histone H3 (acetyl K18) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H3 (acetyl K18) expression in HeLa cell treated with TSA cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-acetyl-k23-rabbit-monoclonal-antibody-m12477-13-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-13-wb7.jpgAnti-Histone H3 (acetyl K23) Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-13-wb.jpgAnti-Histone H3 (acetyl K23) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H3 (acetyl K23) expression in HeLa cell treated with TSA cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-13-wb8.jpgAnti-Histone H3 (acetyl K23) Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-13-wb9.jpgAnti-Histone H3 (acetyl K23) Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-mutated-k27-met-rabbit-monoclonal-antibody-m12477-14-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-14-wb.jpgAnti-Histone H3 (mutated K27 Met) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H3 (mutated K27 Met) expression in recombinant protein lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-acetyl-k27-rabbit-monoclonal-antibody-m12477-15-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-15-histone_h3-primary-antibodies-ihc-testing-1.pngAnti-Histone H3 (acetyl K27) Rabbit Monoclonal AntibodyIHC analysis of Histone H3 (acetyl K27) using anti-Histone H3 (acetyl K27) antibody (M12477-15). Histone H3 (acetyl K27) was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Histone H3 (acetyl K27) (M12477-15) overnight at 4°C. Polymer anti-rabbit IgG-HRP IHC detection kit was used as secondary antibody and incubated for 45 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-15-histone_h3-primary-antibodies-wb-testing-1.jpgAnti-Histone H3 (acetyl K27) Rabbit Monoclonal Antibody Western blot analysis of Histone H3 using anti-Histone H3 antibody (M12477-15). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified monoclonal antibody (Catalog # M12477-15) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 15 kDa. The expected band size for Histone H3 is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-facl4-rabbit-monoclonal-antibody-m04372-1-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04372-1-facl4-primary-antibodies-wb-testing-1.jpgAnti-FACL4 Rabbit Monoclonal Antibody Western blot analysis of FACL4 using anti-FACL4 antibody (M04372-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4 antigen affinity purified monoclonal antibody (Catalog # M04372-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4 at approximately 79 kDa. The expected band size for FACL4 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04372-1-icc1.jpgAnti-FACL4 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04372-1-icc2.jpgAnti-FACL4 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cox-iv-rabbit-monoclonal-antibody-m05442-3-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-3-cox4i1-primary-antibodies-wb-testing-1.jpgAnti-COX IV Rabbit Monoclonal Antibody Western blot analysis of COX4I1 using anti-COX4I1 antibody (M05442-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX4I1 antigen affinity purified monoclonal antibody (M05442-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX4I1 at approximately 17 kDa. The expected band size for COX4I1 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-3-cox4i1-primary-antibodies-ihc-testing-2.jpgAnti-COX IV Rabbit Monoclonal Antibody IHC analysis of COX4I1 using anti-COX4I1 antibody (M05442-3) . COX4I1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX4I1 Antibody (M05442-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-3-cox4i1-primary-antibodies-ihc-testing-3.jpgAnti-COX IV Rabbit Monoclonal Antibody IHC analysis of COX4I1 using anti-COX4I1 antibody (M05442-3) . COX4I1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX4I1 Antibody (M05442-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-3-cox4i1-primary-antibodies-ihc-testing-4.jpgAnti-COX IV Rabbit Monoclonal Antibody IHC analysis of COX4I1 using anti-COX4I1 antibody (M05442-3) . COX4I1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX4I1 Antibody (M05442-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05442-3-icc1.jpgAnti-COX IV Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:500 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caspase-1-p10-p12-rabbit-monoclonal-antibody-m00048-2-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-casp1-primary-antibodies-wb-testing-1.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal Antibody Western blot analysis of Caspase-1 using anti-Caspase-1 antibody (M00048-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: mouse EL-4 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-1 antigen affinity purified monoclonal antibody (Catalog # M00048-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-1 at approximately 45 kDa. The expected band size for Caspase-1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-wb10.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-ctm2-15-e70337-g008.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal AntibodyAblation of ISG15 decreased pyroptosis of TECs under HG stimulation. (A) Western blot analysis and densitometric quantification of pyroptosis‐related proteins (NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N) expression in kidney tissues from WT and KO mice treated with vehicle or STZ ( n = 6). (B) Level of IL‐18 in the serum from WT and KO mice treated with vehicle or STZ ( n = 6). (C) Western blot analysis of pyroptosis‐related proteins (NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N) expression in TECs ( n = 3). (D) Flow cytometry analysis and quantitative data depicting the TECs Annexin V/PI double‐positive cells rate ( n = 3). (E) CCK‐8‐kit activity assay quantified cell viability ( n = 3). (F–I) Level of LDH (F), IL‐18 (G), ROS (H and I) in TECs ( n = 3). TECs were transfected with sinc (50 nM) or si‐ Isg15 (50 nM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-ctm2-15-e70337-g010.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal AntibodyThe cGAS–STING pathway was activated in the DKD mice. (A) Western blot analysis and densitometric quantification of cGAS, STING, TBK1, p‐TBK1, p65, p‐p65 expression in DKD mice ( n = 6). (B) Western blot analysis and densitometric quantification of NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N expression in TECs ( n = 3). (C) Western blot analysis ISG15/ISGylation expression in TECs ( n = 3). (D) Flow cytometry analysis and quantitative data depicting the Annexin V/PI double‐positive cells rate ( n = 3). (E–G) Levels of LDH (E), IL‐18 (F), ROS (G) in TECs ( n = 3). (H) Relative mRNA level of pro‐inflammatory factors ( Il6 , Tnfa , Mcp1 , Il18 ) in TECs ( n = 3). (I) Western blot analysis and densitometric quantification of KIM1, α‐SMA and Vimentin expression in TECs ( n = 3). TECs were transfected with vehicle or C‐176 (10 µM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-ctm2-15-e70337-g004.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal AntibodyISG15–STING loop‐maintained HG‐induced injury in TECs. (A) Western blot analysis and densitometric quantification of NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD and GSDMD‐N expression in TECs ( n = 3). (B) Flow cytometry analysis and quantitative data depicting the Annexin V/PI double‐positive cells rate ( n = 3). (C–E) Levels of ROS (C), IL‐18 (D), LDH (E) in TECs ( n = 3). TECs were transfected with oe‐STING (4 µg) or si‐ Isg15 (50 nM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-ctm2-15-e70337-g006.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal AntibodyISG15 contributed to TECs injury in an ISGylation‐dependent manner. (A) Western blot analysis and densitometric quantification of cGAS, STING, TBK1, p‐TBK1, p65, p‐p65 expression in TECs ( n = 3). (B) Western blot analysis and densitometric quantification of NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N expression in TECs ( n = 3). (C) Flow cytometry analysis and quantitative data depicting the Annexin V/PI double‐positive cells rate ( n = 3). (D) CCK‐8 activity assay quantified cell viability ( n = 3). (E–G) Levels of ROS (E), LDH (F), IL‐18 (G) in TECs ( n = 3). (H) Western blot analysis and densitometric quantification of KIM1, α‐SMA and Vimentin expression in TECs ( n = 3). (I) Relative mRNA level of pro‐inflammatory factors ( Il6 , Tnfa , Mcp1 and Il18 ) in TECs ( n = 3). TECs were transfected with empty vector, ISG15AA or ISG15 (4 µg). Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-wb7.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-2-wb11.jpgAnti-Caspase-1 + p10 + p12 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:500 dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cofilin-rabbit-monoclonal-antibody-m01525-boster.html2026-03-24T05:36:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01525-cfl1-primary-antibodies-wb-testing-1.jpgAnti-Cofilin Rabbit Monoclonal AntibodyWestern blot analysis of Cofilin/CFL1 using anti-Cofilin/CFL1 antibody (M01525). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin/CFL1 antigen affinity purified monoclonal antibody (M01525) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cofilin/CFL1 at approximately 19 kDa. The expected band size for Cofilin/CFL1 is at 19 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ranbp3-rabbit-monoclonal-antibody-m06252-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06252-wb.jpgAnti-RANBP3 Rabbit Monoclonal AntibodyWestern blot analysis of RANBP3 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pim2-rabbit-monoclonal-antibody-m03053-3-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03053-3-pim2-primary-antibodies-wb-testing-1.jpgAnti-PIM2 Rabbit Monoclonal Antibody Western blot analysis of PIM2 using anti-PIM2 antibody (M03053-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIM2 antigen affinity purified monoclonal antibody (M03053-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIM2 at approximately 34-44 kDa. The expected band size for PIM2 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-htf9c-rabbit-monoclonal-antibody-m13996-1-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13996-1-wb.jpgAnti-HTF9C Rabbit Monoclonal AntibodyWestern blot analysis of HTF9C expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fads1-rabbit-monoclonal-antibody-m02535-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02535-fads1-primary-antibodies-wb-testing-1.jpgAnti-FADS1 Rabbit Monoclonal AntibodyWestern blot analysis of FADS1 using anti-FADS1 antibody (M02535). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS1 antigen affinity purified monoclonal antibody (M02535) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FADS1 at approximately 52 kDa. The expected band size for FADS1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02535-fads1-primary-antibodies-ihc-testing-1.jpgAnti-FADS1 Rabbit Monoclonal AntibodyIHC analysis of FADS1 using anti-FADS1 antibody (M02535). FADS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FADS1 Antibody (M02535) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02535-fads1-primary-antibodies-ihc-testing-2.jpgAnti-FADS1 Rabbit Monoclonal AntibodyIHC analysis of FADS1 using anti-FADS1 antibody (M02535). FADS1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FADS1 Antibody (M02535) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02535-fads1-primary-antibodies-ihc-testing-3.jpgAnti-FADS1 Rabbit Monoclonal AntibodyIHC analysis of FADS1 using anti-FADS1 antibody (M02535). FADS1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FADS1 Antibody (M02535) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02535-fads1-primary-antibodies-ihc-testing-4.jpgAnti-FADS1 Rabbit Monoclonal AntibodyIHC analysis of FADS1 using anti-FADS1 antibody (M02535). FADS1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FADS1 Antibody (M02535) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-qk1-rabbit-monoclonal-antibody-m01874-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-2_1.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-wb-testing-1.jpgAnti-QK1 Rabbit Monoclonal Antibody Western blot analysis of QK1 using anti-QK1 antibody (M01874). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QK1 antigen affinity purified polyclonal antibody (Catalog # M01874) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for QK1 at approximately 38 kDa. The expected band size for QK1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-3_1.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-4_1.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-5_1.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-6.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-7.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-8.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-9.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-10.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qki-primary-antibodies-ihc-testing-11_1.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-QK1 Antibody (M01874) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-ihc-testing-12.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-QK1 Antibody (M01874) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-ihc-testing-13.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of mouse brain cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-QK1 Antibody (M01874) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-ihc-testing-14.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-QK1 Antibody (M01874) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-ihc-testing-15.jpgAnti-QK1 Rabbit Monoclonal Antibody IHC analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-QK1 Antibody (M01874) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-16.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-17.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human melanoma cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-18.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-19.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-20.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-21.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-22.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01874-qk1-primary-antibodies-if-testing-23.jpgAnti-QK1 Rabbit Monoclonal Antibody IF analysis of QK1 using anti-QK1 antibody (M01874). QK1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-QK1 Antibody (M01874) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/c/a/casestudy_m01874.jpgAnti-QK1 Rabbit Monoclonal Antibody https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-exosc7-rabbit-monoclonal-antibody-m11141-1-boster.html2026-03-17T05:12:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11141-1-wb.jpgAnti-EXOSC7 Rabbit Monoclonal AntibodyWestern blot analysis of EXOSC7 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-themis-rabbit-monoclonal-antibody-m07854-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07854-wb.jpgAnti-Themis Rabbit Monoclonal AntibodyWestern blot analysis of Themis expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ube4b-rabbit-monoclonal-antibody-m04338-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04338-wb.jpgAnti-UBE4B Rabbit Monoclonal AntibodyWestern blot analysis of UBE4B expression in (1) 293T cell lysate; (2) NIH/3T3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cars-rabbit-monoclonal-antibody-m00259-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00259-wb.jpgAnti-CARS Rabbit Monoclonal AntibodyWestern blot analysis of CARS expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-drb4-rabbit-monoclonal-antibody-m02570-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02570-wb.jpgAnti-HLA-DRB4 Rabbit Monoclonal AntibodyWestern blot analysis of HLA-DRB4 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-desmocollin-3-rabbit-monoclonal-antibody-m05095-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05095-dsc3-primary-antibodies-wb-testing-1.jpgAnti-Desmocollin-3 Rabbit Monoclonal AntibodyWestern blot analysis of DSC3 using anti-DSC3 antibody (M05095). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSC3 antigen affinity purified monoclonal antibody (M05095) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DSC3 at approximately 100 kDa. The expected band size for DSC3 is at 100 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mrpl28-rabbit-monoclonal-antibody-m12699-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12699-wb.jpgAnti-MRPL28 Rabbit Monoclonal AntibodyWestern blot analysis of MRPL28expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-serpinb3-rabbit-monoclonal-antibody-m04067-2-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04067-2-wb.jpgAnti-SerpinB3 Rabbit Monoclonal AntibodyWestern blot analysis of SerpinB3 expression in A431 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04067-2-serpinb3-primary-antibodies-ihc-testing-1.pngAnti-SerpinB3 Rabbit Monoclonal Antibody IHC analysis of SerpinB3 using anti-SerpinB3 antibody (M04067-2). SerpinB3 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:400 rabbit anti-SerpinB3 Antibody (M04067-2) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uchl3-rabbit-monoclonal-antibody-m05004-1-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05004-1-uchl3-primary-antibodies-wb-testing-1_1.jpgAnti-UCHL3 Rabbit Monoclonal Antibody Western blot analysis of UCHL3 using anti-UCHL3 antibody (M05004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCHL3 antigen affinity purified monoclonal antibody (Catalog # M05004-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCHL3 at approximately 26 kDa. The expected band size for UCHL3 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05004-1-uchl3-primary-antibodies-if-testing-1.jpgAnti-UCHL3 Rabbit Monoclonal AntibodyIF analysis of UCHL3 using anti-UCHL3 antibody (M05004-1) and anti-Tubulin Alpha antibody (M03989-3). UCHL3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-UCHL3 Antibody (M05004-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG Secondary Antibody (red)(BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctbp2-rabbit-monoclonal-antibody-m02567-2-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-2-wb.jpgAnti-CTBP2 Rabbit Monoclonal AntibodyWestern blot analysis of CTBP2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-exosc7-rabbit-monoclonal-antibody-m11141-2-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11141-2-wb.jpgAnti-EXOSC7 Rabbit Monoclonal AntibodyWestern blot analysis of EXOSC7 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alkbh1-rabbit-monoclonal-antibody-m06945-2-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06945-2-wb7.jpgAnti-ALKBH1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06945-2-wb8.jpgAnti-ALKBH1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06945-2-wb.jpgAnti-ALKBH1 Rabbit Monoclonal AntibodyWestern blot analysis of ALKBH1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ube2b-rabbit-monoclonal-antibody-m05190-1-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05190-1-wb.jpgAnti-UBE2B Rabbit Monoclonal AntibodyWestern blot analysis of UBE2B expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gtf2f2-rabbit-monoclonal-antibody-m10896-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10896-wb.jpgAnti-GTF2F2 Rabbit Monoclonal AntibodyWestern blot analysis of GTF2F2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ap2s1-rabbit-monoclonal-antibody-m07176-1-boster.html2026-03-17T05:12:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07176-1-wb.jpgAnti-AP2S1 Rabbit Monoclonal AntibodyWestern blot analysis of AP2S1 expression in (1) 293 cell lysate; (2) Mouse brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gtf2i-rabbit-monoclonal-antibody-m00903-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00903-wb.jpgAnti-GTF2I Rabbit Monoclonal AntibodyWestern blot analysis of GTF2I expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pgd-rabbit-monoclonal-antibody-m01623-1-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01623-1-wb.jpgAnti-PGD Rabbit Monoclonal AntibodyWestern blot analysis of PGD expression in (1) 293 cell lysate; (2) Mouse kidney lysate; (3) Rat kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-collagen-iii-rabbit-monoclonal-antibody-m00788-1-boster.html2026-03-25T05:24:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-1-wb7.jpgAnti-Collagen III Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-1-wb.jpgAnti-Collagen III Rabbit Monoclonal AntibodyWestern blot analysis of Collagen III expression in (1) A431 cell lysate; (2) MCF7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-1-wb8.jpgAnti-Collagen III Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-1-wb9.jpgAnti-Collagen III Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-1-collagen3-primary-antibodies-wb-review-1.pngAnti-Collagen III Rabbit Monoclonal Antibody Western blot analysis of Collagen III using anti-Collagen III antibody (M00788-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: cardiac tissue lysates from 3-month-old mice, Lane 2: cardiac tissue lysates from 6-month-old mice, Lane 3: cardiac tissue lysates from 12-month-old mice, Lane 4: cardiac tissue lysates from 18-month-old mice. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III antigen affinity purified polyclonal antibody (Catalog # M00788-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Collagen III at approximately 139 kDa. The expected band size for Collagen III is at 138 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tdp43-rabbit-monoclonal-antibody-m01001-2-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-2-wb.jpgAnti-TDP43 Rabbit Monoclonal AntibodyWestern blot analysis of TDP43 expression in (1) HeLa cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-2-ihc.jpgAnti-TDP43 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human glioma, using TDP43 Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-2-if.jpgAnti-TDP43 Rabbit Monoclonal AntibodyImmunofluorescent analysis of Hela cells, using TDP43 Antibody .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-2-kd1.jpgAnti-TDP43 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2000 dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccl27-rabbit-monoclonal-antibody-m05293-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05293-wb.jpgAnti-CCL27 Rabbit Monoclonal AntibodyWestern blot analysis of CCL27 expression in Human CCL27 recombinant protein cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gbp1-rabbit-monoclonal-antibody-m03067-1-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03067-1-wb.jpgAnti-GBP1 Rabbit Monoclonal AntibodyWestern blot analysis of GBP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fibronectin-rabbit-monoclonal-antibody-m00564-3-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-fibronectin-primary-antibodies-wb-testing-1.jpgAnti-Fibronectin Rabbit Monoclonal Antibody Western blot analysis of Fibronectin using anti-Fibronectin antibody (M00564-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibronectin antigen affinity purified monoclonal antibody (Catalog # M00564-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibronectin at approximately 272 kDa. The expected band size for Fibronectin is at 272 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-fibronectin-primary-antibodies-ihc-testing-2.jpgAnti-Fibronectin Rabbit Monoclonal Antibody IHC analysis of Fibronectin using anti-Fibronectin antibody (M00564-3). Fibronectin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Fibronectin Antibody (M00564-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-fbioe-08-00446-g002.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyFluorescence images of tenocytes after 5 days of culture on the surface of a culture plate (control group) and acellular amniotic membrane (amnion group). Tenocytes presented a clear cytoskeleton, good biocompatibility with acellular amniotic membrane, and even distribution on the surface of the materials, showing better growth activity than the control group (A,B) . Cell viability was measured by CCK-8, and the proliferation curve of tenocytes in the control and amniotic membrane groups was drawn (C) . Western blot assay for collagen I, fibronectin, TGF-β1, and bFGF expression in the tenocytes of the control and amnion groups for 1 week (D,E) . * p < 0.05; ** p < 0.01; and *** p < 0.001. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00446/full'>32478059</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-fbioe-08-00446-g004.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyRepresentative immunofluorescence images of fibronectin. Fluorescent micrographs of tenocytes after 2 and 5 days of culture on the surface of a culture plate and acellular amniotic membrane. Tenocyte nucleus shape observed under a fluorescence microscope (A,D,G,J) . Fibronectin presented positive after the fluorescent FITC mark was observed under a fluorescence microscope (B,E,H,K) . Tenocyte nucleus and fibronectin merging (C,F,I,L) . The corresponding semi quantitative analysis of fibronectin fluorescence intensity in panels (M,N) (scale bar = 50 um, n = 5, * P < 0.05). Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00446/full'>32478059</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-13567_2018_610_fig9_html.pngAnti-Fibronectin Rabbit Monoclonal AntibodyrFBA and fibronectin interaction analysis by Far-WB and Surface plasmon resonance (SPR) analysis. A Far-WB analysis of rFBA with fibronectin. The first lane: PVDF membrane with transferred rFBA protein incubated with anti- rFBA antibody as a positive control; the second lane: PVDF membrane with transferred rFBA protein incubated with fibronectin and anti-fibronectin antibody; the third lane: PVDF membrane with transferred BSA (negative control) incubated with fibronectin and anti-fibronectin antibody. Protein bands were visualized using ECL substrate. B Sensorgrams depict the binding of immobilised fibronectin to rFBA. Increasing concentrations of rFBA (5, 10, 25, 50 and 100 μg/mL) were injected at a flow rate of 30 μL/min for 180 s over immobilised fibronectin. The arrow indicates the end of the injection period, at which point dissociation of rFBA from fibronectin can be observed. RU resonance units. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13567-018-0610-2'>30454073</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-ajtr0009-5708-f2.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyIdentification of human periodontal ligament cells (PDLCs). Immunofluorescence staining showed that cultured PDLCs were positive for fibronectin and vimentin, and negative for pan cytokeratin, indicating their mesenchymal origin. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5752921/&orig_host=www.ncbi.nlm.nih.gov'>29312523</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-ijcep0007-4827-f1.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyIdentification of trabecular meshwork (TM) cells. (A-G) Representative images of TM cells from one of four cell lines established from the eyes of four donors. Cells from all four lines exhibited similar morphological and immunocytochemical features. The cells were observed by light microscopy (×40) (A) and transmission electron microscopy (×8,000) (B). Immunocytochemical staining of laminin (C) (×400), fibronectin (D) (×400), vimentin (E) (×400), neuron-specific enolase (F) (×400), and Factor VIII-associated antigen (G) (×400). Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4152043/&orig_host=www.ncbi.nlm.nih.gov'>25197353</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-3-fibronectin-primary-antibodies-ihc-testing-3.jpgAnti-Fibronectin Rabbit Monoclonal Antibody IHC analysis of Fibronectin using anti-Fibronectin antibody (M00564-3). Fibronectin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Fibronectin Antibody (M00564-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ncs1-rabbit-monoclonal-antibody-m05330-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05330-wb.jpgAnti-NCS1 Rabbit Monoclonal AntibodyWestern blot analysis of NCS1 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-olig3-rabbit-monoclonal-antibody-m11400-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11400-wb.jpgAnti-Olig3 Rabbit Monoclonal AntibodyWestern blot analysis of Olig3 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nudt5-rabbit-monoclonal-antibody-m07530-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07530-wb7.jpgAnti-NUDT5 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07530-wb10.jpgAnti-NUDT5 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07530-wb8.jpgAnti-NUDT5 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07530-wb.jpgAnti-NUDT5 Rabbit Monoclonal AntibodyWestern blot analysis of NUDT5 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07530-wb9.jpgAnti-NUDT5 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp24-rabbit-monoclonal-antibody-m08241-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08241-wb.jpgAnti-USP24 Rabbit Monoclonal AntibodyWestern blot analysis of USP24 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kappa-light-chain-rabbit-monoclonal-antibody-m15795-boster.html2026-03-17T05:12:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m15795-wb.jpgAnti-Kappa light chain Rabbit Monoclonal AntibodyWestern blot analysis of Kappa light chain expression in Human plasma cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-med4-rabbit-monoclonal-antibody-m06467-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06467-wb.jpgAnti-MED4 Rabbit Monoclonal AntibodyWestern blot analysis of MED4 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-igf2bp2-rabbit-monoclonal-antibody-m02010-2-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02010-2-wb.jpgAnti-IGF2BP2 Rabbit Monoclonal AntibodyWestern blot analysis of IGF2BP2 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crmp3-rabbit-monoclonal-antibody-m12176-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12176-wb.jpgAnti-CRMP3 Rabbit Monoclonal AntibodyWestern blot analysis of CRMP3 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-upp1-rabbit-monoclonal-antibody-m10128-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10128-upp1-primary-antibodies-wb-testing-1.jpgAnti-UPP1 Rabbit Monoclonal Antibody Western blot analysis of UPP1 using anti-UPP1 antibody (M10128). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPP1 antigen affinity purified monoclonal antibody (M10128) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPP1 at approximately 28 kDa. The expected band size for UPP1 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-snrpa1-rabbit-monoclonal-antibody-m10655-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10655-wb.jpgAnti-SNRPA1 Rabbit Monoclonal AntibodyWestern blot analysis of SNRPA1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acaa2-rabbit-monoclonal-antibody-m08341-1-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08341-1-acaa2-primary-antibodies-wb-testing-1_1.jpgAnti-ACAA2 Rabbit Monoclonal Antibody Western blot analysis of ACAA2 using anti-ACAA2 antibody (M08341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAA2 antigen affinity purified monoclonal antibody (Catalog # M08341-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAA2 at approximately 42 kDa. The expected band size for ACAA2 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08341-1-acaa2-primary-antibodies-ihc-testing-2.jpgAnti-ACAA2 Rabbit Monoclonal Antibody IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1). ACAA2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ACAA2 Antibody (M08341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08341-1-acaa2-primary-antibodies-ihc-testing-3.jpgAnti-ACAA2 Rabbit Monoclonal Antibody IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1). ACAA2 was detected in a paraffin-embedded section of human colung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ACAA2 Antibody (M08341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08341-1-acaa2-primary-antibodies-ihc-testing-4.jpgAnti-ACAA2 Rabbit Monoclonal Antibody IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1). ACAA2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ACAA2 Antibody (M08341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08341-1-acaa2-primary-antibodies-ihc-testing-5.jpgAnti-ACAA2 Rabbit Monoclonal Antibody IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1). ACAA2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ACAA2 Antibody (M08341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08341-1-acaa2-primary-antibodies-if-testing-6.jpgAnti-ACAA2 Rabbit Monoclonal Antibody IF analysis of ACAA2 using anti-ACAA2 antibody (M08341-1). ACAA2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-ACAA2 Antibody (M08341-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ube4a-rabbit-monoclonal-antibody-m11871-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11871-wb.jpgAnti-UBE4A Rabbit Monoclonal AntibodyWestern blot analysis of UBE4A expression in 293 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11871-wb7.jpgAnti-UBE4A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11871-wb8.jpgAnti-UBE4A Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:6K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-suox-rabbit-monoclonal-antibody-m05838-1-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05838-1-suox-primary-antibodies-wb-testing-1.jpgAnti-SUOX Rabbit Monoclonal AntibodyWestern blot analysis of SUOX using anti-SUOX antibody (M05838-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUOX antigen affinity purified monoclonal antibody (M05838-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUOX at approximately 60 kDa. The expected band size for SUOX is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05838-1-suox-primary-antibodies-ihc-testing-1.jpgAnti-SUOX Rabbit Monoclonal AntibodyIHC analysis of SUOX using anti-SUOX antibody (M05838-1). SUOX was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SUOX Antibody (M05838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05838-1-suox-primary-antibodies-ihc-testing-2.jpgAnti-SUOX Rabbit Monoclonal AntibodyIHC analysis of SUOX using anti-SUOX antibody (M05838-1). SUOX was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SUOX Antibody (M05838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-brms1-rabbit-monoclonal-antibody-m03587-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03587-wb.jpgAnti-BRMS1 Rabbit Monoclonal AntibodyWestern blot analysis of BRMS1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdgf-rabbit-monoclonal-antibody-m01057-1-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01057-1-wb.jpgAnti-HDGF Rabbit Monoclonal AntibodyWestern blot analysis of HDGF expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctr1-slc31a1-rabbit-monoclonal-antibody-m03447-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03447-slc31a1-primary-antibodies-wb-testing-1_1.jpgAnti-CTR1/SLC31A1 Rabbit Monoclonal Antibody Western blot analysis of CTR1/SLC31A1 using anti-CTR1/SLC31A1 antibody (M03447). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTR1/SLC31A1 antigen affinity purified monoclonal antibody (Catalog # M03447) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTR1/SLC31A1 at approximately 29 kDa. The expected band size for CTR1/SLC31A1 is at 21 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erlin-2-rabbit-monoclonal-antibody-m07042-boster.html2026-03-17T05:12:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-erlin2-primary-antibodies-wb-testing-1.jpgAnti-Erlin-2 Rabbit Monoclonal Antibody Western blot analysis of ERLIN2 using anti-ERLIN2 antibody (M07042). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERLIN2 antigen affinity purified monoclonal antibody (M07042) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERLIN2 at approximately 43 kDa. The expected band size for ERLIN2 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-wb7.jpgAnti-Erlin-2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sbds-rabbit-monoclonal-antibody-m02396-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02396-wb.jpgAnti-SBDS Rabbit Monoclonal AntibodyWestern blot analysis of SBDS expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chd3-rabbit-monoclonal-antibody-m03200-1-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03200-1-wb.jpgAnti-CHD3 Rabbit Monoclonal AntibodyWestern blot analysis of CHD3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd58-rabbit-monoclonal-antibody-m02800-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02800-wb.jpgAnti-CD58 Rabbit Monoclonal AntibodyWestern blot analysis of CD58 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mvd-rabbit-monoclonal-antibody-m01631-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01631-wb.jpgAnti-MVD Rabbit Monoclonal AntibodyWestern blot analysis of MVD expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp39-rabbit-monoclonal-antibody-m06922-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06922-wb.jpgAnti-USP39 Rabbit Monoclonal AntibodyWestern blot analysis of USP39 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mrrf-rabbit-monoclonal-antibody-m09708-1-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09708-1-wb.jpgAnti-MRRF Rabbit Monoclonal AntibodyWestern blot analysis of MRRF expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-human-igg1-rabbit-monoclonal-antibody-m04575-6-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04575-6-wb.jpgAnti-Human IgG1 Rabbit Monoclonal AntibodyWestern blot analysis of human IgG1 expression in Human tonsil cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camkii-rabbit-monoclonal-antibody-m03241-2-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03241-2-camk2abdg-primary-antibodies-wb-testing-1.jpgAnti-CaMKII Rabbit Monoclonal AntibodyWestern blot analysis of CAMK2A/B/D/G using anti-CAMK2A/B/D/G antibody (M03241-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMK2A/B/D/G antigen affinity purified monoclonal antibody (M03241-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAMK2A/B/D/G at approximately 60 kDa. The expected band size for CAMK2A/B/D/G is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03241-2-camk2a-b-d-g-primary-antibodies-ihc-testing-1.jpgAnti-CaMKII Rabbit Monoclonal AntibodyIHC analysis of CAMK2A/B/D/G using anti-CAMK2A/B/D/G antibody (M03241-2). CAMK2A/B/D/G was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CAMK2A/B/D/G Antibody (M03241-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03241-2-camk2a-b-d-g-primary-antibodies-ihc-testing-2.jpgAnti-CaMKII Rabbit Monoclonal AntibodyIHC analysis of CAMK2A/B/D/G using anti-CAMK2A/B/D/G antibody (M03241-2). CAMK2A/B/D/G was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CAMK2A/B/D/G Antibody (M03241-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h2b-formyl-k108-rabbit-monoclonal-antibody-m07286-1-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07286-1-wb.jpgAnti-Histone H2B (formyl K108) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H2B (formyl K108) expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h2b-formyl-k120-rabbit-monoclonal-antibody-m07286-2-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07286-2-wb.jpgAnti-Histone H2B (formyl K120) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H2B (formyl K120) expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h2b-mono-methyl-k116-rabbit-monoclonal-antibody-m07286-3-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07286-3-wb.jpgAnti-Histone H2B (mono methyl K116) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H2B (mono methyl K116) expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-formyl-k122-rabbit-monoclonal-antibody-m12477-16-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-16-wb.jpgAnti-Histone H3 (formyl K122) Rabbit Monoclonal AntibodyWestern blot analysis of Histone H3 (formyl K122) expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd133-rabbit-monoclonal-antibody-m01767-5-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01767-5-cd133-primary-antibodies-ihc-testing-2.jpgAnti-CD133 Rabbit Monoclonal Antibody IHC analysis of CD133 using anti-CD133 antibody (M01767-5). CD133 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD133 Antibody (M01767-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01767-5-cd133-primary-antibodies-wb-testing-1.jpgAnti-CD133 Rabbit Monoclonal Antibody Western blot analysis of CD133 using anti-CD133 antibody (M01767-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD133 antigen affinity purified monoclonal antibody (Catalog # M01767-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD133 at approximately 115 kDa. The expected band size for CD133 is at 97 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phd3-rabbit-monoclonal-antibody-m02182-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02182-wb.jpgAnti-PHD3 Rabbit Monoclonal AntibodyWestern blot analysis of PHD3 expression in (1) A549 cell lysate; (2) NIH/3T3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rgma-rabbit-monoclonal-antibody-m04984-2-boster.html2026-03-17T05:12:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04984-2-wb.jpgAnti-RGMA Rabbit Monoclonal AntibodyWestern blot analysis of RGMA expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-osteoprotegerin-rabbit-monoclonal-antibody-m00863-1-boster.html2026-03-17T05:12:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd79b-rabbit-monoclonal-antibody-m01399-3-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01399-3-cd79b-primary-antibodies-wb-testing-1.jpgAnti-CD79b Rabbit Monoclonal AntibodyWestern blot analysis of CD79b using anti-CD79b antibody (M01399-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79b antigen affinity purified monoclonal antibody (M01399-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD79b at approximately 35 kDa. The expected band size for CD79b is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glu2b-rabbit-monoclonal-antibody-m04992-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04992-wb.jpgAnti-GLU2B Rabbit Monoclonal AntibodyWestern blot analysis of GLU2B expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mgst1-rabbit-monoclonal-antibody-m04703-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04703-mgst1-primary-antibodies-wb-testing-1_1.jpgAnti-MGST1 Rabbit Monoclonal AntibodyWestern blot analysis of MGST1 using anti-MGST1 antibody (M04703). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGST1 antigen affinity purified monoclonal antibody (M04703) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MGST1 at approximately 18 kDa. The expected band size for MGST1 is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctnnbip1-rabbit-monoclonal-antibody-m03185-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03185-ctnnbip1-primary-antibodies-wb-testing-1.jpgAnti-CTNNBIP1 Rabbit Monoclonal AntibodyWestern blot analysis of CTNNBIP1 using anti-CTNNBIP1 antibody (M03185). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNBIP1 antigen affinity purified monoclonal antibody (M03185) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CTNNBIP1 at approximately 12 kDa. The expected band size for CTNNBIP1 is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-coasy-rabbit-monoclonal-antibody-m09138-1-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09138-1-wb.jpgAnti-COASY Rabbit Monoclonal AntibodyWestern blot analysis of COASY expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ncf4-rabbit-monoclonal-antibody-m04208-1-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04208-1-wb7.jpgAnti-NCF4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04208-1-wb8.jpgAnti-NCF4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04208-1-wb.jpgAnti-NCF4 Rabbit Monoclonal AntibodyWestern blot analysis of NCF4 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hfe-rabbit-monoclonal-antibody-m00506-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00506-wb.jpgAnti-HFE Rabbit Monoclonal AntibodyWestern blot analysis of HFE expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-dmb-rabbit-monoclonal-antibody-m03526-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03526-wb.jpgAnti-HLA DMB Rabbit Monoclonal AntibodyWestern blot analysis of HLA DMB expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tub1-rabbit-monoclonal-antibody-m02917-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02917-wb.jpgAnti-TUB1 Rabbit Monoclonal AntibodyWestern blot analysis of TUB1 expression in Neuro-2a cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ltb4r-rabbit-monoclonal-antibody-m04622-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04622-wb.jpgAnti-LTB4R Rabbit Monoclonal AntibodyWestern blot analysis of LTB4R expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-arpc2-rabbit-monoclonal-antibody-m06165-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06165-arpc2-primary-antibodies-wb-testing-1.jpgAnti-ARPC2 Rabbit Monoclonal AntibodyWestern blot analysis of ARPC2 using anti-ARPC2 antibody (M06165). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARPC2 antigen affinity purified monoclonal antibody (M06165) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARPC2 at approximately 34 kDa. The expected band size for ARPC2 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fukutin-rabbit-monoclonal-antibody-m03344-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03344-wb.jpgAnti-Fukutin Rabbit Monoclonal AntibodyWestern blot analysis of Fukutin expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acat2-rabbit-monoclonal-antibody-m03245-2-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03245-2-acat2-primary-antibodies-wb-testing-1.jpgAnti-ACAT2 Rabbit Monoclonal Antibody Western blot analysis of ACAT2 using anti-ACAT2 antibody (M03245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT2 antigen affinity purified monoclonal antibody (Catalog # M03245-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAT2 at approximately 41 kDa. The expected band size for ACAT2 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hgd-rabbit-monoclonal-antibody-m01909-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01909-wb.jpgAnti-HGD Rabbit Monoclonal AntibodyWestern blot analysis of HGD expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prostein-rabbit-monoclonal-antibody-m11232-boster.html2026-03-17T05:12:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11232-wb.jpgAnti-Prostein Rabbit Monoclonal AntibodyWestern blot analysis of Prostein expression in LnCaP cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-znf259-rabbit-monoclonal-antibody-m06916-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06916-znf259-primary-antibodies-wb-testing-1.jpgAnti-ZNF259 Rabbit Monoclonal AntibodyWestern blot analysis of ZNF259 using anti-ZNF259 antibody (M06916). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF259 antigen affinity purified monoclonal antibody (M06916) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ZNF259 at approximately 51 kDa. The expected band size for ZNF259 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gsto1-rabbit-monoclonal-antibody-m02461-1-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02461-1-wb.jpgAnti-GSTO1 Rabbit Monoclonal AntibodyWestern blot analysis of GSTO1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdcd7-rabbit-monoclonal-antibody-m14830-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14830-wb.jpgAnti-PDCD7 Rabbit Monoclonal AntibodyWestern blot analysis of PDCD7 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp39-rabbit-monoclonal-antibody-m06922-1-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06922-1-wb.jpgAnti-USP39 Rabbit Monoclonal AntibodyWestern blot analysis of USP39 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tug-rabbit-monoclonal-antibody-m05168-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05168-wb.jpgAnti-TUG Rabbit Monoclonal AntibodyWestern blot analysis of TUG expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cpsf73-rabbit-monoclonal-antibody-m08921-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08921-wb.jpgAnti-CPSF73 Rabbit Monoclonal AntibodyWestern blot analysis of CPSF73 expression in (1) Hela cell lysate; (2) NIH/3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08921-wb7.jpgAnti-CPSF73 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08921-wb8.jpgAnti-CPSF73 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cox6b1-rabbit-monoclonal-antibody-m10285-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10285-wb.jpgAnti-COX6B1 Rabbit Monoclonal AntibodyWestern blot analysis of COX6B1 expression in Caco 2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-srp19-rabbit-monoclonal-antibody-m07666-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07666-wb.jpgAnti-SRP19 Rabbit Monoclonal AntibodyWestern blot analysis of SRP19 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd84-rabbit-monoclonal-antibody-m04872-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04872-wb.jpgAnti-CD84 Rabbit Monoclonal AntibodyWestern blot analysis of CD84 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnph1-rabbit-monoclonal-antibody-m08676-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08676-wb7.jpgAnti-DNPH1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08676-wb8.jpgAnti-DNPH1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08676-wb.jpgAnti-DNPH1 Rabbit Monoclonal AntibodyWestern blot analysis of DNPH1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adenylate-kinase-4-rabbit-monoclonal-antibody-m07383-1-boster.html2026-03-17T05:12:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07383-1-wb.jpgAnti-Adenylate kinase 4 Rabbit Monoclonal AntibodyWestern blot analysis of Adenylate kinase 4 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-fgfr3-y724-rabbit-monoclonal-antibody-p00200-boster.html2026-04-03T05:00:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00200-wb.jpgAnti-Phospho-FGFR3 (Y724) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-FGFR3 (Y724) expression in (1) MCF7 treated with pervanadate cell lysate; (2) untreated. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il15ra-rabbit-monoclonal-antibody-m03016-1-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03016-1-wb.jpgAnti-IL15RA Rabbit Monoclonal AntibodyWestern blot analysis of IL15RA expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rasa1-rabbit-monoclonal-antibody-m01929-1-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01929-1-wb.jpgAnti-RASA1 Rabbit Monoclonal AntibodyWestern blot analysis of RASA1 expression in Rat brain cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-erbb2-y1139-rabbit-monoclonal-antibody-p00010-1-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00010-1-wb.jpgAnti-Phospho-ErbB2 (Y1139) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-ErbB2 (Y1139) expression in (1) A431 cell lysate; (2) A431 treated with EGF cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c1qbp-rabbit-monoclonal-antibody-m01439-1-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01439-1-c1qbp-primary-antibodies-wb-testing-1.jpgAnti-C1QBP Rabbit Monoclonal Antibody Western blot analysis of C1QBP using anti-C1QBP antibody (M01439-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse intestine tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1QBP antigen affinity purified monoclonal antibody (Catalog # M01439-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1QBP at approximately 36 kDa. The expected band size for C1QBP is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01439-1-c1qbp-primary-antibodies-ihc-testing-2.jpgAnti-C1QBP Rabbit Monoclonal Antibody IHC analysis of C1QBP using anti-C1QBP antibody (M01439-1). C1QBP was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-C1QBP Antibody (M01439-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01439-1-c1qbp-primary-antibodies-ihc-testing-3_1.jpgAnti-C1QBP Rabbit Monoclonal Antibody IHC analysis of C1QBP using anti-C1QBP antibody (M01439-1). C1QBP was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-C1QBP Antibody (M01439-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01439-1-c1qbp-primary-antibodies-ihc-testing-4_1.jpgAnti-C1QBP Rabbit Monoclonal Antibody IHC analysis of C1QBP using anti-C1QBP antibody (M01439-1). C1QBP was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-C1QBP Antibody (M01439-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-orp1-rabbit-monoclonal-antibody-m09676-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09676-wb.jpgAnti-ORP1 Rabbit Monoclonal AntibodyWestern blot analysis of ORP1 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cntn4-rabbit-monoclonal-antibody-m05452-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05452-wb.jpgAnti-CNTN4 Rabbit Monoclonal AntibodyWestern blot analysis of CNTN4 expression in Human cerebellum cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-heab-rabbit-monoclonal-antibody-m02473-1-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02473-1-wb.jpgAnti-HEAB Rabbit Monoclonal AntibodyWestern blot analysis of HEAB expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-elp4-rabbit-monoclonal-antibody-m07167-1-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07167-1-wb.jpgAnti-ELP4 Rabbit Monoclonal AntibodyWestern blot analysis of ELP4 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nctr2-rabbit-monoclonal-antibody-m05962-1-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05962-1-nctr2-primary-antibodies-wb-testing-1.jpgAnti-NCTR2 Rabbit Monoclonal AntibodyWestern blot analysis of NCTR2 using anti-NCTR2 antibody (M05962-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCTR2 antigen affinity purified monoclonal antibody (M05962-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCTR2 at approximately 44 kDa. The expected band size for NCTR2 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05962-1-ncr2-primary-antibodies-ihc-testing-1.jpgAnti-NCTR2 Rabbit Monoclonal AntibodyIHC analysis of CD336/NCR2 using anti-CD336/NCR2 antibody (M05962-1). CD336/NCR2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD336/NCR2 Antibody (M05962-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05962-1-ncr2-primary-antibodies-ihc-testing-2.jpgAnti-NCTR2 Rabbit Monoclonal AntibodyIHC analysis of CD336/NCR2 using anti-CD336/NCR2 antibody (M05962-1). CD336/NCR2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD336/NCR2 Antibody (M05962-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cap1-rabbit-monoclonal-antibody-m01531-boster.html2026-03-17T05:12:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01531-wb.jpgAnti-CAP1 Rabbit Monoclonal AntibodyWestern blot analysis of CAP1 expression in Raji cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01531-ip7.jpgAnti-CAP1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mc5-receptor-rabbit-monoclonal-antibody-m07019-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07019-wb.jpgAnti-MC5 Receptor Rabbit Monoclonal AntibodyWestern blot analysis of MC5 Receptor expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndrg3-rabbit-monoclonal-antibody-m10017-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10017-wb.jpgAnti-NDRG3 Rabbit Monoclonal AntibodyWestern blot analysis of NDRG3 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppp6c-rabbit-monoclonal-antibody-m05295-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05295-wb.jpgAnti-PPP6C Rabbit Monoclonal AntibodyWestern blot analysis of PPP6C expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hook2-rabbit-monoclonal-antibody-m08854-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08854-wb.jpgAnti-HOOK2 Rabbit Monoclonal AntibodyWestern blot analysis of HOOK2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-muta-rabbit-monoclonal-antibody-m34008-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m34008-mmut-primary-antibodies-wb-testing-1.jpgAnti-MUTA Rabbit Monoclonal Antibody Western blot analysis of MMUT using anti-MMUT antibody (M34008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMUT antigen affinity purified monoclonal antibody (Catalog # M34008) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMUT at approximately 83 kDa. The expected band size for MMUT is at 83 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clptm1-rabbit-monoclonal-antibody-m08423-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08423-wb.jpgAnti-CLPTM1 Rabbit Monoclonal AntibodyWestern blot analysis of CLPTM1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dpp3-rabbit-monoclonal-antibody-m07788-1-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07788-1-wb.jpgAnti-DPP3 Rabbit Monoclonal AntibodyWestern blot analysis of DPP3 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ttf1-rabbit-monoclonal-antibody-m01322-3-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01322-3-wb.jpgAnti-TTF1 Rabbit Monoclonal AntibodyWestern blot analysis of TTF1 expression in Rat lung cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01322-3-ttf-1-primary-antibodies-ihc-testing-1.jpgAnti-TTF1 Rabbit Monoclonal AntibodyIHC analysis of TTF-1 using anti-TTF-1 antibody (M01322-3). TTF-1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TTF-1 Antibody (M01322-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-agl-rabbit-monoclonal-antibody-m02555-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02555-wb.jpgAnti-AGL Rabbit Monoclonal AntibodyWestern blot analysis of AGL expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-samm50-rabbit-monoclonal-antibody-m06847-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06847-wb.jpgAnti-SAMM50 Rabbit Monoclonal AntibodyWestern blot analysis of SAMM50 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nop10-rabbit-monoclonal-antibody-m07394-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07394-nop10-primary-antibodies-wb-testing-1.jpgAnti-NOP10 Rabbit Monoclonal AntibodyWestern blot analysis of NOP10 using anti-NOP10 antibody (M07394). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human 293T whole cell lysates, Lane 8: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP10 antigen affinity purified monoclonal antibody (M07394) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOP10 at approximately 10 kDa. The expected band size for NOP10 is at 8 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-npy5r-rabbit-monoclonal-antibody-m06337-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06337-wb.jpgAnti-NPY5R Rabbit Monoclonal AntibodyWestern blot analysis of NPY5R expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dcaf7-rabbit-monoclonal-antibody-m08577-boster.html2026-03-17T05:12:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08577-dcaf7-primary-antibodies-wb-testing-1_1.jpgAnti-DCAF7 Rabbit Monoclonal Antibody Western blot analysis of DCAF7 using anti-DCAF7 antibody (M08577). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF7 antigen affinity purified monoclonal antibody (Catalog # M08577) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCAF7 at approximately 39 kDa. The expected band size for DCAF7 is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-mlkl-s345-rabbit-monoclonal-antibody-p00535-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00535-wb.jpgAnti-Phospho-MLKL (S345) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-MLKL (S345) expression in (1) L929 treated with Z-VAD + TNF alpha + SM164 cell lysate; (2) Untreated. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il11ra-rabbit-monoclonal-antibody-m06426-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06426-wb.jpgAnti-IL11RA Rabbit Monoclonal AntibodyWestern blot analysis of IL11RA expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06426-wb7.jpgAnti-IL11RA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06426-wb8.jpgAnti-IL11RA Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ptpn2-rabbit-monoclonal-antibody-m01597-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01597-wb.jpgAnti-PTPN2 Rabbit Monoclonal AntibodyWestern blot analysis of PTPN1/2 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01597-ip7.jpgAnti-PTPN2 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-itpa-rabbit-monoclonal-antibody-m02304-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02304-wb.jpgAnti-ITPA Rabbit Monoclonal AntibodyWestern blot analysis of ITPA expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ttf1-rabbit-monoclonal-antibody-m01322-4-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01322-4-wb.jpgAnti-TTF1 Rabbit Monoclonal AntibodyWestern blot analysis of TTF1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppm1e-rabbit-monoclonal-antibody-m08794-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08794-wb.jpgAnti-PPM1E Rabbit Monoclonal AntibodyWestern blot analysis of PPM1E expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sr-protein-repeat-rabbit-monoclonal-antibody-m04489-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04489-wb.jpgAnti-SR protein repeat Rabbit Monoclonal AntibodyWestern blot analysis of SR protein repeat expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chm-rabbit-monoclonal-antibody-m00814-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00814-wb.jpgAnti-CHM Rabbit Monoclonal AntibodyWestern blot analysis of CHM expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spermine-synthase-rabbit-monoclonal-antibody-m01831-1-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01831-1-wb7.jpgAnti-Spermine synthase Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01831-1-wb.jpgAnti-Spermine synthase Rabbit Monoclonal AntibodyWestern blot analysis of Spermine synthase expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01831-1-wb8.jpgAnti-Spermine synthase Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01831-1-wb9.jpgAnti-Spermine synthase Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-scin-rabbit-monoclonal-antibody-m07463-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07463-scin-primary-antibodies-wb-testing-1.jpgAnti-SCIN Rabbit Monoclonal Antibody Western blot analysis of SCIN using anti-SCIN antibody (M07463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCIN antigen affinity purified monoclonal antibody (M07463) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCIN at approximately 80 kDa. The expected band size for SCIN is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07463-wb8.jpgAnti-SCIN Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-chk2-t68-rabbit-monoclonal-antibody-p00277-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00277-1-wb.jpgAnti-Phospho-Chk2 (T68) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-Chk2 (T68) expression in (1) 293 treated with UV and Untreated cell lysate; (2) Untreated. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-taf15-rabbit-monoclonal-antibody-m03567-1-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-wb-testing-1_1.jpgAnti-TAF15 Rabbit Monoclonal Antibody Western blot analysis of TAF15 using anti-TAF15 antibody (M03567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF15 antigen affinity purified monoclonal antibody (Catalog # M03567-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF15 at approximately 75 kDa. The expected band size for TAF15 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-ihc-testing-2.jpgAnti-TAF15 Rabbit Monoclonal Antibody IHC analysis of TAF15 using anti-TAF15 antibody (M03567-1). TAF15 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TAF15 Antibody (M03567-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-ihc-testing-3.jpgAnti-TAF15 Rabbit Monoclonal Antibody IHC analysis of TAF15 using anti-TAF15 antibody (M03567-1). TAF15 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TAF15 Antibody (M03567-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-ihc-testing-4.jpgAnti-TAF15 Rabbit Monoclonal Antibody IHC analysis of TAF15 using anti-TAF15 antibody (M03567-1). TAF15 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TAF15 Antibody (M03567-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-ihc-testing-5.jpgAnti-TAF15 Rabbit Monoclonal Antibody IHC analysis of TAF15 using anti-TAF15 antibody (M03567-1). TAF15 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TAF15 Antibody (M03567-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-ihc-testing-6.jpgAnti-TAF15 Rabbit Monoclonal Antibody IHC analysis of TAF15 using anti-TAF15 antibody (M03567-1). TAF15 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TAF15 Antibody (M03567-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-ihc-testing-7.jpgAnti-TAF15 Rabbit Monoclonal Antibody IHC analysis of TAF15 using anti-TAF15 antibody (M03567-1). TAF15 was detected in a paraffin-embedded section of human cervix squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TAF15 Antibody (M03567-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-ihc-testing-8.jpgAnti-TAF15 Rabbit Monoclonal Antibody IHC analysis of TAF15 using anti-TAF15 antibody (M03567-1). TAF15 was detected in a paraffin-embedded section of diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TAF15 Antibody (M03567-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03567-1-taf15-primary-antibodies-if-testing-9.jpgAnti-TAF15 Rabbit Monoclonal Antibody IF analysis of TAF15 using anti-TAF15 antibody (M03567-1) and anti-Beta Tubulin antibody (M01857-3). TAF15 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-TAF15 Antibody (M03567-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c1s-rabbit-monoclonal-antibody-m02057-3-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02057-3-wb.jpgAnti-C1s Rabbit Monoclonal AntibodyWestern blot analysis of C1s expression in human plasma cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dpf2-rabbit-monoclonal-antibody-m07556-1-boster.html2026-03-17T05:12:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07556-1-wb.jpgAnti-DPF2 Rabbit Monoclonal AntibodyWestern blot analysis of DPF2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erp19-rabbit-monoclonal-antibody-m08975-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08975-erp19-primary-antibodies-wb-testing-1.jpgAnti-ERp19 Rabbit Monoclonal Antibody Western blot analysis of ERp19 using anti-ERp19 antibody (M08975). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp19 antigen affinity purified monoclonal antibody (Catalog # M08975) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp19 at approximately 17 kDa. The expected band size for ERp19 is at 19 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myl12b-rabbit-monoclonal-antibody-m10532-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10532-wb.jpgAnti-MYL12B Rabbit Monoclonal AntibodyWestern blot analysis of MYL12B expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mrps15-rabbit-monoclonal-antibody-m13979-1-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13979-1-wb.jpgAnti-MRPS15 Rabbit Monoclonal AntibodyWestern blot analysis of MRPS15 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cnap1-rabbit-monoclonal-antibody-m07277-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07277-wb.jpgAnti-CNAP1 Rabbit Monoclonal AntibodyWestern blot analysis of CNAP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-graf-rabbit-monoclonal-antibody-m08027-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08027-wb.jpgAnti-GRAF Rabbit Monoclonal AntibodyWestern blot analysis of GRAF expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adh4-rabbit-monoclonal-antibody-m04620-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04620-wb.jpgAnti-ADH4 Rabbit Monoclonal AntibodyWestern blot analysis of ADH4 expression in human liver cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uqcrh-rabbit-monoclonal-antibody-m09436-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09436-uqcrh-primary-antibodies-wb-testing-1.jpgAnti-UQCRH Rabbit Monoclonal Antibody Western blot analysis of UQCRH using anti-UQCRH antibody (M09436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UQCRH antigen affinity purified monoclonal antibody (Catalog # M09436) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UQCRH at approximately 13 kDa. The expected band size for UQCRH is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sod2-acetyl-k68-rabbit-monoclonal-antibody-m00349-2-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-2-sod2-primary-antibodies-wb-testing-1.jpgAnti-SOD2 (acetyl K68) Rabbit Monoclonal Antibody Western blot analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 (acetyl K68) antigen affinity purified monoclonal antibody (M00349-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2 (acetyl K68) at approximately 25 kDa. The expected band size for SOD2 (acetyl K68) is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-2-sod2-primary-antibodies-ihc-testing-2.jpgAnti-SOD2 (acetyl K68) Rabbit Monoclonal Antibody IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2). SOD2 (acetyl K68) was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit SOD2 (acetyl K68) Antibody (M00349-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-2-sod2-primary-antibodies-ihc-testing-3.jpgAnti-SOD2 (acetyl K68) Rabbit Monoclonal Antibody IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2). SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit SOD2 (acetyl K68) Antibody (M00349-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-2-sod2-primary-antibodies-ihc-testing-4.jpgAnti-SOD2 (acetyl K68) Rabbit Monoclonal Antibody IHC analysis of SOD2 (acetyl K68) using anti-SOD2 (acetyl K68) antibody (M00349-2). SOD2 (acetyl K68) was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit SOD2 (acetyl K68) Antibody (M00349-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-somatostatin-receptor-1-rabbit-monoclonal-antibody-m04717-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04717-wb.jpgAnti-Somatostatin Receptor 1 Rabbit Monoclonal AntibodyWestern blot analysis of Somatostatin Receptor 1 expression in 293T transfected with Somatostatin Receptor 1 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thromboxane-a2-receptor-rabbit-monoclonal-antibody-m01812-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01812-wb.jpgAnti-Thromboxane A2 receptor Rabbit Monoclonal AntibodyWestern blot analysis of Thromboxane A2 receptor expression in human platelet cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rpl13-rabbit-monoclonal-antibody-m06505-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06505-wb.jpgAnti-RPL13 Rabbit Monoclonal AntibodyWestern blot analysis of RPL13 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-syntaxin-16-rabbit-monoclonal-antibody-m06602-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06602-wb.jpgAnti-Syntaxin 16 Rabbit Monoclonal AntibodyWestern blot analysis of Syntaxin 16 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmut-rabbit-monoclonal-antibody-m34008-1-boster.html2026-03-17T05:12:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m34008-1-wb.jpgAnti-MMUT Rabbit Monoclonal AntibodyWestern blot analysis of MMUT expression in (1) HeLa cell lysate; (2) Mouse brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trpv5-rabbit-monoclonal-antibody-m03218-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-trpv5-primary-antibodies-wb-testing-1.jpgAnti-TRPV5 Rabbit Monoclonal AntibodyWestern blot analysis of TRPV5 using anti-TRPV5 antibody (M03218). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified monoclonal antibody (M03218) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 100 kDa. The expected band size for TRPV5 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-trpv5-primary-antibodies-ihc-testing-1.jpgAnti-TRPV5 Rabbit Monoclonal AntibodyIHC analysis of TRPV5 using anti-TRPV5 antibody (M03218). TRPV5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRPV5 Antibody (M03218) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-trpv5-primary-antibodies-ihc-testing-2.jpgAnti-TRPV5 Rabbit Monoclonal AntibodyIHC analysis of TRPV5 using anti-TRPV5 antibody (M03218). TRPV5 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRPV5 Antibody (M03218) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-trpv5-primary-antibodies-ihc-testing-3.jpgAnti-TRPV5 Rabbit Monoclonal AntibodyIHC analysis of TRPV5 using anti-TRPV5 antibody (M03218). TRPV5 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TRPV5 Antibody (M03218) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-12967_2025_6980_fig4_html.pngAnti-TRPV5 Rabbit Monoclonal AntibodyPHPT1 interacts with TRPV5, influencing spontaneous Ca 2+ activity and Ca current. A Co-immunoprecipitation analysis of TRPV5-PHPT1. B Upper panel: TRPV5 and KCNN4 phosphorylation-related protein sample analysis. Lower panel: Protein phosphorylation result quantifications (n = 6). C PHPT1 interaction with TRPV5 predicted using HDOCK ( ). Blue and pink represent TRPV5 and PHPT1, respectively, along with the 3D interaction image below. D Amino acid mutations in three TRPV5 variants. E PHPT1 expression and three TRPV5 mutations. Following the PHPT1 immunoprecipitation and TRPV5 protein mutations, TRPV5 and PHPT1 were assessed by immunoblotting. F Protein expression quantification (n = 6). G Left: Western blot results of changes in TRPV5 mutations on calcium pathway. The calcium pathway proteins include SERCA2, p -CaMK2, and CaMK2. The protein expression compared with that of the β-tubulin loading control. Right: Protein expression quantification. H Left panel: External membrane current activated by pulmonary artery smooth muscle cell depolarization. Right panel: I-V curve of the outgoing current. I Summary of the peak amplitudes as cytosolic Ca 2+ changes detected by Fluo-4 fluorescence (F/F0). Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-025-06980-8'>40877955</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-12967_2025_6980_fig5_html.pngAnti-TRPV5 Rabbit Monoclonal AntibodyTRPV5 inhibition suppresses PASMC proliferation and migration. A PASMCs were treated with RuR (0–5000 nmol/L) for 24 h, then assessed using a cell counting kit-8 under normoxic or hypoxic (1 % O 2 ) conditions with or without PHPT1-KD. The absorbance was measured at 450 nm (n = 6). B Left panel: PASMCs treated with RuR (0–5000 nmol/L) and exposed to normoxic or hypoxic conditions with or without PHPT1-KD for 48 h. Representative images of the wound closure area. Right panel: Wound closure area quantification (n = 6). C PASMCs were seeded into the upper chambers of Transwell plates, treated with RuR, and exposed to normoxic or hypoxic conditions with or without PHPT1-KD. Representative images of migrated cells. D Left panel: western blot analysis of TRPV5, KCNN4, CDKN1A, p-Akt, p-SMAD2, p-SMAD3, p-TGFβ, RAC1, and p-EGFR protein expression compared with that of the β-tubulin loading control in WT and PHPT1-KD PASMCs treated with RuR for 24 h. Right panel: Quantification of western blot results. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-025-06980-8'>40877955</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-12967_2025_6980_fig3_html.pngAnti-TRPV5 Rabbit Monoclonal AntibodyPHPT1-knockdown results in cardiopulmonary injury by disrupting ion binding and the PI3K/Akt pathway. A Left: Cell proliferation determination using a cell counting kit-8 (CCK-8) under normoxic or hypoxic (1 % O 2 ) conditions with or without PHPT1 KD, and absorbance measured at 450 nm. Right: CCK8 analysis quantification (n = 6). B Left: Cell proliferation determination using impedance detection under normoxic or hypoxic conditions with or without PHPT1 KD. Right: Impedance analysis quantification (n = 6). C Left: PASMCs were exposed to normoxic or hypoxic (1 % O 2 ) conditions with or without PHPT1 KD for 48 h. Right: Representative images of the wound closure area (scale bars, 50 μm). Right: Wound closure area quantification ( n = 6). D Left: PASMCs were seeded into the upper chamber of transwell plates and exposed to normoxic or hypoxic (1 % O 2 ) conditions with or without PHPT1 KD for 48 h. Right: Representative images of the migrated cells (scale bars, 500 μm). Right: The migrated cells were quantified by detecting crystal violet levels (n = 6). E Venn diagram of DEGs between WT and PHPT1-KD rat lung samples with or without hypoxia. F PCoA using the Bray–Curtis distance calculated based on the relative abundance of species. The 95% confdence ellipses are shown by circles. G Results of GO analysis. H Results of KEGG analysis. The significant KEGG sets in environmental information processing, organismal systems and human disease. I Heatmap showing the DEGs among CW, CP, MW and MP groups. J Top 20 genes with maximum abundance of MW or MP. K Results of GSEA analysis. L PCR verification of the RNA-seq results. M Up: Volcano plots of DEGs between WT and PHPT1-KD rat lungs; TRPV5 was significantly increased. Down: PCR verification of the RNA-seq results of TRPV5. N Up: Gene Ontology assignment of DEGs between WT and PHPT1-KD rat lungs exposed to hypoxia. Down: Kyoto Encyclopedia of Genes and Genomes pathway enrichment of DEGs between WT and PHPT1-KD rat lungs exposed to hypoxia. O Up: Heatmap showing the DEGs between WT and PHPT1-KD rat lungs; CDKN1A was significantly decreased. Down: PCR verification of the RNA-seq results of TRPV5 and CDKN1A. P Western blot analysis of TRPV5, KCNN4, CDKN1A, p-Akt, p-SMAD2, p-SMAD3, p-TGF-β, RAC1, and p-EGFR protein expression compared with that of the β-tubulin loading control in WT and PHPT1-KD rat lungs with or without hypoxia exposure. Q Western blot result quantifications (n = 6). The relative expression of each protein was normalized to that of β-tubulin. The results were obtained from three independent experiments and are represented as the mean ± SD. KD, knockdown; WT, wild type; DEGs, differentially expressed genes; RNA-seq, RNA sequencing; PASMCs, human pulmonary artery smooth muscle cells. * p < 0.05 relative to the control. # p < 0.05 relative to the hypoxia model group. CW, control; CP, PHPT1-KD; MW, hypoxia model; MP, hypoxia model with PHPT1-KD. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-025-06980-8'>40877955</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-12967_2025_6980_fig6_html.pngAnti-TRPV5 Rabbit Monoclonal AntibodyInhibition of PI3K suppresses the proliferation and migration of PASMCs. A PASMCs were treated with AS252424 (0–10 μmol/L) for 24 h and assessed using cell counting kit-8 under normoxic or hypoxic conditions with or without PHPT1-KD, and absorbance was measured at 450 nm ( n = 6). B Left panel: PASMCs were treated with AS252424 (0–10 μmol/L) and exposed to normoxic or hypoxic conditions with or without PHPT1-KD for 48 h. Representative images of the wound closure area. Right panel: Quantification of the wound closure area ( n = 6). C PASMCs were seeded into the upper chambers of transwell plates, treated with AS252424, and exposed to normoxic or hypoxic conditions with or without PHPT1-KD for 48 h. Representative images of migrated cells are shown. D Left panel: Western blot analysis of TRPV5, KCNN4, CDKN1A, p-Akt, p-SMAD2, p-SMAD3, p-TGFβ, RAC1, and p-EGFR protein expression compared with that of the β-tubulin loading control in WT and PHPT1-KD PASMCs treated with AS252424 for 24 h. Right panel: Western blot result quantifications. PASMCs, pulmonary artery smooth muscle cells. * p < 0.05 relative to the control. # p < 0.05 relative to the hypoxia model group. # p < 0.05 relative to the hypoxia with the PHPT1-KD group. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-025-06980-8'>40877955</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03218-12967_2025_6980_fig8_html.pngAnti-TRPV5 Rabbit Monoclonal AntibodyOverexpression of PHPT1 mitigates the proliferation and migration of PASMCs and upregulates TRPV5/p-PI3K/p-Akt expression under hypoxic conditions. A Western blot analysis of PHPT1 protein expression in PASMCs. Tubulin was used as a loading control. Quantification of western blot analysis from the upper panel. B Cell proliferation was determined using a cell counting kit-8 under normoxic or hypoxic conditions with or without PHPT1-OE, and absorbance was measured at 450 nm (n = 6). C Left panel: Cell proliferation was determined using impedance detection under normoxic or hypoxic conditions with or without PHPT1-OE. Right panel: Quantification of impedance analysis (n = 6). D Left panel: PASMCs were exposed to normoxic or hypoxic conditions with or without PHPT1-OE. Representative images of the wound closure area. Right panel: Quantification of the wound closure area (n = 6). E PASMCs were seeded into the upper chambers of Transwell plates and exposed to normoxic or hypoxic conditions with or without PHPT1-OE for 48 h. Representative images of migrated cells are shown. The migrated cells were quantified by detecting crystal violet levels (n = 6). F Left panel: External membrane current activated by depolarization of PASMCs. Right panel: The I-V curve of the outgoing current. G Summary of the peak amplitudes as cytosolic Ca 2+ changes detected by Fluo-4 fluorescence (F/F0). H Western blot analysis of TRPV5, KCNN4, CDKN1A, p-Akt, p-SMAD2, p-SMAD3, p-TGF-β, RAC1, and p-EGFR protein expression compared with that of the β-tubulin loading control in WT and PHPT1-OE rat lungs with or without hypoxia exposure. I Western blot result quantifications. The relative expression of each protein was normalized to that of β-tubulin. The results were obtained from three independent experiments and are represented as the mean ± SD. PASMCs, pulmonary artery smooth muscle cells; OE, overexpression. * p < 0.05 relative to the control. # p < 0.05 relative to the hypoxia model group. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-025-06980-8'>40877955</a> https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-snap29-rabbit-monoclonal-antibody-m05318-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05318-snap29-primary-antibodies-wb-testing-1.jpgAnti-SNAP29 Rabbit Monoclonal Antibody Western blot analysis of SNAP29 using anti-SNAP29 antibody (M05318). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP29 antigen affinity purified monoclonal antibody (M05318) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP29 at approximately 29 kDa. The expected band size for SNAP29 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05318-ip7.jpgAnti-SNAP29 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pkn2-rabbit-monoclonal-antibody-m04066-1-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04066-1-wb.jpgAnti-PKN2 Rabbit Monoclonal AntibodyWestern blot analysis of PKN2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kallikrein-8-rabbit-monoclonal-antibody-m03901-3-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03901-3-wb.jpgAnti-Kallikrein 8 Rabbit Monoclonal AntibodyWestern blot analysis of Kallikrein 8 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hars-rabbit-monoclonal-antibody-m05242-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05242-wb.jpgAnti-HARS Rabbit Monoclonal AntibodyWestern blot analysis of HARS expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adra1a-rabbit-monoclonal-antibody-m02110-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02110-adra1a-primary-antibodies-wb-testing-1.jpgAnti-ADRA1A Rabbit Monoclonal AntibodyWestern blot analysis of ADRA1A using anti-ADRA1A antibody (M02110). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA1A antigen affinity purified monoclonal antibody (M02110) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADRA1A at approximately 51 kDa. The expected band size for ADRA1A is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-enpp5-rabbit-monoclonal-antibody-m14956-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14956-wb.jpgAnti-ENPP5 Rabbit Monoclonal AntibodyWestern blot analysis of ENPP5 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calumenin-rabbit-monoclonal-antibody-m03193-1-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03193-1-calu-primary-antibodies-wb-testing-1.jpgAnti-Calumenin Rabbit Monoclonal AntibodyWestern blot analysis of CALU using anti-CALU antibody (M03193-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALU antigen affinity purified monoclonal antibody (M03193-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CALU at approximately 47 kDa. The expected band size for CALU is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03193-1-calu-primary-antibodies-ihc-testing-1.jpgAnti-Calumenin Rabbit Monoclonal AntibodyIHC analysis of CALU using anti-CALU antibody (M03193-1). CALU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CALU Antibody (M03193-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03193-1-calu-primary-antibodies-ihc-testing-2.jpgAnti-Calumenin Rabbit Monoclonal AntibodyIHC analysis of CALU using anti-CALU antibody (M03193-1). CALU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CALU Antibody (M03193-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03193-1-calu-primary-antibodies-ihc-testing-3.jpgAnti-Calumenin Rabbit Monoclonal AntibodyIHC analysis of CALU using anti-CALU antibody (M03193-1). CALU was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CALU Antibody (M03193-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03193-1-calu-primary-antibodies-ihc-testing-4.jpgAnti-Calumenin Rabbit Monoclonal AntibodyIHC analysis of CALU using anti-CALU antibody (M03193-1). CALU was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CALU Antibody (M03193-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-toe1-rabbit-monoclonal-antibody-m09852-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09852-wb.jpgAnti-TOE1 Rabbit Monoclonal AntibodyWestern blot analysis of TOE1 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldh16a1-rabbit-monoclonal-antibody-m14478-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14478-wb.jpgAnti-ALDH16A1 Rabbit Monoclonal AntibodyWestern blot analysis of ALDH16A1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rpl26l1-rabbit-monoclonal-antibody-m15759-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m15759-wb.jpgAnti-RPL26L1 Rabbit Monoclonal AntibodyWestern blot analysis of RPL26L1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calcipressin-1-rabbit-monoclonal-antibody-m02795-1-boster.html2026-03-17T05:12:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02795-1-wb.jpgAnti-Calcipressin 1 Rabbit Monoclonal AntibodyWestern blot analysis of Calcipressin 1 expression in mouse heart cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccdc98-rabbit-monoclonal-antibody-m32338-1-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32338-1-wb.jpgAnti-CCDC98 Rabbit Monoclonal AntibodyWestern blot analysis of CCDC98 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pcbd1-rabbit-monoclonal-antibody-m07264-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07264-wb.jpgAnti-PCBD1 Rabbit Monoclonal AntibodyWestern blot analysis of PCBD1 expression in Caco-2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sterol-carrier-protein-2-rabbit-monoclonal-antibody-m02947-1-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02947-1-scp2-primary-antibodies-wb-testing-1_1.jpgAnti-Sterol carrier protein 2 Rabbit Monoclonal Antibody Western blot analysis of Sterol carrier protein 2 using anti-Sterol carrier protein 2 antibody (M02947-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sterol carrier protein 2 antigen affinity purified monoclonal antibody (Catalog # M02947-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sterol carrier protein 2 at approximately 59 kDa. The expected band size for Sterol carrier protein 2 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02947-1-scp2-primary-antibodies-if-testing-2.jpgAnti-Sterol carrier protein 2 Rabbit Monoclonal Antibody IF analysis of Sterol carrier protein 2 using anti-Sterol carrier protein 2 antibody (M02947-1). Sterol carrier protein 2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-Sterol carrier protein 2 Antibody (M02947-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikzf3-rabbit-monoclonal-antibody-m01611-3-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01611-3-wb.jpgAnti-IKZF3 Rabbit Monoclonal AntibodyWestern blot analysis of IKZF3 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cops4-rabbit-monoclonal-antibody-m09585-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09585-wb.jpgAnti-COPS4 Rabbit Monoclonal AntibodyWestern blot analysis of COPS4 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-als2cr1-rabbit-monoclonal-antibody-m11760-2-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11760-2-wb.jpgAnti-ALS2CR1 Rabbit Monoclonal AntibodyWestern blot analysis of ALS2CR1 expression in LNCaP cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-tak1-s439-rabbit-monoclonal-antibody-p01458-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p01458-map3k7-primary-antibodies-wb-testing-1.jpgAnti-Phospho-TAK1 (S439) Rabbit Monoclonal Antibody Western blot analysis of Phospho-TAK1 using anti-Phospho-TAK1 antibody (P01458). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-TAK1 antigen affinity purified monoclonal antibody (Catalog # P01458) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospho-TAK1 at approximately 41 kDa. The expected band size for Phospho-TAK1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p01458-ip7.jpgAnti-Phospho-TAK1 (S439) Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il-1-beta-rabbit-monoclonal-antibody-m00101-3-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00101-3-wb.jpgAnti-IL-1 beta Rabbit Monoclonal AntibodyWestern blot analysis of IL1 beta expression in RAW 264.7 cell treated with Lipopolysaccharide (LPS) cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00101-3-il1b-primary-antibodies-wb-testing-1.jpgAnti-IL-1 beta Rabbit Monoclonal AntibodyWestern blot analysis of IL1B using anti-IL1B antibody (M00101-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: recombinant rat IL1B protein.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified monoclonal antibody (M00101-3) at 1:500 overnight at 4°C, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL1B at approximately 17 kDa. The expected band size for IL1B is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-p-glycoprotein-abcb1-picoband-trade-antibody-a00049-4-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-4-abcb1-primary-antibodies-wb-testing-1.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® Western blot analysis of P Glycoprotein/ABCB1 using anti-P Glycoprotein/ABCB1 antibody (A00049-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein/ABCB1 antigen affinity purified polyclonal antibody (Catalog # A00049-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein/ABCB1 at approximately 141 kDa. The expected band size for P Glycoprotein/ABCB1 is at 141 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-4-41598_2017_article_bfsrep42465_fig15_html.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband®Effects of the various tested formulations on P-gp expression in MCF-7/ADR cells. (1) Control; (2) Pluronic L61 unimers; (3) Curcumin; (4) mixed Pluronic L61/CUR solution; (5) F-pHSM; (6) F-pHSM-L61/CUR; (7) F-pHSM-L61; (8) F-pHSM/CUR. * P < 0.05: significantly different from cells treated with the blank medium, # P < 0.05: significantly different from the F-pHSM-treated cells. The blots were cropped and the full-length blots were included in the . Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep42465'>28195164</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-4-41598_2017_article_bfsrep42465_fig18_html.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband®Expression of P-gp and Cle-PARP in tumour tissue form mice after treatment with different formulations: 1. Saline; 2. DOX solution; 3. F-pHSM; 4. F-pHSM/CUR; 5. F-pHSM-L61; 6. F-pHSM-L61/CUR; 7. F-pHSM/CUR/DOX; 8. F-pHSM-L61/DOX; 9. F-pHSM-L61/CUR/DOX. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep42465'>28195164</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-4-abcb1-primary-antibodies-if-testing-2.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® IF analysis of P Glycoprotein/ABCB1 using anti-P Glycoprotein/ABCB1 antibody (A00049-4). P Glycoprotein/ABCB1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P Glycoprotein/ABCB1 Antibody (A00049-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-4-abcb1-primary-antibodies-fcm-testing-3_1.pngAnti-P Glycoprotein/ABCB1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-P Glycoprotein/ABCB1 antibody (A00049-4). Overlay histogram showing 293T cells stained with A00049-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein/ABCB1 Antibody (A00049-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atr-picoband-trade-antibody-a00262-2-boster.html2026-03-17T05:12:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00262-2-atr-primary-antibodies-if-testing-1.jpgAnti-ATR Antibody IF analysis of ATR using anti-ATR antibody (A00262-2). ATR was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATR Antibody (A00262-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-aurora-a-aurka-picoband-trade-antibody-a00246-3-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-3-aurka-primary-antibodies-wb-testing-1.jpgAnti-Aurora A/AURKA Antibody Picoband® Western blot analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora A/AURKA antigen affinity purified polyclonal antibody (Catalog # A00246-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora A/AURKA at approximately 50 kDa. The expected band size for Aurora A/AURKA is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-3-aurka-primary-antibodies-ihc-testing-2.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-3). Aurora A/AURKA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-3-aurka-primary-antibodies-ihc-testing-3.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-3). Aurora A/AURKA was detected in a paraffin-embedded section of human Hashimoto thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-3-aurka-primary-antibodies-if-testing-4.jpgAnti-Aurora A/AURKA Antibody Picoband® IF analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-3). Aurora A/AURKA was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Aurora A/AURKA Antibody (A00246-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-3-aurka-primary-antibodies-fcm-testing-5.pngAnti-Aurora A/AURKA Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-Aurora A/AURKA antibody (A00246-3). Overlay histogram showing HL-60 cells stained with A00246-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aurora A/AURKA Antibody (A00246-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-plunc-bpifa1-picoband-trade-antibody-a03162-2-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03162-2-bpifa1-primary-antibodies-wb-testing-1.jpgAnti-Plunc/BPIFA1 Antibody Picoband® Western blot analysis of Plunc/BPIFA1 using anti-Plunc/BPIFA1 antibody (A03162-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plunc/BPIFA1 antigen affinity purified polyclonal antibody (Catalog # A03162-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plunc/BPIFA1 at approximately 27 kDa. The expected band size for Plunc/BPIFA1 is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-bub1-picoband-trade-antibody-a00776-3-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00776-3-bub1-primary-antibodies-wb-testing-1.jpgAnti-BUB1 Antibody Picoband® Western blot analysis of BUB1 using anti-BUB1 antibody (A00776-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1 antigen affinity purified polyclonal antibody (Catalog # A00776-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB1 at approximately 130 kDa. The expected band size for BUB1 is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00776-3-bub1-primary-antibodies-ihc-testing-2.jpgAnti-BUB1 Antibody Picoband® IHC analysis of BUB1 using anti-BUB1 antibody (A00776-3). BUB1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BUB1 Antibody (A00776-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00776-3-bub1-primary-antibodies-ihc-testing-3.jpgAnti-BUB1 Antibody Picoband® IHC analysis of BUB1 using anti-BUB1 antibody (A00776-3). BUB1 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BUB1 Antibody (A00776-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00776-3-bub1-primary-antibodies-ihc-testing-4.jpgAnti-BUB1 Antibody Picoband® IHC analysis of BUB1 using anti-BUB1 antibody (A00776-3). BUB1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BUB1 Antibody (A00776-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cd2-picoband-trade-antibody-a00570-3-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00570-3-cd2-primary-antibodies-wb-testing-1.jpgAnti-CD2 Antibody Picoband®Western blot analysis of CD2 using anti-CD2 antibody (A00570-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2 antigen affinity purified polyclonal antibody (A00570-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD2 at approximately 48-55 kDa. The expected band size for CD2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00570-3-cd2-primary-antibodies-ihc-testing-1.jpgAnti-CD2 Antibody Picoband®IHC analysis of CD2 using anti-CD2 antibody (A00570-3). CD2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD2 Antibody (A00570-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00570-3-cd2-primary-antibodies-ip-testing-1_1.jpgAnti-CD2 Antibody Picoband®Immunoprecipitating (IP) CD2 in Jurkat whole cell lysate. Western blot analysis of CD2 using anti-CD2 antibody (A00570-3); Lane 1: Jurkat whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-CD2 antibody in Jurkat whole cell lysate; Lane 3: anti-CD2 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD2 antigen affinity purified polyclonal antibody (A00570-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CD2 at approximately 48 kDa. The expected band size for CD2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00570-3-cd2-primary-antibodies-fcm-testing-1.jpgAnti-CD2 Antibody Picoband®Flow Cytometry analysis of JK cells using anti-CD2 antibody (A00570-3). Overlay histogram showing JK cells stained with A00570-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD2 Antibody (A00570-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd93-picoband-trade-antibody-a04939-1-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-1.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-2.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-3.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-4.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-5.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-6.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-7.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-8.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-ihc-testing-9.jpgAnti-CD93 Antibody IHC analysis of CD93 using anti-CD93 antibody (A04939-1). CD93 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD93 Antibody (A04939-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-fcm-testing-10.pngAnti-CD93 Antibody Flow Cytometry analysis of THP-1 cells using anti-CD93 antibody (A04939-1). Overlay histogram showing THP-1 cells stained with A04939-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD93 Antibody (A04939-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04939-1-cd93-primary-antibodies-fcm-testing-11.pngAnti-CD93 Antibody Flow Cytometry analysis of U937 cells using anti-CD93 antibody (A04939-1). Overlay histogram showing U937 cells stained with A04939-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD93 Antibody (A04939-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-p-cadherin-cdh3-picoband-trade-antibody-a03353-2-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03353-2-cdh3-primary-antibodies-wb-testing-1.jpgAnti-P cadherin/CDH3 Antibody Picoband®Western blot analysis of P Cadherin/CDH3 using anti-P Cadherin/CDH3 antibody (A03353-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Cadherin/CDH3 antigen affinity purified polyclonal antibody (Catalog # A03353-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P Cadherin/CDH3 at approximately 120 kDa. The expected band size for P Cadherin/CDH3 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03353-2-cdh3-primary-antibodies-if-testing-1.jpgAnti-P cadherin/CDH3 Antibody Picoband®IF analysis of P cadherin/CDH3 using anti-P cadherin/CDH3 antibody (A03353-2). P cadherin/CDH3 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P cadherin/CDH3 Antibody (A03353-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03353-2-cdh3-primary-antibodies-fcm-testing-1_1.jpgAnti-P cadherin/CDH3 Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-P cadherin/CDH3 antibody (A03353-2). Overlay histogram showing A431 cells stained with A03353-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P cadherin/CDH3 Antibody (A03353-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cdt1-dup-picoband-trade-antibody-a01035-1-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01035-1-cdt1-primary-antibodies-wb-testing-1_1.jpgAnti-CDT1/DUP Antibody Picoband® Western blot analysis of CDT1/DUP using anti-CDT1/DUP antibody (A01035-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1/DUP antigen affinity purified polyclonal antibody (Catalog # A01035-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDT1/DUP at approximately 60 kDa. The expected band size for CDT1/DUP is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01035-1-cdt1-primary-antibodies-if-testing-2.jpgAnti-CDT1/DUP Antibody Picoband® IF analysis of CDT1/DUP using anti-CDT1/DUP antibody (A01035-1) and anti-Beta Tubulin antibody (M01857-3). CDT1/DUP was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CDT1/DUP Antibody (A01035-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Donkey Anti-Rabbit IgG (BA1146) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01035-1-cdt1-primary-antibodies-fcm-testing-3.jpgAnti-CDT1/DUP Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-CDT1/DUP antibody (A01035-1). Overlay histogram showing 293T cells stained with A01035-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDT1/DUP Antibody (A01035-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-e2f3-picoband-trade-antibody-a03068-3-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03068-3-e2f3-primary-antibodies-wb-testing-1.jpgAnti-E2F3 Antibody Picoband® Western blot analysis of E2F3 using anti-E2F3 antibody (A03068-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F3 antigen affinity purified polyclonal antibody (Catalog # A03068-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F3 at approximately 49 kDa. The expected band size for E2F3 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03068-3-e2f3-primary-antibodies-fcm-testing-2.pngAnti-E2F3 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-E2F3 antibody (A03068-3). Overlay histogram showing HL-60 cells stained with A03068-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-E2F3 Antibody (A03068-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sgt1-ecd-picoband-trade-antibody-a00567-2-boster.html2026-03-17T05:12:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00567-2-ecd-primary-antibodies-wb-testing-1_1.jpgAnti-SGT1/ECD Antibody Picoband® Western blot analysis of SGT1/ECD using anti-SGT1/ECD antibody (A00567-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGT1/ECD antigen affinity purified polyclonal antibody (Catalog # A00567-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SGT1/ECD at approximately 73 kDa. The expected band size for SGT1/ECD is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00567-2-ecd-primary-antibodies-if-testing-2_1.jpgAnti-SGT1/ECD Antibody Picoband® IF analysis of SGT1/ECD using anti-SGT1/ECD antibody (A00567-2) and anti-Tubulin Alpha antibody (M03989-3). SGT1/ECD was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SGT1/ECD Antibody (A00567-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00567-2-ecd-primary-antibodies-ip-testing-3.jpgAnti-SGT1/ECD Antibody Picoband® Immunoprecipitating (IP) SGT1/ECD in MCF-7 whole cell lysate. Western blot analysis of SGT1/ECD using anti-SGT1/ECD antibody (A00567-2); Lane 1: MCF-7 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SGT1/ECD antibody in MCF-7 whole cell lysate; Lane 3: anti-SGT1/ECD antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SGT1/ECD antigen affinity purified polyclonal antibody (A00567-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SGT1/ECD at approximately 73 kDa. The expected band size for SGT1/ECD is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00567-2-ecd-primary-antibodies-fcm-testing-4.jpgAnti-SGT1/ECD Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SGT1/ECD antibody (A00567-2). Overlay histogram showing K562 cells stained with A00567-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGT1/ECD Antibody (A00567-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-enah-mena-picoband-trade-antibody-a05337-2-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05337-2-enah-primary-antibodies-wb-testing-1.jpgAnti-ENAH/MENA Antibody Picoband® Western blot analysis of ENAH/MENA using anti-ENAH/MENA antibody (A05337-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENAH/MENA antigen affinity purified polyclonal antibody (Catalog # A05337-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENAH/MENA at approximately 88 kDa. The expected band size for ENAH/MENA is at 67 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05337-2-enah-primary-antibodies-ihc-testing-2.pngAnti-ENAH/MENA Antibody Picoband® IHC analysis of ENAH/MENA using anti-ENAH/MENA antibody (A05337-2). ENAH/MENA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENAH/MENA Antibody (A05337-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05337-2-enah-primary-antibodies-ihc-testing-3.pngAnti-ENAH/MENA Antibody Picoband® IHC analysis of ENAH/MENA using anti-ENAH/MENA antibody (A05337-2). ENAH/MENA was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENAH/MENA Antibody (A05337-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05337-2-enah-primary-antibodies-ihc-testing-4.pngAnti-ENAH/MENA Antibody Picoband® IHC analysis of ENAH/MENA using anti-ENAH/MENA antibody (A05337-2). ENAH/MENA was detected in a paraffin-embedded section of human the renal pelvis is squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENAH/MENA Antibody (A05337-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05337-2-enah-primary-antibodies-if-testing-5.jpgAnti-ENAH/MENA Antibody Picoband® IF analysis of ENAH/MENA using anti-ENAH/MENA antibody (A05337-2). ENAH/MENA was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ENAH/MENA Antibody (A05337-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fgfr3-picoband-trade-antibody-a00200-3-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00200-3-fgfr3-primary-antibodies-wb-testing-1.jpgAnti-FGFR3 Antibody Picoband® Western blot analysis of FGFR3 using anti-FGFR3 antibody (A00200-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR3 antigen affinity purified polyclonal antibody (Catalog # A00200-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR3 at approximately 100 kDa. The expected band size for FGFR3 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00200-3-fgfr3-primary-antibodies-if-testing-2.jpgAnti-FGFR3 Antibody Picoband® IF analysis of FGFR3 using anti-FGFR3 antibody (A00200-3). FGFR3 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FGFR3 Antibody (A00200-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00200-3-fgfr3-primary-antibodies-fcm-testing-3.pngAnti-FGFR3 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-FGFR3 antibody (A00200-3). Overlay histogram showing 293T cells stained with A00200-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGFR3 Antibody (A00200-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gata1-picoband-trade-antibody-a00408-3-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00408-3-gata1-primary-antibodies-wb-testing-1.jpgAnti-GATA1 Antibody Picoband® Western blot analysis of GATA1 using anti-GATA1 antibody (A00408-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1 antigen affinity purified polyclonal antibody (Catalog # A00408-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA1 at approximately 43-50 kDa. The expected band size for GATA1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00408-3-gata1-primary-antibodies-ihc-testing-2.jpgAnti-GATA1 Antibody Picoband® IHC analysis of GATA1 using anti-GATA1 antibody (A00408-3). GATA1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GATA1 Antibody (A00408-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00408-3-gata1-primary-antibodies-fcm-testing-3.pngAnti-GATA1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-GATA1 antibody (A00408-3). Overlay histogram showing K562 cells stained with A00408-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GATA1 Antibody (A00408-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gata6-picoband-trade-antibody-a00778-3-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00778-3-gata6-primary-antibodies-wb-testing-1.jpgAnti-GATA6 Antibody Picoband® Western blot analysis of GATA6 using anti-GATA6 antibody (A00778-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA6 antigen affinity purified polyclonal antibody (Catalog # A00778-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA6 at approximately 65 kDa. The expected band size for GATA6 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00778-3-gata6-primary-antibodies-if-testing-2.jpgAnti-GATA6 Antibody Picoband® IF analysis of GATA6 using anti-GATA6 antibody (A00778-3). GATA6 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GATA6 Antibody (A00778-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00778-3-gata6-primary-antibodies-if-testing-3.jpgAnti-GATA6 Antibody Picoband® IF analysis of GATA6 using anti-GATA6 antibody (A00778-3). GATA6 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GATA6 Antibody (A00778-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00778-3-gata6-primary-antibodies-fcm-testing-4.pngAnti-GATA6 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-GATA6 antibody (A00778-3). Overlay histogram showing U20S cells stained with A00778-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GATA6 Antibody (A00778-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gjb2-picoband-trade-antibody-a00512-1-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00512-1-gjb2-primary-antibodies-wb-testing-1.jpgAnti-GJB2 Antibody Picoband® Western blot analysis of GJB2 using anti-GJB2 antibody (A00512-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJB2 antigen affinity purified polyclonal antibody (Catalog # A00512-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJB2 at approximately 26 kDa. The expected band size for GJB2 is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-giantin-golgb1-picoband-trade-antibody-a08226-1-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-wb-testing-1.jpgAnti-Giantin/GOLGB1 Antibody Picoband® Western blot analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Giantin/GOLGB1 antigen affinity purified polyclonal antibody (Catalog # A08226-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Giantin/GOLGB1 at approximately 376 kDa. The expected band size for Giantin/GOLGB1 is at 376 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-ihc-testing-2.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IHC analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-ihc-testing-3.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IHC analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-ihc-testing-4.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IHC analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-ihc-testing-5.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IHC analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-ihc-testing-6.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IHC analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-ihc-testing-7.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IHC analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-ihc-testing-8.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IHC analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-if-testing-9.jpgAnti-Giantin/GOLGB1 Antibody Picoband® IF analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Giantin/GOLGB1 was detected in an immunocytochemical section of RT4 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Giantin/GOLGB1 Antibody (A08226-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08226-1-golgb1-primary-antibodies-fcm-testing-10.pngAnti-Giantin/GOLGB1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Giantin/GOLGB1 antibody (A08226-1). Overlay histogram showing HepG2 cells stained with A08226-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Giantin/GOLGB1 Antibody (A08226-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hemoglobin-hba1-hba2-picoband-trade-antibody-a00233-2-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00233-2-hab1-hab2-primary-antibodies-wb-testing-1.jpgAnti-Hemoglobin/HBA1/HBA2 Antibody Picoband® Western blot analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemoglobin/HBA1/HBA2 antigen affinity purified polyclonal antibody (Catalog # A00233-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemoglobin/HBA1/HBA2 at approximately 15 kDa. The expected band size for Hemoglobin/HBA1/HBA2 is at 15 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00233-2-hab1-hab2-primary-antibodies-ihc-testing-2.jpgAnti-Hemoglobin/HBA1/HBA2 Antibody Picoband® IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2). Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00233-2-hab1-hab2-primary-antibodies-ihc-testing-3.jpgAnti-Hemoglobin/HBA1/HBA2 Antibody Picoband® IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2). Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00233-2-hab1-hab2-primary-antibodies-ihc-testing-4_1.jpgAnti-Hemoglobin/HBA1/HBA2 Antibody Picoband® IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2). Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00233-2-hab1-hab2-primary-antibodies-ihc-testing-5.jpgAnti-Hemoglobin/HBA1/HBA2 Antibody Picoband® IHC analysis of Hemoglobin/HBA1/HBA2 using anti-Hemoglobin/HBA1/HBA2 antibody (A00233-2). Hemoglobin/HBA1/HBA2 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hemoglobin/HBA1/HBA2 Antibody (A00233-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-hdac8-picoband-trade-antibody-a01843-1-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01843-1-hdac8-primary-antibodies-wb-testing-1.jpgAnti-HDAC8 Antibody Picoband® Western blot analysis of HDAC8 using anti-HDAC8 antibody (A01843-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 antigen affinity purified polyclonal antibody (Catalog # A01843-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8 at approximately 50 kDa. The expected band size for HDAC8 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-l1cam-picoband-trade-antibody-a00729-3-boster.html2026-03-17T05:12:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00729-3-l1cam-primary-antibodies-wb-testing-1.jpgAnti-L1CAM Antibody Picoband® Western blot analysis of L1CAM using anti-L1CAM antibody (A00729-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L1CAM antigen affinity purified polyclonal antibody (Catalog # A00729-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L1CAM at approximately 250 kDa. The expected band size for L1CAM is at 140 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-lpo-picoband-trade-antibody-a01483-2-boster.html2026-03-17T05:12:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01483-2-lpo-primary-antibodies-wb-testing-1.jpgAnti-LPO Antibody Picoband® Western blot analysis of LPO using anti-LPO antibody (A01483-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPO antigen affinity purified polyclonal antibody (Catalog # A01483-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPO at approximately 80 kDa. The expected band size for LPO is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01483-2-lpo-primary-antibodies-if-testing-2.jpgAnti-LPO Antibody Picoband® IF analysis of LPO using anti-LPO antibody (A01483-2). LPO was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LPO Antibody (A01483-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tau-mapt-picoband-trade-antibody-a00097-3-boster.html2026-03-17T05:12:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00097-3-mapt-primary-antibodies-wb-testing-1.jpgAnti-Tau/MAPT Antibody Picoband® Western blot analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tau/MAPT antigen affinity purified polyclonal antibody (Catalog # A00097-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tau/MAPT at approximately 50-70 kDa. The expected band size for Tau/MAPT is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00097-3-mapt-primary-antibodies-ihc-testing-1.jpgAnti-Tau/MAPT Antibody Picoband®IHC analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Tau/MAPT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tau/MAPT Antibody (A00097-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00097-3-mapt-primary-antibodies-if-testing-2.jpgAnti-Tau/MAPT Antibody Picoband® IF analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Tau/MAPT was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tau/MAPT Antibody (A00097-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00097-3-mapt-primary-antibodies-ihc-testing-2.jpgAnti-Tau/MAPT Antibody Picoband®IHC analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Tau/MAPT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tau/MAPT Antibody (A00097-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00097-3-mapt-primary-antibodies-ihc-testing-3.jpgAnti-Tau/MAPT Antibody Picoband®IHC analysis of Tau/MAPT using anti-Tau/MAPT antibody (A00097-3). Tau/MAPT was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tau/MAPT Antibody (A00097-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-msl2-picoband-trade-antibody-a02712-1-boster.html2026-03-17T05:12:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02712-1-msl2-primary-antibodies-wb-testing-1.jpgAnti-MSL2 Antibody Picoband® Western blot analysis of MSL2 using anti-MSL2 antibody (A02712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL2 antigen affinity purified polyclonal antibody (Catalog # A02712-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL2 at approximately 63 kDa. The expected band size for MSL2 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02712-1-msl2-primary-antibodies-if-testing-2.jpgAnti-MSL2 Antibody Picoband® IF analysis of MSL2 using anti-MSL2 antibody (A02712-1). MSL2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MSL2 Antibody (A02712-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02712-1-msl2-primary-antibodies-fcm-testing-3.pngAnti-MSL2 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-MSL2 antibody (A02712-1). Overlay histogram showing K562 cells stained with A02712-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSL2 Antibody (A02712-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mthfd1l-picoband-trade-antibody-a06289-1-boster.html2026-03-17T05:12:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06289-1-mthfd1l-primary-antibodies-wb-testing-1.jpgAnti-MTHFD1L Antibody Picoband® Western blot analysis of MTHFD1L using anti-MTHFD1L antibody (A06289-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD1L antigen affinity purified polyclonal antibody (Catalog # A06289-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTHFD1L at approximately 106 kDa. The expected band size for MTHFD1L is at 106 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06289-1-mthfd1l-primary-antibodies-ihc-testing-1.jpgAnti-MTHFD1L Antibody Picoband®IHC analysis of MTHFD1L using anti-MTHFD1L antibody (A06289-1). MTHFD1L was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1L Antibody (A06289-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06289-1-mthfd1l-primary-antibodies-if-testing-2.jpgAnti-MTHFD1L Antibody Picoband® IF analysis of MTHFD1L using anti-MTHFD1L antibody (A06289-1). MTHFD1L was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MTHFD1L Antibody (A06289-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06289-1-mthfd1l-primary-antibodies-fcm-testing-3.pngAnti-MTHFD1L Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-MTHFD1L antibody (A06289-1). Overlay histogram showing HEL cells stained with A06289-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTHFD1L Antibody (A06289-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mus81-picoband-trade-antibody-a00810-2-boster.html2026-03-17T05:12:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00810-2-mus81-primary-antibodies-wb-testing-1.jpgAnti-MUS81 Antibody Picoband® Western blot analysis of MUS81 using anti-MUS81 antibody (A00810-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUS81 antigen affinity purified polyclonal antibody (Catalog # A00810-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUS81 at approximately 52-72 kDa. The expected band size for MUS81 is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-myh6-picoband-trade-antibody-a02808-2-boster.html2026-03-17T05:12:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02808-2-myh6-primary-antibodies-wb-testing-1.jpgAnti-MYH6 Antibody Picoband® Western blot analysis of MYH6 using anti-MYH6 antibody (A02808-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH6 antigen affinity purified polyclonal antibody (Catalog # A02808-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH6 at approximately 224 kDa. The expected band size for MYH6 is at 224 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02808-2-myh6-primary-antibodies-ihc-testing-2.jpgAnti-MYH6 Antibody Picoband® IHC analysis of MYH6 using anti-MYH6 antibody (A02808-2). MYH6 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYH6 Antibody (A02808-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02808-2-myh6-primary-antibodies-ihc-testing-3.jpgAnti-MYH6 Antibody Picoband® IHC analysis of MYH6 using anti-MYH6 antibody (A02808-2). MYH6 was detected in a paraffin-embedded section of mouse cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYH6 Antibody (A02808-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02808-2-myh6-primary-antibodies-ihc-testing-4.jpgAnti-MYH6 Antibody Picoband® IHC analysis of MYH6 using anti-MYH6 antibody (A02808-2). MYH6 was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYH6 Antibody (A02808-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02808-2-myh6-primary-antibodies-fcm-testing-5.pngAnti-MYH6 Antibody Picoband®Figure 5 Flow Cytometry analysis of U937 cells using anti-MYH6 antibody (A02808-2). Overlay histogram showing U937 cells stained with A02808-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYH6 Antibody (A02808-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nac1-nacc1-picoband-trade-antibody-a08675-3-boster.html2026-03-17T05:12:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08675-3-nacc1-primary-antibodies-wb-testing-1.jpgAnti-Nac1/NACC1 Antibody Picoband® Western blot analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (A08675-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nac1/NACC1 antigen affinity purified polyclonal antibody (Catalog # A08675-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nac1/NACC1 at approximately 68 kDa. The expected band size for Nac1/NACC1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08675-3-nacc1-primary-antibodies-ihc-testing-2.jpgAnti-Nac1/NACC1 Antibody Picoband® IHC analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (A08675-3). Nac1/NACC1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nac1/NACC1 Antibody (A08675-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08675-3-nacc1-primary-antibodies-ihc-testing-3.jpgAnti-Nac1/NACC1 Antibody Picoband® IHC analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (A08675-3). Nac1/NACC1 was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nac1/NACC1 Antibody (A08675-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08675-3-nacc1-primary-antibodies-ihc-testing-4.jpgAnti-Nac1/NACC1 Antibody Picoband® IHC analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (A08675-3). Nac1/NACC1 was detected in a paraffin-embedded section of human the renal pelvis is squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nac1/NACC1 Antibody (A08675-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08675-3-nacc1-primary-antibodies-if-testing-5.jpgAnti-Nac1/NACC1 Antibody Picoband® IF analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (A08675-3). Nac1/NACC1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Nac1/NACC1 Antibody (A08675-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 555 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08675-3-nacc1-primary-antibodies-fcm-testing-6.pngAnti-Nac1/NACC1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-Nac1/NACC1 antibody (A08675-3). Overlay histogram showing K562 cells stained with A08675-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nac1/NACC1 Antibody (A08675-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ngf-picoband-trade-antibody-a00341-3-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00341-3-ngf-primary-antibodies-wb-testing-1.jpgAnti-NGF Antibody Picoband® Western blot analysis of NGF using anti-NGF antibody (A00341-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF antigen affinity purified polyclonal antibody (Catalog # A00341-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF at approximately 27 kDa. The expected band size for NGF is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-nnos-neuronal-nos1-picoband-trade-antibody-a01070-2-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01070-2-nos1-primary-antibodies-wb-testing-1.jpgAnti-nNOS (neuronal)/NOS1 Antibody Picoband® Western blot analysis of nNOS (neuronal)/NOS1 using anti-nNOS (neuronal)/NOS1 antibody (A01070-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nNOS (neuronal)/NOS1 antigen affinity purified polyclonal antibody (Catalog # A01070-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nNOS (neuronal)/NOS1 at approximately 161 kDa. The expected band size for nNOS (neuronal)/NOS1 is at 161 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01070-2-41598_2018_23568_fig7_html.jpgAnti-nNOS (neuronal)/NOS1 Antibody Picoband®Effect of exercise on alcohol induced neuronal damage. ( a,b ) Representative western blot analysis showing the levels of neuronal proteins (NeuN and NSC) in different mice groups ( a ). Histogram showing the quantitative estimation of nNOS and NSE proteins after normalization with GAPDH (b). ( c,d ) Representative images showing coronal slices of mice brains stained with cresyl violet (40× magnification) ( c ). Scatter dot plot showing the number of cresyl violet positive cells in different groups of mice ( d ). ( e,f ) Representative images showing Fluoro-Jade C (FJC) staining in brain sections of the different groups of mice (10× magnification). A marked decrease of FJC-stained degenerating neurons (arrows) were observed in CT, EX and AL+EX groups, indicating a lesser degree of neuronal cell death. Brain sections of AL treated mice showing a greater number of FJC-positive neurons (arrows), reflecting increased neuronal cell death ( e ). Scatter dot plot showing the numbers of degenerating neurons in different experimental mice groups ( f ). All the data are represented as mean values ± standard error (SE) in 5 independent experiments. * ,# p < 0.05 considered significant. *p < 0.05 vs. CT and # p < 0.05 vs. AL group. Uncropped blots for a are presented in Supplementary Fig. . Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-23568-z'>29581524</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01070-2-nos1-primary-antibodies-fcm-testing-2.pngAnti-nNOS (neuronal)/NOS1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-nNOS (neuronal)/NOS1 antibody (A01070-2). Overlay histogram showing U87 cells stained with A01070-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-nNOS (neuronal)/NOS1 Antibody (A01070-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pdgf-aa-pdgfa-picoband-trade-antibody-a02257-1-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02257-1-pdgfa-primary-antibodies-wb-testing-1.jpgAnti-PDGF AA/PDGFA Antibody Picoband® Western blot analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody (A02257-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGF AA/PDGFA antigen affinity purified polyclonal antibody (Catalog # A02257-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGF AA/PDGFA at approximately 24 kDa. The expected band size for PDGF AA/PDGFA is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02257-1-pdgfa-primary-antibodies-if-testing-2.jpgAnti-PDGF AA/PDGFA Antibody Picoband® IF analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody (A02257-1). PDGF AA/PDGFA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDGF AA/PDGFA Antibody (A02257-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-pdgfc-picoband-trade-antibody-a03092-2-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03092-2-pdgfc-primary-antibodies-wb-testing-1.jpgAnti-PDGFC Antibody Picoband® Western blot analysis of PDGFC using anti-PDGFC antibody (A03092-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFC antigen affinity purified polyclonal antibody (Catalog # A03092-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFC at approximately 39 kDa. The expected band size for PDGFC is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03092-2-pdgfc-primary-antibodies-fcm-testing-2.pngAnti-PDGFC Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-PDGFC antibody (A03092-2). Overlay histogram showing U251 cells stained with A03092-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDGFC Antibody (A03092-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pdp1-pdp-picoband-trade-antibody-a05607-2-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05607-2-pdp1-primary-antibodies-wb-testing-1.jpgAnti-PDP1/PDP Antibody Picoband® Western blot analysis of PDP1/PDP using anti-PDP1/PDP antibody (A05607-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDP1/PDP antigen affinity purified polyclonal antibody (Catalog # A05607-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDP1/PDP at approximately 53-61 kDa. The expected band size for PDP1/PDP is at 61 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05607-2-pdp1-primary-antibodies-if-testing-2.jpgAnti-PDP1/PDP Antibody Picoband® IF analysis of PDP1/PDP using anti-PDP1/PDP antibody (A05607-2). PDP1/PDP was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDP1/PDP Antibody (A05607-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05607-2-pdp1-primary-antibodies-fcm-testing-3.pngAnti-PDP1/PDP Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-PDP1/PDP antibody (A05607-2). Overlay histogram showing U937 cells stained with A05607-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDP1/PDP Antibody (A05607-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-phospholamban-pln-picoband-trade-antibody-a01395-1-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01395-1-pln-primary-antibodies-wb-testing-1.jpgAnti-Phospholamban/PLN Antibody Picoband® Western blot analysis of Phospholamban/PLN using anti-Phospholamban/PLN antibody (A01395-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospholamban/PLN antigen affinity purified polyclonal antibody (Catalog # A01395-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Phospholamban/PLN at approximately 23 kDa. The expected band size for Phospholamban/PLN is at 6 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01395-1-pln-primary-antibodies-ihc-testing-1.jpgAnti-Phospholamban/PLN Antibody Picoband®IHC analysis of Phospholamban/PLN using anti-Phospholamban/PLN antibody (A01395-1). Phospholamban/PLN was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Phospholamban/PLN Antibody (A01395-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01395-1-pln-primary-antibodies-ihc-testing-4.jpgAnti-Phospholamban/PLN Antibody Picoband®IHC analysis of PLB/PLN using anti-PLB/PLN antibody (A01395-1). PLB/PLN was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLB/PLN Antibody (A01395-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01395-1-pln-primary-antibodies-ihc-testing-2.jpgAnti-Phospholamban/PLN Antibody Picoband® IHC analysis of Phospholamban/PLN using anti-Phospholamban/PLN antibody (A01395-1). Phospholamban/PLN was detected in a paraffin-embedded section of mouse cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Phospholamban/PLN Antibody (A01395-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01395-1-pln-primary-antibodies-ihc-testing-3.jpgAnti-Phospholamban/PLN Antibody Picoband® IHC analysis of Phospholamban/PLN using anti-Phospholamban/PLN antibody (A01395-1). Phospholamban/PLN was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Phospholamban/PLN Antibody (A01395-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-pola2-picoband-trade-antibody-a08427-2-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08427-2-pola2-primary-antibodies-wb-testing-1.jpgAnti-POLA2 Antibody Picoband® Western blot analysis of POLA2 using anti-POLA2 antibody (A08427-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLA2 antigen affinity purified polyclonal antibody (Catalog # A08427-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLA2 at approximately 70 kDa. The expected band size for POLA2 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08427-2-pola2-primary-antibodies-fcm-testing-2.pngAnti-POLA2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-POLA2 antibody (A08427-2). Overlay histogram showing U251 cells stained with A08427-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLA2 Antibody (A08427-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oct4-pou5f1-picoband-trade-antibody-a00174-3-boster.html2026-03-17T05:12:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00174-3-pou5f1-primary-antibodies-wb-testing-1.jpgAnti-Oct4/POU5F1 Antibody Picoband® Western blot analysis of Oct4/POU5F1 using anti-Oct4/POU5F1 antibody (A00174-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oct4/POU5F1 antigen affinity purified polyclonal antibody (Catalog # A00174-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oct4/POU5F1 at approximately 39-45 kDa. The expected band size for Oct4/POU5F1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00174-3-13287_2019_1544_fig1_html.pngAnti-Oct4/POU5F1 Antibody Picoband®Effect of IGF-1 overexpression on BMSCs. a Identification of BMSCs by flow cytometry analysis. b Supernatants from cultured BMSCs-NC and BMSCs-IGF-1 were collected and subjected to ELISA to determine IGF-1 levels. c Cells were exposed to hypoxia for 48 h, and cell proliferation was determined by MTS assay. d Apoptosis was determined by TUNEL assay. e Cell migration was determined by Transwell assay. f Expression of OCT4, NANOG, cleaved caspase-3, BAX, and BCL-2 was determined by Western blotting. All assays were performed in triplicate (* P < 0.05, ** P < 0.01, *** P < 0.001) Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-019-1544-y'>31918758</a> https://www.bosterbio.com/products/primary-antibodies/anti-prox1-picoband-trade-antibody-a01985-1-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01985-1-1-s2.0-s2667242125001526-gr5.jpgAnti-PROX1 Antibody Picoband®Double IF staining of Prox1 (red) and MBP (green) with IF (×200) and their intensity profile obtained using ImageJ software, along an ideal straight line (white). Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2667242125001526'>41078399</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01985-1-1-s2.0-s2667242125001526-gr6.jpgAnti-PROX1 Antibody Picoband®(A) IHC images of Prox1 proteins in sciatic nerve tissue of control sciatic nerves and injured sciatic nerves on Day 3, 4, 5, 6, 7 and 14 post-injuries (×200) and the quantification of Prox1 proteins in top right panel (P value was normalized to control group, **** P < 0.0001, n = 5, One-Way ANOVA analysis was used). (B) Western blot and quantitative analysis of Prox1 protein expression in sciatic nerve tissue from control nerves and injured nerves at days 3, 4, 5, 6, 7, and 14 post-injury (**** P < 0.0001, n = 3, One-Way ANOVA analysis was used). Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2667242125001526'>41078399</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01985-1-1-s2.0-s2667242125001526-gr7.jpgAnti-PROX1 Antibody Picoband®MBP protein level was significantly increased by PROX1 overexpression. (A) qPCR quantification of PROX1 overexpression, (B) WB images of MBP and GAPDH protein from cells transfected with NC, pcDNA-vector or pcDNA-prox1, (C) the quantification of (B). (**** P < 0.0001, n = 3, One-Way ANOVA analysis was used). Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2667242125001526'>41078399</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01985-1-prox1-primary-antibodies-wb-testing-1_1.jpgAnti-PROX1 Antibody Picoband®Western blot analysis of PROX1 using anti-PROX1 antibody (A01985-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH7 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX1 antigen affinity purified polyclonal antibody (A01985-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PROX1 at approximately 90 kDa. The expected band size for PROX1 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01985-1-prox1-primary-antibodies-if-testing-1.jpgAnti-PROX1 Antibody Picoband®IF analysis of PROX1 using anti-SGT1/ECD antibody (A01985-1) and anti-Tubulin Alpha antibody (M03989-3). PROX1 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PROX1 Antibody (A01985-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01985-1-prox1-primary-antibodies-ip-testing-1.jpgAnti-PROX1 Antibody Picoband®Immunoprecipitating (IP) PROX1 in SH-SY5Y whole cell lysate. Western blot analysis of PROX1 using anti-PROX1 antibody (A01985-1); Lane 1: SH-SY5Y whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PROX1 antibody in SH-SY5Y whole cell lysate; Lane 3: anti-PROX1 antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PROX1 antigen affinity purified polyclonal antibody (A01985-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PROX1 at approximately 90 kDa. The expected band size for PROX1 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01985-1-prox1-primary-antibodies-fcm-testing-1.jpgAnti-PROX1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-PROX1 antibody (A01985-1). Overlay histogram showing SH-SY5Y cells stained with A01985-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROX1 Antibody (A01985-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-presenilin-1-ps-1-psen1-picoband-trade-antibody-a00138-3-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00138-3-psen1-primary-antibodies-wb-testing-1.jpgAnti-Presenilin 1/PS-1/PSEN1 Antibody Picoband® Western blot analysis of Presenilin 1/PS-1/PSEN1 using anti-Presenilin 1/PS-1/PSEN1 antibody (A00138-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 1/PS-1/PSEN1 antigen affinity purified polyclonal antibody (Catalog # A00138-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Presenilin 1/PS-1/PSEN1 at approximately 53 kDa. The expected band size for Presenilin 1/PS-1/PSEN1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00138-3-psen1-primary-antibodies-if-testing-2.jpgAnti-Presenilin 1/PS-1/PSEN1 Antibody Picoband® IF analysis of Presenilin 1/PS-1/PSEN1 using anti-Presenilin 1/PS-1/PSEN1 antibody (A00138-3). Presenilin 1/PS-1/PSEN1 was detected in an immunocytochemical section of RT4 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Presenilin 1/PS-1/PSEN1 Antibody (A00138-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00138-3-psen1-primary-antibodies-fcm-testing-3.pngAnti-Presenilin 1/PS-1/PSEN1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-Presenilin 1/PS-1/PSEN1 antibody (A00138-3). Overlay histogram showing K562 cells stained with A00138-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Presenilin 1/PS-1/PSEN1 Antibody (A00138-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-psmd8-picoband-trade-antibody-a12465-1-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-wb-testing-1.jpgAnti-PSMD8 Antibody Picoband® Western blot analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD8 antigen affinity purified polyclonal antibody (Catalog # A12465-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD8 at approximately 47 kDa. The expected band size for PSMD8 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-2.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-3.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-4.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-5.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of human lienal pupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-6.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-7.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-8.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-ihc-testing-9.jpgAnti-PSMD8 Antibody Picoband® IHC analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12465-1-psmd8-primary-antibodies-if-testing-10.jpgAnti-PSMD8 Antibody Picoband® IF analysis of PSMD8 using anti-PSMD8 antibody (A12465-1). PSMD8 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMD8 Antibody (A12465-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ptgis-pgis-picoband-trade-antibody-a05053-2-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05053-2-ptgis-primary-antibodies-wb-testing-1.jpgAnti-PTGIS/PGIS Antibody Picoband® Western blot analysis of PTGIS/PGIS using anti-PTGIS/PGIS antibody (A05053-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGIS/PGIS antigen affinity purified polyclonal antibody (Catalog # A05053-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGIS/PGIS at approximately 57 kDa. The expected band size for PTGIS/PGIS is at 57 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05053-2-ptgis-primary-antibodies-fcm-testing-2.pngAnti-PTGIS/PGIS Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-PTGIS/PGIS antibody (A05053-2). Overlay histogram showing U251 cells stained with A05053-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGIS/PGIS Antibody (A05053-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ptpn22-picoband-trade-antibody-a00581-3-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00581-3-ptpn22-primary-antibodies-wb-testing-1.jpgAnti-PTPN22 Antibody Picoband® Western blot analysis of PTPN22 using anti-PTPN22 antibody (A00581-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody (Catalog # A00581-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 92 kDa. The expected band size for PTPN22 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00581-3-ptpn22-primary-antibodies-if-testing-2.jpgAnti-PTPN22 Antibody Picoband® IF analysis of PTPN22 using anti-PTPN22 antibody (A00581-3). PTPN22 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTPN22 Antibody (A00581-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-poliovirus-receptor-pvr-picoband-trade-antibody-a00664-4-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00664-4-pvr-primary-antibodies-wb-testing-1.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (A00664-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HT1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Poliovirus Receptor/PVR antigen affinity purified polyclonal antibody (Catalog # A00664-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00664-4-pvr-primary-antibodies-if-testing-2.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® IF analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (A00664-4). Poliovirus Receptor/PVR was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Poliovirus Receptor/PVR Antibody (A00664-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00664-4-pvr-primary-antibodies-ihc-testing-3.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (A00664-4). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Poliovirus Receptor/PVR Antibody (A00664-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-rcc2-picoband-trade-antibody-a06203-2-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06203-2-rcc2-primary-antibodies-wb-testing-1.jpgAnti-RCC2 Antibody Picoband® Western blot analysis of RCC2 using anti-RCC2 antibody (A06203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCC2 antigen affinity purified polyclonal antibody (Catalog # A06203-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RCC2 at approximately 56 kDa. The expected band size for RCC2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06203-2-rcc2-primary-antibodies-if-testing-2.jpgAnti-RCC2 Antibody Picoband® IF analysis of RCC2 using anti-RCC2 antibody (A06203-2). RCC2 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RCC2 Antibody (A06203-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rhob-picoband-trade-antibody-a01550-3-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01550-3-rhob-primary-antibodies-wb-testing-1.jpgAnti-RHOB Antibody Picoband® Western blot analysis of RHOB using anti-RHOB antibody (A01550-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOB antigen affinity purified polyclonal antibody (Catalog # A01550-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHOB at approximately 22 kDa. The expected band size for RHOB is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rplp0-picoband-trade-antibody-a04349-1-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04349-1-rplp0-primary-antibodies-wb-testing-1.jpgAnti-RPLP0 Antibody Picoband® Western blot analysis of RPLP0 using anti-RPLP0 antibody (A04349-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPLP0 antigen affinity purified polyclonal antibody (Catalog # A04349-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPLP0 at approximately 37 kDa. The expected band size for RPLP0 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04349-1-rplp0-primary-antibodies-if-testing-2.jpgAnti-RPLP0 Antibody Picoband® IF analysis of RPLP0 using anti-RPLP0 antibody (A04349-1). RPLP0 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPLP0 Antibody (A04349-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04349-1-rplp0-primary-antibodies-fcm-testing-3.pngAnti-RPLP0 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RPLP0 antibody (A04349-1). Overlay histogram showing HL-60 cells stained with A04349-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPLP0 Antibody (A04349-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps2-picoband-trade-antibody-a03548-3-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03548-3-rps2-primary-antibodies-wb-testing-1.jpgAnti-RPS2 Antibody Picoband® Western blot analysis of RPS2 using anti-RPS2 antibody (A03548-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS2 antigen affinity purified polyclonal antibody (Catalog # A03548-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS2 at approximately 31 kDa. The expected band size for RPS2 is at 31 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03548-3-rps2-primary-antibodies-ihc-testing-1.jpgAnti-RPS2 Antibody Picoband®IHC analysis of RPS2 using anti-RPS2 antibody (A03548-3). RPS2 was detected in a paraffin-embedded section of human stomch tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS2 Antibody (A03548-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03548-3-rps2-primary-antibodies-if-testing-2.jpgAnti-RPS2 Antibody Picoband® IF analysis of RPS2 using anti-RPS2 antibody (A03548-3). RPS2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS2 Antibody (A03548-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03548-3-rps2-primary-antibodies-fcm-testing-3.pngAnti-RPS2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-RPS2 antibody (A03548-3). Overlay histogram showing U937 cells stained with A03548-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS2 Antibody (A03548-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-msk2-rsk-b-rps6ka4-picoband-trade-antibody-a05545-2-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-wb-testing-1.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® Western blot analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSK2/RSK-B/RPS6KA4 antigen affinity purified polyclonal antibody (Catalog # A05545-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSK2/RSK-B/RPS6KA4 at approximately 86 kDa. The expected band size for MSK2/RSK-B/RPS6KA4 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-ihc-testing-2.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-ihc-testing-3.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-ihc-testing-4.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-ihc-testing-5.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-ihc-testing-6.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-ihc-testing-7.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-ihc-testing-8.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-if-testing-9.jpgAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® IF analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). MSK2/RSK-B/RPS6KA4 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05545-2-rps6ka4-primary-antibodies-fcm-testing-10.pngAnti-MSK2/RSK-B/RPS6KA4 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2). Overlay histogram showing K562 cells stained with A05545-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ryr2-picoband-trade-antibody-a00155-2-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00155-2-ryr2-primary-antibodies-wb-testing-1.jpgAnti-RYR2 Antibody Picoband® Western blot analysis of RYR2 using anti-RYR2 antibody (A00155-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RYR2 antigen affinity purified polyclonal antibody (Catalog # A00155-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RYR2 at approximately 564 kDa. The expected band size for RYR2 is at 564 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00155-2-ryr2-primary-antibodies-ihc-testing-2.jpgAnti-RYR2 Antibody Picoband® IHC analysis of RYR2 using anti-RYR2 antibody (A00155-2). RYR2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RYR2 Antibody (A00155-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00155-2-ryr2-primary-antibodies-ihc-testing-3.jpgAnti-RYR2 Antibody Picoband®IHC analysis of RYR2 using anti-RYR2 antibody (A00155-2). RYR2 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RYR2 Antibody (A00155-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ryr2-picoband-trade-antibody-a00155-3-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00155-3-ryr2-primary-antibodies-wb-testing-1.jpgAnti-RYR2 Antibody Picoband® Western blot analysis of RYR2 using anti-RYR2 antibody (A00155-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RYR2 antigen affinity purified polyclonal antibody (Catalog # A00155-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RYR2 at approximately 564 kDa. The expected band size for RYR2 is at 564 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-skap2-picoband-trade-antibody-a06280-2-boster.html2026-03-17T05:12:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06280-2-skap2-primary-antibodies-wb-testing-1.jpgAnti-SKAP2 Antibody Picoband® Western blot analysis of SKAP2 using anti-SKAP2 antibody (A06280-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKAP2 antigen affinity purified polyclonal antibody (Catalog # A06280-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKAP2 at approximately 41 kDa. The expected band size for SKAP2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06280-2-skap2-primary-antibodies-fcm-testing-2.pngAnti-SKAP2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-SKAP2 antibody (A06280-2). Overlay histogram showing U937 cells stained with A06280-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SKAP2 Antibody (A06280-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-srebp2-srebf2-picoband-trade-antibody-a01678-2-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01678-2-srebf2-primary-antibodies-wb-testing-1.jpgAnti-SREBP2/SREBF2 Antibody Picoband® Western blot analysis of SREBP2/SREBF2 using anti-SREBP2/SREBF2 antibody (A01678-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SREBP2/SREBF2 antigen affinity purified polyclonal antibody (Catalog # A01678-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SREBP2/SREBF2 at approximately 68 kDa. The expected band size for SREBP2/SREBF2 is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01678-2-fsn3-13-e70736-g007.jpgAnti-SREBP2/SREBF2 Antibody Picoband®SIRT1 inhibitor EX‐527 increased M1‐like macrophage and lipid accumulation by EGCG in the model cells. (A) Determination of the optimal concentration of EX‐527. n = 3. (B) Representative WB images and quantitative analysis of the level of iNOS, Arg‐1, SIRT1, IL‐4, P‐STAT6, and P‐AKT1 in the RAW264.7 cells. n = 3 (C) Representative WB images and quantitative analysis of the level of SREBP‐1 and SREBP‐2 in the MPC5 cells. n = 3. (D) Levels of TNF‐α, IL‐18, and IL‐1β in supernatant. n = 6. (E, F) Levels of IL‐4 and IL‐10 in supernatant. n = 6. Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the EGCG group, c p < 0.05. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01678-2-fsn3-13-e70736-g008.jpgAnti-SREBP2/SREBF2 Antibody Picoband®TP improved podocyte lipid accumulation and injury in the aging with DKD model rats. (A) Representative TEM images of podocytes in kidney tissues of rats. 20,000×, n = 3. (B) Representative immunofluorescence colocalization images of SYNPO (green fluorescence) and Adipophilin (red fluorescence). 400×, n = 3. (C) The analysis of mean fluorescence density ratio of Adipophilin to SYNPO assay from each group of rats. (D) Representative WB images and quantification of the expression of Nephrin and Podocin. n = 6. (E) Representative WB images and quantification of the expression of SREBP‐1 and SREBP‐2. n = 6. Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the TP‐L group, c p < 0.05; compared with the TP‐M group, d p < 0.05. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01678-2-fsn3-13-e70736-g011.jpgAnti-SREBP2/SREBF2 Antibody Picoband®SIRT1 agonist SRT1720 increased M2‐like macrophage and decreased lipid deposition by EGCG in the model cells. (A) Determination of the optimal concentration of SRT1720 ( n = 3). (B) Representative WB images and quantitative analysis of the level of iNOS, Arg‐1, SIRT1, IL‐4, P‐STAT6, and P‐AKT1 in the RAW264.7 cells ( n = 3). (C) Representative WB images and quantitative analysis of the level of SREBP‐1 and SREBP‐2 in the MPC5 cells ( n = 3). (D) Levels of TNF‐α, IL‐18, and IL‐1β in supernatant ( n = 6). (E, F) Levels of IL‐4 and IL‐10 in supernatant ( n = 6). Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the EGCG group, c p < 0.05. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01678-2-srebf2-primary-antibodies-fcm-testing-3.pngAnti-SREBP2/SREBF2 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SREBP2/SREBF2 antibody (A01678-2). Overlay histogram showing K562 cells stained with A01678-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SREBP2/SREBF2 Antibody (A01678-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01678-2-srebf2-primary-antibodies-fcm-testing-4.pngAnti-SREBP2/SREBF2 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-SREBP2/SREBF2 antibody (A01678-2). Overlay histogram showing RH35 cells stained with A01678-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SREBP2/SREBF2 Antibody (A01678-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sri-picoband-trade-antibody-a00222-2-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-2-sri-primary-antibodies-wb-testing-1.jpgAnti-SRI Antibody Picoband® Western blot analysis of SRI using anti-SRI antibody (A00222-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRI antigen affinity purified polyclonal antibody (Catalog # A00222-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRI at approximately 22 kDa. The expected band size for SRI is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-2-sri-primary-antibodies-if-testing-2.jpgAnti-SRI Antibody Picoband® IF analysis of SRI using anti-SRI antibody (A00222-2). SRI was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SRI Antibody (A00222-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00222-2-sri-primary-antibodies-fcm-testing-3.pngAnti-SRI Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SRI antibody (A00222-2). Overlay histogram showing 293T cells stained with A00222-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRI Antibody (A00222-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trf1-terf1-picoband-trade-antibody-a02474-2-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02474-2-terf1-primary-antibodies-wb-testing-1.jpgAnti-TRF1/TERF1 Antibody Picoband® Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (A02474-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (Catalog # A02474-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02474-2-terf1-primary-antibodies-if-testing-2.jpgAnti-TRF1/TERF1 Antibody Picoband® IF analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (A02474-2). TRF1/TERF1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRF1/TERF1 Antibody (A02474-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ap2-gamma-tfap2c-picoband-trade-antibody-a01558-2-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01558-2-tfap2c-primary-antibodies-wb-testing-1.jpgAnti-AP2 gamma/TFAP2C Antibody Picoband® Western blot analysis of AP2 Gamma/TFAP2C using anti-AP2 Gamma/TFAP2C antibody (A01558-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2 Gamma/TFAP2C antigen affinity purified polyclonal antibody (Catalog # A01558-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2 Gamma/TFAP2C at approximately 49 kDa. The expected band size for AP2 Gamma/TFAP2C is at 49 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01558-2-tfap2c-primary-antibodies-ihc-testing-2.jpgAnti-AP2 gamma/TFAP2C Antibody Picoband® IHC analysis of AP2 Gamma/TFAP2C using anti-AP2 Gamma/TFAP2C antibody (A01558-2). AP2 Gamma/TFAP2C was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AP2 Gamma/TFAP2C Antibody (A01558-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01558-2-tfap2c-primary-antibodies-ihc-testing-3.jpgAnti-AP2 gamma/TFAP2C Antibody Picoband® IHC analysis of AP2 Gamma/TFAP2C using anti-AP2 Gamma/TFAP2C antibody (A01558-2). AP2 Gamma/TFAP2C was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AP2 Gamma/TFAP2C Antibody (A01558-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01558-2-tfap2c-primary-antibodies-if-testing-4.jpgAnti-AP2 gamma/TFAP2C Antibody Picoband® IF analysis of AP2 Gamma/TFAP2C using anti-AP2 Gamma/TFAP2C antibody (A01558-2). AP2 Gamma/TFAP2C was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AP2 Gamma/TFAP2C Antibody (A01558-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tpi1-picoband-trade-antibody-a02559-2-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02559-2-tpi1-primary-antibodies-wb-testing-1.jpgAnti-TPI1 Antibody Picoband® Western blot analysis of TPI1 using anti-TPI1 antibody (A02559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPI1 antigen affinity purified polyclonal antibody (Catalog # A02559-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPI1 at approximately 27 kDa. The expected band size for TPI1 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02559-2-tpi1-primary-antibodies-fcm-testing-2.pngAnti-TPI1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-TPI1 antibody (A02559-2). Overlay histogram showing K562 cells stained with A02559-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPI1 Antibody (A02559-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trap1-picoband-trade-antibody-a02426-1-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02426-1-trap1-primary-antibodies-wb-testing-1.jpgAnti-TRAP1 Antibody Picoband® Western blot analysis of TRAP1 using anti-TRAP1 antibody (A02426-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAP1 antigen affinity purified polyclonal antibody (Catalog # A02426-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAP1 at approximately 80 kDa. The expected band size for TRAP1 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02426-1-trap1-primary-antibodies-fcm-testing-2.pngAnti-TRAP1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-TRAP1 antibody (A02426-1). Overlay histogram showing HL-60 cells stained with A02426-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAP1 Antibody (A02426-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cip4-trip10-picoband-trade-antibody-a03963-1-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03963-1-trip10-primary-antibodies-wb-testing-1.jpgAnti-Cip4/TRIP10 Antibody Picoband® Western blot analysis of Cip4/TRIP10 using anti-Cip4/TRIP10 antibody (A03963-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cip4/TRIP10 antigen affinity purified polyclonal antibody (Catalog # A03963-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cip4/TRIP10 at approximately 85 kDa. The expected band size for Cip4/TRIP10 is at 68 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03963-1-trip10-primary-antibodies-if-testing-2.jpgAnti-Cip4/TRIP10 Antibody Picoband® IF analysis of Cip4/TRIP10 using anti-Cip4/TRIP10 antibody (A03963-1). Cip4/TRIP10 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cip4/TRIP10 Antibody (A03963-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tubgcp2-picoband-trade-antibody-a10573-1-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10573-1-tubgcp2-primary-antibodies-wb-testing-1.jpgAnti-TUBGCP2 Antibody Picoband® Western blot analysis of TUBGCP2 using anti-TUBGCP2 antibody (A10573-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBGCP2 antigen affinity purified polyclonal antibody (Catalog # A10573-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBGCP2 at approximately 103 kDa. The expected band size for TUBGCP2 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10573-1-tubgcp2-primary-antibodies-if-testing-2.jpgAnti-TUBGCP2 Antibody Picoband® IF analysis of TUBGCP2 using anti-TUBGCP2 antibody (A10573-1). TUBGCP2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TUBGCP2 Antibody (A10573-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tyrosinase-tyr-picoband-trade-antibody-a00326-3-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00326-3-tyr-primary-antibodies-wb-testing-1.jpgAnti-Tyrosinase/TYR Antibody Picoband® Western blot analysis of Tyrosinase/TYR using anti-Tyrosinase/TYR antibody (A00326-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosinase/TYR antigen affinity purified polyclonal antibody (Catalog # A00326-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosinase/TYR at approximately 60-65 kDa. The expected band size for Tyrosinase/TYR is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-usp9x-picoband-trade-antibody-a02594-1-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02594-1-usp9x-primary-antibodies-wb-testing-1.jpgAnti-USP9X Antibody Picoband® Western blot analysis of USP9X using anti-USP9X antibody (A02594-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP9X antigen affinity purified polyclonal antibody (Catalog # A02594-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP9X at approximately 290 kDa. The expected band size for USP9X is at 290 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02594-1-usp9x-primary-antibodies-fcm-testing-2.pngAnti-USP9X Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-USP9X antibody (A02594-1). Overlay histogram showing HEL cells stained with A02594-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP9X Antibody (A02594-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02594-1-usp9x-primary-antibodies-if-testing-3.jpgAnti-USP9X Antibody Picoband® IF analysis of USP9X using anti-USP9X antibody (A02594-1). USP9X was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-USP9X Antibody (A02594-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-vav3-picoband-trade-antibody-a03204-3-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03204-3-vav3-primary-antibodies-wb-testing-1.jpgAnti-VAV3 Antibody Picoband® Western blot analysis of VAV3 using anti-VAV3 antibody (A03204-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAV3 antigen affinity purified polyclonal antibody (Catalog # A03204-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAV3 at approximately 98 kDa. The expected band size for VAV3 is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03204-3-vav3-primary-antibodies-if-testing-2.jpgAnti-VAV3 Antibody Picoband® IF analysis of VAV3 using anti-VAV3 antibody (A03204-3). VAV3 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-VAV3 Antibody (A03204-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-kiaa1429-virma-picoband-trade-antibody-a32283-1-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32283-1-virma-primary-antibodies-wb-testing-1.jpgAnti-KIAA1429/VIRMA Antibody Picoband® Western blot analysis of KIAA1429/VIRMA using anti-KIAA1429/VIRMA antibody (A32283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA1429/VIRMA antigen affinity purified polyclonal antibody (Catalog # A32283-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA1429/VIRMA at approximately 202 kDa. The expected band size for KIAA1429/VIRMA is at 202 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32283-1-virma-primary-antibodies-ihc-testing-2.jpgAnti-KIAA1429/VIRMA Antibody Picoband® IHC analysis of KIAA1429/VIRMA using anti-KIAA1429/VIRMA antibody (A32283-1). KIAA1429/VIRMA was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KIAA1429/VIRMA Antibody (A32283-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32283-1-virma-primary-antibodies-ihc-testing-3.jpgAnti-KIAA1429/VIRMA Antibody Picoband® IHC analysis of KIAA1429/VIRMA using anti-KIAA1429/VIRMA antibody (A32283-1). KIAA1429/VIRMA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KIAA1429/VIRMA Antibody (A32283-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32283-1-virma-primary-antibodies-if-testing-4.jpgAnti-KIAA1429/VIRMA Antibody Picoband® IF analysis of KIAA1429/VIRMA using anti-KIAA1429/VIRMA antibody (A32283-1). KIAA1429/VIRMA was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-KIAA1429/VIRMA Antibody (A32283-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-wnt8b-picoband-trade-antibody-a07933-1-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07933-1-wnt8b-primary-antibodies-wb-testing-1.jpgAnti-WNT8B Antibody Picoband® Western blot analysis of WNT8B using anti-WNT8B antibody (A07933-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT8B antigen affinity purified polyclonal antibody (Catalog # A07933-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT8B at approximately 40-50 kDa. The expected band size for WNT8B is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07933-1-wnt8b-primary-antibodies-if-testing-2.jpgAnti-WNT8B Antibody Picoband® IF analysis of WNT8B using anti-WNT8B antibody (A07933-1). WNT8B was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WNT8B Antibody (A07933-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-ccl14-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1123-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez1123.pngHuman CCL14 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human CCL14 EZ Set ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-bmp-9-picokine-trade-quick-elisa-kit-fek1560-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek1560.pngHuman BMP-9 PicoKine® Quick ELISA KitHuman BMP-9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-endostatin-picokine-trade-quick-elisa-kit-fek1376-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek1376.pngMouse Endostatin PicoKine® Quick ELISA KitMouse Endostatin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/rat-endostatin-picokine-trade-quick-elisa-kit-fek1377-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek1377_1.pngRat Endostatin PicoKine® Quick ELISA KitRat Endostatin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/human-egfr-picokine-trade-quick-elisa-kit-fek0327-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0327.pngHuman EGFR PicoKine® Quick ELISA KitHuman EGFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/picokine-quick-elisa-kits/mouse-egf-picokine-trade-quick-elisa-kit-fek0326-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0326.pngMouse EGF PicoKine® Quick ELISA KitHuman EGFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd7-picokine-elisa-kit-ek2150-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2150.pngHuman CD7 ELISA Kit PicoKine®Human CD7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd58-picokine-elisa-kit-ek2151-boster.html2026-03-10T04:33:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2151.jpgHuman CD58 ELISA Kit PicoKine®Human CD58 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd74-picokine-elisa-kit-ek2152-boster.html2026-03-10T04:33:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2152.jpgHuman CD74 ELISA Kit PicoKine®Human CD74 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd79b-picokine-elisa-kit-ek2153-boster.html2026-03-10T04:33:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2153.pngHuman CD79B ELISA Kit PicoKine®Human CD79B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd300e-picokine-elisa-kit-ek2154-boster.html2026-03-10T04:33:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2154.pngHuman CD300E ELISA Kit PicoKine®Human CD300E PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd300lg-picokine-elisa-kit-ek2155-boster.html2026-03-10T04:33:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2155.jpgHuman CD300LG ELISA Kit PicoKine®Human CD300LG PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd302-picokine-elisa-kit-ek2156-boster.html2026-03-10T04:33:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2156.pngHuman CD302 ELISA Kit PicoKine®Human CD302 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cdo-picokine-elisa-kit-ek2157-boster.html2026-03-10T04:33:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2157.pngHuman CDO ELISA Kit PicoKine®Human CDO PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-adar1-adar-picoband-trade-antibody-a00869-2-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00869-2-adar-primary-antibodies-wb-testing-1.jpgAnti-ADAR1/ADAR Antibody Picoband® Western blot analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR1/ADAR antigen affinity purified polyclonal antibody (Catalog # A00869-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAR1/ADAR at approximately 110 kDa. The expected band size for ADAR1/ADAR is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00869-2-adar-primary-antibodies-ihc-testing-2.jpgAnti-ADAR1/ADAR Antibody Picoband® IHC analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2). ADAR1/ADAR was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAR1/ADAR Antibody (A00869-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00869-2-adar-primary-antibodies-ihc-testing-3.jpgAnti-ADAR1/ADAR Antibody Picoband® IHC analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2). ADAR1/ADAR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAR1/ADAR Antibody (A00869-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00869-2-adar-primary-antibodies-ihc-testing-4.jpgAnti-ADAR1/ADAR Antibody Picoband® IHC analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2). ADAR1/ADAR was detected in a paraffin-embedded section of human renal pelvis squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAR1/ADAR Antibody (A00869-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00869-2-adar-primary-antibodies-ihc-testing-5.jpgAnti-ADAR1/ADAR Antibody Picoband® IHC analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2). ADAR1/ADAR was detected in a paraffin-embedded section of human differentiated adenocarcinoma of the rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAR1/ADAR Antibody (A00869-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00869-2-adar-primary-antibodies-if-testing-6.jpgAnti-ADAR1/ADAR Antibody Picoband® IF analysis of ADAR1/ADAR using anti-ADAR1/ADAR antibody (A00869-2) and Anti-Beta Tubulin Antibody (M01857-3). ADAR1/ADAR was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ADAR1/ADAR Antibody (A00869-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rage-ager-picoband-trade-antibody-a03438-1-boster.html2026-03-17T05:16:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03438-1-ager-primary-antibodies-wb-testing-1.jpgAnti-RAGE/AGER Antibody Picoband® Western blot analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE/AGER antigen affinity purified polyclonal antibody (Catalog # A03438-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE/AGER at approximately 45-58 kDa. The expected band size for RAGE/AGER is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03438-1-ager-primary-antibodies-ihc-testing-3.jpgAnti-RAGE/AGER Antibody Picoband®IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). RAGE/AGER was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAGE/AGER Antibody (A03438-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03438-1-ager-primary-antibodies-ihc-testing-2.jpgAnti-RAGE/AGER Antibody Picoband® IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). RAGE/AGER was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAGE/AGER Antibody (A03438-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03438-1-ager-primary-antibodies-if-testing-3.jpgAnti-RAGE/AGER Antibody Picoband® IF analysis of RAGE/AGER using anti-RAGE/AGER antibody (A03438-1). RAGE/AGER was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAGE/AGER Antibody (A03438-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03438-1-ager-primary-antibodies-fcm-testing-4.pngAnti-RAGE/AGER Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RAGE/AGER antibody (A03438-1). Overlay histogram showing MCF-7 cells stained with A03438-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RAGE/AGER Antibody (A03438-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ankyrin-erythroid-ank-ank1-picoband-trade-antibody-a02716-1-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02716-1-ank1-primary-antibodies-wb-testing-1.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® Western blot analysis of Ankyrin Erythroid/ANK/ANK1 using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ankyrin Erythroid/ANK/ANK1 antigen affinity purified polyclonal antibody (Catalog # A02716-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ankyrin Erythroid/ANK/ANK1 at approximately 250 kDa. The expected band size for Ankyrin Erythroid/ANK/ANK1 is at 206 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02716-1-ank1-primary-antibodies-ihc-testing-2.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® IHC analysis of Ankyrin Erythroid/ANK/ANK1 using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Ankyrin Erythroid/ANK/ANK1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ankyrin Erythroid/ANK/ANK1 Antibody (A02716-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02716-1-ank1-primary-antibodies-ihc-testing-3_1.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® IHC analysis of Ankyrin Erythroid/ANK/ANK1 using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Ankyrin Erythroid/ANK/ANK1 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ankyrin Erythroid/ANK/ANK1 Antibody (A02716-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02716-1-ank1-primary-antibodies-ihc-testing-4_1.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® IHC analysis of Ankyrin Erythroid/ANK/ANK1 using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Ankyrin Erythroid/ANK/ANK1 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ankyrin Erythroid/ANK/ANK1 Antibody (A02716-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02716-1-ank1-primary-antibodies-fcm-testing-6.pngAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Overlay histogram showing HEL cells stained with A02716-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ankyrin Erythroid/ANK/ANK1 Antibody (A02716-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02716-1-ank1-primary-antibodies-if-testing-5.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® IF analysis of Ankyrin Erythroid/ANK/ANK1 using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Ankyrin Erythroid/ANK/ANK1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Ankyrin Erythroid/ANK/ANK1 Antibody (A02716-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ap2m1-picoband-trade-antibody-a06179-3-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06179-3-ap2m1-primary-antibodies-wb-testing-1.jpgAnti-AP2M1 Antibody Picoband® Western blot analysis of AP2M1 using anti-AP2M1 antibody (A06179-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2M1 antigen affinity purified polyclonal antibody (Catalog # A06179-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1 at approximately 50 kDa. The expected band size for AP2M1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06179-3-ap2m1-primary-antibodies-ihc-testing-1.jpgAnti-AP2M1 Antibody Picoband®IHC analysis of AP2M1 using anti-AP2M1 antibody (A06179-3). AP2M1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AP2M1 Antibody (A06179-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06179-3-ap2m1-primary-antibodies-if-testing-2.jpgAnti-AP2M1 Antibody Picoband® IF analysis of AP2M1 using anti-AP2M1 antibody (A06179-3). AP2M1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AP2M1 Antibody (A06179-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06179-3-ap2m1-primary-antibodies-fcm-testing-3.pngAnti-AP2M1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-AP2M1 antibody (A06179-3). Overlay histogram showing MCF-7 cells stained with A06179-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AP2M1 Antibody (A06179-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atg16l1-picoband-trade-antibody-a00526-3-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00526-3-atg16l1-primary-antibodies-wb-testing-1.jpgAnti-ATG16L1 Antibody Picoband® Western blot analysis of ATG16L1 using anti-ATG16L1 antibody (A00526). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG16L1 antigen affinity purified polyclonal antibody (Catalog # A00526) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG16L1 at approximately 68 kDa. The expected band size for ATG16L1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00526-3-atg16l1-primary-antibodies-ihc-testing-2.jpgAnti-ATG16L1 Antibody Picoband® IHC analysis of ATG16L1 using anti-ATG16L1 antibody (A00526-3). ATG16L1 was detected in a paraffin-embedded section of human the renal pelvis is squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG16L1 Antibody (A00526-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00526-3-atg16l1-primary-antibodies-ihc-testing-3.jpgAnti-ATG16L1 Antibody Picoband® IHC analysis of ATG16L1 using anti-ATG16L1 antibody (A00526-3). ATG16L1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG16L1 Antibody (A00526-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00526-3-atg16l1-primary-antibodies-ihc-testing-4.jpgAnti-ATG16L1 Antibody Picoband® IHC analysis of ATG16L1 using anti-ATG16L1 antibody (A00526-3). ATG16L1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG16L1 Antibody (A00526-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00526-3-atg16l1-primary-antibodies-if-testing-5.jpgAnti-ATG16L1 Antibody Picoband® IF analysis of ATG16L1 using anti-ATG16L1 antibody (A00526-3). ATG16L1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG16L1 Antibody (A00526-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-bace1-picoband-trade-antibody-a00322-3-boster.html2026-03-17T05:12:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00322-3-bace1-primary-antibodies-wb-testing-1.jpgAnti-BACE1 Antibody Picoband® Western blot analysis of BACE1 using anti-BACE1 antibody (A00322-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BACE1 antigen affinity purified polyclonal antibody (Catalog # A00322-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BACE1 at approximately 70 kDa. The expected band size for BACE1 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-bard1-picoband-trade-antibody-a01646-2-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01646-2-bard1-primary-antibodies-wb-testing-1.jpgAnti-BARD1 Antibody Picoband® Western blot analysis of BARD1 using anti-BARD1 antibody (A01646-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BARD1 antigen affinity purified polyclonal antibody (Catalog # A01646-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BARD1 at approximately 87 kDa. The expected band size for BARD1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01646-2-bard1-primary-antibodies-fcm-testing-2.pngAnti-BARD1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-BARD1 antibody (A01646-2). Overlay histogram showing HepG2 cells stained with A01646-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BARD1 Antibody (A01646-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bim-bcl2l11-picoband-trade-antibody-a01552-4-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01552-4-bcl2l11-primary-antibodies-wb-testing-1.jpgAnti-Bim/BCL2L11 Antibody Picoband® Western blot analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/BCL2L11 antigen affinity purified polyclonal antibody (Catalog # A01552-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/BCL2L11 at approximately 28 kDa. The expected band size for Bim/BCL2L11 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01552-4-bcl2l11-primary-antibodies-ihc-testing-2.jpgAnti-Bim/BCL2L11 Antibody Picoband® IHC analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Bim/BCL2L11 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bim/BCL2L11 Antibody (A01552-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01552-4-bcl2l11-primary-antibodies-ihc-testing-3.jpgAnti-Bim/BCL2L11 Antibody Picoband® IHC analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Bim/BCL2L11 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bim/BCL2L11 Antibody (A01552-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01552-4-bcl2l11-primary-antibodies-ihc-testing-4.jpgAnti-Bim/BCL2L11 Antibody Picoband® IHC analysis of Bim/BCL2L11 using anti-Bim/BCL2L11 antibody (A01552-4). Bim/BCL2L11 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bim/BCL2L11 Antibody (A01552-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01552-4-bcl2l11-primary-antibodies-fcm-testing-5.pngAnti-Bim/BCL2L11 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-Bim/BCL2L11 antibody (A01552-4). Overlay histogram showing HL-60 cells stained with A01552-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bim/BCL2L11 Antibody (A01552-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-blk-picoband-trade-antibody-a01539-4-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01539-4-blk-primary-antibodies-wb-testing-1.jpgAnti-BLK Antibody Picoband® Western blot analysis of BLK using anti-BLK antibody (A01539-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLK antigen affinity purified polyclonal antibody (Catalog # A01539-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLK at approximately 60 kDa. The expected band size for BLK is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01539-4-blk-primary-antibodies-fcm-testing-2.pngAnti-BLK Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-BLK antibody (A01539-4). Overlay histogram showing Daudi cells stained with A01539-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BLK Antibody (A01539-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-brd3-picoband-trade-antibody-a04044-3-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04044-3-brd3-primary-antibodies-wb-testing-1.jpgAnti-BRD3 Antibody Picoband® Western blot analysis of BRD3 using anti-BRD3 antibody (A04044-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD3 antigen affinity purified polyclonal antibody (Catalog # A04044-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD3 at approximately 80 kDa. The expected band size for BRD3 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04044-3-brd3-primary-antibodies-fcm-testing-2.pngAnti-BRD3 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-BRD3 antibody (A04044-3). Overlay histogram showing 293T cells stained with A04044-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRD3 Antibody (A04044-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-btc-picoband-trade-antibody-a02171-4-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02171-4-btc-primary-antibodies-wb-testing-1.jpgAnti-Btc Antibody Picoband® Western blot analysis of Btc using anti-Btc antibody (A02171-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Btc antigen affinity purified polyclonal antibody (Catalog # A02171-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Btc at approximately 26 kDa. The expected band size for Btc is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02171-4-btc-primary-antibodies-ihc-testing-2.jpgAnti-Btc Antibody Picoband® IHC analysis of Btc using anti-Btc antibody (A02171-4). Btc was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Btc Antibody (A02171-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02171-4-btc-primary-antibodies-ihc-testing-3.jpgAnti-Btc Antibody Picoband® IHC analysis of Btc using anti-Btc antibody (A02171-4). Btc was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Btc Antibody (A02171-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-caspase-10-casp10-picoband-trade-antibody-a02190-2-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02190-2-casp10-primary-antibodies-wb-testing-1.jpgAnti-Caspase-10/CASP10 Antibody Picoband® Western blot analysis of Caspase-10/CASP10 using anti-Caspase-10/CASP10 antibody (A02190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-10/CASP10 antigen affinity purified polyclonal antibody (Catalog # A02190-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-10/CASP10 at approximately 59 kDa. The expected band size for Caspase-10/CASP10 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02190-2-casp10-primary-antibodies-fcm-testing-2.pngAnti-Caspase-10/CASP10 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Caspase-10/CASP10 antibody (A02190-2). Overlay histogram showing SiHa cells stained with A02190-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-10/CASP10 Antibody (A02190-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cbx2-picoband-trade-antibody-a06356-2-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06356-2-cbx2-primary-antibodies-wb-testing-1.jpgAnti-CBX2 Antibody Picoband® Western blot analysis of CBX2 using anti-CBX2 antibody (A06356-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX2 antigen affinity purified polyclonal antibody (Catalog # A06356-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBX2 at approximately 56 kDa. The expected band size for CBX2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06356-2-cbx2-primary-antibodies-ihc-testing-2.jpgAnti-CBX2 Antibody Picoband® IHC analysis of CBX2 using anti-CBX2 antibody (A06356-2). CBX2 was detected in a paraffin-embedded section of human the renal pelvis is squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX2 Antibody (A06356-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06356-2-cbx2-primary-antibodies-ihc-testing-3.jpgAnti-CBX2 Antibody Picoband® IHC analysis of CBX2 using anti-CBX2 antibody (A06356-2). CBX2 was detected in a paraffin-embedded section of human rectum differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX2 Antibody (A06356-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06356-2-cbx2-primary-antibodies-ihc-testing-4.jpgAnti-CBX2 Antibody Picoband® IHC analysis of CBX2 using anti-CBX2 antibody (A06356-2). CBX2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX2 Antibody (A06356-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06356-2-cbx2-primary-antibodies-ihc-testing-5.jpgAnti-CBX2 Antibody Picoband® IHC analysis of CBX2 using anti-CBX2 antibody (A06356-2). CBX2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBX2 Antibody (A06356-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06356-2-cbx2-primary-antibodies-if-testing-6.jpgAnti-CBX2 Antibody Picoband® IF analysis of CBX2 using anti-CBX2 antibody (A06356-2). CBX2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CBX2 Antibody (A06356-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-eotaxin-ccl11-picoband-trade-antibody-a01438-2-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01438-2-ccl11-primary-antibodies-ihc-testing-1.jpgAnti-Eotaxin/Ccl11 Antibody IHC analysis of Eotaxin/Ccl11 using anti-Eotaxin/Ccl11 antibody (A01438-2). Eotaxin/Ccl11 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Eotaxin/Ccl11 Antibody (A01438-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cd36-picoband-trade-antibody-a01189-1-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01189-1-cd36-primary-antibodies-wb-testing-1.jpgAnti-CD36 Antibody Picoband® Western blot analysis of CD36 using anti-CD36 antibody (A01189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD36 antigen affinity purified polyclonal antibody (Catalog # A01189-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01189-1-cd36-primary-antibodies-fcm-testing-2.pngAnti-CD36 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-CD36 antibody (A01189-1). Overlay histogram showing HEL cells stained with A01189-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD36 Antibody (A01189-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-cd99-picoband-trade-antibody-a01724-2-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01724-2-cd99-primary-antibodies-wb-testing-1.jpgAnti-CD99 Antibody Picoband® Western blot analysis of CD99 using anti-CD99 antibody (A01724-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD99 antigen affinity purified polyclonal antibody (Catalog # A01724-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD99 at approximately 28 kDa. The expected band size for CD99 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-chromogranin-a-chga-picoband-trade-antibody-a01256-3-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01256-3-chga-primary-antibodies-wb-testing-1_1.jpgAnti-Chromogranin A/Chga Antibody Picoband® Western blot analysis of Chromogranin A/Chga using anti-Chromogranin A/Chga antibody (A01256-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancrease tissue lysates, Lane 2: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/Chga antigen affinity purified polyclonal antibody (Catalog # A01256-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/Chga at approximately 70-80 kDa. The expected band size for Chromogranin A/Chga is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01256-3-chga-primary-antibodies-if-testing-2.jpgAnti-Chromogranin A/Chga Antibody Picoband® IF analysis of Chromogranin A/Chga using anti-Chromogranin A/Chga antibody (A01256-3). Chromogranin A/Chga was detected in an immunocytochemical section of RM-1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Chromogranin A/Chga Antibody (A01256-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cited1-picoband-trade-antibody-a04351-1-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04351-1-cited1-primary-antibodies-wb-testing-1.jpgAnti-CITED1 Antibody Picoband® Western blot analysis of CITED1 using anti-CITED1 antibody (A04351-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CITED1 antigen affinity purified polyclonal antibody (Catalog # A04351-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CITED1 at approximately 23 kDa. The expected band size for CITED1 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-claudin-2-cldn2-picoband-trade-antibody-a03033-2-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03033-2-cldn2-primary-antibodies-wb-testing-1_1.jpgAnti-Claudin 2/CLDN2 Antibody Picoband® Western blot analysis of Claudin 2/CLDN2 using anti-Claudin 2/CLDN2 antibody (A03033-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 2/CLDN2 antigen affinity purified polyclonal antibody (Catalog # A03033-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 2/CLDN2 at approximately 25 kDa. The expected band size for Claudin 2/CLDN2 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03033-2-cldn2-primary-antibodies-fcm-testing-2.pngAnti-Claudin 2/CLDN2 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Claudin 2/CLDN2 antibody (A03033-2). Overlay histogram showing C6 cells stained with A03033-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Claudin 2/CLDN2 Antibody (A03033-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-calponin-2-cnn2-picoband-trade-antibody-a08680-2-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08680-2-cnn2-primary-antibodies-wb-testing-1.jpgAnti-Calponin 2/CNN2 Antibody Picoband® Western blot analysis of Calponin 2/CNN2 using anti-Calponin 2/CNN2 antibody (A08680-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 2/CNN2 antigen affinity purified polyclonal antibody (Catalog # A08680-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 2/CNN2 at approximately 34 kDa. The expected band size for Calponin 2/CNN2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08680-2-cnn2-primary-antibodies-ihc-testing-2.jpgAnti-Calponin 2/CNN2 Antibody Picoband® IHC analysis of Calponin 2/CNN2 using anti-Calponin 2/CNN2 antibody (A08680-2). Calponin 2/CNN2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calponin 2/CNN2 Antibody (A08680-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08680-2-cnn2-primary-antibodies-fcm-testing-3.pngAnti-Calponin 2/CNN2 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-Calponin 2/CNN2 antibody (A08680-2). Overlay histogram showing Daudi cells stained with A08680-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calponin 2/CNN2 Antibody (A08680-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-calponin-3-cnn3-picoband-trade-antibody-a08267-4-boster.html2026-03-17T05:12:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08267-4-cnn3-primary-antibodies-wb-testing-1.jpgAnti-Calponin 3/CNN3 Antibody Picoband® Western blot analysis of Calponin 3/CNN3 using anti-Calponin 3/CNN3 antibody (A08267-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 3/CNN3 antigen affinity purified polyclonal antibody (Catalog # A08267-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 3/CNN3 at approximately 39 kDa. The expected band size for Calponin 3/CNN3 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08267-4-cnn3-primary-antibodies-fcm-testing-2.pngAnti-Calponin 3/CNN3 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Calponin 3/CNN3 antibody (A08267-4). Overlay histogram showing C6 cells stained with A08267-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calponin 3/CNN3 Antibody (A08267-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-collagen-vi-col6a2-picoband-trade-antibody-a03194-2-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-wb-testing-1.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® Western blot analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen VI/COL6A2 antigen affinity purified polyclonal antibody (Catalog # A03194-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen VI/COL6A2 at approximately 150 kDa. The expected band size for Collagen VI/COL6A2 is at 109 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-ihc-testing-2.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® IHC analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Collagen VI/COL6A2 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen VI/COL6A2 Antibody (A03194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-ihc-testing-3.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® IHC analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Collagen VI/COL6A2 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen VI/COL6A2 Antibody (A03194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-ihc-testing-4.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® IHC analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Collagen VI/COL6A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen VI/COL6A2 Antibody (A03194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-ihc-testing-5.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® IHC analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Collagen VI/COL6A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen VI/COL6A2 Antibody (A03194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-ihc-testing-6.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® IHC analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Collagen VI/COL6A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen VI/COL6A2 Antibody (A03194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-ihc-testing-7.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® IHC analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Collagen VI/COL6A2 was detected in a paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen VI/COL6A2 Antibody (A03194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-2-col6a2-primary-antibodies-ihc-testing-8.jpgAnti-Collagen VI/COL6A2 Antibody Picoband® IHC analysis of Collagen VI/COL6A2 using anti-Collagen VI/COL6A2 antibody (A03194-2). Collagen VI/COL6A2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen VI/COL6A2 Antibody (A03194-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-csde1-nru-picoband-trade-antibody-a05114-1-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05114-1-csde1-primary-antibodies-wb-testing-1.jpgAnti-CSDE1/NRU Antibody Picoband® Western blot analysis of CSDE1/NRU using anti-CSDE1/NRU antibody (A05114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSDE1/NRU antigen affinity purified polyclonal antibody (Catalog # A05114-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSDE1/NRU at approximately 89 kDa. The expected band size for CSDE1/NRU is at 89 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05114-1-csde1-primary-antibodies-fcm-testing-2.pngAnti-CSDE1/NRU Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-CSDE1/NRU antibody (A05114-1). Overlay histogram showing THP-1 cells stained with A05114-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSDE1/NRU Antibody (A05114-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-csnk2a2-picoband-trade-antibody-a04722-1-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-wb-testing-1.jpgAnti-CSNK2A2 Antibody Picoband® Western blot analysis of CSNK2A2 using anti-CSNK2A2 antibody (A04722-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2A2 antigen affinity purified polyclonal antibody (Catalog # A04722-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK2A2 at approximately 39 kDa. The expected band size for CSNK2A2 is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-2_1.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-3_1.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-4.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-5.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-6.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-7.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-8.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-fcm-testing-13.pngAnti-CSNK2A2 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-CSNK2A2 antibody (A04722-1). Overlay histogram showing Caco-2 cells stained with A04722-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSNK2A2 Antibody (A04722-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-9.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-fcm-testing-14.pngAnti-CSNK2A2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-CSNK2A2 antibody (A04722-1). Overlay histogram showing THP-1 cells stained with A04722-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSNK2A2 Antibody (A04722-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-10.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-ihc-testing-11_1.jpgAnti-CSNK2A2 Antibody Picoband® IHC analysis of CSNK2K2 using anti-CSNK2K2 antibody (A04722-1). CSNK2K2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2K2 Antibody (A04722-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04722-1-csnk2k2-primary-antibodies-if-testing-12.jpgAnti-CSNK2A2 Antibody Picoband® IF analysis of CSNK2A2 using anti-CSNK2A2 antibody (A04722-1). CSNK2A2 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CSNK2A2 Antibody (A04722-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-csnk2b-picoband-trade-antibody-a03475-1-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03475-1-csnk2b-primary-antibodies-wb-testing-1.jpgAnti-CSNK2B Antibody Picoband® Western blot analysis of CSNK2B using anti-CSNK2B antibody (A03475-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2B antigen affinity purified polyclonal antibody (Catalog # A03475-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK2B at approximately 25 kDa. The expected band size for CSNK2B is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-6.jpgAnti-CSNK2B Antibody Picoband®IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1). CSNK2B was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-2.jpgAnti-CSNK2B Antibody Picoband® IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1). CSNK2B was detected in a paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-3.jpgAnti-CSNK2B Antibody Picoband® IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1). CSNK2B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-4.jpgAnti-CSNK2B Antibody Picoband® IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1). CSNK2B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-5.jpgAnti-CSNK2B Antibody Picoband® IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1). CSNK2B was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cyp11a1-picoband-trade-antibody-a01071-2-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01071-2-cyp11a1-primary-antibodies-wb-testing-1.jpgAnti-CYP11A1 Antibody Picoband® Western blot analysis of CYP11A1 using anti-CYP11A1 antibody (A01071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP11A1 antigen affinity purified polyclonal antibody (Catalog # A01071-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP11A1 at approximately 50 kDa. The expected band size for CYP11A1 is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cyp26a1-picoband-trade-antibody-a03646-3-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03646-3-cyp26a1-primary-antibodies-wb-testing-1.jpgAnti-CYP26A1 Antibody Picoband® Western blot analysis of CYP26A1 using anti-CYP26A1 antibody (A03646-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat H9C2(2-1) whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP26A1 antigen affinity purified polyclonal antibody (Catalog # A03646-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP26A1 at approximately 56 kDa. The expected band size for CYP26A1 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-dcp1a-picoband-trade-antibody-a04587-2-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04587-2-dcp1a-primary-antibodies-wb-testing-1.jpgAnti-DCP1A Antibody Picoband® Western blot analysis of DCP1A using anti-DCP1A antibody (A04587-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCP1A antigen affinity purified polyclonal antibody (Catalog # A04587-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DCP1A at approximately 75 kDa. The expected band size for DCP1A is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04587-2-dcp1a-primary-antibodies-if-testing-2.jpgAnti-DCP1A Antibody Picoband® IF analysis of DCP1A using anti-DCP1A antibody (A04587-2). DCP1A was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DCP1A Antibody (A04587-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04587-2-dcp1a-primary-antibodies-fcm-testing-3.pngAnti-DCP1A Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-DCP1A antibody (A04587-2). Overlay histogram showing Daudi cells stained with A04587-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DCP1A Antibody (A04587-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-xtp3tpa-dctpp1-picoband-trade-antibody-a11334-1-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11334-1-dctpp1-primary-antibodies-wb-testing-1.jpgAnti-XTP3TPA/DCTPP1 Antibody Picoband® Western blot analysis of XTP3TPA/DCTPP1 using anti-XTP3TPA/DCTPP1 antibody (A11334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XTP3TPA/DCTPP1 antigen affinity purified polyclonal antibody (Catalog # A11334-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XTP3TPA/DCTPP1 at approximately 19 kDa. The expected band size for XTP3TPA/DCTPP1 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11334-1-dctpp1-primary-antibodies-fcm-testing-2.pngAnti-XTP3TPA/DCTPP1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-XTP3TPA/DCTPP1 antibody (A11334-1). Overlay histogram showing JK cells stained with A11334-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XTP3TPA/DCTPP1 Antibody (A11334-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11334-1-dctpp1-primary-antibodies-ihc-testing-3.jpgAnti-XTP3TPA/DCTPP1 Antibody Picoband® IHC analysis of XTP3TPA/DCTPP1 using anti-XTP3TPA/DCTPP1 antibody (A11334-1). XTP3TPA/DCTPP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XTP3TPA/DCTPP1 Antibody (A11334-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11334-1-dctpp1-primary-antibodies-ihc-testing-4.jpgAnti-XTP3TPA/DCTPP1 Antibody Picoband® IHC analysis of XTP3TPA/DCTPP1 using anti-XTP3TPA/DCTPP1 antibody (A11334-1). XTP3TPA/DCTPP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XTP3TPA/DCTPP1 Antibody (A11334-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11334-1-dctpp1-primary-antibodies-ihc-testing-5.jpgAnti-XTP3TPA/DCTPP1 Antibody Picoband® IHC analysis of XTP3TPA/DCTPP1 using anti-XTP3TPA/DCTPP1 antibody (A11334-1). XTP3TPA/DCTPP1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XTP3TPA/DCTPP1 Antibody (A11334-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ddx3-ddx3x-picoband-trade-antibody-a00751-6-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00751-6-ddx3x-primary-antibodies-wb-testing-1.jpgAnti-DDX3/DDX3X Antibody Picoband® Western blot analysis of DDX3/DDX3X using anti-DDX3/DDX3X antibody (A00751-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat C6 whole cell lyates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX3/DDX3X antigen affinity purified polyclonal antibody (Catalog # A00751-6) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX3/DDX3X at approximately 73 kDa. The expected band size for DDX3/DDX3X is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00751-6-ddx3x-primary-antibodies-if-testing-2.jpgAnti-DDX3/DDX3X Antibody Picoband® IF analysis of DDX3/DDX3X using anti-DDX3/DDX3X antibody (A00751-6). DDX3/DDX3X was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX3/DDX3X Antibody (A00751-6) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00751-6-ddx3x-primary-antibodies-fcm-testing-3.pngAnti-DDX3/DDX3X Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-ADAR1/ADAR antibody (A00751-6). Overlay histogram showing A549 cells stained with A00751-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAR1/ADAR Antibody (A00751-6, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00751-6-ddx3x-primary-antibodies-fcm-testing-4.pngAnti-DDX3/DDX3X Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-ADAR1/ADAR antibody (A00751-6). Overlay histogram showing ANA-1 cells stained with A00751-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAR1/ADAR Antibody (A00751-6, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00751-6-ddx3x-primary-antibodies-fcm-testing-5.pngAnti-DDX3/DDX3X Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-ADAR1/ADAR antibody (A00751-6). Overlay histogram showing C6 cells stained with A00751-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAR1/ADAR Antibody (A00751-6, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dlk-1-dlk1-picoband-trade-antibody-a00513-5-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00513-5-dlk1-primary-antibodies-wb-testing-1_1.jpgAnti-DLK-1/DLK1 Antibody Picoband®Western blot analysis of DLK-1/DLK1 using anti-DLK-1/DLK1 antibody (A00513-5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLK-1/DLK1 antigen affinity purified polyclonal antibody (Catalog # A00513-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLK-1/DLK1 at approximately 41 kDa. The expected band size for DLK-1/DLK1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00513-5-dlk1-primary-antibodies-ihc-testing-1.jpgAnti-DLK-1/DLK1 Antibody Picoband®IHC analysis of DLK-1/DLK1 using anti-DLK-1/DLK1 antibody (A00513-5). DLK-1/DLK1 was detected in a paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLK-1/DLK1 Antibody (A00513-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00513-5-dlk1-primary-antibodies-fcm-testing-1.jpgAnti-DLK-1/DLK1 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-DLK-1/DLK1 antibody (A00513-5). Overlay histogram showing A549 cells stained with A00513-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DLK-1/DLK1 Antibody (A00513-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dlx2-picoband-trade-antibody-a03617-2-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03617-2-dlx2-primary-antibodies-wb-testing-1.jpgAnti-DLX2 Antibody Picoband® Western blot analysis of DLX2 using anti-DLX2 antibody (A03617-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLX2 antigen affinity purified polyclonal antibody (Catalog # A03617-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLX2 at approximately 37 kDa. The expected band size for DLX2 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03617-2-dlx2-primary-antibodies-ihc-testing-5.jpgAnti-DLX2 Antibody Picoband®IHC analysis of DLX2 using anti-DLX2 antibody (A03617-2). DLX2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLX2 Antibody (A03617-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03617-2-dlx2-primary-antibodies-ihc-testing-2.jpgAnti-DLX2 Antibody Picoband® IHC analysis of DLX2 using anti-DLX2 antibody (A03617-2). DLX2 was detected in a paraffin-embedded section of differentiated adenocarcinoma of the human rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLX2 Antibody (A03617-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03617-2-dlx2-primary-antibodies-ihc-testing-3.jpgAnti-DLX2 Antibody Picoband® IHC analysis of DLX2 using anti-DLX2 antibody (A03617-2). DLX2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLX2 Antibody (A03617-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03617-2-dlx2-primary-antibodies-ihc-testing-4.jpgAnti-DLX2 Antibody Picoband® IHC analysis of DLX2 using anti-DLX2 antibody (A03617-2). DLX2 was detected in a paraffin-embedded section of squamous metaplasia of human renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLX2 Antibody (A03617-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03617-2-dlx2-primary-antibodies-fcm-testing-5.pngAnti-DLX2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-DLX2 antibody (A03617-2). Overlay histogram showing 293T cells stained with A03617-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DLX2 Antibody (A03617-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hsp40-dnajb1-picoband-trade-antibody-a03100-1-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03100-1-dnajb1-primary-antibodies-wb-testing-1.jpgAnti-Hsp40/DNAJB1 Antibody Picoband® Western blot analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp40/DNAJB1 antigen affinity purified polyclonal antibody (Catalog # A03100-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp40/DNAJB1 at approximately 38 kDa. The expected band size for Hsp40/DNAJB1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03100-1-dnajb1-primary-antibodies-ihc-testing-2.jpgAnti-Hsp40/DNAJB1 Antibody Picoband® IHC analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-1). Hsp40/DNAJB1 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsp40/DNAJB1 Antibody (A03100-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03100-1-dnajb1-primary-antibodies-if-testing-3.jpgAnti-Hsp40/DNAJB1 Antibody Picoband® IF analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-1). Hsp40/DNAJB1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Hsp40/DNAJB1 Antibody (A03100-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03100-1-dnajb1-primary-antibodies-fcm-testing-4.pngAnti-Hsp40/DNAJB1 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Hsp40/DNAJB1 antibody (A03100-1). Overlay histogram showing C6 cells stained with A03100-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp40/DNAJB1 Antibody (A03100-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dph5-picoband-trade-antibody-a11989-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11989-1-dph5-primary-antibodies-wb-testing-1.jpgAnti-DPH5 Antibody Picoband® Western blot analysis of DPH5 using anti-DPH5 antibody (A11989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPH5 antigen affinity purified polyclonal antibody (Catalog # A11989-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DPH5 at approximately 37 kDa. The expected band size for DPH5 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11989-1-dph5-primary-antibodies-if-testing-2.jpgAnti-DPH5 Antibody Picoband® IF analysis of DPH5 using anti-DPH5 antibody (A11989-1). DPH5 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DPH5 Antibody (A11989-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11989-1-dph5-primary-antibodies-fcm-testing-3.pngAnti-DPH5 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-DPH5 antibody (A11989-1). Overlay histogram showing A549 cells stained with A11989-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPH5 Antibody (A11989-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dpy19l1-picoband-trade-antibody-a16659-1-boster.html2026-03-17T05:12:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16659-1-dpy19l1-primary-antibodies-wb-testing-1.jpgAnti-DPY19L1 Antibody Picoband® Western blot analysis of DPY19L1 using anti-DPY19L1 antibody (A16659-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPY19L1 antigen affinity purified polyclonal antibody (Catalog # A16659-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DPY19L1 at approximately 77 kDa. The expected band size for DPY19L1 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16659-1-dpy19l1-primary-antibodies-ihc-testing-2.jpgAnti-DPY19L1 Antibody Picoband® IHC analysis of DPY19L1 using anti-DPY19L1 antibody (A16659-1). DPY19L1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DPY19L1 Antibody (A16659-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16659-1-dpy19l1-primary-antibodies-ihc-testing-3.jpgAnti-DPY19L1 Antibody Picoband® IHC analysis of DPY19L1 using anti-DPY19L1 antibody (A16659-1). DPY19L1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DPY19L1 Antibody (A16659-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16659-1-dpy19l1-primary-antibodies-fcm-testing-4.pngAnti-DPY19L1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-DPY19L1 antibody (A16659-1). Overlay histogram showing HEL cells stained with A16659-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPY19L1 Antibody (A16659-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tceb2-elongin-b-elob-picoband-trade-antibody-a31718-2-boster.html2026-03-17T05:12:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31718-2-elob-primary-antibodies-wb-testing-1.jpgAnti-TCEB2/Elongin-B/ELOB Antibody Picoband® Western blot analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (A31718-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCEB2/Elongin-B/ELOB antigen affinity purified polyclonal antibody (Catalog # A31718-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCEB2/Elongin-B/ELOB at approximately 17 kDa. The expected band size for TCEB2/Elongin-B/ELOB is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31718-2-elob-primary-antibodies-ihc-testing-5.jpgAnti-TCEB2/Elongin-B/ELOB Antibody Picoband®IHC analysis of TCEB2/ELOB using anti-TCEB2/ELOB antibody (A31718-2). TCEB2/ELOB was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCEB2/ELOB Antibody (A31718-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31718-2-elob-primary-antibodies-ihc-testing-2.jpgAnti-TCEB2/Elongin-B/ELOB Antibody Picoband® IHC analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (A31718-2). TCEB2/Elongin-B/ELOB was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCEB2/Elongin-B/ELOB Antibody (A31718-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31718-2-elob-primary-antibodies-ihc-testing-3.jpgAnti-TCEB2/Elongin-B/ELOB Antibody Picoband® IHC analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (A31718-2). TCEB2/Elongin-B/ELOB was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCEB2/Elongin-B/ELOB Antibody (A31718-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31718-2-elob-primary-antibodies-ihc-testing-4.jpgAnti-TCEB2/Elongin-B/ELOB Antibody Picoband® IHC analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (A31718-2). TCEB2/Elongin-B/ELOB was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCEB2/Elongin-B/ELOB Antibody (A31718-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31718-2-elob-primary-antibodies-if-testing-5.jpgAnti-TCEB2/Elongin-B/ELOB Antibody Picoband® IF analysis of TCEB2/Elongin-B/ELOB using anti-TCEB2/Elongin-B/ELOB antibody (A31718-2). TCEB2/Elongin-B/ELOB was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TCEB2/Elongin-B/ELOB Antibody (A31718-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31718-2-elob-primary-antibodies-fcm-testing-6.pngAnti-TCEB2/Elongin-B/ELOB Antibody Picoband® Flow Cytometry analysis of JK cells using anti-TCEB2/Elongin-B/ELOB antibody (A31718-2). Overlay histogram showing JK cells stained with A31718-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TCEB2/Elongin-B/ELOB Antibody (A31718-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-elongin-c-eloc-picoband-trade-antibody-a31720-1-boster.html2026-03-17T05:12:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-wb-testing-1.jpgAnti-Elongin-C/ELOC Antibody Picoband® Western blot analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Elongin-C/ELOC antigen affinity purified polyclonal antibody (Catalog # A31720-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Elongin-C/ELOC at approximately 14 kDa. The expected band size for Elongin-C/ELOC is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-ihc-testing-2.jpgAnti-Elongin-C/ELOC Antibody Picoband® IHC analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-ihc-testing-3.jpgAnti-Elongin-C/ELOC Antibody Picoband® IHC analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-ihc-testing-4.jpgAnti-Elongin-C/ELOC Antibody Picoband® IHC analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in a paraffin-embedded section of human squamous metaplasia of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-ihc-testing-5.jpgAnti-Elongin-C/ELOC Antibody Picoband® IHC analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-ihc-testing-6.jpgAnti-Elongin-C/ELOC Antibody Picoband® IHC analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-ihc-testing-7.jpgAnti-Elongin-C/ELOC Antibody Picoband® IHC analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-ihc-testing-8.jpgAnti-Elongin-C/ELOC Antibody Picoband® IHC analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-if-testing-9.jpgAnti-Elongin-C/ELOC Antibody Picoband® IF analysis of Elongin-C/ELOC using anti-Elongin-C/ELOC antibody (A31720-1). Elongin-C/ELOC was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Elongin-C/ELOC Antibody (A31720-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-fcm-testing-10.pngAnti-Elongin-C/ELOC Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-Elongin-C/ELOC antibody (A31720-1). Overlay histogram showing A549 cells stained with A31720-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Elongin-C/ELOC Antibody (A31720-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-fcm-testing-11.pngAnti-Elongin-C/ELOC Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Elongin-C/ELOC antibody (A31720-1). Overlay histogram showing ANA-1 cells stained with A31720-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Elongin-C/ELOC Antibody (A31720-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31720-1-eloc-primary-antibodies-fcm-testing-12.pngAnti-Elongin-C/ELOC Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-Elongin-C/ELOC antibody (A31720-1). Overlay histogram showing RH35 cells stained with A31720-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Elongin-C/ELOC Antibody (A31720-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eno3-picoband-trade-antibody-a06845-5-boster.html2026-03-17T05:12:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06845-5-eno3-primary-antibodies-wb-testing-1.jpgAnti-ENO3 Antibody Picoband® Western blot analysis of ENO3 using anti-ENO3 antibody (A06845-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO3 antigen affinity purified polyclonal antibody (Catalog # A06845-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO3 at approximately 50 kDa. The expected band size for ENO3 is at 50 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06845-5-eno3-primary-antibodies-ihc-testing-2.jpgAnti-ENO3 Antibody Picoband® IHC analysis of ENO3 using anti-ENO3 antibody (A06845-5). ENO3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO3 Antibody (A06845-5) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-kat3b-p300-ep300-picoband-trade-antibody-a00117-boster.html2026-03-17T05:12:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00117-ep300-primary-antibodies-wb-testing-1.jpgAnti-KAT3B/p300/EP300 Antibody Picoband® Western blot analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/P300/EP300 antigen affinity purified polyclonal antibody (Catalog # A00117) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/P300/EP300 at approximately 264-300 kDa. The expected band size for KAT3B/P300/EP300 is at 264 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00117-ep300-primary-antibodies-ihc-testing-2.jpgAnti-KAT3B/p300/EP300 Antibody Picoband® IHC analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). KAT3B/P300/EP300 was detected in a paraffin-embedded section of differentiated adenocarcinoma of the human rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KAT3B/P300/EP300 Antibody (A00117) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00117-ep300-primary-antibodies-ihc-testing-3.jpgAnti-KAT3B/p300/EP300 Antibody Picoband® IHC analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). KAT3B/P300/EP300 was detected in a paraffin-embedded section of human classic Hodgkin's lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KAT3B/P300/EP300 Antibody (A00117) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00117-ep300-primary-antibodies-ihc-testing-4.jpgAnti-KAT3B/p300/EP300 Antibody Picoband® IHC analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). KAT3B/P300/EP300 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KAT3B/P300/EP300 Antibody (A00117) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00117-ep300-primary-antibodies-ihc-testing-5.jpgAnti-KAT3B/p300/EP300 Antibody Picoband® IHC analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). KAT3B/P300/EP300 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KAT3B/P300/EP300 Antibody (A00117) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00117-ep300-primary-antibodies-if-testing-6.jpgAnti-KAT3B/p300/EP300 Antibody Picoband® IF analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). KAT3B/P300/EP300 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-KAT3B/P300/EP300 Antibody (A00117) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00117-ep300-primary-antibodies-fcm-testing-7_1.pngAnti-KAT3B/p300/EP300 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-KAT3B/P300/EP300 antibody (A00117). Overlay histogram showing 293T cells stained with A00117 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KAT3B/P300/EP300 Antibody (A00117, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ezrin-ezr-picoband-trade-antibody-a01750-2-boster.html2026-03-17T05:12:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01750-2-ezr-primary-antibodies-wb-testing-1.jpgAnti-Ezrin/EZR Antibody Picoband® Western blot analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ezrin/EZR antigen affinity purified polyclonal antibody (Catalog # A01750-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ezrin/EZR at approximately 81 kDa. The expected band size for Ezrin/EZR is at 69 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01750-2-ezr-primary-antibodies-ihc-testing-2.jpgAnti-Ezrin/EZR Antibody Picoband® IHC analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Ezrin/EZR was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ezrin/EZR Antibody (A01750-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01750-2-ezr-primary-antibodies-ihc-testing-3.jpgAnti-Ezrin/EZR Antibody Picoband® IHC analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Ezrin/EZR was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ezrin/EZR Antibody (A01750-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01750-2-ezr-primary-antibodies-ihc-testing-4.jpgAnti-Ezrin/EZR Antibody Picoband® IHC analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Ezrin/EZR was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ezrin/EZR Antibody (A01750-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01750-2-ezr-primary-antibodies-ihc-testing-5.jpgAnti-Ezrin/EZR Antibody Picoband® IHC analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Ezrin/EZR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ezrin/EZR Antibody (A01750-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01750-2-ezr-primary-antibodies-if-testing-6.jpgAnti-Ezrin/EZR Antibody Picoband® IF analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Ezrin/EZR was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Ezrin/EZR Antibody (A01750-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01750-2-ezr-primary-antibodies-fcm-testing-7.pngAnti-Ezrin/EZR Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Ezrin/EZR antibody (A01750-2). Overlay histogram showing THP-1 cells stained with A01750-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ezrin/EZR Antibody (A01750-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fkbp52-fkbp4-picoband-trade-antibody-a02165-3-boster.html2026-03-17T05:12:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02165-3-fkbp4-primary-antibodies-wb-testing-1.jpgAnti-FKBP52/FKBP4 Antibody Picoband® Western blot analysis of FKBP52/FKBP4 using anti-FKBP52/FKBP4 antibody (A02165-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP52/FKBP4 antigen affinity purified polyclonal antibody (Catalog # A02165-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP52/FKBP4 at approximately 56-59 kDa. The expected band size for FKBP52/FKBP4 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-foxg1-picoband-trade-antibody-a01852-2-boster.html2026-03-17T05:12:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01852-2-foxg1-primary-antibodies-wb-testing-1.jpgAnti-FOXG1 Antibody Picoband® Western blot analysis of FOXG1 using anti-FOXG1 antibody (A01852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXG1 antigen affinity purified polyclonal antibody (Catalog # A01852-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXG1 at approximately 52 kDa. The expected band size for FOXG1 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-foxred2-picoband-trade-antibody-a12703-1-boster.html2026-03-17T05:12:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12703-1-foxred2-primary-antibodies-wb-testing-1.jpgAnti-FOXRED2 Antibody Picoband® Western blot analysis of FOXRED2 using anti-FOXRED2 antibody (A12703-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXRED2 antigen affinity purified polyclonal antibody (Catalog # A12703-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXRED2 at approximately 80 kDa. The expected band size for FOXRED2 is at 80 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ggh-picoband-trade-antibody-a03161-2-boster.html2026-03-17T05:12:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03161-2-ggh-primary-antibodies-wb-testing-1.jpgAnti-GGH Antibody Picoband® Western blot analysis of GGH using anti-GGH antibody (A03161-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGH antigen affinity purified polyclonal antibody (Catalog # A03161-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGH at approximately 36 kDa. The expected band size for GGH is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-glutaredoxin-1-glrx-picoband-trade-antibody-a03392-1-boster.html2026-03-17T05:12:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03392-1-glrx-primary-antibodies-wb-testing-1.jpgAnti-Glutaredoxin 1/GLRX Antibody Picoband® Western blot analysis of Glutaredoxin 1/GLRX using anti-Glutaredoxin 1/GLRX antibody (A03392-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutaredoxin 1/GLRX antigen affinity purified polyclonal antibody (Catalog # A03392-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutaredoxin 1/GLRX at approximately 12 kDa. The expected band size for Glutaredoxin 1/GLRX is at 12 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03392-1-glrx-primary-antibodies-ihc-testing-1.jpgAnti-Glutaredoxin 1/GLRX Antibody Picoband®IHC analysis of GLRX using anti-GLRX antibody (A03392-1). GLRX was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLRX Antibody (A03392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03392-1-glrx-primary-antibodies-if-testing-2.jpgAnti-Glutaredoxin 1/GLRX Antibody Picoband® IF analysis of Glutaredoxin 1/GLRX using anti-Glutaredoxin 1/GLRX antibody (A03392-1). Glutaredoxin 1/GLRX was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Glutaredoxin 1/GLRX Antibody (A03392-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03392-1-glrx-primary-antibodies-fcm-testing-3.pngAnti-Glutaredoxin 1/GLRX Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-Glutaredoxin 1/GLRX antibody (A03392-1). Overlay histogram showing Hela cells stained with A03392-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutaredoxin 1/GLRX Antibody (A03392-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-glutathione-peroxidase-4-gpx4-picoband-trade-antibody-a02059-1-boster.html2026-03-17T05:12:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-gpx4-primary-antibodies-wb-testing-1_1.jpgAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (Catalog # A02059-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 19 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-13578_2021_734_fig1_html.pngAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®Ferroptosis is present in CLP-induced AKI. Mice were subjected to CLP, then the kidneys were collected at the indicated time. A , B Shown are representative western blotting and quantification of GPX4 and PTGS2. C–E Quantitative analyses of MDA, GSH, and non-heme iron. F Quantitative analyses of Perl’s stain. G Representative Perl’s stain images. H Representative transmission electron microscopy (TEM) images. The black arrow indicates ferroptosis-like mitochondria. I Quantitative analyses of mitochondrial length. Mice were pretreated with liproxstatin-1 (10 mg/kg, ip) or DFO (20 mg/kg, ip) 0.5 h before being subjected to CLP. All kidney samples were collected at 24 h after CLP. J–K Shown are representative Hematoxylin-eosin (HE) stains and Periodic Acid-Schiff (PAS) stain. L Histological analyses of renal tubular injury. M Quantitative analyses of IHC stain of NGAL. N Representative immunohistochemistry (IHC) images for NGAL. O , P Quantitative analyses of Scr and BUN. n = 6 mice/group, mean ± SD were presented. *P < 0.05, **P < 0.01 and ns : P > 0.05 . ip: intraperitoneal Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-13578_2021_734_fig2_html.pngAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®Effects of MCTR1 on ferroptosis in CLP-induced AKI. Mice were given MCTR1 once, twice, or not during the 24 h experiment. The once-daily administration mode (qd): Mice were given MCTR1 (200 ng/mice, iv) 0.5 h before being subjected to CLP. The twice-daily administration mode (bid): Mice were given MCTR1 (200 ng/mice, iv) 0.5 h before being subjected to CLP, and then an additional MCTR1 (200 ng/mice, iv) was given 12 h after CLP. All kidney samples were collected at 24 h after CLP. A , B Shown are representative western blotting and quantification of GPX4 and PTGS2. C–E Quantitative analyses of MDA, GSH, and non-heme iron. F Quantitative analyses of Perl’s stain. G Representative Perl’s stain images. H Representative TEM images. The black arrow indicates ferroptosis-like mitochondria. I Quantitative analyses of mitochondrial length. J Histological analyses of renal tubular injury. K , L Shown are representative HE stains and PAS stain. M Representative IHC images for NGAL. N Quantitative analyses of IHC stain of NGAL. O , P Quantitative analyses of serum Scr and BUN. n = 6 mice/group, mean ± SD were presented. **P < 0.01 . iv: intravenous Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-13578_2021_734_fig3_html.pngAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®Effects of Nrf2 on MCTR1-regulated ferroptosis in CLP-induced AKI. Mice were given ML385 (30 mg/kg, ip, qd) for 7 d. On day 7, ML385 injection with or without a twice-daily administration mode of MCTR1 as described before in the following experiment. All kidney samples were collected at 24 h after CLP. A–D Shown are representative western blotting and quantification of Nrf2, GPX4, and PTGS2. E – G Quantitative analyses of MDA, GSH, and non-heme iron. H Quantitative analyses of Perl’s stain. I Representative Perl’s stain images. J Representative TEM images. The black arrow indicates ferroptosis-like mitochondria. K Quantitative analyses of mitochondrial length. L Histological analyses of renal tubular injury. M , N Representative HE stains and PAS stain. O Representative IHC images for NGAL. P Quantitative analyses of IHC stain of NGAL. Q , R Quantitative analyses of serum Scr and BUN. n = 6 mice/group, mean ± SD were presented. **P < 0.01 Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-13578_2021_734_fig4_html.pngAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®Effects of MCTR1 on LPS-induced lipid peroxidation and ferroptosis in vitro. A Viability curves for HK-2 cells treated with different concentrations (0, 1, 3, 10, 30, 100, and 300 nM) of MCTR1 and LPS (1 ug/ml) for 8 h. B Viability curves for HK-2 cells treated with different concentrations (0, 1, 3, 10, 30, 100, and 300 nM) of MCTR1 for 0 and 8 h. C HK-2 cells treated with MCTR1 (100 nM) and LPS (1 ug/ml) for 8 h, and the visualization of cell viability were evaluated by phase-contrast microscopy. D , E Representative western blotting and quantification of GPX4 and PTGS2 protein. F – H Quantitative analyses of MDA, GSH and, non-heme iron. I Quantitative analyses of oxidized C11-BODIPY 581/591 probe by flow cytometry. J Representative images of C11-BODIPY 581/591 fluorescent ratio-probe. K Representative TEM images. The black arrow indicates ferroptosis-like mitochondria. L Quantitative analyses of mitochondrial length. n = 3, mean ± SD were presented. **P < 0.01 Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-13578_2021_734_fig5_html.pngAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®Nrf2 is involved in the inhibitory effects of MCTR1 on LPS-induced ferroptosis. A , B HK-2 cells were treated with MCTR1 (100 nM) and LPS (1 ug/ml) for 8 h. Shown are representative western blotting and quantification of Nrf2. HK-2 cells were transfected with 30 nM Nrf2 siRNA for 48 h and then treated with MCTR1 (100 nM) and LPS (1 ug/ml) for 8 h. C , D Representative western blotting and quantification of Nrf2. E Visualization of cell viability were evaluated by phase-contrast microscopy. F Fold change of cell viability. G , H Representative western blotting and quantification of GPX4 and PTGS2. I – K Quantitative analyses of MDA, GSH, and non-heme iron. L Quantitative analyses of oxidized C11-BODIPY 581/591 probe by flow cytometry. M Representative images of C11-BODIPY 581/591 fluorescent ratio-probe. N Representative TEM images. The black arrow indicates ferroptosis-like mitochondria. O Quantitative analyses of mitochondrial length. n = 3, mean ± SD were presented. **P < 0.01 Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-gpx4-primary-antibodies-wb-testing-2.jpgAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCT116- WT whole cell lysates, Lane 2: human HCT116-GPX4 KO whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (A02059-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 19 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-gpx4-primary-antibodies-ihc-testing-1.jpgAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®IHC analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1). Glutathione Peroxidase 4/GPX4 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glutathione Peroxidase 4/GPX4 Antibody (A02059-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-gpx4-primary-antibodies-ihc-testing-2.jpgAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®IHC analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1). Glutathione Peroxidase 4/GPX4 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glutathione Peroxidase 4/GPX4 Antibody (A02059-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02059-1-gpx4-primary-antibodies-fcm-testing-1.jpgAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1). Overlay histogram showing THP-1 cells stained with A02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 Antibody (A02059-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hsd11b1-picoband-trade-antibody-a01565-boster.html2026-03-17T05:12:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01565-hsd11b1-primary-antibodies-wb-testing-1.jpgAnti-HSD11B1 Antibody Picoband® Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (A01565). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (Catalog # A01565) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ift43-picoband-trade-antibody-a10264-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10264-ift43-primary-antibodies-wb-testing-1.jpgAnti-IFT43 Antibody Picoband® Western blot analysis of IFT43 using anti-IFT43 antibody (A10264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT43 antigen affinity purified polyclonal antibody (Catalog # A10264) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT43 at approximately 23 kDa. The expected band size for IFT43 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10264-ift43-primary-antibodies-wb-testing-2.jpgAnti-IFT43 Antibody Picoband® Western blot analysis of IFT43 using anti-IFT43 antibody (A10264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT43 antigen affinity purified polyclonal antibody (Catalog # A10264) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT43 at approximately 23 kDa. The expected band size for IFT43 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10264-ift43-primary-antibodies-if-testing-3.jpgAnti-IFT43 Antibody Picoband® IF analysis of IFT43 using anti-IFT43 antibody (A10264). IFT43 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IFT43 Antibody (A10264) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10264-ift43-primary-antibodies-fcm-testing-4.pngAnti-IFT43 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-IFT43 antibody (A10264). Overlay histogram showing Caco-2 cells stained with A10264 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT43 Antibody (A10264, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-insulin-receptor-insr-picoband-trade-antibody-a00447-2-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00447-2-insr-primary-antibodies-wb-testing-1.jpgAnti-Insulin Receptor/INSR Antibody Picoband® Western blot analysis of Insulin Receptor/INSR using anti-Insulin Receptor/INSR antibody (A00447-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insulin Receptor/INSR antigen affinity purified polyclonal antibody (Catalog # A00447-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insulin Receptor/INSR at approximately 156 kDa. The expected band size for Insulin Receptor/INSR is at 156 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-kif7-picoband-trade-antibody-a04321-3-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04321-3-kif7-primary-antibodies-wb-testing-1.jpgAnti-KIF7 Antibody Picoband® Western blot analysis of KIF7 using anti-KIF7 antibody (A04321-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF7 antigen affinity purified polyclonal antibody (Catalog # A04321-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF7 at approximately 151 kDa. The expected band size for KIF7 is at 151 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-madcam1-picoband-trade-antibody-a04227-3-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04227-3-madcam1-primary-antibodies-wb-testing-1.jpgAnti-MADCAM1 Antibody Picoband® Western blot analysis of MADCAM1 using anti-MADCAM1 antibody (A04227-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADCAM1 antigen affinity purified polyclonal antibody (Catalog # A04227-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADCAM1 at approximately 50 kDa. The expected band size for MADCAM1 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-map1lc3a-picoband-trade-antibody-a01543-2-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01543-2-map1lc3a-primary-antibodies-wb-testing-1.jpgAnti-MAP1LC3A Antibody Picoband® Western blot analysis of MAP1LC3A using anti-MAP1LC3A antibody (A01543-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A antigen affinity purified polyclonal antibody (Catalog # A01543-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP1LC3A at approximately 18 kDa. The expected band size for MAP1LC3A is at 14 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-mek1-map2k1-picoband-trade-antibody-a00292-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00292-map2k1-primary-antibodies-wb-testing-1_1.jpgAnti-MEK1/MAP2K1 Antibody Picoband® Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (A00292) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00292-map2k1-primary-antibodies-wb-testing-2.jpgAnti-MEK1/MAP2K1 Antibody Picoband® Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (A00292) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00292-map2k1-primary-antibodies-ihc-testing-1.jpgAnti-MEK1/MAP2K1 Antibody Picoband®IHC analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). MEK1/MAP2K1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MEK1/MAP2K1 Antibody (A00292) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00292-map2k1-primary-antibodies-if-testing-3.jpgAnti-MEK1/MAP2K1 Antibody Picoband® IF analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). MEK1/MAP2K1 was detected in an immunocytochemical section of TPC1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MEK1/MAP2K1 Antibody (A00292) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00292-map2k1-primary-antibodies-if-testing-4.jpgAnti-MEK1/MAP2K1 Antibody Picoband® IF analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). MEK1/MAP2K1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MEK1/MAP2K1 Antibody (A00292) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00292-map2k1-primary-antibodies-fcm-testing-5.jpgAnti-MEK1/MAP2K1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-MEK1/MAP2K1 antibody (A00292). Overlay histogram showing A431 cells stained with A00292 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK1/MAP2K1 Antibody (A00292, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-p38-alpha-mapk14-picoband-trade-antibody-a00176-2-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-wb-testing-1_1.jpgAnti-p38 alpha/MAPK14 Antibody Picoband® Western blot analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p38 alpha/MAPK14 antigen affinity purified polyclonal antibody (A00176-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p38 alpha/MAPK14 at approximately 38 kDa. The expected band size for p38 alpha/MAPK14 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-wb-testing-2.jpgAnti-p38 alpha/MAPK14 Antibody Picoband®Western blot analysis of p38 alpha using anti-p38 alpha antibody (A00176-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p38 alpha antigen affinity purified polyclonal antibody (A00176-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p38 alpha at approximately 41 kDa. The expected band size for p38 alpha is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-ihc-testing-2.jpgAnti-p38 alpha/MAPK14 Antibody Picoband® IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). p38 alpha/MAPK14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p38 alpha/MAPK14 Antibody (A00176-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-ihc-testing-3.jpgAnti-p38 alpha/MAPK14 Antibody Picoband® IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). p38 alpha/MAPK14 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p38 alpha/MAPK14 Antibody (A00176-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-ihc-testing-4.jpgAnti-p38 alpha/MAPK14 Antibody Picoband® IHC analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2). p38 alpha/MAPK14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p38 alpha/MAPK14 Antibody (A00176-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-if-testing-1.jpgAnti-p38 alpha/MAPK14 Antibody Picoband®IF analysis of P38 Alpha/MAPK14 using anti-P38 Alpha/MAPK14 antibody (A00176-2) and anti-Alpha Tubulin antibody (M03989-3). P38 Alpha/MAPK14 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P38 Alpha/MAPK14 Antibody (A00176-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-ip-testing-5.jpgAnti-p38 alpha/MAPK14 Antibody Picoband® Immunoprecipitating p38 alpha/MAPK14 in Jurkat whole cell lysate. Western blot analysis of p38 alpha/MAPK14 using anti-p38 alpha/MAPK14 antibody (A00176-2); Lane 1: Jurkat whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-p38 alpha/MAPK14 antibody in Jurkat whole cell lysate; Lane 3: anti-p38 alpha/MAPK14 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p38 alpha/MAPK14 antigen affinity purified polyclonal antibody (A00176-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for p38 alpha/MAPK14 at approximately 38 kDa. The expected band size for p38 alpha/MAPK14 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-mapk14-primary-antibodies-fcm-testing-6.jpgAnti-p38 alpha/MAPK14 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-P38 Alpha/MAPK14 antibody (A00176-2). Overlay histogram showing CACO-2 cells stained with A00176-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P38 Alpha/MAPK14 Antibody (A00176-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00176-2-fphar-12-594833-g008.jpgAnti-p38 alpha/MAPK14 Antibody Picoband®(A) Protein expression levels of LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK in SMMC-7721 cells measured by Western blot after treatment with 5 μM PL, 10 μM 3-MA autophagy inhibitor, 10 μM rapamycin autophagy agonist, 10 μM SB202190, and 10 μM SB203580 p38 MAPK inhibitor. (B) LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK were used as internal reference total proteins for gray value analysis. * p < 0.05, ** p < 0.01 *** p < 0.001, compared with the PL group. # p < 0.05, ## p < 0.01 ### p < 0.001 vs. the control group. (C) Protein expression levels of LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK in BEL-7404 cells measured by Western blot after treatment with 5 μM PL, 10 μM 3-MA autophagy inhibitor, 10 μM rapamycin autophagy agonist, 10 μM SB202190, and 10 μM SB203580 p38 MAPK inhibitor. (D) LC3-I, LC3-II, p38 MAPK, and p-p38 MAPK were used as internal reference total proteins for gray value analysis. * p < 0.05, ** p < 0.01 *** p < 0.001, compared with the PL group. # p < 0.05, ## p < 0.01 ### p < 0.001 vs. the control group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fphar.2021.594833/full'>33912033</a> https://www.bosterbio.com/products/primary-antibodies/anti-me1-picoband-trade-antibody-a03449-3-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03449-3-me1-primary-antibodies-wb-testing-1_1.jpgAnti-ME1 Antibody Picoband®Western blot analysis of ME1 using anti-ME1 antibody (A03449-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME1 antigen affinity purified polyclonal antibody (A03449-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03449-3-me1-primary-antibodies-wb-testing-12.pngAnti-ME1 Antibody Picoband®Western blot analysis of ME1 using anti-ME1 antibody (A03449-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: LPS-Stimulated human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ME1 antigen affinity purified polyclonal antibody (A03449-3) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03449-3-me1-primary-antibodies-ihc-testing-1.jpgAnti-ME1 Antibody Picoband®IHC analysis of ME1 using anti-ME1 antibody (A03449-3). ME1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ME1 Antibody (A03449-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03449-3-me1-primary-antibodies-if-testing-1.jpgAnti-ME1 Antibody Picoband®IF analysis of ME1 using anti-ME1 antibody (A03449-3) and anti-Tubulin Alpha antibody (M03989-3). ME1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ME1 Antibody (A03449-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03449-3-me1-primary-antibodies-if-testing-2_1.jpgAnti-ME1 Antibody Picoband®IF analysis of ME1 using anti-ME1 antibody (A03449-3). ME1 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ME1 Antibody (A03449-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03449-3-me1-primary-antibodies-fcm-testing-1_1.jpgAnti-ME1 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-ME1 antibody (A03449-3). Overlay histogram showing A549 cells stained with A03449-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ME1 Antibody (A03449-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mlh1-picoband-trade-antibody-a00126-1-boster.html2026-03-17T05:12:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00126-1-mlh1-primary-antibodies-wb-testing-1_1.jpgAnti-MLH1 Antibody Picoband® Western blot analysis of MLH1 using anti-MLH1 antibody (A00126-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody (Catalog # A00126-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85 kDa. The expected band size for MLH1 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00126-1-mlh1-primary-antibodies-ihc-testing-2.jpgAnti-MLH1 Antibody Picoband® IHC analysis of MLH1 using anti-MLH1 antibody (A00126-1). MLH1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MLH1 Antibody (A00126-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00126-1-mlh1-primary-antibodies-ihc-testing-3.jpgAnti-MLH1 Antibody Picoband® IHC analysis of MLH1 using anti-MLH1 antibody (A00126-1). MLH1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MLH1 Antibody (A00126-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00126-1-mlh1-primary-antibodies-ihc-testing-4.jpgAnti-MLH1 Antibody Picoband® IHC analysis of MLH1 using anti-MLH1 antibody (A00126-1). MLH1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MLH1 Antibody (A00126-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00126-1-mlh1-primary-antibodies-ihc-testing-5.jpgAnti-MLH1 Antibody Picoband® IHC analysis of MLH1 using anti-MLH1 antibody (A00126-1). MLH1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MLH1 Antibody (A00126-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-mkl1-mrtfa-picoband-trade-antibody-a32468-2-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32468-2-mrtfa-primary-antibodies-wb-testing-1.jpgAnti-Mkl1/MRTFA Antibody Picoband® Western blot analysis of Mkl1/MRTFA using anti-Mkl1/MRTFA antibody (A32468-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mkl1/MRTFA antigen affinity purified polyclonal antibody (Catalog # A32468-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mkl1/MRTFA at approximately 145 kDa. The expected band size for Mkl1/MRTFA is at 145 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32468-2-mrtfa-primary-antibodies-if-testing-2.jpgAnti-Mkl1/MRTFA Antibody Picoband® IF analysis of Mkl1/MRTFA using anti-Mkl1/MRTFA antibody (A32468-2). Mkl1/MRTFA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Mkl1/MRTFA Antibody (A32468-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-msh2-picoband-trade-antibody-a00140-2-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00140-2-msh2-primary-antibodies-wb-testing-1.jpgAnti-MSH2 Antibody Picoband® Western blot analysis of MSH2 using anti-MSH2 antibody (A00140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # A00140-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105 kDa. The expected band size for MSH2 is at 105 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ncald-picoband-trade-antibody-a09890-2-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09890-2-ncald-primary-antibodies-wb-testing-1_1.jpgAnti-NCALD Antibody Picoband®Western blot analysis of NCALD using anti-NCALD antibody (A09890-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCALD antigen affinity purified polyclonal antibody (Catalog # A09890-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCALD at approximately 18-20 kDa. The expected band size for NCALD is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-nfat4-nf-atc3-nfatc3-picoband-trade-antibody-a02727-3-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02727-3-nfatc3-primary-antibodies-wb-testing-1.jpgAnti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband® Western blot analysis of NFAT4/NF-ATc3/NFATC3 using anti-NFAT4/NF-ATc3/NFATC3 antibody (A02727-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4/NF-ATc3/NFATC3 antigen affinity purified polyclonal antibody (Catalog # A02727-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4/NF-ATc3/NFATC3 at approximately 150 kDa. The expected band size for NFAT4/NF-ATc3/NFATC3 is at 116 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-npl4-nploc4-picoband-trade-antibody-a08476-1-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08476-1-nploc4-primary-antibodies-wb-testing-1.jpgAnti-NPL4/NPLOC4 Antibody Picoband® Western blot analysis of NPL4/NPLOC4 using anti-NPL4/NPLOC4 antibody (A08476-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPL4/NPLOC4 antigen affinity purified polyclonal antibody (Catalog # A08476-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPL4/NPLOC4 at approximately 72 kDa. The expected band size for NPL4/NPLOC4 is at 68 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08476-1-nploc4-primary-antibodies-if-testing-2.jpgAnti-NPL4/NPLOC4 Antibody Picoband® IF analysis of NPL4/NPLOC4 using anti-NPL4/NPLOC4 antibody (A08476-1). NPL4/NPLOC4 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NPL4/NPLOC4 Antibody (A08476-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-p2x4-p2rx4-picoband-trade-antibody-a04715-2-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04715-2-p2rx4-primary-antibodies-wb-testing-1.jpgAnti-P2X4/P2RX4 Antibody Picoband® Western blot analysis of P2X4/P2RX4 using anti-P2X4/P2RX4 antibody (A04715-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X4/P2RX4 antigen affinity purified polyclonal antibody (Catalog # A04715-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X4/P2RX4 at approximately 70 kDa. The expected band size for P2X4/P2RX4 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ppfia1-picoband-trade-antibody-a05735-1-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05735-1-pppfia1-primary-antibodies-wb-testing-1.jpgAnti-PPFIA1 Antibody Picoband® Western blot analysis of PPFIA1 using anti-PPFIA1 antibody (A05735-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPFIA1 antigen affinity purified polyclonal antibody (Catalog # A05735-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPFIA1 at approximately 160 kDa. The expected band size for PPFIA1 is at 136 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05735-1-pppfia1-primary-antibodies-ihc-testing-2.jpgAnti-PPFIA1 Antibody Picoband® IHC analysis of PPFIA1 using anti-PPFIA1 antibody (A05735-1). PPFIA1 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPFIA1 Antibody (A05735-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05735-1-pppfia1-primary-antibodies-ihc-testing-3.jpgAnti-PPFIA1 Antibody Picoband® IHC analysis of PPFIA1 using anti-PPFIA1 antibody (A05735-1). PPFIA1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPFIA1 Antibody (A05735-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05735-1-pppfia1-primary-antibodies-ihc-testing-4.jpgAnti-PPFIA1 Antibody Picoband® IHC analysis of PPFIA1 using anti-PPFIA1 antibody (A05735-1). PPFIA1 was detected in a paraffin-embedded section of human rectal differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPFIA1 Antibody (A05735-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05735-1-pppfia1-primary-antibodies-if-testing-5.jpgAnti-PPFIA1 Antibody Picoband® IF analysis of PPFIA1 using anti-PPFIA1 antibody (A05735-1). PPFIA1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPFIA1 Antibody (A05735-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rad18-picoband-trade-antibody-a01622-2-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01622-2-rad18-primary-antibodies-wb-testing-1.jpgAnti-RAD18 Antibody Picoband® Western blot analysis of RAD18 using anti-RAD18 antibody (A01622-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD18 antigen affinity purified polyclonal antibody (Catalog # A01622-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD18 at approximately 75 kDa. The expected band size for RAD18 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01622-2-rad18-primary-antibodies-ihc-testing-2.jpgAnti-RAD18 Antibody Picoband® IHC analysis of RAD18 using anti-RAD18 antibody (A01622-2). RAD18 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD18 Antibody (A01622-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01622-2-rad18-primary-antibodies-ihc-testing-3.jpgAnti-RAD18 Antibody Picoband® IHC analysis of RAD18 using anti-RAD18 antibody (A01622-2). RAD18 was detected in a paraffin-embedded section of squamous metaplasia of human renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD18 Antibody (A01622-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01622-2-rad18-primary-antibodies-ihc-testing-4.jpgAnti-RAD18 Antibody Picoband® IHC analysis of RAD18 using anti-RAD18 antibody (A01622-2). RAD18 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD18 Antibody (A01622-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01622-2-rad18-primary-antibodies-fcm-testing-5.pngAnti-RAD18 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-RAD18 antibody (A01622-2). Overlay histogram showing K562 cells stained with A01622-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD18 Antibody (A01622-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rpl32-picoband-trade-antibody-a06487-1-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-wb-testing-1.jpgAnti-RPL32 Antibody Picoband® Western blot analysis of RPL32 using anti-RPL32 antibody (A06487-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL32 antigen affinity purified polyclonal antibody (Catalog # A06487-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL32 at approximately 19 kDa. The expected band size for RPL32 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-ihc-testing-2.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-1). RPL32 was detected in a paraffin-embedded section of differentiated adenocarcinoma of the human rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-ihc-testing-3.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-1). RPL32 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-ihc-testing-4.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-1). RPL32 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-ihc-testing-5.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-1). RPL32 was detected in a paraffin-embedded section of squamous metaplasia of human renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-if-testing-6.jpgAnti-RPL32 Antibody Picoband® IF analysis of RPL32 using anti-RPL32 antibody (A06487-1). RPL32 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPL32 Antibody (A06487-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-fcm-testing-7.pngAnti-RPL32 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-RPL32 antibody (A06487-1). Overlay histogram showing C6 cells stained with A06487-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL32 Antibody (A06487-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-1-rpl32-primary-antibodies-fcm-testing-8.pngAnti-RPL32 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RPL32 antibody (A06487-1). Overlay histogram showing HL-60 cells stained with A06487-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL32 Antibody (A06487-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snon-sno-skil-picoband-trade-antibody-a04131-2-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-2-skil-primary-antibodies-wb-testing-1.jpgAnti-SnoN/SNO/SKIL Antibody Picoband® Western blot analysis of SnoN/SNO/SKIL using anti-SnoN/SNO/SKIL antibody (A04131-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Sw620 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SnoN/SNO/SKIL antigen affinity purified polyclonal antibody (Catalog # A04131-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SnoN/SNO/SKIL at approximately 77 kDa. The expected band size for SnoN/SNO/SKIL is at 77 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-2-skil-primary-antibodies-ihc-testing-2.jpgAnti-SnoN/SNO/SKIL Antibody Picoband® IHC analysis of SnoN/SNO/SKIL using anti-SnoN/SNO/SKIL antibody (A04131-2). SnoN/SNO/SKIL was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SnoN/SNO/SKIL Antibody (A04131-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-2-skil-primary-antibodies-ihc-testing-3.jpgAnti-SnoN/SNO/SKIL Antibody Picoband® IHC analysis of SnoN/SNO/SKIL using anti-SnoN/SNO/SKIL antibody (A04131-2). SnoN/SNO/SKIL was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SnoN/SNO/SKIL Antibody (A04131-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-2-skil-primary-antibodies-if-testing-4.jpgAnti-SnoN/SNO/SKIL Antibody Picoband® IF analysis of SnoN/SNO/SKIL using anti-SnoN/SNO/SKIL antibody (A04131-2). SnoN/SNO/SKIL was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SnoN/SNO/SKIL Antibody (A04131-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 555 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04131-2-skil-primary-antibodies-fcm-testing-5.pngAnti-SnoN/SNO/SKIL Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SnoN/SNO/SKIL antibody (A04131-2). Overlay histogram showing 293T cells stained with A04131-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SnoN/SNO/SKIL Antibody (A04131-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sdf2-picoband-trade-antibody-a12906-1-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12906-1-sdf2-primary-antibodies-wb-testing-1.jpgAnti-SDF2 Antibody Picoband® Western blot analysis of SDF2 using anti-SDF2 antibody (A12906-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDF2 antigen affinity purified polyclonal antibody (Catalog # A12906-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDF2 at approximately 23 kDa. The expected band size for SDF2 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-snap23-picoband-trade-antibody-a02487-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-snap23-primary-antibodies-wb-testing-1.jpgAnti-SNAP23 Antibody Picoband® Western blot analysis of SNAP23 using anti-SNAP23 antibody (A02487). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP23 antigen affinity purified polyclonal antibody (Catalog # A02487) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP23 at approximately 23 kDa. The expected band size for SNAP23 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-snap23-primary-antibodies-ihc-testing-2.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487). SNAP23 was detected in a paraffin-embedded section of differentiated adenocarcinoma of the human rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-snap23-primary-antibodies-ihc-testing-3.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487). SNAP23 was detected in a paraffin-embedded section of human gastric signet ring cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-snap23-primary-antibodies-ihc-testing-4.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487). SNAP23 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-snap23-primary-antibodies-ihc-testing-5.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487). SNAP23 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-snap23-primary-antibodies-fcm-testing-6.pngAnti-SNAP23 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SNAP23 antibody (A02487). Overlay histogram showing SiHa cells stained with A02487 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP23 Antibody (A02487, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sun2-picoband-trade-antibody-a03291-1-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-wb-testing-1.jpgAnti-SUN2 Antibody Picoband® Western blot analysis of SUN2 using anti-SUN2 antibody (A03291-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUN2 antigen affinity purified polyclonal antibody (Catalog # A03291-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUN2 at approximately 80 kDa. The expected band size for SUN2 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-ihc-testing-2.jpgAnti-SUN2 Antibody Picoband® IHC analysis of SUN2 using anti-SUN2 antibody (A03291-1). SUN2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUN2 Antibody (A03291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-ihc-testing-3.jpgAnti-SUN2 Antibody Picoband® IHC analysis of SUN2 using anti-SUN2 antibody (A03291-1). SUN2 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUN2 Antibody (A03291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-ihc-testing-4.jpgAnti-SUN2 Antibody Picoband® IHC analysis of SUN2 using anti-SUN2 antibody (A03291-1). SUN2 was detected in a paraffin-embedded section of human infiltrating adenocarcinoma of the lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUN2 Antibody (A03291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-ihc-testing-5.jpgAnti-SUN2 Antibody Picoband® IHC analysis of SUN2 using anti-SUN2 antibody (A03291-1). SUN2 was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUN2 Antibody (A03291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-ihc-testing-6.jpgAnti-SUN2 Antibody Picoband® IHC analysis of SUN2 using anti-SUN2 antibody (A03291-1). SUN2 was detected in a paraffin-embedded section of human renal pelvis is squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUN2 Antibody (A03291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-ihc-testing-7.jpgAnti-SUN2 Antibody Picoband® IHC analysis of SUN2 using anti-SUN2 antibody (A03291-1). SUN2 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUN2 Antibody (A03291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03291-1-sun2-primary-antibodies-if-testing-8.jpgAnti-SUN2 Antibody Picoband® IF analysis of SUN2 using anti-SUN2 antibody (A03291-1). SUN2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUN2 Antibody (A03291-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tgf-beta-1-tgfb1-picoband-trade-antibody-a00019-2-boster.html2026-03-17T05:12:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-2-tgfb1-primary-antibodies-ihc-testing-1.jpgAnti-TGF beta 1/TGFB1 Antibody IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (A00019-2). TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TGF Beta 1/TGFB1 Antibody (A00019-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-2-41598_2018_27787_fig1_html.jpgAnti-TGF beta 1/TGFB1 AntibodyThe representative samples of immunohistochemical staining for AGE, RAGE, TGF-β1, TGF- β1 receptor, BDNF and TrkB in the colon wall of two groups. The microscopy with high magnification have been inserted in each single histological photo (arrow) in order to display the localization of markers. The staining of all proteins was stronger in the muscle layer than other layers. In the different layers, the staining of AGE, RAGE, TGF-β1 and TGF- β1 receptor was stronger whereas the staining of BDNF and TrkB was weaker in the Diabetes group than in Control group. Bar = 100 um. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-2-41598_2018_27787_fig2_html.jpgAnti-TGF beta 1/TGFB1 AntibodyThe fraction of AGE, RAGE, TGF-β1, TGF- β1 receptor, BDNF and TrkB in the different layers of the colon between two groups. In the different layers, the fraction of AGE, RAGE, TGF-β1 and TGF- β1 receptor was bigger whereas the fraction of BDNF and TrkB was smaller in the Diabetes group than in Control group. Compared with Control group: *P < 0.05, **P < 0.01. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-2-41598_2018_27787_fig3_html.jpgAnti-TGF beta 1/TGFB1 Antibody( A ) Correlation between AGE and RAGE in muscle layer and submucosa layer with circumferential constant a; ( B ) Correlation between AGE and RAGE in muscle layer and submucosa layer with longitudinal constant a; ( C ) Correlation between TGF-β1 and TGF-β1 receptor in mucosa layer and TGF-β1 muscle layer with circumferential and longitudinal material constant a; ( D ) Correlation between BDNF in muscle and submucosa layers with longitudinal constant a. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-2-41598_2018_27787_fig4_html.jpgAnti-TGF beta 1/TGFB1 Antibody( A ) Correlation between AGE and RAGE in different layers; ( B ) Correlation between TGF-β1 and TGF-β1receptor in different layers; ( C ) Correlation between BDNF and TrkB in different layers; ( D ) Correlation between RAGE and TGF-β1receptor in different layers. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a> https://www.bosterbio.com/products/primary-antibodies/anti-tgf-beta-receptor-iii-tgfbr3-picoband-trade-antibody-a02765-4-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-4-tgfbr3-primary-antibodies-wb-testing-1.jpgAnti-TGF beta Receptor III/TGFBR3 Antibody Picoband® Western blot analysis of TGF Beta Receptor III/TGFBR3 using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat L6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 antigen affinity purified polyclonal antibody (Catalog # A02765-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor III/TGFBR3 at approximately 93 kDa. The expected band size for TGF Beta Receptor III/TGFBR3 is at 93 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tlr8-picoband-trade-antibody-a01541-4-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01541-4-tlr8-primary-antibodies-wb-testing-1.jpgAnti-Tlr8 Antibody Picoband® Western blot analysis of Tlr8 using anti-Tlr8 antibody (A01541-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr8 antigen affinity purified polyclonal antibody (Catalog # A01541-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr8 at approximately 119 kDa. The expected band size for Tlr8 is at 119 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01541-4-tlr8-primary-antibodies-ihc-testing-2.jpgAnti-Tlr8 Antibody Picoband® IHC analysis of Tlr8 using anti-Tlr8 antibody (A01541-4). Tlr8 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tlr8 Antibody (A01541-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01541-4-tlr8-primary-antibodies-ihc-testing-3.jpgAnti-Tlr8 Antibody Picoband® IHC analysis of Tlr8 using anti-Tlr8 antibody (A01541-4). Tlr8 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tlr8 Antibody (A01541-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-tomm70-picoband-trade-antibody-a09172-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-wb-testing-1.jpgAnti-TOMM70 Antibody Picoband® Western blot analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM70 antigen affinity purified polyclonal antibody (Catalog # A09172-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM70 at approximately 67 kDa. The expected band size for TOMM70 is at 67 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-ihc-testing-2.jpgAnti-TOMM70 Antibody Picoband® IHC analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). TOMM70 was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM70 Antibody (A09172-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-ihc-testing-3.jpgAnti-TOMM70 Antibody Picoband® IHC analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). TOMM70 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM70 Antibody (A09172-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-ihc-testing-4.jpgAnti-TOMM70 Antibody Picoband® IHC analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). TOMM70 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM70 Antibody (A09172-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-ihc-testing-5.jpgAnti-TOMM70 Antibody Picoband® IHC analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). TOMM70 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM70 Antibody (A09172-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-ihc-testing-6.jpgAnti-TOMM70 Antibody Picoband® IHC analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). TOMM70 was detected in a paraffin-embedded section of human a differentiated adenocarcinoma of the rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM70 Antibody (A09172-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-if-testing-7.jpgAnti-TOMM70 Antibody Picoband® IF analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). TOMM70 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOMM70 Antibody (A09172-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09172-2-tomm70-primary-antibodies-fcm-testing-8.pngAnti-TOMM70 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-TOMM70 antibody (A09172-2). Overlay histogram showing Caco-2 cells stained with A09172-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM70 Antibody (A09172-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tryptophan-hydroxylase-tph1-picoband-trade-antibody-a01626-4-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-4-tph1-primary-antibodies-wb-testing-1.jpgAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® Western blot analysis of Tryptophan Hydroxylase/TPH1 using anti-Tryptophan Hydroxylase/TPH1 antibody (A01626-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase/TPH1 antigen affinity purified polyclonal antibody (Catalog # A01626-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase/TPH1 at approximately 60 kDa. The expected band size for Tryptophan Hydroxylase/TPH1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-4-tph1-primary-antibodies-ihc-testing-5.jpgAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®IHC analysis of TPH1 using anti-TPH1 antibody (A01626-4). TPH1 was detected in a paraffin-embedded section of human small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPH1 Antibody (A01626-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-4-tph1-primary-antibodies-ihc-testing-2.jpgAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® IHC analysis of Tryptophan Hydroxylase/TPH1 using anti-Tryptophan Hydroxylase/TPH1 antibody (A01626-4). Tryptophan Hydroxylase/TPH1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tryptophan Hydroxylase/TPH1 Antibody (A01626-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-4-tph1-primary-antibodies-ihc-testing-3.jpgAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® IHC analysis of Tryptophan Hydroxylase/TPH1 using anti-Tryptophan Hydroxylase/TPH1 antibody (A01626-4). Tryptophan Hydroxylase/TPH1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tryptophan Hydroxylase/TPH1 Antibody (A01626-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-4-tph1-primary-antibodies-ihc-testing-4.jpgAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® IHC analysis of Tryptophan Hydroxylase/TPH1 using anti-Tryptophan Hydroxylase/TPH1 antibody (A01626-4). Tryptophan Hydroxylase/TPH1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tryptophan Hydroxylase/TPH1 Antibody (A01626-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-4-tph1-primary-antibodies-if-testing-5.jpgAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® IF analysis of Tryptophan Hydroxylase/TPH1 using anti-Tryptophan Hydroxylase/TPH1 antibody (A01626-4). Tryptophan Hydroxylase/TPH1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tryptophan Hydroxylase/TPH1 Antibody (A01626-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01626-4-tph1-primary-antibodies-fcm-testing-6.pngAnti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-Tryptophan Hydroxylase/TPH1 antibody (A01626-4). Overlay histogram showing HEL cells stained with A01626-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tryptophan Hydroxylase/TPH1 Antibody (A01626-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-vitamin-d-receptor-vdr-picoband-trade-antibody-a00210-1-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00210-1-vdr-primary-antibodies-wb-testing-1.jpgAnti-Vitamin D Receptor/VDR Antibody Picoband® Western blot analysis of Vitamin D Receptor/VDR using anti-Vitamin D Receptor/VDR antibody (A00210-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitamin D Receptor/VDR antigen affinity purified polyclonal antibody (Catalog # A00210-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitamin D Receptor/VDR at approximately 60 kDa. The expected band size for Vitamin D Receptor/VDR is at 60 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00210-1-vdr-primary-antibodies-fcm-testing-2_1.pngAnti-Vitamin D Receptor/VDR Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Vitamin D Receptor/VDR antibody (A00210-1). Overlay histogram showing C6 cells stained with A00210-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Vitamin D Receptor/VDR Antibody (A00210-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00210-1-vdr-primary-antibodies-fcm-testing-3_1.pngAnti-Vitamin D Receptor/VDR Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-Vitamin D Receptor/VDR antibody (A00210-1). Overlay histogram showing U20S cells stained with A00210-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Vitamin D Receptor/VDR Antibody (A00210-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/picokine-elisa-kits/human-scd2-picokine-elisa-kit-ek2158-boster.html2026-03-10T04:33:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2158.jpgHuman sCD2 ELISA Kit PicoKine®Human sCD2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd34-picokine-elisa-kit-ek2159-boster.html2026-03-10T04:33:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2159.jpgHuman CD34 ELISA Kit PicoKine®Human CD34 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cdsn-picokine-elisa-kit-ek2160-boster.html2026-03-10T04:33:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2160.pngHuman CDSN ELISA Kit PicoKine®Human CDSN PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dnam-1-cd226-picokine-elisa-kit-ek2161-boster.html2026-03-10T04:33:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2161.jpgHuman DNAM-1/CD226 ELISA Kit PicoKine®Human DNAM-1/CD226 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-langerin-cd207-picokine-elisa-kit-ek2162-boster.html2026-03-10T04:33:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2162.pngHuman Langerin/CD207 ELISA Kit PicoKine®Human Langerin/CD207 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/all-secondary-antibodies/fluoro-488-conjugated-affinipure-rabbit-igg-ba1045-fluoro488-boster.html2026-03-10T04:33:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_16.pngFluoro488 Conjugated AffiniPure Rabbit IgG (H+L)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/anti-adam9-picoband-trade-antibody-a03074-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03074-2-adam9-primary-antibodies-wb-testing-1.jpgAnti-ADAM9 Antibody Picoband® Western blot analysis of ADAM9 using anti-ADAM9 antibody (A03074-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human Hacat whole cell lysates, Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM9 antigen affinity purified polyclonal antibody (Catalog # A03074-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM9 at approximately 75 kDa. The expected band size for ADAM9 is at 90 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03074-2-adam9-primary-antibodies-wb-testing-2.jpgAnti-ADAM9 Antibody Picoband® Western blot analysis of ADAM9 using anti-ADAM9 antibody (A03074-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM9 antigen affinity purified polyclonal antibody (Catalog # A03074-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM9 at approximately 75 kDa. The expected band size for ADAM9 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03074-2-adam9-primary-antibodies-fcm-testing-3.pngAnti-ADAM9 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-ADAM9 antibody (A03074-2). Overlay histogram showing U251 cells stained with A03074-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAM9 Antibody (A03074-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-akt1-2-3-picoband-trade-antibody-a00024-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-12933_2013_article_642_fig2_html.jpgAnti-AKT1,2,3 Antibody Picoband®HPA, RAGE and PI3K/AKT pathway correlate with AGEs-induced macrophage migration. Cells were cultured with AGEs for 24 h with or without pre-treatment with LY294002, anti-HPA or RAGE antibody for 1 h. The migration was measured by transwell assays. Results were normalized to the number of macrophages that migrated in control group. The results represent the mean of six culture wells (mean ± SEM). *p < 0.05 compared to control and #p <0.05 compared to AGEs. All of the experiments were performed independently in triplicate. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1475-2840-12-37'>23442498</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-12933_2013_article_642_fig4_html.jpgAnti-AKT1,2,3 Antibody Picoband®The expression of AKT protein in AGEs-induced macrophages. Cells were cultured with AGEs or pretreated with antibody against RAGE or HPA for 1 h before exposed to AGEs for 24 h. AKT and p-AKT protein expression is determined by Western blot analysis using anti-AKT and p-AKT antibody. The results represent the mean of six culture wells (mean ± SEM). * P < 0.05 compared to control and # P <0.05 compared to AGEs. All of the experiments were performed independently in triplicate. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1475-2840-12-37'>23442498</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-fig-5-1x.jpgAnti-AKT1,2,3 Antibody Picoband®Expression change of PTEN/PI3K/Akt pathway in the process of miR-21 mimics induced proliferation in c-kit + CSCs. Cultured CSCs were treated with miR-21 mimics for 48 h before the subsequent procedures. To test the contribution of PTEN/PI3K/Akt signaling to miR-21 mimics’s pro-proliferation effects in c-kit + CSCs, PTEN and PI3K were inhibited with Phen or LY294002 respectively. (A) RT-PCR was carried out to detect miR-21 mimics’s effects on PTEN expression at the mRNA level, which showed no change between Control, miR-21 scramble, miR-21 mimics and miR-21 mimics+ LY294002 group, while Phen resulted in a significant down-regulation of PTEN compared with the other groups. (B–C) Western blot was carried out to detect miR-21 mimics’s effects on PTEN protein expression, which showed that miR-21 mimics significantly down-regulated PTEN protein in miR-21 mimics group compared with the scramble group. In addition, both Phen treatment and miR-21 mimics incubation increased p-Akt level, while PI3K inhibitor LY294002 decreased p-Akt level dramatically ( P < 0.05). a, P < 0.05 compared with Control; b, P < 0.05 compared with miR-21 scramble group; c, P < 0.05 compared with miR-21 mimics group; d, P < 0.05 compared with Phen group. n = 3 in each group. p-Akt = phosphor-Akt. Download full-size image DOI: Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/2859/'>28168101</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-pm-12-139-g007.jpgAnti-AKT1,2,3 Antibody Picoband®Effect of treadmill exercise and epicatechin on brain-derived neurotrophic factor levels and Akt/GSK-3/cAMP response element binding protein signaling pathway in the hippocampus. (a) Representative Western blot of protein expressions normalized to β-actin protein in the hippocampus. (b) Protein expressions were semi-quantified by densitometry analysis. Values are expressed as mean ± standard error. * P < 0.05 compared with wild-type littermates; # P < 0.05 compared with transgenic; and P < 0.05 compared with COMA Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4883070/&orig_host=www.ncbi.nlm.nih.gov'>27279698</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-ajtr0009-0247-f8.jpgAnti-AKT1,2,3 Antibody Picoband®Liraglutide promotes activation of the p-Akt/GSK-3β signaling pathway (Western blot). Liraglutide increased the active (phosphorylated) form of Akt (A, B) and decreased the active form of GSK-3β (non-phosphorylated) (A, C). The samples derive from the same experiment and blots were processed in parallel ( n = 6 mice per group). β-Actin was used as the loading control. *P<0.05. Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340664/'>28337257</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-fig-4-1x.jpgAnti-AKT1,2,3 Antibody Picoband®PTEN/PI3K/Akt pathway’s contribution in miR-21 induced proliferation in c-kit + CSCs. Cultured c-kit + CSCs were treated with miR-21 mimics for 48 h before subjected to EdU immunofluorescence (A–B), flow cytometry (C) or Western blot (D). To test the contribution of PTEN/PI3K/Akt signaling, PTEN and PI3K were inhibited with Phen or LY294002 respectively. (A) c-kit + CSCs were double stained by EdU (green) and DAPI (blue), and observed under a fluorescence microscope (Olympus). Bar = 50 µm. DAPI = propidium iodide. (B) The statistics of EdU positive CSCs from immunofluorescence in (A). n = 6 in each group. (C) Flow cytometry was employed to detect cell cycle profiles in CSCs underwent different treatments miR-21 mimics or Phen increased the proportion of S phase CSCs compared with Control or scramble treated groups. n = 3. (D) PTEN/PI3K/Akt pathway’s influences on BrdU expression, which was detected with immune blotting. Just like miR-21 mimics’ effect on BrdU, when PTEN was inhibited by Phen, there was notably increase of BrdU compared with Normal or Scramble group. When PI3K was inhibited by LY294002, there was notably decrease of BrdU in mimics+LY294002 group compared with mimics group in CSCs. n = 3 in each group. a, P < 0.05 compared with Control; b, P < 0.05 compared with Scramble; c, P < 0.05 compared with miR-21 mimics group; d, P < 0.05 compared with Phen group. Download full-size image DOI: Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/2859/'>28168101</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-ajtr0009-0247-f7.jpgAnti-AKT1,2,3 Antibody Picoband®Liraglutide reduces activated (c)-caspase-3 and tau hyperphosphorylation induced by MG (Western blot). A: c-Caspase-3/caspase-3; B: Phospho-tau (Ser202/199)/phospho-tau (Ser396)/tau; C: Phospho-Akt (Ser473)/Akt; D: Phospho-GSK-3β/GSK-3β protein in the hippocampus ( n = 6 mice per group). All samples shown derive from the same experiment and blots were processed in parallel. β-Actin was used as a loading control. *P<0.05. Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340664/'>28337257</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-ajtr0009-0247-f2.jpgAnti-AKT1,2,3 Antibody Picoband®MG induced tau hyperphosphorylation via the GSK-3β pathway (Western blot). Phospho-tau (Ser396), phospho-tau (Ser202/199), tau, phospho-Akt (Ser473)/Akt and phospho-GSK-3β/GSK-3β protein in the hippocampus. Compared with the NS group, the 0.7-µmol MG group showed (A) higher phospho-tau (Ser202/199) and phospho-tau (Ser396) expression; (B) Lower phospho-Akt (Ser473) and phospho-GSK-3β protein expression. Total levels of these proteins were unchanged. The samples derived from the same experiment and blots were processed in parallel. β-actin was used as a loading control. n = 6 mice per group. GSK-3β, glycogen synthase kinase-3β; MG, methylglyoxal; NS, normal saline. Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340664/'>28337257</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-fphar-10-01459-g006.jpgAnti-AKT1,2,3 Antibody Picoband®Effects of magnolol on mice alcohol-induced liver damage in the AKT/PI3K signaling pathway. Liver tissues were extracted for protein analysis by western blotting. AKT and PI3K, proteins expression were detected. The levels of AKT and PI3K were compared with GAPDH. The data were demonstrated as means ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001). Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.01459/full'>31920652</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-ajcr0013-4145-f4.jpgAnti-AKT1,2,3 Antibody Picoband®The inhibition of HGF-MET-Akt pathway in MET-amplified osimertinib-acquired resistant H1975/OR cells by the combination of luteolin and osimertinib. A. Relative MET gene copy numbers in H1975 and H1975/OR cell lines at different luteolin and osimertinib concentrations. B. MET, p-MET, Akt 1,2,3, and p-Akt protein expression in H1975 and H1975/OR cells at different luteolin and osimertinib concentrations. C. HGF protein expression in H1975 and H1975/OR cell lines at different luteolin and osimertinib concentrations. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10560942/&orig_host=www.ncbi.nlm.nih.gov'>37818074</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-atk1_2_3-primary-antibodies-wb-testing-1.jpgAnti-AKT1,2,3 Antibody Picoband® Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-bolan_guan.pngAnti-AKT1,2,3 Antibody Picoband® Western blot analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 4T1 whole cell lysates, Lane 2: LPS-stimulated 4T1 whole cell lysates, Lane 3: Low-dose drug treatment 4T1 whole cell lysates, Lane 4: Medium-dose drug treatment 4T1 whole cell lysates, Lane 5: High-dose drug treatment 4T1 whole cell lysates, Lane 6: Positive control drug treatment 4T1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1,2,3 antigen affinity purified polyclonal antibody (Catalog # A00024-2) at 1:10000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for AKT1,2,3 at approximately 60 kDa. The expected band size for AKT1,2,3 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-atk1_2_3-primary-antibodies-if-testing-2.jpgAnti-AKT1,2,3 Antibody Picoband® IF analysis of AKT1,2,3 using anti-AKT1,2,3 antibody (A00024-2). AKT1,2,3 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AKT1,2,3 Antibody (A00024-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00024-2-atk1_2_3-primary-antibodies-fcm-testing-3_1.pngAnti-AKT1,2,3 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-AKT1,2,3 antibody (A00024-2). Overlay histogram showing Hela cells stained with A00024-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKT1,2,3 Antibody (A00024-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atg4b-picoband-trade-antibody-a02885-1-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02885-1-agt4b-primary-antibodies-wb-testing-1.jpgAnti-ATG4B Antibody Picoband® Western blot analysis of ATG4B using anti-ATG4B antibody (A02885-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat liver tissue lysates, Lane 11: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4B antigen affinity purified polyclonal antibody (Catalog # A02885-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4B at approximately 48 kDa. The expected band size for ATG4B is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02885-1-agt4b-primary-antibodies-fcm-testing-2.pngAnti-ATG4B Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-ATG4B antibody (A02885-1). Overlay histogram showing Jurkat cells stained with A02885-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4B Antibody (A02885-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spg3a-atl1-picoband-trade-antibody-a01994-1-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01994-1-atl1-primary-antibodies-wb-testing-1.jpgAnti-SPG3A/ATL1 Antibody Picoband® Western blot analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPG3A/ATL1 antigen affinity purified polyclonal antibody (Catalog # A01994-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPG3A/ATL1 at approximately 64 kDa. The expected band size for SPG3A/ATL1 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01994-1-atl1-primary-antibodies-ihc-testing-2.jpgAnti-SPG3A/ATL1 Antibody Picoband® IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1). SPG3A/ATL1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01994-1-atl1-primary-antibodies-ihc-testing-3.jpgAnti-SPG3A/ATL1 Antibody Picoband® IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1). SPG3A/ATL1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01994-1-atl1-primary-antibodies-ihc-testing-4.jpgAnti-SPG3A/ATL1 Antibody Picoband® IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1). SPG3A/ATL1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01994-1-atl1-primary-antibodies-ihc-testing-5.jpgAnti-SPG3A/ATL1 Antibody Picoband® IHC analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody (A01994-1). SPG3A/ATL1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPG3A/ATL1 Antibody (A01994-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01994-1-atl1-primary-antibodies-fcm-testing-6.pngAnti-SPG3A/ATL1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SPG3A/ATL1 antibody (A01994-1). Overlay histogram showing U87 cells stained with A01994-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPG3A/ATL1 Antibody (A01994-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atp6v1a-picoband-trade-antibody-a10401-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-wb-testing-1.jpgAnti-ATP6V1A Antibody Picoband® Western blot analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1A antigen affinity purified polyclonal antibody (Catalog # A10401-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP6V1A at approximately 70 kDa. The expected band size for ATP6V1A is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-wb-testing-2.jpgAnti-ATP6V1A Antibody Picoband® Western blot analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat NRK whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1A antigen affinity purified polyclonal antibody (Catalog # A10401-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP6V1A at approximately 70 kDa. The expected band size for ATP6V1A is at 70 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-ihc-testing-3.jpgAnti-ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-ihc-testing-4.jpgAnti-ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-ihc-testing-5.jpgAnti-ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in a paraffin-embedded section of human lienal rupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-ihc-testing-6.jpgAnti-ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-ihc-testing-7.jpgAnti-ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-ihc-testing-8.jpgAnti-ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-ihc-testing-9.jpgAnti-ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-if-testing-10.jpgAnti-ATP6V1A Antibody Picoband® IF analysis of ATP6V1A using anti-ATP6V1A antibody (A10401-2). ATP6V1A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATP6V1A Antibody (A10401-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-fcm-testing-11.pngAnti-ATP6V1A Antibody Picoband® Flow Cytometry analysis of JK cells using anti-ATP6V1A antibody (A10401-2). Overlay histogram showing JK cells stained with A10401-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP6V1A Antibody (A10401-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10401-2-atp6v1a-primary-antibodies-fcm-testing-12.pngAnti-ATP6V1A Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-ATP6V1A antibody (A10401-2). Overlay histogram showing U251 cells stained with A10401-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP6V1A Antibody (A10401-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atr-picoband-trade-antibody-a00262-3-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00262-3-atr-primary-antibodies-wb-testing-1.jpgAnti-ATR Antibody Picoband® Western blot analysis of ATR using anti-ATR antibody (A00262-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATR antigen affinity purified polyclonal antibody (Catalog # A00262-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATR at approximately 301 kDa. The expected band size for ATR is at 301 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00262-3-atr-primary-antibodies-fcm-testing-2.pngAnti-ATR Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-ATR antibody (A00262-3). Overlay histogram showing U87 cells stained with A00262-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATR Antibody (A00262-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-azurocidin-azu1-picoband-trade-antibody-a05212-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05212-1-azu1-primary-antibodies-wb-testing-1.jpgAnti-Azurocidin/AZU1 Antibody Picoband® Western blot analysis of Azurocidin/AZU1 using anti-Azurocidin/AZU1 antibody (A05212-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Azurocidin/AZU1 antigen affinity purified polyclonal antibody (Catalog # A05212-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Azurocidin/AZU1 at approximately 37 kDa. The expected band size for Azurocidin/AZU1 is at 37 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05212-1-azu1-primary-antibodies-ihc-testing-2.jpgAnti-Azurocidin/AZU1 Antibody Picoband® IHC analysis of Azurocidin/AZU1 using anti-Azurocidin/AZU1 antibody (A05212-1). Azurocidin/AZU1 was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Azurocidin/AZU1 Antibody (A05212-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05212-1-azu1-primary-antibodies-fcm-testing-3.pngAnti-Azurocidin/AZU1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Azurocidin/AZU1 antibody (A05212-1). Overlay histogram showing THP-1 cells stained with A05212-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Azurocidin/AZU1 Antibody (A05212-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bap1-picoband-trade-antibody-a00345-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00345-2-bap1-primary-antibodies-wb-testing-1_1.jpgAnti-BAP1 Antibody Picoband®Western blot analysis of BAP1 using anti-BAP1 antibody (A00345-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP1 antigen affinity purified polyclonal antibody (A00345-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BAP1 at approximately 95-100 kDa. The expected band size for BAP1 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00345-2-bap1-primary-antibodies-if-testing-1.jpgAnti-BAP1 Antibody Picoband®IF analysis of BAP1 using anti-BAP1 antibody (A00345-2). BAP1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BAP1 Antibody (A00345-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00345-2-bap1-primary-antibodies-fcm-testing-2.pngAnti-BAP1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-BAP1 antibody (A00345-2). Overlay histogram showing MCF-7 cells stained with A00345-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAP1 Antibody (A00345-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bex3-picoband-trade-antibody-a09675-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09675-2-bex3-primary-antibodies-wb-testing-1.jpgAnti-BEX3 Antibody Picoband® Western blot analysis of BEX3 using anti-BEX3 antibody (A09675-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BEX3 antigen affinity purified polyclonal antibody (Catalog # A09675-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BEX3 at approximately 28 kDa. The expected band size for BEX3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09675-2-bex3-primary-antibodies-fcm-testing-2_1.pngAnti-BEX3 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-BEX3 antibody (A09675-2). Overlay histogram showing MCF-7 cells stained with A09675-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BEX3 Antibody (A09675-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-brd4-picoband-trade-antibody-a00123-3-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00123-3-brd4-primary-antibodies-wb-testing-1_1.jpgAnti-BRD4 Antibody Picoband® Western blot analysis of BRD4 using anti-BRD4 antibody (A00123-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD4 antigen affinity purified polyclonal antibody (Catalog # A00123-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD4 at approximately 240 kDa. The expected band size for BRD4 is at 152 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00123-3-brd4-primary-antibodies-if-testing-2_1.jpgAnti-BRD4 Antibody Picoband® IF analysis of BRD4 using anti-BRD4 antibody (A00123-3). BRD4 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BRD4 Antibody (A00123-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00123-3-brd4-primary-antibodies-fcm-testing-3.jpgAnti-BRD4 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-BRD4 antibody (A00123-3). Overlay histogram showing A549 cells stained with A00123-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRD4 Antibody (A00123-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-c5-picoband-trade-antibody-a00156-1-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00156-1-c5-primary-antibodies-wb-testing-1.jpgAnti-C5 Antibody Picoband® Western blot analysis of C5 using anti-C5 antibody (A00156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse RAW364.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5 antigen affinity purified polyclonal antibody (Catalog # A00156-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5 at approximately 75 kDa. The expected band size for C5 is at 188 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00156-1-c5-primary-antibodies-fcm-testing-2.pngAnti-C5 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-C5 antibody (A00156-1). Overlay histogram showing ANA-1 cells stained with A00156-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-C5 Antibody (A00156-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-camp-picoband-trade-antibody-a05475-1-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05475-1-camp-primary-antibodies-wb-testing-1.jpgAnti-Camp Antibody Picoband® Western blot analysis of Camp using anti-Camp antibody (A05475-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Camp antigen affinity purified polyclonal antibody (Catalog # A05475-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Camp at approximately 20 kDa. The expected band size for Camp is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05475-1-camp-primary-antibodies-wb-testing-2.pngAnti-Camp Antibody Picoband® Western blot analysis of Camp using anti-Camp antibody (A05475-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MADB106 cells under normal culture conditions, Lane 2:MADB106 cells treated with agonist, Lane 3:MADB106 cells treated with inhibitor. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Camp antigen affinity purified polyclonal antibody (Catalog # A05475-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Camp at approximately 17-20 kDa. The expected band size for Camp is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-caspase-3-p17-casp3-picoband-trade-antibody-a00334-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00334-2-casp3-primary-antibodies-wb-testing-1.jpgAnti-Caspase-3(p17)/CASP3 Antibody Picoband® Western blot analysis of Caspase-3(P17)/CASP3 using anti-Caspase-3(P17)/CASP3 antibody (A00334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3(P17)/CASP3 antigen affinity purified polyclonal antibody (Catalog # A00334-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3(P17)/CASP3 at approximately 32 kDa. The expected band size for Caspase-3(P17)/CASP3 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00334-2-casp3-primary-antibodies-if-testing-2.jpgAnti-Caspase-3(p17)/CASP3 Antibody Picoband® IF analysis of Caspase-3(P17)/CASP3 using anti-Caspase-3(P17)/CASP3 antibody (A00334-2). Caspase-3(P17)/CASP3 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Caspase-3(P17)/CASP3 Antibody (A00334-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00334-2-casp3-primary-antibodies-fcm-testing-3.pngAnti-Caspase-3(p17)/CASP3 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-Caspase-3(P17)/CASP3 antibody (A00334-2). Overlay histogram showing Caco-2 cells stained with A00334-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-3(P17)/CASP3 Antibody (A00334-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00334-2-fmars-05-00203-g002.jpgAnti-Caspase-3(p17)/CASP3 Antibody Picoband®Cv MC proliferation and apoptosis assays. The location of mantle tissue used for cell culture was indicated by dashed line (a) . Cv MCs after 48-h culture was shown (b) . Changes of medium pH in response to increased atmospheric CO 2 ( n = 4), (c) . Results of IFs with Ki-67 (d) and caspase 3 (e) were also quantified with the cells treated by ambient air and increased CO 2 concentration (2.5%). n = 8. Scale bar: 50 μm. Error bar: standard error of mean. Index in Frontiersin under a CC BY license. DOI: <a href='https://doi.org/10.3389/fmars.2018.00203'>10.3389/fmars.2018.00203</a> https://www.bosterbio.com/products/primary-antibodies/anti-caspase-8-casp8-picoband-trade-antibody-a00042-2-boster.html2026-03-17T05:12:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-2-casp8-primary-antibodies-wb-testing-1.jpgAnti-Caspase-8/CASP8 Antibody Picoband® Western blot analysis of Caspase-8/CASP8 using anti-Caspase-8/CASP8 antibody (A00042-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human RT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-8/CASP8 antigen affinity purified polyclonal antibody (Catalog # A00042-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-8/CASP8 at approximately 43 kDa. The expected band size for Caspase-8/CASP8 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-2-casp8-primary-antibodies-fcm-testing-2.pngAnti-Caspase-8/CASP8 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Caspase-8(P18)/CASP8 antibody (A00042-2). Overlay histogram showing U251 cells stained with A00042-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-8(P18)/CASP8 Antibody (A00042-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ccl21-picoband-trade-antibody-a00765-2-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00765-2-ccl21-primary-antibodies-wb-testing-1.jpgAnti-Ccl21 Antibody Picoband® Western blot analysis of Ccl21 using anti-Ccl21 antibody (A00765-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl21 antigen affinity purified polyclonal antibody (Catalog # A00765-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccl21 at approximately 15 kDa. The expected band size for Ccl21 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00765-2-ccl21-primary-antibodies-fcm-testing-2.pngAnti-Ccl21 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-Ccl21 antibody (A00765-2). Overlay histogram showing C6 cells stained with A00765-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Ccl21 Antibody (A00765-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd4-picoband-trade-antibody-a00344-3-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00344-3-cd4-primary-antibodies-ihc-testing-1.jpgAnti-Cd4 Antibody IHC analysis of Cd4 using anti-Cd4 antibody (A00344-3). Cd4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd4 Antibody (A00344-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cd9-picoband-trade-antibody-a01202-2-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01202-2-cd9-primary-antibodies-wb-testing-1.jpgAnti-CD9 Antibody Picoband® Western blot analysis of CD9 using anti-CD9 antibody (A01202-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # A01202-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 22 kDa. The expected band size for CD9 is at 25 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01202-2-10020_2025_1192_fig6_html.pngAnti-CD9 Antibody Picoband®Identification of hAMSC-Exos ( A ) The morphology of hAMSC-Exos was observed using transmission electron microscopy. Scale bar = 100 nm. ( B ) The hAMSC-Exos particle size distribution was detected using NTA. ( C ) Western blot was used to detect the expression of hAMSC-Exos surface proteins CD9, CD63 and CD81 Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01192-8'>40329179</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01202-2-cd9-primary-antibodies-ihc-testing-2.jpgAnti-CD9 Antibody Picoband® IHC analysis of CD9 using anti-CD9 antibody (A01202-2). CD9 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD9 Antibody (A01202-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01202-2-cd9-primary-antibodies-ihc-testing-3.jpgAnti-CD9 Antibody Picoband® IHC analysis of CD9 using anti-CD9 antibody (A01202-2). CD9 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD9 Antibody (A01202-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01202-2-cd9-primary-antibodies-if-testing-4.jpgAnti-CD9 Antibody Picoband® IF analysis of CD9 using anti-CD9 antibody (A01202-2). CD9 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD9 Antibody (A01202-2) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01202-2-cd9-primary-antibodies-fcm-testing-5.pngAnti-CD9 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-CD9 antibody (A01202-2). Overlay histogram showing JK cells stained with A01202-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD9 Antibody (A01202-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd14-picoband-trade-antibody-a00137-2-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00137-2-cd14-primary-antibodies-ihc-testing-1.jpgAnti-CD14 Antibody IHC analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00137-2-cd14-primary-antibodies-ihc-testing-2.jpgAnti-CD14 Antibody IHC analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00137-2-cd14-primary-antibodies-ihc-testing-3.jpgAnti-CD14 Antibody IHC analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in a paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00137-2-cd14-primary-antibodies-ihc-testing-4.jpgAnti-CD14 Antibody IHC analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00137-2-cd14-primary-antibodies-ihc-testing-5.jpgAnti-CD14 Antibody IHC analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00137-2-cd14-primary-antibodies-if-testing-6.jpgAnti-CD14 Antibody IF analysis of CD14 using anti-CD14 antibody (A00137-2). CD14 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CD14 Antibody (A00137-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00137-2-cd14-primary-antibodies-fcm-testing-7.pngAnti-CD14 Antibody Flow Cytometry analysis of Caco-2 cells using anti-CD14 antibody (A00137-2). Overlay histogram showing Caco-2 cells stained with A00137-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD14 Antibody (A00137-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd27-picoband-trade-antibody-a01148-2-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01148-2-cd27-primary-antibodies-ihc-testing-1.jpgAnti-Cd27 Antibody IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2). Cd27 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01148-2-41598_2025_88671_fig9_html.pngAnti-Cd27 AntibodyRelative expression of genes expression in GBM tissues. Expression levels of WTAP and ZC3H13 genes were compared with those of the control group on GBM tissues, 10 GBM and adjacent tissues, respectively ( A ). The expression levels of siRNA WTAP and overexpression ZC3H13 genes ( B and C ). The expression levels of CD27, CD70, CD80, CD86, ICOS, CTLA4, and LAG3 in siRNA WTAP and overexpression ZC3H13 were demonstrated in Fig. D and F, respectively. The result were determined by qRT-PCR. The data are mean ± standard deviation of six replicate experiments. * P < 0.05, **P < 0.01 and ***P < 0.001 compared with the corresponding control values. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-88671-4'>39910141</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01148-2-cd27-primary-antibodies-ihc-testing-2.jpgAnti-Cd27 Antibody IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2). Cd27 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01148-2-cd27-primary-antibodies-ihc-testing-3.jpgAnti-Cd27 Antibody IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2). Cd27 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01148-2-cd27-primary-antibodies-fcm-testing-4.pngAnti-Cd27 Antibody Flow Cytometry analysis of mouse PBMC cells using anti-Cd27 antibody (A01148-2). Overlay histogram showing mouse PBMC cells stained with A01148-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cd27 Antibody (A01148-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd80-picoband-trade-antibody-a00196-3-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00196-3-cd80-primary-antibodies-wb-testing-1.jpgAnti-Cd80 Antibody Picoband® Western blot analysis of Cd80 using anti-Cd80 antibody (A00196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd80 antigen affinity purified polyclonal antibody (Catalog # A00196-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd80 at approximately 70 kDa. The expected band size for Cd80 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00196-3-41598_2025_88671_fig9_html.pngAnti-Cd80 Antibody Picoband®Relative expression of genes expression in GBM tissues. Expression levels of WTAP and ZC3H13 genes were compared with those of the control group on GBM tissues, 10 GBM and adjacent tissues, respectively ( A ). The expression levels of siRNA WTAP and overexpression ZC3H13 genes ( B and C ). The expression levels of CD27, CD70, CD80, CD86, ICOS, CTLA4, and LAG3 in siRNA WTAP and overexpression ZC3H13 were demonstrated in Fig. D and F, respectively. The result were determined by qRT-PCR. The data are mean ± standard deviation of six replicate experiments. * P < 0.05, **P < 0.01 and ***P < 0.001 compared with the corresponding control values. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-88671-4'>39910141</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00196-3-cd80-primary-antibodies-fcm-testing-2.pngAnti-Cd80 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Cd80 antibody (A00196-3). Overlay histogram showing ANA-1 cells stained with A00196-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cd80 Antibody (A00196-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd86-picoband-trade-antibody-a00220-3-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-3-cd86-primary-antibodies-wb-testing-1.jpgAnti-CD86 Antibody Picoband® Western blot analysis of CD86 using anti-CD86 antibody (A00220-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD86 antigen affinity purified polyclonal antibody (Catalog # A00220-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD86 at approximately 60-80 kDa. The expected band size for CD86 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-3-cd86-primary-antibodies-if-testing-2.jpgAnti-CD86 Antibody Picoband® IF analysis of CD86 using anti-CD86 antibody (A00220-3). CD86 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CD86 Antibody (A00220-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-3-cd86-primary-antibodies-fcm-testing-3.pngAnti-CD86 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-CD86 antibody (A00220-3). Overlay histogram showing Daudi cells stained with A00220-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD86 Antibody (A00220-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd86-picoband-trade-antibody-a00220-4-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-4-fphar-16-1430536-g006.jpgAnti-Cd86 Antibody Picoband®YNJ reduces the secretion of inflammatory factors and promotes M2 macrophage polarization. (A–C) ELISA of TNF-α (A) , IL-6 (B) , and IL-1β (C) contents in the supernatant of cultured RAW264.7 macrophages. (D) IF images of CD86 and CD206 detection in cultured RAW264.7 macrophages. (E, F) Statistical analysis of CD86 and CD206 expression by IF. The data are presented as the mean ± SD (n = 6). ** P < 0.01. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1430536/full'>39925847</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-4-cd86-primary-antibodies-wb-testing-1.jpgAnti-Cd86 Antibody Picoband® Western blot analysis of Cd86 using anti-Cd86 antibody (A00220-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd86 antigen affinity purified polyclonal antibody (Catalog # A00220-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd86 at approximately 60-80 kDa. The expected band size for Cd86 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-4-cd86-primary-antibodies-ihc-testing-2.jpgAnti-Cd86 Antibody Picoband® IHC analysis of Cd86 using anti-Cd86 antibody (A00220-4). Cd86 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd86 Antibody (A00220-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-4-cd86-primary-antibodies-if-testing-1.pngAnti-Cd86 Antibody Picoband®IF analysis of Cd86 using anti-Cd86 antibody (A00220-4). Cd86 was detected in a paraffin-embedded section of human normal and preterm placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1;200 rabbit anti-Cd86 Antibody (A00220-4) overnight at 4°C. DyLight 594 Goat Anti-Rabbit IgG (H+L) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a Leica DM2500 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00220-4-cd86-primary-antibodies-fcm-testing-3_1.pngAnti-Cd86 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Cd86 antibody (A00220-4). Overlay histogram showing ANA-1 cells stained with A00220-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cd86 Antibody (A00220-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd226-picoband-trade-antibody-a01094-3-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01094-3-cd226-primary-antibodies-wb-testing-1.jpgAnti-CD226 Antibody Picoband® Western blot analysis of CD226 using anti-CD226 antibody (A01094-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD226 antigen affinity purified polyclonal antibody (Catalog # A01094-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD226 at approximately 70 kDa. The expected band size for CD226 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01094-3-cd226-primary-antibodies-fcm-testing-2.pngAnti-CD226 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-CD226 antibody (A01094-3). Overlay histogram showing Jurkat cells stained with A01094-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD226 Antibody (A01094-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-m-cadherin-cdh15-picoband-trade-antibody-a10888-1-boster.html2026-04-01T05:01:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10888-1-cdh15-primary-antibodies-wb-testing-1.jpgAnti-M Cadherin/CDH15 Antibody Picoband® Western blot analysis of M Cadherin/CDH15 using anti-M Cadherin/CDH15 antibody (A10888-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-M Cadherin/CDH15 antigen affinity purified polyclonal antibody (Catalog # A10888-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for M Cadherin/CDH15 at approximately 130 kDa. The expected band size for M Cadherin/CDH15 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10888-1-cdh15-primary-antibodies-ihc-testing-4.jpgAnti-M Cadherin/CDH15 Antibody Picoband®IHC analysis of Cadherin-15/CDH15 using anti-Cadherin-15/CDH15 antibody (A10888-1). Cadherin-15/CDH15 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cadherin-15/CDH15 Antibody (A10888-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10888-1-cdh15-primary-antibodies-ihc-testing-2.jpgAnti-M Cadherin/CDH15 Antibody Picoband® IHC analysis of M Cadherin/CDH15 using anti-M Cadherin/CDH15 antibody (A10888-1). M Cadherin/CDH15 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-M Cadherin/CDH15 Antibody (A10888-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10888-1-cdh15-primary-antibodies-ihc-testing-3.jpgAnti-M Cadherin/CDH15 Antibody Picoband® IHC analysis of M Cadherin/CDH15 using anti-M Cadherin/CDH15 antibody (A10888-1). M Cadherin/CDH15 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-M Cadherin/CDH15 Antibody (A10888-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10888-1-cdh15-primary-antibodies-fcm-testing-4.pngAnti-M Cadherin/CDH15 Antibody Picoband® Flow Cytometry analysis of C2C12 cells using anti-M Cadherin/CDH15 antibody (A10888-1). Overlay histogram showing C2C12 cells stained with A10888-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-M Cadherin/CDH15 Antibody (A10888-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-p19-ink4d-cdkn2d-picoband-trade-antibody-a04390-2-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04390-2-cdkn2d-primary-antibodies-wb-testing-1.jpgAnti-p19 INK4d/CDKN2D Antibody Picoband® Western blot analysis of P19 INK4d/CDKN2D using anti-P19 INK4d/CDKN2D antibody (A04390-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P19 INK4d/CDKN2D antigen affinity purified polyclonal antibody (Catalog # A04390-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P19 INK4d/CDKN2D at approximately 18 kDa. The expected band size for P19 INK4d/CDKN2D is at 18 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04390-2-cdkn2d-primary-antibodies-fcm-testing-2.pngAnti-p19 INK4d/CDKN2D Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-P19 INK4d/CDKN2D antibody (A04390-2). Overlay histogram showing U87 cells stained with A04390-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P19 INK4d/CDKN2D Antibody (A04390-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-amcase-chia-picoband-trade-antibody-a00437-1-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00437-1-chia-primary-antibodies-wb-testing-1_1.jpgAnti-AMCase/CHIA Antibody Picoband®Western blot analysis of AMCase/CHIA using anti-AMCase/CHIA antibody (A00437-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMCase/CHIA antigen affinity purified polyclonal antibody (A00437-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AMCase/CHIA at approximately 52 kDa. The expected band size for AMCase/CHIA is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00437-1-chia-primary-antibodies-ihc-testing-1.jpgAnti-AMCase/CHIA Antibody Picoband®IHC analysis of AMCase/CHIA using anti-AMCase/CHIA antibody (A00437-1). AMCase/CHIA was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AMCase/CHIA Antibody (A00437-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ciita-picoband-trade-antibody-a01556-3-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01556-3-ciita-primary-antibodies-wb-testing-1.jpgAnti-CIITA Antibody Picoband® Western blot analysis of CIITA using anti-CIITA antibody (A01556-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIITA antigen affinity purified polyclonal antibody (Catalog # A01556-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIITA at approximately 123 kDa. The expected band size for CIITA is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01556-3-ciita-primary-antibodies-fcm-testing-2.pngAnti-CIITA Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-CIITA antibody (A01556-3). Overlay histogram showing Raji cells stained with A01556-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIITA Antibody (A01556-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-claudin18-cldn18-picoband-trade-antibody-a09129-1-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09129-1-cldn18-primary-antibodies-wb-testing-1.jpgAnti-Claudin18/CLDN18 Antibody Picoband® Western blot analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin18/CLDN18 antigen affinity purified polyclonal antibody (Catalog # A09129-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin18/CLDN18 at approximately 30 kDa. The expected band size for Claudin18/CLDN18 is at 30 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09129-1-cldn18-primary-antibodies-ihc-testing-2.jpgAnti-Claudin18/CLDN18 Antibody Picoband® IHC analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Claudin18/CLDN18 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Claudin18/CLDN18 Antibody (A09129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09129-1-cldn18-primary-antibodies-ihc-testing-3.jpgAnti-Claudin18/CLDN18 Antibody Picoband® IHC analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Claudin18/CLDN18 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Claudin18/CLDN18 Antibody (A09129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09129-1-cldn18-primary-antibodies-if-testing-4.jpgAnti-Claudin18/CLDN18 Antibody Picoband® IF analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Claudin18/CLDN18 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Claudin18/CLDN18 Antibody (A09129-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09129-1-cldn18-primary-antibodies-fcm-testing-5_1.pngAnti-Claudin18/CLDN18 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-Claudin18/CLDN18 antibody (A09129-1). Overlay histogram showing Hela cells stained with A09129-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Claudin18/CLDN18 Antibody (A09129-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09129-1-cldn18-primary-antibodies-if-testing-6.jpgAnti-Claudin18/CLDN18 Antibody Picoband® IF analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Claudin18/CLDN18 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Claudin18/CLDN18 Antibody (A09129-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09129-1-cldn18-primary-antibodies-if-testing-7.jpgAnti-Claudin18/CLDN18 Antibody Picoband® IF analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Claudin18/CLDN18 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Claudin18/CLDN18 Antibody (A09129-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-calmegin-clgn-picoband-trade-antibody-a05261-1-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05261-1-clgn-primary-antibodies-wb-testing-1.jpgAnti-Calmegin/CLGN Antibody Picoband® Western blot analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calmegin/CLGN antigen affinity purified polyclonal antibody (Catalog # A05261-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calmegin/CLGN at approximately 93 kDa. The expected band size for Calmegin/CLGN is at 93 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05261-1-clgn-primary-antibodies-ihc-testing-2.jpgAnti-Calmegin/CLGN Antibody Picoband® IHC analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-1). Calmegin/CLGN was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calmegin/CLGN Antibody (A05261-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05261-1-clgn-primary-antibodies-ihc-testing-3.jpgAnti-Calmegin/CLGN Antibody Picoband® IHC analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-1). Calmegin/CLGN was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calmegin/CLGN Antibody (A05261-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05261-1-clgn-primary-antibodies-ihc-testing-4.jpgAnti-Calmegin/CLGN Antibody Picoband® IHC analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-1). Calmegin/CLGN was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calmegin/CLGN Antibody (A05261-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05261-1-clgn-primary-antibodies-ihc-testing-5.jpgAnti-Calmegin/CLGN Antibody Picoband® IHC analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-1). Calmegin/CLGN was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calmegin/CLGN Antibody (A05261-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05261-1-clgn-primary-antibodies-fcm-testing-6.pngAnti-Calmegin/CLGN Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-Calmegin/CLGN antibody (A05261-1). Overlay histogram showing Jurkat cells stained with A05261-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calmegin/CLGN Antibody (A05261-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dectin-1-clec7a-picoband-trade-antibody-a02731-1-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02731-1-clec7a-primary-antibodies-wb-testing-1.jpgAnti-Dectin-1/Clec7a Antibody Picoband® Western blot analysis of Dectin-1/Clec7a using anti-Dectin-1/Clec7a antibody (A02731-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dectin-1/Clec7a antigen affinity purified polyclonal antibody (Catalog # A02731-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dectin-1/Clec7a at approximately 33 kDa. The expected band size for Dectin-1/Clec7a is at 33 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02731-1-clec7a-primary-antibodies-ihc-testing-2.jpgAnti-Dectin-1/Clec7a Antibody Picoband® IHC analysis of Dectin-1/Clec7a using anti-Dectin-1/Clec7a antibody (A02731-1). Dectin-1/Clec7a was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dectin-1/Clec7a Antibody (A02731-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02731-1-clec7a-primary-antibodies-ihc-testing-3.jpgAnti-Dectin-1/Clec7a Antibody Picoband® IHC analysis of Dectin-1/Clec7a using anti-Dectin-1/Clec7a antibody (A02731-1). Dectin-1/Clec7a was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dectin-1/Clec7a Antibody (A02731-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02731-1-clec7a-primary-antibodies-fcm-testing-4.pngAnti-Dectin-1/Clec7a Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Dectin-1/Clec7a antibody (A02731-1). Overlay histogram showing ANA-1 cells stained with A02731-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Dectin-1/Clec7a Antibody (A02731-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-calmegin-clgn-picoband-trade-antibody-a05261-2-boster.html2026-03-17T05:12:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05261-2-clgn-primary-antibodies-wb-testing-1.jpgAnti-Calmegin/Clgn Antibody Picoband® Western blot analysis of Calmegin/Clgn using anti-Calmegin/Clgn antibody (A05261-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat H9C2(2-1) whole cell lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calmegin/Clgn antigen affinity purified polyclonal antibody (Catalog # A05261-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calmegin/Clgn at approximately 95 kDa. The expected band size for Calmegin/Clgn is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cpeb4-picoband-trade-antibody-a06139-2-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06139-2-cpeb4-primary-antibodies-wb-testing-1.jpgAnti-CPEB4 Antibody Picoband® Western blot analysis of CPEB4 using anti-CPEB4 antibody (A06139-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPEB4 antigen affinity purified polyclonal antibody (Catalog # A06139-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPEB4 at approximately 90 kDa. The expected band size for CPEB4 is at 80 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06139-2-cpeb4-primary-antibodies-fcm-testing-2.pngAnti-CPEB4 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-CPEB4 antibody (A06139-2). Overlay histogram showing PC-3 cells stained with A06139-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPEB4 Antibody (A06139-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06139-2-cpeb4-primary-antibodies-fcm-testing-3.pngAnti-CPEB4 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CPEB4 antibody (A06139-2). Overlay histogram showing SiHa cells stained with A06139-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPEB4 Antibody (A06139-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cpo-picoband-trade-antibody-a03649-1-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03649-1-cpo-primary-antibodies-wb-testing-1.jpgAnti-CPO Antibody Picoband® Western blot analysis of CPO using anti-CPO antibody (A03649-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human AGS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPO antigen affinity purified polyclonal antibody (Catalog # A03649-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPO at approximately 57 kDa. The expected band size for CPO is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03649-1-cpo-primary-antibodies-fcm-testing-2.pngAnti-CPO Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CPO antibody (A03649-1). Overlay histogram showing SiHa cells stained with A03649-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPO Antibody (A03649-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cpt1a-picoband-trade-antibody-a00917-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00917-3-cpt1a-primary-antibodies-wb-testing-1.jpgAnti-CPT1A Antibody Picoband® Western blot analysis of CPT1A using anti-CPT1A antibody (A00917-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT-4 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1A antigen affinity purified polyclonal antibody (Catalog # A00917-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1A at approximately 88 kDa. The expected band size for CPT1A is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00917-3-cpt1a-primary-antibodies-ihc-testing-2.jpgAnti-CPT1A Antibody Picoband® IHC analysis of CPT1A using anti-CPT1A antibody (A00917-3). CPT1A was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPT1A Antibody (A00917-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00917-3-cpt1a-primary-antibodies-ihc-testing-3.jpgAnti-CPT1A Antibody Picoband® IHC analysis of CPT1A using anti-CPT1A antibody (A00917-3). CPT1A was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPT1A Antibody (A00917-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00917-3-cpt1a-primary-antibodies-ihc-testing-4.jpgAnti-CPT1A Antibody Picoband® IHC analysis of CPT1A using anti-CPT1A antibody (A00917-3). CPT1A was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPT1A Antibody (A00917-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00917-3-cpt1a-primary-antibodies-ihc-testing-5.jpgAnti-CPT1A Antibody Picoband® IHC analysis of CPT1A using anti-CPT1A antibody (A00917-3). CPT1A was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPT1A Antibody (A00917-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00917-3-cpt1a-primary-antibodies-if-testing-6.jpgAnti-CPT1A Antibody Picoband® IF analysis of CPT1A using anti-CPT1A antibody (A00917-3). CPT1A was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CPT1A Antibody (A00917-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00917-3-cpt1a-primary-antibodies-fcm-testing-7.pngAnti-CPT1A Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-CPT1A antibody (A00917-3). Overlay histogram showing U251 cells stained with A00917-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPT1A Antibody (A00917-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-delta-1-catenin-cas-ctnnd1-picoband-trade-antibody-a02333-4-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02333-4-ctnnd1-primary-antibodies-wb-testing-1.jpgAnti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband® Western blot analysis of Delta 1 Catenin/CAS/CTNND1 using anti-Delta 1 Catenin/CAS/CTNND1 antibody (A02333-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Delta 1 Catenin/CAS/CTNND1 antigen affinity purified polyclonal antibody (Catalog # A02333-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Delta 1 Catenin/CAS/CTNND1 at approximately 100-110 kDa. The expected band size for Delta 1 Catenin/CAS/CTNND1 is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02333-4-ctnnd1-primary-antibodies-fcm-testing-2.pngAnti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-Delta 1 Catenin/CAS/CTNND1 antibody (A02333-4). Overlay histogram showing Caco-2 cells stained with A02333-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Delta 1 Catenin/CAS/CTNND1 Antibody (A02333-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cathepsin-b-ctsb-picoband-trade-antibody-a01456-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01456-3-ctsb-primary-antibodies-wb-testing-1_1.jpgAnti-Cathepsin B/CTSB Antibody Picoband® Western blot analysis of Cathepsin B/CTSB using anti-Cathepsin B/CTSB antibody (A01456-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mosue liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin B/CTSB antigen affinity purified polyclonal antibody (Catalog # A01456-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin B/CTSB at approximately 23,31 kDa. The expected band size for Cathepsin B/CTSB is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01456-3-ctsb-primary-antibodies-ihc-testing-2.jpgAnti-Cathepsin B/CTSB Antibody Picoband® IHC analysis of Cathepsin B/CTSB using anti-Cathepsin B/CTSB antibody (A01456-3). Cathepsin B/CTSB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin B/CTSB Antibody (A01456-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01456-3-ctsb-primary-antibodies-ihc-testing-3.jpgAnti-Cathepsin B/CTSB Antibody Picoband® IHC analysis of Cathepsin B/CTSB using anti-Cathepsin B/CTSB antibody (A01456-3). Cathepsin B/CTSB was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin B/CTSB Antibody (A01456-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01456-3-ctsb-primary-antibodies-ihc-testing-4.jpgAnti-Cathepsin B/CTSB Antibody Picoband® IHC analysis of Cathepsin B/CTSB using anti-Cathepsin B/CTSB antibody (A01456-3). Cathepsin B/CTSB was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin B/CTSB Antibody (A01456-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01456-3-ctsb-primary-antibodies-fcm-testing-7.jpgAnti-Cathepsin B/CTSB Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Cathepsin B/CTSB antibody (A01456-3). Overlay histogram showing SiHa cells stained with A01456-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cathepsin B/CTSB Antibody (A01456-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01456-3-ctsb-primary-antibodies-ihc-testing-5.jpgAnti-Cathepsin B/CTSB Antibody Picoband® IHC analysis of Cathepsin B/CTSB using anti-Cathepsin B/CTSB antibody (A01456-3). Cathepsin B/CTSB was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin B/CTSB Antibody (A01456-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01456-3-ctsb-primary-antibodies-ihc-testing-6.jpgAnti-Cathepsin B/CTSB Antibody Picoband® IHC analysis of Cathepsin B/CTSB using anti-Cathepsin B/CTSB antibody (A01456-3). Cathepsin B/CTSB was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin B/CTSB Antibody (A01456-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cathepsin-l-mep-ctsl-picoband-trade-antibody-a01589-2-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01589-2-ctsl-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin L/MEP/CTSL Antibody Picoband® Western blot analysis of Cathepsin L/MEP/CTSL using anti-Cathepsin L/MEP/CTSL antibody (A01589-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin L/MEP/CTSL antigen affinity purified polyclonal antibody (Catalog # A01589-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin L/MEP/CTSL at approximately 37 kDa. The expected band size for Cathepsin L/MEP/CTSL is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cathepsin-l-mep-ctsl-picoband-trade-antibody-a01589-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01589-3-ctsl-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin L/MEP/Ctsl Antibody Picoband® Western blot analysis of Cathepsin L/MEP/Ctsl using anti-Cathepsin L/MEP/Ctsl antibody (A01589-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin L/MEP/Ctsl antigen affinity purified polyclonal antibody (Catalog # A01589-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin L/MEP/Ctsl at approximately 20-42 kDa. The expected band size for Cathepsin L/MEP/Ctsl is at 38 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01589-3-ctsl-primary-antibodies-ihc-testing-2.jpgAnti-Cathepsin L/MEP/Ctsl Antibody Picoband® IHC analysis of Cathepsin L/MEP/Ctsl using anti-Cathepsin L/MEP/Ctsl antibody (A01589-3). Cathepsin L/MEP/Ctsl was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin L/MEP/Ctsl Antibody (A01589-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01589-3-ctsl-primary-antibodies-fcm-testing-3.pngAnti-Cathepsin L/MEP/Ctsl Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Cathepsin L/MEP/Ctsl antibody (A01589-3). Overlay histogram showing HEPA1-6 cells stained with A01589-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cathepsin L/MEP/Ctsl Antibody (A01589-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cxcr2-picoband-trade-antibody-a00455-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00455-3-cxcr2-primary-antibodies-wb-testing-1.jpgAnti-CXCR2 Antibody Picoband® Western blot analysis of CXCR2 using anti-CXCR2 antibody (A00455-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR2 antigen affinity purified polyclonal antibody (Catalog # A00455-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR2 at approximately 41-50 kDa. The expected band size for CXCR2 is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00455-3-fphar-10-00307-g001.jpgAnti-CXCR2 Antibody Picoband®Expressions of CXCL6, CXCR1, and CXCR2 in osteosarcoma (OS) cells. The mRNA expressions of CXCL6 (A) , CXCR1 (B) , and CXCR2 (C) in multiple OS cell lines were evaluated by real-time PCR. The protein levels of CXCL6 (D) , CXCR1 (E) , and CXCR2 (F) in multiple OS cell lines were detected by western blot assay. (G–I) The protein quantification histograms were shown. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00307/full'>30984000</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00455-3-fphar-10-00307-g004.jpgAnti-CXCR2 Antibody Picoband®CXCL6/CXCR2 axis contributed to migration and invasion of OS cells. After transfection with siRNAs for 24 h, OS cells were treated with 100 ng/ml rhCXCL6 for 24 h. The mRNA expression of CXCR2 in SaOS-2 (A) and U2OS (B) cells was detected by real-time PCR. The protein expression of CXCR2 in SaOS-2 (C) and U2OS (D) cells was assessed by western blot assay. The protein quantification histograms were shown. (E) The migration of SaOS-2 and U2OS cells was detected by Transwell assay (no matrigel). Scal bar = 100 μm. (F,G) The number of migrated cells was shown. (H) The invasion of SaOS-2 and U2OS cells was determined by Transwell assay (matrigel). Scal bar = 100 μm. (I,J) The number of invasive cells was shown. (K) The protein levels of MMP9 and Snail in SaOS-2 and U2OS cells were detected by western blot assay. (L–O) The protein quantification histograms were shown. (P) MMP-9 activity in the supernatant fluid of cultured SaOS-2 and U2OS cells was determined by gelatin zymography method. (Q,R) The quantification histograms were shown. ∗∗ P < 0.01, ∗∗∗ P < 0.001, versus the SaOS-2+NC or U2OS+NC group. # P < 0.05, ## P < 0.01, ### P < 0.001, versus the rhCXCL6+NC group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00307/full'>30984000</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00455-3-fphar-10-00307-g005.jpgAnti-CXCR2 Antibody Picoband®Effect of CXCL6/CXCR2 axis on E-cadherin and N-cadherin expressions. The expressions of E-cadherin (A) and N-cadherin (B) in SaOS-2 and U2OS cells with different treatments were determined by immunofluorescence assay. Scal bar = 50 μm. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00307/full'>30984000</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00455-3-fphar-10-00307-g006.jpgAnti-CXCR2 Antibody Picoband®PI3K/AKT and β-catenin signaling pathways participated in the regulation of migration, invasion and EMT by CXCL6/CXCR2 axis in OS cells. After transfection with siRNAs for 24 h, OS cells were treated with 100 ng/ml rhCXCL6 for 24 h. (A) The protein levels of p-AKT, AKT, and nuclear β-catenin in SaOS-2 and U2OS cells were detected by western blot assay. β-actin and Histone H3 were used as loading controls. (B–E) The protein quantification histograms were shown. OS cells were pre-treated with 50 μM LY294002 or 10 μM XAV939 for 1 h, then treated with 100 ng/ml rhCXCL6 for 24 h. (F) The migration of SaOS-2 and U2OS cells was detected by Transwell assay (no matrigel). Scal bar = 100 μm. (G,H) The number of migrated cells was shown. (I) The invasion of SaOS-2 and U2OS cells was determined by Transwell assay (matrigel). Scal bar = 100 μm. (J,K) The number of invasive cells was shown. (L) The protein levels of E-cadherin, N-cadherin, Snail, and MMP9 in SaOS-2 and U2OS cells were detected by western blot assay. (M–T) The protein quantification histograms were shown. (U) MMP-9 activity in the supernatant fluid of cultured SaOS-2 and U2OS cells was assessed by gelatin zymography assay. (V,W) The quantification histograms were shown. ∗∗∗ P < 0.001, versus the OS cell or OS cell +NC group. # P < 0.05, ## P < 0.01, ### P < 0.001, versus the rhCXCL6+NC or rhCXCL6 group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00307/full'>30984000</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00455-3-fphar-10-00307-g007.jpgAnti-CXCR2 Antibody Picoband®Effect of CXCL6/CXCR2 axis on OS tumor growth and pulmonary metastasis in vivo . U2OS cells were infected with lentivirus expressing CXCL6 or NC. The mRNA (A) and protein level (B) of CXCL6 in U2OS cells were determined by real-time PCR and western blot assay. (C) After the injection of LV-CXCL6 or LV-NC U2OS cells for 21 days, the representative images of nude mice and their removed xenograft tumors were shown. (D) The tumor volume was calculated and the tumor growth-curve was shown. (E) The weight of xenograft tumors was shown. (F) The expression of PCNA in tumor tissues was detected by immunohistochemical staining. Scal bar = 50 μm. (G) The percentage of PCNA positive cells were calculated and shown. (H) The protein levels of p-AKT, AKT, and nuclear β-catenin in tumor tissues were detected by western blot assay. β-actin and Histone H3 were used as loading controls. (I,J) The protein quantification histograms were shown. (K) Representative images of the lung of nude mice. (L) The number of lung metastatic nodes was quantified. ∗∗∗ P < 0.001, versus the LV-NC group. ## P < 0.01, ### P < 0.001, versus the LV-CXCL6 group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00307/full'>30984000</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00455-3-cxcr2-primary-antibodies-fcm-testing-2.pngAnti-CXCR2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-CXCR2 antibody (A00455-3). Overlay histogram showing THP-1 cells stained with A00455-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (A00455-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cytochrome-p450-3a4-cyp3a4-picoband-trade-antibody-a00339-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00339-3-cyp3a4-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband® Western blot analysis of Cytochrome P450 3A4/CYP3A4 using anti-Cytochrome P450 3A4/CYP3A4 antibody (A00339-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome P450 3A4/CYP3A4 antigen affinity purified polyclonal antibody (Catalog # A00339-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome P450 3A4/CYP3A4 at approximately 50 kDa. The expected band size for Cytochrome P450 3A4/CYP3A4 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00339-3-cyp3a4-primary-antibodies-fcm-testing-2.pngAnti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Cytochrome P450 3A4/CYP3A4 antibody (A00339-3). Overlay histogram showing HepG2 cells stained with A00339-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytochrome P450 3A4/CYP3A4 Antibody (A00339-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aromatase-cyp19a1-picoband-trade-antibody-a00071-2-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-2-cyp19a1-primary-antibodies-wb-testing-1.jpgAnti-Aromatase/Cyp19a1 Antibody Picoband® Western blot analysis of Aromatase/Cyp19a1 using anti-Aromatase/Cyp19a1 antibody (A00071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human HCCP tissue lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human SK-OV-3 whole cell lysates, Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase/Cyp19a1 antigen affinity purified polyclonal antibody (Catalog # A00071-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase/Cyp19a1 at approximately 49-58 kDa. The expected band size for Aromatase/Cyp19a1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-2-12958_2004_article_214_fig4_html.jpgAnti-Aromatase/Cyp19a1 Antibody Picoband®Western blot analysis of liver P450arom expressions of the rats. The upper picture shows the Western blot analysis of the liver aromatase P450. Densitometric analysis of the protein concentration using aromatase/β-actin expressed as the mean with SEM bar in each column indicated in the lower panel. * p < 0.05 vs corresponding intact controls, # p < 0.05 vs OVX1M. Index in PubMed under a CC BY license. PMID: <a href='https://rbej.biomedcentral.com/articles/10.1186/1477-7827-3-6'>15661083</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-2-12958_2004_article_214_fig5_html.jpgAnti-Aromatase/Cyp19a1 Antibody Picoband®Western blot analysis of SA adipose P450arom expressions of the rats. The upper picture shows the Western blot analysis of the SA adipose aromatase P450. Densitometric analysis of the protein concentration using aromatase/β-actin expressed as the mean with SEM bar in each column indicated in the lower panel. * p < 0.05 vs corresponding intact controls, # p < 0.05 vs OVX1M. Index in PubMed under a CC BY license. PMID: <a href='https://rbej.biomedcentral.com/articles/10.1186/1477-7827-3-6'>15661083</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00071-2-cyp19a1-primary-antibodies-wb-testing-2.jpgAnti-Aromatase/Cyp19a1 Antibody Picoband® Western blot analysis of Aromatase/Cyp19a1 using anti-Aromatase/Cyp19a1 antibody (A00071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aromatase/Cyp19a1 antigen affinity purified polyclonal antibody (Catalog # A00071-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aromatase/Cyp19a1 at approximately 49-58 kDa. The expected band size for Aromatase/Cyp19a1 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-dmc1-picoband-trade-antibody-a02978-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02978-3-dmc1-primary-antibodies-wb-testing-1.jpgAnti-DMC1 Antibody Picoband® Western blot analysis of DMC1 using anti-DMC1 antibody (A02978-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMC1 antigen affinity purified polyclonal antibody (Catalog # A02978-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DMC1 at approximately 37 kDa. The expected band size for DMC1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02978-3-dmc1-primary-antibodies-ihc-testing-2.jpgAnti-DMC1 Antibody Picoband® IHC analysis of DMC1 using anti-DMC1 antibody (A02978-3). DMC1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMC1 Antibody (A02978-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02978-3-dmc1-primary-antibodies-ihc-testing-3.jpgAnti-DMC1 Antibody Picoband® IHC analysis of DMC1 using anti-DMC1 antibody (A02978-3). DMC1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMC1 Antibody (A02978-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02978-3-dmc1-primary-antibodies-fcm-testing-4.pngAnti-DMC1 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-DMC1 antibody (A02978-3). Overlay histogram showing Jurkat cells stained with A02978-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DMC1 Antibody (A02978-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dnmt3b-picoband-trade-antibody-a00319-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00319-3-dnmt3b-primary-antibodies-wb-testing-1.jpgAnti-DNMT3B Antibody Picoband® Western blot analysis of DNMT3B using anti-DNMT3B antibody (A00319-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B antigen affinity purified polyclonal antibody (Catalog # A00319-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3B at approximately 96 kDa. The expected band size for DNMT3B is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00319-3-dnmt3b-primary-antibodies-fcm-testing-2.pngAnti-DNMT3B Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-DNMT3B antibody (A00319-3). Overlay histogram showing Caco-2 cells stained with A00319-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT3B Antibody (A00319-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ern2-picoband-trade-antibody-a05659-2-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05659-2-ern2-primary-antibodies-wb-testing-1.jpgAnti-ERN2 Antibody Picoband® Western blot analysis of ERN2 using anti-ERN2 antibody (A05659-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERN2 antigen affinity purified polyclonal antibody (Catalog # A05659-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERN2 at approximately 102 kDa. The expected band size for ERN2 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05659-2-ern2-primary-antibodies-fcm-testing-2.pngAnti-ERN2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-ERN2 antibody (A05659-2). Overlay histogram showing Jurkat cells stained with A05659-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERN2 Antibody (A05659-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fgf2-picoband-trade-antibody-a00121-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-3-fgf2-primary-antibodies-wb-testing-1.jpgAnti-FGF2 Antibody Picoband® Western blot analysis of FGF2 using anti-FGF2 antibody (A00121-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # A00121-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 18 kDa. The expected band size for FGF2 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-3-fgf2-primary-antibodies-ihc-testing-2.jpgAnti-FGF2 Antibody Picoband® IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3). FGF2 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-3-fgf2-primary-antibodies-ihc-testing-3.jpgAnti-FGF2 Antibody Picoband® IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3). FGF2 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-3-fgf2-primary-antibodies-ihc-testing-4.jpgAnti-FGF2 Antibody Picoband® IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3). FGF2 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-3-fgf2-primary-antibodies-ihc-testing-5.jpgAnti-FGF2 Antibody Picoband®IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3). FGF2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00121-3-fgf2-primary-antibodies-if-testing-5.jpgAnti-FGF2 Antibody Picoband® IF analysis of FGF2 using anti-FGF2 antibody (A00121-3). FGF2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FGF2 Antibody (A00121-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fgf11-picoband-trade-antibody-a13819-2-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13819-2-fgf11-primary-antibodies-wb-testing-1.jpgAnti-FGF11 Antibody Picoband® Western blot analysis of FGF11 using anti-FGF11 antibody (A13819-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF11 antigen affinity purified polyclonal antibody (Catalog # A13819-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF11 at approximately 25 kDa. The expected band size for FGF11 is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-foxl2-picoband-trade-antibody-a01185-1-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01185-1-foxl2-primary-antibodies-wb-testing-1.jpgAnti-FOXL2 Antibody Picoband® Western blot analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXL2 antigen affinity purified polyclonal antibody (Catalog # A01185-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXL2 at approximately 50 kDa. The expected band size for FOXL2 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01185-1-foxl2-primary-antibodies-ihc-testing-1.jpgAnti-FOXL2 Antibody Picoband®IHC analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). FOXL2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXL2 Antibody (A01185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01185-1-foxl2-primary-antibodies-ihc-testing-4.jpgAnti-FOXL2 Antibody Picoband®IHC analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). FOXL2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXL2 Antibody (A01185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01185-1-foxl2-primary-antibodies-ihc-testing-2.jpgAnti-FOXL2 Antibody Picoband® IHC analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). FOXL2 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXL2 Antibody (A01185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01185-1-foxl2-primary-antibodies-ihc-testing-3.jpgAnti-FOXL2 Antibody Picoband® IHC analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). FOXL2 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXL2 Antibody (A01185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01185-1-foxl2-primary-antibodies-fcm-testing-4.pngAnti-FOXL2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-FOXL2 antibody (A01185-1). Overlay histogram showing THP-1 cells stained with A01185-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXL2 Antibody (A01185-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-foxp3-picoband-trade-antibody-a00011-2-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00011-2-foxp3-primary-antibodies-wb-testing-1.jpgAnti-Foxp3 Antibody Picoband® Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tls-fus-picoband-trade-antibody-a00771-3-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00771-3-fus-primary-antibodies-wb-testing-1.jpgAnti-TLS/FUS Antibody Picoband® Western blot analysis of TLS/FUS using anti-TLS/FUS antibody (A00771-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human SH-SY5Y whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLS/FUS antigen affinity purified polyclonal antibody (Catalog # A00771-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLS/FUS at approximately 70 kDa. The expected band size for TLS/FUS is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00771-3-fus-primary-antibodies-ihc-testing-2.jpgAnti-TLS/FUS Antibody Picoband® IHC analysis of TLS/FUS using anti-TLS/FUS antibody (A00771-3). TLS/FUS was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TLS/FUS Antibody (A00771-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00771-3-fus-primary-antibodies-ihc-testing-3.jpgAnti-TLS/FUS Antibody Picoband® IHC analysis of TLS/FUS using anti-TLS/FUS antibody (A00771-3). TLS/FUS was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TLS/FUS Antibody (A00771-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00771-3-fus-primary-antibodies-ihc-testing-4.jpgAnti-TLS/FUS Antibody Picoband® IHC analysis of TLS/FUS using anti-TLS/FUS antibody (A00771-3). TLS/FUS was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TLS/FUS Antibody (A00771-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00771-3-fus-primary-antibodies-if-testing-5.jpgAnti-TLS/FUS Antibody Picoband® IF analysis of TLS/FUS using anti-TLS/FUS antibody (A00771-3) and anti-Tubulin alpha antibody (M03989-2). TLS/FUS was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TLS/FUS Antibody (A00771-3) and mouse anti-Tubulin alpha antibody (M03989-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00771-3-fus-primary-antibodies-fcm-testing-6.pngAnti-TLS/FUS Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-TLS/FUS antibody (A00771-3). Overlay histogram showing ANA-1 cells stained with A00771-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLS/FUS Antibody (A00771-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00771-3-fus-primary-antibodies-fcm-testing-7.pngAnti-TLS/FUS Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TLS/FUS antibody (A00771-3). Overlay histogram showing U87 cells stained with A00771-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLS/FUS Antibody (A00771-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-g3bp-g3bp1-picoband-trade-antibody-a02199-2-boster.html2026-03-17T05:12:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-wb-testing-1.jpgAnti-G3BP/G3BP1 Antibody Picoband® Western blot analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G3BP/G3BP1 antigen affinity purified polyclonal antibody (Catalog # A02199-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G3BP/G3BP1 at approximately 68 kDa. The expected band size for G3BP/G3BP1 is at 68 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-11.jpgAnti-G3BP/G3BP1 Antibody Picoband®IHC analysis of G3BP1 using anti-G3BP1 antibody (A02199-2). G3BP1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-2.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-3.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-4.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-5.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-6.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-7.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-8.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-ihc-testing-9.jpgAnti-G3BP/G3BP1 Antibody Picoband® IHC analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-if-testing-10.jpgAnti-G3BP/G3BP1 Antibody Picoband® IF analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). G3BP/G3BP1 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-G3BP/G3BP1 Antibody (A02199-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02199-2-g3bp1-primary-antibodies-fcm-testing-11.pngAnti-G3BP/G3BP1 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-G3BP/G3BP1 antibody (A02199-2). Overlay histogram showing Jurkat cells stained with A02199-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-G3BP/G3BP1 Antibody (A02199-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gadd45b-picoband-trade-antibody-a03508-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03508-1-gadd45b-primary-antibodies-wb-testing-1.jpgAnti-GADD45B Antibody Picoband® Western blot analysis of GADD45B using anti-GADD45B antibody (A03508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45B antigen affinity purified polyclonal antibody (Catalog # A03508-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45B at approximately 18 kDa. The expected band size for GADD45B is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03508-1-gadd45b-primary-antibodies-ihc-testing-2.jpgAnti-GADD45B Antibody Picoband® IHC analysis of GADD45B using anti-GADD45B antibody (A03508-1). GADD45B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GADD45B Antibody (A03508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03508-1-gadd45b-primary-antibodies-ihc-testing-3.jpgAnti-GADD45B Antibody Picoband® IHC analysis of GADD45B using anti-GADD45B antibody (A03508-1). GADD45B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GADD45B Antibody (A03508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03508-1-gadd45b-primary-antibodies-ihc-testing-4.jpgAnti-GADD45B Antibody Picoband® IHC analysis of GADD45B using anti-GADD45B antibody (A03508-1). GADD45B was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GADD45B Antibody (A03508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03508-1-gadd45b-primary-antibodies-ihc-testing-5.jpgAnti-GADD45B Antibody Picoband® IHC analysis of GADD45B using anti-GADD45B antibody (A03508-1). GADD45B was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GADD45B Antibody (A03508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-gas2-picoband-trade-antibody-a08589-1-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08589-1-gas2-primary-antibodies-wb-testing-1.jpgAnti-GAS2 Antibody Picoband® Western blot analysis of GAS2 using anti-GAS2 antibody (A08589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS2 antigen affinity purified polyclonal antibody (Catalog # A08589-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS2 at approximately 35 kDa. The expected band size for GAS2 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08589-1-gas2-primary-antibodies-fcm-testing-2_1.pngAnti-GAS2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-GAS2 antibody (A08589-1). Overlay histogram showing Jurkat cells stained with A08589-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAS2 Antibody (A08589-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gastrin-gast-picoband-trade-antibody-a03612-2-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03612-2-gast-primary-antibodies-wb-testing-1.jpgAnti-Gastrin/GAST Antibody Picoband® Western blot analysis of Gastrin/GAST using anti-Gastrin/GAST antibody (A03612-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastrin/GAST antigen affinity purified polyclonal antibody (Catalog # A03612-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gastrin/GAST at approximately 20 kDa. The expected band size for Gastrin/GAST is at 11 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03612-2-gast-primary-antibodies-fcm-testing-2.pngAnti-Gastrin/GAST Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-Gastrin/GAST antibody (A03612-2). Overlay histogram showing Caco-2 cells stained with A03612-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gastrin/GAST Antibody (A03612-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03612-2-gast-primary-antibodies-fcm-testing-3.pngAnti-Gastrin/GAST Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Gastrin/GAST antibody (A03612-2). Overlay histogram showing U87 cells stained with A03612-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gastrin/GAST Antibody (A03612-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gfpt1-picoband-trade-antibody-a04341-1-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-wb-testing-1.jpgAnti-GFPT1 Antibody Picoband® Western blot analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFPT1 antigen affinity purified polyclonal antibody (Catalog # A04341-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFPT1 at approximately 79 kDa. The expected band size for GFPT1 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-ihc-testing-2.jpgAnti-GFPT1 Antibody Picoband® IHC analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). GFPT1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFPT1 Antibody (A04341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-ihc-testing-3.jpgAnti-GFPT1 Antibody Picoband® IHC analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). GFPT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFPT1 Antibody (A04341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-ihc-testing-4.jpgAnti-GFPT1 Antibody Picoband® IHC analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). GFPT1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFPT1 Antibody (A04341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-ihc-testing-5.jpgAnti-GFPT1 Antibody Picoband® IHC analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). GFPT1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFPT1 Antibody (A04341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-ihc-testing-6.jpgAnti-GFPT1 Antibody Picoband® IHC analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). GFPT1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFPT1 Antibody (A04341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-ihc-testing-7.jpgAnti-GFPT1 Antibody Picoband® IHC analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). GFPT1 was detected in a paraffin-embedded section of human lienal rupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFPT1 Antibody (A04341-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-if-testing-8.jpgAnti-GFPT1 Antibody Picoband® IF analysis of GFPT1 using anti-GFPT1 antibody (A04341-1). GFPT1 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GFPT1 Antibody (A04341-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-fcm-testing-9.pngAnti-GFPT1 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-GFPT1 antibody (A04341-1). Overlay histogram showing Caco-2 cells stained with A04341-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GFPT1 Antibody (A04341-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04341-1-gfpt1-primary-antibodies-fcm-testing-10.pngAnti-GFPT1 Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-GFPT1 antibody (A04341-1). Overlay histogram showing NRK cells stained with A04341-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GFPT1 Antibody (A04341-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-growth-hormone-gh1-picoband-trade-antibody-a00851-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00851-gh1-primary-antibodies-wb-testing-1.jpgAnti-Growth Hormone/GH1 Antibody Picoband® Western blot analysis of Growth Hormone/GH1 using anti-Growth Hormone/GH1 antibody (A00851). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Growth Hormone/GH1 antigen affinity purified polyclonal antibody (Catalog # A00851) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Growth Hormone/GH1 at approximately 22 kDa. The expected band size for Growth Hormone/GH1 is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-gjb2-picoband-trade-antibody-a00512-2-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00512-2-gjb2-primary-antibodies-wb-testing-1.jpgAnti-GJB2 Antibody Picoband® Western blot analysis of GJB2 using anti-GJB2 antibody (A00512-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJB2 antigen affinity purified polyclonal antibody (Catalog # A00512-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJB2 at approximately 26 kDa. The expected band size for GJB2 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00512-2-13046_2019_1142_fig1_html.pngAnti-GJB2 Antibody Picoband®Expression and distribution of Cx32, Cx26 and Cx43 in patients with HCC. a. The protein expression levels of Cx32, Cx26 and Cx43 were determined by western blot analysis. β-actin was used as the loading control. b. The expression of Cx32 was correlated with increased TNM stages, as revealed by western blot analysis. β-actin was used as the loading control. c . Statistical analysis of the relative expression levels of Cxs in HCC tissues, peritumoral tissues, and normal liver tissues. ** , P < 0.01; ## , P < 0.01. d. Statistical analysis of the relative expression levels of Cx32 in peritumoral tissues and HCC tissues with different TNM stages. * , P < 0.05. e. Representative IHC staining of Cx32, Cx26 and Cx43 protein in normal liver tissues (left panels), peritumoral tissues (middle panels) and HCC tissues (right panels) (400×). Scale bars: 50 μm. f. Representative IHC staining of Cx32 in normal liver tissues, cirrhotic tissues and early and advanced HCC tissues (400×). Scale bars: 50 μm Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00512-2-gjb2-primary-antibodies-fcm-testing-2.pngAnti-GJB2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-GJB2 antibody (A00512-2). Overlay histogram showing PC-3 cells stained with A00512-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GJB2 Antibody (A00512-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00512-2-gjb2-primary-antibodies-fcm-testing-3.pngAnti-GJB2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-GJB2 antibody (A00512-2). Overlay histogram showing U251 cells stained with A00512-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GJB2 Antibody (A00512-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gls2-picoband-trade-antibody-a05334-1-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05334-1-gls2-primary-antibodies-wb-testing-1.jpgAnti-GLS2 Antibody Picoband® Western blot analysis of GLS2 using anti-GLS2 antibody (A05334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLS2 antigen affinity purified polyclonal antibody (Catalog # A05334-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLS2 at approximately 66 kDa. The expected band size for GLS2 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05334-1-gls2-primary-antibodies-fcm-testing-2.pngAnti-GLS2 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-GLS2 antibody (A05334-1). Overlay histogram showing Caco-2 cells stained with A05334-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLS2 Antibody (A05334-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-grhl2-picoband-trade-antibody-a04120-2-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-wb-testing-1.jpgAnti-GRHL2 Antibody Picoband® Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-ihc-testing-2.jpgAnti-GRHL2 Antibody Picoband® IHC analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). GRHL2 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRHL2 Antibody (A04120-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-ihc-testing-3.jpgAnti-GRHL2 Antibody Picoband® IHC analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). GRHL2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRHL2 Antibody (A04120-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-ihc-testing-4.jpgAnti-GRHL2 Antibody Picoband® IHC analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). GRHL2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRHL2 Antibody (A04120-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-ihc-testing-5.jpgAnti-GRHL2 Antibody Picoband® IHC analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). GRHL2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRHL2 Antibody (A04120-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-if-testing-6.jpgAnti-GRHL2 Antibody Picoband® IF analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). GRHL2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GRHL2 Antibody (A04120-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-fcm-testing-7.pngAnti-GRHL2 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-GRHL2 antibody (A04120-2). Overlay histogram showing Caco-2 cells stained with A04120-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRHL2 Antibody (A04120-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04120-2-grhl2-primary-antibodies-fcm-testing-8.pngAnti-GRHL2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-GRHL2 antibody (A04120-2). Overlay histogram showing MCF-7 cells stained with A04120-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRHL2 Antibody (A04120-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-histidine-decarboxylase-hdc-picoband-trade-antibody-a01876-1-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01876-1-hdc-primary-antibodies-wb-testing-1.jpgAnti-Histidine decarboxylase/HDC Antibody Picoband® Western blot analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (A01876-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histidine Decarboxylase/HDC antigen affinity purified polyclonal antibody (Catalog # A01876-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Histidine Decarboxylase/HDC at approximately 74 kDa. The expected band size for Histidine Decarboxylase/HDC is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01876-1-hdc-primary-antibodies-ihc-testing-2.jpgAnti-Histidine decarboxylase/HDC Antibody Picoband® IHC analysis of Histidine Decarboxylase/HDC using anti-Histidine Decarboxylase/HDC antibody (A01876-1). Histidine Decarboxylase/HDC was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histidine Decarboxylase/HDC Antibody (A01876-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-impdh1-picoband-trade-antibody-a03791-1-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-wb-testing-1.jpgAnti-IMPDH1 Antibody Picoband® Western blot analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMPDH1 antigen affinity purified polyclonal antibody (Catalog # A03791-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMPDH1 at approximately 55 kDa. The expected band size for IMPDH1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-ihc-testing-2.jpgAnti-IMPDH1 Antibody Picoband® IHC analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). IMPDH1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH1 Antibody (A03791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-ihc-testing-3.jpgAnti-IMPDH1 Antibody Picoband® IHC analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). IMPDH1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH1 Antibody (A03791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-ihc-testing-4.jpgAnti-IMPDH1 Antibody Picoband® IHC analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). IMPDH1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH1 Antibody (A03791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-ihc-testing-5.jpgAnti-IMPDH1 Antibody Picoband® IHC analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). IMPDH1 was detected in a paraffin-embedded section of human rupture of spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH1 Antibody (A03791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-ihc-testing-6.jpgAnti-IMPDH1 Antibody Picoband® IHC analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). IMPDH1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH1 Antibody (A03791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-if-testing-7.jpgAnti-IMPDH1 Antibody Picoband® IF analysis of IMPDH1 using anti-IMPDH1 antibody (A03791-1). IMPDH1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IMPDH1 Antibody (A03791-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03791-1-impdh1-primary-antibodies-fcm-testing-8.pngAnti-IMPDH1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-IMPDH1 antibody (A03791-1). Overlay histogram showing THP-1 cells stained with A03791-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IMPDH1 Antibody (A03791-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-kir3dl1-picoband-trade-antibody-a02187-2-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02187-2-kir3dl1-primary-antibodies-wb-testing-1.jpgAnti-KIR3DL1 Antibody Picoband® Western blot analysis of KIR3DL1 using anti-KIR3DL1 antibody (A02187-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIR3DL1 antigen affinity purified polyclonal antibody (Catalog # A02187-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIR3DL1 at approximately 49 kDa. The expected band size for KIR3DL1 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-klf4-picoband-trade-antibody-a00120-3-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00120-3-klf4-primary-antibodies-wb-testing-1.jpgAnti-KLF4 Antibody Picoband® Western blot analysis of KLF4 using anti-KLF4 antibody (A00120-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF4 antigen affinity purified polyclonal antibody (Catalog # A00120-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF4 at approximately 60 kDa. The expected band size for KLF4 is at 60 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00120-3-klf4-primary-antibodies-ihc-testing-2..jpgAnti-KLF4 Antibody Picoband® IHC analysis of KLF4 using anti-KLF4 antibody (A00120-3). KLF4 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLF4 Antibody (A00120-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00120-3-klf4-primary-antibodies-ihc-testing-3.jpgAnti-KLF4 Antibody Picoband® IHC analysis of KLF4 using anti-KLF4 antibody (A00120-3). KLF4 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLF4 Antibody (A00120-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00120-3-klf4-primary-antibodies-ihc-testing-4.jpgAnti-KLF4 Antibody Picoband®IHC analysis of KLF4 using anti-KLF4 antibody (A00120-3). KLF4 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLF4 Antibody (A00120-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00120-3-klf4-primary-antibodies-fcm-testing-4.pngAnti-KLF4 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-KLF4 antibody (A00120-3). Overlay histogram showing Hela cells stained with A00120-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KLF4 Antibody (A00120-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-kpna2-picoband-trade-antibody-a01776-3-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01776-3-kpna2-primary-antibodies-wb-testing-1.jpgAnti-KPNA2 Antibody Picoband® Western blot analysis of KPNA2 using anti-KPNA2 antibody (A01776-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KPNA2 antigen affinity purified polyclonal antibody (Catalog # A01776-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KPNA2 at approximately 58 kDa. The expected band size for KPNA2 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01776-3-kpna2-primary-antibodies-ihc-testing-2.jpgAnti-KPNA2 Antibody Picoband® IHC analysis of KPNA2 using anti-KPNA2 antibody (A01776-3). KPNA2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KPNA2 Antibody (A01776-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01776-3-kpna2-primary-antibodies-ihc-testing-3.jpgAnti-KPNA2 Antibody Picoband® IHC analysis of KPNA2 using anti-KPNA2 antibody (A01776-3). KPNA2 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KPNA2 Antibody (A01776-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01776-3-kpna2-primary-antibodies-ihc-testing-4.jpgAnti-KPNA2 Antibody Picoband® IHC analysis of KPNA2 using anti-KPNA2 antibody (A01776-3). KPNA2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KPNA2 Antibody (A01776-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01776-3-kpna2-primary-antibodies-ihc-testing-5.jpgAnti-KPNA2 Antibody Picoband® IHC analysis of KPNA2 using anti-KPNA2 antibody (A01776-3). KPNA2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KPNA2 Antibody (A01776-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01776-3-kpna2-primary-antibodies-ihc-testing-6.jpgAnti-KPNA2 Antibody Picoband® IHC analysis of KPNA2 using anti-KPNA2 antibody (A01776-3). KPNA2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KPNA2 Antibody (A01776-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01776-3-kpna2-primary-antibodies-fcm-testing-7.pngAnti-KPNA2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-KPNA2 antibody (A01776-3). Overlay histogram showing U87 cells stained with A01776-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KPNA2 Antibody (A01776-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-krt7-picoband-trade-antibody-a02416-2-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-wb-testing-1.jpgAnti-KRT7 Antibody Picoband® Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-ihc-testing-2.jpgAnti-KRT7 Antibody Picoband® IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-ihc-testing-3.jpgAnti-KRT7 Antibody Picoband® IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-ihc-testing-4.jpgAnti-KRT7 Antibody Picoband® IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-ihc-testing-5.pngAnti-KRT7 Antibody Picoband® IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-ihc-testing-6.pngAnti-KRT7 Antibody Picoband® IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-ihc-testing-7.pngAnti-KRT7 Antibody Picoband® IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-if-testing-8.pngAnti-KRT7 Antibody Picoband® IF analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human throid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-if-testing-9.pngAnti-KRT7 Antibody Picoband® IF analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-if-testing-10.pngAnti-KRT7 Antibody Picoband® IF analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-if-testing-11.pngAnti-KRT7 Antibody Picoband® IF analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02416-2-krt7-primary-antibodies-if-testing-12.pngAnti-KRT7 Antibody Picoband® IF analysis of KRT7 using anti-KRT7 antibody (A02416-2). KRT7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-l1re1-picoband-trade-antibody-a13161-boster.html2026-03-17T05:12:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13161-l1re1-primary-antibodies-wb-testing-1.jpgAnti-L1RE1 Antibody Picoband® Western blot analysis of L1RE1 using anti-L1RE1 antibody (A13161). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L1RE1 antigen affinity purified polyclonal antibody (Catalog # A13161) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L1RE1 at approximately 40 kDa. The expected band size for L1RE1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13161-l1re1-primary-antibodies-fcm-testing-2.pngAnti-L1RE1 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-L1RE1 antibody (A13161). Overlay histogram showing Daudi cells stained with A13161 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-L1RE1 Antibody (A13161, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-lnpep-picoband-trade-antibody-a05092-1-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05092-1-lnpep-primary-antibodies-wb-testing-1.jpgAnti-LNPEP Antibody Picoband® Western blot analysis of LNPEP using anti-LNPEP antibody (A05092-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LNPEP antigen affinity purified polyclonal antibody (Catalog # A05092-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LNPEP at approximately 117 kDa. The expected band size for LNPEP is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05092-1-lnpep-primary-antibodies-ihc-testing-2.jpgAnti-LNPEP Antibody Picoband® IHC analysis of LNPEP using anti-LNPEP antibody (A05092-1). LNPEP was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LNPEP Antibody (A05092-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05092-1-lnpep-primary-antibodies-ihc-testing-3.jpgAnti-LNPEP Antibody Picoband® IHC analysis of LNPEP using anti-LNPEP antibody (A05092-1). LNPEP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LNPEP Antibody (A05092-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05092-1-lnpep-primary-antibodies-ihc-testing-4.jpgAnti-LNPEP Antibody Picoband® IHC analysis of LNPEP using anti-LNPEP antibody (A05092-1). LNPEP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LNPEP Antibody (A05092-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-mavs-picoband-trade-antibody-a00169-4-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00169-4-mavs-primary-antibodies-wb-testing-1.jpgAnti-Mavs Antibody Picoband® Western blot analysis of Mavs using anti-Mavs antibody (A00169-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mavs antigen affinity purified polyclonal antibody (Catalog # A00169-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mavs at approximately 57 kDa. The expected band size for Mavs is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00169-4-mavs-primary-antibodies-fcm-testing-2.pngAnti-Mavs Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Mavs antibody (A00169-4). Overlay histogram showing ANA-1 cells stained with A00169-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mavs Antibody (A00169-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mlx-picoband-trade-antibody-a03087-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03087-2-mlx-primary-antibodies-wb-testing-1.jpgAnti-MLX Antibody Picoband® Western blot analysis of MLX using anti-MLX antibody (A03087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX antigen affinity purified polyclonal antibody (Catalog # A03087-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLX at approximately 50 kDa. The expected band size for MLX is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03087-2-mlx-primary-antibodies-fcm-testing-2.pngAnti-MLX Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-MLX antibody (A03087-2). Overlay histogram showing THP-1 cells stained with A03087-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MLX Antibody (A03087-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-muc1-picoband-trade-antibody-a00187-3-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00187-3-muc1-primary-antibodies-ihc-testing-1.jpgAnti-MUC1 Antibody IHC analysis of MUC1 using anti-MUC1 antibody (A00187-3). MUC1 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC1 Antibody (A00187-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00187-3-muc1-primary-antibodies-fcm-testing-2.pngAnti-MUC1 Antibody Flow Cytometry analysis of A431 cells using anti-MUC1 antibody (A00187-3). Overlay histogram showing A431 cells stained with A00187-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MUC1 Antibody (A00187-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nedd4-picoband-trade-antibody-a00984-3-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-wb-testing-1.jpgAnti-NEDD4 Antibody Picoband® Western blot analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4 antigen affinity purified polyclonal antibody (Catalog # A00984-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4 at approximately 115 kDa. The expected band size for NEDD4 is at 115 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-ihc-testing-7.jpgAnti-NEDD4 Antibody Picoband®IHC analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). NEDD4 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD4 Antibody (A00984-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-ihc-testing-2.jpgAnti-NEDD4 Antibody Picoband® IHC analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). NEDD4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD4 Antibody (A00984-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-ihc-testing-3.jpgAnti-NEDD4 Antibody Picoband® IHC analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). NEDD4 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD4 Antibody (A00984-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-ihc-testing-4.jpgAnti-NEDD4 Antibody Picoband® IHC analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). NEDD4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD4 Antibody (A00984-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-ihc-testing-5.jpgAnti-NEDD4 Antibody Picoband® IHC analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). NEDD4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD4 Antibody (A00984-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-ihc-testing-6.jpgAnti-NEDD4 Antibody Picoband® IHC analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). NEDD4 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD4 Antibody (A00984-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-if-testing-7.jpgAnti-NEDD4 Antibody Picoband® IF analysis of NEDD4 using anti-NEDD4 antibody (A00984-3). NEDD4 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NEDD4 Antibody (A00984-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-3-nedd4-primary-antibodies-fcm-testing-8.pngAnti-NEDD4 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-NEDD4 antibody (A00984-3). Overlay histogram showing U87 cells stained with A00984-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD4 Antibody (A00984-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nlrp7-picoband-trade-antibody-a07838-1-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07838-1-nlrp7-primary-antibodies-wb-testing-1.jpgAnti-NLRP7 Antibody Picoband® Western blot analysis of NLRP7 using anti-NLRP7 antibody (A07838-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP7 antigen affinity purified polyclonal antibody (Catalog # A07838-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP7 at approximately 112 kDa. The expected band size for NLRP7 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07838-1-nlrp7-primary-antibodies-ihc-testing-2.jpgAnti-NLRP7 Antibody Picoband® IHC analysis of NLRP7 using anti-NLRP7 antibody (A07838-1). NLRP7 was detected in a paraffin-embedded section of human lienal rupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRP7 Antibody (A07838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07838-1-nlrp7-primary-antibodies-ihc-testing-3.jpgAnti-NLRP7 Antibody Picoband® IHC analysis of NLRP7 using anti-NLRP7 antibody (A07838-1). NLRP7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRP7 Antibody (A07838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07838-1-nlrp7-primary-antibodies-ihc-testing-4.jpgAnti-NLRP7 Antibody Picoband® IHC analysis of NLRP7 using anti-NLRP7 antibody (A07838-1). NLRP7 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRP7 Antibody (A07838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07838-1-nlrp7-primary-antibodies-ihc-testing-5.jpgAnti-NLRP7 Antibody Picoband® IHC analysis of NLRP7 using anti-NLRP7 antibody (A07838-1). NLRP7 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRP7 Antibody (A07838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07838-1-nlrp7-primary-antibodies-fcm-testing-6_1.pngAnti-NLRP7 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-NLRP7 antibody (A07838-1). Overlay histogram showing Daudi cells stained with A07838-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP7 Antibody (A07838-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-p2y6-p2ry6-picoband-trade-antibody-a10087-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10087-2-p2ry6-primary-antibodies-wb-testing-1.jpgAnti-P2Y6/P2RY6 Antibody Picoband® Western blot analysis of P2Y6/P2RY6 using anti-P2Y6/P2RY6 antibody (A10087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2Y6/P2RY6 antigen affinity purified polyclonal antibody (Catalog # A10087-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2Y6/P2RY6 at approximately 45 kDa. The expected band size for P2Y6/P2RY6 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rab13-picoband-trade-antibody-a04339-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-wb-testing-1_1.jpgAnti-RAB13 Antibody Picoband® Western blot analysis of RAB13 using anti-RAB13 antibody (A04339). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB13 antigen affinity purified polyclonal antibody (Catalog # A04339) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB13 at approximately 23 kDa. The expected band size for RAB13 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-ihc-testing-2_1.jpgAnti-RAB13 Antibody Picoband® IHC analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-fcm-testing-10.pngAnti-RAB13 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-RAB13 antibody (A04339). Overlay histogram showing U87 cells stained with A04339 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB13 Antibody (A04339, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-ihc-testing-3_1.jpgAnti-RAB13 Antibody Picoband® IHC analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-ihc-testing-4_1.jpgAnti-RAB13 Antibody Picoband® IHC analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-ihc-testing-5_1.jpgAnti-RAB13 Antibody Picoband® IHC analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-ihc-testing-6_1.jpgAnti-RAB13 Antibody Picoband® IHC analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-ihc-testing-7.jpgAnti-RAB13 Antibody Picoband® IHC analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-ihc-testing-8.jpgAnti-RAB13 Antibody Picoband® IHC analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04339-rab13-primary-antibodies-if-testing-9.jpgAnti-RAB13 Antibody Picoband® IF analysis of RAB13 using anti-RAB13 antibody (A04339). RAB13 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB13 Antibody (A04339) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-hhr23a-rad23a-picoband-trade-antibody-a03243-3-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03243-3-rad23a-primary-antibodies-wb-testing-1.jpgAnti-hHR23A/RAD23A Antibody Picoband® Western blot analysis of HHR23A/RAD23A using anti-HHR23A/RAD23A antibody (A03243-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HHR23A/RAD23A antigen affinity purified polyclonal antibody (Catalog # A03243-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HHR23A/RAD23A at approximately 60 kDa. The expected band size for HHR23A/RAD23A is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03243-3-rad23a-primary-antibodies-fcm-testing-2.pngAnti-hHR23A/RAD23A Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-HHR23A/RAD23A antibody (A03243-3). Overlay histogram showing ANA-1 cells stained with A03243-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HHR23A/RAD23A Antibody (A03243-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03243-3-rad23a-primary-antibodies-fcm-testing-3.pngAnti-hHR23A/RAD23A Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-HHR23A/RAD23A antibody (A03243-3). Overlay histogram showing C6 cells stained with A03243-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HHR23A/RAD23A Antibody (A03243-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03243-3-rad23a-primary-antibodies-fcm-testing-4.pngAnti-hHR23A/RAD23A Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-HHR23A/RAD23A antibody (A03243-3). Overlay histogram showing Hela cells stained with A03243-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HHR23A/RAD23A Antibody (A03243-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-neun-rbfox3-picoband-trade-antibody-a11954-1-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11954-1-rbfox3-primary-antibodies-ihc-testing-4.jpgAnti-NeuN/Rbfox3 Antibody Picoband®IHC analysis of NeuN/RBFOX3 using anti-NeuN/RBFOX3 antibody (A11954-1). NeuN/RBFOX3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NeuN/RBFOX3 Antibody (A11954-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11954-1-rbfox3-primary-antibodies-wb-testing-1_1.jpgAnti-NeuN/Rbfox3 Antibody Picoband® Western blot analysis of NeuN/Rbfox3 using anti-NeuN/Rbfox3 antibody (A11954-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NeuN/Rbfox3 antigen affinity purified polyclonal antibody (Catalog # A11954-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NeuN/Rbfox3 at approximately 46-55 kDa. The expected band size for NeuN/Rbfox3 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11954-1-rbfox3-primary-antibodies-ihc-testing-2.jpgAnti-NeuN/Rbfox3 Antibody Picoband® IHC analysis of NeuN/Rbfox3 using anti-NeuN/Rbfox3 antibody (A11954-1). NeuN/Rbfox3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NeuN/Rbfox3 Antibody (A11954-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11954-1-rbfox3-primary-antibodies-ihc-testing-3.jpgAnti-NeuN/Rbfox3 Antibody Picoband® IHC analysis of NeuN/Rbfox3 using anti-NeuN/Rbfox3 antibody (A11954-1). NeuN/Rbfox3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NeuN/Rbfox3 Antibody (A11954-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11954-1-rbfox3-primary-antibodies-if-testing-4.jpgAnti-NeuN/Rbfox3 Antibody Picoband® IF analysis of NeuN/Rbfox3 using anti-NeuN/Rbfox3 antibody (A11954-1). NeuN/Rbfox3 was detected in a paraffin-embedded section of rat brain cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NeuN/Rbfox3 Antibody (A11954-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rip2-ripk2-picoband-trade-antibody-a00818-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-2-ripk2-primary-antibodies-fcm-testing-2.pngAnti-RIP2/RIPK2 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-RIP2/RIPK2 antibody (A00818-2). Overlay histogram showing Caco-2 cells stained with A00818-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIP2/RIPK2 Antibody (A00818-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-2-ripk2-primary-antibodies-wb-testing-1.jpgAnti-RIP2/RIPK2 Antibody Picoband® Western blot analysis of RIP2/RIPK2 using anti-RIP2/RIPK2 antibody (A00818-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP2/RIPK2 antigen affinity purified polyclonal antibody (Catalog # A00818-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP2/RIPK2 at approximately 61 kDa. The expected band size for RIP2/RIPK2 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-2-ripk2-primary-antibodies-ihc-testing-1.jpgAnti-RIP2/RIPK2 Antibody Picoband®IHC analysis of RIPK2 using anti- RIPK2 antibody (A00818-2). RIPK2 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti- RIPK2 Antibody (A00818-2) at 5 μg/ml overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00818-2-ripk2-primary-antibodies-ihc-testing-2.jpgAnti-RIP2/RIPK2 Antibody Picoband®IHC analysis of RIPK2 using anti- RIPK2 antibody (A00818-2). RIPK2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with rabbit anti- RIPK2 Antibody (A00818-2) at 5 μg/ml overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-s6k1-rps6kb1-picoband-trade-antibody-a01475-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01475-2-rps6kb1-primary-antibodies-wb-testing-1.jpgAnti-S6K1/RPS6KB1 Antibody Picoband® Western blot analysis of S6K1/RPS6KB1 using anti-S6K1/RPS6KB1 antibody (A01475-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S6K1/RPS6KB1 antigen affinity purified polyclonal antibody (Catalog # A01475-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S6K1/RPS6KB1 at approximately 59 kDa. The expected band size for S6K1/RPS6KB1 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01475-2-rps6kb1-primary-antibodies-fcm-testing-2.pngAnti-S6K1/RPS6KB1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-S6K1/RPS6KB1 antibody (A01475-2). Overlay histogram showing MCF-7 cells stained with A01475-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S6K1/RPS6KB1 Antibody (A01475-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rrm1-picoband-trade-antibody-a01764-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01764-2-rrm1-primary-antibodies-wb-testing-1.jpgAnti-RRM1 Antibody Picoband® Western blot analysis of RRM1 using anti-RRM1 antibody (A01764-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM1 antigen affinity purified polyclonal antibody (Catalog # A01764-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRM1 at approximately 90 kDa. The expected band size for RRM1 is at 90 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01764-2-rrm1-primary-antibodies-fcm-testing-2.pngAnti-RRM1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-RRM1 antibody (A01764-2). Overlay histogram showing U87 cells stained with A01764-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRM1 Antibody (A01764-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rrs1-picoband-trade-antibody-a04418-3-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-wb-testing-1.jpgAnti-RRS1 Antibody Picoband® Western blot analysis of RRS1 using anti-RRS1 antibody (A04418-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human MDA-MB-453 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: rat RH35 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates, Lane 10: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRS1 antigen affinity purified polyclonal antibody (Catalog # A04418-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRS1 at approximately 39 kDa. The expected band size for RRS1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-ihc-testing-2.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-3). RRS1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-ihc-testing-3.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-3). RRS1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-ihc-testing-4.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-3). RRS1 was detected in a paraffin-embedded section of human lienal rupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-ihc-testing-5.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-3). RRS1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-ihc-testing-6.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-3). RRS1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-if-testing-7.jpgAnti-RRS1 Antibody Picoband® IF analysis of RRS1 using anti-RRS1 antibody (A04418-3). RRS1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RRS1 Antibody (A04418-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-3-rrs1-primary-antibodies-fcm-testing-8.pngAnti-RRS1 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-RRS1 antibody (A04418-3). Overlay histogram showing Caco-2 cells stained with A04418-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRS1 Antibody (A04418-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-alpha-2-antiplasmin-serpinf2-picoband-trade-antibody-a04248-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04248-2-serpinf2-primary-antibodies-wb-testing-1.jpgAnti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® Western blot analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha 2 Antiplasmin/SERPINF2 antigen affinity purified polyclonal antibody (Catalog # A04248-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha 2 Antiplasmin/SERPINF2 at approximately 65 kDa. The expected band size for Alpha 2 Antiplasmin/SERPINF2 is at 65 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04248-2-serpinf2-primary-antibodies-ihc-testing-5.jpgAnti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband®IHC analysis of Alpha 2-Antiplasmin/SERPINF2 using anti-Alpha 2-Antiplasmin/SERPINF2 antibody (A04248-2). Alpha 2-Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2-Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04248-2-serpinf2-primary-antibodies-ihc-testing-2.jpgAnti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2). Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04248-2-serpinf2-primary-antibodies-ihc-testing-3.jpgAnti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2). Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04248-2-serpinf2-primary-antibodies-ihc-testing-4.jpgAnti-Alpha 2 Antiplasmin/SERPINF2 Antibody Picoband® IHC analysis of Alpha 2 Antiplasmin/SERPINF2 using anti-Alpha 2 Antiplasmin/SERPINF2 antibody (A04248-2). Alpha 2 Antiplasmin/SERPINF2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha 2 Antiplasmin/SERPINF2 Antibody (A04248-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-sigma1-receptor-sigmar1-picoband-trade-antibody-a02493-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02493-2-sigmar1-primary-antibodies-wb-testing-1.jpgAnti-Sigma1-receptor/SIGMAR1 Antibody Picoband® Western blot analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (A02493-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human COLO-320 whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: monkey COS-7 whole cell lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sigma1-Receptor/SIGMAR1 antigen affinity purified polyclonal antibody (Catalog # A02493-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sigma1-Receptor/SIGMAR1 at approximately 25 kDa. The expected band size for Sigma1-Receptor/SIGMAR1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02493-2-sigmar1-primary-antibodies-ihc-testing-7.jpgAnti-Sigma1-receptor/SIGMAR1 Antibody Picoband®IHC analysis of SIGMAR1 using anti-SIGMAR1 antibody (A02493-2). SIGMAR1 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIGMAR1 Antibody (A02493-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02493-2-sigmar1-primary-antibodies-ihc-testing-2.jpgAnti-Sigma1-receptor/SIGMAR1 Antibody Picoband® IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (A02493-2). Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (A02493-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02493-2-sigmar1-primary-antibodies-ihc-testing-3.jpgAnti-Sigma1-receptor/SIGMAR1 Antibody Picoband® IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (A02493-2). Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (A02493-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02493-2-sigmar1-primary-antibodies-ihc-testing-4.jpgAnti-Sigma1-receptor/SIGMAR1 Antibody Picoband® IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (A02493-2). Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (A02493-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02493-2-sigmar1-primary-antibodies-ihc-testing-5.jpgAnti-Sigma1-receptor/SIGMAR1 Antibody Picoband® IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (A02493-2). Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of human stomach cancer tissue was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (A02493-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02493-2-sigmar1-primary-antibodies-ihc-testing-6.jpgAnti-Sigma1-receptor/SIGMAR1 Antibody Picoband® IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (A02493-2). Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of human liver cancer tissue was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (A02493-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-slam-cd150-slamf1-picoband-trade-antibody-a03988-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03988-2-slamf1-primary-antibodies-ihc-testing-1.jpgAnti-SLAM/CD150/SLAMF1 Antibody IHC analysis of SLAM/CD150/SLAMF1 using anti-SLAM/CD150/SLAMF1 antibody (A03988-2). SLAM/CD150/SLAMF1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLAM/CD150/SLAMF1 Antibody (A03988-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03988-2-slamf1-primary-antibodies-fcm-testing-2.pngAnti-SLAM/CD150/SLAMF1 Antibody Flow Cytometry analysis of U937 cells using anti-SLAM/CD150/SLAMF1 antibody (A03988-2). Overlay histogram showing U937 cells stained with A03988-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLAM/CD150/SLAMF1 Antibody (A03988-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dopamine-transporter-slc6a3-picoband-trade-antibody-a00369-3-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00369-3-slc6a3-primary-antibodies-wb-testing-1.jpgAnti-Dopamine Transporter/SLC6A3 Antibody Picoband® Western blot analysis of Dopamine Transporter/SLC6A3 using anti-Dopamine Transporter/SLC6A3 antibody (A00369-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dopamine Transporter/SLC6A3 antigen affinity purified polyclonal antibody (Catalog # A00369-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dopamine Transporter/SLC6A3 at approximately 68 kDa. The expected band size for Dopamine Transporter/SLC6A3 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00369-3-slc6a3-primary-antibodies-fcm-testing-2.pngAnti-Dopamine Transporter/SLC6A3 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Dopamine Transporter/SLC6A3 antibody (A00369-3). Overlay histogram showing THP-1 cells stained with A00369-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dopamine Transporter/SLC6A3 Antibody (A00369-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc10a1-ntcp1-picoband-trade-antibody-a06872-2-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06872-2-slc10a1-primary-antibodies-wb-testing-1.jpgAnti-SLC10A1/NTCP1 Antibody Picoband® Western blot analysis of SLC10A1/NTCP1 using anti-SLC10A1/NTCP1 antibody (A06872-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey liver tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1/NTCP1 antigen affinity purified polyclonal antibody (Catalog # A06872-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1/NTCP1 at approximately 38 kDa. The expected band size for SLC10A1/NTCP1 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc25a51-52-picoband-trade-antibody-a17353-boster.html2026-03-17T05:12:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17353-slc25a51-52-primary-antibodies-wb-testing-1.jpgAnti-SLC25A51/52 Antibody Picoband® Western blot analysis of SLC25A51/52 using anti-SLC25A51/52 antibody (A17353). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A51/52 antigen affinity purified polyclonal antibody (Catalog # A17353) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A51/52 at approximately 45 kDa. The expected band size for SLC25A51/52 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17353-slc25a51-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A51/52 Antibody Picoband® IHC analysis of SLC25A51/52 using anti-SLC25A51/52 antibody (A17353). SLC25A51/52 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A51/52 Antibody (A17353) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17353-slc25a51-primary-antibodies-ihc-testing-3.jpgAnti-SLC25A51/52 Antibody Picoband® IHC analysis of SLC25A51/52 using anti-SLC25A51/52 antibody (A17353). SLC25A51/52 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A51/52 Antibody (A17353) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17353-slc25a51-primary-antibodies-fcm-testing-4.pngAnti-SLC25A51/52 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-SLC25A51/52 antibody (A17353). Overlay histogram showing U251 cells stained with A17353 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC25A51/52 Antibody (A17353, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17353-slc25a51-primary-antibodies-if-testing-5.jpgAnti-SLC25A51/52 Antibody Picoband® IF analysis of SLC25A51/52 using anti-SLC25A51/52 antibody (A17353). SLC25A51/52 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC25A51/52 Antibody (A17353) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-sp6-picoband-trade-antibody-a08730-2-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08730-2-sp6-primary-antibodies-wb-testing-1.jpgAnti-SP6 Antibody Picoband® Western blot analysis of SP6 using anti-SP6 antibody (A08730-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP6 antigen affinity purified polyclonal antibody (Catalog # A08730-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP6 at approximately 40 kDa. The expected band size for SP6 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08730-2-sp6-primary-antibodies-if-testing-2.jpgAnti-SP6 Antibody Picoband® IF analysis of SP6 using anti-SP6 antibody (A08730-2). SP6 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SP6 Antibody (A08730-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08730-2-sp6-primary-antibodies-fcm-testing-3_1.pngAnti-SP6 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-SP6 antibody (A08730-2). Overlay histogram showing Caco-2 cells stained with A08730-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SP6 Antibody (A08730-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pu-1-spi1-picoband-trade-antibody-a01116-1-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01116-1-spi1-primary-antibodies-wb-testing-1.jpgAnti-PU.1/SPI1 Antibody Picoband® Western blot analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PU.1/SPI1 antigen affinity purified polyclonal antibody (Catalog # A01116-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PU.1/SPI1 at approximately 40 kDa. The expected band size for PU.1/SPI1 is at 40 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01116-1-spi1-primary-antibodies-ihc-testing-2.jpgAnti-PU.1/SPI1 Antibody Picoband® IHC analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). PU.1/SPI1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PU.1/SPI1 Antibody (A01116-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01116-1-spi1-primary-antibodies-ihc-testing-3.jpgAnti-PU.1/SPI1 Antibody Picoband® IHC analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). PU.1/SPI1 was detected in a paraffin-embedded section of mouse lymph gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PU.1/SPI1 Antibody (A01116-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01116-1-spi1-primary-antibodies-ihc-testing-4.jpgAnti-PU.1/SPI1 Antibody Picoband® IHC analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). PU.1/SPI1 was detected in a paraffin-embedded section of rat lymph gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PU.1/SPI1 Antibody (A01116-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01116-1-spi1-primary-antibodies-fcm-testing-5.pngAnti-PU.1/SPI1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PU.1/SPI1 antibody (A01116-1). Overlay histogram showing THP-1 cells stained with A01116-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PU.1/SPI1 Antibody (A01116-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tff1-picoband-trade-antibody-a01391-4-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01391-4-tff1-primary-antibodies-wb-testing-1.jpgAnti-TFF1 Antibody Picoband® Western blot analysis of TFF1 using anti-TFF1 antibody (A01391-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFF1 antigen affinity purified polyclonal antibody (Catalog # A01391-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFF1 at approximately 12 kDa. The expected band size for TFF1 is at 9 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01391-4-tff1-primary-antibodies-if-testing-2.jpgAnti-TFF1 Antibody Picoband® IF analysis of TFF1 using anti-TFF1 antibody (A01391-4). TFF1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TFF1 Antibody (A01391-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tsta3-gfus-picoband-trade-antibody-a09921-3-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09921-3-tsta3-primary-antibodies-wb-testing-1.jpgAnti-TSTA3/GFUS Antibody Picoband® Western blot analysis of TSTA3/GFUS using anti-TSTA3/GFUS antibody (A09921-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: mouse testis tissue lysates, Lane 10: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSTA3/GFUS antigen affinity purified polyclonal antibody (Catalog # A09921-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSTA3/GFUS at approximately 39 kDa. The expected band size for TSTA3/GFUS is at 39 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09921-3-gfus-primary-antibodies-ihc-testing-1.jpgAnti-TSTA3/GFUS Antibody Picoband®IHC analysis of GFUS using anti-GFUS antibody (A09921-3). GFUS was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFUS Antibody (A09921-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09921-3-tsta3-primary-antibodies-if-testing-2.jpgAnti-TSTA3/GFUS Antibody Picoband® IF analysis of TSTA3/GFUS using anti-TSTA3/GFUS antibody (A09921-3). TSTA3/GFUS was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSTA3/GFUS Antibody (A09921-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09921-3-tsta3-primary-antibodies-fcm-testing-3.pngAnti-TSTA3/GFUS Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TSTA3/GFUS antibody (A09921-3). Overlay histogram showing U87 cells stained with A09921-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSTA3/GFUS Antibody (A09921-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wapl-foe-picoband-trade-antibody-a03684-2-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03684-2-wapl-primary-antibodies-wb-testing-1.jpgAnti-WAPL/FOE Antibody Picoband® Western blot analysis of WAPL/FOE using anti-WAPL/FOE antibody (A03684-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAPL/FOE antigen affinity purified polyclonal antibody (Catalog # A03684-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAPL/FOE at approximately 180 kDa. The expected band size for WAPL/FOE is at 133 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03684-2-wapl-primary-antibodies-ihc-testing-2.jpgAnti-WAPL/FOE Antibody Picoband® IHC analysis of WAPL/FOE using anti-WAPL/FOE antibody (A03684-2). WAPL/FOE was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WAPL/FOE Antibody (A03684-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03684-2-wapl-primary-antibodies-ihc-testing-3.jpgAnti-WAPL/FOE Antibody Picoband® IHC analysis of WAPL/FOE using anti-WAPL/FOE antibody (A03684-2). WAPL/FOE was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WAPL/FOE Antibody (A03684-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03684-2-wapl-primary-antibodies-ihc-testing-4.jpgAnti-WAPL/FOE Antibody Picoband® IHC analysis of WAPL/FOE using anti-WAPL/FOE antibody (A03684-2). WAPL/FOE was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WAPL/FOE Antibody (A03684-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03684-2-wapl-primary-antibodies-ihc-testing-5.jpgAnti-WAPL/FOE Antibody Picoband® IHC analysis of WAPL/FOE using anti-WAPL/FOE antibody (A03684-2). WAPL/FOE was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WAPL/FOE Antibody (A03684-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03684-2-wapl-primary-antibodies-if-testing-6.jpgAnti-WAPL/FOE Antibody Picoband® IF analysis of WAPL/FOE using anti-WAPL/FOE antibody (A03684-2). WAPL/FOE was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WAPL/FOE Antibody (A03684-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03684-2-wapl-primary-antibodies-fcm-testing-7.pngAnti-WAPL/FOE Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-WAPL/FOE antibody (A03684-2). Overlay histogram showing A431 cells stained with A03684-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WAPL/FOE Antibody (A03684-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-znf609-picoband-trade-antibody-a15674-1-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15674-1-znf609-primary-antibodies-wb-testing-1.jpgAnti-ZNF609 Antibody Picoband® Western blot analysis of ZNF609 using anti-ZNF609 antibody (A15674-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF609 antigen affinity purified polyclonal antibody (Catalog # A15674-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNF609 at approximately 190 kDa. The expected band size for ZNF609 is at 151 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15674-1-znf609-primary-antibodies-fcm-testing-2.pngAnti-ZNF609 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-ZNF609 antibody (A15674-1). Overlay histogram showing Caco-2 cells stained with A15674-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZNF609 Antibody (A15674-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ghrelin-ghrl-picoband-trade-antibody-a01710-2-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01710-2-ghrl-primary-antibodies-ihc-testing-1.jpgAnti-Ghrelin/GHRL Antibody IHC analysis of Ghrelin/GHRL using anti-Ghrelin/GHRL antibody (A01710-2). Ghrelin/GHRL was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ghrelin/GHRL Antibody (A01710-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-zgc-153521-antibody-dz41186-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41186-zgc_153521-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish zgc: 153521 Antibody IHC analysis of Zgc: 153521 using anti-Zgc: 153521 antibody (DZ41186). Zgc: 153521 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Zgc: 153521 Antibody (DZ41186) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41186-zgc_153521-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish zgc: 153521 Antibody IHC analysis of Zgc: 153521 using anti-Zgc: 153521 antibody (DZ41186). Zgc: 153521 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Zgc: 153521 Antibody (DZ41186) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41186-zgc_153521-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish zgc: 153521 Antibody IHC analysis of Zgc: 153521 using anti-Zgc: 153521 antibody (DZ41186). Zgc: 153521 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Zgc: 153521 Antibody (DZ41186) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-clipa8-antibody-dz41194-boster.html2026-03-10T04:33:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-clipb17-antibody-dz41195-boster.html2026-03-10T04:33:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-coxiella-burnetii-cbu-1314-antibody-dz41196-boster.html2026-03-10T04:33:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-cuscuta-campestris-ccam-locus22811-antibody-dz41199-boster.html2026-03-10T04:33:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-strip-antibody-dz41200-boster.html2026-03-10T04:33:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-cont-antibody-dz41140-boster.html2026-03-10T04:33:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif5-picoband-trade-antibody-m04623-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04623-eif5-primary-antibodies-wb-testing-1.jpgAnti-EIF5 Antibody Picoband® (monoclonal, 2F13C4) Western blot analysis of EIF5 using anti-EIF5 antibody (M04623). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EIF5 antigen affinity purified monoclonal antibody (Catalog # M04623) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EIF5 at approximately 49 kDa. The expected band size for EIF5 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04623-eif5-primary-antibodies-fcm-testing-2.pngAnti-EIF5 Antibody Picoband® (monoclonal, 2F13C4) Flow Cytometry analysis of SiHa cells using anti-EIF5 antibody (M04623). Overlay histogram showing SiHa cells stained with M04623 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF5 Antibody (M04623, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04623-eif5-primary-antibodies-if-testing-3.jpgAnti-EIF5 Antibody Picoband® (monoclonal, 2F13C4) IF analysis of EIF5 using anti-EIF5 antibody (M04623). EIF5 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-EIF5 Antibody (M04623) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpp-picoband-trade-antibody-m01718-4-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01718-4-alpp-primary-antibodies-wb-testing-1.jpgAnti-ALPP Antibody Picoband® (monoclonal, 6C7G3) Western blot analysis of ALPP using anti-ALPP antibody (M01718-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ALPP antigen affinity purified monoclonal antibody (Catalog # M01718-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ALPP at approximately 70 kDa. The expected band size for ALPP is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01718-4-alpp-primary-antibodies-if-testing-2.jpgAnti-ALPP Antibody Picoband® (monoclonal, 6C7G3) IF analysis of ALPP using anti-ALPP antibody (M01718-4). ALPP was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-ALPP Antibody (M01718-4) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01718-4-alpp-primary-antibodies-ihc-testing-3.jpgAnti-ALPP Antibody Picoband® (monoclonal, 6C7G3) IHC analysis of ALPP using anti-ALPP antibody (M01718-4). ALPP was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ALPP Antibody (M01718-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01718-4-alpp-primary-antibodies-if-testing-4.jpgAnti-ALPP Antibody Picoband® (monoclonal, 6C7G3) IF analysis of ALPP using anti-ALPP antibody (M01718-4). ALPP was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-ALPP Antibody (M01718-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01718-4-alpp-primary-antibodies-fcm-testing-5.pngAnti-ALPP Antibody Picoband® (monoclonal, 6C7G3) Flow Cytometry analysis of SiHa cells using anti-ALPP antibody (M01718-4). Overlay histogram showing SiHa cells stained with M01718-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ALPP Antibody (M01718-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-msh2-picoband-trade-antibody-m00140-5-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-wb-testing-1.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) Western blot analysis of MSH2 using anti-MSH2 antibody (M00140-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human COLO-320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MSH2 antigen affinity purified monoclonal antibody (Catalog # M00140-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105 kDa. The expected band size for MSH2 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-ihc-testing-2.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) IHC analysis of MSH2 using anti-MSH2 antibody (M00140-5). MSH2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-ihc-testing-3.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) IHC analysis of MSH2 using anti-MSH2 antibody (M00140-5). MSH2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-ihc-testing-4.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) IHC analysis of MSH2 using anti-MSH2 antibody (M00140-5). MSH2 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-ihc-testing-5.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) IHC analysis of MSH2 using anti-MSH2 antibody (M00140-5). MSH2 was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-ihc-testing-6.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) IHC analysis of MSH2 using anti-MSH2 antibody (M00140-5). MSH2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-ihc-testing-7.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) IHC analysis of MSH2 using anti-MSH2 antibody (M00140-5). MSH2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00140-5-msh2-primary-antibodies-if-testing-8.jpgAnti-MSH2 Antibody Picoband® (monoclonal, 6B4F7) IF analysis of MSH2 using anti-MSH2 antibody (M00140-5). MSH2 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-MSH2 Antibody (M00140-5) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-shp1-ptpn6-picoband-trade-antibody-m00938-2-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00938-2-ptpn6-primary-antibodies-wb-testing-1.jpgAnti-SHP1/PTPN6 Antibody Picoband® (monoclonal, 8H11B10) Western blot analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (M00938-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SHP1/PTPN6 antigen affinity purified monoclonal antibody (Catalog # M00938-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SHP1/PTPN6 at approximately 68 kDa. The expected band size for SHP1/PTPN6 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00938-2-ptpn6-primary-antibodies-ihc-testing-2.jpgAnti-SHP1/PTPN6 Antibody Picoband® (monoclonal, 8H11B10) IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (M00938-2). SHP1/PTPN6 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SHP1/PTPN6 Antibody (M00938-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00938-2-ptpn6-primary-antibodies-ihc-testing-3.jpgAnti-SHP1/PTPN6 Antibody Picoband® (monoclonal, 8H11B10) IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (M00938-2). SHP1/PTPN6 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SHP1/PTPN6 Antibody (M00938-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00938-2-ptpn6-primary-antibodies-ihc-testing-4.jpgAnti-SHP1/PTPN6 Antibody Picoband® (monoclonal, 8H11B10) IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (M00938-2). SHP1/PTPN6 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SHP1/PTPN6 Antibody (M00938-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00938-2-ptpn6-primary-antibodies-ihc-testing-5.jpgAnti-SHP1/PTPN6 Antibody Picoband® (monoclonal, 8H11B10) IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (M00938-2). SHP1/PTPN6 was detected in a paraffin-embedded section of human SM fatty carcinoma of the left kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SHP1/PTPN6 Antibody (M00938-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00938-2-ptpn6-primary-antibodies-if-testing-6.jpgAnti-SHP1/PTPN6 Antibody Picoband® (monoclonal, 8H11B10) IF analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (M00938-2). SHP1/PTPN6 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-SHP1/PTPN6 Antibody (M00938-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00938-2-ptpn6-primary-antibodies-fcm-testing-7.pngAnti-SHP1/PTPN6 Antibody Picoband® (monoclonal, 8H11B10) Flow Cytometry analysis of Caco-2 cells using anti-SHP1/PTPN6 antibody (M00938-2). Overlay histogram showing Caco-2 cells stained with M00938-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SHP1/PTPN6 Antibody (M00938-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fen1-picoband-trade-antibody-m01484-3-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-wb-testing-1.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) Western blot analysis of FEN1 using anti-FEN1 antibody (M01484-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FEN1 antigen affinity purified monoclonal antibody (Catalog # M01484-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FEN1 at approximately 48 kDa. The expected band size for FEN1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-ihc-testing-2.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-ihc-testing-3.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-ihc-testing-4.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-ihc-testing-5.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-ihc-testing-6.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-ihc-testing-7.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-ihc-testing-8.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-if-testing-9.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) IF analysis of FEN1 using anti-FEN1 antibody (M01484-3). FEN1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-FEN1 Antibody (M01484-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-3-fen1-primary-antibodies-fcm-testing-10.pngAnti-FEN1 Antibody Picoband® (monoclonal, 6F3F2) Flow Cytometry analysis of A431 cells using anti-FEN1 antibody (M01484-3). Overlay histogram showing A431 cells stained with M01484-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-FEN1 Antibody (M01484-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fen1-picoband-trade-antibody-m01484-4-boster.html2026-03-17T05:12:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-wb-testing-1.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) Western blot analysis of FEN1 using anti-FEN1 antibody (M01484-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FEN1 antigen affinity purified monoclonal antibody (Catalog # M01484-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FEN1 at approximately 48 kDa. The expected band size for FEN1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-ihc-testing-2.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-4). FEN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-ihc-testing-3.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-4). FEN1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-ihc-testing-4.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-4). FEN1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-ihc-testing-5.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-4). FEN1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-ihc-testing-6.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-4). FEN1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-ihc-testing-7.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) IHC analysis of FEN1 using anti-FEN1 antibody (M01484-4). FEN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-FEN1 Antibody (M01484-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-if-testing-8.jpgAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) IF analysis of FEN1 using anti-FEN1 antibody (M01484-4). FEN1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-FEN1 Antibody (M01484-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01484-4-fen1-primary-antibodies-fcm-testing-9.pngAnti-FEN1 Antibody Picoband® (monoclonal, 7D11D7) Flow Cytometry analysis of A431 cells using anti-FEN1 antibody (M01484-4). Overlay histogram showing A431 cells stained with M01484-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-FEN1 Antibody (M01484-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-yy1-picoband-trade-antibody-m00833-1-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-1-yy1-primary-antibodies-wb-testing-1.jpgAnti-YY1 Antibody Picoband® (monoclonal, 6H3E1) Western blot analysis of YY1 using anti-YY1 antibody (M00833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human MDA-MB-453 lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-YY1 antigen affinity purified monoclonal antibody (Catalog # M00833-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-1-yy1-primary-antibodies-ihc-testing-2.jpgAnti-YY1 Antibody Picoband® (monoclonal, 6H3E1) IHC analysis of YY1 using anti-YY1 antibody (M00833-1). YY1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-1-yy1-primary-antibodies-fcm-testing-3.pngAnti-YY1 Antibody Picoband® (monoclonal, 6H3E1) Flow Cytometry analysis of PC-3 cells using anti-YY1 antibody (M00833-1). Overlay histogram showing PC-3 cells stained with M00833-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-YY1 Antibody (M00833-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-yy1-picoband-trade-antibody-m00833-2-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-2-yy1-primary-antibodies-wb-testing-1.jpgAnti-YY1 Antibody Picoband® (monoclonal, 2C10F9) Western blot analysis of YY1 using anti-YY1 antibody (M00833-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human MDA-MB-453 lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-YY1 antigen affinity purified monoclonal antibody (Catalog # M00833-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-2-yy1-primary-antibodies-ihc-testing-2.jpgAnti-YY1 Antibody Picoband® (monoclonal, 2C10F9) IHC analysis of YY1 using anti-YY1 antibody (M00833-2). YY1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-2-yy1-primary-antibodies-ihc-testing-3.jpgAnti-YY1 Antibody Picoband® (monoclonal, 2C10F9) IHC analysis of YY1 using anti-YY1 antibody (M00833-2). YY1 was detected in a paraffin-embedded section of human pancreatic ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-2-yy1-primary-antibodies-ihc-testing-4.jpgAnti-YY1 Antibody Picoband® (monoclonal, 2C10F9) IHC analysis of YY1 using anti-YY1 antibody (M00833-2). YY1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-2-yy1-primary-antibodies-ihc-testing-5.jpgAnti-YY1 Antibody Picoband® (monoclonal, 2C10F9) IHC analysis of YY1 using anti-YY1 antibody (M00833-2). YY1 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-2-yy1-primary-antibodies-fcm-testing-6.pngAnti-YY1 Antibody Picoband® (monoclonal, 2C10F9) Flow Cytometry analysis of PC-3 cells using anti-YY1 antibody (M00833-2). Overlay histogram showing PC-3 cells stained with M00833-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-YY1 Antibody (M00833-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-yy1-picoband-trade-antibody-m00833-3-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-wb-testing-1.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) Western blot analysis of YY1 using anti-YY1 antibody (M00833-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human MDA-MB-453 lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-YY1 antigen affinity purified monoclonal antibody (Catalog # M00833-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-ihc-testing-2.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) IHC analysis of YY1 using anti-YY1 antibody (M00833-3). YY1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-ihc-testing-3.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) IHC analysis of YY1 using anti-YY1 antibody (M00833-3). YY1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-ihc-testing-4.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) IHC analysis of YY1 using anti-YY1 antibody (M00833-3). YY1 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-ihc-testing-5.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) IHC analysis of YY1 using anti-YY1 antibody (M00833-3). YY1 was detected in a paraffin-embedded section of human pancreatic ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-ihc-testing-6.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) IHC analysis of YY1 using anti-YY1 antibody (M00833-3). YY1 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-ihc-testing-7.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) IHC analysis of YY1 using anti-YY1 antibody (M00833-3). YY1 was detected in a paraffin-embedded section of human the muscular layer of a colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YY1 Antibody (M00833-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-if-testing-8.jpgAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) IF analysis of YY1 using anti-YY1 antibody (M00179-1). YY1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-YY1 Antibody (M00179-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00833-3-yy1-primary-antibodies-fcm-testing-9.pngAnti-YY1 Antibody Picoband® (monoclonal, 3F3E7) Flow Cytometry analysis of A431 cells using anti-YY1 antibody (M00833-3). Overlay histogram showing A431 cells stained with M00833-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-YY1 Antibody (M00833-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hgs-picoband-trade-antibody-m01174-1-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-wb-testing-1.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) Western blot analysis of HGS using anti-HGS antibody (M00179-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HGS antigen affinity purified monoclonal antibody (Catalog # M00179-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HGS at approximately 110 kDa. The expected band size for HGS is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-ihc-testing-2.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) IHC analysis of HGS using anti-HGS antibody (M01174-1). HGS was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-ihc-testing-3.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) IHC analysis of HGS using anti-HGS antibody (M01174-1). HGS was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-ihc-testing-4.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) IHC analysis of HGS using anti-HGS antibody (M01174-1). HGS was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-ihc-testing-5.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) IHC analysis of HGS using anti-HGS antibody (M01174-1). HGS was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-ihc-testing-6.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) IHC analysis of HGS using anti-HGS antibody (M01174-1). HGS was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-ihc-testing-7.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) IHC analysis of HGS using anti-HGS antibody (M01174-1). HGS was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-ihc-testing-8.jpgAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) IHC analysis of HGS using anti-HGS antibody (M01174-1). HGS was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-1-hgs-primary-antibodies-fcm-testing-9.pngAnti-HGS Antibody Picoband® (monoclonal, 6C2E1) Flow Cytometry analysis of Caco-2 cells using anti-HGS antibody (M01174-1). Overlay histogram showing Caco-2 cells stained with M01174-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HGS Antibody (M01174-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hgs-picoband-trade-antibody-m01174-2-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-wb-testing-1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) Western blot analysis of HGS using anti-HGS antibody (M01174-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HGS antigen affinity purified monoclonal antibody (Catalog # M01174-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HGS at approximately 110 kDa. The expected band size for HGS is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-ihc-testing-2_1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IHC analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-fcm-testing-10_1.pngAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) Flow Cytometry analysis of Caco-2 cells using anti-HGS antibody (M01174-2). Overlay histogram showing Caco-2 cells stained with M01174-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HGS Antibody (M01174-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-ihc-testing-3_1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IHC analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-ihc-testing-4_1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IHC analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-ihc-testing-5.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IHC analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-ihc-testing-6_1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IHC analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-ihc-testing-7_1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IHC analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-ihc-testing-8_1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IHC analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGS Antibody (M01174-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01174-2-hgs-primary-antibodies-if-testing-9_1.jpgAnti-HGS Antibody Picoband® (monoclonal, 4B7E2) IF analysis of HGS using anti-HGS antibody (M01174-2). HGS was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-HGS Antibody (M01174-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ywhae-picoband-trade-antibody-m01687-2-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-wb-testing-1.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) Western blot analysis of YWHAE using anti-YWHAE antibody (M01687-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-YWHAE antigen affinity purified monoclonal antibody (Catalog # M01687-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for YWHAE at approximately 29 kDa. The expected band size for YWHAE is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-2.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-3.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-4.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-5.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-6.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-7.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-8.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-if-testing-9.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IF analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-fcm-testing-10.pngAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) Flow Cytometry analysis of ANA-1 cells using anti-YWHAE antibody (M01687-2). Overlay histogram showing ANA-1 cells stained with M01687-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-YWHAE Antibody (M01687-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-fcm-testing-11.pngAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) Flow Cytometry analysis of NRK cells using anti-YWHAE antibody (M01687-2). Overlay histogram showing NRK cells stained with M01687-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-YWHAE Antibody (M01687-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01687-2-ywhae-primary-antibodies-ihc-testing-12.jpgAnti-YWHAE Antibody Picoband® (monoclonal, 3G11G2) IHC analysis of YWHAE using anti-YWHAE antibody (M01687-2). YWHAE was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-YWHAE Antibody (M01687-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pcbp2-hnrnp-e2-picoband-trade-antibody-m02425-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-pcbp2-primary-antibodies-wb-testing-1.jpgAnti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7) Western blot analysis of PCBP2/hnRNP E2 using anti-PCBP2/hnRNP E2 antibody (M02425). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PCBP2/hnRNP E2 antigen affinity purified monoclonal antibody (Catalog # M02425) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCBP2/hnRNP E2 at approximately 39 kDa. The expected band size for PCBP2/hnRNP E2 is at 39 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-pcbp2-primary-antibodies-if-testing-2.jpgAnti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7) IF analysis of PCBP2/hnRNP E2 using anti-PCBP2/hnRNP E2 antibody (M02425). PCBP2/hnRNP E2 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-PCBP2/hnRNP E2 Antibody (M02425) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-pcbp2-primary-antibodies-fcm-testing-3.pngAnti-PCBP2/hnRNP E2 Antibody Picoband® (monoclonal, 4B9C7) Flow Cytometry analysis of PC-3 cells using anti-PCBP2/hnRNP E2 antibody (M02425). Overlay histogram showing PC-3 cells stained with M02425 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PCBP2/hnRNP E2 Antibody (M02425, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sorbitol-dehydrogenase-sord-picoband-trade-antibody-m07851-1-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07851-1-sord-primary-antibodies-wb-testing-1.jpgAnti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) Western blot analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (M07851-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Sorbitol Dehydrogenase/SORD antigen affinity purified monoclonal antibody (Catalog # M07851-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/SORD at approximately 40 kDa. The expected band size for Sorbitol Dehydrogenase/SORD is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07851-1-sord-primary-antibodies-ihc-testing-2.jpgAnti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (M07851-1). Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (M07851-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07851-1-sord-primary-antibodies-ihc-testing-3.jpgAnti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (M07851-1). Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (M07851-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07851-1-sord-primary-antibodies-ihc-testing-4.jpgAnti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (M07851-1). Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human SM fatty carcinoma of the left kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (M07851-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07851-1-sord-primary-antibodies-ihc-testing-5.jpgAnti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (M07851-1). Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (M07851-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07851-1-sord-primary-antibodies-fcm-testing-6.pngAnti-Sorbitol Dehydrogenase/SORD Antibody Picoband® (monoclonal, 12B10G2) Flow Cytometry analysis of U20S cells using anti-Sorbitol Dehydrogenase/SORD antibody (M07851-1). Overlay histogram showing U20S cells stained with M07851-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Sorbitol Dehydrogenase/SORD Antibody (M07851-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cdk2-picoband-trade-antibody-m00166-3-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-3-cdk2-primary-antibodies-wb-testing-1.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) Western blot analysis of Cdk2 using anti-Cdk2 antibody (M00166-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat L6 whole cell lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cdk2 antigen affinity purified monoclonal antibody (Catalog # M00166-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cdk2 at approximately 30 kDa. The expected band size for Cdk2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-3-cdk2-primary-antibodies-ihc-testing-2.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-3). Cdk2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-3-cdk2-primary-antibodies-ihc-testing-3.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-3). Cdk2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-3-cdk2-primary-antibodies-ihc-testing-4.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-3). Cdk2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-3-cdk2-primary-antibodies-fcm-testing-5.pngAnti-Cdk2 Antibody Picoband® (monoclonal, 5B12D1) Flow Cytometry analysis of ANA-1 cells using anti-Cdk2 antibody (M00166-3). Overlay histogram showing ANA-1 cells stained with M00166-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cdk2 Antibody (M00166-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cdk2-picoband-trade-antibody-m00166-4-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-4-cdk2-primary-antibodies-wb-testing-1.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) Western blot analysis of Cdk2 using anti-Cdk2 antibody (M00166-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat L6 whole cell lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cdk2 antigen affinity purified monoclonal antibody (Catalog # M00166-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cdk2 at approximately 30 kDa. The expected band size for Cdk2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-4-cdk2-primary-antibodies-ihc-testing-2.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-4). Cdk2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-4-cdk2-primary-antibodies-ihc-testing-3.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-4). Cdk2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-4-cdk2-primary-antibodies-ihc-testing-4.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-4). Cdk2 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-4-cdk2-primary-antibodies-ihc-testing-5.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-4). Cdk2 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-4-cdk2-primary-antibodies-ihc-testing-6.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-4). Cdk2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-4-cdk2-primary-antibodies-ihc-testing-7.jpgAnti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-4). Cdk2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-eif3e-picoband-trade-antibody-m00481-1-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00481-1-eif3e-primary-antibodies-wb-testing-1.jpgAnti-EIF3e Antibody Picoband® (monoclonal, 10F5H6) Western blot analysis of EIF3E using anti-EIF3E antibody (M00481-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse ANA-1 whole cell lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EIF3E antigen affinity purified monoclonal antibody (Catalog # M00481-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for EIF3E at approximately 52 kDa. The expected band size for EIF3E is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00481-1-eif3e-primary-antibodies-fcm-testing-2.pngAnti-EIF3e Antibody Picoband® (monoclonal, 10F5H6) Flow Cytometry analysis of U20S cells using anti-EIF3E antibody (M00481-1). Overlay histogram showing U20S cells stained with M00481-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF3E Antibody (M00481-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-shc-picoband-trade-antibody-m00796-3-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00796-3-shc1-primary-antibodies-wb-testing-1.jpgAnti-SHC Antibody Picoband® (monoclonal, 6D6E1) Western blot analysis of SHC1 using anti-SHC1 antibody (M00796-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SHC1 antigen affinity purified monoclonal antibody (Catalog # M00796-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SHC1 at approximately 65 kDa. The expected band size for SHC1 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cd147-emmprin-picoband-trade-antibody-m00248-4-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-wb-testing-1.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD147/Emmprin antigen affinity purified monoclonal antibody (Catalog # M00248-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 35-60 kDa. The expected band size for CD147/Emmprin is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-ihc-testing-2.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-ihc-testing-3.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-ihc-testing-4.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of human ahepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-ihc-testing-5.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-ihc-testing-6.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-ihc-testing-7.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-ihc-testing-8.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-4-cd147-primary-antibodies-if-testing-9.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 3B13G7) IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-4). CD147/Emmprin was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-CD147/Emmprin Antibody (M00248-4) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cd147-emmprin-picoband-trade-antibody-m00248-5-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-wb-testing-1.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD147/Emmprin antigen affinity purified monoclonal antibody (Catalog # M00248-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 35-60 kDa. The expected band size for CD147/Emmprin is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-ihc-testing-2.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-ihc-testing-3.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-ihc-testing-4.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-ihc-testing-5.pngAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-ihc-testing-6.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-ihc-testing-7.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-ihc-testing-8.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-if-testing-9.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-5). CD147/Emmprin was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-CD147/Emmprin Antibody (M00248-5) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-5-cd147-primary-antibodies-fcm-testing-10.pngAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 7H5E7) Flow Cytometry analysis of SiHa cells using anti-CD147/Emmprin antibody (M00248-5). Overlay histogram showing SiHa cells stained with M00248-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD147/Emmprin Antibody (M00248-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd147-emmprin-picoband-trade-antibody-m00248-6-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-wb-testing-1.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD147/Emmprin antigen affinity purified monoclonal antibody (Catalog # M00248-6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 35-60 kDa. The expected band size for CD147/Emmprin is at 42 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-ihc-testing-2.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). CD147/Emmprin was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-ihc-testing-3.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). CD147/Emmprin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-ihc-testing-4.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). CD147/Emmprin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-ihc-testing-5.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). CD147/Emmprin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-ihc-testing-6.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). CD147/Emmprin was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-ihc-testing-7.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). CD147/Emmprin was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD147/Emmprin Antibody (M00248-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-6-cd147-primary-antibodies-if-testing-8.jpgAnti-CD147/Emmprin Antibody Picoband® (monoclonal, 6H2B2) IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (M00248-6). CD147/Emmprin was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-CD147/Emmprin Antibody (M00248-6) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdia6-picoband-trade-antibody-m03813-1-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-wb-testing-1.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) Western blot analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HT1080 lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDIA6 antigen affinity purified monoclonal antibody (Catalog # M03813-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDIA6 at approximately 48 kDa. The expected band size for PDIA6 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-2.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-3.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-4.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-5.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-6.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-7.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-8.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-ihc-testing-9.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IHC analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-if-testing-10.jpgAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) IF analysis of PDIA6 using anti-PDIA6 antibody (M03813-1). PDIA6 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-PDIA6 Antibody (M03813-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03813-1-pdia6-primary-antibodies-fcm-testing-11.pngAnti-PDIA6 Antibody Picoband® (monoclonal, 3H5E7) Flow Cytometry analysis of SiHa cells using anti-PDIA6 antibody (M03813-1). Overlay histogram showing SiHa cells stained with M03813-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PDIA6 Antibody (M03813-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/picokine-elisa-kits/human-ceacam3-picokine-elisa-kit-ek2163-boster.html2026-03-10T04:33:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2163.pngHuman CEACAM3 ELISA Kit PicoKine®Human CEACAM3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cfhr1-picokine-elisa-kit-ek2165-boster.html2026-03-10T04:33:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2165.jpgHuman CFHR1 ELISA Kit PicoKine®Human CFHR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cfhr4-picokine-elisa-kit-ek2166-boster.html2026-03-10T04:33:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2166.jpgHuman CFHR4 ELISA Kit PicoKine®Human CFHR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-chondroadherin-picokine-elisa-kit-ek2167-boster.html2026-03-10T04:33:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2167.jpgHuman Chondroadherin ELISA Kit PicoKine®Human Chondroadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-chrdl2-picokine-elisa-kit-ek2168-boster.html2026-03-10T04:33:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2168.jpgHuman CHRDL2 ELISA Kit PicoKine®Human CHRDL2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-acadm-mcad-picoband-trade-antibody-a02383-3-boster.html2026-03-17T05:12:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02383-3-acadm-primary-antibodies-wb-testing-1.jpgAnti-ACADM/MCAD Antibody Picoband® Western blot analysis of ACADM/MCAD using anti-ACADM/MCAD antibody (A02383-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse heart tissue lysates, Lane 10: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM/MCAD antigen affinity purified polyclonal antibody (Catalog # A02383-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADM/MCAD at approximately 47 kDa. The expected band size for ACADM/MCAD is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02383-3-acadm-primary-antibodies-ihc-testing-2.jpgAnti-ACADM/MCAD Antibody Picoband® IHC analysis of ACADM/MCAD using anti-ACADM/MCAD antibody (A02383-3). ACADM/MCAD was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACADM/MCAD Antibody (A02383-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02383-3-acadm-primary-antibodies-ihc-testing-3.jpgAnti-ACADM/MCAD Antibody Picoband® IHC analysis of ACADM/MCAD using anti-ACADM/MCAD antibody (A02383-3). ACADM/MCAD was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACADM/MCAD Antibody (A02383-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02383-3-acadm-primary-antibodies-if-testing-4.jpgAnti-ACADM/MCAD Antibody Picoband® IF analysis of ACADM/MCAD using anti-ACADM/MCAD antibody (A02383-3). ACADM/MCAD was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ACADM/MCAD Antibody (A02383-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02383-3-acadm-primary-antibodies-fcm-testing-5.pngAnti-ACADM/MCAD Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-ACADM/MCAD antibody (A02383-3). Overlay histogram showing Daudi cells stained with A02383-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACADM/MCAD Antibody (A02383-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-acat2-picoband-trade-antibody-a03245-1-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03245-1-acat2-primary-antibodies-wb-testing-1_1.jpgAnti-ACAT2 Antibody Picoband® Western blot analysis of ACAT2 using anti-ACAT2 antibody (A03245-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT2 antigen affinity purified polyclonal antibody (Catalog # A03245-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for ACAT2 at approximately 41 kDa. The expected band size for ACAT2 is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03245-1-acat2-primary-antibodies-fcm-testing-2.pngAnti-ACAT2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-ACAT2 antibody (A03245-1). Overlay histogram showing HepG2 cells stained with A03245-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACAT2 Antibody (A03245-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-acp2-picoband-trade-antibody-a06554-1-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06554-1-acp2-primary-antibodies-wb-testing-1.jpgAnti-ACP2 Antibody Picoband® Western blot analysis of ACP2 using anti-ACP2 antibody (A06554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACP2 antigen affinity purified polyclonal antibody (Catalog # A06554-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACP2 at approximately 76 kDa. The expected band size for ACP2 is at 76 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-facl4-acsl4-picoband-trade-antibody-a04372-3-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04372-3-acsl4-primary-antibodies-wb-testing-1.jpgAnti-FACL4/ACSL4 Antibody Picoband® Western blot analysis of FACL4/ACSL4 using anti-FACL4/ACSL4 antibody (A04372-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat lung tissue lysates, Lane 9: rat stomach tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse stomach tissue lysates, Lane 14: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FACL4/ACSL4 antigen affinity purified polyclonal antibody (Catalog # A04372-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FACL4/ACSL4 at approximately 79 kDa. The expected band size for FACL4/ACSL4 is at 79 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-adamts13-picoband-trade-antibody-a00586-1-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00586-1-adamts13-primary-antibodies-wb-testing-1.jpgAnti-ADAMTS13 Antibody Picoband® Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody (A00586-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS13 antigen affinity purified polyclonal antibody (Catalog # A00586-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS13 at approximately 154 kDa. The expected band size for ADAMTS13 is at 154 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-angiotensinogen-agt-picoband-trade-antibody-a02103-3-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02103-3-agt-primary-antibodies-wb-testing-1.jpgAnti-Angiotensinogen/AGT Antibody Picoband® Western blot analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody (A02103-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiotensinogen/AGT antigen affinity purified polyclonal antibody (Catalog # A02103-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiotensinogen/AGT at approximately 55 kDa. The expected band size for Angiotensinogen/AGT is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02103-3-agt-primary-antibodies-ihc-testing-2.jpgAnti-Angiotensinogen/AGT Antibody Picoband® IHC analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody (A02103-3). Angiotensinogen/AGT was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Angiotensinogen/AGT Antibody (A02103-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02103-3-agt-primary-antibodies-fcm-testing-3.pngAnti-Angiotensinogen/AGT Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Angiotensinogen/AGT antibody (A02103-3). Overlay histogram showing HepG2 cells stained with A02103-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Angiotensinogen/AGT Antibody (A02103-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aha1-ahsa1-picoband-trade-antibody-a05733-2-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-wb-testing-1.jpgAnti-AHA1/AHSA1 Antibody Picoband® Western blot analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human MOLT4 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHA1/AHSA1 antigen affinity purified polyclonal antibody (Catalog # A05733-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHA1/AHSA1 at approximately 40 kDa. The expected band size for AHA1/AHSA1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-ihc-testing-2.jpgAnti-AHA1/AHSA1 Antibody Picoband® IHC analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-ihc-testing-3.jpgAnti-AHA1/AHSA1 Antibody Picoband® IHC analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-ihc-testing-4.jpgAnti-AHA1/AHSA1 Antibody Picoband® IHC analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-ihc-testing-5.jpgAnti-AHA1/AHSA1 Antibody Picoband® IHC analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-ihc-testing-6.jpgAnti-AHA1/AHSA1 Antibody Picoband® IHC analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-ihc-testing-7.jpgAnti-AHA1/AHSA1 Antibody Picoband® IHC analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-ihc-testing-8.jpgAnti-AHA1/AHSA1 Antibody Picoband® IHC analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-if-testing-9.jpgAnti-AHA1/AHSA1 Antibody Picoband® IF analysis of AHA1/AHSA1 using anti-AHA1/AHSA1 antibody (A05733-2). AHA1/AHSA1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AHA1/AHSA1 Antibody (A05733-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05733-2-ahsa1-primary-antibodies-fcm-testing-10.pngAnti-AHA1/AHSA1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-AHA1/AHSA1 antibody (A05733-2). Overlay histogram showing HepG2 cells stained with A05733-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHA1/AHSA1 Antibody (A05733-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aldh3a1-picoband-trade-antibody-a01121-4-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01121-4-aldh3a1-primary-antibodies-wb-testing-1.jpgAnti-ALDH3A1 Antibody Picoband® Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # A01121-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01121-4-aldh3a1-primary-antibodies-ihc-testing-2.jpgAnti-ALDH3A1 Antibody Picoband® IHC analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-4). ALDH3A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH3A1 Antibody (A01121-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01121-4-aldh3a1-primary-antibodies-if-testing-3.jpgAnti-ALDH3A1 Antibody Picoband® IF analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-4). ALDH3A1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALDH3A1 Antibody (A01121-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-apelin-apln-picoband-trade-antibody-a02075-1-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02075-1-apln-primary-antibodies-ihc-testing-1.jpgAnti-Apelin/APLN Antibody IHC analysis of Apelin/APLN using anti-Apelin/APLN antibody (A02075-1). Apelin/APLN was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Apelin/APLN Antibody (A02075-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02075-1-apln-primary-antibodies-ihc-testing-2.jpgAnti-Apelin/APLN Antibody IHC analysis of Apelin/APLN using anti-Apelin/APLN antibody (A02075-1). Apelin/APLN was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Apelin/APLN Antibody (A02075-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02075-1-apln-primary-antibodies-ihc-testing-3.jpgAnti-Apelin/APLN Antibody IHC analysis of Apelin/APLN using anti-Apelin/APLN antibody (A02075-1). Apelin/APLN was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Apelin/APLN Antibody (A02075-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02075-1-apln-primary-antibodies-ihc-testing-4.jpgAnti-Apelin/APLN Antibody IHC analysis of Apelin/APLN using anti-Apelin/APLN antibody (A02075-1). Apelin/APLN was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Apelin/APLN Antibody (A02075-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-aquaporin-4-aqp4-picoband-trade-antibody-a01049-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01049-aqp4-primary-antibodies-ihc-testing-4.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband®IHC analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Aquaporin 4/AQP4 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4/AQP4 Antibody (A01049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01049-aqp4-primary-antibodies-wb-testing-1.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband® Western blot analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4/AQP4 antigen affinity purified polyclonal antibody (Catalog # A01049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 4/AQP4 at approximately 32 kDa. The expected band size for Aquaporin 4/AQP4 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01049-aqp4-primary-antibodies-ihc-testing-2.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband® IHC analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Aquaporin 4/AQP4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4/AQP4 Antibody (A01049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01049-aqp4-primary-antibodies-wb-testing-2.pngAnti-Aquaporin 4/AQP4 Antibody Picoband® Western blot analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Normal group-rat colon tissue lysates, Lane 2: Model group-rat colon tissue lysates, Lane 3: Triditional Chinese medicine group-rat colon tissue lysates, Lane 4: Western medicine group-rat colon tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4/AQP4 antigen affinity purified polyclonal antibody (Catalog # A01049) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Aquaporin 4/AQP4 at approximately 37 kDa. The expected band size for Aquaporin 4/AQP4 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01049-aqp4-primary-antibodies-ihc-testing-3.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband® IHC analysis of Aquaporin 4/AQP4 using anti-Aquaporin 4/AQP4 antibody (A01049). Aquaporin 4/AQP4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4/AQP4 Antibody (A01049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01049-aqp4-primary-antibodies-fcm-testing-4.pngAnti-Aquaporin 4/AQP4 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-Aquaporin 4/AQP4 antibody (A01049). Overlay histogram showing A431 cells stained with A01049 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 4/AQP4 Antibody (A01049, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aqp11-picoband-trade-antibody-a11399-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11399-aqp11-primary-antibodies-wb-testing-1.jpgAnti-AQP11 Antibody Picoband® Western blot analysis of AQP11 using anti-AQP11 antibody (A11399). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP11 antigen affinity purified polyclonal antibody (Catalog # A11399) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP11 at approximately 53 kDa. The expected band size for AQP11 is at 53 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11399-aqp11-primary-antibodies-fcm-testing-2.pngAnti-AQP11 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-AQP11 antibody (A11399). Overlay histogram showing Caco-2 cells stained with A11399 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP11 Antibody (A11399, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atg4a-picoband-trade-antibody-a06539-3-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06539-3-atg4a-primary-antibodies-wb-testing-1.jpgAnti-ATG4A Antibody Picoband® Western blot analysis of ATG4A using anti-ATG4A antibody (A06539-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat L6 whole cell lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4A antigen affinity purified polyclonal antibody (Catalog # A06539-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4A at approximately 45-50 kDa. The expected band size for ATG4A is at 45-50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06539-3-atg4a-primary-antibodies-fcm-testing-2.pngAnti-ATG4A Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-ATG4A antibody (A06539-3). Overlay histogram showing K562 cells stained with A06539-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4A Antibody (A06539-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-batf2-picoband-trade-antibody-a09891-2-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09891-2-batf2-primary-antibodies-wb-testing-1.jpgAnti-Batf2 Antibody Picoband® Western blot analysis of Batf2 using anti-Batf2 antibody (A09891-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Batf2 antigen affinity purified polyclonal antibody (Catalog # A09891-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Batf2 at approximately 25 kDa. The expected band size for Batf2 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09891-2-batf2-primary-antibodies-ihc-testing-2.jpgAnti-Batf2 Antibody Picoband® IHC analysis of Batf2 using anti-Batf2 antibody (A09891-2). Batf2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Batf2 Antibody (A09891-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09891-2-batf2-primary-antibodies-ihc-testing-3.jpgAnti-Batf2 Antibody Picoband® IHC analysis of Batf2 using anti-Batf2 antibody (A09891-2). Batf2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Batf2 Antibody (A09891-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-caspase-8-casp8-picoband-trade-antibody-a00042-3-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-3-casp8-primary-antibodies-wb-testing-1.jpgAnti-Caspase-8/CASP8 Antibody Picoband® Western blot analysis of Caspase-8/CASP8 using anti-Caspase-8/CASP8 antibody (A00042-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-8/CASP8 antigen affinity purified polyclonal antibody (Catalog # A00042-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-8/CASP8 at approximately 55 kDa. The expected band size for Caspase-8/CASP8 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-3-casp8-primary-antibodies-fcm-testing-2.pngAnti-Caspase-8/CASP8 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-Caspase-8/CASP8 antibody (A00042-3). Overlay histogram showing K562 cells stained with A00042-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-8/CASP8 Antibody (A00042-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-caspase-9-casp9-picoband-trade-antibody-a00080-8-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-8-casp9-primary-antibodies-wb-testing-1.jpgAnti-Caspase-9/CASP9 Antibody Picoband® Western blot analysis of Caspase-9/CASP9 using anti-Caspase-9/CASP9 antibody (A00080-8). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-9/CASP9 antigen affinity purified polyclonal antibody (Catalog # A00080-8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-9/CASP9 at approximately 46 kDa. The expected band size for Caspase-9/CASP9 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-8-41598_2018_33552_fig8_html.pngAnti-Caspase-9/CASP9 Antibody Picoband®Effect of STS on intrinsic apoptosis-related factors in ARPE-19 cells under oxidative stress. ( a ) The representative protein gel blots of BAX, caspase-9, caspase-3 and BCL-2 are shown. Actin was utilized as a loading control. ( b – e ) The accompanying histogram of densitometric quantification of BAX, caspase-9, caspase-3 and BCL-2. Untreated cells were used as a positive control. ( f ) The effect of STS on MMP using the JC-1 dye in ARPE-19 cells under oxidative stress. The red/green fluorescent intensity ratio reflects MMP. ( g ) The accompanied histogram of MMP. The experiment was repeated 3 times in each group. Data were analyzed using one-way ANOVA and differences between measures were statistically significant when P < 0.05, Bonferroni correction of P-value was applied in multiple comparison. The statistical results show that there is no statistical difference between control and H 2 O 2 + 3MA, or H 2 O 2 + STS and H 2 O 2 + STS + 3MA in ( g ), the other groups showed statistically significant differences. Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-018-33552-2'>30310136</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-8-casp9-primary-antibodies-fcm-testing-2.pngAnti-Caspase-9/CASP9 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-Caspase-9/CASP9 antibody (A00080-8). Overlay histogram showing Caco-2 cells stained with A00080-8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9/CASP9 Antibody (A00080-8, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00080-8-casp9-primary-antibodies-fcm-testing-3.pngAnti-Caspase-9/CASP9 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-Caspase-9/CASP9 antibody (A00080-8). Overlay histogram showing K562 cells stained with A00080-8 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9/CASP9 Antibody (A00080-8, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cbl-picoband-trade-antibody-a00152-2-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00152-2-cbl-primary-antibodies-wb-testing-1.jpgAnti-CBL Antibody Picoband® Western blot analysis of CBL using anti-CBL antibody (A00152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human CCRF-CEM whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human Hela whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBL antigen affinity purified polyclonal antibody (Catalog # A00152-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBL at approximately 120 kDa. The expected band size for CBL is at 120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00152-2-cbl-primary-antibodies-ihc-testing-2.jpgAnti-CBL Antibody Picoband® IHC analysis of CBL using anti-CBL antibody (A00152-2). CBL was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBL Antibody (A00152-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00152-2-cbl-primary-antibodies-fcm-testing-3.pngAnti-CBL Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-CBL antibody (A00152-2). Overlay histogram showing U87 cells stained with A00152-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBL Antibody (A00152-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ccl21a-picoband-trade-antibody-a33349-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a33349-ccl21a-primary-antibodies-ihc-testing-1.jpgAnti-Ccl21a Antibody IHC analysis of Ccl21a using anti-Ccl21a antibody (A33349). Ccl21a was detected in a paraffin-embedded section of mouse lung tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl21a Antibody (A33349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a33349-ccl21a-primary-antibodies-ihc-testing-2.jpgAnti-Ccl21a Antibody IHC analysis of Ccl21a using anti-Ccl21a antibody (A33349). Ccl21a was detected in a paraffin-embedded section of rat lung tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl21a Antibody (A33349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cd6-picoband-trade-antibody-a03913-2-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03913-2-cd6-primary-antibodies-ihc-testing-1.jpgAnti-Cd6 Antibody IHC analysis of Cd6 using anti-Cd6 antibody (A03913-2). Cd6 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd6 Antibody (A03913-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cd7-picoband-trade-antibody-a01974-3-boster.html2026-03-17T05:12:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01974-3-cd7-primary-antibodies-wb-testing-1.jpgAnti-Cd7 Antibody Picoband® Western blot analysis of Cd7 using anti-Cd7 antibody (A01974-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd7 antigen affinity purified polyclonal antibody (Catalog # A01974-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd7 at approximately 36 kDa. The expected band size for Cd7 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cd70-picoband-trade-antibody-a02853-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02853-2-cd70-primary-antibodies-wb-testing-1.jpgAnti-Cd70 Antibody Picoband® Western blot analysis of Cd70 using anti-Cd70 antibody (A02853-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse B16 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd70 antigen affinity purified polyclonal antibody (Catalog # A02853-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd70 at approximately 30 kDa. The expected band size for Cd70 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02853-2-41598_2025_88671_fig9_html.pngAnti-Cd70 Antibody Picoband®Relative expression of genes expression in GBM tissues. Expression levels of WTAP and ZC3H13 genes were compared with those of the control group on GBM tissues, 10 GBM and adjacent tissues, respectively ( A ). The expression levels of siRNA WTAP and overexpression ZC3H13 genes ( B and C ). The expression levels of CD27, CD70, CD80, CD86, ICOS, CTLA4, and LAG3 in siRNA WTAP and overexpression ZC3H13 were demonstrated in Fig. D and F, respectively. The result were determined by qRT-PCR. The data are mean ± standard deviation of six replicate experiments. * P < 0.05, **P < 0.01 and ***P < 0.001 compared with the corresponding control values. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-88671-4'>39910141</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02853-2-cd70-primary-antibodies-wb-testing-2.jpgAnti-Cd70 Antibody Picoband® Western blot analysis of Cd70 using anti-Cd70 antibody (A02853-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse ANA-1 whole cell lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd70 antigen affinity purified polyclonal antibody (Catalog # A02853-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd70 at approximately 30 kDa. The expected band size for Cd70 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02853-2-cd70-primary-antibodies-ihc-testing-3.jpgAnti-Cd70 Antibody Picoband® IHC analysis of Cd70 using anti-Cd70 antibody (A02853-2). Cd70 was detected in a paraffin-embedded section of mouse lymphonode tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd70 Antibody (A02853-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02853-2-cd70-primary-antibodies-ihc-testing-4.jpgAnti-Cd70 Antibody Picoband® IHC analysis of Cd70 using anti-Cd70 antibody (A02853-2). Cd70 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd70 Antibody (A02853-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cox-iv-cox4i1-picoband-trade-antibody-a05442-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-2-cox4i1-primary-antibodies-wb-testing-1_1.jpgAnti-COX IV/COX4I1 Antibody Picoband® Western blot analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (A05442-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates. Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV/COX4I1 antigen affinity purified polyclonal antibody (Catalog # A05442-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX IV/COX4I1 at approximately 17 kDa. The expected band size for COX IV/COX4I1 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-2-cox4i1-primary-antibodies-ihc-testing-3.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (A05442-2). COX IV/COX4I1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX IV/COX4I1 Antibody (A05442-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-2-cox4i1-primary-antibodies-ihc-testing-2.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (A05442-2). COX IV/COX4I1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX IV/COX4I1 Antibody (A05442-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-2-cox4i1-primary-antibodies-ihc-testing-4.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (A05442-2). COX IV/COX4I1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX IV/COX4I1 Antibody (A05442-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-2-cox4i1-primary-antibodies-ihc-testing-5.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (A05442-2). COX IV/COX4I1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX IV/COX4I1 Antibody (A05442-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-2-cox4i1-primary-antibodies-ihc-testing-6.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (A05442-2). COX IV/COX4I1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX IV/COX4I1 Antibody (A05442-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05442-2-cox4i1-primary-antibodies-ihc-testing-7.jpgAnti-COX IV/COX4I1 Antibody Picoband® IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (A05442-2). COX IV/COX4I1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX IV/COX4I1 Antibody (A05442-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cx3cr1-picoband-trade-antibody-a00280-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00280-2-cx3cr1-primary-antibodies-ihc-testing-1.jpgAnti-CX3CR1 Antibody IHC analysis of CX3CR1 using anti-CX3CR1 antibody (A00280-2). CX3CR1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CX3CR1 Antibody (A00280-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00280-2-cx3cr1-primary-antibodies-ihc-testing-2.jpgAnti-CX3CR1 Antibody IHC analysis of CX3CR1 using anti-CX3CR1 antibody (A00280-2). CX3CR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CX3CR1 Antibody (A00280-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00280-2-cx3cr1-primary-antibodies-ihc-testing-3.jpgAnti-CX3CR1 Antibody IHC analysis of CX3CR1 using anti-CX3CR1 antibody (A00280-2). CX3CR1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CX3CR1 Antibody (A00280-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00280-2-cx3cr1-primary-antibodies-fcm-testing-4.pngAnti-CX3CR1 Antibody Flow Cytometry analysis of HEL cells using anti-CX3CR1 antibody (A00280-2). Overlay histogram showing HEL cells stained with A00280-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CX3CR1 Antibody (A00280-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dab2-picoband-trade-antibody-a01546-3-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01546-3-dab2-primary-antibodies-wb-testing-1.jpgAnti-DAB2 Antibody Picoband® Western blot analysis of DAB2 using anti-DAB2 antibody (A01546-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAB2 antigen affinity purified polyclonal antibody (Catalog # A01546-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAB2 at approximately 95 kDa. The expected band size for DAB2 is at 95 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01546-3-dab2-primary-antibodies-ihc-testing-2.jpgAnti-DAB2 Antibody Picoband® IHC analysis of DAB2 using anti-DAB2 antibody (A01546-3). DAB2 was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAB2 Antibody (A01546-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01546-3-dab2-primary-antibodies-if-testing-3.jpgAnti-DAB2 Antibody Picoband® IF analysis of DAB2 using anti-DAB2 antibody (A01546-3). DAB2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DAB2 Antibody (A01546-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01546-3-dab2-primary-antibodies-fcm-testing-4.pngAnti-DAB2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-DAB2 antibody (A01546-3). Overlay histogram showing HepG2 cells stained with A01546-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAB2 Antibody (A01546-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trp2-dct-picoband-trade-antibody-a01830-3-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01830-3-dct-primary-antibodies-wb-testing-1.jpgAnti-TRP2/DCT Antibody Picoband® Western blot analysis of TRP2/DCT using anti-TRP2/DCT antibody (A01830-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRP2/DCT antigen affinity purified polyclonal antibody (Catalog # A01830-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRP2/DCT at approximately 59 kDa. The expected band size for TRP2/DCT is at 59 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01830-3-dct-primary-antibodies-fcm-testing-2.pngAnti-TRP2/DCT Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TRP2/DCT antibody (A01830-3). Overlay histogram showing MCF-7 cells stained with A01830-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TRP2/DCT Antibody (A01830-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dnmt3b-picoband-trade-antibody-a00319-4-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00319-4-dnmt3b-primary-antibodies-wb-testing-1.jpgAnti-DNMT3B Antibody Picoband® Western blot analysis of DNMT3B using anti-DNMT3B antibody (A00319-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B antigen affinity purified polyclonal antibody (Catalog # A00319-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3B at approximately 96 kDa. The expected band size for DNMT3B is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00319-4-dnmt3b-primary-antibodies-fcm-testing-2.pngAnti-DNMT3B Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-DNMT3B antibody (A00319-4). Overlay histogram showing Caco-2 cells stained with A00319-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT3B Antibody (A00319-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dnmt3l-picoband-trade-antibody-a04214-3-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04214-3-dnmt3l-primary-antibodies-wb-testing-1.jpgAnti-DNMT3L Antibody Picoband® Western blot analysis of DNMT3L using anti-DNMT3L antibody (A04214-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3L antigen affinity purified polyclonal antibody (Catalog # A04214-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3L at approximately 44 kDa. The expected band size for DNMT3L is at 44 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04214-3-dnmt3l-primary-antibodies-fcm-testing-2.pngAnti-DNMT3L Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-DNMT3L antibody (A04214-3). Overlay histogram showing Caco-2 cells stained with A04214-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT3L Antibody (A04214-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-desmoglein-3-pva-dsg3-picoband-trade-antibody-a03093-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03093-dsg3-primary-antibodies-wb-testing-1.jpgAnti-Desmoglein 3/PVA/DSG3 Antibody Picoband® Western blot analysis of Desmoglein 3/PVA/DSG3 using anti-Desmoglein 3/PVA/DSG3 antibody (A03093). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoglein 3/PVA/DSG3 antigen affinity purified polyclonal antibody (Catalog # A03093) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoglein 3/PVA/DSG3 at approximately 130 kDa. The expected band size for Desmoglein 3/PVA/DSG3 is at 130 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03093-dsg3-primary-antibodies-fcm-testing-2.pngAnti-Desmoglein 3/PVA/DSG3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Desmoglein 3/PVA/DSG3 antibody (A03093). Overlay histogram showing HepG2 cells stained with A03093 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Desmoglein 3/PVA/DSG3 Antibody (A03093, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-desmoplakin-dsp-picoband-trade-antibody-a00616-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00616-2-dsp-primary-antibodies-wb-testing-1.jpgAnti-Desmoplakin/DSP Antibody Picoband® Western blot analysis of Desmoplakin/DSP using anti-Desmoplakin/DSP antibody (A00616-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmoplakin/DSP antigen affinity purified polyclonal antibody (Catalog # A00616-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmoplakin/DSP at approximately 250 kDa. The expected band size for Desmoplakin/DSP is at 250 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00616-2-dsp-primary-antibodies-fcm-testing-2.pngAnti-Desmoplakin/DSP Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-Desmoplakin/DSP antibody (A00616-2). Overlay histogram showing PC-3 cells stained with A00616-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Desmoplakin/DSP Antibody (A00616-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-egr1-picoband-trade-antibody-a00687-3-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00687-3-egr1-primary-antibodies-wb-testing-1.jpgAnti-EGR1 Antibody Picoband® Western blot analysis of EGR1 using anti-EGR1 antibody (A00687-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGR1 antigen affinity purified polyclonal antibody (Catalog # A00687-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGR1 at approximately 60 kDa. The expected band size for EGR1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00687-3-egr1-primary-antibodies-fcm-testing-2.pngAnti-EGR1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-EGR1 antibody (A00687-3). Overlay histogram showing K562 cells stained with A00687-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EGR1 Antibody (A00687-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00687-3-egr1-primary-antibodies-fcm-testing-3.pngAnti-EGR1 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-EGR1 antibody (A00687-3). Overlay histogram showing RAW264.7 cells stained with A00687-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EGR1 Antibody (A00687-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00687-3-egr1-primary-antibodies-fcm-testing-4.pngAnti-EGR1 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-EGR1 antibody (A00687-3). Overlay histogram showing RH35 cells stained with A00687-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EGR1 Antibody (A00687-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eno1-picoband-trade-antibody-a01250-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-wb-testing-1.jpgAnti-ENO1 Antibody Picoband® Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (Catalog # A01250-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO1 at approximately 50 kDa. The expected band size for ENO1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-2.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-3.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-4.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-5.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-6.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-7.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-8.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-ihc-testing-9.jpgAnti-ENO1 Antibody Picoband® IHC analysis of ENO1 using anti-ENO1 antibody (A01250-2). ENO1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1 Antibody (A01250-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01250-2-eno1-primary-antibodies-fcm-testing-10.pngAnti-ENO1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-ENO1 antibody (A01250-2). Overlay histogram showing K562 cells stained with A01250-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENO1 Antibody (A01250-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fgf4-picoband-trade-antibody-a01675-1-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01675-1-fgf4-primary-antibodies-ihc-testing-1.jpgAnti-FGF4 Antibody IHC analysis of FGF4 using anti-FGF4 antibody (A01675-1). FGF4 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF4 Antibody (A01675-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01675-1-fgf4-primary-antibodies-ihc-testing-2.jpgAnti-FGF4 Antibody IHC analysis of FGF4 using anti-FGF4 antibody (A01675-1). FGF4 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF4 Antibody (A01675-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01675-1-fgf4-primary-antibodies-ihc-testing-3.jpgAnti-FGF4 Antibody IHC analysis of FGF4 using anti-FGF4 antibody (A01675-1). FGF4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF4 Antibody (A01675-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-hemopexin-hpx-picoband-trade-antibody-a02237-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02237-2-hpx-primary-antibodies-wb-testing-1.jpgAnti-Hemopexin/HPX Antibody Picoband® Western blot analysis of Hemopexin/HPX using anti-Hemopexin/HPX antibody (A02237-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemopexin/HPX antigen affinity purified polyclonal antibody (Catalog # A02237-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemopexin/HPX at approximately 72 kDa. The expected band size for Hemopexin/HPX is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-icam1-picoband-trade-antibody-a00171-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00171-2-icam1-primary-antibodies-wb-testing-1.jpgAnti-ICAM1 Antibody Picoband® Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 95 kDa. The expected band size for ICAM1 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00171-2-icam1-primary-antibodies-fcm-testing-2.pngAnti-ICAM1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-ICAM1 antibody (A00171-2). Overlay histogram showing HepG2 cells stained with A00171-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICAM1 Antibody (A00171-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-integrin-alpha-3-itga3-picoband-trade-antibody-a02902-1-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-1-itga3-primary-antibodies-wb-testing-1_1.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® Western blot analysis of Integrin Alpha 3/ITGA3 using anti-Integrin Alpha 3/ITGA3 antibody (A02902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 3/ITGA3 antigen affinity purified polyclonal antibody (Catalog # A02902-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 3/ITGA3 at approximately 130 kDa. The expected band size for Integrin Alpha 3/ITGA3 is at 130 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-1-itga3-primary-antibodies-ihc-testing-2.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IHC analysis of Integrin alpha 3/ITGA3 using anti-Integrin alpha 3/ITGA3 antibody (A02902-1). Integrin alpha 3/ITGA3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Integrin alpha 3/ITGA3 Antibody (A02902-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-1-itga3-primary-antibodies-ihc-testing-3.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IHC analysis of Integrin alpha 3/ITGA3 using anti-Integrin alpha 3/ITGA3 antibody (A02902-1). Integrin alpha 3/ITGA3 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Integrin alpha 3/ITGA3 Antibody (A02902-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-1-itga3-primary-antibodies-ihc-testing-4.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IHC analysis of Integrin alpha 3/ITGA3 using anti-Integrin alpha 3/ITGA3 antibody (A02902-1). Integrin alpha 3/ITGA3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Integrin alpha 3/ITGA3 Antibody (A02902-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-1-itga3-primary-antibodies-if-testing-5.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® IF analysis of Integrin alpha 3/ITGA3 using anti-Integrin alpha 3/ITGA3 antibody (A02902-1). Integrin alpha 3/ITGA3 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Integrin alpha 3/ITGA3 Antibody (A02902-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02902-1-itga3-primary-antibodies-fcm-testing-6.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Integrin alpha 3/ITGA3 antibody (A02902-1). Overlay histogram showing U87 cells stained with A02902-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 3/ITGA3 Antibody (A02902-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-c-jun-jun-picoband-trade-antibody-a02038-3-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02038-3-jun-primary-antibodies-wb-testing-1.jpgAnti-c-Jun/JUN Antibody Picoband® Western blot analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Jun/JUN antigen affinity purified polyclonal antibody (Catalog # A02038-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Jun/JUN at approximately 36 kDa. The expected band size for C-Jun/JUN is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02038-3-12967_2012_article_1482_fig6_html.jpgAnti-c-Jun/JUN Antibody Picoband®Hirsutanol A activated JNK signaling pathway through elevating of ROS production and blockage of JNK signal pathway increased apoptosis and elevated ROS level induced by hirsutanol A. SW620 cells were treated with 20 μmol/L hirsutanol A for indicated times or various concentrations of hirsutanol A for 24 h. The expression of p-JNK and p-c-Jun were detected by immunoblotting assay ( A ). SW620 cells were pre-incubated with NAC for 1 h, then treated with 20 μmol/L hirsutanol A for 24 h. The expression of p-JNK was determined by immunoblotting assay ( B ). SW620 cells were pre-incubated with SP600125 for 1 h , then treated with 20 μmol/L hirsutanol A for 72 h. Cell growth inhibition were detected by MTT assay ( C ). SW620 cells were pre-incubated with SP600125 for 1 h , then treated with 20 μmol/L hirsutanol A for 48 h. Cell apoptosis was detected by AnnexinV/PI analysis ( D ). SW620 cells were pre-incubated with SP600125 for 1 h or transfected with JNK- siRNA to block JNK signaling pathway, then treated with 20 μmol/L hirsutanol A for 3 h. Cellular ROS level was monitored by flow cytometry. Results are presented as means ± SD from 3 independent experiments. (** < 0.05 versus *; *** < 0.01 versus *) ( E ). Index in PubMed under a CC BY license. PMID: <a href='https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-32'>23394457</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02038-3-jun-primary-antibodies-ihc-testing-2.jpgAnti-c-Jun/JUN Antibody Picoband® IHC analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3). C-Jun/JUN was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C-Jun/JUN Antibody (A02038-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02038-3-jun-primary-antibodies-ihc-testing-3.jpgAnti-c-Jun/JUN Antibody Picoband® IHC analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3). C-Jun/JUN was detected in a paraffin-embedded section of human the renal pelvis squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C-Jun/JUN Antibody (A02038-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02038-3-jun-primary-antibodies-if-testing-4.jpgAnti-c-Jun/JUN Antibody Picoband® IF analysis of C-Jun/JUN using anti-C-Jun/JUN antibody (A02038-3) and anti-Beta Tubulin antibody (M01857-3). C-Jun/JUN was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C-Jun/JUN beta Antibody (A02038-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02038-3-jun-primary-antibodies-fcm-testing-5_1.pngAnti-c-Jun/JUN Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-C-Jun/JUN antibody (A02038-3). Overlay histogram showing U20S cells stained with A02038-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Jun/JUN Antibody (A02038-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-keratocan-ktn-kera-picoband-trade-antibody-a07712-1-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07712-1-kera-primary-antibodies-wb-testing-1.jpgAnti-Keratocan/KTN/Kera Antibody Picoband® Western blot analysis of Keratocan/KTN/Kera using anti-Keratocan/KTN/Kera antibody (A07712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keratocan/KTN/Kera antigen affinity purified polyclonal antibody (Catalog # A07712-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keratocan/KTN/Kera at approximately 41 kDa. The expected band size for Keratocan/KTN/Kera is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-mt-co2-picoband-trade-antibody-a03631-1-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03631-1-mt-co2-primary-antibodies-wb-testing-1.jpgAnti-MT-CO2 Antibody Picoband® Western blot analysis of MT-CO2 using anti-MT-CO2 antibody (A03631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-CO2 antigen affinity purified polyclonal antibody (Catalog # A03631-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-CO2 at approximately 21 kDa. The expected band size for MT-CO2 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03631-1-mt-co2-primary-antibodies-wb-testing-2.jpgAnti-MT-CO2 Antibody Picoband® Western blot analysis of MT-CO2 using anti-MT-CO2 antibody (A03631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat small intestine tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-CO2 antigen affinity purified polyclonal antibody (Catalog # A03631-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MT-CO2 at approximately 21 kDa. The expected band size for MT-CO2 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03631-1-mt-co2-primary-antibodies-ihc-testing-3.jpgAnti-MT-CO2 Antibody Picoband® IHC analysis of MT-CO2 using anti-MT-CO2 antibody (A03631-1). MT-CO2 was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MT-CO2 Antibody (A03631-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03631-1-mt-co2-primary-antibodies-ihc-testing-4.jpgAnti-MT-CO2 Antibody Picoband® IHC analysis of MT-CO2 using anti-MT-CO2 antibody (A03631-1). MT-CO2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MT-CO2 Antibody (A03631-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03631-1-mt-co2-primary-antibodies-ihc-testing-5.jpgAnti-MT-CO2 Antibody Picoband® IHC analysis of MT-CO2 using anti-MT-CO2 antibody (A03631-1). MT-CO2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MT-CO2 Antibody (A03631-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03631-1-mt-co2-primary-antibodies-ihc-testing-6.jpgAnti-MT-CO2 Antibody Picoband® IHC analysis of MT-CO2 using anti-MT-CO2 antibody (A03631-1). MT-CO2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MT-CO2 Antibody (A03631-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03631-1-mt-co2-primary-antibodies-fcm-testing-7.pngAnti-MT-CO2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-MT-CO2 antibody (A03631-1). Overlay histogram showing U87 cells stained with A03631-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT-CO2 Antibody (A03631-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nd6-mt-nd6-picoband-trade-antibody-a03777-2-boster.html2026-03-17T05:13:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03777-2-mt-nd2-primary-antibodies-wb-testing-1.jpgAnti-ND6/MT-ND6 Antibody Picoband® Western blot analysis of ND6/MT-ND6 using anti-ND6/MT-ND6 antibody (A03777-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ND6/MT-ND6 antigen affinity purified polyclonal antibody (Catalog # A03777-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ND6/MT-ND6 at approximately 19 kDa. The expected band size for ND6/MT-ND6 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03777-2-mt-nd2-primary-antibodies-if-testing-2.jpgAnti-ND6/MT-ND6 Antibody Picoband® IF analysis of ND6/MT-ND6 using anti-ND6/MT-ND6 antibody (A03777-2). ND6/MT-ND6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ND6/MT-ND6 Antibody (A03777-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03777-2-mt-nd2-primary-antibodies-fcm-testing-3.pngAnti-ND6/MT-ND6 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-ND6/MT-ND6 antibody (A03777-2). Overlay histogram showing HepG2 cells stained with A03777-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ND6/MT-ND6 Antibody (A03777-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-myd88-picoband-trade-antibody-a00025-3-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00025-3-myd88-primary-antibodies-wb-testing-1.jpgAnti-MYD88 Antibody Picoband® Western blot analysis of MYD88 using anti-MYD88 antibody (A00025-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # A00025-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 37 kDa. The expected band size for MYD88 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00025-3-jvs-21-e50-g006.jpgAnti-MYD88 Antibody Picoband®The detection of NF-κB signaling pathway-related genes during pEGFP-N1-NS1 transfection. The expression of MyD88 (A), IRAK4 (D), TRAF6 (E), and TAK1 (F) mRNA in the 293T cells were tested after pEGFP-N1-NS1 transfection at different time points. The mock- and 4 μg pEGFP-N1-transfected 293T cells were used as the controls. The level of MyD88 protein expression was confirmed after transfection with 4 μg pEGFP-N1-NS1 by western blot (B) and the empty vector control test at the protein level were used to detect the expression of MyD88 (C). Compared with the control group at the same time, there was a highly significant difference between groups ( ** p < 0.01), or significant difference between groups ( * p < 0.05, Student's t -test). NF-κB, nuclear factor kappa B. Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263913/'>32476323</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00025-3-myd88-primary-antibodies-ihc-testing-2.jpgAnti-MYD88 Antibody Picoband® IHC analysis of MYD88 using anti-MYD88 antibody (A00025-3). MYD88 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYD88 Antibody (A00025-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00025-3-myd88-primary-antibodies-fcm-testing-3.pngAnti-MYD88 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-MYD88 antibody (A00025-3). Overlay histogram showing Caco-2 cells stained with A00025-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYD88 Antibody (A00025-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufb8-picoband-trade-antibody-a07936-1-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-wb-testing-1.jpgAnti-NDUFB8 Antibody Picoband® Western blot analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB8 antigen affinity purified polyclonal antibody (Catalog # A07936-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB8 at approximately 19-22 kDa. The expected band size for NDUFB8 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-ihc-testing-2.jpgAnti-NDUFB8 Antibody Picoband® IHC analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). NDUFB8 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFB8 Antibody (A07936-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-ihc-testing-3.jpgAnti-NDUFB8 Antibody Picoband® IHC analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). NDUFB8 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFB8 Antibody (A07936-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-ihc-testing-4.jpgAnti-NDUFB8 Antibody Picoband® IHC analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). NDUFB8 was detected in a paraffin-embedded section of human SM fatty carcinoma of the left kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFB8 Antibody (A07936-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-ihc-testing-5.jpgAnti-NDUFB8 Antibody Picoband® IHC analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). NDUFB8 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFB8 Antibody (A07936-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-ihc-testing-6.jpgAnti-NDUFB8 Antibody Picoband® IHC analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). NDUFB8 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFB8 Antibody (A07936-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-if-testing-7.jpgAnti-NDUFB8 Antibody Picoband® IF analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). NDUFB8 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDUFB8 Antibody (A07936-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07936-1-ndufbb-primary-antibodies-fcm-testing-8.pngAnti-NDUFB8 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-NDUFB8 antibody (A07936-1). Overlay histogram showing HEL cells stained with A07936-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB8 Antibody (A07936-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-upa-receptor-u-par-plaur-picoband-trade-antibody-a00993-4-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00993-4-plaur-primary-antibodies-wb-testing-1.jpgAnti-uPA Receptor/U-PAR/PLAUR Antibody Picoband® Western blot analysis of UPA Receptor/U-PAR/PLAUR using anti-UPA Receptor/U-PAR/PLAUR antibody (A00993-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPA Receptor/U-PAR/PLAUR antigen affinity purified polyclonal antibody (Catalog # A00993-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPA Receptor/U-PAR/PLAUR at approximately 45 kDa. The expected band size for UPA Receptor/U-PAR/PLAUR is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ppar-gamma-pparg-picoband-trade-antibody-a00449-3-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00449-3-pparg-primary-antibodies-wb-testing-1.jpgAnti-PPAR gamma/PPARG Antibody Picoband® Western blot analysis of PPAR Gamma/PPARG using anti-PPAR Gamma/PPARG antibody (A00449-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR Gamma/PPARG antigen affinity purified polyclonal antibody (Catalog # A00449-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAR Gamma/PPARG at approximately 65 kDa. The expected band size for PPAR Gamma/PPARG is at 65 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00449-3-fcell-12-1503481-g004.jpgAnti-PPAR gamma/PPARG Antibody Picoband®ANXA1 mediated PPARγ-CEBPα pathway to regulate osteoclast differentiation (A) The mRNA level of PPARγ in RAW264.7 cultured with siANXA1-EVs. (B) The mRNA level of CEBPα in RAW264.7 cultured with siANXA1-EVs. (C) The protein level of PPARγ and CEBPα in RAW264.7 cultured with siANXA1-EVs. (D) Quantitative analysis of PPARγ protein expression. (E) Quantitative analysis of CEBPα protein expression. (F) Schematic illustration of PPARγ inhibited RAW264.7 and DFSC-EVs co-culture system. (G) Representative images of TRAP staining. Scale bar = 200 μm. (H) Quantitative analysis of TRAP-positive area. (I) PPARγ inhibited RAW264.7 construction. (J) The mRNA level of CEBPα in PPARγ inhibited RAW264.7. (K) The protein level of PPARγ and CEBPα in PPARγ inhibited RAW264.7. (L) Quantitative analysis of PPARγ protein expression. (M) Quantitative analysis of CEBPα protein expression. (N) The mRNA level of ACP5 , CTSK and CFOS in PPARγ inhibited RAW264.7. (O) The protein level of ACP5, CTSK and CFOS in PPARγ inhibited RAW264.7. (P) Western blotting quantification. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00449-3-fcell-12-1503481-g005.jpgAnti-PPAR gamma/PPARG Antibody Picoband®DFSCs-EVs/ANXA1 regulating tooth eruption by affecting osteoclast differentiation. (A) Representative micro-CT images of detecting tooth eruption distance. (B) Analysis of tooth eruption distance based on micro-CT. (C) Representative H&E staining images of the first mandibular molar area. (D) Analysis of tooth eruption distance based on H&E staining. (E) Representative images of TRAP staining. (F) Quantitative analysis of TRAP-positive area. (G) Representative immunohistochemistry staining images of PPARγ expression in the first mandibular molar area. (H) Quantitative analysis of PPARγ expression in the first mandibular molar area. (I) Representative immunohistochemistry staining images of CEBPα expression in the first mandibular molar area. (J) Quantitative analysis of CEBPα expression in the first mandibular molar area. ns, not significant. Scale bar = 1 mm ** p < 0. 01. n = 3. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00449-3-pparg-primary-antibodies-if-testing-2.jpgAnti-PPAR gamma/PPARG Antibody Picoband® IF analysis of PPAR gamma/PPARG using anti-PPAR gamma/PPARG antibody (A00449-3). PPAR gamma/PPARGNOX4 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPAR gamma/PPARG Antibody (A00449-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00449-3-pparg-primary-antibodies-fcm-testing-3.pngAnti-PPAR gamma/PPARG Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PPAR Gamma/PPARG antibody (A00449-3). Overlay histogram showing A431 cells stained with A00449-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAR Gamma/PPARG Antibody (A00449-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-proteasome-20s-beta-7-psmb7-picoband-trade-antibody-a08095-1-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-wb-testing-1.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® Western blot analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6; human HepG2 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Proteasome 20S Beta 7/PSMB7 antigen affinity purified polyclonal antibody (Catalog # A08095-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Proteasome 20S Beta 7/PSMB7 at approximately 26 kDa. The expected band size for Proteasome 20S Beta 7/PSMB7 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-wb-testing-2.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® Western blot analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6; mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Proteasome 20S Beta 7/PSMB7 antigen affinity purified polyclonal antibody (Catalog # A08095-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Proteasome 20S Beta 7/PSMB7 at approximately 26 kDa. The expected band size for Proteasome 20S Beta 7/PSMB7 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-3.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-4.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-5.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-6.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of human cervical intraepithelial neoplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-7.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-8.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of human invasive breast carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-9.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-ihc-testing-10.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IHC analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-if-testing-11.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® IF analysis of Proteasome 20S Beta 7/PSMB7 using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Proteasome 20S Beta 7/PSMB7 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08095-1-psmb7-primary-antibodies-fcm-testing-12.pngAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Proteasome 20S Beta 7/PSMB7 antibody (A08095-1). Overlay histogram showing U87 cells stained with A08095-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Proteasome 20S Beta 7/PSMB7 Antibody (A08095-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rab5a-b-c-picoband-trade-antibody-a01891-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01891-rab5-primary-antibodies-wb-testing-1.jpgAnti-RAB5A/B/C Antibody Picoband® Western blot analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5A/B/C antigen affinity purified polyclonal antibody (Catalog # A01891) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5A/B/C at approximately 24 kDa. The expected band size for RAB5A/B/C is at 24 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01891-rab5-primary-antibodies-ihc-testing-2.jpgAnti-RAB5A/B/C Antibody Picoband® IHC analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). RAB5A/B/C was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A/B/C Antibody (A01891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01891-rab5-primary-antibodies-ihc-testing-3.jpgAnti-RAB5A/B/C Antibody Picoband® IHC analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). RAB5A/B/C was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A/B/C Antibody (A01891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01891-rab5-primary-antibodies-ihc-testing-4.jpgAnti-RAB5A/B/C Antibody Picoband® IHC analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). RAB5A/B/C was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A/B/C Antibody (A01891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01891-rab5-primary-antibodies-ihc-testing-5.jpgAnti-RAB5A/B/C Antibody Picoband® IHC analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). RAB5A/B/C was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A/B/C Antibody (A01891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01891-rab5-primary-antibodies-ihc-testing-6.jpgAnti-RAB5A/B/C Antibody Picoband® IHC analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). RAB5A/B/C was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A/B/C Antibody (A01891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01891-rab5-primary-antibodies-if-testing-7.jpgAnti-RAB5A/B/C Antibody Picoband® IF analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). RAB5A/B/C was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB5A/B/C Antibody (A01891) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rangap1-picoband-trade-antibody-a02771-2-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02771-2-rangap1-primary-antibodies-wb-testing-1.jpgAnti-RANGAP1 Antibody Picoband® Western blot analysis of RANGAP1 using anti-RANGAP1 antibody (A02771-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANGAP1 antigen affinity purified polyclonal antibody (Catalog # A02771-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANGAP1 at approximately 70 kDa. The expected band size for RANGAP1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02771-2-rangap1-primary-antibodies-wb-testing-2.jpgAnti-RANGAP1 Antibody Picoband® Western blot analysis of RANGAP1 using anti-RANGAP1 antibody (A02771-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: rat small intestine tissue lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse brain tissue lysates, Lane 2: mouse stomach tissue lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANGAP1 antigen affinity purified polyclonal antibody (Catalog # A02771-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANGAP1 at approximately 70 kDa. The expected band size for RANGAP1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02771-2-rangap1-primary-antibodies-ihc-testing-3.jpgAnti-RANGAP1 Antibody Picoband® IHC analysis of RANGAP1 using anti-RANGAP1 antibody (A02771-2). RANGAP1 was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RANGAP1 Antibody (A02771-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02771-2-rangap1-primary-antibodies-if-testing-4.jpgAnti-RANGAP1 Antibody Picoband® IF analysis of RANGAP1 using anti-RANGAP1 antibody (A02771-2). RANGAP1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RANGAP1 Antibody (A02771-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02771-2-rangap1-primary-antibodies-fcm-testing-5.pngAnti-RANGAP1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-RANGAP1 antibody (A02771-2). Overlay histogram showing U87 cells stained with A02771-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RANGAP1 Antibody (A02771-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sp6-picoband-trade-antibody-a08730-3-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08730-3-sp6-primary-antibodies-wb-testing-1.jpgAnti-SP6 Antibody Picoband® Western blot analysis of SP6 using anti-SP6 antibody (A08730-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP6 antigen affinity purified polyclonal antibody (Catalog # A08730-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP6 at approximately 43 kDa. The expected band size for SP6 is at 43 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08730-3-sp6-primary-antibodies-fcm-testing-2.pngAnti-SP6 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SP6 antibody (A08730-3). Overlay histogram showing K562 cells stained with A08730-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SP6 Antibody (A08730-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nak-tbk1-picoband-trade-antibody-a00261-1-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00261-1-tbk1-primary-antibodies-wb-testing-1.jpgAnti-NAK/TBK1 Antibody Picoband® Western blot analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (A00261-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human LNCAP whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAK/TBK1 antigen affinity purified polyclonal antibody (Catalog # A00261-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAK/TBK1 at approximately 84 kDa. The expected band size for NAK/TBK1 is at 84 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00261-1-12276_2025_1457_fig7_html.pngAnti-NAK/TBK1 Antibody Picoband®HMGCR inhibition combined with radiotherapy significantly activates the cGAS–STING pathway. a , A volcano plot of differentially expressed genes between the combination group and the radiotherapy group. b GSEA of the TCR signaling pathway (KEGG: MMU04660) and T-cell-mediated immunity (GO: 0002456) between the combination therapy group and the radiotherapy group. c A heatmap of log 2 FC to depict the gene expression associated with type I IFN. d The expression of p-TBK1, p-IRF3, TBK1 and IRF3 protein extracted from CT26 tumors was detected by western blot. e , The relative expression of CCL5, CXCL10 and IFNβ mRNA extracted from CT26 tumors was detected by qPCR. f , Representative IF images of p-TBK1 and p-IRF3. g , Representative IHC images of IFN-β. h , The levels of IFN-β, CCL5 and IFN-γ protein in tumor tissues were measured via ELISA. Scale bar, 50 μm. ** P < 0.01; * P < 0.05. Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s12276-025-01457-6'>40355720</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00261-1-12276_2025_1457_fig4_html.pngAnti-NAK/TBK1 Antibody Picoband®Cholesterol impairs radiotherapy-induced cGAS–STING activation and lovastatin rescues this activation in vitro. a The expression of p-TBK1, p-IRF3, TBK1 and IRF3 protein extracted from CT26 cells with different treatments (ctrl, cholesterol 50 μM, MβCD 2 mM, IR 6 Gy, IR + cholesterol and IR + MβCD) and detected by western blot. b The relative expression of CCL5, CXCL10 and IFNβ mRNA extracted from CT26 cells was detected by qPCR. c The expression of p-TBK1, p-IRF3, TBK1 and IRF3 protein was extracted from HCT116 cells with different treatments (ctrl, cholesterol 50 μM, MβCD 2 mM, IR 6 Gy, IR + cholesterol and IR + MβCD) and detected by western blot. d The relative expression of CCL5, CXCL10 and IFNβ mRNA extracted from HCT116 cells was detected by qPCR. e , f The expression of p-TBK1, p-IRF3, TBK1 and IRF3 protein extracted from CT26 cells and HCT116 cells with different treatments (ctrl, lovastatin 10 μM, IR 6 Gy and IR + lovastatin) was detected by western blot ( e ) and quantitative anslysis ( f ). g , h , Confocal fluorescence microscopy was conducted on CT26 ( g ) and HCT116 ( h ) cells with different treatments. The cells were labeled with DAPI (blue) and p-TBK1 (green) or p-IRF3 (green). i The levels of IFN-β and IFN-γ in the supernatant of co-cultures of MC38-OVA cells and OT-1 mouse spleen cells as well as HCT116 cells and human PBMCs were measured using ELISA. The ratio of immune cells to tumor cells is 10:1. The co-cultures were maintained for 36 h. j LDH release assay was performed using the supernatants from co-cultures of MC38-OVA cells and OT-1 mouse spleen cells as well as HCT116 cells and human PBMCs. The ratio of immune cells to tumor cells is 10:1. The co-cultures were maintained for 36 h. Scale bar, 5 μm. ** P < 0.01; * P < 0.05; ns, not significant. Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s12276-025-01457-6'>40355720</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00261-1-tbk1-primary-antibodies-ihc-testing-2.jpgAnti-NAK/TBK1 Antibody Picoband® IHC analysis of NAK/TBK1 using anti-NAK/TBK1 antibody (A00261-1). NAK/TBK1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAK/TBK1 Antibody (A00261-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-53bp1-tp53bp1-picoband-trade-antibody-a00397-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-wb-testing-1.jpgAnti-53BP1/TP53BP1 Antibody Picoband® Western blot analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-53BP1/TP53BP1 antigen affinity purified polyclonal antibody (Catalog # A00397) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 53BP1/TP53BP1 at approximately 450 kDa. The expected band size for 53BP1/TP53BP1 is at 214 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-wb-testing-2.jpgAnti-53BP1/TP53BP1 Antibody Picoband®Western blot analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS (Cell nucleus) whole cell lysates, Lane 2: human U2OS (Cell nucleus) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-53BP1/TP53BP1 antigen affinity purified polyclonal antibody (Catalog # A00397) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for 53BP1/TP53BP1 at approximately 450 kDa. The expected band size for 53BP1/TP53BP1 is at 214 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-2.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-3.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-4.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-5.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-6.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-7.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-8.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-ihc-testing-9.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IHC analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-if-testing-10.jpgAnti-53BP1/TP53BP1 Antibody Picoband® IF analysis of 53BP1/TP53BP1 using anti-53BP1/TP53BP1 antibody (A00397). 53BP1/TP53BP1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-53BP1/TP53BP1 Antibody (A00397) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00397-tp53bp1-primary-antibodies-fcm-testing-11.pngAnti-53BP1/TP53BP1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-53BP1/TP53BP1 antibody (A00397). Overlay histogram showing Hela cells stained with A00397 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-53BP1/TP53BP1 Antibody (A00397, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tpr-picoband-trade-antibody-a00695-2-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-2-tpr-primary-antibodies-wb-testing-1.jpgAnti-TPR Antibody Picoband® Western blot analysis of TPR using anti-TPR antibody (A00695-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPR antigen affinity purified polyclonal antibody (Catalog # A00695-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPR at approximately 267 kDa. The expected band size for TPR is at 267 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-2-tpr-primary-antibodies-ihc-testing-2.jpgAnti-TPR Antibody Picoband® IHC analysis of TPR using anti-TPR antibody (A00695-2). TPR was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPR Antibody (A00695-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-2-tpr-primary-antibodies-ihc-testing-3.jpgAnti-TPR Antibody Picoband® IHC analysis of TPR using anti-TPR antibody (A00695-2). TPR was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPR Antibody (A00695-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-2-tpr-primary-antibodies-ihc-testing-4.jpgAnti-TPR Antibody Picoband® IHC analysis of TPR using anti-TPR antibody (A00695-2). TPR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPR Antibody (A00695-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-2-tpr-primary-antibodies-if-testing-5.jpgAnti-TPR Antibody Picoband® IF analysis of TPR using anti-TPR antibody (A00695-2). TPR was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TPR Antibody (A00695-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-2-tpr-primary-antibodies-if-testing-6.jpgAnti-TPR Antibody Picoband® IF analysis of TPR using anti-TPR antibody (A00695-2). TPR was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TPR Antibody (A00695-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00695-2-tpr-primary-antibodies-fcm-testing-7.pngAnti-TPR Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-TPR antibody (A00695-2). Overlay histogram showing Jurkat cells stained with A00695-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPR Antibody (A00695-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trpm2-picoband-trade-antibody-a01013-1-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01013-1-trpm2-primary-antibodies-wb-testing-1.jpgAnti-TRPM2 Antibody Picoband® Western blot analysis of TRPM2 using anti-TRPM2 antibody (A01013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM2 antigen affinity purified polyclonal antibody (Catalog # A01013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM2 at approximately 171 kDa. The expected band size for TRPM2 is at 171 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01013-1-trpm2-primary-antibodies-wb-testing-2.jpgAnti-TRPM2 Antibody Picoband®Western blot analysis of TRPM2 using anti-TRPM2 antibody (A01013-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions. Lane 1: mouse RAW 264.7 whole cell lysates, Lane 2: mouse J774A.1 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM2 antigen affinity purified polyclonal antibody (A01013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRPM2 at approximately 180 kDa. The expected band size for TRPM2 is at 170 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01013-1-trpm2-primary-antibodies-fcm-testing-2.pngAnti-TRPM2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TRPM2 antibody (A01013-1). Overlay histogram showing THP-1 cells stained with A01013-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPM2 Antibody (A01013-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trpv1-picoband-trade-antibody-a00128-4-boster.html2026-03-17T05:16:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00128-4-fphar-16-1541684-g004.jpgAnti-TRPV1 Antibody Picoband®Expression of TRPV1 and SP. (A) The expression of TRPV1 in the L6-S1 DRG and the expression of SP in the L6-S1 spinal cord. Statistical results analysis of TRPV1 in the L6-S1 DRG (B) and SP in the L6-S1 spinal cord (C) between groups. Differences between groups were compared by a one-factor ANOVA. Data represents the mean ± SD, *Significant difference compared with the Control group or prostatitis group, *p < 0.05; ***p < 0.001; ****p < 0.0001; “ns” indicates p > 0.05. Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11893827/'>40070569</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00128-4-trpv1-primary-antibodies-wb-testing-1.jpgAnti-TRPV1 Antibody Picoband® Western blot analysis of TRPV1 using anti-TRPV1 antibody (A00128-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV1 antigen affinity purified polyclonal antibody (Catalog # A00128-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV1 at approximately 95 kDa. The expected band size for TRPV1 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00128-4-trpv1-primary-antibodies-if-testing-2.jpgAnti-TRPV1 Antibody Picoband® IF analysis of TRPV1 using anti-TRPV1 antibody (A00128-4). TRPV1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRPV1 Antibody (A00128-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00128-4-trpv1-primary-antibodies-wb-testing-2.pngAnti-TRPV1 Antibody Picoband® Western blot analysis of TRPV1 using anti-TRPV1 antibody (A00128-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Control-rat MADB106 breast cancer cells, Lane 2: Agonist treatment group-rat MADB106 breast cancer cells, Lane 3: Inhibitor treatment group-rat MADB106 breast cancer cells. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV1 antigen affinity purified polyclonal antibody (Catalog # A00128-4) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. The expected band size for TRPV1 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00128-4-trpv1-primary-antibodies-fcm-testing-3.pngAnti-TRPV1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TRPV1 antibody (A00128-4). Overlay histogram showing U87 cells stained with A00128-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV1 Antibody (A00128-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ucp3-picoband-trade-antibody-a01769-3-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01769-3-ucp3-primary-antibodies-wb-testing-1.jpgAnti-UCP3 Antibody Picoband® Western blot analysis of UCP3 using anti-UCP3 antibody (A01769-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCP3 antigen affinity purified polyclonal antibody (Catalog # A01769-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCP3 at approximately 30 kDa. The expected band size for UCP3 is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-zic1-picoband-trade-antibody-a05537-4-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05537-4-zic1-primary-antibodies-wb-testing-1.jpgAnti-ZIC1 Antibody Picoband® Western blot analysis of ZIC1 using anti-ZIC1 antibody (A05537-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC1 antigen affinity purified polyclonal antibody (Catalog # A05537-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIC1 at approximately 48 kDa. The expected band size for ZIC1 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pd-1-pdcd1-antibody-a00178-2-boster.html2026-03-10T04:33:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-ko-testing-1.jpgAnti-PD-1 PDCD1 AntibodyKO Validation in HeLa Cells Loading: 10 μg of HeLa WT cell lysates or PD-1 KO cell lysates. Antibodies: PD-1, A00178-2 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-kd-testing-2.jpgAnti-PD-1 PDCD1 AntibodyKD Validation in HeLa Cells Loading: 10 μg of HeLa WT cell lysates or PD-1 KD cell lysates. Antibodies: PD-1, A00178-2 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-wb-testing-3.jpgAnti-PD-1 PDCD1 AntibodyWestern Blot Validation in Human and Mouse Cell Lines Loading: 15 μg of lysates per lane. Antibodies: PD-1 A00178-2 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-wb-testing-4.jpgAnti-PD-1 PDCD1 AntibodyWestern Blot Validation in THP-1 Cell Lysate in the (A) absence and (B) presence of blocking peptide Loading: 15 μg of lysates per lane. Antibodies: PD-1, A00178-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-wb-testing-5.jpgAnti-PD-1 PDCD1 AntibodyWestern Blot Validation in Rat Thymus Cell Lysate Loading: 15 μg of lysates per lane. Antibodies: PD-1 A00178-2 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-ihc-testing-6.jpgAnti-PD-1 PDCD1 AntibodyImmunohistochemistry Validation of PD-1 in Human Tonsil Tissue Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (A00178-2) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-if-testing-7.jpgAnti-PD-1 PDCD1 AntibodyImmunohistochemistry Validation of PD-1 in Human Tonsil Tissue Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-1 antibody (A00178-2) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-2-pdcd1-primary-antibodies-ihc-testing-8.jpgAnti-PD-1 PDCD1 AntibodyImmunohistochemistry Validation of PD-1 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PD-1 antibody (A00178-2) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. https://www.bosterbio.com/picokine-elisa-kits/mouse-il-18-picokine-elisa-kit-ek0433-boster.html2026-03-10T04:33:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0433.jpgMouse IL-18 ELISA Kit PicoKine®Mouse IL-18 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erp57-picoband-trade-antibody-m01464-4-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01464-4-pdia3-primary-antibodies-wb-testing-1.jpgAnti-ERp57 Antibody Picoband® (monoclonal, 7E5) Western blot analysis of ERp57 using anti-ERp57 antibody (M01464-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Raji whoe cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ERp57 antigen affinity purified monoclonal antibody (Catalog # M01464-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ERp57 at approximately 57 kDa. The expected band size for ERp57 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01464-4-pdia3-primary-antibodies-ihc-testing-2.jpgAnti-ERp57 Antibody Picoband® (monoclonal, 7E5) IHC analysis of ERp57 using anti-ERp57 antibody (M01464-4). ERp57 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ERp57 Antibody (M01464-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01464-4-pdia3-primary-antibodies-fcm-testing-3.pngAnti-ERp57 Antibody Picoband® (monoclonal, 7E5) Flow Cytometry analysis of U87 cells using anti-ERp57 antibody (M01464-4). Overlay histogram showing U87 cells stained with M01464-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ERp57 Antibody (M01464-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-beclin-1-picoband-trade-antibody-m00327-2-boster.html2026-03-17T05:13:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-becn1-primary-antibodies-wb-testing-1.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3) Western blot analysis of Beclin 1 using anti-Beclin 1 antibody (M00327-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Beclin 1 antigen affinity purified monoclonal antibody (Catalog # M00327-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Beclin 1 at approximately 52-60 kDa. The expected band size for Beclin 1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-ajcr0006-0924-f4.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3)N+4: NDV+4-PBA; R+4: rL-RVG+4-PBA. The contribution of ER stress downregulation to the expression of autophagy- and apoptosis-associated proteins in SGC and HGC cells after infection. A. For SGC; B. For HGC. The expression of the autophagy-associated proteins beclin-1 and LC3-II/I decreased in cells with 4-PBA treatment compared with cells without 4-PBA treatment. The same was observed for apoptosis-associated proteins. C and D. Early apoptotic (annexin V+/PI-) and late apoptotic (annexin V+/PI+) cells. Prior to fluorescence-activated cell sorting analysis of annexin V/PI staining, SGC cells were pretreated with 4-PBA (10 mM) 2 h before exposure to NDV or rL-RVG (10 MOI). Annexin V/PI staining was then performed to assess apoptosis/necrosis. Annexin V staining showed that pretreatment with 10 mM 4-PBA resulted in a significant decrease in apoptosis in the rL-RVG-infected SGC cells (**, p<0.01). Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4889710/&orig_host=www.ncbi.nlm.nih.gov'>27293989</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-ajcr0006-0924-f5.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3)si+N: siCHOP+NDV; si+4: siCHOP+rL-RVG. Knockdown of CHOP decreased autophagy- and apoptosis-related protein expression. A. Fluorescently labeled siRNA (FAM-siRNA) was transferred into cells according to the manufacturer’s instructions, and the effects were monitored by immunofluorescence microscopy (x200 magnification). B. I expression of CHOP protein was silenced significantly in the group expressing siRNA 3. C. For SGC; D. For HGC. The expression of the autophagy-associated proteins beclin-1 and LC3-II/I decreased in cells after silencing CHOP compared with expression in cells without treatment. The same was observed for apoptosis-associated proteins. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4889710/&orig_host=www.ncbi.nlm.nih.gov'>27293989</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-ajcr0006-0924-f6.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3)SP+N: SP600125+NDV; SP+R: SP600125+rL-RVG. The autophagy induced by rL-RVG was mediated by the IRE1/JNK/beclin-1 signaling pathway. A. For SGC; B. For HGC. The phosphorylation of JNK and bcl-2 was upregulated in the rL-RVG- or NDV-infected SGC and HGC cells. The expression of the autophagy-associated proteins beclin-1 and LC3-II/I decreased in cells after treatment with a specific inhibitor of JNK, or SP600125, compared with cells without the treatment. The same was observed for apoptosis-associated proteins. C. The ultrastructure of the SGC cells after infection with virus or pretreatment with SP600125. rL-RVG infection increased the number of autophagosomes in SGC cells, but the number of rL-RVG-induced autophagosomes was markedly decreased after pretreatment with SP600125. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4889710/&orig_host=www.ncbi.nlm.nih.gov'>27293989</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-becn1-primary-antibodies-ihc-testing-2.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3) IHC analysis of Beclin 1 using anti-Beclin 1 antibody (M00327-2). Beclin 1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Beclin 1 Antibody (M00327-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-becn1-primary-antibodies-ihc-testing-3.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3) IHC analysis of Beclin 1 using anti-Beclin 1 antibody (M00327-2). Beclin 1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Beclin 1 Antibody (M00327-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-becn1-primary-antibodies-ihc-testing-4.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3) IHC analysis of Beclin 1 using anti-Beclin 1 antibody (M00327-2). Beclin 1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Beclin 1 Antibody (M00327-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-becn1-primary-antibodies-ihc-testing-5.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3) IHC analysis of Beclin 1 using anti-Beclin 1 antibody (M00327-2). Beclin 1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Beclin 1 Antibody (M00327-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-becn1-primary-antibodies-if-testing-6.jpgAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3) IF analysis of Beclin 1 using anti-Beclin 1 antibody (M00327-2). Beclin 1 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Beclin 1 Antibody (M00327-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-2-becn1-primary-antibodies-fcm-testing-7.pngAnti-Beclin 1 Antibody Picoband® (monoclonal, 2D12A3) Flow Cytometry analysis of PC-3 cells using anti-Beclin 1 antibody (M00327-2). Overlay histogram showing PC-3 cells stained with M00327-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Beclin 1 Antibody (M00327-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-maspin-picoband-trade-antibody-m03409-boster.html2026-04-03T05:00:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03409-serpinb5-primary-antibodies-wb-testing-1.jpgAnti-MASPIN Antibody Picoband® (monoclonal, 7G4E1) Western blot analysis of MASPIN using anti-MASPIN antibody (M03409). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MASPIN antigen affinity purified monoclonal antibody (Catalog # M03409) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MASPIN at approximately 42 kDa. The expected band size for MASPIN is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03409-serpinb5-primary-antibodies-ihc-testing-2.jpgAnti-MASPIN Antibody Picoband® (monoclonal, 7G4E1) IHC analysis of MASPIN using anti-MASPIN antibody (M03409). MASPIN was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MASPIN Antibody (M03409) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03409-serpinb5-primary-antibodies-ihc-testing-3.jpgAnti-MASPIN Antibody Picoband® (monoclonal, 7G4E1) IHC analysis of MASPIN using anti-MASPIN antibody (M03409). MASPIN was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MASPIN Antibody (M03409) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03409-serpinb5-primary-antibodies-fcm-testing-4.pngAnti-MASPIN Antibody Picoband® (monoclonal, 7G4E1) Flow Cytometry analysis of SiHa cells using anti-MASPIN antibody (M03409). Overlay histogram showing SiHa cells stained with M03409 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MASPIN Antibody (M03409, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gng4-picoband-trade-antibody-m13925-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13925-gng4-primary-antibodies-wb-testing-1.jpgAnti-GNG4 Antibody Picoband® (monoclonal, 7B6) Western blot analysis of GNG4 using anti-GNG4 antibody (M13925). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNG4 antigen affinity purified monoclonal antibody (Catalog # M13925) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNG4 at approximately 12 kDa. The expected band size for GNG4 is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p23-ptges3-picoband-trade-antibody-m04136-3-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04136-3-ptges3-primary-antibodies-wb-testing-1.jpgAnti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1) Western blot analysis of p23/PTGES3 using anti-p23/PTGES3 antibody (M04136-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p23/PTGES3 antigen affinity purified monoclonal antibody (Catalog # M04136-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p23/PTGES3 at approximately 23 kDa. The expected band size for p23/PTGES3 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04136-3-ptges3-primary-antibodies-if-testing-2.jpgAnti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1) IF analysis of p23/PTGES3 using anti-p23/PTGES3 antibody (M04136-3). p23/PTGES3 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-p23/PTGES3 Antibody (M04136-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04136-3-ptges3-primary-antibodies-fcm-testing-3.pngAnti-p23/PTGES3 Antibody Picoband® (monoclonal, 9D3D1) Flow Cytometry analysis of PC-3 cells using anti-p23/PTGES3 antibody (M04136-3). Overlay histogram showing PC-3 cells stained with M04136-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-p23/PTGES3 Antibody (M04136-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-icam1-picoband-trade-antibody-m00171-3-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-3-icam1-primary-antibodies-wb-testing-1.jpgAnti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) Western blot analysis of ICAM1 using anti-ICAM1 antibody (M00171-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ICAM1 antigen affinity purified monoclonal antibody (Catalog # M00171-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90-110 kDa. The expected band size for ICAM1 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-3-icam1-primary-antibodies-ihc-testing-2.jpgAnti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) IHC analysis of ICAM1 using anti-ICAM1 antibody (M00171-3). ICAM1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ICAM1 Antibody (M00171-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-3-icam1-primary-antibodies-ihc-testing-3.jpgAnti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) IHC analysis of ICAM1 using anti-ICAM1 antibody (M00171-3). ICAM1 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ICAM1 Antibody (M00171-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-3-icam1-primary-antibodies-ihc-testing-4.jpgAnti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) IHC analysis of ICAM1 using anti-ICAM1 antibody (M00171-3). ICAM1 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ICAM1 Antibody (M00171-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-3-icam1-primary-antibodies-ihc-testing-5.jpgAnti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) IHC analysis of ICAM1 using anti-ICAM1 antibody (M00171-3). ICAM1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ICAM1 Antibody (M00171-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-3-icam1-primary-antibodies-fcm-testing-6.pngAnti-ICAM1 Antibody Picoband® (monoclonal, 6F2C3) Flow Cytometry analysis of Caco-2 cells using anti-ICAM1 antibody (M00171-3). Overlay histogram showing Caco-2 cells stained with M00171-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-ICAM1 Antibody (M00171-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-histone-h1-0-h1f0-picoband-trade-antibody-m08821-2-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-wb-testing-1.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) Western blot analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Histone H1.0/H1F0 antigen affinity purified monoclonal antibody (Catalog # M08821-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Histone H1.0/H1F0 at approximately 24 kDa. The expected band size for Histone H1.0/H1F0 is at 24 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-2.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-3.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-4.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of human differentiated adenocarcinoma of the rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-5.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of human Hodgkin's lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-6.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-7.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-8.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-ihc-testing-9.jpgAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) IHC analysis of Histone H1.0/H1F0 using anti-Histone H1.0/H1F0 antibody (M08821-2). Histone H1.0/H1F0 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Histone H1.0/H1F0 Antibody (M08821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08821-2-h1f0-primary-antibodies-fcm-testing-10.pngAnti-Histone H1.0/H1F0 Antibody Picoband® (monoclonal, 5I3E6) Flow Cytometry analysis of SiHa cells using anti-Histone H1.0/H1F0 antibody (M08821-2). Overlay histogram showing SiHa cells stained with M08821-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Histone H1.0/H1F0 Antibody (M08821-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd20-picoband-trade-antibody-m03780-5-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03780-5-cd20-primary-antibodies-wb-testing-1.jpgAnti-CD20 Antibody Picoband® (monoclonal, 4D11) Western blot analysis of CD20 using anti-CD20 antibody (M03780-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD20 antigen affinity purified monoclonal antibody (Catalog # M03780-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD20 at approximately 33 kDa. The expected band size for CD20 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03780-5-cd20-primary-antibodies-ihc-testing-2.jpgAnti-CD20 Antibody Picoband® (monoclonal, 4D11) IHC analysis of CD20 using anti-CD20 antibody (M03780-5). CD20 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD20 Antibody (M03780-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03780-5-cd20-primary-antibodies-ihc-testing-3.jpgAnti-CD20 Antibody Picoband® (monoclonal, 4D11) IHC analysis of CD20 using anti-CD20 antibody (M03780-5). CD20 was detected in a paraffin-embedded section of human lienal rupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD20 Antibody (M03780-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03780-5-cd20-primary-antibodies-if-testing-4_1.jpgAnti-CD20 Antibody Picoband® (monoclonal, 4D11) IF analysis of CD3E and CD20 using anti-CD3E antibody (PB9093) and anti-CD20 antibody (M03780-5). CD3E and CD20 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD3E antibody (PB9093) and mouse anti-CD20 antibody (M03780-5) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135), DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-hnf-4-alpha-picoband-trade-antibody-m00389-2-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-2-hnf4a-primary-antibodies-wb-testing-1.jpgAnti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9) Western blot analysis of HNF-4-alpha using anti-HNF-4-alpha antibody (M00179-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HNF-4-alpha antigen affinity purified monoclonal antibody (Catalog # M00179-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HNF-4-alpha at approximately 53 kDa. The expected band size for HNF-4-alpha is at 53 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-2-hnf4a-primary-antibodies-ihc-testing-2.jpgAnti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9) IHC analysis of HNF-4-alpha using anti-HNF-4-alpha antibody (M00389-2). HNF-4-alpha was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HNF-4-alpha Antibody (M00389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-2-hnf4a-primary-antibodies-ihc-testing-3.jpgAnti-HNF-4-alpha Antibody Picoband® (monoclonal, 6C8E9) IHC analysis of HNF-4-alpha using anti-HNF-4-alpha antibody (M00389-2). HNF-4-alpha was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HNF-4-alpha Antibody (M00389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cytochrome-p450-2e1-cyp2e1-picoband-trade-antibody-m00672-2-boster.html2026-04-06T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00672-2-cyp2e1-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1) Western blot analysis of Cytochrome P450 2E1/CYP2E1 using anti-Cytochrome P450 2E1/CYP2E1 antibody (M00672-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome P450 2E1/CYP2E1 antigen affinity purified monoclonal antibody (Catalog # M00672-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cytochrome P450 2E1/CYP2E1 at approximately 56 kDa. The expected band size for Cytochrome P450 2E1/CYP2E1 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cd55-picoband-trade-antibody-m00910-3-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-3-cd55-primary-antibodies-wb-testing-1.jpgAnti-CD55 Antibody Picoband® (monoclonal, 9F3A1) Western blot analysis of CD55 using anti-CD55 antibody (M00910-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (Catalog # M00910-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-3-cd55-primary-antibodies-ihc-testing-2.jpgAnti-CD55 Antibody Picoband® (monoclonal, 9F3A1) IHC analysis of CD55 using anti-CD55 antibody (M00910-3). CD55 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD55 Antibody (M00910-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-3-cd55-primary-antibodies-if-testing-3.jpgAnti-CD55 Antibody Picoband® (monoclonal, 9F3A1) IF analysis of CD55 using anti-CD55 antibody (M00910-3). CD55 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-CD55 Antibody (M00910-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cd55-picoband-trade-antibody-m00910-4-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-wb-testing-1.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) Western blot analysis of CD55 using anti-CD55 antibody (M00910-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD55 antigen affinity purified monoclonal antibody (Catalog # M00910-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD55 at approximately 70-75 kDa. The expected band size for CD55 is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-ihc-testing-2.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) IHC analysis of CD55 using anti-CD55 antibody (M00910-4). CD55 was detected in a paraffin-embedded section of human cladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD55 Antibody (M00910-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-ihc-testing-3.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) IHC analysis of CD55 using anti-CD55 antibody (M00910-4). CD55 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD55 Antibody (M00910-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-ihc-testing-4.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) IHC analysis of CD55 using anti-CD55 antibody (M00910-4). CD55 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD55 Antibody (M00910-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-ihc-testing-5.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) IHC analysis of CD55 using anti-CD55 antibody (M00910-4). CD55 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD55 Antibody (M00910-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-ihc-testing-6.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) IHC analysis of CD55 using anti-CD55 antibody (M00910-4). CD55 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CD55 Antibody (M00910-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-if-testing-7.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) IF analysis of CD55 using anti-CD55 antibody (M00910-4). CD55 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-CD55 Antibody (M00910-4) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00910-4-cd55-primary-antibodies-if-testing-8.jpgAnti-CD55 Antibody Picoband® (monoclonal, 5B9E1) IF analysis of CD55 using anti-CD55 antibody (M00910-4). CD55 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-CD55 Antibody (M00910-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neuropilin-1-picoband-trade-antibody-m01324-1-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-1-nrp1-primary-antibodies-wb-testing-1.jpgAnti-Neuropilin 1 Antibody Picoband® (monoclonal, 4G3F7) Western blot analysis of Neuropilin 1 using anti-Neuropilin 1 antibody (M01324-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Neuropilin 1 antigen affinity purified monoclonal antibody (Catalog # M01324-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Neuropilin 1 at approximately 120 kDa. The expected band size for Neuropilin 1 is at 120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-1-nrp1-primary-antibodies-if-testing-2.jpgAnti-Neuropilin 1 Antibody Picoband® (monoclonal, 4G3F7) IF analysis of Neuropilin 1 using anti-Neuropilin 1 antibody (M01324-1). Neuropilin 1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Neuropilin 1 Antibody (M01324-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-1-nrp1-primary-antibodies-fcm-testing-3.pngAnti-Neuropilin 1 Antibody Picoband® (monoclonal, 4G3F7) Flow Cytometry analysis of U87 cells using anti-Neuropilin 1 antibody (M01324-1). Overlay histogram showing U87 cells stained with M01324-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Neuropilin 1 Antibody (M01324-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neuropilin-1-picoband-trade-antibody-m01324-2-boster.html2026-03-29T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-2-primary-antibodies-wb-testing-1.jpgAnti-Neuropilin 1 Antibody Picoband® (monoclonal, 8G6G7) Western blot analysis of Neuropilin 1 using anti-Neuropilin 1 antibody (M01324-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Neuropilin 1 antigen affinity purified monoclonal antibody (Catalog # M01324-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Neuropilin 1 at approximately 120 kDa. The expected band size for Neuropilin 1 is at 120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-2-nrp1-primary-antibodies-if-testing-2.jpgAnti-Neuropilin 1 Antibody Picoband® (monoclonal, 8G6G7) IF analysis of Neuropilin 1 using anti-Neuropilin 1 antibody (M01324-2). Neuropilin 1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Neuropilin 1 Antibody (M01324-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-2-nrp1-primary-antibodies-fcm-testing-3.pngAnti-Neuropilin 1 Antibody Picoband® (monoclonal, 8G6G7) Flow Cytometry analysis of U87 cells using anti-Neuropilin 1 antibody (M01324-2). Overlay histogram showing U87 cells stained with M01324-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Neuropilin 1 Antibody (M01324-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pc4-sub1-picoband-trade-antibody-m02698-1-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-1-sub1-primary-antibodies-wb-testing-1.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 2D13E3) Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PC4/SUB1 antigen affinity purified monoclonal antibody (Catalog # M02698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 18 kDa. The expected band size for PC4/SUB1 is at 18 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-1-sub1-primary-antibodies-fcm-testing-2.pngAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 2D13E3) Flow Cytometry analysis of PC-3 cells using anti-PC4/SUB1 antibody (M02698-1). Overlay histogram showing PC-3 cells stained with M02698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PC4/SUB1 Antibody (M02698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pc4-sub1-picoband-trade-antibody-m02698-2-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-wb-testing-1.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PC4/SUB1 antigen affinity purified monoclonal antibody (Catalog # M02698-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 18 kDa. The expected band size for PC4/SUB1 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-2.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-3.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-4.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-5.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-6.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of human poison impregnated thyroid gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-7.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-8.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-2-sub1-primary-antibodies-ihc-testing-9.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 6B5B10) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-2). PC4/SUB1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hgd-picoband-trade-antibody-m01909-1-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01909-1-hgd-primary-antibodies-wb-testing-1.jpgAnti-HGD Antibody Picoband® (monoclonal, 2F11E1) Western blot analysis of HGD using anti-HGD antibody (M01909-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HGD antigen affinity purified monoclonal antibody (Catalog # M01909-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HGD at approximately 50 kDa. The expected band size for HGD is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01909-1-hgd-primary-antibodies-ihc-testing-2.jpgAnti-HGD Antibody Picoband® (monoclonal, 2F11E1) IHC analysis of HGD using anti-HGD antibody (M01909-1). HGD was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HGD Antibody (M01909-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01909-1-hgd-primary-antibodies-fcm-testing-3.pngAnti-HGD Antibody Picoband® (monoclonal, 2F11E1) Flow Cytometry analysis of CACO-2 cells using anti-HGD antibody (M01909-1). Overlay histogram showing CACO-2 cells stained with M01909-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HGD Antibody (M01909-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-prothrombin-picoband-trade-antibody-m00044-3-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00044-3-prothrombin-primary-antibodies-wb-testing-1.jpgAnti-Prothrombin Antibody Picoband® (monoclonal, 5B6G1) Western blot analysis of Prothrombin using anti-Prothrombin antibody (M00044-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Prothrombin antigen affinity purified monoclonal antibody (Catalog # M00044-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Prothrombin at approximately 90 kDa. The expected band size for Prothrombin is at 90 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sod2-picoband-trade-antibody-m00349-3-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-3-sod2-primary-antibodies-wb-testing-1.jpgAnti-SOD2 Antibody Picoband® (monoclonal, 2B12B1) Western blot analysis of SOD2 using anti-SOD2 antibody (M00349-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SOD2 antigen affinity purified monoclonal antibody (Catalog # M00349-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 25 kDa. The expected band size for SOD2 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-3-sod2-primary-antibodies-ihc-testing-2.jpgAnti-SOD2 Antibody Picoband® (monoclonal, 2B12B1) IHC analysis of SOD2 using anti-SOD2 antibody (M00349-3). SOD2 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SOD2 Antibody (M00349-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-3-sod2-primary-antibodies-ihc-testing-3.jpgAnti-SOD2 Antibody Picoband® (monoclonal, 2B12B1) IHC analysis of SOD2 using anti-SOD2 antibody (M00349-3). SOD2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SOD2 Antibody (M00349-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-3-sod2-primary-antibodies-ihc-testing-4.jpgAnti-SOD2 Antibody Picoband® (monoclonal, 2B12B1) IHC analysis of SOD2 using anti-SOD2 antibody (M00349-3). SOD2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SOD2 Antibody (M00349-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-poliovirus-receptor-pvr-picoband-trade-antibody-m00664-1-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-1-pvr-primary-antibodies-wb-testing-1.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 9B9F1) Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Poliovirus Receptor/PVR antigen affinity purified monoclonal antibody (Catalog # M00664-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70-80 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-1-pvr-primary-antibodies-ihc-testing-2.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 9B9F1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-1). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-1-pvr-primary-antibodies-ihc-testing-3.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 9B9F1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-1). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-1-pvr-primary-antibodies-ihc-testing-4.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 9B9F1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-1). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-1-pvr-primary-antibodies-ihc-testing-5.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 9B9F1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-1). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-1-pvr-primary-antibodies-ihc-testing-6.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 9B9F1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-1). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-1-pvr-primary-antibodies-ihc-testing-7.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 9B9F1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-1). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-poliovirus-receptor-pvr-picoband-trade-antibody-m00664-2-boster.html2026-03-27T05:07:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-wb-testing-1.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Poliovirus Receptor/PVR antigen affinity purified monoclonal antibody (Catalog # M00664-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70-80 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-ihc-testing-2.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-ihc-testing-3.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-ihc-testing-4.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-ihc-testing-5.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-ihc-testing-6.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-ihc-testing-7.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-ihc-testing-8.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-2). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-2-pvr-primary-antibodies-fcm-testing-9.pngAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 5I13D1) Flow Cytometry analysis of PC-3 cells using anti-Poliovirus Receptor/PVR antibody (M00664-2). Overlay histogram showing PC-3 cells stained with M00664-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Poliovirus Receptor/PVR Antibody (M00664-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-poliovirus-receptor-pvr-picoband-trade-antibody-m00664-3-boster.html2026-03-17T05:13:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-3-pvr-primary-antibodies-wb-testing-1.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9) Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Poliovirus Receptor/PVR antigen affinity purified monoclonal antibody (Catalog # M00664-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70-80 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-3-pvr-primary-antibodies-ihc-testing-2.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-3). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-3-pvr-primary-antibodies-fcm-testing-3.pngAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9) Flow Cytometry analysis of PC-3 cells using anti-Poliovirus Receptor/PVR antibody (M00664-3). Overlay histogram showing PC-3 cells stained with M00664-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Poliovirus Receptor/PVR Antibody (M00664-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00664-3-pvr-primary-antibodies-ihc-testing-4.jpgAnti-Poliovirus Receptor/PVR Antibody Picoband® (monoclonal, 3B11E9) IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (M00664-3). Poliovirus Receptor/PVR was detected in a paraffin-embedded section of mouse liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Poliovirus Receptor/PVR Antibody (M00664-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/picokine-elisa-kits/human-caspr2-picokine-elisa-kit-ek2169-boster.html2026-03-10T04:33:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2169.pngHuman CASPR2 ELISA Kit PicoKine®Human CASPR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cntfr-picokine-elisa-kit-ek2170-boster.html2026-03-10T04:33:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2170.pngHuman CNTFR ELISA Kit PicoKine®Human CNTFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd131-picokine-elisa-kit-ek2171-boster.html2026-03-10T04:33:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2171.pngHuman CD131 ELISA Kit PicoKine®Human CD131 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-crtam-picokine-elisa-kit-ek2172-boster.html2026-03-10T04:33:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2172.pngHuman CRTAM ELISA Kit PicoKine®Human CRTAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cspg5-picokine-elisa-kit-ek2173-boster.html2026-03-10T04:33:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2173.pngHuman CSPG5 ELISA Kit PicoKine®Human CSPG5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gm-csfr-picokine-elisa-kit-ek2174-boster.html2026-03-10T04:33:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2174.pngHuman GM-CSFR ELISA Kit PicoKine®Human GM-CSFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-gata4-q258-antibody-a00499-3-boster.html2026-03-10T04:33:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-pap-acp3-picoband-trade-antibody-a02082-2-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02082-2-acpp-primary-antibodies-wb-testing-1.jpgAnti-PAP/ACP3 Antibody Picoband® Western blot analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAP/ACP3 antigen affinity purified polyclonal antibody (Catalog # A02082-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAP/ACP3 at approximately 45-50 kDa. The expected band size for PAP/ACP3 is at 45 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02082-2-acpp-primary-antibodies-ihc-testing-2.jpgAnti-PAP/ACP3 Antibody Picoband® IHC analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). PAP/ACP3 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAP/ACP3 Antibody (A02082-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02082-2-acpp-primary-antibodies-ihc-testing-3.jpgAnti-PAP/ACP3 Antibody Picoband® IHC analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). PAP/ACP3 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAP/ACP3 Antibody (A02082-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02082-2-acpp-primary-antibodies-ihc-testing-4.jpgAnti-PAP/ACP3 Antibody Picoband® IHC analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). PAP/ACP3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAP/ACP3 Antibody (A02082-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02082-2-acpp-primary-antibodies-if-testing-5.jpgAnti-PAP/ACP3 Antibody Picoband® IF analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (A02082-2). PAP/ACP3 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAP/ACP3 Antibody (A02082-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02082-2-acpp-primary-antibodies-fcm-testing-6.pngAnti-PAP/ACP3 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-PAP/ACP3 antibody (A02082-2). Overlay histogram showing HL-60 cells stained with A02082-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAP/ACP3 Antibody (A02082-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ms2-adam8-picoband-trade-antibody-a04306-4-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04306-4-adam8-primary-antibodies-wb-testing-1.jpgAnti-MS2/ADAM8 Antibody Picoband® Western blot analysis of MS2/ADAM8 using anti-MS2/ADAM8 antibody (A04306-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MS2/ADAM8 antigen affinity purified polyclonal antibody (Catalog # A04306-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MS2/ADAM8 at approximately 80 kDa. The expected band size for MS2/ADAM8 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04306-4-adam8-primary-antibodies-fcm-testing-2.pngAnti-MS2/ADAM8 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-MS2/ADAM8 antibody (A04306-4). Overlay histogram showing U87 cells stained with A04306-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MS2/ADAM8 Antibody (A04306-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-adam10-picoband-trade-antibody-a00566-5-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-5-adam10-primary-antibodies-wb-testing-1.jpgAnti-ADAM10 Antibody Picoband® Western blot analysis of ADAM10 using anti-ADAM10 antibody (A00566-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM10 antigen affinity purified polyclonal antibody (Catalog # A00566-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM10 at approximately 70 kDa. The expected band size for ADAM10 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00566-5-adam10-primary-antibodies-fcm-testing-2.pngAnti-ADAM10 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-ADAM10 antibody (A00566-5). Overlay histogram showing U937 cells stained with A00566-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAM10 Antibody (A00566-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-alas2-asb-picoband-trade-antibody-a02525-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02525-1-alas2-primary-antibodies-wb-testing-1.jpgAnti-ALAS2/ASB Antibody Picoband® Western blot analysis of ALAS2/ASB using anti-ALAS2/ASB antibody (A02525-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALAS2/ASB antigen affinity purified polyclonal antibody (Catalog # A02525-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALAS2/ASB at approximately 65 kDa. The expected band size for ALAS2/ASB is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02525-1-alas2-primary-antibodies-if-testing-2.jpgAnti-ALAS2/ASB Antibody Picoband® IF analysis of ALAS2/ASB using anti-ALAS2/ASB antibody (A02525-1). ALAS2/ASB was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALAS2/ASB Antibody (A02525-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02525-1-alas2-primary-antibodies-fcm-testing-3.pngAnti-ALAS2/ASB Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-ALAS2/ASB antibody (A02525-1). Overlay histogram showing K562 cells stained with A02525-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALAS2/ASB Antibody (A02525-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-apaf1-picoband-trade-antibody-a00889-2-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00889-2-apaf1-primary-antibodies-wb-testing-1.jpgAnti-APAF1 Antibody Picoband® Western blot analysis of APAF1 using anti-APAF1 antibody (A00889-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APAF1 antigen affinity purified polyclonal antibody (Catalog # A00889-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APAF1 at approximately 135 kDa. The expected band size for APAF1 is at 135 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00889-2-apaf1-primary-antibodies-fcm-testing-2.pngAnti-APAF1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-APAF1 antibody (A00889-2). Overlay histogram showing U937 cells stained with A00889-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APAF1 Antibody (A00889-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ate1-picoband-trade-antibody-a07459-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07459-1-ate1-primary-antibodies-wb-testing-1.jpgAnti-ATE1 Antibody Picoband® Western blot analysis of ATE1 using anti-ATE1 antibody (A07459-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATE1 antigen affinity purified polyclonal antibody (Catalog # A07459-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATE1 at approximately 59 kDa. The expected band size for ATE1 is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-aup1-picoband-trade-antibody-a08937-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08937-1-aup1-primary-antibodies-wb-testing-1.jpgAnti-AUP1 Antibody Picoband® Western blot analysis of AUP1 using anti-AUP1 antibody (A08937-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AUP1 antigen affinity purified polyclonal antibody (Catalog # A08937-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AUP1 at approximately 42 kDa. The expected band size for AUP1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08937-1-aup1-primary-antibodies-fcm-testing-2.pngAnti-AUP1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-AUP1 antibody (A08937-1). Overlay histogram showing U937 cells stained with A08937-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AUP1 Antibody (A08937-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ccr2-picoband-trade-antibody-a00158-7-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00158-7-ccr2-primary-antibodies-wb-testing-1.jpgAnti-CCR2 Antibody Picoband® Western blot analysis of CCR2 using anti-CCR2 antibody (A00158-7). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified polyclonal antibody (Catalog # A00158-7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR2 at approximately 50 kDa. The expected band size for CCR2 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00158-7-ccr2-primary-antibodies-ihc-testing-2.jpgAnti-CCR2 Antibody Picoband® IHC analysis of CCR2 using anti-CCR2 antibody (A00158-7). CCR2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCR2 Antibody (A00158-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00158-7-ccr2-primary-antibodies-ihc-testing-3.jpgAnti-CCR2 Antibody Picoband® IHC analysis of CCR2 using anti-CCR2 antibody (A00158-7). CCR2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCR2 Antibody (A00158-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00158-7-ccr2-primary-antibodies-ihc-testing-4.jpgAnti-CCR2 Antibody Picoband® IHC analysis of CCR2 using anti-CCR2 antibody (A00158-7). CCR2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCR2 Antibody (A00158-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ccr7-picoband-trade-antibody-a00390-2-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00390-2-ccr7-primary-antibodies-ihc-testing-1.jpgAnti-CCR7 Antibody IHC analysis of CCR7 using anti-CCR7 antibody (A00390-2). CCR7 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCR7 Antibody (A00390-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00390-2-ccr7-primary-antibodies-fcm-testing-2.pngAnti-CCR7 Antibody Flow Cytometry analysis of U20S cells using anti-CCR7 antibody (A00390-2). Overlay histogram showing U20S cells stained with A00390-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR7 Antibody (A00390-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd68-picoband-trade-antibody-a00602-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-ihc-testing-1_1_1.jpgAnti-CD68 Antibody IHC analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-ihc-testing-2_1.pngAnti-CD68 Antibody IHC analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-ihc-testing-3_1.pngAnti-CD68 Antibody IHC analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-ihc-testing-4_1.pngAnti-CD68 Antibody IHC analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-ihc-testing-5_1.pngAnti-CD68 Antibody IHC analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-if-testing-1.jpgAnti-CD68 AntibodyIF analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-if-testing-6_1.jpgAnti-CD68 Antibody IF analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-if-testing-7_1.pngAnti-CD68 Antibody IF analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight 488 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-if-testing-9_1.pngAnti-CD68 Antibody IF analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human normal placenta and preterm placentar tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)(BA1148) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-cd68-primary-antibodies-if-testing-8_1.pngAnti-CD68 Antibody IF analysis of CD68 using anti-CD68 antibody (A00602-1). CD68 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight 488 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-13567_2025_1630_fig6_html.pngAnti-CD68 AntibodyCoordination of the hepatocyte IL-1β transcription-translation-mature tRNAome axis by monocytes . A Representative images of monocytes (light microscopy and hematoxylin staining). The identification of monocytes was confirmed by a rabbit anti-CD68 polyclonal antibody (green), and the results were compared with those of DEFs. DAPI was used for nuclear staining. B The impact of monocytes on the transcription of proinflammatory cytokines in infected hepatocytes. The hepatocytes were cocultured with a total of 5.0 × 10 6 PMBCs per well or with the corresponding monocytes derived from the same number of PBMCs. A total of 2.0 × 10 7 copies per well of DHAV were subsequently used. Samples were collected at 24 hpc. RT‒qPCR was used to quantify the expression of IL-1β, IL-6, and TNF-α compared with that in uninfected DPHs ( n = 3). Statistical significance was determined via unpaired t tests; *, p < 0.05; **, p < 0.01; ***, p < 0.001. C The impact of PBMCs or monocytes on the 23 selected tRNAs in infected DPHs. The tRNA data were normalized to those of the uninfected DPHs ( n = 3). D Western blotting of the IL-1β protein in infected DPHs cocultured with PBMCs or monocytes at 24 hpc, with the same number of cells used as in panel B. The gray value of the IL-1β protein was then quantitatively evaluated via ImageJ software. E Dynamic changes in the expression of IL-1β mRNA in DHAV-infected DPHs cocultured with monocytes from 12 to 48 hpc ( n = 3). DHAV-infected DPHs lacking monocyte coculture were used as a control, and uninfected DPHs were used as a negative control for gene normalization. F Dynamic changes in the 23 selected tRNAs in DPHs cocultured with monocytes. The tRNA data were normalized to those of the uninfected DPHs ( n = 3). G Western blotting of IL-1β in infected DPHs during different durations of coculture with monocytes (12 hpc, 24 hpc, and 48 hpc). The amount of IL-1β protein was determined by analysing the gray value via ImageJ software. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13567-025-01630-9'>41108011</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-fphar-16-1554945-g010.jpgAnti-CD68 AntibodyTUDCA promoted macrophages shift to M2-like phenotype and impacted endogenous NSCs morphology. (A) Immunofluorescent staining of M1-like (iNOS, red) and M2-like macrophages (CD163, green) at the margin of the lesion site at day 7 after SCI. (B, C) Quantification the number of iNOS + or CD163 cells. (D) Macrophages (CD68, red) and endogenous NSCs (Nestin, green) at the margin of the lesion site at day 7 after SCI. All experiments were performed in triplicated and data were presented means ± SEM, n = 3 per group. **P < 0.01, ***P < 0.001 . Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-fphar-16-1554945-g009.jpgAnti-CD68 AntibodyTUDCA regulated macrophages and reactive astrocytes distribution. Co-immunofluorescence images showed macrophages (CD68, red) and reactive astrocytes (GFAP, green) at day 3 and day 7 after SCI. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00602-1-fphar-16-1554945-g008.jpgAnti-CD68 AntibodyTUDCA regulated macrophages and endogenous NSCs distribution. Co-immunofluorescence images showed macrophages (CD68, red) and endogenous NSCs (Nestin, green) at day 3 and day 7 after SCI. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a> https://www.bosterbio.com/products/primary-antibodies/anti-cd101-picoband-trade-antibody-a07106-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07106-1-cd101-primary-antibodies-ihc-testing-1.jpgAnti-CD101 Antibody IHC analysis of CD101 using anti-CD101 antibody (A07106-1). CD101 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07106-1-cd101-primary-antibodies-ihc-testing-2.jpgAnti-CD101 Antibody IHC analysis of CD101 using anti-CD101 antibody (A07106-1). CD101 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07106-1-cd101-primary-antibodies-ihc-testing-3.jpgAnti-CD101 Antibody IHC analysis of CD101 using anti-CD101 antibody (A07106-1). CD101 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07106-1-cd101-primary-antibodies-ihc-testing-4.jpgAnti-CD101 Antibody IHC analysis of CD101 using anti-CD101 antibody (A07106-1). CD101 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD101 Antibody (A07106-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cenpi-picoband-trade-antibody-a10263-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10263-1-cenpi-primary-antibodies-wb-testing-1.jpgAnti-CENPI Antibody Picoband® Western blot analysis of CENPI using anti-CENPI antibody (A10263-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPI antigen affinity purified polyclonal antibody (Catalog # A10263-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPI at approximately 60 kDa. The expected band size for CENPI is at 60 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10263-1-cenpi-primary-antibodies-fcm-testing-2.pngAnti-CENPI Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CENPI antibody (A10263-1). Overlay histogram showing U937 cells stained with A10263-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CENPI Antibody (A10263-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-choline-acetyltransferase-chat-picoband-trade-antibody-a01192-6-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-6-chat-primary-antibodies-wb-testing-1.jpgAnti-Choline Acetyltransferase/CHAT Antibody Picoband® Western blot analysis of Choline Acetyltransferase/CHAT using anti-Choline Acetyltransferase/CHAT antibody (A01192-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Choline Acetyltransferase/CHAT antigen affinity purified polyclonal antibody (Catalog # A01192-6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Choline Acetyltransferase/CHAT at approximately 71 kDa. The expected band size for Choline Acetyltransferase/CHAT is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01192-6-chat-primary-antibodies-fcm-testing-2.pngAnti-Choline Acetyltransferase/CHAT Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-Choline Acetyltransferase/CHAT antibody (A01192-6). Overlay histogram showing U937 cells stained with A01192-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Choline Acetyltransferase/CHAT Antibody (A01192-6, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-e2f3-picoband-trade-antibody-a03068-4-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03068-4-e2f3-primary-antibodies-wb-testing-1.jpgAnti-E2F3 Antibody Picoband® Western blot analysis of E2F3 using anti-E2F3 antibody (A03068-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F3 antigen affinity purified polyclonal antibody (Catalog # A03068-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F3 at approximately 45 kDa. The expected band size for E2F3 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03068-4-e2f3-primary-antibodies-ihc-testing-2.jpgAnti-E2F3 Antibody Picoband® IHC analysis of E2F3 using anti-E2F3 antibody (A03068-4). E2F3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E2F3 Antibody (A03068-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03068-4-e2f3-primary-antibodies-fcm-testing-3.pngAnti-E2F3 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-E2F3 antibody (A03068-4). Overlay histogram showing HL-60 cells stained with A03068-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-E2F3 Antibody (A03068-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pp11-endou-picoband-trade-antibody-a07350-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07350-1-endou-primary-antibodies-wb-testing-1.jpgAnti-PP11/ENDOU Antibody Picoband® Western blot analysis of PP11/ENDOU using anti-PP11/ENDOU antibody (A07350-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PP11/ENDOU antigen affinity purified polyclonal antibody (Catalog # A07350-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PP11/ENDOU at approximately 47 kDa. The expected band size for PP11/ENDOU is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07350-1-endou-primary-antibodies-ihc-testing-2.jpgAnti-PP11/ENDOU Antibody Picoband® IHC analysis of PP11/ENDOU using anti-PP11/ENDOU antibody (A07350-1). PP11/ENDOU was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PP11/ENDOU Antibody (A07350-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07350-1-endou-primary-antibodies-ihc-testing-3.jpgAnti-PP11/ENDOU Antibody Picoband® IHC analysis of PP11/ENDOU using anti-PP11/ENDOU antibody (A07350-1). PP11/ENDOU was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PP11/ENDOU Antibody (A07350-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07350-1-endou-primary-antibodies-if-testing-4.jpgAnti-PP11/ENDOU Antibody Picoband® IF analysis of PP11/ENDOU using anti-PP11/ENDOU antibody (A07350-1). PP11/ENDOU was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PP11/ENDOU Antibody (A07350-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07350-1-endou-primary-antibodies-fcm-testing-5.pngAnti-PP11/ENDOU Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-PP11/ENDOU antibody (A07350-1). Overlay histogram showing U87 cells stained with A07350-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PP11/ENDOU Antibody (A07350-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-enpp1-pc1-picoband-trade-antibody-a01342-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01342-1-enpp1-primary-antibodies-wb-testing-1.jpgAnti-ENPP1/PC1 Antibody Picoband® Western blot analysis of ENPP1/PC1 using anti-ENPP1/PC1 antibody (A01342-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENPP1/PC1 antigen affinity purified polyclonal antibody (Catalog # A01342-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENPP1/PC1 at approximately 130-150 kDa. The expected band size for ENPP1/PC1 is at 105 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01342-1-enpp1-primary-antibodies-ihc-testing-2.jpgAnti-ENPP1/PC1 Antibody Picoband® IHC analysis of ENPP1/PC1 using anti-ENPP1/PC1 antibody (A01342-1). ENPP1/PC1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENPP1/PC1 Antibody (A01342-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01342-1-enpp1-primary-antibodies-ihc-testing-3.jpgAnti-ENPP1/PC1 Antibody Picoband® IHC analysis of ENPP1/PC1 using anti-ENPP1/PC1 antibody (A01342-1). ENPP1/PC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENPP1/PC1 Antibody (A01342-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01342-1-enpp1-primary-antibodies-ihc-testing-4.jpgAnti-ENPP1/PC1 Antibody Picoband® IHC analysis of ENPP1/PC1 using anti-ENPP1/PC1 antibody (A01342-1). ENPP1/PC1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENPP1/PC1 Antibody (A01342-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01342-1-enpp1-primary-antibodies-fcm-testing-5.pngAnti-ENPP1/PC1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-ENPP1/PC1 antibody (A01342-1). Overlay histogram showing HepG2 cells stained with A01342-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENPP1/PC1 Antibody (A01342-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eif5a-picoband-trade-antibody-a01727-4-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-wb-testing-1.jpgAnti-EIF5A Antibody Picoband® Western blot analysis of EIF5A using anti-EIF5A antibody (A01727-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF5A antigen affinity purified polyclonal antibody (Catalog # A01727-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF5A at approximately 18 kDa. The expected band size for EIF5A is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-wb-testing-2.jpgAnti-EIF5A Antibody Picoband® Western blot analysis of EIF5A using anti-EIF5A antibody (A01727-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissuelysates, Lane 3: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF5A antigen affinity purified polyclonal antibody (Catalog # A01727-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF5A at approximately 18 kDa. The expected band size for EIF5A is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-ihc-testing-3.jpgAnti-EIF5A Antibody Picoband® IHC analysis of EIF5A using anti-EIF5A antibody (A01727-4). EIF5A was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF5A Antibody (A01727-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-ihc-testing-4.jpgAnti-EIF5A Antibody Picoband® IHC analysis of EIF5A using anti-EIF5A antibody (A01727-4). EIF5A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF5A Antibody (A01727-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-ihc-testing-5.jpgAnti-EIF5A Antibody Picoband® IHC analysis of EIF5A using anti-EIF5A antibody (A01727-4). EIF5A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF5A Antibody (A01727-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-if-testing-6.jpgAnti-EIF5A Antibody Picoband® IF analysis of EIF5A using anti-EIF5A antibody (A01727-4). EIF5A was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EIF5A Antibody (A01727-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-fcm-testing-7.pngAnti-EIF5A Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-EIF5A antibody (A01727-4). Overlay histogram showing K562 cells stained with A01727-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF5A Antibody (A01727-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01727-4-eif5a-primary-antibodies-fcm-testing-8.pngAnti-EIF5A Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-EIF5A antibody (A01727-4). Overlay histogram showing RH35 cells stained with A01727-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF5A Antibody (A01727-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eph-receptor-a3-epha3-picoband-trade-antibody-a02872-1-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02872-1-epha3-primary-antibodies-wb-testing-1.jpgAnti-Eph receptor A3/EPHA3 Antibody Picoband® Western blot analysis of Eph Receptor A3/EPHA3 using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph Receptor A3/EPHA3 antigen affinity purified polyclonal antibody (Catalog # A02872-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph Receptor A3/EPHA3 at approximately 135 kDa. The expected band size for Eph Receptor A3/EPHA3 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02872-1-epha3-primary-antibodies-ihc-testing-2.jpgAnti-Eph receptor A3/EPHA3 Antibody Picoband® IHC analysis of Eph Receptor A3/EPHA3 using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Eph Receptor A3/EPHA3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Eph Receptor A3/EPHA3 Antibody (A02872-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02872-1-epha3-primary-antibodies-if-testing-3.jpgAnti-Eph receptor A3/EPHA3 Antibody Picoband® IF analysis of Eph Receptor A3/EPHA3 using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Eph Receptor A3/EPHA3 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Eph Receptor A3/EPHA3 Antibody (A02872-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02872-1-epha3-primary-antibodies-fcm-testing-4.pngAnti-Eph receptor A3/EPHA3 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Overlay histogram showing PC-3 cells stained with A02872-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Eph Receptor A3/EPHA3 Antibody (A02872-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ttc11-fis1-picoband-trade-antibody-a01932-3-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-wb-testing-1.jpgAnti-TTC11/FIS1 Antibody Picoband® Western blot analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC11/FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC11/FIS1 at approximately 17 kDa. The expected band size for TTC11/FIS1 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-13287_2025_4422_fig3_html.pngAnti-TTC11/FIS1 Antibody Picoband®DMOG treatment ameliorates mitochondrial dysfunction and enhances mitophagy and ATP production in aged MSCs. ( A ) Representative JC-1 fluorescence images showing mitochondrial membrane potential in different groups: CCCP (positive control for mitochondrial depolarization), P5 MSCs, P5 + H₂O₂-treated MSCs, P5 + H₂O₂+DMOG-treated MSCs, P15 MSCs, and P15 + DMOG-treated MSCs. JC-1 red fluorescence indicates high mitochondrial membrane potential, whereas green fluorescence indicates depolarized mitochondria. Scale bar = 100 μm. ( B ) Representative MitoSox Red fluorescence images showing mitochondrial ROS levels in different treatment groups. Scale bar = 10 μm. ( C , D ) Quantitative analysis of the mitochondrial membrane potential ( C ) and mitochondrial ROS levels ( D ). ( E ) Western blot analysis showing the expression levels of mitophagy-related proteins (MFN1, MFN2, and Fis1) in P5 and P15 MSCs with or without H₂O₂ and DMOG treatment. GAPDH was used as a loading control. ( F ) Representative confocal images of co-staining with LysoTracker Red (lysosomes) and MitoTracker Green (mitochondria) in MSCs under different conditions. Scale bar = 10 μm. ( G ) Quantitative analysis of the number of mitophagosomes per cell in the different groups. ( H ) Quantitative analysis of ATP production per cell in the different groups. Data are expressed as the mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01. *** p < 0.001. Full-length blots are presented in Supplementary Materials - WB Raw Data Full size image Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-025-04422-2'>40457488</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-ihc-testing-2.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). TTC11/FIS1 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-ihc-testing-3.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). TTC11/FIS1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-ihc-testing-4.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). TTC11/FIS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-ihc-testing-5.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). TTC11/FIS1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-ihc-testing-6.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). TTC11/FIS1 was detected in a paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-ihc-testing-7.jpgAnti-TTC11/FIS1 Antibody Picoband® IHC analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). TTC11/FIS1 was detected in a paraffin-embedded section of human poison impregnated thyroid gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01932-3-fis1-primary-antibodies-if-testing-8.jpgAnti-TTC11/FIS1 Antibody Picoband® IF analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (A01932-3). TTC11/FIS1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TTC11/FIS1 Antibody (A01932-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-gpcr-tgr5-gpbar1-picoband-trade-antibody-a01958-2-boster.html2026-03-17T05:13:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01958-2-gpbar1-primary-antibodies-wb-testing-1.jpgAnti-GPCR TGR5/GPBAR1 Antibody Picoband® Western blot analysis of GPCR TGR5/GPBAR1 using anti-GPCR TGR5/GPBAR1 antibody (A01958-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR TGR5/GPBAR1 antigen affinity purified polyclonal antibody (Catalog # A01958-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR TGR5/GPBAR1 at approximately 45 kDa. The expected band size for GPCR TGR5/GPBAR1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01958-2-gpbar1-primary-antibodies-fcm-testing-2.pngAnti-GPCR TGR5/GPBAR1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-GPCR TGR5/GPBAR1 antibody (A01958-2). Overlay histogram showing THP-1 cells stained with A01958-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GPCR TGR5/GPBAR1 Antibody (A01958-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hcn4-picoband-trade-antibody-a02235-1-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02235-1-hcn4-primary-antibodies-wb-testing-1.jpgAnti-HCN4 Antibody Picoband® Western blot analysis of HCN4 using anti-HCN4 antibody (A02235-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCN4 antigen affinity purified polyclonal antibody (Catalog # A02235-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN4 at approximately 140 kDa. The expected band size for HCN4 is at 129 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02235-1-hcn4-primary-antibodies-fcm-testing-2.pngAnti-HCN4 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-HCN4 antibody (A02235-1). Overlay histogram showing MCF-7 cells stained with A02235-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HCN4 Antibody (A02235-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ifitm1-picoband-trade-antibody-a02633-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02633-2-ifitm1-primary-antibodies-wb-testing-1.jpgAnti-IFITM1 Antibody Picoband® Western blot analysis of IFITM1 using anti-IFITM1 antibody (A02633-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFITM1 antigen affinity purified polyclonal antibody (Catalog # A02633-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFITM1 at approximately 15 kDa. The expected band size for IFITM1 is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-lsr-picoband-trade-antibody-a02742-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02742-2-lsr-primary-antibodies-wb-testing-1.jpgAnti-LSR Antibody Picoband® Western blot analysis of LSR using anti-LSR antibody (A02742). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSR antigen affinity purified polyclonal antibody (Catalog # A02742) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LSR at approximately 65 kDa. The expected band size for LSR is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02742-2-lsr-primary-antibodies-fcm-testing-2.pngAnti-LSR Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-LSR antibody (A02742-2). Overlay histogram showing MCF-7 cells stained with A02742-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LSR Antibody (A02742-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd204-msr1-picoband-trade-antibody-a02349-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02349-2-msr1-primary-antibodies-wb-testing-1_1.jpgAnti-CD204/Msr1 Antibody Picoband® Western blot analysis of CD204/Msr1 using anti-CD204/Msr1 antibody (A02349-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD204/Msr1 antigen affinity purified polyclonal antibody (Catalog # A02349-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD204/Msr1 at approximately 75-90 kDa. The expected band size for CD204/Msr1 is at 71 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02349-2-msr1-primary-antibodies-fcm-testing-2.jpgAnti-CD204/Msr1 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-CD204/Msr1 antibody (A02349-2). Overlay histogram showing RAW264.7 cells stained with A02349-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD204/Msr1 Antibody (A02349-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-muc5b-picoband-trade-antibody-a00719-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00719-2-muc5b-primary-antibodies-ihc-testing-1.jpgAnti-MUC5B Antibody IHC analysis of MUC5B using anti-MUC5B antibody (A00719-2). MUC5B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC5B Antibody (A00719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00719-2-muc5b-primary-antibodies-ihc-testing-2.jpgAnti-MUC5B Antibody IHC analysis of MUC5B using anti-MUC5B antibody (A00719-2). MUC5B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC5B Antibody (A00719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-neurotensin-receptor-1-ntsr1-picoband-trade-antibody-a04610-4-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04610-4-ntsr1-primary-antibodies-wb-testing-1.jpgAnti-Neurotensin Receptor 1/NTSR1 Antibody Picoband® Western blot analysis of Neurotensin Receptor 1/NTSR1 using anti-Neurotensin Receptor 1/NTSR1 antibody (A04610-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurotensin Receptor 1/NTSR1 antigen affinity purified polyclonal antibody (Catalog # A04610-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurotensin Receptor 1/NTSR1 at approximately 43 kDa. The expected band size for Neurotensin Receptor 1/NTSR1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04610-4-ntsr1-primary-antibodies-fcm-testing-2.pngAnti-Neurotensin Receptor 1/NTSR1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Neurotensin Receptor 1/NTSR1 antibody (A04610-4). Overlay histogram showing THP-1 cells stained with A04610-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Neurotensin Receptor 1/NTSR1 Antibody (A04610-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-palb2-picoband-trade-antibody-a00639-1-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00639-1-palb2-primary-antibodies-wb-testing-1.jpgAnti-PALB2 Antibody Picoband® Western blot analysis of PALB2 using anti-PALB2 antibody (A00639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PALB2 antigen affinity purified polyclonal antibody (Catalog # A00639-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PALB2 at approximately 130 kDa. The expected band size for PALB2 is at 130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00639-1-palb2-primary-antibodies-fcm-testing-2.pngAnti-PALB2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PALB2 antibody (A00639-1). Overlay histogram showing THP-1 cells stained with A00639-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PALB2 Antibody (A00639-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pleckstrin-plek-picoband-trade-antibody-a06656-3-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06656-3-plek-primary-antibodies-wb-testing-1.jpgAnti-Pleckstrin/PLEK Antibody Picoband® Western blot analysis of Pleckstrin/PLEK using anti-Pleckstrin/PLEK antibody (A06656-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleckstrin/PLEK antigen affinity purified polyclonal antibody (Catalog # A06656-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleckstrin/PLEK at approximately 40 kDa. The expected band size for Pleckstrin/PLEK is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06656-3-plek-primary-antibodies-ihc-testing-2.jpgAnti-Pleckstrin/PLEK Antibody Picoband® IHC analysis of Pleckstrin/PLEK using anti-Pleckstrin/PLEK antibody (A06656-3). Pleckstrin/PLEK was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Pleckstrin/PLEK Antibody (A06656-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06656-3-plek-primary-antibodies-fcm-testing-3.pngAnti-Pleckstrin/PLEK Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-Pleckstrin/PLEK antibody (A06656-3). Overlay histogram showing HL-60 cells stained with A06656-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Pleckstrin/PLEK Antibody (A06656-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-plp2-picoband-trade-antibody-a06255-1-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-wb-testing-1.jpgAnti-PLP2 Antibody Picoband® Western blot analysis of PLP2 using anti-PLP2 antibody (A06255-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO320 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLP2 antigen affinity purified polyclonal antibody (Catalog # A06255-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLP2 at approximately 17 kDa. The expected band size for PLP2 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-ihc-testing-2.jpgAnti-PLP2 Antibody Picoband® IHC analysis of PLP2 using anti-PLP2 antibody (A06255-1). PLP2 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLP2 Antibody (A06255-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-ihc-testing-3.jpgAnti-PLP2 Antibody Picoband® IHC analysis of PLP2 using anti-PLP2 antibody (A06255-1). PLP2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLP2 Antibody (A06255-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-ihc-testing-4.jpgAnti-PLP2 Antibody Picoband® IHC analysis of PLP2 using anti-PLP2 antibody (A06255-1). PLP2 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLP2 Antibody (A06255-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-ihc-testing-5.jpgAnti-PLP2 Antibody Picoband® IHC analysis of PLP2 using anti-PLP2 antibody (A06255-1). PLP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLP2 Antibody (A06255-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-ihc-testing-6.jpgAnti-PLP2 Antibody Picoband® IHC analysis of PLP2 using anti-PLP2 antibody (A06255-1). PLP2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLP2 Antibody (A06255-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-ihc-testing-7.jpgAnti-PLP2 Antibody Picoband® IHC analysis of PLP2 using anti-PLP2 antibody (A06255-1). PLP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLP2 Antibody (A06255-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06255-1-plp2-primary-antibodies-fcm-testing-8.pngAnti-PLP2 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-PLP2 antibody (A06255-1). Overlay histogram showing Daudi cells stained with A06255-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLP2 Antibody (A06255-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-prkra-picoband-trade-antibody-a02744-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02744-2-prkra-primary-antibodies-wb-testing-1.jpgAnti-PRKRA Antibody Picoband® Western blot analysis of PRKRA using anti-PRKRA antibody (A02744-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKRA antigen affinity purified polyclonal antibody (Catalog # A02744-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKRA at approximately 32 kDa. The expected band size for PRKRA is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02744-2-prkra-primary-antibodies-ihc-testing-4.jpgAnti-PRKRA Antibody Picoband®IHC analysis of PACT/PRKRA using anti-PACT/PRKRA antibody (A02744-2). PACT/PRKRA was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PACT/PRKRA Antibody (A02744-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02744-2-prkra-primary-antibodies-ihc-testing-2.jpgAnti-PRKRA Antibody Picoband® IHC analysis of PRKRA using anti-PRKRA antibody (A02744-2). PRKRA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKRA Antibody (A02744-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02744-2-prkra-primary-antibodies-ihc-testing-3.jpgAnti-PRKRA Antibody Picoband® IHC analysis of PRKRA using anti-PRKRA antibody (A02744-2). PRKRA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKRA Antibody (A02744-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02744-2-prkra-primary-antibodies-fcm-testing-4.pngAnti-PRKRA Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-PRKRA antibody (A02744-2). Overlay histogram showing K562 cells stained with A02744-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRKRA Antibody (A02744-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-prmt3-picoband-trade-antibody-a05694-1-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05694-1-prmt3-primary-antibodies-wb-testing-1.jpgAnti-PRMT3 Antibody Picoband® Western blot analysis of PRMT3 using anti-PRMT3 antibody (A05694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT3 antigen affinity purified polyclonal antibody (Catalog # A05694-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT3 at approximately 60-70 kDa. The expected band size for PRMT3 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05694-1-prmt3-primary-antibodies-if-testing-2.jpgAnti-PRMT3 Antibody Picoband® IF analysis of PRMT3 using anti-PRMT3 antibody (A05694-1). PRMT3 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRMT3 Antibody (A05694-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05694-1-prmt3-primary-antibodies-fcm-testing-3.pngAnti-PRMT3 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-PRMT3 antibody (A05694-1). Overlay histogram showing K562 cells stained with A05694-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT3 Antibody (A05694-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rad50-picoband-trade-antibody-a00347-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00347-2-rad50-primary-antibodies-wb-testing-1_1.jpgAnti-RAD50 Antibody Picoband®Western blot analysis of RAD50 using anti-RAD50 antibody (A00347-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD50 antigen affinity purified polyclonal antibody (A00347-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAD50 at approximately 154 kDa. The expected band size for RAD50 is at 154 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00347-2-rad50-primary-antibodies-ihc-testing-1.jpgAnti-RAD50 Antibody Picoband®IHC analysis of HRAD50 using anti-RAD50 antibody (A00347-2). RAD50 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD50 Antibody (A00347-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00347-2-rad50-primary-antibodies-ihc-testing-2.jpgAnti-RAD50 Antibody Picoband®IHC analysis of HRAD50 using anti-RAD50 antibody (A00347-2). RAD50 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD50 Antibody (A00347-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00347-2-rad50-primary-antibodies-if-testing-1.jpgAnti-RAD50 Antibody Picoband®IF analysis of RAD50 using anti-RAD50 antibody (A00347-2) and anti-Tubulin Alpha antibody (M03989-3). RAD50 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAD50 Antibody (A00347-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00347-2-rad50-primary-antibodies-fcm-testing-1.jpgAnti-RAD50 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-RAD50 antibody (A00347-2). Overlay histogram showing MCF-7 cells stained with A00347-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD50 Antibody (A00347-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ror-beta-rorb-picoband-trade-antibody-a09711-3-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09711-3-rorb-primary-antibodies-wb-testing-1.jpgAnti-ROR beta/RORB Antibody Picoband® Western blot analysis of ROR Beta/RORB using anti-ROR Beta/RORB antibody (A09711-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR Beta/RORB antigen affinity purified polyclonal antibody (Catalog # A09711-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR Beta/RORB at approximately 53 kDa. The expected band size for ROR Beta/RORB is at 53 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09711-3-rorb-primary-antibodies-fcm-testing-2.pngAnti-ROR beta/RORB Antibody Picoband® Flow Cytometry analysis of Neuro-2a cells using anti-ROR Beta/RORB antibody (A09711-3). Overlay histogram showing Neuro-2a cells stained with A09711-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROR Beta/RORB Antibody (A09711-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09711-3-rorb-primary-antibodies-fcm-testing-3.pngAnti-ROR beta/RORB Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-ROR Beta/RORB antibody (A09711-3). Overlay histogram showing RH35 cells stained with A09711-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROR Beta/RORB Antibody (A09711-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-solo-sestd1-picoband-trade-antibody-a10698-1-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10698-1-sestd1-primary-antibodies-wb-testing-1.jpgAnti-Solo/SESTD1 Antibody Picoband® Western blot analysis of Solo/SESTD1 using anti-Solo/SESTD1 antibody (A10698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Solo/SESTD1 antigen affinity purified polyclonal antibody (Catalog # A10698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Solo/SESTD1 at approximately 79 kDa. The expected band size for Solo/SESTD1 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10698-1-sestd1-primary-antibodies-fcm-testing-2.pngAnti-Solo/SESTD1 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-Solo/SESTD1 antibody (A10698-1). Overlay histogram showing Daudi cells stained with A10698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Solo/SESTD1 Antibody (A10698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-taf1-picoband-trade-antibody-a02151-3-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02151-3-taf1-primary-antibodies-wb-testing-1.jpgAnti-TAF1 Antibody Picoband® Western blot analysis of TAF1 using anti-TAF1 antibody (A02151-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat bain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF1 antigen affinity purified polyclonal antibody (Catalog # A02151-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF1 at approximately 250 kDa. The expected band size for TAF1 is at 250 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02151-3-taf1-primary-antibodies-fcm-testing-2.pngAnti-TAF1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TAF1 antibody (A02151-3). Overlay histogram showing THP-1 cells stained with A02151-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAF1 Antibody (A02151-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tox-picoband-trade-antibody-a08441-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08441-2-tox-primary-antibodies-wb-testing-1.jpgAnti-TOX Antibody Picoband® Western blot analysis of TOX using anti-TOX antibody (A08441-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX antigen affinity purified polyclonal antibody (Catalog # A08441-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOX at approximately 57 kDa. The expected band size for TOX is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08441-2-tox-primary-antibodies-ihc-testing-2.jpgAnti-TOX Antibody Picoband® IHC analysis of TOX using anti-TOX antibody (A08441-2). TOX was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOX Antibody (A08441-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08441-2-tox-primary-antibodies-ihc-testing-3.jpgAnti-TOX Antibody Picoband® IHC analysis of TOX using anti-TOX antibody (A08441-2). TOX was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOX Antibody (A08441-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08441-2-tox-primary-antibodies-fcm-testing-4.pngAnti-TOX Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-TOX antibody (A08441-2). Overlay histogram showing HL-60 cells stained with A08441-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOX Antibody (A08441-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ttpa-tpp1-picoband-trade-antibody-a00103-1-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00103-1-ttpa-primary-antibodies-wb-testing-1.jpgAnti-TTPA/TPP1 Antibody Picoband® Western blot analysis of TTPA/TPP1 using anti-TTPA/TPP1 antibody (A00103-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTPA/TPP1 antigen affinity purified polyclonal antibody (Catalog # A00103-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTPA/TPP1 at approximately 32,37 kDa. The expected band size for TTPA/TPP1 is at 32 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00103-1-ttpa-primary-antibodies-fcm-testing-2.pngAnti-TTPA/TPP1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TTPA/TPP1 antibody (A00103-1). Overlay histogram showing MCF-7 cells stained with A00103-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTPA/TPP1 Antibody (A00103-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ugt1a10-picoband-trade-antibody-a04459-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04459-ugt1a10-primary-antibodies-wb-testing-1.jpgAnti-UGT1A10 Antibody Picoband® Western blot analysis of UGT1A10 using anti-UGT1A10 antibody (A04459). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A10 antigen affinity purified polyclonal antibody (Catalog # A04459) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A10 at approximately 60 kDa. The expected band size for UGT1A10 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04459-ugt1a10-primary-antibodies-fcm-testing-2.pngAnti-UGT1A10 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-UGT1A10 antibody (A04459). Overlay histogram showing Daudi cells stained with A04459(Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UGT1A10 Antibody (A04459, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/picokine-elisa-kits/human-clec5a-picokine-elisa-kit-ek2175-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2175.pngHuman CLEC5A ELISA Kit PicoKine®Human CLEC5A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-a-picokine-elisa-kit-ek2176-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2176.jpgHuman Cathepsin A ELISA Kit PicoKine®Human Cathepsin A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-c-picokine-elisa-kit-ek2177-boster.html2026-04-06T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2177.pngHuman Cathepsin C ELISA Kit PicoKine®Human Cathepsin C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-z-picokine-elisa-kit-ek2178-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2178.jpgHuman Cathepsin Z ELISA Kit PicoKine®Human Cathepsin Z PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-colec12-picokine-elisa-kit-ek2179-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2179.pngHuman COLEC12 ELISA Kit PicoKine®Human COLEC12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cpb1-picokine-elisa-kit-ek2180-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2180.jpgHuman CPB1 ELISA Kit PicoKine®Human CPB1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cpm-picokine-elisa-kit-ek2181-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2181.pngHuman CPM ELISA Kit PicoKine®Human CPM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-creld1-picokine-elisa-kit-ek2182-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2182.jpgHuman CRELD1 ELISA Kit PicoKine®Human CRELD1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dcbld2-picokine-elisa-kit-ek2183-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2183.pngHuman DCBLD2 ELISA Kit PicoKine®Human DCBLD2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-rat-dcc-picokine-elisa-kit-ek2184-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2184.jpgMouse/Rat DCC ELISA Kit PicoKine®Mouse/Rat DCC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ddah1-picokine-elisa-kit-ek2185-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2185.pngHuman DDAH1 ELISA Kit PicoKine®Mouse/Rat DCC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dpp7-picokine-elisa-kit-ek2186-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2186.pngHuman DPP7 ELISA Kit PicoKine®Human DPP7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dsc2-picokine-elisa-kit-ek2187-boster.html2026-03-10T04:33:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2187.pngHuman DSC2 ELISA Kit PicoKine®Human DSC2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pnmt-rabbit-monoclonal-antibody-m02813-2-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02813-2-wb.jpgAnti-PNMT Rabbit Monoclonal AntibodyWestern blot analysis of PNMT expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-acc-s80-rabbit-monoclonal-antibody-p01802-1-boster.html2026-03-17T05:13:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p01802-1-wb_1.jpgAnti-Phospho-ACC(S80) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-ACC(S80) expression in (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-histone-h3-3-s31-rabbit-monoclonal-antibody-p06819-5-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p06819-5-wb.jpgAnti-Phospho-Histone H3.3 (S31) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-Histone H3.3 (S31) expression in HeLa cell treated with Calyculin A lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il-33-rabbit-monoclonal-antibody-m00113-2-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00113-2-wb.jpgAnti-IL-33 Rabbit Monoclonal AntibodyWestern blot analysis of IL-33 expression in mouse lung cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bag1-rabbit-monoclonal-antibody-m02423-2-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02423-2-wb.jpgAnti-Bag1 Rabbit Monoclonal AntibodyWestern blot analysis of Bag1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p2y12-rabbit-monoclonal-antibody-m01136-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01136-p2ry12-primary-antibodies-wb-testing-1.jpgAnti-P2Y12 Rabbit Monoclonal Antibody Western blot analysis of P2RY12 using anti-P2RY12 antibody (M01136). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U-87 MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RY12 antigen affinity purified monoclonal antibody (Catalog # M01136) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RY12 at approximately 50 kDa. The expected band size for P2RY12 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01136-ip7.jpgAnti-P2Y12 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-nucleophosmin-t199-rabbit-monoclonal-antibody-p00450-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00450-wb.jpgAnti-Phospho-Nucleophosmin (T199) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-Nucleophosmin (T199) expression in (1) HeLa cell lysate; (2) HeLa cell treated with CA lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hnf-4-alpha-rabbit-monoclonal-antibody-m00389-3-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-3-hnf4a-primary-antibodies-wb-testing-1.jpgAnti-HNF-4-alpha Rabbit Monoclonal AntibodyWestern blot analysis of HNF4A using anti-HNF4A antibody (M00389-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF4A antigen affinity purified monoclonal antibody (M00389-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HNF4A at approximately 53 kDa. The expected band size for HNF4A is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-3-hnf4a-primary-antibodies-ihc-testing-1.jpgAnti-HNF-4-alpha Rabbit Monoclonal AntibodyIHC analysis of HNF4A using anti-HNF4A antibody (M00389-3). HNF4A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNF4A Antibody (M00389-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-3-hnf4a-primary-antibodies-ihc-testing-2.jpgAnti-HNF-4-alpha Rabbit Monoclonal AntibodyIHC analysis of HNF4A using anti-HNF4A antibody (M00389-3). HNF4A was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNF4A Antibody (M00389-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-3-hnf4a-primary-antibodies-ihc-testing-3.jpgAnti-HNF-4-alpha Rabbit Monoclonal AntibodyIHC analysis of HNF4A using anti-HNF4A antibody (M00389-3). HNF4A was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNF4A Antibody (M00389-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-3-hnf4a-primary-antibodies-ihc-testing-4.jpgAnti-HNF-4-alpha Rabbit Monoclonal AntibodyIHC analysis of HNF4A using anti-HNF4A antibody (M00389-3). HNF4A was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNF4A Antibody (M00389-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hnf-4-alpha-rabbit-monoclonal-antibody-m00389-4-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00389-4-hnf-4-alpha-primary-antibodies-wb-testing-1.jpgAnti-HNF-4-alpha Rabbit Monoclonal Antibody Western blot analysis of HNF-4-Alpha using anti-HNF-4-Alpha antibody (M00389-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF-4-Alpha antigen affinity purified monoclonal antibody (M00389-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF-4-Alpha at approximately 53 kDa. The expected band size for HNF-4-Alpha is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lactate-dehydrogenase-c-rabbit-monoclonal-antibody-m08060-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08060-wb.jpgAnti-Lactate Dehydrogenase C Rabbit Monoclonal AntibodyWestern blot analysis of Lactate Dehydrogenase C expression in human testis lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hif-prolyl-hydroxylases-rabbit-monoclonal-antibody-m12260-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12260-wb.jpgAnti-HIF Prolyl Hydroxylases Rabbit Monoclonal AntibodyWestern blot analysis of HIF Prolyl Hydroxylases expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12260-ip7.jpgAnti-HIF Prolyl Hydroxylases Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crtc3-rabbit-monoclonal-antibody-m04568-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04568-wb.jpgAnti-CRTC3 Rabbit Monoclonal AntibodyWestern blot analysis of CRTC3 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rsk2-rps6ka3-rabbit-monoclonal-antibody-m02215-1-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02215-1-rsk2-primary-antibodies-wb-testing-1.jpgAnti-RSK2 / RPS6KA3 Rabbit Monoclonal AntibodyWestern blot analysis of RSK2 using anti-RSK2 antibody (M02215-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSK2 antigen affinity purified monoclonal antibody (M02215-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RSK2 at approximately 84 kDa. The expected band size for RSK2 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02215-1-rps6ka3-primary-antibodies-ihc-testing-1.jpgAnti-RSK2 / RPS6KA3 Rabbit Monoclonal AntibodyIHC analysis of RSK2/RPS6KA3 using anti-RSK2/RPS6KA3 antibody (M02215-1). RSK2/RPS6KA3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RSK2/RPS6KA3 Antibody (M02215-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02215-1-rps6ka3-primary-antibodies-ihc-testing-2.jpgAnti-RSK2 / RPS6KA3 Rabbit Monoclonal AntibodyIHC analysis of RSK2/RPS6KA3 using anti-RSK2/RPS6KA3 antibody (M02215-1). RSK2/RPS6KA3 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RSK2/RPS6KA3 Antibody (M02215-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02215-1-rps6ka3-primary-antibodies-ihc-testing-3.jpgAnti-RSK2 / RPS6KA3 Rabbit Monoclonal AntibodyIHC analysis of RSK2/RPS6KA3 using anti-RSK2/RPS6KA3 antibody (M02215-1). RSK2/RPS6KA3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RSK2/RPS6KA3 Antibody (M02215-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rad51d-rabbit-monoclonal-antibody-m02893-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02893-wb.jpgAnti-Rad51D Rabbit Monoclonal AntibodyWestern blot analysis of Rad51D expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-semaphorin-4d-cd100-rabbit-monoclonal-antibody-m02512-1-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02512-1-wb.jpgAnti-Semaphorin 4D / CD100 Rabbit Monoclonal AntibodyWestern blot analysis of Semaphorin 4D / CD100 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-icam2-cd102-rabbit-monoclonal-antibody-m04901-1-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04901-1-wb.jpgAnti-ICAM2 / CD102 Rabbit Monoclonal AntibodyWestern blot analysis of ICAM2 / CD102 expression in mouse heart cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04901-1-ip7.jpgAnti-ICAM2 / CD102 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thrombomodulin-cd141-rabbit-monoclonal-antibody-m01325-2-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01325-2-wb.jpgAnti-Thrombomodulin / CD141 Rabbit Monoclonal AntibodyWestern blot analysis of Thrombomodulin / CD141 expression in mouse lung cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-cbl-y774-rabbit-monoclonal-antibody-p00152-boster.html2026-03-24T05:36:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00152-cbl-primary-antibodies-wb-testing-1.jpgAnti-Phospho-CBL (Y774) Rabbit Monoclonal AntibodyWestern blot analysis of c-Cbl/CBL using anti-c-Cbl/CBL antibody (P00152). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Cbl/CBL antigen affinity purified monoclonal antibody (P00152) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for c-Cbl/CBL at approximately 120 kDa. The expected band size for c-Cbl/CBL is at 100 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-rad17-s656-rabbit-monoclonal-antibody-p02159-1-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02159-1-wb.jpgAnti-Phospho-Rad17 (S656) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-Rad17 (S656) expression in (1) HeLa cell treated with CA lysate; (2) HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eya2-rabbit-monoclonal-antibody-m05074-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05074-wb.jpgAnti-EYA2 Rabbit Monoclonal AntibodyWestern blot analysis of EYA2 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-galectin-3-rabbit-monoclonal-antibody-m00621-4-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00621-4-lgals3-primary-antibodies-wb-testing-1.jpgAnti-Galectin 3 Rabbit Monoclonal Antibody Western blot analysis of Galectin 3 using anti-Galectin 3 antibody (M00621-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3 antigen affinity purified monoclonal antibody (Catalog # M00621-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 3 at approximately 29 kDa. The expected band size for Galectin 3 is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd44-rabbit-monoclonal-antibody-m00052-4-boster.html2026-03-17T05:13:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00052-4-cd44-primary-antibodies-wb-testing-1.jpgAnti-CD44 Rabbit Monoclonal Antibody Western blot analysis of CD44 using anti-CD44 antibody (M00052-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified monoclonal antibody (Catalog # M00052-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 82 kDa. The expected band size for CD44 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00052-4-ip7.jpgAnti-CD44 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-galectin-1-rabbit-monoclonal-antibody-m00470-3-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00470-3-wb.jpgAnti-Galectin 1 Rabbit Monoclonal AntibodyWestern blot analysis of Galectin 1 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gclm-rabbit-monoclonal-antibody-m02948-2-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-wb-testing-1.jpgAnti-GCLM Rabbit Monoclonal Antibody Western blot analysis of GCLM using anti-GCLM antibody (M02948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified monoclonal antibody (Catalog # M02948-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 28 kDa. The expected band size for GCLM is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-ip7.jpgAnti-GCLM Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-2.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-3.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-4.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-5.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-6.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-7.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-8.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-9.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-10.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-11.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-12.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-13.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-14.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-15.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-16.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of mouse adrenal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-17.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of mouse adrenal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-18.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-19.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-20.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-2-gclm-primary-antibodies-ihc-testing-21.jpgAnti-GCLM Rabbit Monoclonal Antibody IHC analysis of GCLM using anti-GCLM antibody (M02948-2). GCLM was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GCLM Antibody (M02948-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adiponectin-rabbit-monoclonal-antibody-m00509-2-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-2-wb.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyWestern blot analysis of Adiponectin expression in human plasma cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-19-rabbit-monoclonal-antibody-m02101-7-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-7-krt19-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyFigure 1. Western blot analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 19/KRT19 antigen affinity purified monoclonal antibody (M02101-7) at a dilution of 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cytokeratin 19/KRT19 at approximately 40 kDa. The expected band size for Cytokeratin 19/KRT19 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-7-krt19-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyFigure 2. IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-7). Cytokeratin 19/KRT19 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Cytokeratin 19/KRT19 Antibody (M02101-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-7-krt19-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyFigure 3. IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-7). Cytokeratin 19/KRT19 was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Cytokeratin 19/KRT19 Antibody (M02101-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-7-krt19-primary-antibodies-ihc-testing-4.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyFigure 4. IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-7). Cytokeratin 19/KRT19 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Cytokeratin 19/KRT19 Antibody (M02101-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clpx-rabbit-monoclonal-antibody-m00978-1-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00978-1-wb.jpgAnti-CLPX Rabbit Monoclonal AntibodyWestern blot analysis of CLPX expression in (1) A673 cell lysate; (2) Mouse brain lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-epsin-1-rabbit-monoclonal-antibody-m01870-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01870-wb.jpgAnti-Epsin 1 Rabbit Monoclonal AntibodyWestern blot analysis of Epsin 1 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gfpt1-rabbit-monoclonal-antibody-m04341-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04341-wb.jpgAnti-GFPT1 Rabbit Monoclonal AntibodyWestern blot analysis of GFPT1 expression in MCF7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04341-wb7.jpgAnti-GFPT1 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-map3k4-rabbit-monoclonal-antibody-m04842-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04842-map3k4-primary-antibodies-wb-testing-1.jpgAnti-MAP3K4 Rabbit Monoclonal AntibodyWestern blot analysis of MAP3K4 using anti-MAP3K4 antibody (M04842). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K4 antigen affinity purified monoclonal antibody (M04842) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP3K4 at approximately 182 kDa. The expected band size for MAP3K4 is at 182 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ufm1-rabbit-monoclonal-antibody-m05543-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05543-wb.jpgAnti-UFM1 Rabbit Monoclonal AntibodyWestern blot analysis of UFM1 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05543-ufm1-primary-antibodies-ihc-testing-1.jpgAnti-UFM1 Rabbit Monoclonal AntibodyIHC analysis of UFM1 using anti-UFM1 antibody (M05543). UFM1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-UFM1 Antibody (M05543) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05543-ufm1-primary-antibodies-ihc-testing-2.jpgAnti-UFM1 Rabbit Monoclonal AntibodyIHC analysis of UFM1 using anti-UFM1 antibody (M05543). UFM1 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-UFM1 Antibody (M05543) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05543-ip7.jpgAnti-UFM1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uba52-rabbit-monoclonal-antibody-m04905-1-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04905-uba52-primary-antibodies-wb-testing-1.jpgAnti-UBA52 Rabbit Monoclonal AntibodyWestern blot analysis of UBA52 using anti-UBA52 antibody (M01970). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA52 antigen affinity purified monoclonal antibody (M01970) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBA52 at approximately 15 kDa. The expected band size for UBA52 is at 10 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mef2a-rabbit-monoclonal-antibody-m01398-1-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01398-1-wb.jpgAnti-MEF2A Rabbit Monoclonal AntibodyWestern blot analysis of MEF2A expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p60-katanin-rabbit-monoclonal-antibody-m09752-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09752-wb.jpgAnti-p60 katanin Rabbit Monoclonal AntibodyWestern blot analysis of p60 katanin expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-cbl-s669-rabbit-monoclonal-antibody-p00152-1-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00152-1-wb.jpgAnti-Phospho-CBL (S669) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-CBL (S669) expression in (1) HeLa cell lysate; (2) HeLa cell treated with pervanadate lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grk6-rabbit-monoclonal-antibody-m03623-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03623-wb.jpgAnti-GRK6 Rabbit Monoclonal AntibodyWestern blot analysis of GRK6 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-icam3-cd50-rabbit-monoclonal-antibody-m03651-4-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03651-4-wb.jpgAnti-ICAM3 / CD50 Rabbit Monoclonal AntibodyWestern blot analysis of ICAM3 / CD50 expression in Ramos cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyclin-h-p34-rabbit-monoclonal-antibody-m03013-3-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03013-3-wb.jpgAnti-Cyclin H/p34 Rabbit Monoclonal AntibodyWestern blot analysis of Cyclin H/p34 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufb9-rabbit-monoclonal-antibody-m08623-3-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08623-3-wb.jpgAnti-NDUFB9 Rabbit Monoclonal AntibodyWestern blot analysis of NDUFB9 expression in HEK293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grsf1-rabbit-monoclonal-antibody-m07576-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07576-wb.jpgAnti-GRSF1 Rabbit Monoclonal AntibodyWestern blot analysis of GRSF1 expression in 293 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07576-ip7.jpgAnti-GRSF1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zhx2-rabbit-monoclonal-antibody-m05837-1-boster.html2026-03-17T05:13:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05837-1-zhx2-primary-antibodies-wb-testing-1_1.jpgAnti-ZHX2 Rabbit Monoclonal AntibodyWestern blot analysis of ZHX2 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05837-1-zhx2-primary-antibodies-wb-testing-2.jpgAnti-ZHX2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-endogl1-engl-rabbit-monoclonal-antibody-m10155-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10155-wb.jpgAnti-ENDOGL1 / ENGL Rabbit Monoclonal AntibodyWestern blot analysis of ENDOGL1 / ENGL expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10155-ip7.jpgAnti-ENDOGL1 / ENGL Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plcb1-rabbit-monoclonal-antibody-m02817-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02817-plcb1-primary-antibodies-wb-testing-1.jpgAnti-PLCB1 Rabbit Monoclonal AntibodyWestern blot analysis of PLCB1 using anti-PLCB1 antibody (M02817). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCB1 antigen affinity purified monoclonal antibody (M02817) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLCB1 at approximately 150 kDa. The expected band size for PLCB1 is at 139 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab5c-rabl-rabbit-monoclonal-antibody-m05148-1-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05148-1-rab5c-primary-antibodies-wb-testing-1_1.jpgAnti-RAB5C / RABL Rabbit Monoclonal Antibody Western blot analysis of RAB5C/RABL using anti-RAB5C/RABL antibody (M05148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5C/RABL antigen affinity purified monoclonal antibody (Catalog # M05148-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5C/RABL at approximately 23 kDa. The expected band size for RAB5C/RABL is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slp2-rabbit-monoclonal-antibody-m04108-1-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04108-1-stoml2-primary-antibodies-wb-testing-1.jpgAnti-SLP2 Rabbit Monoclonal Antibody Western blot analysis of STOML2 using anti-STOML2 antibody (M04108-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STOML2 antigen affinity purified monoclonal antibody (M04108-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STOML2 at approximately 39 kDa. The expected band size for STOML2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04108-1-ip7.jpgAnti-SLP2 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-arfrp1-arp-rabbit-monoclonal-antibody-m08327-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08327-wb.jpgAnti-ARFRP1 / ARP Rabbit Monoclonal AntibodyWestern blot analysis of ARFRP1 / ARP expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fdps-rabbit-monoclonal-antibody-m01782-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01782-wb.jpgAnti-FDPS Rabbit Monoclonal AntibodyWestern blot analysis of FDPS expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cystatin-b-rabbit-monoclonal-antibody-m02794-1-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02794-1-wb.jpgAnti-Cystatin B Rabbit Monoclonal AntibodyWestern blot analysis of Cystatin B expression in U87-MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcm2-rabbit-monoclonal-antibody-m00374-2-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00374-2-wb.jpgAnti-MCM2 Rabbit Monoclonal AntibodyWestern blot analysis of MCM2 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00374-2-ip7.jpgAnti-MCM2 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nck2-rabbit-monoclonal-antibody-m04036-1-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04036-1-nck2-primary-antibodies-wb-testing-1.jpgAnti-Nck2 Rabbit Monoclonal Antibody Western blot analysis of NCK2 using anti-NCK2 antibody (M04036-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCK2 antigen affinity purified monoclonal antibody (Catalog # M04036-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCK2 at approximately 43 kDa. The expected band size for NCK2 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ube2l3-ubch7-rabbit-monoclonal-antibody-m01832-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01832-wb.jpgAnti-Ube2L3 / UBCH7 Rabbit Monoclonal AntibodyWestern blot analysis of Ube2L3 / UBCH7 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldh7a1-rabbit-monoclonal-antibody-m03712-1-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03712-1-wb.jpgAnti-ALDH7A1 Rabbit Monoclonal AntibodyWestern blot analysis of ALDH7A1 expression in SW480 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmg4-rabbit-monoclonal-antibody-m02834-2-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02834-2-wb.jpgAnti-HMG4 Rabbit Monoclonal AntibodyWestern blot analysis of HMG4 expression in Hela cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-mcm2-s27-rabbit-monoclonal-antibody-p00374-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00374-wb.jpgAnti-Phospho-MCM2 (S27) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-MCM2 (S27) expression in (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-amsh-rabbit-monoclonal-antibody-m05964-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05964-wb.jpgAnti-AMSH Rabbit Monoclonal AntibodyWestern blot analysis of AMSH expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-pts-s19-rabbit-monoclonal-antibody-p00430-boster.html2026-03-17T05:13:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00430-wb.jpgAnti-Phospho-PTS (S19) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-PTS (S19) expression in (1) HUVEC cell lysate; (2) HUVEC cell treated with Alkaline Phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-frat1-rabbit-monoclonal-antibody-m08049-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08049-wb.jpgAnti-FRAT1 Rabbit Monoclonal AntibodyWestern blot analysis of FRAT1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trappc2-rabbit-monoclonal-antibody-m04968-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04968-wb.jpgAnti-TRAPPC2 Rabbit Monoclonal AntibodyWestern blot analysis of TRAPPC2 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cip4-rabbit-monoclonal-antibody-m03963-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03963-wb.jpgAnti-Cip4 Rabbit Monoclonal AntibodyWestern blot analysis of Cip4 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-mcm2-s41-rabbit-monoclonal-antibody-p00374-1-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00374-1-wb.jpgAnti-Phospho-MCM2 (S41) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-MCM2 (S41) expression in (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lat-rabbit-monoclonal-antibody-m01654-1-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01654-1-wb.jpgAnti-LAT Rabbit Monoclonal AntibodyWestern blot analysis of LAT expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-r-cadherin-rabbit-monoclonal-antibody-m07632-1-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07632-1-cdh4-primary-antibodies-wb-testing-1.jpgAnti-R Cadherin Rabbit Monoclonal AntibodyWestern blot analysis of CDH4 using anti-CDH4 antibody (M07632-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human U87-MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH4 antigen affinity purified monoclonal antibody (M07632-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDH4 at approximately 130 kDa. The expected band size for CDH4 is at 100 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-blnk-rabbit-monoclonal-antibody-m03630-2-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03630-2-wb.jpgAnti-BLNK Rabbit Monoclonal AntibodyWestern blot analysis of BLNK expression in Raji cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03630-2-ip7.jpgAnti-BLNK Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyb5r3-rabbit-monoclonal-antibody-m03487-2-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03487-2-wb.jpgAnti-CYB5R3 Rabbit Monoclonal AntibodyWestern blot analysis of CYB5R3 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-mcm2-s108-rabbit-monoclonal-antibody-p00374-2-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00374-2-wb.jpgAnti-Phospho-MCM2 (S108) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-MCM2 (S108) expression in (1) HeLa cell lysate; (2) HeLa cell treated with alkaline phosphatase lysate. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00374-2-ip7.jpgAnti-Phospho-MCM2 (S108) Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-torc2-rabbit-monoclonal-antibody-m01118-1-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01118-1-wb.jpgAnti-TORC2 Rabbit Monoclonal AntibodyWestern blot analysis of TORC2 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nodal-rabbit-monoclonal-antibody-m07627-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07627-wb.jpgAnti-Nodal Rabbit Monoclonal AntibodyWestern blot analysis of Nodal expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hint1-rabbit-monoclonal-antibody-m02557-1-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02557-1-hint1-primary-antibodies-wb-testing-1.jpgAnti-HINT1 Rabbit Monoclonal AntibodyWestern blot analysis of HINT1 using anti-HINT1 antibody (M02557-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HINT1 antigen affinity purified monoclonal antibody (M02557-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HINT1 at approximately 14 kDa. The expected band size for HINT1 is at 14 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adx-rabbit-monoclonal-antibody-m05441-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fgene-14-1071694-g001.jpgAnti-ADX Rabbit Monoclonal AntibodyFDX1, DLAT and CDKN2A cuproptosis-related genes prognostic model. (A) 8,962 potential prognostic molecules of KIRC and 10 cuproptosis-related genes Venn diagram. (B) LASSO variable trajectory diagram. (C) LASSO coefficient screening diagram. (D) The prognostic risk factor graph, red represents high-risk group, blue represents low-risk group. (E) Kaplan-Meier survival curve and time dependent ROC. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fgene.2023.1071694/full'>36755576</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fgene-14-1071694-g003.jpgAnti-ADX Rabbit Monoclonal AntibodyThe expression difference of FDX1 in KIRC. (A) Differential expression of FDX1 in paired samples in TCGA-KIRC database. (B) Differential expression of FDX1 in unpaired samples in TCGA-KIRC database. (C) Differential expression of FDX1 in GSE36895. (D) Differential expression of FDX1 in GSE53757. (E) Differential expression of FDX1 between KIRC patients and normal renal tissue by RT-qPCR. (F) Differential expression of FDX1 in TCGA pan cancer. NS, p > .05; *, p < .05; **, p < .01; ***, p < .001. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fgene.2023.1071694/full'>36755576</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fgene-14-1071694-g004.jpgAnti-ADX Rabbit Monoclonal AntibodyRepresentative images of FDX1 expression in KIRC tissues and their matched paracancerous tissues. Original magnifications ×100 and 400× (inset panels). Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fgene.2023.1071694/full'>36755576</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fgene-14-1071694-g006.jpgAnti-ADX Rabbit Monoclonal AntibodyCorrelation between FDX1 and other nine cuproptosis genes. (A) FDX1 and other nine cuproptosis-related molecules co-expression heatmap. (B) 10 cuproptosis-related genes correlation heatmap. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fgene.2023.1071694/full'>36755576</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fgene-14-1071694-g005.jpgAnti-ADX Rabbit Monoclonal AntibodyThe correlation between FDX1 and clinical characteristics in KIRC. (A) The correlation between FDX1 and T stage. (B) The correlation between FDX1 and N stage. (C) The correlation between FDX1 and M stage. (D) The correlation between FDX1 and Age. (E) The correlation between FDX1 and Gender. (F) The correlation between FDX1 and Histologic grade. (G) The correlation between FDX1 and Pathologic stage. (H) The correlation between FDX1 and Laterality. NS, p > .05; *, p < .05; **, p < .01; ***, p < .001. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fgene.2023.1071694/full'>36755576</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fgene-14-1071694-g007.jpgAnti-ADX Rabbit Monoclonal AntibodyThe expression and the prognosis analysis of immune checkpoints. (A) Expression of immune checkpoint in high and low expression groups of FDX1. (B) Scatter plot of immune checkpoint association with FDX1. (C) Overall survival curve of immune checkpoints in KIRC patients. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fgene.2023.1071694/full'>36755576</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fgene-14-1071694-g008.jpgAnti-ADX Rabbit Monoclonal AntibodyRelationship among FDX1 expression, ICB and immune cell infiltration in KIRC. (A) Distribution of immune response scores in high and low expression groups of FDX1. (B) The abundance of immune cell infiltration in FDX1-low group and FDX1-high group in TCGA-KIRC. (C) The Kaplan-Meier curve of Endothelial cells in KIRC. G1 group represented FDX1-high group, G2 group represented FDX1-low group. (D) The abundance of immune cell infiltration in FDX1-low group and FDX1-high group in GSE53757. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fgene.2023.1071694/full'>36755576</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-fdx1-primary-antibodies-wb-testing-1_1.jpgAnti-ADX Rabbit Monoclonal Antibody Western blot analysis of FDX1 using anti-FDX1 antibody (M05441). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FDX1 antigen affinity purified monoclonal antibody (Catalog # M05441) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FDX1 at approximately 13 kDa. The expected band size for FDX1 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05441-icc1.jpgAnti-ADX Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdlbp-rabbit-monoclonal-antibody-m06727-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06727-wb.jpgAnti-HDLBP Rabbit Monoclonal AntibodyWestern blot analysis of HDLBP expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-taok1-2-3-s181-s181-s177-rabbit-monoclonal-antibody-p07063-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p07063-wb.jpgAnti-Phospho-TAOK1/2/3 (S181 + S181 + S177) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-TAOK1/2/3 (S181 + S181 + S177) expression in (1) 293 cell lysate; (2) 293 cell treated with Lambda Phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-csn8-rabbit-monoclonal-antibody-m07142-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07142-wb.jpgAnti-CSN8 Rabbit Monoclonal AntibodyWestern blot analysis of CSN8 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nat1-rabbit-monoclonal-antibody-m01386-1-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01386-1-wb.jpg.jpgAnti-NAT1 Rabbit Monoclonal AntibodyWestern blot analysis of NAT1 expression in LnCaP cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cadherin-10-rabbit-monoclonal-antibody-m10616-boster.html2026-03-17T05:13:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10616-wb.jpgAnti-Cadherin 10 Rabbit Monoclonal AntibodyWestern blot analysis of Cadherin 10 expression in U-87 MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-denn-rabbit-monoclonal-antibody-m02030-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02030-wb.jpgAnti-DENN Rabbit Monoclonal AntibodyWestern blot analysis of DENN expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mpp1-rabbit-monoclonal-antibody-m05793-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05793-wb.jpgAnti-MPP1 Rabbit Monoclonal AntibodyWestern blot analysis of MPP1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wnt16-rabbit-monoclonal-antibody-m03606-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03606-wb.jpgAnti-Wnt16 Rabbit Monoclonal AntibodyWestern blot analysis of Wnt16 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sara-rabbit-monoclonal-antibody-m07533-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07533-wb.jpgAnti-SARA Rabbit Monoclonal AntibodyWestern blot analysis of SARA expression in A673 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-importin-9-ranbp9-rabbit-monoclonal-antibody-m10216-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10216-wb.jpgAnti-Importin 9 / RANBP9 Rabbit Monoclonal AntibodyWestern blot analysis of Importin 9 / RANBP9 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10216-wb7.jpgAnti-Importin 9 / RANBP9 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uba3-rabbit-monoclonal-antibody-m05116-2-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05116-2-wb.jpgAnti-UBA3 Rabbit Monoclonal AntibodyWestern blot analysis of UBA3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usf1-rabbit-monoclonal-antibody-m02500-1-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02500-1-wb.jpgAnti-USF1 Rabbit Monoclonal AntibodyWestern blot analysis of USF1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ing1-rabbit-monoclonal-antibody-m02999-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02999-wb.jpgAnti-ING1 Rabbit Monoclonal AntibodyWestern blot analysis of ING1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rassf2-rabbit-monoclonal-antibody-m04384-1-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04384-1-wb.jpgAnti-RASSF2 Rabbit Monoclonal AntibodyWestern blot analysis of RASSF2 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdlbp-rabbit-monoclonal-antibody-m06727-1-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06727-1-wb.jpgAnti-HDLBP Rabbit Monoclonal AntibodyWestern blot analysis of HDLBP expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pleckstrin-rabbit-monoclonal-antibody-m06656-2-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06656-2-wb.jpgAnti-Pleckstrin Rabbit Monoclonal AntibodyWestern blot analysis of Pleckstrin expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mvk-rabbit-monoclonal-antibody-m02465-1-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02465-1-wb.jpgAnti-MVK Rabbit Monoclonal AntibodyWestern blot analysis of MVK expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mtap-rabbit-monoclonal-antibody-m05448-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05448-wb.jpgAnti-MTAP Rabbit Monoclonal AntibodyWestern blot analysis of MTAP expression in (1) 293T cell lysate; (2) NIH/3T3 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05448-ip7.jpgAnti-MTAP Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-n4bp1-rabbit-monoclonal-antibody-m12575-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12575-wb.jpgAnti-N4BP1 Rabbit Monoclonal AntibodyWestern blot analysis of N4BP1 expression in PC12 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gna13-rabbit-monoclonal-antibody-m04160-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04160-wb.jpgAnti-GNA13 Rabbit Monoclonal AntibodyWestern blot analysis of GNA13 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bckdk-rabbit-monoclonal-antibody-m07798-1-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07798-1-wb.jpgAnti-BCKDK Rabbit Monoclonal AntibodyWestern blot analysis of BCKDK expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rgs6-rabbit-monoclonal-antibody-m05753-boster.html2026-03-17T05:13:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05753-wb.jpgAnti-RGS6 Rabbit Monoclonal AntibodyWestern blot analysis of RGS6 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ltk-rabbit-monoclonal-antibody-m04217-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04217-wb.jpgAnti-LTK Rabbit Monoclonal AntibodyWestern blot analysis of LTK expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-eif4b-s406-rabbit-monoclonal-antibody-p00846-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00846-wb.jpgAnti-Phospho-eIF4B (S406) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-eIF4B (S406) expression in (1) HeLa cell lysate; (2) HeLa cell treated with Alkaline Phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nucleolin-rabbit-monoclonal-antibody-m00228-2-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00228-2-wb.jpgAnti-Nucleolin Rabbit Monoclonal AntibodyWestern blot analysis of Nucleolin expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clip170-rabbit-monoclonal-antibody-m08907-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08907-clip170-primary-antibodies-wb-testing-1.jpgAnti-CLIP170 Rabbit Monoclonal Antibody Western blot analysis of CLIP170 using anti-CLIP170 antibody (M08907). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whoel cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIP170 antigen affinity purified monoclonal antibody (M08907) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIP170 at approximately 170 kDa. The expected band size for CLIP170 is at 162 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-akap-95-rabbit-monoclonal-antibody-m05133-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05133-wb.jpgAnti-AKAP 95 Rabbit Monoclonal AntibodyWestern blot analysis of AKAP 95 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbm3-rabbit-monoclonal-antibody-m03104-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03104-rbm3-primary-antibodies-wb-testing-1_1.jpgAnti-RBM3 Rabbit Monoclonal Antibody Western blot analysis of RBM3 using anti-RBM3 antibody (M03104). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM3 antigen affinity purified monoclonal antibody (Catalog # M03104) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM3 at approximately 17 kDa. The expected band size for RBM3 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03104-rbm3-primary-antibodies-ihc-testing-2.jpgAnti-RBM3 Rabbit Monoclonal Antibody IHC analysis of RBM3 using anti-RBM3 antibody (M03104). RBM3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RBM3 Antibody (M03104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03104-rbm3-primary-antibodies-ihc-testing-3.jpgAnti-RBM3 Rabbit Monoclonal Antibody IHC analysis of RBM3 using anti-RBM3 antibody (M03104). RBM3 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RBM3 Antibody (M03104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03104-rbm3-primary-antibodies-ihc-testing-4.jpgAnti-RBM3 Rabbit Monoclonal Antibody IHC analysis of RBM3 using anti-RBM3 antibody (M03104). RBM3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RBM3 Antibody (M03104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03104-rbm3-primary-antibodies-ihc-testing-5.jpgAnti-RBM3 Rabbit Monoclonal Antibody IHC analysis of RBM3 using anti-RBM3 antibody (M03104). RBM3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RBM3 Antibody (M03104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03104-rbm3-primary-antibodies-ihc-testing-6.jpgAnti-RBM3 Rabbit Monoclonal Antibody IHC analysis of RBM3 using anti-RBM3 antibody (M03104). RBM3 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RBM3 Antibody (M03104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03104-rbm3-primary-antibodies-if-testing-7.jpgAnti-RBM3 Rabbit Monoclonal Antibody IF analysis of RBM3 using anti-RBM3 antibody (M03104) and anti-Beta Tubulin antibody (M01857-3). RBM3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-RBM3 Antibody (M03104) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctp-synthase-ctps-rabbit-monoclonal-antibody-m06374-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06374-wb.jpgAnti-CTP synthase / CTPS Rabbit Monoclonal AntibodyWestern blot analysis of CTP synthase / CTPS expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf14-rabbit-monoclonal-antibody-m08956-1-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08956-1-wb.jpgAnti-RNF14 Rabbit Monoclonal AntibodyWestern blot analysis of RNF14 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sstr3-rabbit-monoclonal-antibody-m04188-1-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04188-1-wb.jpgAnti-SSTR3 Rabbit Monoclonal AntibodyWestern blot analysis of SSTR3 expression in Somatostatin Receptor 3 SSTR3 transfected 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pef1-rabbit-monoclonal-antibody-m09591-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09591-wb.jpgAnti-PEF1 Rabbit Monoclonal AntibodyWestern blot analysis of PEF1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plscr3-rabbit-monoclonal-antibody-m10575-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10575-wb.jpgAnti-PLSCR3 Rabbit Monoclonal AntibodyWestern blot analysis of PLSCR3 expression in BxPC 3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spink5-lekti-rabbit-monoclonal-antibody-m03254-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03254-wb.jpgAnti-SPINK5 / LEKTI Rabbit Monoclonal AntibodyWestern blot analysis of SPINK5 / LEKTI expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03254-wb7.jpgAnti-SPINK5 / LEKTI Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppcs-rabbit-monoclonal-antibody-m05567-1-boster.html2026-03-17T05:13:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05567-1-wb.jpgAnti-PPCS Rabbit Monoclonal AntibodyWestern blot analysis of PPCS expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nup50-rabbit-monoclonal-antibody-m06152-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06152-nup50-primary-antibodies-wb-testing-1.jpgAnti-NUP50 Rabbit Monoclonal Antibody Western blot analysis of NUP50 using anti-NUP50 antibody (M06152). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP50 antigen affinity purified monoclonal antibody (M06152) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP50 at approximately 55 kDa. The expected band size for NUP50 is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-nucleolin-t84-rabbit-monoclonal-antibody-p00228-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00228-wb.jpgAnti-Phospho-Nucleolin (T84) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-Nucleolin (T84) expression in (1) K562 cell lysate; (2) K562 cell treated with Calyculin A lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oxgr1-gpr99-rabbit-monoclonal-antibody-m12225-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12225-wb.jpgAnti-OXGR1 / GPR99 Rabbit Monoclonal AntibodyWestern blot analysis of OXGR1 / GPR99 expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pin4-rabbit-monoclonal-antibody-m05181-1-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05181-1-pin4-primary-antibodies-wb-testing-1.jpgAnti-PIN4 Rabbit Monoclonal Antibody Western blot analysis of PIN4 using anti-PIN4 antibody (M05181-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates. Lane 5: mouse liver tissue lysates. Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIN4 antigen affinity purified monoclonal antibody (Catalog # M05181-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIN4 at approximately 14 kDa. The expected band size for PIN4 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05181-1-pin4-primary-antibodies-ihc-testing-2.jpgAnti-PIN4 Rabbit Monoclonal Antibody IHC analysis of PIN4 using anti-PIN4 antibody (M05181-1). PIN4 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIN4 Antibody (M05181-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05181-1-pin4-primary-antibodies-ihc-testing-3.jpgAnti-PIN4 Rabbit Monoclonal Antibody IHC analysis of PIN4 using anti-PIN4 antibody (M05181-1). PIN4 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PIN4 Antibody (M05181-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nup50-rabbit-monoclonal-antibody-m06152-1-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06152-1-wb.jpgAnti-NUP50 Rabbit Monoclonal AntibodyWestern blot analysis of NUP50 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06152-1-wb9.jpgAnti-NUP50 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06152-1-wb7.jpgAnti-NUP50 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06152-1-wb10.jpgAnti-NUP50 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06152-1-wb8.jpgAnti-NUP50 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ephx2-rabbit-monoclonal-antibody-m01999-3-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01999-3-wb.jpgAnti-EPHX2 Rabbit Monoclonal AntibodyWestern blot analysis of EPHX2 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dtymk-rabbit-monoclonal-antibody-m12209-1-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12209-1-dtymk-primary-antibodies-wb-testing-1.jpgAnti-DTYMK Rabbit Monoclonal Antibody Western blot analysis of DTYMK using anti-DTYMK antibody (M12209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTYMK antigen affinity purified monoclonal antibody (Catalog # M12209-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DTYMK at approximately 24 kDa. The expected band size for DTYMK is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tpmt-rabbit-monoclonal-antibody-m00671-2-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00671-2-wb.jpgAnti-TPMT Rabbit Monoclonal AntibodyWestern blot analysis of TPMT expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnajc12-rabbit-monoclonal-antibody-m13814-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13814-wb.jpgAnti-DNAJC12 Rabbit Monoclonal AntibodyWestern blot analysis of DNAJC12 expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyfip1-rabbit-monoclonal-antibody-m04596-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04596-cyfip1-primary-antibodies-wb-testing-1.jpgAnti-CYFIP1 Rabbit Monoclonal Antibody Western blot analysis of CYFIP1 using anti-CYFIP1 antibody (M04596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYFIP1 antigen affinity purified monoclonal antibody (Catalog # M04596) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYFIP1 at approximately 145 kDa. The expected band size for CYFIP1 is at 145 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-exoc3-rabbit-monoclonal-antibody-m08984-2-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08984-2-wb.jpgAnti-EXOC3 Rabbit Monoclonal AntibodyWestern blot analysis of EXOC3 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acads-scad-rabbit-monoclonal-antibody-m05028-1-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05028-1-wb.jpgAnti-ACADS / SCAD Rabbit Monoclonal AntibodyWestern blot analysis of ACADS / SCAD expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05028-1-ip7.jpgAnti-ACADS / SCAD Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mas1l-rabbit-monoclonal-antibody-m16029-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16029-wb.jpgAnti-MAS1L Rabbit Monoclonal AntibodyWestern blot analysis of MAS1L expression in 293T cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16029-ip7.jpgAnti-MAS1L Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sf3a3-rabbit-monoclonal-antibody-m10671-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10671-wb.jpgAnti-SF3A3 Rabbit Monoclonal AntibodyWestern blot analysis of SF3A3 expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kif5b-rabbit-monoclonal-antibody-m01934-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01934-wb.jpgAnti-KIF5B Rabbit Monoclonal AntibodyWestern blot analysis of KIF5B expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd32a-cd32b-cd32c-rabbit-monoclonal-antibody-m01450-2-boster.html2026-03-17T05:13:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01450-2-wb.jpgAnti-CD32A + CD32B + CD32C Rabbit Monoclonal AntibodyWestern blot analysis of CD32A + CD32B + CD32C expression in U937 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-nucleolin-t76-rabbit-monoclonal-antibody-p00228-1-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00228-1-wb.jpgAnti-Phospho-Nucleolin (T76) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-Nucleolin (T76) expression in (1) 293T cell lysate; (2) 293T cell treated with Lambda Phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-syndecan-3-rabbit-monoclonal-antibody-m06245-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06245-wb.jpgAnti-Syndecan 3 Rabbit Monoclonal AntibodyWestern blot analysis of Syndecan 3 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jnk1-rabbit-monoclonal-antibody-m02608-3-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02608-3-mapk8-primary-antibodies-wb-testing-1_1.jpgAnti-JNK1 Rabbit Monoclonal Antibody Western blot analysis of JNK1/MAPK8 using anti-JNK1/MAPK8 antibody (M02608-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK1/MAPK8 antigen affinity purified monoclonal antibody (Catalog # M02608-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK1/MAPK8 at approximately 45 kDa. The expected band size for JNK1/MAPK8 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02608-3-mapk8-primary-antibodies-ihc-testing-2.jpgAnti-JNK1 Rabbit Monoclonal Antibody IHC analysis of JNK1/MAPK8 using anti-JNK1/MAPK8 antibody (M02608-3). JNK1/MAPK8 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JNK1/MAPK8 Antibody (M02608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02608-3-mapk8-primary-antibodies-ihc-testing-3.jpgAnti-JNK1 Rabbit Monoclonal Antibody IHC analysis of JNK1/MAPK8 using anti-JNK1/MAPK8 antibody (M02608-3). JNK1/MAPK8 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JNK1/MAPK8 Antibody (M02608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02608-3-mapk8-primary-antibodies-ihc-testing-4.jpgAnti-JNK1 Rabbit Monoclonal Antibody IHC analysis of JNK1/MAPK8 using anti-JNK1/MAPK8 antibody (M02608-3). JNK1/MAPK8 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JNK1/MAPK8 Antibody (M02608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02608-3-mapk8-primary-antibodies-ihc-testing-5.jpgAnti-JNK1 Rabbit Monoclonal Antibody IHC analysis of JNK1/MAPK8 using anti-JNK1/MAPK8 antibody (M02608-3). JNK1/MAPK8 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JNK1/MAPK8 Antibody (M02608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-mdm2-s166-rabbit-monoclonal-antibody-p00054-2-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-p-mdm2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyWestern blot analysis of P-MDM2 using anti-P-MDM2 antibody (P00054-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-MDM2 antigen affinity purified monoclonal antibody (P00054-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-MDM2 at approximately 100 kDa. The expected band size for P-MDM2 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-p-mdm2-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyIHC analysis of P-MDM2 using anti-P-MDM2 antibody (P00054-2). P-MDM2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-MDM2 Antibody (P00054-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-p-mdm2-primary-antibodies-ihc-testing-2.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyIHC analysis of P-MDM2 using anti-P-MDM2 antibody (P00054-2). P-MDM2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-MDM2 Antibody (P00054-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-p-mdm2-primary-antibodies-ihc-testing-3.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyIHC analysis of P-MDM2 using anti-P-MDM2 antibody (P00054-2). P-MDM2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-MDM2 Antibody (P00054-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-p-mdm2-primary-antibodies-ihc-testing-4.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyIHC analysis of P-MDM2 using anti-P-MDM2 antibody (P00054-2). P-MDM2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-MDM2 Antibody (P00054-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-p-mdm2-primary-antibodies-ihc-testing-5.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyIHC analysis of P-MDM2 using anti-P-MDM2 antibody (P00054-2). P-MDM2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-MDM2 Antibody (P00054-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-icc1_1.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-icc2_1.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:150 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-2-icc3_1.jpgAnti-Phospho-MDM2 (S166) Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:500 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pnk-pnkp-rabbit-monoclonal-antibody-m03566-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03566-wb.jpgAnti-PNK / PNKP Rabbit Monoclonal AntibodyWestern blot analysis of PNK / PNKP expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gnai2-rabbit-monoclonal-antibody-m02498-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02498-wb.jpgAnti-GNAI2 Rabbit Monoclonal AntibodyWestern blot analysis of GNAI2 expression in U-87 MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glyt2-rabbit-monoclonal-antibody-m03349-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03349-wb.jpgAnti-Glyt2 Rabbit Monoclonal AntibodyWestern blot analysis of Glyt2 expression in SH-SY5Y cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cep43-rabbit-monoclonal-antibody-m06907-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06907-wb.jpgAnti-CEP43 Rabbit Monoclonal AntibodyWestern blot analysis of CEP43 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mrps31-rabbit-monoclonal-antibody-m13714-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13714-wb.jpgAnti-MRPS31 Rabbit Monoclonal AntibodyWestern blot analysis of MRPS31 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc12a1-nkcc2-rabbit-monoclonal-antibody-m04473-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04473-wb.jpgAnti-SLC12A1 / NKCC2 Rabbit Monoclonal AntibodyWestern blot analysis of SLC12A1 / NKCC2 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ak2-rabbit-monoclonal-antibody-m04253-1-boster.html2026-03-17T05:13:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04253-1-wb.jpgAnti-AK2 Rabbit Monoclonal AntibodyWestern blot analysis of AK2 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c3orf38-rabbit-monoclonal-antibody-m17229-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m17229-wb.jpgAnti-C3orf38 Rabbit Monoclonal AntibodyWestern blot analysis of C3orf38 expression in U87-MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tsfm-rabbit-monoclonal-antibody-m08656-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08656-wb.jpgAnti-TSFM Rabbit Monoclonal AntibodyWestern blot analysis of TSFM expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myo19-rabbit-monoclonal-antibody-m10768-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10768-wb.jpgAnti-MYO19 Rabbit Monoclonal AntibodyWestern blot analysis of MYO19 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trim24-rabbit-monoclonal-antibody-m03258-1-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03258-1-wb.jpgAnti-TRIM24 Rabbit Monoclonal AntibodyWestern blot analysis of TRIM24 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wdr1-rabbit-monoclonal-antibody-m04814-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04814-wb.jpgAnti-WDR1 Rabbit Monoclonal AntibodyWestern blot analysis of WDR1 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbmxl2-rabbit-monoclonal-antibody-m15705-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m15705-wb.jpgAnti-RBMXL2 Rabbit Monoclonal AntibodyWestern blot analysis of RBMXL2 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m15705-ip7.jpgAnti-RBMXL2 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-integrin-beta-3-cd61-rabbit-monoclonal-antibody-m00587-2-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00587-2-wb.jpgAnti-Integrin beta 3 / CD61 Rabbit Monoclonal AntibodyWestern blot analysis of Integrin beta 3 / CD61 expression in U-87 MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-itpk1-rabbit-monoclonal-antibody-m09106-1-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09106-1-wb.jpgAnti-ITPK1 Rabbit Monoclonal AntibodyWestern blot analysis of ITPK1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-raver2-rabbit-monoclonal-antibody-m12771-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12771-wb.jpgAnti-RAVER2 Rabbit Monoclonal AntibodyWestern blot analysis of RAVER2 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufa1-rabbit-monoclonal-antibody-m08224-1-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08224-1-wb.jpgAnti-NDUFA1 Rabbit Monoclonal AntibodyWestern blot analysis of NDUFA1 expression in A673 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tapp1-rabbit-monoclonal-antibody-m08988-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08988-wb.jpgAnti-TAPP1 Rabbit Monoclonal AntibodyWestern blot analysis of TAPP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pyrophosphatase-1-rabbit-monoclonal-antibody-m07485-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07485-wb.jpgAnti-Pyrophosphatase 1 Rabbit Monoclonal AntibodyWestern blot analysis of Pyrophosphatase 1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acat1-rabbit-monoclonal-antibody-m02008-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02008-acat1-primary-antibodies-wb-testing-1.jpgAnti-ACAT1 Rabbit Monoclonal Antibody Western blot analysis of ACAT1 using anti-ACAT1 antibody (M02008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT1 antigen affinity purified monoclonal antibody (M02008) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAT1 at approximately 40 kDa. The expected band size for ACAT1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02008-ip7.jpgAnti-ACAT1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rpl10a-rabbit-monoclonal-antibody-m08606-1-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08606-1-wb.jpgAnti-RPL10A Rabbit Monoclonal AntibodyWestern blot analysis of RPL10A expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adss-2-rabbit-monoclonal-antibody-m07539-boster.html2026-03-17T05:13:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07539-wb.jpgAnti-AdSS 2 Rabbit Monoclonal AntibodyWestern blot analysis of AdSS 2 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gprc5b-rabbit-monoclonal-antibody-m08895-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08895-wb.jpgAnti-GPRC5B Rabbit Monoclonal AntibodyWestern blot analysis of GPRC5B expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ngr3-rabbit-monoclonal-antibody-m12234-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12234-wb.jpgAnti-NgR3 Rabbit Monoclonal AntibodyWestern blot analysis of NgR3 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dlx3-rabbit-monoclonal-antibody-m05844-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05844-wb7.jpgAnti-DLX3 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05844-wb.jpgAnti-DLX3 Rabbit Monoclonal AntibodyWestern blot analysis of DLX3 expression in JAR cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sft-rabbit-monoclonal-antibody-m04728-1-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04728-1-wb.jpgAnti-SFT Rabbit Monoclonal AntibodyWestern blot analysis of SFT expression in Raji cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04728-1-ube2d1-primary-antibodies-ihc-testing-1.jpgAnti-SFT Rabbit Monoclonal AntibodyIHC analysis of UBE2D1 using anti-UBE2D1 antibody (M04728-1). UBE2D1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-UBE2D1 Antibody (M04728-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04728-1-ip7.jpgAnti-SFT Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pah-rabbit-monoclonal-antibody-m00761-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00761-wb.jpgAnti-PAH Rabbit Monoclonal AntibodyWestern blot analysis of PAH expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uso1-rabbit-monoclonal-antibody-m05911-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05911-wb.jpgAnti-USO1 Rabbit Monoclonal AntibodyWestern blot analysis of USO1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nudt19-rabbit-monoclonal-antibody-m14864-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14864-wb.jpgAnti-NUDT19 Rabbit Monoclonal AntibodyWestern blot analysis of NUDT19 expression in 293T cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tor4a-rabbit-monoclonal-antibody-m17448-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m17448-wb.jpgAnti-TOR4A Rabbit Monoclonal AntibodyWestern blot analysis of TOR4A expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pex11b-rabbit-monoclonal-antibody-m08864-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08864-pex11b-primary-antibodies-wb-testing-1.jpgAnti-PEX11B Rabbit Monoclonal AntibodyWestern blot analysis of PEX11B using anti-PEX11B antibody (M08864). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human REH whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEX11B antigen affinity purified monoclonal antibody (M08864) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PEX11B at approximately 25 kDa. The expected band size for PEX11B is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08864-pex11b-primary-antibodies-ihc-testing-1.jpgAnti-PEX11B Rabbit Monoclonal AntibodyIHC analysis of PEX11B using anti-PEX11B antibody (M08864). PEX11B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PEX11B Antibody (M08864) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif1a-rabbit-monoclonal-antibody-m09001-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09001-wb.jpgAnti-eIF1A Rabbit Monoclonal AntibodyWestern blot analysis of eIF1A expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nup107-rabbit-monoclonal-antibody-m03724-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03724-wb.jpgAnti-Nup107 Rabbit Monoclonal AntibodyWestern blot analysis of Nup107 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-iqgap2-rabbit-monoclonal-antibody-m04787-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04787-iqgap2-primary-antibodies-wb-testing-1.jpgAnti-IQGAP2 Rabbit Monoclonal Antibody Western blot analysis of IQGAP2 using anti-IQGAP2 antibody (M04787). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse stomach tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP2 antigen affinity purified monoclonal antibody (Catalog # M04787) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP2 at approximately 181 kDa. The expected band size for IQGAP2 is at 181 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04787-iqgap2-primary-antibodies-ihc-testing-2.jpgAnti-IQGAP2 Rabbit Monoclonal Antibody IHC analysis of IQGAP2 using anti-IQGAP2 antibody (M04787). IQGAP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IQGAP2 Antibody (M04787) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pctaire1-rabbit-monoclonal-antibody-m06102-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06102-wb.jpgAnti-PCTAIRE1 Rabbit Monoclonal AntibodyWestern blot analysis of PCTAIRE1 expression in BxPC-3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-preb-rabbit-monoclonal-antibody-m04946-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04946-wb.jpgAnti-PREB Rabbit Monoclonal AntibodyWestern blot analysis of PREB expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mras-rabbit-monoclonal-antibody-m06332-1-boster.html2026-03-17T05:13:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06332-1-wb.jpgAnti-Mras Rabbit Monoclonal AntibodyWestern blot analysis of Mras expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppm1g-rabbit-monoclonal-antibody-m06725-1-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06725-1-wb.jpgAnti-PPM1G Rabbit Monoclonal AntibodyWestern blot analysis of PPM1G expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp5g1-rabbit-monoclonal-antibody-m32382-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32382-wb.jpgAnti-ATP5G1 Rabbit Monoclonal AntibodyWestern blot analysis of ATP5G1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp5g1-rabbit-monoclonal-antibody-m32382-1-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32382-1-wb.jpgAnti-ATP5G1 Rabbit Monoclonal AntibodyWestern blot analysis of ATP5G1 expression in HL-60 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-temt-rabbit-monoclonal-antibody-m09175-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09175-wb.jpgAnti-TEMT Rabbit Monoclonal AntibodyWestern blot analysis of TEMT expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hnrnpm-rabbit-monoclonal-antibody-m06017-1-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06017-1-hnrnpm-primary-antibodies-wb-testing-1.jpgAnti-HNRNPM Rabbit Monoclonal Antibody Western blot analysis of HNRNPM using anti-HNRNPM antibody (M06017-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPM antigen affinity purified monoclonal antibody (Catalog # M06017-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNRNPM at approximately 73 kDa. The expected band size for HNRNPM is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06017-1-hnrnpm-primary-antibodies-ihc-testing-2.jpgAnti-HNRNPM Rabbit Monoclonal Antibody IHC analysis of HNRNPM using anti-HNRNPM antibody (M06017-1). HNRNPM was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNRNPM Antibody (M06017-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06017-1-hnrnpm-primary-antibodies-ihc-testing-3.jpgAnti-HNRNPM Rabbit Monoclonal Antibody IHC analysis of HNRNPM using anti-HNRNPM antibody (M06017-1). HNRNPM was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNRNPM Antibody (M06017-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06017-1-hnrnpm-primary-antibodies-ihc-testing-4.jpgAnti-HNRNPM Rabbit Monoclonal Antibody IHC analysis of HNRNPM using anti-HNRNPM antibody (M06017-1). HNRNPM was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNRNPM Antibody (M06017-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06017-1-hnrnpm-primary-antibodies-ihc-testing-5.jpgAnti-HNRNPM Rabbit Monoclonal Antibody IHC analysis of HNRNPM using anti-HNRNPM antibody (M06017-1). HNRNPM was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNRNPM Antibody (M06017-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif1a-rabbit-monoclonal-antibody-m09001-1-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09001-1-wb.jpgAnti-eIF1A Rabbit Monoclonal AntibodyWestern blot analysis of eIF1A expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mst4-rabbit-monoclonal-antibody-m09552-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09552-stk26-primary-antibodies-wb-testing-1.jpgAnti-MST4 Rabbit Monoclonal AntibodyWestern blot analysis of MST4/STK26 using anti-MST4/STK26 antibody (M09552). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST4/STK26 antigen affinity purified monoclonal antibody (M09552) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MST4/STK26 at approximately 50 kDa. The expected band size for MST4/STK26 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09552-stk26-primary-antibodies-ihc-testing-1.jpgAnti-MST4 Rabbit Monoclonal AntibodyIHC analysis of MST4/STK26 using anti-MST4/STK26 antibody (M09552). MST4/STK26 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-MST4/STK26 Antibody (M09552) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ms4a14-rabbit-monoclonal-antibody-m15744-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m15744-wb.jpgAnti-MS4A14 Rabbit Monoclonal AntibodyWestern blot analysis of MS4A14 expression in MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-galt-rabbit-monoclonal-antibody-m01460-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01460-wb.jpgAnti-GALT Rabbit Monoclonal AntibodyWestern blot analysis of GALT expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kctd9-rabbit-monoclonal-antibody-m12515-1-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12515-1-wb.jpgAnti-KCTD9 Rabbit Monoclonal AntibodyWestern blot analysis of KCTD9 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mdh2-rabbit-monoclonal-antibody-m04803-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04803-wb.jpgAnti-MDH2 Rabbit Monoclonal AntibodyWestern blot analysis of MDH2 expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmc5-rabbit-monoclonal-antibody-m05480-1-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05480-1-wb.jpgAnti-PSMC5 Rabbit Monoclonal AntibodyWestern blot analysis of PSMC5 expression in A431 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05480-1-ip7.jpgAnti-PSMC5 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aamp-rabbit-monoclonal-antibody-m07409-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07409-wb.jpgAnti-AAMP Rabbit Monoclonal AntibodyWestern blot analysis of AAMP expression in (1) A375 lysate; (2) MCF7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-brat1-rabbit-monoclonal-antibody-m06265-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06265-wb.jpgAnti-BRAT1 Rabbit Monoclonal AntibodyWestern blot analysis of BRAT1 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06265-ip7.jpgAnti-BRAT1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dazap1-rabbit-monoclonal-antibody-m07947-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07947-wb.jpgAnti-DAZAP1 Rabbit Monoclonal AntibodyWestern blot analysis of DAZAP1 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcea1-rabbit-monoclonal-antibody-m08133-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08133-tcea1-primary-antibodies-wb-testing-1.jpgAnti-TCEA1 Rabbit Monoclonal AntibodyWestern blot analysis of TCEA1 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08133-tcea1-primary-antibodies-ihc-testing-2.jpgAnti-TCEA1 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded mouse large intestine tissue with TCEA1 antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08133-ip7.jpgAnti-TCEA1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-temt-rabbit-monoclonal-antibody-m09175-1-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09175-1-wb.jpgAnti-TEMT Rabbit Monoclonal AntibodyWestern blot analysis of TEMT expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fpgs-rabbit-monoclonal-antibody-m02836-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02836-wb.jpgAnti-FPGS Rabbit Monoclonal AntibodyWestern blot analysis of FPGS expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cecr5-rabbit-monoclonal-antibody-m31869-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31869-wb.jpgAnti-CECR5 Rabbit Monoclonal AntibodyWestern blot analysis of CECR5 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ift88-rabbit-monoclonal-antibody-m06814-boster.html2026-03-17T05:13:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06814-wb.jpgAnti-IFT88 Rabbit Monoclonal AntibodyWestern blot analysis of IFT88 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prmt7-rabbit-monoclonal-antibody-m05485-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05485-1-wb.jpgAnti-PRMT7 Rabbit Monoclonal AntibodyWestern blot analysis of PRMT7 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab8a-rabbit-monoclonal-antibody-m02180-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02180-wb.jpgAnti-RAB8A Rabbit Monoclonal AntibodyWestern blot analysis of RAB8A expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ufd1l-rabbit-monoclonal-antibody-m32358-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32358-1-wb.jpgAnti-UFD1L Rabbit Monoclonal AntibodyWestern blot analysis of UFD1L expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gne-rabbit-monoclonal-antibody-m01647-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01647-wb.jpgAnti-GNE Rabbit Monoclonal AntibodyWestern blot analysis of GNE expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prpf31-rabbit-monoclonal-antibody-m03137-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03137-wb.jpgAnti-PRPF31 Rabbit Monoclonal AntibodyWestern blot analysis of PRPF31 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-msrb3-rabbit-monoclonal-antibody-m06632-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06632-1-wb.jpgAnti-MSRB3 Rabbit Monoclonal AntibodyWestern blot analysis of MSRB3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ptprr-rabbit-monoclonal-antibody-m06905-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06905-ptprr-primary-antibodies-wb-testing-1.jpgAnti-PTPRR Rabbit Monoclonal AntibodyWestern blot analysis of PTPRR using anti-PTPRR antibody (M06905). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPRR antigen affinity purified monoclonal antibody (M06905) at 1:500 overnight at 4°C, then washed with TBS-10%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTPRR at approximately 70 kDa. The expected band size for PTPRR is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06905-ptprr-primary-antibodies-wb-testing-2_1.jpgAnti-PTPRR Rabbit Monoclonal AntibodyWestern blot analysis of PTPRR using anti-PTPRR antibody (M06905). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPRR antigen affinity purified monoclonal antibody (M06905) at 1:500 overnight at 4°C, then washed with TBS-10%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTPRR at approximately 70 kDa. The expected band size for PTPRR is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06905-ptprr-primary-antibodies-ihc-testing-2.jpgAnti-PTPRR Rabbit Monoclonal Antibody IHC analysis of PTPRR using anti-PTPRR antibody (M06905) . PTPRR was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPRR Antibody (M06905) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pnk-pnkp-rabbit-monoclonal-antibody-m03566-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03566-1-wb.jpgAnti-PNK / PNKP Rabbit Monoclonal AntibodyWestern blot analysis of PNK / PNKP expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nsdhl-rabbit-monoclonal-antibody-m04902-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04902-wb.jpgAnti-NSDHL Rabbit Monoclonal AntibodyWestern blot analysis of NSDHL expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aer61-rabbit-monoclonal-antibody-m09267-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09267-eogt-primary-antibodies-wb-testing-1.jpgAnti-AER61 Rabbit Monoclonal AntibodyWestern blot analysis of EOGT using anti-EOGT antibody (M09267). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EOGT antigen affinity purified monoclonal antibody (M09267) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EOGT at approximately 62 kDa. The expected band size for EOGT is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mld-rabbit-monoclonal-antibody-m05502-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05502-wb7.jpgAnti-MLD Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05502-wb.jpgAnti-MLD Rabbit Monoclonal AntibodyWestern blot analysis of MLD expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmem192-rabbit-monoclonal-antibody-m11938-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11938-wb.jpgAnti-TMEM192 Rabbit Monoclonal AntibodyWestern blot analysis of TMEM192 expression in U87-MG cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11938-wb7.jpgAnti-TMEM192 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11938-wb8.jpgAnti-TMEM192 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:4K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcea1-rabbit-monoclonal-antibody-m08133-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08133-1-wb.jpgAnti-TCEA1 Rabbit Monoclonal AntibodyWestern blot analysis of TCEA1 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08133-1-ip7.jpgAnti-TCEA1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppm1g-rabbit-monoclonal-antibody-m06725-2-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06725-2-wb.jpgAnti-PPM1G Rabbit Monoclonal AntibodyWestern blot analysis of PPM1G expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppp2r5d-rabbit-monoclonal-antibody-m06450-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06450-1-wb.jpgAnti-PPP2R5D Rabbit Monoclonal AntibodyWestern blot analysis of PPP2R5D expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06450-1-ip7.jpgAnti-PPP2R5D Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-iqgap2-rabbit-monoclonal-antibody-m04787-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04787-1-wb.jpgAnti-IQGAP2 Rabbit Monoclonal AntibodyWestern blot analysis of IQGAP2 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-safb-rabbit-monoclonal-antibody-m03458-1-boster.html2026-03-17T05:13:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03458-1-wb.jpgAnti-SAFB Rabbit Monoclonal AntibodyWestern blot analysis of SAFB expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-p130-t986-rabbit-monoclonal-antibody-p02118-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02118-wb.jpgAnti-Phospho-p130(T986) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-p130(T986) expression in (1) Jurkat cell lysate; (2) Jurkat cell treated with phosphatase lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-snx5-rabbit-monoclonal-antibody-m04788-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04788-wb.jpgAnti-SNX5 Rabbit Monoclonal AntibodyWestern blot analysis of SNX5 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-taf3-rabbit-monoclonal-antibody-m07129-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07129-wb.jpgAnti-TAF3 Rabbit Monoclonal AntibodyWestern blot analysis of TAF3 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-azi2-rabbit-monoclonal-antibody-m08235-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08235-wb7.jpgAnti-AZI2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08235-wb.jpgAnti-AZI2 Rabbit Monoclonal AntibodyWestern blot analysis of AZI2 expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08235-wb8.jpgAnti-AZI2 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08235-icc1.jpgAnti-AZI2 Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-septin-8-rabbit-monoclonal-antibody-m30757-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30757-wb.jpgAnti-Septin 8 Rabbit Monoclonal AntibodyWestern blot analysis of Septin 8 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mk3-rabbit-monoclonal-antibody-m04510-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04510-wb.jpgAnti-MK3 Rabbit Monoclonal AntibodyWestern blot analysis of MK3 expression in Jurkat cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04510-ip7.jpgAnti-MK3 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myo1b-rabbit-monoclonal-antibody-m07011-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07011-myo1b-primary-antibodies-wb-testing-1.jpgAnti-MYO1B Rabbit Monoclonal AntibodyWestern blot analysis of MYO1B using anti-MYO1B antibody (M07011). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYO1B antigen affinity purified monoclonal antibody (M07011) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYO1B at approximately 132 kDa. The expected band size for MYO1B is at 132 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07011-myo1b-primary-antibodies-ihc-testing-1.jpgAnti-MYO1B Rabbit Monoclonal AntibodyIHC analysis of MYO1B using anti-MYO1B antibody (M07011). MYO1B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO1B Antibody (M07011) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nth1-rabbit-monoclonal-antibody-m03207-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03207-wb.jpgAnti-NTH1 Rabbit Monoclonal AntibodyWestern blot analysis of NTH1 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acap2-rabbit-monoclonal-antibody-m09294-1-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09294-1-wb.jpgAnti-ACAP2 Rabbit Monoclonal AntibodyWestern blot analysis of ACAP2 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tns4-rabbit-monoclonal-antibody-m07240-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07240-wb.jpgAnti-TNS4 Rabbit Monoclonal AntibodyWestern blot analysis of TNS4 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pcbp2-rabbit-monoclonal-antibody-m02425-1-boster.html2026-03-24T05:36:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-1-pcbp2-primary-antibodies-wb-testing-1.jpgAnti-PCBP2 Rabbit Monoclonal AntibodyWestern blot analysis of PCBP2 using anti-PCBP2 antibody (M02425-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBP2 antigen affinity purified monoclonal antibody (M02425-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCBP2 at approximately 39 kDa. The expected band size for PCBP2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-1-pcbp2-primary-antibodies-ihc-testing-1.jpgAnti-PCBP2 Rabbit Monoclonal AntibodyIHC analysis of PCBP2 using anti-PCBP2 antibody (M02425-1). PCBP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PCBP2 Antibody (M02425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-1-pcbp2-primary-antibodies-ihc-testing-2.jpgAnti-PCBP2 Rabbit Monoclonal AntibodyIHC analysis of PCBP2 using anti-PCBP2 antibody (M02425-1). PCBP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PCBP2 Antibody (M02425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-1-pcbp2-primary-antibodies-ihc-testing-3.jpgAnti-PCBP2 Rabbit Monoclonal AntibodyIHC analysis of PCBP2 using anti-PCBP2 antibody (M02425-1). PCBP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-PCBP2 Antibody (M02425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02425-1-ip7.jpgAnti-PCBP2 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pgm1-rabbit-monoclonal-antibody-m02318-1-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02318-1-wb.jpgAnti-PGM1 Rabbit Monoclonal AntibodyWestern blot analysis of PGM1 expression in (1) 293 cell lysate; (2) RAW 264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tnfaip8-rabbit-monoclonal-antibody-m05158-1-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05158-1-wb.jpgAnti-TNFAIP8 Rabbit Monoclonal AntibodyWestern blot analysis of TNFAIP8 expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cfd-rabbit-monoclonal-antibody-m01134-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01134-wb.jpgAnti-CFD Rabbit Monoclonal AntibodyWestern blot analysis of CFD expression in THP-1 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clic4-rabbit-monoclonal-antibody-m03210-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03210-clic4-primary-antibodies-wb-testing-1.jpgAnti-CLIC4 Rabbit Monoclonal Antibody Western blot analysis of CLIC4 using anti-CLIC4 antibody (M03210). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC4 antigen affinity purified monoclonal antibody (Catalog # M03210) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIC4 at approximately 29 kDa. The expected band size for CLIC4 is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmb10-rabbit-monoclonal-antibody-m04960-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04960-wb.jpgAnti-PSMB10 Rabbit Monoclonal AntibodyWestern blot analysis of PSMB10 expression in Raji cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf115-rabbit-monoclonal-antibody-m09300-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09300-wb.jpgAnti-RNF115 Rabbit Monoclonal AntibodyWestern blot analysis of RNF115 expression in PC-3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ufc1-rabbit-monoclonal-antibody-m06160-boster.html2026-03-17T05:13:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06160-wb.jpgAnti-UFC1 Rabbit Monoclonal AntibodyWestern blot analysis of UFC1 expression in A549 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prd-rabbit-monoclonal-antibody-m03417-2-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03417-2-wb.jpgAnti-PRD Rabbit Monoclonal AntibodyWestern blot analysis of PRD expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-taok1-rabbit-monoclonal-antibody-m07063-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07063-wb.jpgAnti-TAOK1 Rabbit Monoclonal AntibodyWestern blot analysis of TAOK1 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmem43-rabbit-monoclonal-antibody-m05893-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05893-tmem43-primary-antibodies-wb-testing-1_1.jpgAnti-TMEM43 Rabbit Monoclonal Antibody Western blot analysis of TMEM43 using anti-TMEM43 antibody (M05893). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM43 antigen affinity purified monoclonal antibody (Catalog # M05893) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM43 at approximately 45 kDa. The expected band size for TMEM43 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nolc1-rabbit-monoclonal-antibody-m04778-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04778-nolc1-primary-antibodies-wb-testing-1.jpgAnti-NOLC1 Rabbit Monoclonal Antibody Western blot analysis of NOLC1 using anti-NOLC1 antibody (M04778). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOLC1 antigen affinity purified monoclonal antibody (M04778) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOLC1 at approximately 130 kDa. The expected band size for NOLC1 is at 74 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-emc4-rabbit-monoclonal-antibody-m13212-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13212-wb.jpgAnti-EMC4 Rabbit Monoclonal AntibodyWestern blot analysis of EMC4 expression in 293 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13212-ip7.jpgAnti-EMC4 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-pbk-topk-thr9-rabbit-monoclonal-antibody-p02623-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02623-wb.jpgAnti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-PBK/TOPK (Thr9) expression in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-homer1-rabbit-monoclonal-antibody-m03877-1-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03877-1-homer1-primary-antibodies-wb-testing-1.jpgAnti-Homer1 Rabbit Monoclonal AntibodyWestern blot analysis of HOMER1 using anti-HOMER1 antibody (M03877-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOMER1 antigen affinity purified monoclonal antibody (M03877-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HOMER1 at approximately 45 kDa. The expected band size for HOMER1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03877-1-12672_2025_3528_fig9_html_1.pngAnti-Homer1 Rabbit Monoclonal AntibodyA – H HOMER1 knockout efficiency was verified by quantitative PCR (qPCR) and western blotting after transfection of siRNA in Huh7 and SNU-449 cells. CCK8 assay ( I ) and colony formation assay ( J – L ) were used to verify the effect of down-regulation of HOMER1 expression on cell proliferation. The effect of HOMER1 knockdown on the migration ability of Huh7 and SNU-449 cells was evaluated by wound healing assay ( M – O ). The effects of down-regulation of HOMER1 expression on Huh7 and SNU-449 cell migration ( P – R ) and invasion ability ( S – T ) were analyzed by transwell assay. (* p < 0.05, ** p < 0.005, *** p < 0.001.) Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s12672-025-03528-6'>41065971</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03877-1-12672_2025_3528_fig9_html.pngAnti-Homer1 Rabbit Monoclonal AntibodyA – H HOMER1 knockout efficiency was verified by quantitative PCR (qPCR) and western blotting after transfection of siRNA in Huh7 and SNU-449 cells. CCK8 assay ( I ) and colony formation assay ( J – L ) were used to verify the effect of down-regulation of HOMER1 expression on cell proliferation. The effect of HOMER1 knockdown on the migration ability of Huh7 and SNU-449 cells was evaluated by wound healing assay ( M – O ). The effects of down-regulation of HOMER1 expression on Huh7 and SNU-449 cell migration ( P – R ) and invasion ability ( S – T ) were analyzed by transwell assay. (* p < 0.05, ** p < 0.005, *** p < 0.001.) Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s12672-025-03528-6'>41065971</a> https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnaja4-rabbit-monoclonal-antibody-m10527-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10527-wb.jpgAnti-DNAJA4 Rabbit Monoclonal AntibodyWestern blot analysis of DNAJA4 expression in Hela cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10527-ip7.jpgAnti-DNAJA4 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10527-wb7.jpgAnti-DNAJA4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10527-wb8.jpgAnti-DNAJA4 Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fam50a-rabbit-monoclonal-antibody-m12622-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12622-fam50a-primary-antibodies-wb-testing-1_1.jpgAnti-FAM50A Rabbit Monoclonal Antibody Western blot analysis of FAM50A using anti-FAM50A antibody (M12622). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM50A antigen affinity purified monoclonal antibody (Catalog # M12622) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM50A at approximately 40 kDa. The expected band size for FAM50A is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12622-fam50a-primary-antibodies-ihc-testing-2.jpgAnti-FAM50A Rabbit Monoclonal Antibody IHC analysis of FAM50A using anti-FAM50A antibody (M12622). FAM50A was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAM50A Antibody (M12622) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12622-fam50a-primary-antibodies-ihc-testing-3.jpgAnti-FAM50A Rabbit Monoclonal Antibody IHC analysis of FAM50A using anti-FAM50A antibody (M12622). FAM50A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAM50A Antibody (M12622) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12622-fam50a-primary-antibodies-ihc-testing-4.jpgAnti-FAM50A Rabbit Monoclonal Antibody IHC analysis of FAM50A using anti-FAM50A antibody (M12622). FAM50A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FAM50A Antibody (M12622) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nesprin3-rabbit-monoclonal-antibody-m13164-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13164-wb.jpgAnti-Nesprin3 Rabbit Monoclonal AntibodyWestern blot analysis of Nesprin3 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uchl5ip-rabbit-monoclonal-antibody-m12338-1-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12338-1-wb.jpgAnti-UCHL5IP Rabbit Monoclonal AntibodyWestern blot analysis of UCHL5IP expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nesprin3-rabbit-monoclonal-antibody-m13164-1-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13164-1-wb.jpgAnti-Nesprin3 Rabbit Monoclonal AntibodyWestern blot analysis of Nesprin3 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tagap-rabbit-monoclonal-antibody-m09403-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09403-tagap-primary-antibodies-wb-testing-1.jpgAnti-TAGAP Rabbit Monoclonal Antibody Western blot analysis of TAGAP using anti-TAGAP antibody (M09403). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGAP antigen affinity purified monoclonal antibody (Catalog # M09403) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGAP at approximately 72 kDa. The expected band size for TAGAP is at 81 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmb10-rabbit-monoclonal-antibody-m04960-1-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04960-1-wb.jpgAnti-PSMB10 Rabbit Monoclonal AntibodyWestern blot analysis of PSMB10 expression in Daudi cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sec24d-rabbit-monoclonal-antibody-m06985-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06985-wb.jpgAnti-SEC24D Rabbit Monoclonal AntibodyWestern blot analysis of SEC24D expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pwp1-rabbit-monoclonal-antibody-m10720-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10720-wb.jpgAnti-PWP1 Rabbit Monoclonal AntibodyWestern blot analysis of PWP1 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufs2-rabbit-monoclonal-antibody-m05618-1-boster.html2026-03-17T05:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05618-1-ndufs2-primary-antibodies-wb-testing-1.jpgAnti-NDUFS2 Rabbit Monoclonal Antibody Western blot analysis of NDUFS2 using anti-NDUFS2 antibody (M05618-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human TE-1 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS2 antigen affinity purified monoclonal antibody (M05618-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS2 at approximately 43 kDa. The expected band size for NDUFS2 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05618-1-nduf-s2-primary-antibodies-ihc-testing-2.jpgAnti-NDUFS2 Rabbit Monoclonal Antibody IHC analysis of NDUF-S2 using anti-NDUF-S2 antibody (M05618-1). NDUF-S2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUF-S2 Antibody (M05618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05618-1-nduf-s2-primary-antibodies-ihc-testing-3.jpgAnti-NDUFS2 Rabbit Monoclonal Antibody IHC analysis of NDUF-S2 using anti-NDUF-S2 antibody (M05618-1). NDUF-S2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUF-S2 Antibody (M05618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05618-1-nduf-s2-primary-antibodies-ihc-testing-4.jpgAnti-NDUFS2 Rabbit Monoclonal Antibody IHC analysis of NDUF-S2 using anti-NDUF-S2 antibody (M05618-1). NDUF-S2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUF-S2 Antibody (M05618-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufb4-rabbit-monoclonal-antibody-m11016-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11016-ndufb4-primary-antibodies-wb-testing-1.jpgAnti-NDUFB4 Rabbit Monoclonal Antibody Western blot analysis of NDUFB4 using anti-NDUFB4 antibody (M11016). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB4 antigen affinity purified monoclonal antibody (M11016) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB4 at approximately 15 kDa. The expected band size for NDUFB4 is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufc2-rabbit-monoclonal-antibody-m09084-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09084-wb.jpgAnti-NDUFC2 Rabbit Monoclonal AntibodyWestern blot analysis of NDUFC2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uchl5ip-rabbit-monoclonal-antibody-m12338-2-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12338-2-wb.jpgAnti-UCHL5IP Rabbit Monoclonal AntibodyWestern blot analysis of UCHL5IP expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab45-rabbit-monoclonal-antibody-m10871-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10871-wb.jpgAnti-RAB45 Rabbit Monoclonal AntibodyWestern blot analysis of RAB45 expression in (1) A431 cell lysate; (2) Raw264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grsf1-rabbit-monoclonal-antibody-m07576-1-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07576-1-grsf1-primary-antibodies-wb-testing-1.jpgAnti-GRSF1 Rabbit Monoclonal AntibodyWestern blot analysis of GRSF1 using anti-GRSF1 antibody (M07576-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRSF1 antigen affinity purified monoclonal antibody (M07576-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRSF1 at approximately 53 kDa. The expected band size for GRSF1 is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmgcl-rabbit-monoclonal-antibody-m01886-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01886-wb.jpgAnti-HMGCL Rabbit Monoclonal AntibodyWestern blot analysis of HMGCL expression in A431 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-annexin-a7-rabbit-monoclonal-antibody-m04889-1-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04889-1-wb.jpgAnti-Annexin A7 Rabbit Monoclonal AntibodyWestern blot analysis of Annexin A7 expression in (1) Jurkat cell lysate; (2) Raw264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prpf4-rabbit-monoclonal-antibody-m08225-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08225-wb.jpgAnti-PRPF4 Rabbit Monoclonal AntibodyWestern blot analysis of PRPF4 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pi3-kinase-p110-beta-rabbit-monoclonal-antibody-m01091-1-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-wb7.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-fphar-16-1582677-g005.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyBECCs regulate PI3K, PDPK1, and mTOR protein levels in HAPH rats. (A–C) Primitive bands of p-PI3K, PI3K, p-PDPK1, PDPK1, p-mTOR, and mTOR by Western blots in lung tissues. (D–F) Quantitative evaluation of p-PI3K, PI3K, p-PDPK1, PDPK1, p-mTOR, and mTOR in lung tissues. n = 5. All data are represented as the mean ± SD. * p < 0.05 vs. control group and # p < 0.05 vs. hypoxia group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-fphar-16-1582677-g007.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyFlavonoids inhibit the proliferation of PASMCs under hypoxic conditions by inhibiting the PI3K/AKT axis. (A, B) Primitive bands and quantitative densities of p-AKT1 Ser473 and AKT1 with or without Sc79 (20 μM) by Western blots in PASMCs under 3% O 2 . (C, D) Primitive bands and quantitative densities of p-PI3K, PI3K, p-AKT1 Ser473, and AKT1 with or without 740Y-P (10 μM) by Western blots in PASMCs under 3% O 2 . (E, F) Primitive bands and quantitative densities of p-PDPK1, PDPK, p-AKT1 Ser473, and AKT1 with or without MYH1485 (2 μM) in PASMCs by Western blots under 3% O 2 . n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + Fla-50 μg/ml group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-fphar-16-1582677-g008.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyEriocitrin and quercetin are responsible for anti-proliferation by targeting the PI3K protein in PASMCs under hypoxic conditions. ERI, eriocitrin; QCT, quercetin. (A, B) Primitive bands and quantitative evaluation of p-mTOR, mTOR, p-AKT1 (Ser473), and AKT1 with or without PS210 (2 μM) by Western blotting in PASMCs under 3% O 2 . n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + FLA-50 μg/ml group. (C–G) Primitive bands and quantitative densities of p-PI3K and PI3K by Western blots. n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group and # p < 0.05 vs. 3% O 2 group. (H–N) BECC, ERI, and QCT treatment increased the stability of PI3K in PASMC protease lysates by the DARTS experiment. (H–K) Primitive Western blots of PI3K. (L–N) Quantitative evaluation of PI3K levels. n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. DMSO group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-fphar-16-1582677-g009.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyTreatment with eriocitrin in PASMC proliferation and antioxidation under hypoxic conditions. (A, B) Eriocitrin in cell proliferation were assessed using Ki67 immunofluorescence and quantitative evaluation in hypoxia-induced PASMCs (n = 3, scale bar = 100 μm). (C–H) Primitive Western blots and quantitative densities of PCNA, p-PI3K, PI3K, p-AKT1 (Ser473), AKT1 with or without 740Y-P (10 μM), LY294002 (10 μM), or eriocitrin (11 μM) in PASMCs under 3% O 2 for 24 h (I–L) Quantitative evaluation of SOD and GSH-Px activities and GSH and MDA contents in 3% O 2 -induced PASMCs. n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + ERI-11 μM. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-fphar-16-1582677-g010.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyTreatment with quercetin in PASMC proliferation and antioxidation under hypoxia. (A, B) Quercetin in cell proliferation were assessed using Ki67 immunofluorescence and quantitative evaluation in hypoxia-induced PASMCs (n = 3, scale bar = 100 μm). (C–H) Primitive Western blots and quantitative densities of PCNA, p-PI3K, PI3K, p-AKT1 Ser473, AKT1 with or without 740Y-P(10 μM), LY294002(10 μM), or quercetin (18 μM) in PASMCs under 3% O 2 for 24 h (I–L) Quantitative evaluation of SOD and GSH-Px activities and GSH and MDA contents in 3% O 2 -induced PASMCs. n = 3. All data represent mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + QCT-18 μM. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-wb8.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-wb.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyWestern blot analysis of PI3 Kinase p110 beta expression in (1) 293 cell lysate; (2) Mouse Brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-1-pik3cb-primary-antibodies-ihc-testing-1.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyIHC analysis of PI3 Kinase p110 Beta/PIK3CB using anti-PI3 Kinase p110 Beta/PIK3CB antibody (M01091-1). PI3 Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PI3 Kinase p110 Beta/PIK3CB Antibody (M01091-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-1-pik3cb-primary-antibodies-ihc-testing-2.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyIHC analysis of PI3 Kinase p110 Beta/PIK3CB using anti-PI3 Kinase p110 Beta/PIK3CB antibody (M01091-1). PI3 Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PI3 Kinase p110 Beta/PIK3CB Antibody (M01091-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-1-pik3cb-primary-antibodies-ihc-testing-3.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyIHC analysis of PI3 Kinase p110 Beta/PIK3CB using anti-PI3 Kinase p110 Beta/PIK3CB antibody (M01091-1). PI3 Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PI3 Kinase p110 Beta/PIK3CB Antibody (M01091-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01091-1-1-pik3cb-primary-antibodies-ihc-testing-4.jpgAnti-PI3 Kinase p110 beta Rabbit Monoclonal AntibodyIHC analysis of PI3 Kinase p110 Beta/PIK3CB using anti-PI3 Kinase p110 Beta/PIK3CB antibody (M01091-1). PI3 Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PI3 Kinase p110 Beta/PIK3CB Antibody (M01091-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-snx4-rabbit-monoclonal-antibody-m08783-1-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08783-1-wb.jpgAnti-SNX4 Rabbit Monoclonal AntibodyWestern blot analysis of SNX4 expression in (1) A431 cell lysate; (2) Raw264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppp2r5e-rabbit-monoclonal-antibody-m07588-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07588-wb.jpgAnti-PPP2R5E Rabbit Monoclonal AntibodyWestern blot analysis of PPP2R5E expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nup155-rabbit-monoclonal-antibody-m06391-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06391-wb.jpgAnti-NUP155 Rabbit Monoclonal AntibodyWestern blot analysis of NUP155 expression in 293 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crcp-rabbit-monoclonal-antibody-m04247-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04247-wb.jpgAnti-CRCP Rabbit Monoclonal AntibodyWestern blot analysis of CRCP expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04247-crcp-primary-antibodies-wb-testing-1.pngAnti-CRCP Rabbit Monoclonal Antibody Western blot analysis of CRCP using anti-CRCP antibody (M04247). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: 2h drug treated-human MCF-7 whole cell lysates, Lane 3: 4h drug treated-human MCF-7 whole cell lysates, Lane 4: 12h drug treated-human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRCP antigen affinity purified monoclonal antibody (Catalog # M04247) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for CRCP at approximately 17kDa. The expected band size for CRCP is at 16.9 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppp2r5e-rabbit-monoclonal-antibody-m07588-1-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07588-1-wb.jpgAnti-PPP2R5E Rabbit Monoclonal AntibodyWestern blot analysis of PPP2R5E expression in 293 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07588-1-ip7.jpgAnti-PPP2R5E Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-actr1b-rabbit-monoclonal-antibody-m12745-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12745-wb.jpgAnti-ACTR1B Rabbit Monoclonal AntibodyWestern blot analysis of ACTR1B expression in 293 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12745-ip7.jpgAnti-ACTR1B Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prpf4-rabbit-monoclonal-antibody-m08225-1-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08225-1-wb.jpgAnti-PRPF4 Rabbit Monoclonal AntibodyWestern blot analysis of PRPF4 expression in (1) Raji cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phospho-c-jun-s73-jund-s100-rabbit-monoclonal-antibody-p02038-2-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02038-2-wb.jpgAnti-Phospho-c-Jun (S73)+JunD (S100) Rabbit Monoclonal AntibodyWestern blot analysis of Phospho-c-Jun (S73)+JunD (S100) expression in RAW 264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ate1-rabbit-monoclonal-antibody-m07459-boster.html2026-03-17T05:13:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07459-wb.jpgAnti-ATE1 Rabbit Monoclonal AntibodyWestern blot analysis of ATE1 expression in (1) HepG2 cell lysate; (2) Mouse spleen lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pmf1-rabbit-monoclonal-antibody-m08465-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08465-wb.jpgAnti-PMF1 Rabbit Monoclonal AntibodyWestern blot analysis of PMF1 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adiponectin-rabbit-monoclonal-antibody-m00509-3-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-3-wb.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyWestern blot analysis of Adiponectin expression in mouse kidney cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ssx2ip-rabbit-monoclonal-antibody-m05918-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05918-1-wb.jpgAnti-SSX2IP Rabbit Monoclonal AntibodyWestern blot analysis of SSX2IP expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nsdhl-rabbit-monoclonal-antibody-m04902-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04902-1-nsdhl-primary-antibodies-wb-testing-1.jpgAnti-NSDHL Rabbit Monoclonal Antibody Western blot analysis of NSDHL using anti-NSDHL antibody (M04902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSDHL antigen affinity purified monoclonal antibody (M04902-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSDHL at approximately 38 kDa. The expected band size for NSDHL is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04902-1-ip7.jpgAnti-NSDHL Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc25a12-rabbit-monoclonal-antibody-m04746-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04746-slc25a12-primary-antibodies-wb-testing-1.jpgAnti-SLC25A12 Rabbit Monoclonal AntibodyWestern blot analysis of SLC25A12 using anti-SLC25A12 antibody (M04746). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A12 antigen affinity purified monoclonal antibody (M04746) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A12 at approximately 70 kDa. The expected band size for SLC25A12 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04746-slc25a12-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A12 Rabbit Monoclonal AntibodyIHC analysis of SLC25A12 using anti-SLC25A12 antibody (M04746). SLC25A12 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SLC25A12 Antibody (M04746) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04746-slc25a12-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A12 Rabbit Monoclonal AntibodyIHC analysis of SLC25A12 using anti-SLC25A12 antibody (M04746). SLC25A12 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SLC25A12 Antibody (M04746) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clusterin-rabbit-monoclonal-antibody-m00590-3-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00590-3-wb.jpgAnti-Clusterin Rabbit Monoclonal AntibodyWestern blot analysis of Clusterin expression in mouse serum cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00590-3-ip7.jpgAnti-Clusterin Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:500 dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adiponectin-rabbit-monoclonal-antibody-m00509-4-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-4-wb.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyWestern blot analysis of Adiponectin expression in human plasma lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tim44-rabbit-monoclonal-antibody-m10278-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10278-wb.jpgAnti-TIM44 Rabbit Monoclonal AntibodyWestern blot analysis of TIM44 expression in (1) K562 cell lysate; (2) C6 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc27a4-rabbit-monoclonal-antibody-m05299-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05299-1-wb.jpgAnti-SLC27A4 Rabbit Monoclonal AntibodyWestern blot analysis of SLC27A4 expression in HepG2 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05299-1-ip7.jpgAnti-SLC27A4 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rps8-rabbit-monoclonal-antibody-m07839-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07839-rps8-primary-antibodies-wb-testing-1.jpgAnti-RPS8 Rabbit Monoclonal Antibody Western blot analysis of RPS8 using anti-RPS8 antibody (M07839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS8 antigen affinity purified monoclonal antibody (M07839) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS8 at approximately 26 kDa. The expected band size for RPS8 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07839-ip7.jpgAnti-RPS8 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-serine-racemase-rabbit-monoclonal-antibody-m02660-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02660-1-wb.jpgAnti-Serine racemase Rabbit Monoclonal AntibodyWestern blot analysis of Serine racemase expression in (1) 293 cell lysate; (2) U-87 MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gnaq-rabbit-monoclonal-antibody-m00898-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-1-gnaq-primary-antibodies-wb-testing-1.jpgAnti-GNAQ Rabbit Monoclonal AntibodyWestern blot analysis of GNAQ using anti-GNAQ antibody (M00898-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAQ antigen affinity purified monoclonal antibody (M00898-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNAQ at approximately 40 kDa. The expected band size for GNAQ is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-1-icc1.jpgAnti-GNAQ Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-1-gnaq-primary-antibodies-ihc-testing-1.jpgAnti-GNAQ Rabbit Monoclonal AntibodyIHC analysis of GNAQ using anti-GNAQ antibody (M00898-1). GNAQ was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GNAQ Antibody (M00898-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00898-1-gnaq-primary-antibodies-ihc-testing-2.jpgAnti-GNAQ Rabbit Monoclonal AntibodyIHC analysis of GNAQ using anti-GNAQ antibody (M00898-1). GNAQ was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-GNAQ Antibody (M00898-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-annexin-a7-rabbit-monoclonal-antibody-m04889-2-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04889-2-wb.jpgAnti-Annexin A7 Rabbit Monoclonal AntibodyWestern blot analysis of Annexin A7 expression in (1) Jurkat cell lysate; (2) Rat kidney lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-csde1-rabbit-monoclonal-antibody-m05114-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05114-wb.jpgAnti-CSDE1 Rabbit Monoclonal AntibodyWestern blot analysis of CSDE1 expression in (1) K562 cell lysate; (2) PC-12 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-meckelin-rabbit-monoclonal-antibody-m04691-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04691-wb7.jpgAnti-Meckelin Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04691-wb.jpgAnti-Meckelin Rabbit Monoclonal AntibodyWestern blot analysis of Meckelin expression in (1) MCF7 cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prepl-rabbit-monoclonal-antibody-m08771-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08771-wb.jpgAnti-PREPL Rabbit Monoclonal AntibodyWestern blot analysis of PREPL expression in (1) MCF7 cell lysate; (2) Raw264.7 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08771-ip7.jpgAnti-PREPL Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prepl-rabbit-monoclonal-antibody-m08771-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08771-1-wb.jpgAnti-PREPL Rabbit Monoclonal AntibodyWestern blot analysis of PREPL expression in (1) MCF7 cell lysate; (2) C6 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd147-rabbit-monoclonal-antibody-m00248-7-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00248-7-wb.jpgAnti-CD147 Rabbit Monoclonal AntibodyWestern blot analysis of CD147 expression in RAW 264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crabp2-rabbit-monoclonal-antibody-m03297-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03297-1-wb.jpgAnti-CRABP2 Rabbit Monoclonal AntibodyWestern blot analysis of CRABP2 expression in (1) MCF7 cell lysate; (2) Mouse skin lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-csde1-rabbit-monoclonal-antibody-m05114-1-boster.html2026-03-17T05:13:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05114-1-wb.jpgAnti-CSDE1 Rabbit Monoclonal AntibodyWestern blot analysis of CSDE1 expression in (1) 293 cell lysate; (2) Mouse brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05114-1-ip7.jpgAnti-CSDE1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcm6-rabbit-monoclonal-antibody-m02755-3-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02755-3-wb.jpgAnti-MCM6 Rabbit Monoclonal AntibodyWestern blot analysis of MCM6 expression in (1) MCF7 cell lysate; (2) NIH/3T3 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tctex1-rabbit-monoclonal-antibody-m05556-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05556-wb.jpgAnti-TCTEX1 Rabbit Monoclonal AntibodyWestern blot analysis of TCTEX1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-elovl5-rabbit-monoclonal-antibody-m03615-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03615-wb.jpgAnti-ELOVL5 Rabbit Monoclonal AntibodyWestern blot analysis of ELOVL5 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nucleolin-rabbit-monoclonal-antibody-m00228-3-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00228-3-wb.jpgAnti-Nucleolin Rabbit Monoclonal AntibodyWestern blot analysis of Nucleolin expression in K562 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tceb2-rabbit-monoclonal-antibody-m31718-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31718-wb.jpgAnti-TCEB2 Rabbit Monoclonal AntibodyWestern blot analysis of TCEB2 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmf-rabbit-monoclonal-antibody-m02214-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02214-wb.jpgAnti-TMF Rabbit Monoclonal AntibodyWestern blot analysis of TMF expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tceb2-rabbit-monoclonal-antibody-m31718-1-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31718-1-wb.jpgAnti-TCEB2 Rabbit Monoclonal AntibodyWestern blot analysis of TCEB2 expression in Jurkat cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ulbp1-rabbit-monoclonal-antibody-m04323-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04323-wb.jpgAnti-ULBP1 Rabbit Monoclonal AntibodyWestern blot analysis of ULBP1 expression in HeLa cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf7-rabbit-monoclonal-antibody-m05620-1-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05620-1-wb.jpgAnti-RNF7 Rabbit Monoclonal AntibodyWestern blot analysis of RNF7 expression in HepG2 cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bin1-rabbit-monoclonal-antibody-m01551-3-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01551-3-wb.jpgAnti-BIN1 Rabbit Monoclonal AntibodyWestern blot analysis of BIN1 expression in U87-MG cell lysate. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bin1-rabbit-monoclonal-antibody-m01551-4-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01551-4-wb.jpgAnti-BIN1 Rabbit Monoclonal AntibodyWestern blot analysis of BIN1 expression in A431 cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01551-4-ip7.jpgAnti-BIN1 Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufab1-rabbit-monoclonal-antibody-m09620-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09620-ndufab1-primary-antibodies-wb-testing-1.jpgAnti-NDUFAB1 Rabbit Monoclonal Antibody Western blot analysis of NDUFAB1 using anti-NDUFAB1 antibody (M09620). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAB1 antigen affinity purified monoclonal antibody (Catalog # M09620) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAB1 at approximately 14 kDa. The expected band size for NDUFAB1 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09620-ndufab1-primary-antibodies-ihc-testing-2.jpgAnti-NDUFAB1 Rabbit Monoclonal Antibody IHC analysis of NDUFAB1 using anti-NDUFAB1 antibody (MM09620). NDUFAB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 Antibody (M09620) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09620-ndufab1-primary-antibodies-ihc-testing-3.jpgAnti-NDUFAB1 Rabbit Monoclonal Antibody IHC analysis of NDUFAB1 using anti-NDUFAB1 antibody (MM09620). NDUFAB1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 Antibody (M09620) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09620-ndufab1-primary-antibodies-ihc-testing-4.jpgAnti-NDUFAB1 Rabbit Monoclonal Antibody IHC analysis of NDUFAB1 using anti-NDUFAB1 antibody (MM09620). NDUFAB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 Antibody (M09620) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09620-ndufab1-primary-antibodies-ihc-testing-5.jpgAnti-NDUFAB1 Rabbit Monoclonal Antibody IHC analysis of NDUFAB1 using anti-NDUFAB1 antibody (MM09620). NDUFAB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 Antibody (M09620) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09620-ndufab1-primary-antibodies-ihc-testing-6.jpgAnti-NDUFAB1 Rabbit Monoclonal Antibody IHC analysis of NDUFAB1 using anti-NDUFAB1 antibody (MM09620). NDUFAB1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 Antibody (M09620) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nmi-rabbit-monoclonal-antibody-m02768-2-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-2-wb.jpgAnti-NMI Rabbit Monoclonal AntibodyWestern blot analysis of NMI expression in HeLa cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02768-2-ip7.jpgAnti-NMI Rabbit Monoclonal AntibodyImmunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution) https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caspase-3-rabbit-monoclonal-antibody-m00334-9-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-9-casp3-primary-antibodies-wb-testing-1.jpgAnti-Caspase 3 Rabbit Monoclonal Antibody Western blot analysis of Caspase 3 using anti-Caspase 3 antibody (M00334-9). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human 293T whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 3 antigen affinity purified monoclonal antibody (Catalog # M00334-9) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 3 at approximately 32 kDa. The expected band size for Caspase 3 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-9-wb.jpgAnti-Caspase 3 Rabbit Monoclonal AntibodyWestern blot analysis of Caspase 3 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate; (3) Rat brain lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-9-medscimonit-25-4907-g003.jpgAnti-Caspase 3 Rabbit Monoclonal AntibodyImmunohistochemistry assay for the expression levels and IOD values of COL-II, ADAMTS-4, MMP-13, caspase-3 and Aggrecan in different groups. * P<0.001 vs. control group; # P<0.001 vs. OVX+ALN group. Bars=100 um. Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6618338/'>31265447</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-9-41598_2018_34156_fig5_html.pngAnti-Caspase 3 Rabbit Monoclonal AntibodyProtective effects of maltol on cisplatin-induced injury in HEK293 cells. ( A ) The cytotoxic effects of cisplatin on HEK293 cells. ( B ) Effect of maltol on the activity of normal cells. ( C ) The viability of HEK293 cells incubated with maltol after cisplatin exposure. Effects of maltol on the protein expression levels of Bcl-2, Bax and caspase 3, 8, 9 as well as GAPDH protein was used as a loading control. ( D ) Cells were used for western blot analysis of indicated proteins (upper panel). Column chart represents relative protein levels compared with the control group after normalization to GAPDH levels (lower panel) Values are expressed as mean ± S.D. n = 8. ** p < 0.01 vs . normal group; # p < 0.05, ## p < 0.01 vs . cisplatin group. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-34156-6'>30374107</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-9-medscimonit-24-2849-g006.jpgAnti-Caspase 3 Rabbit Monoclonal AntibodyImmunohistochemical assay for ADAMTS-4, aggrecan, caspase-3, and MMP-13 for every group. Data are represented as mean ±SEM. P values were determined by Fisher’s LSD test following one-way ANOVA. * P <0.05; ** P <0.01; *** P <0.001. Bars=100 μm. Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960220/'>29748528</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-9-casp3-primary-antibodies-ihc-testing-2.jpgAnti-Caspase 3 Rabbit Monoclonal Antibody IHC analysis of Caspase 3 using anti-Caspase 3 antibody (M00334-9). Caspase 3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Caspase 3 Antibody (M00334-9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-9-casp3-primary-antibodies-ihc-testing-3.jpgAnti-Caspase 3 Rabbit Monoclonal Antibody IHC analysis of Caspase 3 using anti-Caspase 3 antibody (M00334-9). Caspase 3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Caspase 3 Antibody (M00334-9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-socs7-rabbit-monoclonal-antibody-m06055-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06055-socs7-primary-antibodies-wb-testing-1.jpgAnti-SOCS7 Rabbit Monoclonal Antibody Western blot analysis of SOCS7 using anti-SOCS7 antibody (M06055). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS7 antigen affinity purified monoclonal antibody (M06055) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS7 at approximately 63 kDa. The expected band size for SOCS7 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbr1-rabbit-monoclonal-antibody-m02825-3-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-wb-testing-1.jpgAnti-CBR1 Rabbit Monoclonal AntibodyWestern blot analysis of CBR1 using anti-CBR1 antibody (M02825-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBR1 antigen affinity purified monoclonal antibody (M02825-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CBR1 at approximately 30 kDa. The expected band size for CBR1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-8.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-9.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-6.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-7.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-5.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-4.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-3.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02825-3-cbr1-primary-antibodies-ihc-testing-2.jpgAnti-CBR1 Rabbit Monoclonal AntibodyIHC analysis of CBR1 using anti-CBR1 antibody (M02825-3). CBR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 Antibody (M02825-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-aanat-picoband-trade-antibody-a03784-1-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03784-1-annat-primary-antibodies-wb-testing-1.jpgAnti-Aanat Antibody Picoband® Western blot analysis of Aanat using anti-Aanat antibody (A03784-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aanat antigen affinity purified polyclonal antibody (Catalog # A03784-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aanat at approximately 23 kDa. The expected band size for Aanat is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-p-glycoprotein-abcb1-picoband-trade-antibody-a00049-5-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-5-abcb1-primary-antibodies-wb-testing-1.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® Western blot analysis of P Glycoprotein/ABCB1 using anti-P Glycoprotein/ABCB1 antibody (A00049-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human DLD1 whole cell lysates, Lane 2: human HUH-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P Glycoprotein/ABCB1 antigen affinity purified polyclonal antibody (Catalog # A00049-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P Glycoprotein/ABCB1 at approximately 160 kDa. The expected band size for P Glycoprotein/ABCB1 is at 140 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-5-abcb1-primary-antibodies-ihc-testing-2.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® IHC analysis of P Glycoprotein/ABCB1 using anti-P Glycoprotein/ABCB1 antibody (A00049-5). P Glycoprotein/ABCB1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P Glycoprotein/ABCB1 Antibody (A00049-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-5-abcb1-primary-antibodies-ihc-testing-3.jpgAnti-P Glycoprotein/ABCB1 Antibody Picoband® IHC analysis of P Glycoprotein/ABCB1 using anti-P Glycoprotein/ABCB1 antibody (A00049-5). P Glycoprotein/ABCB1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P Glycoprotein/ABCB1 Antibody (A00049-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-5-abcb1-primary-antibodies-fcm-testing-4.pngAnti-P Glycoprotein/ABCB1 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-P Glycoprotein/ABCB1 antibody (A00049-5). Overlay histogram showing Caco-2 cells stained with A00049-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P Glycoprotein/ABCB1 Antibody (A00049-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ago1-picoband-trade-antibody-a00826-4-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00826-4-ago1-primary-antibodies-wb-testing-1.jpgAnti-AGO1 Antibody Picoband® Western blot analysis of AGO1 using anti-AGO1 antibody (A00826-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO1 antigen affinity purified polyclonal antibody (Catalog # A00826-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGO1 at approximately 97 kDa. The expected band size for AGO1 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00826-4-ago1-primary-antibodies-fcm-testing-2.pngAnti-AGO1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-AGO1 antibody (A00826-4). Overlay histogram showing U87 cells stained with A00826-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGO1 Antibody (A00826-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ago1-picoband-trade-antibody-a00826-5-boster.html2026-03-17T05:13:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00826-5-ago1-primary-antibodies-wb-testing-1.jpgAnti-AGO1 Antibody Picoband® Western blot analysis of AGO1 using anti-AGO1 antibody (A00826-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO1 antigen affinity purified polyclonal antibody (Catalog # A00826-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGO1 at approximately 97 kDa. The expected band size for AGO1 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00826-5-ago1-primary-antibodies-if-testing-2.jpgAnti-AGO1 Antibody Picoband® IF analysis of AGO1 using anti-AGO1 antibody (A00826-5). AGO1 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AGO1 Antibody (A00826-5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00826-5-ago1-primary-antibodies-fcm-testing-3.pngAnti-AGO1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-AGO1 antibody (A00826-5). Overlay histogram showing MCF-7 cells stained with A00826-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGO1 Antibody (A00826-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aim2-picoband-trade-antibody-a00443-1-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00443-1-aim2-primary-antibodies-wb-testing-1.jpgAnti-Aim2 Antibody Picoband® Western blot analysis of Aim2 using anti-Aim2 antibody (A00443-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aim2 antigen affinity purified polyclonal antibody (Catalog # A00443-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aim2 at approximately 39 kDa. The expected band size for Aim2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00443-1-aim2-primary-antibodies-fcm-testing-2.pngAnti-Aim2 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-Aim2 antibody (A00443-1). Overlay histogram showing RAW264.7 cells stained with A00443-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aim2 Antibody (A00443-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-akt3-picoband-trade-antibody-a00520-2-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00520-2-akt3-primary-antibodies-wb-testing-1_1.jpgAnti-AKT3 Antibody Picoband®Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (A00520-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 56 kDa. The expected band size for AKT3 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00520-2-akt3-primary-antibodies-if-testing-1.jpgAnti-AKT3 Antibody Picoband®IF analysis of AKT3 using anti-AKT3 antibody (PA2260). AKT3 was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AKT3 Antibody (PA2260) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-aldh3a1-picoband-trade-antibody-a01121-5-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01121-5-aldh3a1-primary-antibodies-wb-testing-1.jpgAnti-ALDH3A1 Antibody Picoband® Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # A01121-5) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 50 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01121-5-aldh3a1-primary-antibodies-ihc-testing-2.jpgAnti-ALDH3A1 Antibody Picoband® IHC analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-5). ALDH3A1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH3A1 Antibody (A01121-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01121-5-aldh3a1-primary-antibodies-fcm-testing-3.pngAnti-ALDH3A1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ALDH3A1 antibody (A01121-5). Overlay histogram showing RT4 cells stained with A01121-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH3A1 Antibody (A01121-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-annexin-4-anxa4-picoband-trade-antibody-a04840-2-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-wb-testing-1.jpgAnti-Annexin-4/ANXA4 Antibody Picoband® Western blot analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin-4/ANXA4 antigen affinity purified polyclonal antibody (Catalog # A04840-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin-4/ANXA4 at approximately 36 kDa. The expected band size for Annexin-4/ANXA4 is at 36 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-ihc-testing-2.jpgAnti-Annexin-4/ANXA4 Antibody Picoband® IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Annexin-4/ANXA4 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin-4/ANXA4 Antibody (A04840-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-ihc-testing-3.jpgAnti-Annexin-4/ANXA4 Antibody Picoband® IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Annexin-4/ANXA4 was detected in a paraffin-embedded section of human leiomyoma of the vulva tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin-4/ANXA4 Antibody (A04840-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-ihc-testing-4.jpgAnti-Annexin-4/ANXA4 Antibody Picoband® IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Annexin-4/ANXA4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin-4/ANXA4 Antibody (A04840-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-ihc-testing-5.jpgAnti-Annexin-4/ANXA4 Antibody Picoband® IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Annexin-4/ANXA4 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin-4/ANXA4 Antibody (A04840-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-ihc-testing-6.jpgAnti-Annexin-4/ANXA4 Antibody Picoband® IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Annexin-4/ANXA4 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin-4/ANXA4 Antibody (A04840-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-ihc-testing-7.jpgAnti-Annexin-4/ANXA4 Antibody Picoband® IHC analysis of Annexin-4/ANXA4 using anti-Annexin-4/ANXA4 antibody (A04840-2). Annexin-4/ANXA4 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin-4/ANXA4 Antibody (A04840-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04840-2-anxa4-primary-antibodies-fcm-testing-8.pngAnti-Annexin-4/ANXA4 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-Annexin-4/ANXA4 antibody (A04840-2). Overlay histogram showing HEL cells stained with A04840-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin-4/ANXA4 Antibody (A04840-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tem8-atr-antxr1-picoband-trade-antibody-a01563-2-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01563-2-antxr1-primary-antibodies-wb-testing-1.jpgAnti-TEM8/ATR/ANTXR1 Antibody Picoband® Western blot analysis of TEM8/ATR/ANTXR1 using anti-TEM8/ATR/ANTXR1 antibody (A01563-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEM8/ATR/ANTXR1 antigen affinity purified polyclonal antibody (Catalog # A01563-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEM8/ATR/ANTXR1 at approximately 80 kDa. The expected band size for TEM8/ATR/ANTXR1 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-abp1-aoc1-picoband-trade-antibody-a08386-1-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08386-1-aoc1-primary-antibodies-wb-testing-1.jpgAnti-ABP1/AOC1 Antibody Picoband® Western blot analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (A08386-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HK-2 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABP1/AOC1 antigen affinity purified polyclonal antibody (Catalog # A08386-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABP1/AOC1 at approximately 90 kDa. The expected band size for ABP1/AOC1 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08386-1-aoc1-primary-antibodies-ihc-testing-2.jpgAnti-ABP1/AOC1 Antibody Picoband® IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (A08386-1). ABP1/AOC1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABP1/AOC1 Antibody (A08386-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08386-1-aoc1-primary-antibodies-if-testing-3.jpgAnti-ABP1/AOC1 Antibody Picoband® IF analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (A08386-1). ABP1/AOC1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABP1/AOC1 Antibody (A08386-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08386-1-aoc1-primary-antibodies-fcm-testing-4.pngAnti-ABP1/AOC1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-ABP1/AOC1 antibody (A08386-1). Overlay histogram showing THP-1 cells stained with A08386-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ABP1/AOC1 Antibody (A08386-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-larg-arhgef12-picoband-trade-antibody-a06802-2-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06802-2-arhgef12-primary-antibodies-wb-testing-1.jpgAnti-LARG/ARHGEF12 Antibody Picoband® Western blot analysis of LARG/ARHGEF12 using anti-LARG/ARHGEF12 antibody (A06802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARG/ARHGEF12 antigen affinity purified polyclonal antibody (Catalog # A06802-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The si2093gnal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARG/ARHGEF12 at approximately 220 kDa. The expected band size for LARG/ARHGEF12 is at 173 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06802-2-arhgef12-primary-antibodies-fcm-testing-2.pngAnti-LARG/ARHGEF12 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-LARG/ARHGEF12 antibody (A06802-2). Overlay histogram showing HL-60 cells stained with A06802-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LARG/ARHGEF12 Antibody (A06802-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atp7b-picoband-trade-antibody-a00686-2-boster.html2026-04-06T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00686-2-atp7b-primary-antibodies-wb-testing-1.jpgAnti-ATP7B Antibody Picoband® Western blot analysis of ATP7B using anti-ATP7B antibody (A00686-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP7B antigen affinity purified polyclonal antibody (Catalog # A00686-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP7B at approximately 157 kDa. The expected band size for ATP7B is at 157 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00686-2-atp7b-primary-antibodies-if-testing-2.jpgAnti-ATP7B Antibody Picoband® IF analysis of ATP7B using anti-ATP7B antibody (A00686-2). ATP7B was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATP7B Antibody (A00686-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-sca2-atxn2-picoband-trade-antibody-a01915-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01915-atxn2-primary-antibodies-wb-testing-1_1.jpgAnti-SCA2/ATXN2 Antibody Picoband® Western blot analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCA2/ATXN2 antigen affinity purified polyclonal antibody (Catalog # A01915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCA2/ATXN2 at approximately 140-150 kDa. The expected band size for SCA2/ATXN2 is at 140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01915-atxn2-primary-antibodies-ihc-testing-2.jpgAnti-SCA2/ATXN2 Antibody Picoband® IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915). SCA2/ATXN2 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01915-atxn2-primary-antibodies-ihc-testing-3.jpgAnti-SCA2/ATXN2 Antibody Picoband® IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915). SCA2/ATXN2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01915-atxn2-primary-antibodies-ihc-testing-4.jpgAnti-SCA2/ATXN2 Antibody Picoband® IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915). SCA2/ATXN2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01915-atxn2-primary-antibodies-ihc-testing-5.jpgAnti-SCA2/ATXN2 Antibody Picoband® IHC analysis of SCA2/ATXN2 using anti-SCA2/ATXN2 antibody (A01915). SCA2/ATXN2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCA2/ATXN2 Antibody (A01915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ctip2-bcl11b-picoband-trade-antibody-a01485-1-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-1-bcl11b-primary-antibodies-wb-testing-1.jpgAnti-Ctip2/BCL11B Antibody Picoband® Western blot analysis of Ctip2/BCL11B using anti-Ctip2/BCL11B antibody (A01485-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ctip2/BCL11B antigen affinity purified polyclonal antibody (Catalog # A01485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ctip2/BCL11B at approximately 130 kDa. The expected band size for Ctip2/BCL11B is at 96 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ccl3-picoband-trade-antibody-a00405-3-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00405-3-ccl3-primary-antibodies-ihc-testing-1.jpgAnti-Ccl3 Antibody IHC analysis of Ccl3 using anti-Ccl3 antibody (A00405-3). Ccl3 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl3 Antibody (A00405-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00405-3-ccl3-primary-antibodies-ihc-testing-2.jpgAnti-Ccl3 Antibody IHC analysis of Ccl3 using anti-Ccl3 antibody (A00405-3). Ccl3 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl3 Antibody (A00405-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ccl14-picoband-trade-antibody-a07151-1-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07151-1-ccl14-primary-antibodies-ihc-testing-1.jpgAnti-CCL14 Antibody IHC analysis of CCL14 using anti-CCL14 antibody (A07151-1). CCL14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCL14 Antibody (A07151-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07151-1-ccl14-primary-antibodies-ihc-testing-2.jpgAnti-CCL14 Antibody IHC analysis of CCL14 using anti-CCL14 antibody (A07151-1). CCL14 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCL14 Antibody (A07151-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cd1b-picoband-trade-antibody-a02158-2-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02158-2-cd1b-primary-antibodies-wb-testing-1.jpgAnti-CD1B Antibody Picoband® Western blot analysis of CD1B using anti-CD1B antibody (A02158-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human MOLT-4 tissue lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1B antigen affinity purified polyclonal antibody (Catalog # A02158-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1B at approximately 40 kDa. The expected band size for CD1B is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02158-2-fphys-09-00941-g006.jpgAnti-CD1B Antibody Picoband®(A–F) Cisatracurium inhibits metastatic ability of CRC in vivo . (A) Line graph of subcutaneous tumor volume. (B) Weight of subcutaneous tumors in grams. Data are expressed as mean tumor volume or weight ± SE. ∗ p < 0.05. (C–F) Representative densities of tumor viability and migration regulatory proteins (p53, p21 and CD1, SNAI-1, CALD1, E-Cadherin) in tumor tissue samples. β-Actin was used as internal control. The cluster bar chats in (D , F) indicates the levels of viability and migration regulatory proteins in the treatment group. Data are expressed as Mean ± SEM ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus control. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2018.00941/full'>30108509</a> https://www.bosterbio.com/products/primary-antibodies/anti-cd28-picoband-trade-antibody-a00065-3-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00065-3-cd28-primary-antibodies-wb-testing-1.jpgAnti-CD28 Antibody Picoband® Western blot analysis of CD28 using anti-CD28 antibody (A00065-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HUT-78 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD28 antigen affinity purified polyclonal antibody (Catalog # A00065-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD28 at approximately 60 kDa. The expected band size for CD28 is at 25 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00065-3-cd28-primary-antibodies-fcm-testing-2.pngAnti-CD28 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-CD28 antibody (A00065-3). Overlay histogram showing HEL cells stained with A00065-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD28 Antibody (A00065-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cdc25b-picoband-trade-antibody-a01899-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01899-cdc25b-primary-antibodies-wb-testing-1.jpgAnti-Cdc25b Antibody Picoband® Western blot analysis of Cdc25b using anti-Cdc25b antibody (A01899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25b antigen affinity purified polyclonal antibody (Catalog # A01899) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25b at approximately 65 kDa. The expected band size for Cdc25b is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01899-cdc25b-primary-antibodies-ihc-testing-2.jpgAnti-Cdc25b Antibody Picoband® IHC analysis of Cdc25b using anti-Cdc25b antibody (A01899). Cdc25b was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cdc25b Antibody (A01899) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cux1-picoband-trade-antibody-a01653-4-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01653-4-cux1-primary-antibodies-wb-testing-1.jpgAnti-CUX1 Antibody Picoband® Western blot analysis of CUX1 using anti-CUX1 antibody (A01653-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUX1 antigen affinity purified polyclonal antibody (Catalog # A01653-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CUX1 at approximately 200 kDa. The expected band size for CUX1 is at 164 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01653-4-cux1-primary-antibodies-ihc-testing-2.jpgAnti-CUX1 Antibody Picoband® IHC analysis of CUX1 using anti-CUX1 antibody (A01653-4). CUX1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CUX1 Antibody (A01653-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01653-4-cux1-primary-antibodies-ihc-testing-3.jpgAnti-CUX1 Antibody Picoband® IHC analysis of CUX1 using anti-CUX1 antibody (A01653-4). CUX1 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CUX1 Antibody (A01653-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01653-4-cux1-primary-antibodies-ihc-testing-4.jpgAnti-CUX1 Antibody Picoband® IHC analysis of CUX1 using anti-CUX1 antibody (A01653-4). CUX1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CUX1 Antibody (A01653-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01653-4-cux1-primary-antibodies-ihc-testing-5.jpgAnti-CUX1 Antibody Picoband® IHC analysis of CUX1 using anti-CUX1 antibody (A01653-4). CUX1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CUX1 Antibody (A01653-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01653-4-cux1-primary-antibodies-fcm-testing-6.pngAnti-CUX1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CUX1 antibody (A01653-4). Overlay histogram showing HepG2 cells stained with A01653-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CUX1 Antibody (A01653-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cxcr3-picoband-trade-antibody-a00295-2-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00295-2-cxcr3-primary-antibodies-wb-testing-1.jpgAnti-Cxcr3 Antibody Picoband® Western blot analysis of Cxcr3 using anti-Cxcr3 antibody (A00295-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cxcr3 antigen affinity purified polyclonal antibody (Catalog # A00295-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cxcr3 at approximately 46 kDa. The expected band size for Cxcr3 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00295-2-cxcr3-primary-antibodies-ihc-testing-2.jpgAnti-Cxcr3 Antibody Picoband® IHC analysis of Cxcr3 using anti-Cxcr3 antibody (A00295-2). Cxcr3 was detected in a paraffin-embedded section of mouse intestinal lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cxcr3 Antibody (A00295-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00295-2-cxcr3-primary-antibodies-ihc-testing-3.jpgAnti-Cxcr3 Antibody Picoband® IHC analysis of Cxcr3 using anti-Cxcr3 antibody (A00295-2). Cxcr3 was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cxcr3 Antibody (A00295-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cxcr5-picoband-trade-antibody-a00663-1-boster.html2026-03-17T05:13:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00663-1-cxcr5-primary-antibodies-ihc-testing-1.jpgAnti-Cxcr5 Antibody IHC analysis of TFEB using anti-TFEB antibody (A00663-1). TFEB was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00663-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00663-1-cxcr5-primary-antibodies-ihc-testing-2.jpgAnti-Cxcr5 Antibody IHC analysis of TFEB using anti-TFEB antibody (A00663-1). TFEB was detected in a paraffin-embedded section of rat lymph nodes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00663-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-dao-picoband-trade-antibody-a00773-4-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00773-4-dao-primary-antibodies-wb-testing-1.jpgAnti-DAO Antibody Picoband® Western blot analysis of DAO using anti-DAO antibody (A00773-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAO antigen affinity purified polyclonal antibody (Catalog # A00773-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAO at approximately 36 kDa. The expected band size for DAO is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00773-4-dao-primary-antibodies-fcm-testing-2.pngAnti-DAO Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-DAO antibody (A00773-4). Overlay histogram showing HL-60 cells stained with A00773-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAO Antibody (A00773-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dkk1-picoband-trade-antibody-a00632-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00632-3-dkk1-primary-antibodies-ihc-testing-1.jpgAnti-Dkk1 Antibody IHC analysis of Dkk1 using anti-Dkk1 antibody (A00632-3). Dkk1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dkk1 Antibody (A00632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00632-3-dkk1-primary-antibodies-ihc-testing-2.jpgAnti-Dkk1 Antibody IHC analysis of Dkk1 using anti-Dkk1 antibody (A00632-3). Dkk1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dkk1 Antibody (A00632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00632-3-dkk1-primary-antibodies-ihc-testing-3.jpgAnti-Dkk1 Antibody IHC analysis of Dkk1 using anti-Dkk1 antibody (A00632-3). Dkk1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dkk1 Antibody (A00632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-drp1-dnm1l-picoband-trade-antibody-a00556-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00556-3-dnm1l-primary-antibodies-wb-testing-1.jpgAnti-DRP1/DNM1L Antibody Picoband® Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody (A00556-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody (Catalog # A00556-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 75 kDa. The expected band size for DRP1/DNM1L is at 82 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-her2-erbb2-picoband-trade-antibody-a00010-4-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00010-4-erbb2-primary-antibodies-wb-testing-1.jpgAnti-HER2/ERBB2 Antibody Picoband® Western blot analysis of HER2/ERBB2 using anti-HER2/ERBB2 antibody (A00010-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HER2/ERBB2 antigen affinity purified polyclonal antibody (Catalog # A00010-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HER2/ERBB2 at approximately 185 kDa. The expected band size for HER2/ERBB2 is at 138 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00010-4-erbb2-primary-antibodies-ihc-testing-2.jpgAnti-HER2/ERBB2 Antibody Picoband® IHC analysis of HER2/ERBB2 using anti-HER2/ERBB2 antibody (A00010-4). HER2/ERBB2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER2/ERBB2 Antibody (A00010-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ercc1-picoband-trade-antibody-a00388-4-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00388-4-ercc1-primary-antibodies-wb-testing-1.jpgAnti-ERCC1 Antibody Picoband® Western blot analysis of ERCC1 using anti-ERCC1 antibody (A00388-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 antigen affinity purified polyclonal antibody (Catalog # A00388-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERCC1 at approximately 38 kDa. The expected band size for ERCC1 is at 33 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00388-4-ercc1-primary-antibodies-fcm-testing-2.pngAnti-ERCC1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ERCC1 antibody (A00388-4). Overlay histogram showing RT4 cells stained with A00388-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERCC1 Antibody (A00388-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-jam-a-f11r-picoband-trade-antibody-a02068-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02068-3-f11r-primary-antibodies-wb-testing-1_1.jpgAnti-JAM-A/F11R Antibody Picoband® Western blot analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (A02068-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAM-A/F11R antigen affinity purified polyclonal antibody (A02068-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for JAM-A/F11R at approximately 38-40 kDa. The expected band size for JAM-A/F11R is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02068-3-f11r-primary-antibodies-ihc-testing-2_1.jpgAnti-JAM-A/F11R Antibody Picoband® IHC analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (A02068-3). JAM-A/F11R was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAM-A/F11R Antibody (A02068-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02068-3-f11r-primary-antibodies-ihc-testing-3_1.jpgAnti-JAM-A/F11R Antibody Picoband® IHC analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (A02068-3). JAM-A/F11R was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAM-A/F11R Antibody (A02068-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02068-3-f11r-primary-antibodies-fcm-testing-4.jpgAnti-JAM-A/F11R Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-JAM-A/F11R antibody (A02068-3). Overlay histogram showing MCF-7 cells stained with A02068-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-JAM-A/F11R Antibody (A02068-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02068-3-f11r-primary-antibodies-fcm-testing-5.jpgAnti-JAM-A/F11R Antibody Picoband® Flow Cytometry analysis of A2780 cells using anti-JAM-A/F11R antibody (A02068-3). Overlay histogram showing A2780 cells stained with A02068-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAM-A/F11R Antibody (A02068-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fer-picoband-trade-antibody-a01630-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01630-3-fer-primary-antibodies-wb-testing-1.jpgAnti-FER Antibody Picoband® Western blot analysis of FER using anti-FER antibody (A01630-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FER antigen affinity purified polyclonal antibody (Catalog # A01630-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FER at approximately 95 kDa. The expected band size for FER is at 95 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-fes-picoband-trade-antibody-a01453-2-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01453-2-fes-primary-antibodies-wb-testing-1.jpgAnti-FES Antibody Picoband® Western blot analysis of FES using anti-FES antibody (A01453-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FES antigen affinity purified polyclonal antibody (Catalog # A01453-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FES at approximately 93 kDa. The expected band size for FES is at 93 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-grk3-picoband-trade-antibody-a32254-1-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32254-1-grk3-primary-antibodies-wb-testing-1.jpgAnti-GRK3 Antibody Picoband® Western blot analysis of GRK3 using anti-GRK3 antibody (A32254-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK3 antigen affinity purified polyclonal antibody (Catalog # A32254-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRK3 at approximately 80 kDa. The expected band size for GRK3 is at 80 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-il2ra-picoband-trade-antibody-a00214-2-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00214-2-il2ra-primary-antibodies-ihc-testing-1.jpgAnti-Il2ra Antibody IHC analysis of Il2ra using anti-Il2ra antibody (A00214-2). Il2ra was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Il2ra Antibody (A00214-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00214-2-il2ra-primary-antibodies-ihc-testing-2.jpgAnti-Il2ra Antibody IHC analysis of Il2ra using anti-Il2ra antibody (A00214-2). Il2ra was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Il2ra Antibody (A00214-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00214-2-il2ra-primary-antibodies-if-testing-3.jpgAnti-Il2ra Antibody IF analysis of Il2ra using anti-Il2ra antibody (A00214-2). Il2ra was detected in an immunocytochemical section of RAW264.7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Il2ra Antibody (A00214-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-il34-picoband-trade-antibody-a06903-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06903-3-il34-primary-antibodies-wb-testing-1.jpgAnti-IL34 Antibody Picoband® Western blot analysis of IL34 using anti-IL34 antibody (A06903-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL34 antigen affinity purified polyclonal antibody (Catalog # A06903-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL34 at approximately 45 kDa. The expected band size for IL34 is at 27 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06903-3-il34-primary-antibodies-ihc-testing-2.jpgAnti-IL34 Antibody Picoband® IHC analysis of IL34 using anti-IL34 antibody (A06903-3). IL34 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL34 Antibody (A06903-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cd103-itgae-picoband-trade-antibody-a07091-2-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07091-2-itgae-primary-antibodies-ihc-testing-1.jpgAnti-CD103/Itgae Antibody IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (A07091-2). CD103/Itgae was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD103/Itgae Antibody (A07091-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07091-2-itgae-primary-antibodies-ihc-testing-2.jpgAnti-CD103/Itgae Antibody IHC analysis of CD103/Itgae using anti-CD103/Itgae antibody (A07091-2). CD103/Itgae was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD103/Itgae Antibody (A07091-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-tip60-kat5-picoband-trade-antibody-a01393-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01393-3-kat5-primary-antibodies-wb-testing-1_1.jpgAnti-Tip60/KAT5 Antibody Picoband®Western blot analysis of Tip60/KAT5 using anti-Tip60/KAT5 antibody (A01393-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tip60/KAT5 antigen affinity purified polyclonal antibody (A01393-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Tip60/KAT5 at approximately 59 kDa. The expected band size for Tip60/KAT5 is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-kcnd1-picoband-trade-antibody-a11599-1-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11599-1-kcnd1-primary-antibodies-wb-testing-1.jpgAnti-KCND1 Antibody Picoband® Western blot analysis of KCND1 using anti-KCND1 antibody (A11599-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCND1 antigen affinity purified polyclonal antibody (Catalog # A11599-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCND1 at approximately 71 kDa. The expected band size for KCND1 is at 71 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-h-erg-kcnh2-picoband-trade-antibody-a00781-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00781-3-kcnh2-primary-antibodies-wb-testing-1.jpgAnti-H-ERG/KCNH2 Antibody Picoband® Western blot analysis of H-ERG/KCNH2 using anti-H-ERG/KCNH2 antibody (A00781-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissuelysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H-ERG/KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H-ERG/KCNH2 at approximately 130 kDa. The expected band size for H-ERG/KCNH2 is at 127 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00781-3-kcnh2-primary-antibodies-fcm-testing-2.pngAnti-H-ERG/KCNH2 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-H-ERG/KCNH2 antibody (A00781-3). Overlay histogram showing JK cells stained with A00781-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-H-ERG/KCNH2 Antibody (A00781-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-kcnn4-picoband-trade-antibody-a01936-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01936-3-kcn44-primary-antibodies-wb-testing-1.jpgAnti-KCNN4 Antibody Picoband® Western blot analysis of KCNN4 using anti-KCNN4 antibody (A01936-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # A01936-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 45 kDa. The expected band size for KCNN4 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-jhdm3a-jmjd2a-kdm4a-picoband-trade-antibody-a02850-1-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02850-1-kdm4a-primary-antibodies-wb-testing-1.jpgAnti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® Western blot analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JHDM3A/JMJD2A/KDM4A antigen affinity purified polyclonal antibody (Catalog # A02850-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JHDM3A/JMJD2A/KDM4A at approximately 150 kDa. The expected band size for JHDM3A/JMJD2A/KDM4A is at 121 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02850-1-kdm4a-primary-antibodies-ihc-testing-2.jpgAnti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1). JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human ovarian serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02850-1-kdm4a-primary-antibodies-ihc-testing-3.jpgAnti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1). JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02850-1-kdm4a-primary-antibodies-ihc-testing-4.jpgAnti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1). JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human aryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02850-1-kdm4a-primary-antibodies-ihc-testing-5.jpgAnti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1). JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02850-1-kdm4a-primary-antibodies-fcm-testing-6.pngAnti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-JHDM3A/JMJD2A/KDM4A antibody (A02850-1). Overlay histogram showing HepG2 cells stained with A02850-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (A02850-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-l1cam-picoband-trade-antibody-a00729-4-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00729-4-l1cam-primary-antibodies-wb-testing-1_1.jpgAnti-L1CAM Antibody Picoband® Western blot analysis of L1CAM using anti-L1CAM antibody (A00729-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L1CAM antigen affinity purified polyclonal antibody (Catalog # A00729-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for L1CAM at approximately 220 kDa. The expected band size for L1CAM is at 140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00729-4-l1cam-primary-antibodies-fcm-testing-2.pngAnti-L1CAM Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-L1CAM antibody (A00729-4). Overlay histogram showing Caco-2 cells stained with A00729-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-L1CAM Antibody (A00729-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-lamp2-picoband-trade-antibody-a01573-3-boster.html2026-03-17T05:13:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01573-3-lamp2-primary-antibodies-wb-testing-1.jpgAnti-LAMP2 Antibody Picoband® Western blot analysis of LAMP2 using anti-LAMP2 antibody (A01573-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP2 antigen affinity purified polyclonal antibody (Catalog # A01573-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP2 at approximately 100-110 kDa. The expected band size for LAMP2 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-lhx4-picoband-trade-antibody-a05503-1-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05503-1-lhx4-primary-antibodies-wb-testing-1.jpgAnti-LHX4 Antibody Picoband® Western blot analysis of LHX4 using anti-LHX4 antibody (A05503-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHX4 antigen affinity purified polyclonal antibody (Catalog # A05503-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LHX4 at approximately 43 kDa. The expected band size for LHX4 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05503-1-lhx4-primary-antibodies-fcm-testing-2.pngAnti-LHX4 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-LHX4 antibody (A05503-1). Overlay histogram showing RT4 cells stained with A05503-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LHX4 Antibody (A05503-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mkk6-map2k6-picoband-trade-antibody-a02011-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02011-3-map2k6-primary-antibodies-wb-testing-1.jpgAnti-MKK6/MAP2K6 Antibody Picoband® Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-erk5-mapk7-picoband-trade-antibody-a02812-4-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02812-4-mapk7-primary-antibodies-wb-testing-1.jpgAnti-ERK5/MAPK7 Antibody Picoband® Western blot analysis of ERK5/MAPK7 using anti-ERK5/MAPK7 antibody (A02812-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERK5/MAPK7 antigen affinity purified polyclonal antibody (Catalog # A02812-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERK5/MAPK7 at approximately 115 kDa. The expected band size for ERK5/MAPK7 is at 88 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-muc1-picoband-trade-antibody-a00187-4-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00187-4-muc1-primary-antibodies-ihc-testing-1.jpgAnti-MUC1 Antibody IHC analysis of MUC1 using anti-MUC1 antibody (A00187-4). MUC1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC1 Antibody (A00187-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00187-4-muc1-primary-antibodies-ihc-testing-2.jpgAnti-MUC1 Antibody IHC analysis of MUC1 using anti-MUC1 antibody (A00187-4). MUC1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC1 Antibody (A00187-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00187-4-muc1-primary-antibodies-ihc-testing-3.jpgAnti-MUC1 Antibody IHC analysis of MUC1 using anti-MUC1 antibody (A00187-4). MUC1 was detected in a paraffin-embedded section of human ovarian carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC1 Antibody (A00187-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-muc2-picoband-trade-antibody-a01212-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01212-3-muc2-primary-antibodies-ihc-testing-1.jpgAnti-MUC2 Antibody IHC analysis of MUC2 using anti-MUC2 antibody (A01212-3). MUC2 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01212-3-muc2-primary-antibodies-ihc-testing-2.jpgAnti-MUC2 Antibody IHC analysis of MUC2 using anti-MUC2 antibody (A01212-3). MUC2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01212-3-muc2-primary-antibodies-ihc-testing-3.jpgAnti-MUC2 Antibody IHC analysis of MUC2 using anti-MUC2 antibody (A01212-3). MUC2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01212-3-muc2-primary-antibodies-ihc-testing-4.jpgAnti-MUC2 Antibody IHC analysis of MUC2 using anti-MUC2 antibody (A01212-3). MUC2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MUC2 Antibody (A01212-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-neurocan-ncan-picoband-trade-antibody-a06700-2-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06700-2-ncan-primary-antibodies-wb-testing-1.jpgAnti-Neurocan/NCAN Antibody Picoband® Western blot analysis of Neurocan/NCAN using anti-Neurocan/NCAN antibody (A06700-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan/NCAN antigen affinity purified polyclonal antibody (Catalog # A06700-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurocan/NCAN at approximately 142 kDa. The expected band size for Neurocan/NCAN is at 136 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ncr1-picoband-trade-antibody-a01516-2-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01516-2-ncr1-primary-antibodies-ihc-testing-1.jpgAnti-NCR1 Antibody IHC analysis of NCR1 using anti-NCR1 antibody (A01516-2). NCR1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCR1 Antibody (A01516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01516-2-ncr1-primary-antibodies-ihc-testing-2.jpgAnti-NCR1 Antibody IHC analysis of NCR1 using anti-NCR1 antibody (A01516-2). NCR1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCR1 Antibody (A01516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01516-2-ncr1-primary-antibodies-fc-testing-1.jpgAnti-NCR1 AntibodyFlow Cytometry analysis of mouse spleen cells using anti-NCR1 antibody (A01516-2). Overlay histogram showing mouse spleen cells stained with A01516-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NCR1 Antibody (A01516-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank contro https://www.bosterbio.com/products/primary-antibodies/anti-nms-picoband-trade-antibody-a15072-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15072-3-nms-primary-antibodies-ihc-testing-1.jpgAnti-Nms Antibody IHC analysis of Nms using anti-Nms antibody (A15072-3). Nms was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nms Antibody (A15072-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15072-3-nms-primary-antibodies-ihc-testing-2.jpgAnti-Nms Antibody IHC analysis of Nms using anti-Nms antibody (A15072-3). Nms was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nms Antibody (A15072-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-pkib-picoband-trade-antibody-a12582-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12582-3-pkib-primary-antibodies-ihc-testing-1.jpgAnti-PKIB Antibody IHC analysis of PKIB using anti-PKIB antibody (A12582-3). PKIB was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKIB Antibody (A12582-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-gadd34-ppp1r15a-picoband-trade-antibody-a02394-2-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02394-2-ppp1r15a-primary-antibodies-wb-testing-1.jpgAnti-GADD34/PPP1R15A Antibody Picoband® Western blot analysis of GADD34/PPP1R15A using anti-GADD34/PPP1R15A antibody (A02394-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human SIHA whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/PPP1R15A antigen affinity purified polyclonal antibody (Catalog # A02394-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/PPP1R15A at approximately 100 kDa. The expected band size for GADD34/PPP1R15A is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02394-2-ppp1r15a-primary-antibodies-ihc-testing-2.jpgAnti-GADD34/PPP1R15A Antibody Picoband® IHC analysis of GADD34/PPP1R15A using anti-GADD34/PPP1R15A antibody (A02394-2). GADD34/PPP1R15A was detected in a paraffin-embedded section of human melanom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GADD34/PPP1R15A Antibody (A02394-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-gadd34-ppp1r15a-picoband-trade-antibody-a02394-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02394-3-ppp1r15a-primary-antibodies-wb-testing-1.jpgAnti-GADD34/PPP1R15A Antibody Picoband® Western blot analysis of GADD34/PPP1R15A using anti-GADD34/PPP1R15A antibody (A02394-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/PPP1R15A antigen affinity purified polyclonal antibody (Catalog # A02394-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/PPP1R15A at approximately 100 kDa. The expected band size for GADD34/PPP1R15A is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-prmt5-picoband-trade-antibody-a00635-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-wb-testing-1.jpgAnti-PRMT5 Antibody Picoband® Western blot analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human Daudi whole cell lysates, Lane 9: rat brain tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT5 antigen affinity purified polyclonal antibody (Catalog # A00635-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT5 at approximately 72 kDa. The expected band size for PRMT5 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-ihc-testing-2.jpgAnti-PRMT5 Antibody Picoband® IHC analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). PRMT5 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRMT5 Antibody (A00635-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-ihc-testing-3.jpgAnti-PRMT5 Antibody Picoband® IHC analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). PRMT5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRMT5 Antibody (A00635-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-ihc-testing-4.jpgAnti-PRMT5 Antibody Picoband® IHC analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). PRMT5 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRMT5 Antibody (A00635-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-ihc-testing-5.jpgAnti-PRMT5 Antibody Picoband® IHC analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). PRMT5 was detected in a paraffin-embedded section of human thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRMT5 Antibody (A00635-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-ihc-testing-6.jpgAnti-PRMT5 Antibody Picoband® IHC analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). PRMT5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRMT5 Antibody (A00635-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-ihc-testing-7.jpgAnti-PRMT5 Antibody Picoband® IHC analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). PRMT5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRMT5 Antibody (A00635-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00635-3-prmt5-primary-antibodies-ihc-testing-8.jpgAnti-PRMT5 Antibody Picoband® IHC analysis of PRMT5 using anti-PRMT5 antibody (A00635-3). PRMT5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRMT5 Antibody (A00635-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-rgs12-picoband-trade-antibody-a07317-2-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07317-2-rgs12-primary-antibodies-wb-testing-1.jpgAnti-RGS12 Antibody Picoband® Western blot analysis of RGS12 using anti-RGS12 antibody (A07317-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS12 antigen affinity purified polyclonal antibody (Catalog # A07317-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS12 at approximately 156 kDa. The expected band size for RGS12 is at 156 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07317-2-rgs12-primary-antibodies-ihc-testing-2.jpgAnti-RGS12 Antibody Picoband® IHC analysis of RGS12 using anti-RGS12 antibody (A07317-2). RGS12 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RGS12 Antibody (A07317-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07317-2-rgs12-primary-antibodies-ihc-testing-3.jpgAnti-RGS12 Antibody Picoband® IHC analysis of RGS12 using anti-RGS12 antibody (A07317-2). RGS12 was detected in a paraffin-embedded section of rat brian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RGS12 Antibody (A07317-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07317-2-rgs12-primary-antibodies-if-testing-4.jpgAnti-RGS12 Antibody Picoband® IF analysis of RGS12 using anti-RGS12 antibody (A07317-2). RGS12 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RGS12 Antibody (A07317-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-spt5-supt5h-picoband-trade-antibody-a03426-2-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03426-2-supt5h-primary-antibodies-wb-testing-1.jpgAnti-SPT5/SUPT5H Antibody Picoband® Western blot analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPT5/SUPT5H antigen affinity purified polyclonal antibody (Catalog # A03426-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPT5/SUPT5H at approximately 150 kDa. The expected band size for SPT5/SUPT5H is at 121 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03426-2-supt5h-primary-antibodies-ihc-testing-2.jpgAnti-SPT5/SUPT5H Antibody Picoband® IHC analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). SPT5/SUPT5H was detected in a paraffin-embedded section of human ovarian serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPT5/SUPT5H Antibody (A03426-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03426-2-supt5h-primary-antibodies-ihc-testing-3.jpgAnti-SPT5/SUPT5H Antibody Picoband® IHC analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). SPT5/SUPT5H was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPT5/SUPT5H Antibody (A03426-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03426-2-supt5h-primary-antibodies-ihc-testing-4.jpgAnti-SPT5/SUPT5H Antibody Picoband® IHC analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). SPT5/SUPT5H was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPT5/SUPT5H Antibody (A03426-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03426-2-supt5h-primary-antibodies-if-testing-5.jpgAnti-SPT5/SUPT5H Antibody Picoband® IF analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2) and anti-Tubulin beta antibody (M05613-4). SPT5/SUPT5H was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SPT5/SUPT5H Antibody (A03426-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03426-2-supt5h-primary-antibodies-fcm-testing-6.pngAnti-SPT5/SUPT5H Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-SPT5/SUPT5H antibody (A03426-2). Overlay histogram showing THP-1 cells stained with A03426-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPT5/SUPT5H Antibody (A03426-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tcf4-picoband-trade-antibody-a00674-2-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00674-2-tcf4-primary-antibodies-wb-testing-1.jpgAnti-TCF4 Antibody Picoband® Western blot analysis of TCF4 using anti-TCF4 antibody (A00674-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF4 antigen affinity purified polyclonal antibody (Catalog # A00674-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCF4 at approximately 71 kDa. The expected band size for TCF4 is at 71 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tfeb-picoband-trade-antibody-a00662-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-3-tfeb-primary-antibodies-wb-testing-1.jpgAnti-TFEB Antibody Picoband® Western blot analysis of TFEB using anti-TFEB antibody (A00662-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody (Catalog # A00662-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFEB at approximately 70 kDa. The expected band size for TFEB is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tlr9-picoband-trade-antibody-a00198-3-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00198-3-tlr9-primary-antibodies-wb-testing-1.jpgAnti-TLR9 Antibody Picoband® Western blot analysis of TLR9 using anti-TLR9 antibody (A00198-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR9 antigen affinity purified polyclonal antibody (Catalog # A00198-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR9 at approximately 116 kDa. The expected band size for TLR9 is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00198-3-tlr9-primary-antibodies-ihc-testing-2.jpgAnti-TLR9 Antibody Picoband® IHC analysis of TLR9 using anti-TLR9 antibody (A00198-3). TLR9 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TLR9 Antibody (A00198-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-matriptase-2-tmprss6-picoband-trade-antibody-a02093-1-boster.html2026-03-17T05:13:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02093-1-tmprss6-primary-antibodies-wb-testing-1.jpgAnti-Matriptase 2/TMPRSS6 Antibody Picoband® Western blot analysis of Matriptase 2/TMPRSS6 using anti-Matriptase 2/TMPRSS6 antibody (A02093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Matriptase 2/TMPRSS6 antigen affinity purified polyclonal antibody (Catalog # A02093-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Matriptase 2/TMPRSS6 at approximately 90,95kDa. The expected band size for Matriptase 2/TMPRSS6 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02093-1-tmprss6-primary-antibodies-fcm-testing-2.pngAnti-Matriptase 2/TMPRSS6 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Matriptase 2/TMPRSS6 antibody (A02093-1). Overlay histogram showing MCF-7 cells stained with A02093-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Matriptase 2/TMPRSS6 Antibody (A02093-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trpv3-picoband-trade-antibody-a03874-2-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03874-2-trpv3-primary-antibodies-wb-testing-1.jpgAnti-TRPV3 Antibody Picoband® Western blot analysis of TRPV3 using anti-TRPV3 antibody (A03874-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV3 antigen affinity purified polyclonal antibody (Catalog # A03874-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV3 at approximately 100 kDa. The expected band size for TRPV3 is at 156 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-usp10-picoband-trade-antibody-a03786-3-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03786-3-usp10-primary-antibodies-wb-testing-1.jpgAnti-USP10 Antibody Picoband® Western blot analysis of USP10 using anti-USP10 antibody (A03786-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SIHA whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP10 antigen affinity purified polyclonal antibody (Catalog # A03786-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP10 at approximately 110,130 kDa. The expected band size for USP10 is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-wisp-1-ccn4-picoband-trade-antibody-a03052-1-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03052-1-wisp1-primary-antibodies-wb-testing-1.jpgAnti-WISP-1/CCN4 Antibody Picoband® Western blot analysis of WISP-1/CCN4 using anti-WISP-1/CCN4 antibody (A03052-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WISP-1/CCN4 antigen affinity purified polyclonal antibody (Catalog # A03052-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WISP-1/CCN4 at approximately 48 kDa. The expected band size for WISP-1/CCN4 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ndfip2-picoband-trade-antibody-m08384-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08384-ndfip2-primary-antibodies-wb-testing-1.jpgAnti-NDFIP2 Antibody Picoband® (monoclonal, 10D6D7) Western blot analysis of NDFIP2 using anti-NDFIP2 antibody (M08384). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NDFIP2 antigen affinity purified monoclonal antibody (Catalog # M08384) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NDFIP2 at approximately 39 kDa. The expected band size for NDFIP2 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08384-ndfip2-primary-antibodies-ihc-testing-2.jpgAnti-NDFIP2 Antibody Picoband® (monoclonal, 10D6D7) IHC analysis of NDFIP2 using anti-NDFIP2 antibody (M08384). NDFIP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NDFIP2 Antibody (M08384) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08384-ndfip2-primary-antibodies-ihc-testing-3.jpgAnti-NDFIP2 Antibody Picoband® (monoclonal, 10D6D7) IHC analysis of NDFIP2 using anti-NDFIP2 antibody (M08384). NDFIP2 was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NDFIP2 Antibody (M08384) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08384-ndfip2-primary-antibodies-ihc-testing-4.jpgAnti-NDFIP2 Antibody Picoband® (monoclonal, 10D6D7) IHC analysis of NDFIP2 using anti-NDFIP2 antibody (M08384). NDFIP2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NDFIP2 Antibody (M08384) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08384-ndfip2-primary-antibodies-ihc-testing-5.jpgAnti-NDFIP2 Antibody Picoband® (monoclonal, 10D6D7) IHC analysis of NDFIP2 using anti-NDFIP2 antibody (M08384). NDFIP2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NDFIP2 Antibody (M08384) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08384-ndfip2-primary-antibodies-if-testing-6.jpgAnti-NDFIP2 Antibody Picoband® (monoclonal, 10D6D7) IF analysis of NDFIP2 using anti-NDFIP2 antibody (M08384). NDFIP2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-NDFIP2 Antibody (M08384) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08384-ndfip2-primary-antibodies-fcm-testing-7.jpgAnti-NDFIP2 Antibody Picoband® (monoclonal, 10D6D7) Flow Cytometry analysis of JK cells using anti-NDFIP2 antibody (M08384). Overlay histogram showing JK cells stained with M08384 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NDFIP2 Antibody (M08384, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-alpha-v-itgav-picoband-trade-antibody-m01561-2-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01561-2-itgav-primary-antibodies-wb-testing-1_1.jpgAnti-Integrin alpha V/ITGAV Antibody Picoband® (monoclonal, 8B10H2) Western blot analysis of ITGAV using anti-ITGAV antibody (M01561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human SiHa whole cell lysates, Lane 8: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ITGAV antigen affinity purified monoclonal antibody (Catalog # M01561-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ITGAV at approximately 130 kDa. The expected band size for ITGAV is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01561-2-itgav-primary-antibodies-if-testing-2.jpgAnti-Integrin alpha V/ITGAV Antibody Picoband® (monoclonal, 8B10H2) IF analysis of ITGAV using anti-ITGAV antibody (M01561-2). ITGAV was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-ITGAV Antibody (M01561-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01561-2-itgav-primary-antibodies-ihc-testing-3.jpgAnti-Integrin alpha V/ITGAV Antibody Picoband® (monoclonal, 8B10H2) IHC analysis of ITGAV using anti-ITGAV antibody (M01561-2). ITGAV was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ITGAV Antibody (M01561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01561-2-itgav-primary-antibodies-ihc-testing-4.jpgAnti-Integrin alpha V/ITGAV Antibody Picoband® (monoclonal, 8B10H2) IHC analysis of ITGAV using anti-ITGAV antibody (M01561-2). ITGAV was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ITGAV Antibody (M01561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-band-3-picoband-trade-antibody-m01146-1-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01146-1-band-3-primary-antibodies-wb-testing-1.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband® (monoclonal, 5G2G7) Western blot analysis of SLC4A1 using anti-SLC4A1 antibody (M01146-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SLC4A1 antigen affinity purified monoclonal antibody (Catalog # M01146-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SLC4A1 at approximately 102 kDa. The expected band size for SLC4A1 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01146-1-band-3-primary-antibodies-ihc-testing-2.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband® (monoclonal, 5G2G7) IHC analysis of SLC4A1 using anti-SLC4A1 antibody (M01146-1). SLC4A1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SLC4A1 Antibody (M01146-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01146-1-band-3-primary-antibodies-fcm-testing-3.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband® (monoclonal, 5G2G7) Flow Cytometry analysis of HepG2 cells using anti-SLC4A1 antibody (M01146-1). Overlay histogram showing HepG2 cells stained with M01146-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-SLC4A1 Antibody (M01146-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-drebrin-dbn1-picoband-trade-antibody-m05530-4-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05530-4-dbn1-primary-antibodies-wb-testing-1.jpgAnti-Drebrin/DBN1 Antibody Picoband® (monoclonal, 4F6E7) Western blot analysis of DBN1 using anti-DBN1 antibody (M05530-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Molt4 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DBN1 antigen affinity purified monoclonal antibody (Catalog # M05530-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for DBN1 at approximately 120 kDa. The expected band size for DBN1 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05530-4-dbn1-primary-antibodies-ihc-testing-2.jpgAnti-Drebrin/DBN1 Antibody Picoband® (monoclonal, 4F6E7) IHC analysis of DBN1 using anti-DBN1 antibody (M05530-4). DBN1 was detected in a paraffin-embedded section of human ovarian serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DBN1 Antibody (M05530-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05530-4-dbn1-primary-antibodies-ihc-testing-3.jpgAnti-Drebrin/DBN1 Antibody Picoband® (monoclonal, 4F6E7) IHC analysis of DBN1 using anti-DBN1 antibody (M05530-4). DBN1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DBN1 Antibody (M05530-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05530-4-dbn1-primary-antibodies-ihc-testing-4.jpgAnti-Drebrin/DBN1 Antibody Picoband® (monoclonal, 4F6E7) IHC analysis of DBN1 using anti-DBN1 antibody (M05530-4). DBN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-DBN1 Antibody (M05530-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05530-4-dbn1-primary-antibodies-fcm-testing-5.jpgAnti-Drebrin/DBN1 Antibody Picoband® (monoclonal, 4F6E7) Flow Cytometry analysis of JK cells using anti-DBN1 antibody (M05530-4). Overlay histogram showing JK cells stained with M05530-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-DBN1 Antibody (M05530-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mppb-pmpcb-picoband-trade-antibody-m11793-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11793-pmpcb-primary-antibodies-wb-testing-1.jpgAnti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) Western blot analysis of PMPCB using anti-PMPCB antibody (M11793). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HCCT tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PMPCB antigen affinity purified monoclonal antibody (Catalog # M11793) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PMPCB at approximately 43 kDa. The expected band size for PMPCB is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11793-pmpcb-primary-antibodies-ihc-testing-2.jpgAnti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) IHC analysis of PMPCB using anti-PMPCB antibody (M11793). PMPCB was detected in a paraffin-embedded section of human adenocarcinoma of lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PMPCB Antibody (M11793) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11793-pmpcb-primary-antibodies-ihc-testing-3.jpgAnti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) IHC analysis of PMPCB using anti-PMPCB antibody (M11793). PMPCB was detected in a paraffin-embedded section of human ovarian carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PMPCB Antibody (M11793) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11793-pmpcb-primary-antibodies-if-testing-4.jpgAnti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) IF analysis of PMPCB using anti-PMPCB antibody (M11793). PMPCB was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-PMPCB Antibody (M11793) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11793-pmpcb-primary-antibodies-fcm-testing-5.jpgAnti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) Flow Cytometry analysis of JK cells using anti-PMPCB antibody (M11793). Overlay histogram showing JK cells stained with M11793 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PMPCB Antibody (M11793, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erlin-2-erlin2-picoband-trade-antibody-m07042-1-boster.html2026-04-06T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-1-erlin2-primary-antibodies-wb-testing-1.jpgAnti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) Western blot analysis of ERLIN2 using anti-ERLIN2 antibody (M07042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T47D whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ERLIN2 antigen affinity purified monoclonal antibody (Catalog # M07042-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ERLIN2 at approximately 43 kDa. The expected band size for ERLIN2 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-1-erlin2-primary-antibodies-ihc-testing-2.jpgAnti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) IHC analysis of ERLIN2 using anti-ERLIN2 antibody (M07042-1). ERLIN2 was detected in a paraffin-embedded section of human ovarian serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ERLIN2 Antibody (M07042-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-1-erlin2-primary-antibodies-ihc-testing-3.jpgAnti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) IHC analysis of ERLIN2 using anti-ERLIN2 antibody (M07042-1). ERLIN2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ERLIN2 Antibody (M07042-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-1-erlin2-primary-antibodies-ihc-testing-4.jpgAnti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) IHC analysis of ERLIN2 using anti-ERLIN2 antibody (M07042-1). ERLIN2 was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ERLIN2 Antibody (M07042-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-1-erlin2-primary-antibodies-ihc-testing-5.jpgAnti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) IHC analysis of ERLIN2 using anti-ERLIN2 antibody (M07042-1). ERLIN2 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ERLIN2 Antibody (M07042-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-1-erlin2-primary-antibodies-if-testing-6.jpgAnti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) IF analysis of ERLIN2 using anti-ERLIN2 antibody (M07042-1). ERLIN2 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-ERLIN2 Antibody (M07042-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07042-1-erlin2-primary-antibodies-fcm-testing-7.jpgAnti-Erlin-2/ERLIN2 Antibody Picoband® (monoclonal, 3H9A2) Flow Cytometry analysis of JK cells using anti-ERLIN2 antibody (M07042-1). Overlay histogram showing JK cells stained with M07042-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ERLIN2 Antibody (M07042-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-integrin-beta-4-itgb4-picoband-trade-antibody-m01015-2-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01015-2-itgb4-primary-antibodies-wb-testing-1.jpgAnti-Integrin beta 4/ITGB4 Antibody Picoband® (monoclonal, 7G10D2) Western blot analysis of ITGB4 using anti-ITGB4 antibody (M01015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ITGB4 antigen affinity purified monoclonal antibody (Catalog # M01015-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ITGB4 at approximately 210 kDa. The expected band size for ITGB4 is at 202 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01015-2-itgb4-primary-antibodies-ihc-testing-2.jpgAnti-Integrin beta 4/ITGB4 Antibody Picoband® (monoclonal, 7G10D2) IF analysis of ITGB4 using anti-ITGB4 antibody (M01015-2). ITGB4 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-ITGB4 Antibody (M01015-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01015-2-itgb4-primary-antibodies-fcm-testing-3.jpgAnti-Integrin beta 4/ITGB4 Antibody Picoband® (monoclonal, 7G10D2) Flow Cytometry analysis of MCF-7 cells using anti-ITGB4 antibody (M01015-2). Overlay histogram showing MCF-7 cells stained with M01015-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ITGB4 Antibody (M01015-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm7-picoband-trade-antibody-m01649-3-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-3-mcm7-primary-antibodies-wb-testing-1.jpgAnti-MCM7 Antibody Picoband® (monoclonal, 3H11) Western blot analysis of MCM7 using anti-MCM7 antibody (M01649-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MDA-MB-231 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MCM7 antigen affinity purified monoclonal antibody (Catalog # M01649-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-3-mcm7-primary-antibodies-wb-testing-2.jpgAnti-MCM7 Antibody Picoband® (monoclonal, 3H11) Western blot analysis of MCM7 using anti-MCM7 antibody (M01649-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MCM7 antigen affinity purified monoclonal antibody (Catalog # M01649-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-DyLight 647 secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-3-mcm7-primary-antibodies-ihc-testing-3.jpgAnti-MCM7 Antibody Picoband® (monoclonal, 3H11) IHC analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MCM7 Antibody (M01649-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-3-mcm7-primary-antibodies-ihc-testing-4.jpgAnti-MCM7 Antibody Picoband® (monoclonal, 3H11) IHC analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in a paraffin-embedded section of human adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MCM7 Antibody (M01649-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-3-mcm7-primary-antibodies-ihc-testing-5.jpgAnti-MCM7 Antibody Picoband® (monoclonal, 3H11) IHC analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MCM7 Antibody (M01649-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-3-mcm7-primary-antibodies-fcm-testing-7.jpgAnti-MCM7 Antibody Picoband® (monoclonal, 3H11) Flow Cytometry analysis of MCF-7 cells using anti-MCM7 antibody (M01649-3). Overlay histogram showing MCF-7 cells stained with M01649-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MCM7 Antibody (M01649-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01649-3-mcm7-primary-antibodies-if-testing-6.jpgAnti-MCM7 Antibody Picoband® (monoclonal, 3H11) IF analysis of MCM7 using anti-MCM7 antibody (M01649-3). MCM7 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-MCM7 Antibody (M01649-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cyclin-e1-ccne1-picoband-trade-antibody-m00543-3-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00543-3-ccne1-primary-antibodies-wb-testing-1.jpgAnti-Cyclin E1/CCNE1 Antibody Picoband® (monoclonal, 8B9C3) Western blot analysis of CCNE1 using anti-CCNE1 antibody (M00543-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CCNE1 antigen affinity purified monoclonal antibody (Catalog # M00543-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CCNE1 at approximately 47 kDa. The expected band size for CCNE1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-talin-1-tln1-picoband-trade-antibody-m02859-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02859-tln1-primary-antibodies-wb-testing-1.jpgAnti-Talin 1/TLN1 Antibody Picoband® (monoclonal, 2G10D2) Western blot analysis of TLN1 using anti-TLN1 antibody (M02859). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TLN1 antigen affinity purified monoclonal antibody (Catalog # M02859) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TLN1 at approximately 250-270 kDa. The expected band size for TLN1 is at 270 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02859-tln1-primary-antibodies-fcm-testing-2.jpgAnti-Talin 1/TLN1 Antibody Picoband® (monoclonal, 2G10D2) Flow Cytometry analysis of JK cells using anti-TLN1 antibody (M02859). Overlay histogram showing JK cells stained with M02859 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TLN1 Antibody (M02859, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rcc1-picoband-trade-antibody-m02719-1-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-1-rcc1-primary-antibodies-wb-testing-1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 7B5D2) Western blot analysis of RCC1 using anti-RCC1 antibody (M02719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RCC1 antigen affinity purified monoclonal antibody (Catalog # M02719-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RCC1 at approximately 47 kDa. The expected band size for RCC1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rcc1-picoband-trade-antibody-m02719-2-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-2-rcc1-primary-antibodies-wb-testing-1_1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 6B11E7) Western blot analysis of RCC1 using anti-RCC1 antibody (M02719-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RCC1 antigen affinity purified monoclonal antibody (Catalog # M02719-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RCC1 at approximately 47 kDa. The expected band size for RCC1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-2-rcc1-primary-antibodies-ihc-testing-2_1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 6B11E7) IHC analysis of RCC1 using anti-RCC1 antibody (M02719-2). RCC1 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RCC1 Antibody (M02719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-2-rcc1-primary-antibodies-ihc-testing-3_1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 6B11E7) IHC analysis of RCC1 using anti-RCC1 antibody (M02719-2). RCC1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RCC1 Antibody (M02719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-2-rcc1-primary-antibodies-ihc-testing-4_1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 6B11E7) IHC analysis of RCC1 using anti-RCC1 antibody (M02719-2). RCC1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RCC1 Antibody (M02719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-2-rcc1-primary-antibodies-ihc-testing-5_1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 6B11E7) IHC analysis of RCC1 using anti-RCC1 antibody (M02719-2). RCC1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RCC1 Antibody (M02719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-2-rcc1-primary-antibodies-ihc-testing-6_1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 6B11E7) IHC analysis of RCC1 using anti-RCC1 antibody (M02719-2). RCC1 was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RCC1 Antibody (M02719-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02719-2-rcc1-primary-antibodies-if-testing-7_1.jpgAnti-RCC1 Antibody Picoband® (monoclonal, 6B11E7) IF analysis of RCC1 using anti-RCC1 antibody (M02719-2). RCC1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-RCC1 Antibody (M02719-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-khsrp-picoband-trade-antibody-m02770-3-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02770-3-khsrp-primary-antibodies-wb-testing-1.jpgAnti-KHSRP Antibody Picoband® (monoclonal, 4F10D2) Western blot analysis of KHSRP using anti-KHSRP antibody (M02770-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KHSRP antigen affinity purified monoclonal antibody (Catalog # M02770-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KHSRP at approximately 82 kDa. The expected band size for KHSRP is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02770-3-khsrp-primary-antibodies-if-testing-2.jpgAnti-KHSRP Antibody Picoband® (monoclonal, 4F10D2) IF analysis of KHSRP using anti-KHSRP antibody (M02770-3). KHSRP was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-KHSRP Antibody (M02770-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02770-3-khsrp-primary-antibodies-fcm-testing-3.jpgAnti-KHSRP Antibody Picoband® (monoclonal, 4F10D2) Flow Cytometry analysis of U251 cells using anti-KHSRP antibody (M02770-3). Overlay histogram showing U251 cells stained with M02770-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-KHSRP Antibody (M02770-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kpnb1-picoband-trade-antibody-m01851-2-boster.html2026-03-17T05:13:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-wb-testing-1_1.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) Western blot analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KPNB1 antigen affinity purified monoclonal antibody (M01851-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-DyLight 647 secondary antibody (Catalog # BA1151) at a dilution of 1:2000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KPNB1 at approximately 97 kDa. The expected band size for KPNB1 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-2.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-3.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-4.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-5.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-6.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-7.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-8.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-9.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-10.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-11.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-ihc-testing-12.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IHC analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-if-testing-13.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) IF analysis of KPNB1 using anti-KPNB1 antibody (M01851-2). KPNB1 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-KPNB1 Antibody (M01851-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01851-2-kpnb1-primary-antibodies-fcm-testing-14.jpgAnti-KPNB1 Antibody Picoband® (monoclonal, 3I11F2) Flow Cytometry analysis of RT4 cells using anti-KPNB1 antibody (M01851-2). Overlay histogram showing RT4 cells stained with M01851-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-KPNB1 Antibody (M01851-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-flotillin-2-picoband-trade-antibody-m06107-2-boster.html2026-03-25T05:24:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06107-2-flot2-primary-antibodies-wb-testing-1.jpgAnti-Flotillin 2 Antibody Picoband® (monoclonal, 4D8A3) Western blot analysis of Flotillin 2 using anti-Flotillin 2 antibody (M06107-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Flotillin 2 antigen affinity purified monoclonal antibody (Catalog # M06107-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Flotillin 2 at approximately 49 kDa. The expected band size for Flotillin 2 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06107-2-flot2-primary-antibodies-ihc-testing-2.jpgAnti-Flotillin 2 Antibody Picoband® (monoclonal, 4D8A3) IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (M06107-2). Flotillin 2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Flotillin 2 Antibody (M06107-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06107-2-flot2-primary-antibodies-if-testing-3.jpgAnti-Flotillin 2 Antibody Picoband® (monoclonal, 4D8A3) IF analysis of FLOT2 using anti-FLOT2 antibody (M06107-2). FLOT2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-FLOT2 Antibody (M06107-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-delta-1-catenin-cas-ctnnd1-picoband-trade-antibody-m02333-2-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-2-ctnnd1-primary-antibodies-wb-testing-1_1.jpgAnti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband® (monoclonal, 8G7E4) Western blot analysis of CTNND1 using anti-CTNND1 antibody (M02333-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CTNND1 antigen affinity purified monoclonal antibody (Catalog # M02333-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CTNND1 at approximately 100 kDa. The expected band size for CTNND1 is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-2-ctnnd1-primary-antibodies-ihc-testing-2.jpgAnti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband® (monoclonal, 8G7E4) IHC analysis of CTNND1 using anti-CTNND1 antibody (M02333-2). CTNND1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTNND1 Antibody (M02333-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-2-ctnnd1-primary-antibodies-ihc-testing-3.jpgAnti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband® (monoclonal, 8G7E4) IHC analysis of CTNND1 using anti-CTNND1 antibody (M02333-2). CTNND1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTNND1 Antibody (M02333-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-2-ctnnd1-primary-antibodies-ihc-testing-4.jpgAnti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband® (monoclonal, 8G7E4) IHC analysis of CTNND1 using anti-CTNND1 antibody (M02333-2). CTNND1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTNND1 Antibody (M02333-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-2-ctnnd1-primary-antibodies-if-testing-5.jpgAnti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband® (monoclonal, 8G7E4) IF analysis of CTNND1 using anti-CTNND1 antibody (M02333-2). CTNND1 was detected in an immunocytochemical section of RT4 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-CTNND1 Antibody (M02333-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trim24-picoband-trade-antibody-m03258-2-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03258-2-trim24-primary-antibodies-wb-testing-1.jpgAnti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) Western blot analysis of TRIM24 using anti-TRIM24 antibody (M03258-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TRIM24 antigen affinity purified monoclonal antibody (Catalog # M03258-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TRIM24 at approximately 130 kDa. The expected band size for TRIM24 is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03258-2-trim24-primary-antibodies-ihc-testing-2.jpgAnti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2). TRIM24 was detected in a paraffin-embedded section of human adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03258-2-trim24-primary-antibodies-ihc-testing-3.jpgAnti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2). TRIM24 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03258-2-trim24-primary-antibodies-ihc-testing-4.jpgAnti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2). TRIM24 was detected in a paraffin-embedded section of human ovarian serous tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03258-2-trim24-primary-antibodies-ihc-testing-5.jpgAnti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2). TRIM24 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03258-2-trim24-primary-antibodies-ihc-testing-6.jpgAnti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) IF analysis of TRIM24 using anti-TRIM24 antibody (M03258-2). TRIM24 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a3-picoband-trade-antibody-m04796-2-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04796-2-anxa3-primary-antibodies-wb-testing-1.jpgAnti-Annexin A3 Antibody Picoband® (monoclonal, 2H3H8) Western blot analysis of Annexin A3 using anti-Annexin A3 antibody (M04796-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Annexin A3 antigen affinity purified monoclonal antibody (Catalog # M04796-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Annexin A3 at approximately 36 kDa. The expected band size for Annexin A3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04796-2-anxa3-primary-antibodies-ihc-testing-2.jpgAnti-Annexin A3 Antibody Picoband® (monoclonal, 2H3H8) IHC analysis of Annexin A3 using anti-Annexin A3 antibody (M04796-2). Annexin A3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A3 Antibody (M04796-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04796-2-anxa3-primary-antibodies-ihc-testing-3.jpgAnti-Annexin A3 Antibody Picoband® (monoclonal, 2H3H8) IHC analysis of Annexin A3 using anti-Annexin A3 antibody (M04796-2). Annexin A3 was detected in a paraffin-embedded section of human adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A3 Antibody (M04796-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04796-2-anxa3-primary-antibodies-ihc-testing-4.jpgAnti-Annexin A3 Antibody Picoband® (monoclonal, 2H3H8) IHC analysis of Annexin A3 using anti-Annexin A3 antibody (M04796-2). Annexin A3 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A3 Antibody (M04796-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04796-2-anxa3-primary-antibodies-fcm-testing-5.jpgAnti-Annexin A3 Antibody Picoband® (monoclonal, 2H3H8) Flow Cytometry analysis of HepG2 cells using anti-Annexin A3 antibody (M04796-2). Overlay histogram showing HepG2 cells stained with M04796-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-Annexin A3 Antibody (M04796-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hla-dr-hla-dra-picoband-trade-antibody-m01195-3-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-3-hla-dr-primary-antibodies-wb-testing-1.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1) Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HLA-DRA antigen affinity purified monoclonal antibody (Catalog # M01195-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HLA-DRA at approximately 35-37 kDa. The expected band size for HLA-DRA is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-3-hla-dr-primary-antibodies-ihc-testing-2.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-3). HLA-DRA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-3-hla-dr-primary-antibodies-ihc-testing-3.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-3). HLA-DRA was detected in a paraffin-embedded section of human mammary infiltrate tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-3-hla-dr-primary-antibodies-ihc-testing-4.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-3). HLA-DRA was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-3-hla-dr-primary-antibodies-ihc-testing-5.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-3). HLA-DRA was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-3-hla-dr-primary-antibodies-fcm-testing-6.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 8I10H1) Flow Cytometry analysis of Daudi cells using anti-HLA-DRA antibody (M01195-3). Overlay histogram showing Daudi cells stained with M01195-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-HLA-DRA Antibody (M01195-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hla-dr-hla-dra-picoband-trade-antibody-m01195-4-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-4-hla-dra-primary-antibodies-wb-testing-1.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 5B13F7) Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HLA-DRA antigen affinity purified monoclonal antibody (Catalog # M01195-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HLA-DRA at approximately 35-37 kDa. The expected band size for HLA-DRA is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-4-hla-dra-primary-antibodies-ihc-testing-2.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 5B13F7) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-4). HLA-DRA was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-4-hla-dra-primary-antibodies-ihc-testing-3.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 5B13F7) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-4). HLA-DRA was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-4-hla-dra-primary-antibodies-ihc-testing-4.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 5B13F7) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-4). HLA-DRA was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-4-hla-dra-primary-antibodies-ihc-testing-5.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 5B13F7) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-4). HLA-DRA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-4-hla-dra-primary-antibodies-ihc-testing-6.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 5B13F7) IHC analysis of HLA-DRA using anti-HLA-DRA antibody (M01195-4). HLA-DRA was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-HLA-DRA Antibody (M01195-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-4-hla-dra-primary-antibodies-fcm-testing-7.jpgAnti-HLA-DR/HLA-DRA Antibody Picoband® (monoclonal, 5B13F7) Flow Cytometry analysis of Daudi cells using anti-HLA-DRA antibody (M01195-4). Overlay histogram showing Daudi cells stained with M01195-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-HLA-DRA Antibody (M01195-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ataxin-1-picoband-trade-antibody-m01786-1-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-wb-testing-1.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) Western blot analysis of Ataxin 1 using anti-Ataxin 1 antibody (M01786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ataxin 1 antigen affinity purified monoclonal antibody (Catalog # M01786-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ataxin 1 at approximately 105 kDa. The expected band size for Ataxin 1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-ihc-testing-2.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (M01786-1). Ataxin 1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Ataxin 1 Antibody (M01786-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-ihc-testing-3.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (M01786-1). Ataxin 1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Ataxin 1 Antibody (M01786-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-ihc-testing-4.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (M01786-1). Ataxin 1 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Ataxin 1 Antibody (M01786-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-ihc-testing-5.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (M01786-1). Ataxin 1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Ataxin 1 Antibody (M01786-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-ihc-testing-6.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (M01786-1). Ataxin 1 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Ataxin 1 Antibody (M01786-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-fcm-testing-7.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) Flow Cytometry analysis of PC-3 cells using anti-Ataxin 1 antibody (M01786-1). Overlay histogram showing PC-3 cells stained with M01786-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ataxin 1 Antibody (M01786-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-fcm-testing-8.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) Flow Cytometry analysis of ANA-1 cells using anti-Ataxin 1 antibody (M01786-1). Overlay histogram showing ANA-1 cells stained with M01786-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ataxin 1 Antibody (M01786-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01786-1-atxn1-primary-antibodies-fcm-testing-9.jpgAnti-Ataxin 1 Antibody Picoband® (monoclonal, 2B13G8) Flow Cytometry analysis of NRK cells using anti-Ataxin 1 antibody (M01786-1). Overlay histogram showing NRK cells stained with M01786-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ataxin 1 Antibody (M01786-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wdr1-picoband-trade-antibody-m04814-1-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04814-1-wdr1-primary-antibodies-wb-testing-1_1.jpgAnti-WDR1 Antibody Picoband® (monoclonal, 5C11C8)Western blot analysis of WDR1 using anti-WDR1 antibody (M04814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-WDR1 antigen affinity purified monoclonal antibody (Catalog # M04814-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WDR1 at approximately 66 kDa. The expected band size for WDR1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04814-1-wdr1-primary-antibodies-ihc-testing-2.jpgAnti-WDR1 Antibody Picoband® (monoclonal, 5C11C8) IHC analysis of WDR1 using anti-WDR1 antibody (M04814-1). WDR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-WDR1 Antibody (M04814-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04814-1-wdr1-primary-antibodies-ihc-testing-3.jpgAnti-WDR1 Antibody Picoband® (monoclonal, 5C11C8) IHC analysis of WDR1 using anti-WDR1 antibody (M04814-1). WDR1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-WDR1 Antibody (M04814-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glycine-decarboxylase-gldc-picoband-trade-antibody-m04777-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04777-gldc-primary-antibodies-wb-testing-1.jpgAnti-Glycine decarboxylase/GLDC Antibody Picoband® (monoclonal, 3D3D3) Western blot analysis of GLDC using anti-GLDC antibody (M04777). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GLDC antigen affinity purified monoclonal antibody (Catalog # M04777) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GLDC at approximately 113 kDa. The expected band size for GLDC is at 113 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04777-gldc-primary-antibodies-ihc-testing-2.jpgAnti-Glycine decarboxylase/GLDC Antibody Picoband® (monoclonal, 3D3D3) IHC analysis of GLDC using anti-GLDC antibody (M04777). GLDC was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-GLDC Antibody (M04777) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04777-gldc-primary-antibodies-ihc-testing-3.jpgAnti-Glycine decarboxylase/GLDC Antibody Picoband® (monoclonal, 3D3D3) IHC analysis of GLDC using anti-GLDC antibody (M04777). GLDC was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-GLDC Antibody (M04777) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sh3gl2-picoband-trade-antibody-m05430-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05430-sh3gl2-primary-antibodies-wb-testing-1.jpgAnti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1) Western blot analysis of SH3GL2 using anti-SH3GL2 antibody (M05430). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SH3GL2 antigen affinity purified monoclonal antibody (Catalog # M05430) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SH3GL2 at approximately 40 kDa. The expected band size for SH3GL2 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hsd17b14-antibody-dz41147-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41147-hsd17b14-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish hsd17b14 Antibody IHC analysis of Hsd17b14 using anti-Hsd17b14 antibody (DZ41147). Hsd17b14 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsd17b14 Antibody (DZ41147 ) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41147-hsd17b14-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish hsd17b14 Antibody IHC analysis of Hsd17b14 using anti-Hsd17b14 antibody (DZ41147). Hsd17b14 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsd17b14 Antibody (DZ41147) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cemip-antibody-dz41148-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41148-cemip-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish cemip Antibody IHC analysis of Cemip using anti-Cemip antibody (DZ41148). Cemip was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Cemip Antibody (DZ41148) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41148-cemip-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish cemip Antibody IHC analysis of Cemip using anti-Cemip antibody (DZ41148). Cemip was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Cemip Antibody (DZ41148) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-stau-antibody-dz41173-boster.html2026-03-10T04:34:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-kmo-antibody-dz41201-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41201-kmo-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish kmo Antibody Western blot analysis of Kmo using anti-Kmo antibody (DZ41201). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Marker, Lane 2: Zebrafish tissue lysates, Lane 3: Zebrafish head tissue lysates, Lane 4: Zebrafish body tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kmo antigen affinity purified polyclonal antibody (DZ41201) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kmo at approximately 54 kDa. The expected band size for Kmo is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-dc19orf12-antibody-dz41204-boster.html2026-03-10T04:34:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-vgt1-antibody-dz41206-boster.html2026-03-10T04:34:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-c-fos-antibody-dz41207-boster.html2026-03-17T05:13:26+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fission-yeast-brl1-antibody-dz41208-boster.html2026-03-10T04:34:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fission-yeast-brl2-antibody-dz41209-boster.html2026-03-10T04:34:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-baker-s-yeast-ubp8-antibody-dz41210-boster.html2026-03-10T04:34:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-clipb9-antibody-dz41213-boster.html2026-03-10T04:34:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-triatomid-bug-vtg-1-antibody-dz41215-boster.html2026-03-10T04:34:32+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-human-rhno1-antibody-dz41216-boster.html2026-03-10T04:34:32+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-japanese-scallop-kp79-pyt13592-antibody-dz41221-boster.html2026-03-10T04:34:32+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-ldh-antibody-dz41222-boster.html2026-03-10T04:34:32+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-gpdh1-antibody-dz41223-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41223-jason-tennessen.pngAnti-Fruit fly Gpdh1 Antibody IF analysis of Gpdh1 using anti-Gpdh1 antibody (DZ41223). Gpdh1 was detected in a paraffin-embedded section of Drosophila larval fat body tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-Gpdh1 Antibody (DZ41223) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-l2hgdh-antibody-dz41224-boster.html2026-03-10T04:34:32+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pcdh15b-antibody-dz41226-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41226-pcdh15b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Pcdh15b Antibody IHC analysis of Pcdh15b using anti-Pcdh15b antibody (DZ41226). Pcdh15b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Pcdh15b Antibody (DZ41226) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41226-pcdh15b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Pcdh15b Antibody IHC analysis of Pcdh15b using anti-Pcdh15b antibody (DZ41226). Pcdh15b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Pcdh15b Antibody (DZ41226) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-snai1b-antibody-dz41229-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41229-snai1b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish snai1b Antibody IHC analysis of Snai1b using anti-Snai1b antibody (DZ41229). Snai1b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Snai1b Antibody (DZ41229) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-timp2a-antibody-dz41230-boster.html2026-03-17T05:13:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41230-timp2a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish timp2a Antibody IHC analysis of Timp2a using anti-Timp2a antibody (DZ41230). Timp2a was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Timp2a Antibody (DZ41230) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41230-timp2a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish timp2a Antibody IHC analysis of Timp2a using anti-Timp2a antibody (DZ41230). Timp2a was detected in a paraffin-embedded section of zebrafish intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Timp2a Antibody (DZ41230) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41230-timp2a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish timp2a Antibody IHC analysis of Timp2a using anti-Timp2a antibody (DZ41230). Timp2a was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Timp2a Antibody (DZ41230) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41230-timp2a-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish timp2a Antibody IHC analysis of Timp2a using anti-Timp2a antibody (DZ41230). Timp2a was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Timp2a Antibody (DZ41230) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41230-timp2a-primary-antibodies-ihc-testing-5_1.jpgAnti-Zebrafish timp2a Antibody IHC analysis of Timp2a using anti-Timp2a antibody (DZ41230). Timp2a was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Timp2a Antibody (DZ41230) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-lytechinus-variegatus-dishevelled-antibody-dz41232-boster.html2026-03-10T04:34:33+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myomaker-antibody-dz41236-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41236-myomaker-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Myomaker Antibody IHC analysis of Myomixer using anti-Myomixerer antibody (DZ41237). Myomixer was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Myomixer Antibody (DZ41237) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myomixer-antibody-dz41237-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41237-myomixer-primary-antibodies-ihc-testing-1_1.jpgAnti-Zebrafish Myomixer Antibody IHC analysis of Myomixer using anti-Myomixerer antibody (DZ41237). Myomixer was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Myomixer Antibody (DZ41237) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-bacteriophage-t4-gp23-antibody-dz41238-boster.html2026-03-10T04:34:33+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-egfp-antibody-dz41239-boster.html2026-03-10T04:34:34+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-rim-antibody-dz41240-boster.html2026-03-10T04:34:34+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-unc13b-antibody-dz41241-boster.html2026-03-10T04:34:34+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-endophilin-a-antibody-dz41242-boster.html2026-03-10T04:34:34+00:00daily0.9 https://www.bosterbio.com/picokine-elisa-kits/human-dscaml1-picokine-elisa-kit-ek2188-boster.html2026-03-10T04:34:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2188.pngHuman DSCAML1 ELISA Kit PicoKine®Human DSCAML1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dsg2-picokine-elisa-kit-ek2189-boster.html2026-03-10T04:34:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2189.jpgHuman DSG2 ELISA Kit PicoKine®Human DSG2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dsg3-picokine-elisa-kit-ek2190-boster.html2026-03-10T04:34:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2190.jpgHuman DSG3 ELISA Kit PicoKine®Human DSG3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-neto-alpha-antibody-dz41243-boster.html2026-03-10T04:34:35+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-hydractinia-symbiolongicarpus-piwi1-antibody-dz41247-boster.html2026-03-10T04:34:35+00:00daily0.9 https://www.bosterbio.com/picokine-elisa-kits/human-enpp7-picokine-elisa-kit-ek2191-boster.html2026-03-10T04:34:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2191.jpgHuman ENPP7 ELISA Kit PicoKine®Human ENPP7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nemp1-antibody-dz41248-boster.html2026-03-17T05:13:27+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nemp2-antibody-dz41249-boster.html2026-03-17T05:13:27+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-nemp-antibody-dz41250-boster.html2026-03-10T04:34:35+00:00daily0.9 https://www.bosterbio.com/picokine-elisa-kits/human-calsyntenin-2-picokine-elisa-kit-ek2193-boster.html2026-03-10T04:34:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2193.pngHuman Calsyntenin 2 ELISA Kit PicoKine®Human Calsyntenin 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-mal-b1-antibody-dz41251-boster.html2026-03-10T04:34:35+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-npc2c-antibody-dz41252-boster.html2026-03-10T04:34:36+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-spn-a-antibody-dz41253-boster.html2026-03-10T04:34:36+00:00daily0.9 https://www.bosterbio.com/picokine-elisa-kits/human-cd46-picokine-elisa-kit-ek2194-boster.html2026-03-10T04:34:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2194.jpgHuman CD46 ELISA Kit PicoKine®Human CD46 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-mei-217-antibody-dz41254-boster.html2026-03-10T04:34:36+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-mei-218-antibody-dz41255-boster.html2026-03-10T04:34:36+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-pabp-antibody-dz41256-boster.html2026-03-25T05:24:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-tral-antibody-dz41257-boster.html2026-03-25T05:24:32+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-bicc-antibody-dz41258-boster.html2026-03-10T04:34:36+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-col12a1a-antibody-dz41260-boster.html2026-04-01T05:01:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41260-col12a1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish col12a1a Antibody IHC analysis of Col12a1a using anti-Col12a1a antibody (DZ41260). Col12a1a was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Col12a1a Antibody (DZ41260) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41260-41467_2025_59169_fig7_html.pngAnti-Zebrafish col12a1a AntibodyMmp14b is a regulator of CM protrusion. A Collagen hybridizing peptide (CHP) and phalloidin staining of a wild-type ventricle at 10 dpci. Yellow box denotes the area in the zoomed image. B In situ hybridization of mmp14b expression in a wild-type ventricle at 7 dpci. Black dashed line denotes the approximate injury border. C Colocalization of mmp14b (HCR, magenta) with CMs (MHC immunostaining), endocardial cells (vwf HCR), macrophages (GFP immunostaining in Tg(mpeg1:EGFP) ventricles, and fibroblasts ( col12a1a (HCR, green) and postnb (HCR, cyan) in the cortical BZ region at 10 dpci. Yellow box in the schematic of the heart marks the cortical CM region depicted in the zoomed images. Created in BioRender. Beisaw, A. (2025) . D Schematic depicting the exon structure of the mmp14b locus and CRISPR/Cas9-induced full-length deletion between exons 2 and 9 of mmp14b . Created in BioRender. Beisaw, A. (2025) . E Mmp14b wild-type and putative mutant protein domain structure (left). RT-PCR of the mmp14b open reading frame from wild-type and mmp14b Δ/Δ mutant embryos (right). SP signal peptide, Pro propeptide, Cat catalytic domain, H hinge region, TM transmembrane domain, C C-terminal tail. F RT-qPCR of mmp14b and mmp14a expression in single ventricles from mmp14b Δ/Δ ( n = 5 ventricles) and wild-type siblings ( n = 5 ventricles) at 10 dpci. Data are presented as mean ± SD. P -values were calculated using an unpaired two-sided t -test. Source data are presented in the Source Data file. G Phalloidin staining of thick cryosections from mmp14b Δ/Δ and wild-type sibling ventricles at 10 dpci (left). Quantification of CM protrusion length (right, mmp14b Δ/Δ n = 1124 CM protrusions from 8 ventricles, wild-type sibling n = 1480 CM protrusions from 9 ventricles). Data are presented as violin plots of all points with solid gray lines indicating the median and dotted gray lines indicating 25th and 75th percentile. P -values were calculated using a two-sided Mann–Whitney test. Source data are presented in the Source Data file. H Picrosirius red staining of collagen in mmp14b Δ/Δ ( n = 8 ventricles) and wild-type sibling ( n = 10 ventricles) at 60 dpci (left). Quantification of scar area (% of ventricle area) on the right. Data are presented as mean ± SD. P -value was calculated using an unpaired two-sided t -test. Source data are presented in the Source Data file. I Quantification of CM proliferation within 100 μm of the wound border from PCNA/Mef2 immunostaining in mmp14b Δ/Δ ( n = 3 ventricles) and wild-type sibling ( n = 4 ventricles) at 7 dpci. Data are presented as mean ± SD. P -value was calculated using an unpaired two-sided t -test. Source data are presented in the Source Data file. Scale bars: 100 μm in ( A , B , G , and H ), 20 μm in zoomed image in ( A and C ). Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59169-4'>40268967</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41260-41467_2025_59169_fig8_html.pngAnti-Zebrafish col12a1a AntibodyMmp14b is essential for macrophage presence and ECM remodeling at the border zone. A Collagen hybridizing peptide (CHP) and mScarlet immunostaining in Tg(mpeg1:EGFP); Tg(myl7:lck-mScarlet) ventricles from mmp14b Δ/Δ and wild-type siblings at 10 dpci. White dashed lines indicate the wound border and yellow boxes contain zoomed images from the wound border zone. B Quantification of CHP intensity at the border zone (arb. units, arbitrary units) in Tg(mpeg1:EGFP); Tg(myl7:lck-mScarlet) ventricles from mmp14b Δ/Δ ( n = 5 ventricles) and wild-type siblings ( n = 4 ventricles) at 10 dpci. Data are presented as mean ± SD. P -value was calculated using an unpaired two-sided t -test. Source data are presented in the Source Data file. C GFP and mScarlet immunostaining in Tg(mpeg1:EGFP); Tg(myl7:lck-mScarlet) ventricles from mmp14b Δ/Δ and wild-type siblings at 10 dpci. White dashed lines indicate the approximate wound border. D Quantification of mpeg1 :EGFP+ cells 50 μm proximal and distal to the wound border in ventricles from mmp14b Δ/Δ ( n = 6 ventricles) and wild-type siblings (7 dpci n = 7 ventricles, 10 dpci n = 6 ventricles) at 7 and 10 dpci. Data are presented as mean ± SD. P -values were calculated using unpaired two-sided t -tests and corrected for multiple comparisons using the Holm-Sidak method. Source data are presented in the Source Data file. E RT-qPCR analysis of fibroblast marker genes in mmp14b Δ/Δ mutant ( n = 5 ventricles) and wild-type sibling ( n = 5 ventricles) at 10 dpci. Data are presented as mean ± SD. P -values were calculated using an unpaired two-sided t -test or a two-sided Mann–Whitney test ( col1a1a ). Source data are presented in the Source Data file. F col12a1a in situ hybridization chain reaction (HCR) in mmp14b Δ/Δ mutant and wild-type sibling ventricles at 10 dpci. Yellow boxes denote zoomed images at the cortical BZ, blue boxes denote zoomed images at the apex of the wound. Epi, epicardium. Scale bars: 100 μm, 20 μm in the zoomed images in ( F ). Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-59169-4'>40268967</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41260-col12a1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish col12a1a Antibody IHC analysis of Col12a1a using anti-Col12a1a antibody (DZ41260). Col12a1a was detected in a paraffin-embedded section of zebrafish gill tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Col12a1a Antibody (DZ41260) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-alk-antibody-dz41261-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41261-alk-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish alk Antibody IHC analysis of Alk using anti-Alk antibody (DZ41261). Alk was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Alk Antibody (DZ41261) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-tick-borne-encephalitis-virus-genome-polyprotein-antibody-dz41264-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-cg4115-antibody-dz41265-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-cg43333-antibody-dz41266-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-yellowfever-mosquito-ctlma15-antibody-dz41274-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-s-cerevisiae-uwops05-227-2-drt2-antibody-dz41275-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-clawed-frog-cdc42ep1-antibody-dz41276-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-clawed-frog-versican-antibody-dz41277-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-clawed-frog-kdr-antibody-dz41278-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-human-ervw-1-antibody-dz41279-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-wheat-dna-ligase-iv-antibody-dz41283-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tyrosine-hydrxylase-th-antibody-dz41285-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41285-th-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish tyrosine hydrxylase /th Antibody Western blot analysis of Tyrosine Hydrxylase/Th using anti-Tyrosine Hydrxylase/Th antibody (DZ41285). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Marker, Lane 2: Zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosine Hydrxylase/Th antigen affinity purified polyclonal antibody (DZ41285) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosine Hydrxylase/Th at approximately 55 kDa. The expected band size for Tyrosine Hydrxylase/Th is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41285-th-primary-antibodies-ihc-testing-2_1.jpgAnti-Zebrafish tyrosine hydrxylase /th Antibody IHC analysis of Tyrosine Hydrxylase/Th using anti-Tyrosine Hydrxylase/Th antibody (DZ41285). Tyrosine Hydrxylase/Th was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydrxylase/Th Antibody (DZ41285) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41285-th-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish tyrosine hydrxylase /th AntibodyIHC analysis of Tyrosine Hydrxylase/Th using anti-Tyrosine Hydrxylase/Th antibody (DZ41285). Tyrosine Hydrxylase/Th was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydrxylase/Th Antibody (DZ41285) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41285-th-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish tyrosine hydrxylase /th Antibody IHC analysis of Tyrosine Hydrxylase/Th using anti-Tyrosine Hydrxylase/Th antibody (DZ41285). Tyrosine Hydrxylase/Th was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydrxylase/Th Antibody (DZ41285) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41285-th-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish tyrosine hydrxylase /th AntibodyIHC analysis of Tyrosine Hydrxylase/Th using anti-Tyrosine Hydrxylase/Th antibody (DZ41285). Tyrosine Hydrxylase/Th was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydrxylase/Th Antibody (DZ41285) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-chata-antibody-dz41286-boster.html2026-04-05T05:00:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-cg1113-antibody-dz41287-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-stra6-antibody-dz41288-boster.html2026-03-17T05:13:27+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-prox1a-antibody-dz41290-boster.html2026-03-17T05:13:27+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-blm-antibody-dz41293-boster.html2026-03-10T04:34:37+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-casc3-antibody-dz41294-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41294-casc3-primary-antibodies-wb-testing-1_1.jpgAnti-Zebrafish Casc3 Antibody Western blot analysis of Casc3 using anti-Casc3 antibody (DZ41294). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Marker, Lane 2: Zebrafish, Lane 3: Zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Casc3 antigen affinity purified polyclonal antibody (DZ41294) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Casc3 at approximately 76 kDa. The expected band size for Casc3 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41294-casc3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Casc3 Antibody IHC analysis of Casc3 using anti-Casc3 antibody (DZ41294). Casc3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Casc3 Antibody (DZ41294) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-sxc-antibody-dz41296-boster.html2026-03-10T04:34:38+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-nup62-antibody-dz41297-boster.html2026-03-10T04:34:38+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-mouse-msh4-antibody-dz41305-boster.html2026-03-10T04:34:38+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-human-ccne1-antibody-dz41310-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41310-ccne1-primary-antibodies-wb-testing-1.pngAnti-Human CCNE1 AntibodyWestern blot analysis of CCNE1 using anti-CCNE1 antibody (DZ41310). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HaCaT whole cell lysates, Lane 2: human A2780cp20 whole cell lysates, Lane 3: human HCT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 antigen affinity purified polyclonal antibody (DZ41310) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Anti-rabbit/mouse IgG horseradish peroxidase-conjugated at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Biorad Chemidoc system. A specific band was detected for CCNE1 at approximately 50 kDa. The expected band size for CCNE1 is at 47 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41310-kasturi_mitra.pngAnti-Human CCNE1 AntibodyIF analysis of CCNE1 using anti-CCNE1 antibody (DZ41310). CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41310) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-human-ccne1-antibody-dz41311-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41311-kasturi_mitra.pngAnti-Human CCNE1 AntibodyIF analysis of CCNE1 using anti-CCNE1 antibody (DZ41311). CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41311) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Confocal Microscope Zeiss LSM 900 and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-human-ccne1-antibody-dz41312-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41312-kasturi_mitra.pngAnti-Human CCNE1 AntibodyIF analysis of CCNE1 using anti-CCNE1 antibody (DZ41312). CCNE1 was detected in an immunocytochemical section of HaCaT cells. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour. And then incubated with 1:100-1:200 rabbit anti-CCNE1 Antibody (DZ41312) overnight at 4°C. Goat anti-rabbit IgG conjugated to Alexa Fluor 488 was used as secondary antibody at 1:1000 dilution and incubated for 1-2 hours at RT. The section was counterstained with DAPI. Visualize using Confocal Microscope Zeiss LSM 900 and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-sod2-antibody-dz41313-boster.html2026-03-10T04:34:38+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-drp1-antibody-dz41314-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41314-kasturi_mitra-icc.pngAnti-Fruit fly Drp1 AntibodyIF analysis of Drp1 using anti-Drp1 antibody (DZ41314). Drp1 was detected in a paraffin-embedded section of Drosophila Fat body tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:100 rabbit anti-Drp1 Antibody (DZ41314) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41314-kasturi_mitra-wb.pngAnti-Fruit fly Drp1 AntibodyThe figure shows a Western blot of Drosophila ovaries stained for Drp1 in a Gal4 control line and a Drp1 RNAi. A reduction in Drp1 levels is observed at ~85 kDa in the RNAi sample, as indicated by the molecular weight ladder. β-Actin was used as a loading control. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-marf-antibody-dz41315-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41315-kasturi_mitra-if-2.pngAnti-Fruit fly Marf AntibodyIF analysis of Marf using anti-Marf antibody (DZ41315). Marf was detected in a paraffin-embedded section of Drosophila Fat body tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:200 rabbit anti-Marf Antibody (DZ41315) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41315-kasturi_mitra-if.pngAnti-Fruit fly Marf AntibodyThe image shows immunohistochemistry of the Drosophila germarium stained for ATPβ (a mitochondrial marker), Marf, and nuclei (Hoechst). The similarity between Marf and ATPβ suggests that Marf is mitochondrially localised. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-opa1-antibody-dz41316-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41316-if.pngAnti-Fruit fly Opa1 AntibodyIF analysis of Opa1 using anti-Opa1 antibody (DZ41316). Opa1 was detected in a paraffin-embedded section of Drosophila ovary tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:100 rabbit anti-Opa1 Antibody (DZ41316) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-phb2-antibody-dz41317-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41317-kasturi_mitra-if.pngAnti-Fruit fly Phb2 AntibodyIF analysis of Phb2 using anti-Phb2 antibody (DZ41317). Phb2 was detected in a paraffin-embedded section of Drosophila fat body tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:100 rabbit anti-Phb2 Antibody (DZ41317) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41317-kasturi_mitra-if-2.pngAnti-Fruit fly Phb2 AntibodyIF analysis of Phb2 using anti-Phb2 antibody (DZ41317). Phb2 was detected in a paraffin-embedded section of Drosophila ovary tissue. The tissues were fixed with 4% PFA and permeabilized in PBS-TX. And then incubated with 1:100 rabbit anti-Phb2 Antibody (DZ41317) in 2% BSA and 0.1% PBS-TX overnight at 4°C. Anti-Rabbit/Mouse Alexa Fluor 488/Cy3/Cy5 was used as secondary antibody at 1:1000 dilution and incubated for 1 hours at RT. Visualize using Zeiss LSM 900 Confocal Microscope with Airyscan 2 and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41317-kasturi_mitra-wb.pngAnti-Fruit fly Phb2 AntibodyThe figure shows a Western blot of Phb2 in Drosophila ovaries from 2 fly strains. Phb2 protein bands are observed at 30 kDa, as expected. β-Actin was used as a loading control. https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/human-tff2-elisa-kit-ez-set-trade-diy-antibody-pairs-ez1233-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez1233.pngHuman TFF2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human TFF2 EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-1-beta-il1b-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0394-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0394.pngMouse IL-1 Beta/IL-1F2/IL1B ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-1 Beta EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-ctla4-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0717-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0717.pngMouse CTLA4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse CTLA4 EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-cd69-elisa-kit-ez-set-trade-diy-antibody-pairs-ez2053-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez2053.pngMouse CD69 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse CD69 EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-1-alpha-il-1f1-il1a-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0391-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0391.pngMouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-1 Alpha EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-2-interleukin-2-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0398-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0398.pngMouse IL-2/Interleukin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-2 EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-3-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0403-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0403.pngMouse IL-3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-3 EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-12-p70-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0422-boster.html2026-03-25T05:24:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0422.pngMouse IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-12 (p70) EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-17f-interleukin-17f-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0796-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0796.jpgMouse IL-17F/Interleukin-17F ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-17F EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-linked-ilk-picoband-trade-antibody-m02932-2-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02932-2-ilk-primary-antibodies-wb-testing-1.jpgAnti-Integrin linked ILK Antibody Picoband® (monoclonal, 3C7E1) Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (M02932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat heart tissue lysates. Lane 5: rat kidney tissue lysates. Lane 6: mouse heart tissue lysates. Lane 7: mosue kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Integrin linked ILK antigen affinity purified monoclonal antibody (Catalog # M02932-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 51 kDa. The expected band size for Integrin linked ILK is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02932-2-ilk-primary-antibodies-ihc-testing-2.jpgAnti-Integrin linked ILK Antibody Picoband® (monoclonal, 3C7E1) IHC analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (M02932-2). Integrin linked ILK was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Integrin linked ILK Antibody (M02932-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02932-2-ilk-primary-antibodies-ihc-testing-3.jpgAnti-Integrin linked ILK Antibody Picoband® (monoclonal, 3C7E1) IHC analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (M02932-2). Integrin linked ILK was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Integrin linked ILK Antibody (M02932-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02932-2-ilk-primary-antibodies-ihc-testing-4.jpgAnti-Integrin linked ILK Antibody Picoband® (monoclonal, 3C7E1) IHC analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (M02932-2). Integrin linked ILK was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Integrin linked ILK Antibody (M02932-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-il-27-p28-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0800-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0800.pngMouse IL-27 p28 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse IL-27 p28 EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/ez-set-elisa-kits-antibody-pairs/mouse-tnf-alpha-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0527-boster.html2026-03-10T04:34:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0527.pngMouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs)Mouse TNF EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-fis1-antibody-dz41318-boster.html2026-03-10T04:34:39+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sall1a-antibody-dz41319-boster.html2026-03-17T05:13:27+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-crithidia-fasciculata-gp63-adherent-antibody-dz41320-boster.html2026-03-10T04:34:39+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-crithidia-fasciculata-gp63-swimmer-antibody-dz41321-boster.html2026-03-10T04:34:39+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-yellowfever-mosquito-transferrin-antibody-dz41323-boster.html2026-03-10T04:34:39+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-isg15-antibody-dz41332-boster.html2026-03-17T05:13:27+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-tep3-antibody-dz41333-boster.html2026-03-10T04:34:39+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-tep15-antibody-dz41334-boster.html2026-03-10T04:34:39+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-abhd4-picoband-trade-antibody-a14435-1-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14435-1-abhd4-primary-antibodies-wb-testing-1.jpgAnti-ABHD4 Antibody Picoband® Western blot analysis of ABHD4 using anti-ABHD4 antibody (A14435-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABHD4 antigen affinity purified polyclonal antibody (Catalog # A14435-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABHD4 at approximately 39 kDa. The expected band size for ABHD4 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14435-1-abhd4-primary-antibodies-fcm-testing-2_1.jpgAnti-ABHD4 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-ABHD4 antibody (A14435-1). Overlay histogram showing HEL cells stained with A14435-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABHD4 Antibody (A14435-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-abra-picoband-trade-antibody-a11558-1-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11558-1-abra-primary-antibodies-wb-testing-1.jpgAnti-ABRA Antibody Picoband® Western blot analysis of ABRA using anti-ABRA antibody (A11558-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABRA antigen affinity purified polyclonal antibody (Catalog # A11558-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABRA at approximately 39 kDa. The expected band size for ABRA is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-acid-phosphatase-acp1-picoband-trade-antibody-a02167-1-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02167-1-acp1-primary-antibodies-wb-testing-1.jpgAnti-Acid phosphatase/ACP1 Antibody Picoband® Western blot analysis of Acid phosphatase/ACP1 using anti-Acid phosphatase/ACP1 antibody (A02167-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hele whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse 4T1 whole cell lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid phosphatase/ACP1 antigen affinity purified polyclonal antibody (Catalog # A02167-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Acid phosphatase/ACP1 at approximately 17 kDa. The expected band size for Acid phosphatase/ACP1 is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-adam17-picoband-trade-antibody-a00604-4-boster.html2026-03-17T05:13:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00604-4-adam17-primary-antibodies-wb-testing-1.jpgAnti-Adam17 Antibody Picoband® Western blot analysis of Adam17 using anti-Adam17 antibody (A00604-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adam17 antigen affinity purified polyclonal antibody (Catalog # A00604-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adam17 at approximately 70 kDa. The expected band size for Adam17 is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-alpha-1-fetoprotein-afp-picoband-trade-antibody-a00522-3-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00522-3-afp-primary-antibodies-wb-testing-1.jpgAnti-alpha 1 Fetoprotein/AFP Antibody Picoband® Western blot analysis of alpha 1 Fetoprotein/AFP using anti-alpha 1 Fetoprotein/AFP antibody (A00522-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 1 Fetoprotein/AFP antigen affinity purified polyclonal antibody (Catalog # A00522-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 1 Fetoprotein/AFP at approximately 69 kDa. The expected band size for alpha 1 Fetoprotein/AFP is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00522-3-41598_2019_43039_fig5_html.pngAnti-alpha 1 Fetoprotein/AFP Antibody Picoband®Application of the optimised immunostaining and lectin staining methods. ( a ) CFTR levels in HT-29 cells. ( b ) HIF-1α levels in HEK-293T cells. ( c ) GAPDH levels in liver tissue of mouse. Various amounts (quantity represented in μg) of total cellular proteins analysed using 8% SDS-PAGE and immunostained using PVDF membrane and treated with or without fixation treatments. ( d ) AFP levels in the sera of healthy volunteers (n = 6) and HCC patients (n = 7), with different sample volumes using the PVDF membranes, with or without the fixation. ** Significantly different p < 0.01, *** p < 0.001, **** p < 0.0001. All values are means ± S.E. (error bars). ( e ) AAL and PHA-E staining, using 6 μg of proteins from the sera of healthy volunteers (n = 6) and prostate cancer patients (PC, n = 10), blotted on PVDF membranes, with or without fixation. Three representative healthy samples (lane i, ii, iii) and seven representative prostate cancer samples (lane iv-x) are presented (left). Boxplot provides the quantification of the total band intensities (right). Circle, healthy subjects; square, prostate cancer patients. Student’s t-test. ** P < 0.01 and **** P < 0.0001, healthy subjects vs. PC patients; # P < 0.0001, No fixation vs fixation groups. Band intensities were compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-019-43039-3'>31040299</a> https://www.bosterbio.com/products/primary-antibodies/anti-alyref-picoband-trade-antibody-a03580-3-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03580-3-alyref-primary-antibodies-wb-testing-1.jpgAnti-ALYREF Antibody Picoband® Western blot analysis of ALYREF using anti-ALYREF antibody (A03580-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: rat NRK whole cell lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse liver tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates, Lane 15: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALYREF antigen affinity purified polyclonal antibody (Catalog # A03580-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALYREF at approximately 35 kDa. The expected band size for ALYREF is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03580-3-alyref-primary-antibodies-ihc-testing-1.jpgAnti-ALYREF Antibody Picoband®IHC analysis of ALYREF using anti-ALYREF antibody (A03580-3). ALYREF was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALYREF Antibody (A03580-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03580-3-alyref-primary-antibodies-ihc-testing-2.jpgAnti-ALYREF Antibody Picoband®IHC analysis of ALY/ALYREF using anti-ALY/ALYREF antibody (A03580-3). ALY/ALYREF was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALY/ALYREF Antibody (A03580-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03580-3-alyref-primary-antibodies-ihc-testing-3.jpgAnti-ALYREF Antibody Picoband®IHC analysis of ALY/ALYREF using anti-ALY/ALYREF antibody (A03580-3). ALY/ALYREF was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALY/ALYREF Antibody (A03580-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03580-3-alyref-primary-antibodies-if-testing-2.jpgAnti-ALYREF Antibody Picoband® IF analysis of ALYREF using anti-ALYREF antibody (A03580-3). ALYREF was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALYREF Antibody (A03580-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03580-3-alyref-primary-antibodies-fcm-testing-3.jpgAnti-ALYREF Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ALYREF antibody (A03580-3). Overlay histogram showing SiHa cells stained with A03580-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALYREF Antibody (A03580-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-angiomotin-amot-picoband-trade-antibody-a04170-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04170-1-amot-primary-antibodies-wb-testing-1_1.jpgAnti-Angiomotin/AMOT Antibody Picoband® Western blot analysis of Angiomotin/AMOT using anti-Angiomotin/AMOT antibody (A04170-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiomotin/AMOT antigen affinity purified polyclonal antibody (Catalog # A04170-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiomotin/AMOT at approximately 140 kDa. The expected band size for Angiomotin/AMOT is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04170-1-amot-primary-antibodies-if-testing-2.jpgAnti-Angiomotin/AMOT Antibody Picoband® IF analysis of Angiomotin/AMOT using anti-Angiomotin/AMOT antibody (A04170-1). Angiomotin/AMOT was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Angiomotin/AMOT Antibody (A04170-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04170-1-amot-primary-antibodies-fcm-testing-3_1.jpgAnti-Angiomotin/AMOT Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-Angiomotin/AMOT antibody (A04170-1). Overlay histogram showing CACO-2 cells stained with A04170-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Angiomotin/AMOT Antibody (A04170-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-graf-arhgap26-picoband-trade-antibody-a08027-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08027-1-arhgap26-primary-antibodies-wb-testing-1.jpgAnti-GRAF/ARHGAP26 Antibody Picoband® Western blot analysis of GRAF/ARHGAP26 using anti-GRAF/ARHGAP26 antibody (A08027-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRAF/ARHGAP26 antigen affinity purified polyclonal antibody (Catalog # A08027-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRAF/ARHGAP26 at approximately 100 kDa. The expected band size for GRAF/ARHGAP26 is at 92 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-arhgap45-picoband-trade-antibody-a31863-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31863-1-arhgap45-primary-antibodies-wb-testing-1.jpgAnti-ARHGAP45 Antibody Picoband® Western blot analysis of ARHGAP45 using anti-ARHGAP45 antibody (A31863-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGAP45 antigen affinity purified polyclonal antibody (Catalog # A31863-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGAP45 at approximately 160 kDa. The expected band size for ARHGAP45 is at 125 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31863-1-arhgap45-primary-antibodies-ihc-testing-2.jpgAnti-ARHGAP45 Antibody Picoband® IHC analysis of ARHGAP45 using anti-ARHGAP45 antibody (A31863-1). ARHGAP45 was detected in a paraffin-embedded section of human B lymphocytic tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARHGAP45 Antibody (A31863-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31863-1-arhgap45-primary-antibodies-fcm-testing-3.jpgAnti-ARHGAP45 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ARHGAP45 antibody (A31863-1). Overlay histogram showing SiHa cells stained with A31863-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHGAP45 Antibody (A31863-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atp4a-picoband-trade-antibody-a08198-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08198-1-atp4a-primary-antibodies-wb-testing-1.jpgAnti-ATP4A Antibody Picoband® Western blot analysis of ATP4A using anti-ATP4A antibody (A08198-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP4A antigen affinity purified polyclonal antibody (Catalog # A08198-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP4A at approximately 130 kDa. The expected band size for ATP4A is at 114 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/n/eno1_a01250-3_human_cerebellum_16.38x.jpgAnti-ATP4A Antibody Picoband®IHC analysis of ATP4A using anti-ATP4A antibody (A08198-1). ATP4A was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP4A Antibody (A08198-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08198-1-atp4a-primary-antibodies-ihc-testing-3.jpgAnti-ATP4A Antibody Picoband®IHC analysis of ATP4A using anti-ATP4A antibody (A08198-1). ATP4A was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP4A Antibody (A08198-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08198-1-atp4a-primary-antibodies-ihc-testing-2.jpgAnti-ATP4A Antibody Picoband® IHC analysis of ATP4A using anti-ATP4A antibody (A08198-1). ATP4A was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP4A Antibody (A08198-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08198-1-atp4a-primary-antibodies-fcm-testing-3.jpgAnti-ATP4A Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-ATP4A antibody (A08198-1). Overlay histogram showing Daudi cells stained with A08198-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP4A Antibody (A08198-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-axin-2-axin2-picoband-trade-antibody-a01772-2-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01772-2-axin2-primary-antibodies-wb-testing-1.jpgAnti-Axin 2/AXIN2 Antibody Picoband® Western blot analysis of Axin 2/AXIN2 using anti-Axin 2/AXIN2 antibody (A01772-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Axin 2/AXIN2 antigen affinity purified polyclonal antibody (Catalog # A01772-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Axin 2/AXIN2 at approximately 100 kDa. The expected band size for Axin 2/AXIN2 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01772-2-axin2-primary-antibodies-ihc-testing-2.jpgAnti-Axin 2/AXIN2 Antibody Picoband® IHC analysis of Axin 2/AXIN2 using anti-Axin 2/AXIN2 antibody (A01772-2). Axin 2/AXIN2 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Axin 2/AXIN2 Antibody (A01772-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01772-2-axin2-primary-antibodies-ihc-testing-3.jpgAnti-Axin 2/AXIN2 Antibody Picoband® IHC analysis of Axin 2/AXIN2 using anti-Axin 2/AXIN2 antibody (A01772-2). Axin 2/AXIN2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Axin 2/AXIN2 Antibody (A01772-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01772-2-axin2-primary-antibodies-ihc-testing-4.jpgAnti-Axin 2/AXIN2 Antibody Picoband® IHC analysis of Axin 2/AXIN2 using anti-Axin 2/AXIN2 antibody (A01772-2). Axin 2/AXIN2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Axin 2/AXIN2 Antibody (A01772-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01772-2-axin2-primary-antibodies-fcm-testing-5.jpgAnti-Axin 2/AXIN2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Axin 2/AXIN2 antibody (A01772-2). Overlay histogram showing HepG2 cells stained with A01772-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Axin 2/AXIN2 Antibody (A01772-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bbs9-picoband-trade-antibody-a07367-2-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-wb-testing-1.jpgAnti-BBS9 Antibody Picoband® Western blot analysis of BBS9 using anti-BBS9 antibody (A07367-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBS9 antigen affinity purified polyclonal antibody (Catalog # A07367-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BBS9 at approximately 99 kDa. The expected band size for BBS9 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-ihc-testing-8.jpgAnti-BBS9 Antibody Picoband®IHC analysis of BBS9 using anti-BBS9 antibody (A07367-2). BBS9 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BBS9 Antibody (A07367-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-ihc-testing-2.jpgAnti-BBS9 Antibody Picoband® IHC analysis of BBS9 using anti-BBS9 antibody (A07367-2). BBS9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BBS9 Antibody (A07367-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-ihc-testing-3.jpgAnti-BBS9 Antibody Picoband® IHC analysis of BBS9 using anti-BBS9 antibody (A07367-2). BBS9 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BBS9 Antibody (A07367-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-ihc-testing-4.jpgAnti-BBS9 Antibody Picoband® IHC analysis of BBS9 using anti-BBS9 antibody (A07367-2). BBS9 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BBS9 Antibody (A07367-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-ihc-testing-5.jpgAnti-BBS9 Antibody Picoband® IHC analysis of BBS9 using anti-BBS9 antibody (A07367-2). BBS9 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BBS9 Antibody (A07367-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-ihc-testing-6.jpgAnti-BBS9 Antibody Picoband® IHC analysis of BBS9 using anti-BBS9 antibody (A07367-2). BBS9 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BBS9 Antibody (A07367-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-ihc-testing-7.jpgAnti-BBS9 Antibody Picoband® IHC analysis of BBS9 using anti-BBS9 antibody (A07367-2). BBS9 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BBS9 Antibody (A07367-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07367-2-bbs9-primary-antibodies-fcm-testing-8.jpgAnti-BBS9 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-BBS9 antibody (A07367-2). Overlay histogram showing RT4 cells stained with A07367-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BBS9 Antibody (A07367-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bcat1-picoband-trade-antibody-a05089-2-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05089-2-bcat1-primary-antibodies-wb-testing-1.jpgAnti-BCAT1 Antibody Picoband® Western blot analysis of BCAT1 using anti-BCAT1 antibody (A05089-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT1 antigen affinity purified polyclonal antibody (Catalog # A05089-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAT1 at approximately 43 kDa. The expected band size for BCAT1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05089-2-bcat1-primary-antibodies-ihc-testing-2.jpgAnti-BCAT1 Antibody Picoband® IHC analysis of BCAT1 using anti-BCAT1 antibody (A05089-2). BCAT1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (A05089-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05089-2-bcat1-primary-antibodies-ihc-testing-3.jpgAnti-BCAT1 Antibody Picoband® IHC analysis of BCAT1 using anti-BCAT1 antibody (A05089-2). BCAT1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (A05089-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05089-2-bcat1-primary-antibodies-ihc-testing-4.jpgAnti-BCAT1 Antibody Picoband® IHC analysis of BCAT1 using anti-BCAT1 antibody (A05089-2). BCAT1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (A05089-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05089-2-bcat1-primary-antibodies-fcm-testing-5_1.jpgAnti-BCAT1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-BCAT1 antibody (A05089-2). Overlay histogram showing U937 cells stained with A05089-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCAT1 Antibody (A05089-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-brd9-picoband-trade-antibody-a08420-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08420-1-brd9-primary-antibodies-wb-testing-1.jpgAnti-BRD9 Antibody Picoband® Western blot analysis of BRD9 using anti-BRD9 antibody (A08420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD9 antigen affinity purified polyclonal antibody (Catalog # A08420-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD9 at approximately 80 kDa. The expected band size for BRD9 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08420-1-brd9-primary-antibodies-fcm-testing-2.jpgAnti-BRD9 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-BRD9 antibody (A08420-1). Overlay histogram showing PC-3 cells stained with A08420-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRD9 Antibody (A08420-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-brdt-picoband-trade-antibody-a04985-2-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04985-2-brdt-primary-antibodies-wb-testing-1.jpgAnti-BRDT Antibody Picoband® Western blot analysis of BRDT using anti-BRDT antibody (A04985-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRDT antigen affinity purified polyclonal antibody (Catalog # A04985-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRDT at approximately 180 kDa. The expected band size for BRDT is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04985-2-brdt-primary-antibodies-fcm-testing-2.jpgAnti-BRDT Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-BRDT antibody (A04985-2). Overlay histogram showing Hela cells stained with A04985-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRDT Antibody (A04985-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bscl2-seipin-picoband-trade-antibody-a02753-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02753-1-bcat1-primary-antibodies-wb-testing-1.jpgAnti-BSCL2/Seipin Antibody Picoband® Western blot analysis of BSCL2/Seipin using anti-BSCL2/Seipin antibody (A02753-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BSCL2/Seipin antigen affinity purified polyclonal antibody (Catalog # A02753-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BSCL2/Seipin at approximately 46 kDa. The expected band size for BSCL2/Seipin is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-bst2-tetherin-picoband-trade-antibody-a00934-3-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00934-3-bst2-primary-antibodies-wb-testing-1_1.jpgAnti-BST2/Tetherin Antibody Picoband®Western blot analysis of BST2/Tetherin using anti-BST2/Tetherin antibody (A00934-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BST2/Tetherin antigen affinity purified polyclonal antibody (A00934-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BST2/Tetherin at approximately 28-37 kDa. The expected band size for BST2/Tetherin is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00934-3-bst2-primary-antibodies-ihc-testing-1.jpgAnti-BST2/Tetherin Antibody Picoband®IHC analysis of BST2/Tetherin using anti-BST2/Tetherin antibody (A00934-3). BST2/Tetherin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BST2/Tetherin Antibody (A00934-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00934-3-bst2-primary-antibodies-ihc-testing-2.jpgAnti-BST2/Tetherin Antibody Picoband®IHC analysis of BST2/Tetherin using anti-BST2/Tetherin antibody (A00934-3). BST2/Tetherin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BST2/Tetherin Antibody (A00934-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ccdc115-picoband-trade-antibody-a13807-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13807-1-ccdc115-primary-antibodies-wb-testing-1.jpgAnti-CCDC115 Antibody Picoband® Western blot analysis of CCDC115 using anti-CCDC115 antibody (A13807-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC115 antigen affinity purified polyclonal antibody (Catalog # A13807-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC115 at approximately 20 kDa. The expected band size for CCDC115 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13807-1-ccdc115-primary-antibodies-ihc-testing-2.jpgAnti-CCDC115 Antibody Picoband® IHC analysis of CCDC115 using anti-CCDC115 antibody (A13807-1). CCDC115 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCDC115 Antibody (A13807-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13807-1-ccdc115-primary-antibodies-ihc-testing-3.jpgAnti-CCDC115 Antibody Picoband® IHC analysis of CCDC115 using anti-CCDC115 antibody (A13807-1). CCDC115 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCDC115 Antibody (A13807-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13807-1-ccdc115-primary-antibodies-ihc-testing-4.jpgAnti-CCDC115 Antibody Picoband® IF analysis of CCDC115 using anti-CCDC115 antibody (A13807-1). CCDC115 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CCDC115 Antibody (A13807-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13807-1-ccdc115-primary-antibodies-fcm-testing-5.jpgAnti-CCDC115 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CCDC115 antibody (A13807-1). Overlay histogram showing U937 cells stained with A13807-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCDC115 Antibody (A13807-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cdc25b-picoband-trade-antibody-a01899-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01899-1-cdc25b-primary-antibodies-wb-testing-1.jpgAnti-CDC25B Antibody Picoband® Western blot analysis of CDC25B using anti-CDC25B antibody (A01899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human Hacat whole cell lysates. Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25B antigen affinity purified polyclonal antibody (Catalog # A01899-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25B at approximately 70 kDa. The expected band size for CDC25B is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01899-1-cdc25b-primary-antibodies-wb-testing-2.jpgAnti-CDC25B Antibody Picoband® Western blot analysis of CDC25B using anti-CDC25B antibody (A01899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25B antigen affinity purified polyclonal antibody (Catalog # A01899-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC25B at approximately 70 kDa. The expected band size for CDC25B is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01899-1-cdc25b-primary-antibodies-fcm-testing-3.jpgAnti-CDC25B Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-CDC25B antibody (A01899-1). Overlay histogram showing U87 cells stained with A01899-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC25B Antibody (A01899-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cep250-picoband-trade-antibody-a07150-1-boster.html2026-03-17T05:13:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07150-1-cep250-primary-antibodies-wb-testing-1.jpgAnti-CEP250 Antibody Picoband® Western blot analysis of CEP250 using anti-CEP250 antibody (A07150-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP250 antigen affinity purified polyclonal antibody (Catalog # A07150-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP250 at approximately 250 kDa. The expected band size for CEP250 is at 281 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07150-1-cep250-primary-antibodies-ihc-testing-2.jpgAnti-CEP250 Antibody Picoband® IHC analysis of CEP250 using anti-CEP250 antibody (A07150-1). CEP250 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CEP250 Antibody (A07150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07150-1-cep250-primary-antibodies-ihc-testing-3.jpgAnti-CEP250 Antibody Picoband® IHC analysis of CEP250 using anti-CEP250 antibody (A07150-1). CEP250 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CEP250 Antibody (A07150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07150-1-cep250-primary-antibodies-ihc-testing-4.jpgAnti-CEP250 Antibody Picoband® IHC analysis of CEP250 using anti-CEP250 antibody (A07150-1). CEP250 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CEP250 Antibody (A07150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07150-1-cep250-primary-antibodies-ihc-testing-5.jpgAnti-CEP250 Antibody Picoband® IHC analysis of CEP250 using anti-CEP250 antibody (A07150-1). CEP250 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CEP250 Antibody (A07150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07150-1-cep250-primary-antibodies-ihc-testing-6.jpgAnti-CEP250 Antibody Picoband® IHC analysis of CEP250 using anti-CEP250 antibody (A07150-1). CEP250 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CEP250 Antibody (A07150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07150-1-cep250-primary-antibodies-fcm-testing-7.jpgAnti-CEP250 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CEP250 antibody (A07150-1). Overlay histogram showing SiHa cells stained with A07150-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEP250 Antibody (A07150-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ces2-picoband-trade-antibody-a02868-2-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02868-2-ces2-primary-antibodies-wb-testing-1.jpgAnti-CES2 Antibody Picoband® Western blot analysis of CES2 using anti-CES2 antibody (A02868-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CES2 antigen affinity purified polyclonal antibody (Catalog # A02868-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CES2 at approximately 62 kDa. The expected band size for CES2 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02868-2-ces2-primary-antibodies-ihc-testing-2.jpgAnti-CES2 Antibody Picoband® IHC analysis of CES2 using anti-CES2 antibody (A02868-2). CES2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CES2 Antibody (A02868-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02868-2-ces2-primary-antibodies-if-testing-3.jpgAnti-CES2 Antibody Picoband® IF analysis of CES2 using anti-CES2 antibody (A02868-2). CES2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CES2 Antibody (A02868-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-c1orf77-fop-chtop-picoband-trade-antibody-a07770-2-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-wb-testing-1.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® Western blot analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse spleen tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1orf77/FOP/CHTOP antigen affinity purified polyclonal antibody (Catalog # A07770-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1orf77/FOP/CHTOP at approximately 28 kDa. The expected band size for C1orf77/FOP/CHTOP is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-ihc-testing-2.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-ihc-testing-3.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-ihc-testing-4.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-ihc-testing-5.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-ihc-testing-6.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-fcm-testing-8.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-C1orf77/FOP/CHTOP antibody (A07770-2). Overlay histogram showing THP-1 cells stained with A07770-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-ihc-testing-7.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® IHC analysis of C1orf77/FOP/CHTOP using anti-C1orf77/FOP/CHTOP antibody (A07770-2). C1orf77/FOP/CHTOP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07770-2-chtop-primary-antibodies-fcm-testing-9.jpgAnti-C1orf77/FOP/CHTOP Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-C1orf77/FOP/CHTOP antibody (A07770-2). Overlay histogram showing RH35 cells stained with A07770-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C1orf77/FOP/CHTOP Antibody (A07770-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-clusterin-clu-picoband-trade-antibody-a00590-3-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00590-3-clu-primary-antibodies-wb-testing-1.jpgAnti-Clusterin/CLU Antibody Picoband® Western blot analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Clusterin/CLU antigen affinity purified polyclonal antibody (Catalog # A00590-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Clusterin/CLU at approximately 70 kDa. The expected band size for Clusterin/CLU is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00590-3-clu-primary-antibodies-ihc-testing-4.jpgAnti-Clusterin/CLU Antibody Picoband®IHC analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Clusterin/CLU was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Clusterin/CLU Antibody (A00590-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00590-3-clu-primary-antibodies-ihc-testing-5.jpgAnti-Clusterin/CLU Antibody Picoband®IHC analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Clusterin/CLU was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Clusterin/CLU Antibody (A00590-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00590-3-clu-primary-antibodies-ihc-testing-2.jpgAnti-Clusterin/CLU Antibody Picoband® IHC analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Clusterin/CLU was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Clusterin/CLU Antibody (A00590-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00590-3-clu-primary-antibodies-ihc-testing-3.jpgAnti-Clusterin/CLU Antibody Picoband® IHC analysis of Clusterin/CLU using anti-Clusterin/CLU antibody (A00590-3). Clusterin/CLU was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Clusterin/CLU Antibody (A00590-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-crabp2-picoband-trade-antibody-a03297-3-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03297-3-crabp2-primary-antibodies-wb-testing-1.jpgAnti-CRABP2 Antibody Picoband® Western blot analysis of CRABP2 using anti-CRABP2 antibody (A03297-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRABP2 antigen affinity purified polyclonal antibody (Catalog # A03297-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRABP2 at approximately 17 kDa. The expected band size for CRABP2 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03297-3-crabp2-primary-antibodies-ihc-testing-2.jpgAnti-CRABP2 Antibody Picoband® IHC analysis of CRABP2 using anti-CRABP2 antibody (A03297-3). CRABP2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CRABP2 Antibody (A03297-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03297-3-crabp2-primary-antibodies-fcm-testing-3.jpgAnti-CRABP2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-CRABP2 antibody (A03297-3). Overlay histogram showing U251 cells stained with A03297-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRABP2 Antibody (A03297-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cxcr5-picoband-trade-antibody-a00663-2-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00663-2-cxcr5-primary-antibodies-wb-testing-1.jpgAnti-CXCR5 Antibody Picoband® Western blot analysis of CXCR5 using anti-CXCR5 antibody (A00663-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR5 antigen affinity purified polyclonal antibody (Catalog # A00663-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR5 at approximately 50 kDa. The expected band size for CXCR5 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00663-2-cxcr5-primary-antibodies-fcm-testing-2.jpgAnti-CXCR5 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-CXCR5 antibody (A00663-2). Overlay histogram showing HEL cells stained with A00663-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR5 Antibody (A00663-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dynamin-2-dnm2-picoband-trade-antibody-a01629-3-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01629-3-dnm2-primary-antibodies-wb-testing-1.jpgAnti-Dynamin 2/DNM2 Antibody Picoband® Western blot analysis of Dynamin 2/DNM2 using anti-Dynamin 2/DNM2 antibody (A01629-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dynamin 2/DNM2 antigen affinity purified polyclonal antibody (Catalog # A01629-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dynamin 2/DNM2 at approximately 100 kDa. The expected band size for Dynamin 2/DNM2 is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01629-3-dnm2-primary-antibodies-fcm-testing-2.jpgAnti-Dynamin 2/DNM2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-Dynamin 2/DNM2 antibody (A01629-3). Overlay histogram showing U937 cells stained with A01629-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dynamin 2/DNM2 Antibody (A01629-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dnmt3b-picoband-trade-antibody-a00319-5-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00319-5-dnmt3b-primary-antibodies-wb-testing-1.jpgAnti-DNMT3B Antibody Picoband® Western blot analysis of DNMT3B using anti-DNMT3B antibody (A00319-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3B antigen affinity purified polyclonal antibody (Catalog # A00319-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3B at approximately 100 kDa. The expected band size for DNMT3B is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00319-5-dnmt3b-primary-antibodies-fcm-testing-2.jpgAnti-DNMT3B Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-DNMT3B antibody (A00319-5). Overlay histogram showing CACO-2 cells stained with A00319-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT3B Antibody (A00319-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00319-5-dnmt3b-primary-antibodies-fcm-testing-3_1.jpgAnti-DNMT3B Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-DNMT3B antibody (A00319-5). Overlay histogram showing C6 cells stained with A00319-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT3B Antibody (A00319-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-e2f5-picoband-trade-antibody-a05265-1-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05265-1-e2f5-primary-antibodies-wb-testing-1.jpgAnti-E2F5 Antibody Picoband® Western blot analysis of E2F5 using anti-E2F5 antibody (A05265-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: huamn T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F5 antigen affinity purified polyclonal antibody (Catalog # A05265-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F5 at approximately 45 kDa. The expected band size for E2F5 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05265-1-e2f5-primary-antibodies-fcm-testing-2.jpgAnti-E2F5 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-E2F5 antibody (A05265-1). Overlay histogram showing JK cells stained with A05265-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-E2F5 Antibody (A05265-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eed-picoband-trade-antibody-a01345-2-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01345-2-eed-primary-antibodies-wb-testing-1.jpgAnti-EED Antibody Picoband® Western blot analysis of EED using anti-EED antibody (A01345-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EED antigen affinity purified polyclonal antibody (Catalog # A01345-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EED at approximately 70 kDa. The expected band size for EED is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01345-2-eed-primary-antibodies-fcm-testing-2.jpgAnti-EED Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-EED antibody (A01345-2). Overlay histogram showing A549 cells stained with A01345-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EED Antibody (A01345-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-elk1-picoband-trade-antibody-a01426-1-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01426-1-elk1-primary-antibodies-wb-testing-1.jpgAnti-ELK1 Antibody Picoband® Western blot analysis of ELK1 using anti-ELK1 antibody (A01426-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELK1 antigen affinity purified polyclonal antibody (Catalog # A01426-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELK1 at approximately 50 kDa. The expected band size for ELK1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01426-1-elk1-primary-antibodies-fcm-testing-2.jpgAnti-ELK1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ELK1 antibody (A01426-1). Overlay histogram showing SiHa cells stained with A01426-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ELK1 Antibody (A01426-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-her2-erbb2-picoband-trade-antibody-a00010-5-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00010-5-erbb2-primary-antibodies-wb-testing-1.jpgAnti-HER2/ERBB2 Antibody Picoband® Western blot analysis of HER2/ERBB2 using anti-HER2/ERBB2 antibody (A00010-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HER2/ERBB2 antigen affinity purified polyclonal antibody (Catalog # A00010-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HER2/ERBB2 at approximately 185 kDa. The expected band size for HER2/ERBB2 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00010-5-erbb2-primary-antibodies-ihc-testing-2.jpgAnti-HER2/ERBB2 Antibody Picoband® IHC analysis of HER2/ERBB2 using anti-HER2/ERBB2 antibody (A00010-5). HER2/ERBB2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER2/ERBB2 Antibody (A00010-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00010-5-erbb2-primary-antibodies-fcm-testing-3.jpgAnti-HER2/ERBB2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-HER2/ERBB2 antibody (A00010-5). Overlay histogram showing 293T cells stained with A00010-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HER2/ERBB2 Antibody (A00010-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-faa-fah-picoband-trade-antibody-a02072-1-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02072-1-fah-primary-antibodies-wb-testing-1.jpgAnti-FAA/FAH Antibody Picoband® Western blot analysis of FAA/FAH using anti-FAA/FAH antibody (A02072-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAA/FAH antigen affinity purified polyclonal antibody (Catalog # A02072-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAA/FAH at approximately 41 kDa. The expected band size for FAA/FAH is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02072-1-fah-primary-antibodies-ihc-testing-2.jpgAnti-FAA/FAH Antibody Picoband® IHC analysis of FAA/FAH using anti-FAA/FAH antibody (A02072-1). FAA/FAH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAA/FAH Antibody (A02072-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02072-1-fah-primary-antibodies-ihc-testing-3.jpgAnti-FAA/FAH Antibody Picoband® IHC analysis of FAA/FAH using anti-FAA/FAH antibody (A02072-1). FAA/FAH was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAA/FAH Antibody (A02072-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02072-1-fah-primary-antibodies-ihc-testing-4.jpgAnti-FAA/FAH Antibody Picoband® IHC analysis of FAA/FAH using anti-FAA/FAH antibody (A02072-1). FAA/FAH was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAA/FAH Antibody (A02072-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02072-1-fah-primary-antibodies-fcm-testing-5.jpgAnti-FAA/FAH Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-FAA/FAH antibody (A02072-1). Overlay histogram showing PC-3 cells stained with A02072-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAA/FAH Antibody (A02072-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd64-fcgr1a-picoband-trade-antibody-a02428-3-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02428-3-fcgr1a-primary-antibodies-wb-testing-1.jpgAnti-CD64/FCGR1A Antibody Picoband® Western blot analysis of CD64/FCGR1A using anti-CD64/FCGR1A antibody (A02428-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse EL-4 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD64/FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD64/FCGR1A at approximately 70 kDa. The expected band size for CD64/FCGR1A is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02428-3-fcgr1a-primary-antibodies-fcm-testing-2_1.jpgAnti-CD64/FCGR1A Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-CD64/FCGR1A antibody (A02428-3). Overlay histogram showing SiHa cells stained with A02428-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD64/FCGR1A Antibody (A02428-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fanca-faa-picoband-trade-antibody-a03662-2-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03662-2-fanca-primary-antibodies-wb-testing-1.jpgAnti-Fanca/FAA Antibody Picoband® Western blot analysis of Fanca/FAA using anti-Fanca/FAA antibody (A03662-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fanca/FAA antigen affinity purified polyclonal antibody (Catalog # A03662-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fanca/FAA at approximately 180 kDa. The expected band size for Fanca/FAA is at 161 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03662-2-fanca-primary-antibodies-fcm-testing-2.jpgAnti-Fanca/FAA Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Fanca/FAA antibody (A03662-2). Overlay histogram showing ANA-1 cells stained with A03662-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fanca/FAA Antibody (A03662-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fgfr2-picoband-trade-antibody-a00231-2-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00231-2-fgfr2-primary-antibodies-wb-testing-1.jpgAnti-FGFR2 Antibody Picoband® Western blot analysis of FGFR2 using anti-FGFR2 antibody (A00231-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR2 antigen affinity purified polyclonal antibody (Catalog # A00231-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR2 at approximately 145 kDa. The expected band size for FGFR2 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00231-2-fgfr2-primary-antibodies-ihc-testing-2.jpgAnti-FGFR2 Antibody Picoband® IHC analysis of FGFR2 using anti-FGFR2 antibody (A00231-2). FGFR2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGFR2 Antibody (A00231-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00231-2-fgfr2-primary-antibodies-ihc-testing-3.jpgAnti-FGFR2 Antibody Picoband® IHC analysis of FGFR2 using anti-FGFR2 antibody (A00231-2). FGFR2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGFR2 Antibody (A00231-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00231-2-fgfr2-primary-antibodies-fcm-testing-4.jpgAnti-FGFR2 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-FGFR2 antibody (A00231-2). Overlay histogram showing Daudi cells stained with A00231-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FGFR2 Antibody (A00231-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fkbp1a-1b-picoband-trade-antibody-a04492-3-boster.html2026-03-17T05:13:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04492-3-fkbp1a-1b-primary-antibodies-wb-testing-1.jpgAnti-FKBP1A/1B Antibody Picoband® Western blot analysis of FKBP1A/1B using anti-FKBP1A/1B antibody (A04492-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: huamn MOLT-4 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP1A/1B antigen affinity purified polyclonal antibody (Catalog # A04492-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP1A/1B at approximately 15 kDa. The expected band size for FKBP1A/1B is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04492-3-fkbp1a-1b-primary-antibodies-ihc-testing-2.jpgAnti-FKBP1A/1B Antibody Picoband® IHC analysis of FKBP1A/1B using anti-FKBP1A/1B antibody (A04492-3). FKBP1A/1B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FKBP1A/1B Antibody (A04492-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04492-3-fkbp1a-1b-primary-antibodies-fcm-testing-3.jpgAnti-FKBP1A/1B Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-FKBP1A/1B antibody (A04492-3). Overlay histogram showing RT4 cells stained with A04492-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FKBP1A/1B Antibody (A04492-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04492-3-fkbp1a-1b-primary-antibodies-fcm-testing-4.jpgAnti-FKBP1A/1B Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-FKBP1A/1B antibody (A04492-3). Overlay histogram showing SiHa cells stained with A04492-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FKBP1A/1B Antibody (A04492-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fxr2-picoband-trade-antibody-a05130-4-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05130-4-fxr2-primary-antibodies-wb-testing-1.jpgAnti-FXR2 Antibody Picoband® Western blot analysis of FXR2 using anti-FXR2 antibody (A05130-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: huamn U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXR2 antigen affinity purified polyclonal antibody (Catalog # A05130-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXR2 at approximately 93 kDa. The expected band size for FXR2 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05130-4-fxr2-primary-antibodies-fcm-testing-2.jpgAnti-FXR2 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-FXR2 antibody (A05130-4). Overlay histogram showing HEPA1-6 cells stained with A05130-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FXR2 Antibody (A05130-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05130-4-fxr2-primary-antibodies-fcm-testing-3_1.jpgAnti-FXR2 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-FXR2 antibody (A05130-4). Overlay histogram showing RT4 cells stained with A05130-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FXR2 Antibody (A05130-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-glud1-2-picoband-trade-antibody-a01866-2-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-wb-testing-1.jpgAnti-GLUD1/2 Antibody Picoband® Western blot analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUD1/2 antigen affinity purified polyclonal antibody (Catalog # A01866-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUD1/2 at approximately 54 kDa. The expected band size for GLUD1/2 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-2.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-3.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-4.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-5.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-6.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-7.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-8.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human gallbladder carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-9.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-10.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-11.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-ihc-testing-12.jpgAnti-GLUD1/2 Antibody Picoband® IHC analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-if-testing-13.jpgAnti-GLUD1/2 Antibody Picoband® IF analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-fcm-testing-14_1.jpgAnti-GLUD1/2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-GLUD1/2 antibody (A01866-2). Overlay histogram showing SiHa cells stained with A01866-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLUD1/2 Antibody (A01866-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01866-2-glud1-2-primary-antibodies-if-testing-15.jpgAnti-GLUD1/2 Antibody Picoband® IF analysis of GLUD1/2 using anti-GLUD1/2 antibody (A01866-2). GLUD1/2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GLUD1/2 Antibody (A01866-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-golga3-picoband-trade-antibody-a07522-2-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07522-2-golga3-primary-antibodies-wb-testing-1.jpgAnti-GOLGA3 Antibody Picoband® Western blot analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLGA3 antigen affinity purified polyclonal antibody (Catalog # A07522-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLGA3 at approximately 180 kDa. The expected band size for GOLGA3 is at 167 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07522-2-golga3-primary-antibodies-ihc-testing-2.jpgAnti-GOLGA3 Antibody Picoband® IHC analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2). GOLGA3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA3 Antibody (A07522-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07522-2-golga3-primary-antibodies-ihc-testing-3.jpgAnti-GOLGA3 Antibody Picoband® IHC analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2). GOLGA3 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA3 Antibody (A07522-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07522-2-golga3-primary-antibodies-if-testing-4.jpgAnti-GOLGA3 Antibody Picoband® IF analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2). GOLGA3 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GOLGA3 Antibody (A07522-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-gpr50-picoband-trade-antibody-a07827-2-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07827-2-gpr50-primary-antibodies-wb-testing-1.jpgAnti-GPR50 Antibody Picoband® Western blot analysis of GPR50 using anti-GPR50 antibody (A07827-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR50 antigen affinity purified polyclonal antibody (Catalog # A07827-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR50 at approximately 67 kDa. The expected band size for GPR50 is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-gpr161-picoband-trade-antibody-a10567-1-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10567-1-gpr161-primary-antibodies-wb-testing-1.jpgAnti-GPR161 Antibody Picoband® Western blot analysis of GPR161 using anti-GPR161 antibody (A10567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: huamn SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR161 antigen affinity purified polyclonal antibody (Catalog # A10567-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR161 at approximately 60 kDa. The expected band size for GPR161 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-grasp1-gripap1-picoband-trade-antibody-a13005-2-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13005-2-gripap1-primary-antibodies-wb-testing-1.jpgAnti-GRASP1/GRIPAP1 Antibody Picoband® Western blot analysis of GRASP1/GRIPAP1 using anti-GRASP1/GRIPAP1 antibody (A13005-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: huamn HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat panceras tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse pancreas tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRASP1/GRIPAP1 antigen affinity purified polyclonal antibody (Catalog # A13005-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRASP1/GRIPAP1 at approximately 96 kDa. The expected band size for GRASP1/GRIPAP1 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13005-2-gripap1-primary-antibodies-fcm-testing-2.jpgAnti-GRASP1/GRIPAP1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-GRASP1/GRIPAP1 antibody (A13005-2). Overlay histogram showing HepG2 cells stained with A13005-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRASP1/GRIPAP1 Antibody (A13005-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hcls1-picoband-trade-antibody-a04313-1-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04313-1-hcls1-primary-antibodies-wb-testing-1.jpgAnti-HCLS1 Antibody Picoband® Western blot analysis of HCLS1 using anti-HCLS1 antibody (A04313-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCLS1 antigen affinity purified polyclonal antibody (Catalog # A04313-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCLS1 at approximately 75 kDa. The expected band size for HCLS1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04313-1-hcls1-primary-antibodies-wb-testing-2.jpgAnti-HCLS1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-HCLS1 antibody (A04313-1). Overlay histogram showing RT4 cells stained with A04313-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HCLS1 Antibody (A04313-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-irs2-picoband-trade-antibody-a00805-2-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-2-irs2-primary-antibodies-wb-testing-1.jpgAnti-IRS2 Antibody Picoband® Western blot analysis of IRS2 using anti-IRS2 antibody (A00805-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRS2 antigen affinity purified polyclonal antibody (Catalog # A00805-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRS2 at approximately 180 kDa. The expected band size for IRS2 is at 137 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-2-irs2-primary-antibodies-ihc-testing-2.jpgAnti-IRS2 Antibody Picoband® IHC analysis of IRS2 using anti-IRS2 antibody (A00805-2). IRS2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRS2 Antibody (A00805-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-2-irs2-primary-antibodies-if-testing-3.jpgAnti-IRS2 Antibody Picoband® IF analysis of IRS2 using anti-IRS2 antibody (A00805-2). IRS2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IRS2 Antibody (A00805-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-2-irs2-primary-antibodies-fcm-testing-4.jpgAnti-IRS2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-IRS2 antibody (A00805-2). Overlay histogram showing 293T cells stained with A00805-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRS2 Antibody (A00805-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-irs2-picoband-trade-antibody-a00805-3-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-3-irs2-primary-antibodies-wb-testing-1.jpgAnti-IRS2 Antibody Picoband® Western blot analysis of IRS2 using anti-IRS2 antibody (A00805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRS2 antigen affinity purified polyclonal antibody (Catalog # A00805-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRS2 at approximately 180 kDa. The expected band size for IRS2 is at 137 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-3-irs2-primary-antibodies-ihc-testing-2.jpgAnti-IRS2 Antibody Picoband® IHC analysis of IRS2 using anti-IRS2 antibody (A00805-3). IRS2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRS2 Antibody (A00805-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-3-irs2-primary-antibodies-ihc-testing-3.jpgAnti-IRS2 Antibody Picoband® IHC analysis of IRS2 using anti-IRS2 antibody (A00805-3). IRS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRS2 Antibody (A00805-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00805-3-irs2-primary-antibodies-ihc-testing-4.jpgAnti-IRS2 Antibody Picoband® IHC analysis of IRS2 using anti-IRS2 antibody (A00805-3). IRS2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRS2 Antibody (A00805-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-integrin-beta-3-itgb3-picoband-trade-antibody-a00587-1-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00587-1-itgb3-primary-antibodies-wb-testing-1.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband® Western blot analysis of Integrin beta 3/ITGB3 using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 3/ITGB3 antigen affinity purified polyclonal antibody (Catalog # A00587-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin beta 3/ITGB3 at approximately 100 kDa. The expected band size for Integrin beta 3/ITGB3 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00587-1-itgb3-primary-antibodies-ihc-testing-2.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband® IHC analysis of Integrin beta 3/ITGB3 using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Integrin beta 3/ITGB3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Integrin beta 3/ITGB3 Antibody (A00587-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00587-1-itgb3-primary-antibodies-ihc-testing-3.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband® IHC analysis of Integrin beta 3/ITGB3 using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Integrin beta 3/ITGB3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Integrin beta 3/ITGB3 Antibody (A00587-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00587-1-itgb3-primary-antibodies-ihc-testing-4.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband® IHC analysis of Integrin beta 3/ITGB3 using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Integrin beta 3/ITGB3 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Integrin beta 3/ITGB3 Antibody (A00587-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00587-1-itgb3-primary-antibodies-fcm-testing-5_1.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-Integrin beta 3/ITGB3 antibody (A00587-1). Overlay histogram showing HEL cells stained with A00587-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin beta 3/ITGB3 Antibody (A00587-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-kmo-picoband-trade-antibody-a05469-3-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05469-3-kmo-primary-antibodies-wb-testing-1.jpgAnti-KMO Antibody Picoband® Western blot analysis of KMO using anti-KMO antibody (A05469-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KMO antigen affinity purified polyclonal antibody (Catalog # A05469-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KMO at approximately 50 kDa. The expected band size for KMO is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05469-3-kmo-primary-antibodies-ihc-testing-2.jpgAnti-KMO Antibody Picoband® IHC analysis of KMO using anti-KMO antibody (A05469-3). KMO was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KMO Antibody (A05469-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05469-3-kmo-primary-antibodies-ihc-testing-3.jpgAnti-KMO Antibody Picoband® IHC analysis of KMO using anti-KMO antibody (A05469-3). KMO was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KMO Antibody (A05469-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05469-3-kmo-primary-antibodies-ihc-testing-4.jpgAnti-KMO Antibody Picoband® IHC analysis of KMO using anti-KMO antibody (A05469-3). KMO was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KMO Antibody (A05469-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05469-3-kmo-primary-antibodies-fcm-testing-5.jpgAnti-KMO Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-KMO antibody (A05469-3). Overlay histogram showing Daudi cells stained with A05469-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-KMO Antibody (A05469-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05469-3-kmo-primary-antibodies-fcm-testing-6.jpgAnti-KMO Antibody Picoband® Flow Cytometry analysis of Neuro-2a cells using anti-KMO antibody (A05469-3). Overlay histogram showing Neuro-2a cells stained with A05469-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-KMO Antibody (A05469-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mcm8-picoband-trade-antibody-a06148-1-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06148-1-mcm8-primary-antibodies-wb-testing-1.jpgAnti-MCM8 Antibody Picoband® Western blot analysis of MCM8 using anti-MCM8 antibody (A06148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat H9C2(2-1) whole cell lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM8 antigen affinity purified polyclonal antibody (Catalog # A06148-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM8 at approximately 94 kDa. The expected band size for MCM8 is at 94 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-mff-picoband-trade-antibody-a02563-1-boster.html2026-03-17T05:13:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-wb-testing-1.jpgAnti-MFF Antibody Picoband® Western blot analysis of MFF using anti-MFF antibody (A02563-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human MOLT-4 whole clel lysates, Lane 5: human HEL whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFF antigen affinity purified polyclonal antibody (Catalog # A02563-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFF at approximately 26 kDa. The expected band size for MFF is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-ihc-testing-2.jpgAnti-MFF Antibody Picoband® IHC analysis of MFF using anti-MFF antibody (A02563-1). MFF was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFF Antibody (A02563-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-ihc-testing-3.jpgAnti-MFF Antibody Picoband® IHC analysis of MFF using anti-MFF antibody (A02563-1). MFF was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFF Antibody (A02563-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-ihc-testing-4.jpgAnti-MFF Antibody Picoband® IHC analysis of MFF using anti-MFF antibody (A02563-1). MFF was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFF Antibody (A02563-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-ihc-testing-5.jpgAnti-MFF Antibody Picoband® IHC analysis of MFF using anti-MFF antibody (A02563-1). MFF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFF Antibody (A02563-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-ihc-testing-6.jpgAnti-MFF Antibody Picoband® IHC analysis of MFF using anti-MFF antibody (A02563-1). MFF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFF Antibody (A02563-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-fcm-testing-8.jpgAnti-MFF Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-MFF antibody (A02563-1). Overlay histogram showing SiHa cells stained with A02563-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MFF Antibody (A02563-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-if-testing-9.jpgAnti-MFF Antibody Picoband® IF analysis of MFF using anti-MFF antibody (A02563-1). MFF was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MFF Antibody (A02563-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02563-1-mff-primary-antibodies-if-testing-7.jpgAnti-MFF Antibody Picoband® IF analysis of MFF using anti-MFF antibody (A02563-1). MFF was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MFF Antibody (A02563-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-mipol1-picoband-trade-antibody-a11968-1-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11968-1-mipol1-primary-antibodies-wb-testing-1.jpgAnti-MIPOL1 Antibody Picoband® Western blot analysis of MIPOL1 using anti-MIPOL1 antibody (A11968-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIPOL1 antigen affinity purified polyclonal antibody (Catalog # A11968-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIPOL1 at approximately 52 kDa. The expected band size for MIPOL1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11968-1-mipol1-primary-antibodies-fcm-testing-2.jpgAnti-MIPOL1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-MIPOL1 antibody (A11968-1). Overlay histogram showing HepG2 cells stained with A11968-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIPOL1 Antibody (A11968-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ki67-mki67-picoband-trade-antibody-a00254-1-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-ki67-primary-antibodies-ihc-testing-1.jpgAnti-Ki67/MKI67 Antibody IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1). Ki67/MKI67 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-oncotarget-06-31702-g003.jpgAnti-Ki67/MKI67 AntibodyKDM4B promotes AR-mediated carcinogenesis in MFE-296 cells. MFE-296 cells were transiently transfected with negative control (NC), AR (siAR) siRNA, KDM4B (siKDM4B) siRNA or KDM4B and AR siRNA together (siKDM4B+siAR). Cell proliferation was determined by MTT assay A. and colony formation assays B, C. Migrated and invasive MFE-296 cells on the lower surface of the Transwell filter were stained and photographed, 200×. The number of migrated and invasive cells is shown on the right. * P < 0.05, ** P < 0.01, *** P < 0.005 compared with the NC group. D. KDM4B knockdown efficiency in shKDM4B group was confirmed by qRT-PCR. The tumor weight E. and tumor volumes F. and G. formed from nude mice injected subcutaneously with MFE-296 cells stably transfected with NC (MFE-296/NC) or shKDM4B (MFE-296/shKDM4B) were shown. H. Staining with hematoxylin and eosin (H&E) or immunohistochemical staining for KDM4B, AR, c-myc, and ki-67 in mouse tumor tissues (400×). Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741634/'>26397136</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-oncotarget-06-31702-g006.jpgAnti-Ki67/MKI67 AntibodyKDM4A, but not KDM4B, promotes AR-mediated carcinogenesis in AN3CA cells. A. AN3CA cells were transiently transfected with negative control (NC), AR (siAR) siRNA, KDM4A (siKDM4A) siRNA or KDM4A and AR siRNA together (siKDM4A+siAR). Cell proliferation was determined by the MTT assay (A) and colony formation assays B, C. Migrated and invasive AN3CA cells on the lower surface of the Transwell filter were stained and photographed, 200×. The number of migrated and invasive cells is shown on the right. * P < 0.05, ** P < 0.01 compared with the NC group. D. KDM4A knockdown efficiency in shKDM4A group was confirmed by qRT-PCR. Tumor weight E. and tumor volumes F. and G. from nude mice injected subcutaneously with AN3CA cells stably transfected with NC (AN3CA/NC) or shKDM4A (AN3CA/shKDM4A). H. Staining with hematoxylin and eosin (H&E) or immunohistochemical staining for KDM4A, AR, p27 kip1 , ki-67 and H3k4me3 in mouse tumor tissues (400×). Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741634/'>26397136</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-12885_2013_article_4302_fig7_html.jpgAnti-Ki67/MKI67 AntibodyTumorigenicity assay in nude mice. A : The growth rates of tumors formed from untransfected MFE-296 cells (MFE-296) and MFE-296 cells transfected with NC (MFE-296/NC) or shFOXA1 (MFE-296/shFOXA1). After injection, tumor volumes were calculated every seven days. B and C : Six weeks after injection of MFE-296, MFE-296-NC, and MFE-296-shFOXA1 cells, tumors were removed, and the tumor weights and volumes were determined. Arithmetic means and SD are shown. D : Staining with hematoxylin and eosin (H&E) or immunohistochemical staining for FOXA1, AR, Notch1, Hes1, Ki67, and PCNA in tumor tissues (immunohistochemical staining, 200×). *p < 0.05 compared with the NC group. Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1471-2407-14-78'>24512546</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-ki67-primary-antibodies-ihc-testing-2.jpgAnti-Ki67/MKI67 Antibody IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1). Ki67/MKI67 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-ki67-primary-antibodies-ihc-testing-3.jpgAnti-Ki67/MKI67 Antibody IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1). Ki67/MKI67 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-ki67-primary-antibodies-ihc-testing-4.jpgAnti-Ki67/MKI67 Antibody IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1). Ki67/MKI67 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00254-1-ki67-primary-antibodies-ihc-testing-5.jpgAnti-Ki67/MKI67 Antibody IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (A00254-1). Ki67/MKI67 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/MKI67 Antibody (A00254-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-mre11-picoband-trade-antibody-a32234-1-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32234-1-mre11-primary-antibodies-wb-testing-1.jpgAnti-MRE11 Antibody Picoband® Western blot analysis of MRE11 using anti-MRE11 antibody (A32234-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human HEL whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat testis tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse testis tissue lysates, Lane 13: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRE11 antigen affinity purified polyclonal antibody (Catalog # A32234-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRE11 at approximately 93 kDa. The expected band size for MRE11 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32234-1-mre11-primary-antibodies-ihc-testing-2.jpgAnti-MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A32234-1). MRE11 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRE11 Antibody (A32234-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32234-1-mre11-primary-antibodies-ihc-testing-3.jpgAnti-MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A32234-1). MRE11 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRE11 Antibody (A32234-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32234-1-mre11-primary-antibodies-ihc-testing-4.jpgAnti-MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A32234-1). MRE11 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRE11 Antibody (A32234-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32234-1-mre11-primary-antibodies-ihc-testing-5.jpgAnti-MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A32234-1). MRE11 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRE11 Antibody (A32234-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32234-1-mre11-primary-antibodies-ihc-testing-6.jpgAnti-MRE11 Antibody Picoband® IHC analysis of MRE11 using anti-MRE11 antibody (A32234-1). MRE11 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRE11 Antibody (A32234-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32234-1-mre11-primary-antibodies-fcm-testing-7.jpgAnti-MRE11 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-MRE11 antibody (A32234-1). Overlay histogram showing HL-60 cells stained with A32234-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MRE11 Antibody (A32234-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mim-mtss1-picoband-trade-antibody-a04325-1-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04325-1-mtss1-primary-antibodies-wb-testing-1.jpgAnti-MIM/MTSS1 Antibody Picoband® Western blot analysis of MIM/MTSS1 using anti-MIM/MTSS1 antibody (A04325-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIM/MTSS1 antigen affinity purified polyclonal antibody (Catalog # A04325-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIM/MTSS1 at approximately 115 kDa. The expected band size for MIM/MTSS1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04325-1-mtss1-primary-antibodies-ihc-testing-2.jpgAnti-MIM/MTSS1 Antibody Picoband® IHC analysis of MIM/MTSS1 using anti-MIM/MTSS1 antibody (A04325-1). MIM/MTSS1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIM/MTSS1 Antibody (A04325-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04325-1-mtss1-primary-antibodies-ihc-testing-3.jpgAnti-MIM/MTSS1 Antibody Picoband® IHC analysis of MIM/MTSS1 using anti-MIM/MTSS1 antibody (A04325-1). MIM/MTSS1 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIM/MTSS1 Antibody (A04325-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04325-1-mtss1-primary-antibodies-ihc-testing-4.jpgAnti-MIM/MTSS1 Antibody Picoband® IHC analysis of MIM/MTSS1 using anti-MIM/MTSS1 antibody (A04325-1). MIM/MTSS1 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIM/MTSS1 Antibody (A04325-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04325-1-mtss1-primary-antibodies-ihc-testing-5.jpgAnti-MIM/MTSS1 Antibody Picoband® IHC analysis of MIM/MTSS1 using anti-MIM/MTSS1 antibody (A04325-1). MIM/MTSS1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIM/MTSS1 Antibody (A04325-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04325-1-mtss1-primary-antibodies-fcm-testing-6.jpgAnti-MIM/MTSS1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-MIM/MTSS1 antibody (A04325-1). Overlay histogram showing RT4 cells stained with A04325-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIM/MTSS1 Antibody (A04325-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mx1-picoband-trade-antibody-a00849-1-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00849-1-mx1-primary-antibodies-wb-testing-1.jpgAnti-MX1 Antibody Picoband® Western blot analysis of MX1 using anti-MX1 antibody (A00849-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MX1 antigen affinity purified polyclonal antibody (Catalog # A00849-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MX1 at approximately 76 kDa. The expected band size for MX1 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00849-1-mx1-primary-antibodies-fcm-testing-2.jpgAnti-MX1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-MX1 antibody (A00849-1). Overlay histogram showing RT4 cells stained with A00849-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MX1 Antibody (A00849-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ndfip1-picoband-trade-antibody-a04644-2-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04644-2-ndfip1-primary-antibodies-wb-testing-1.jpgAnti-NDFIP1 Antibody Picoband® Western blot analysis of NDFIP1 using anti-NDFIP1 antibody (A04644-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDFIP1 antigen affinity purified polyclonal antibody (Catalog # A04644-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDFIP1 at approximately 28 kDa. The expected band size for NDFIP1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04644-2-ndfip1-primary-antibodies-fcm-testing-2.jpgAnti-NDFIP1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-NDFIP1 antibody (A04644-2). Overlay histogram showing RT4 cells stained with A04644-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDFIP1 Antibody (A04644-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-capon-nos1ap-picoband-trade-antibody-a03060-3-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03060-3-nos1ap-primary-antibodies-wb-testing-1.jpgAnti-CAPON/NOS1AP Antibody Picoband® Western blot analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPON/NOS1AP antigen affinity purified polyclonal antibody (Catalog # A03060-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPON/NOS1AP at approximately 60 kDa. The expected band size for CAPON/NOS1AP is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03060-3-nos1ap-primary-antibodies-ihc-testing-4.jpgAnti-CAPON/NOS1AP Antibody Picoband®IHC analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3). CAPON/NOS1AP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAPON/NOS1AP Antibody (A03060-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03060-3-nos1ap-primary-antibodies-ihc-testing-2.jpgAnti-CAPON/NOS1AP Antibody Picoband® IHC analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3). CAPON/NOS1AP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAPON/NOS1AP Antibody (A03060-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03060-3-nos1ap-primary-antibodies-ihc-testing-3.jpgAnti-CAPON/NOS1AP Antibody Picoband® IHC analysis of CAPON/NOS1AP using anti-CAPON/NOS1AP antibody (A03060-3). CAPON/NOS1AP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAPON/NOS1AP Antibody (A03060-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03060-3-nos1ap-primary-antibodies-fcm-testing-4.jpgAnti-CAPON/NOS1AP Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-CAPON/NOS1AP antibody (A03060-3). Overlay histogram showing HEL cells stained with A03060-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CAPON/NOS1AP Antibody (A03060-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-olig2-picoband-trade-antibody-a02247-2-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02247-2-olig2-primary-antibodies-wb-testing-1.jpgAnti-OLIG2 Antibody Picoband® Western blot analysis of OLIG2 using anti-OLIG2 antibody (A02247-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLIG2 antigen affinity purified polyclonal antibody (Catalog # A02247-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLIG2 at approximately 40 kDa. The expected band size for OLIG2 is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-prkag2-picoband-trade-antibody-a02758-3-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02758-3-prkag2-primary-antibodies-wb-testing-1.jpgAnti-PRKAG2 Antibody Picoband® Western blot analysis of PRKAG2 using anti-PRKAG2 antibody (A02758-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAG2 antigen affinity purified polyclonal antibody (Catalog # A02758-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAG2 at approximately 63 kDa. The expected band size for PRKAG2 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02758-3-prkag2-primary-antibodies-fcm-testing-2.jpgAnti-PRKAG2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-PRKAG2 antibody (A02758-3). Overlay histogram showing PC-3 cells stained with A02758-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRKAG2 Antibody (A02758-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rad21-picoband-trade-antibody-a01864-2-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01864-2-rad21-primary-antibodies-wb-testing-1_1.jpgAnti-RAD21 Antibody Picoband®Western blot analysis of RAD21 using anti-RAD21 antibody (A01864-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT4 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 antigen affinity purified polyclonal antibody (A01864-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAD21 at approximately 130 kDa. The expected band size for RAD21 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01864-2-rad21-primary-antibodies-if-testing-2.jpgAnti-RAD21 Antibody Picoband® IF analysis of RAD21 using anti-RAD21 antibody (A01864-2). RAD21 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAD21 Antibody (A01864-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01864-2-rad21-primary-antibodies-fcm-testing-3.jpgAnti-RAD21 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RAD21 antibody (A01864-2). Overlay histogram showing HL-60 cells stained with A01864-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD21 Antibody (A01864-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnf8-picoband-trade-antibody-a00707-3-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00707-3-rnf8-primary-antibodies-wb-testing-1.jpgAnti-RNF8 Antibody Picoband® Western blot analysis of RNF8 using anti-RNF8 antibody (A00707-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF8 antigen affinity purified polyclonal antibody (Catalog # A00707-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF8 at approximately 65 kDa. The expected band size for RNF8 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00707-3-rnf8-primary-antibodies-fcm-testing-2.jpgAnti-RNF8 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-RNF8 antibody (A00707-3). Overlay histogram showing U937 cells stained with A00707-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF8 Antibody (A00707-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ring2-ring1b-rnf2-picoband-trade-antibody-a01209-3-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01209-3-rnf2-primary-antibodies-wb-testing-1.jpgAnti-RING2/RING1B/RNF2 Antibody Picoband® Western blot analysis of RING2/RING1B/RNF2 using anti-RING2/RING1B/RNF2 antibody (A01209-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RING2/RING1B/RNF2 antigen affinity purified polyclonal antibody (Catalog # A01209-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RING2/RING1B/RNF2 at approximately 38 kDa. The expected band size for RING2/RING1B/RNF2 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01209-3-rnf2-primary-antibodies-ihc-testing-2.jpgAnti-RING2/RING1B/RNF2 Antibody Picoband® IHC analysis of RING2/RING1B/RNF2 using anti-RING2/RING1B/RNF2 antibody (A01209-3). RING2/RING1B/RNF2 was detected in a paraffin-embedded section of human laryngeal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RING2/RING1B/RNF2 Antibody (A01209-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01209-3-rnf2-primary-antibodies-ihc-testing-3.jpgAnti-RING2/RING1B/RNF2 Antibody Picoband® IHC analysis of RING2/RING1B/RNF2 using anti-RING2/RING1B/RNF2 antibody (A01209-3). RING2/RING1B/RNF2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RING2/RING1B/RNF2 Antibody (A01209-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01209-3-rnf2-primary-antibodies-ihc-testing-4.jpgAnti-RING2/RING1B/RNF2 Antibody Picoband® IHC analysis of RING2/RING1B/RNF2 using anti-RING2/RING1B/RNF2 antibody (A01209-3). RING2/RING1B/RNF2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RING2/RING1B/RNF2 Antibody (A01209-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ror-gamma-rorc-picoband-trade-antibody-a00444-3-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00444-3-rorc-primary-antibodies-wb-testing-1.jpgAnti-ROR gamma/RORC Antibody Picoband® Western blot analysis of ROR gamma/RORC using anti-ROR gamma/RORC antibody (A00444-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR gamma/RORC antigen affinity purified polyclonal antibody (Catalog # A00444-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR gamma/RORC at approximately 58 kDa. The expected band size for ROR gamma/RORC is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00444-3-rorc-primary-antibodies-fcm-testing-2.jpgAnti-ROR gamma/RORC Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-ROR gamma/RORC antibody (A00444-3). Overlay histogram showing Hela cells stained with A00444-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROR gamma/RORC Antibody (A00444-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oat2-slc22a7-picoband-trade-antibody-a05273-2-boster.html2026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05273-2-slc22a7-primary-antibodies-wb-testing-1.jpgAnti-OAT2/SLC22A7 Antibody Picoband® Western blot analysis of OAT2/SLC22A7 using anti-OAT2/SLC22A7 antibody (A05273-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT2/SLC22A7 antigen affinity purified polyclonal antibody (Catalog # A05273-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT2/SLC22A7 at approximately 60 kDa. The expected band size for OAT2/SLC22A7 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05273-2-slc22a7-primary-antibodies-ihc-testing-2.jpgAnti-OAT2/SLC22A7 Antibody Picoband® IHC analysis of OAT2/SLC22A7 using anti-OAT2/SLC22A7 antibody (A05273-2). OAT2/SLC22A7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT2/SLC22A7 Antibody (A05273-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05273-2-slc22a7-primary-antibodies-ihc-testing-3.jpgAnti-OAT2/SLC22A7 Antibody Picoband® IHC analysis of OAT2/SLC22A7 using anti-OAT2/SLC22A7 antibody (A05273-2). OAT2/SLC22A7 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT2/SLC22A7 Antibody (A05273-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05273-2-slc22a7-primary-antibodies-ihc-testing-4.jpgAnti-OAT2/SLC22A7 Antibody Picoband® IHC analysis of OAT2/SLC22A7 using anti-OAT2/SLC22A7 antibody (A05273-2). OAT2/SLC22A7 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT2/SLC22A7 Antibody (A05273-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05273-2-slc22a7-primary-antibodies-fcm-testing-5.jpgAnti-OAT2/SLC22A7 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-OAT2/SLC22A7 antibody (A05273-2). Overlay histogram showing HepG2 cells stained with A05273-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OAT2/SLC22A7 Antibody (A05273-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slit2-picoband-trade-antibody-a01627-1-boster.html2026-03-17T05:13:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01627-1-slit2-primary-antibodies-wb-testing-1.jpgAnti-SLIT2 Antibody Picoband® Western blot analysis of SLIT2 using anti-SLIT2 antibody (A01627-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLIT2 antigen affinity purified polyclonal antibody (Catalog # A01627-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLIT2 at approximately 170 kDa. The expected band size for SLIT2 is at 170 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01627-1-slit2-primary-antibodies-fcm-testing-2.jpgAnti-SLIT2 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-SLIT2 antibody (A01627-1). Overlay histogram showing Daudi cells stained with A01627-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLIT2 Antibody (A01627-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-madh7-smad7-picoband-trade-antibody-a00784-2-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00784-2-smad7-primary-antibodies-wb-testing-1.jpgAnti-MADH7/SMAD7 Antibody Picoband® Western blot analysis of SMAD7 using anti-SMAD7 antibody (A00784-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD7 antigen affinity purified polyclonal antibody (Catalog # A00784-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD7 at approximately 50 kDa. The expected band size for SMAD7 is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-smpd1-picoband-trade-antibody-a00752-1-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00752-1-smpd1-primary-antibodies-wb-testing-1.jpgAnti-Smpd1 Antibody Picoband® Western blot analysis of Smpd1 using anti-Smpd1 antibody (A00752-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smpd1 antigen affinity purified polyclonal antibody (Catalog # A00752-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Smpd1 at approximately 70 kDa. The expected band size for Smpd1 is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-snail-snai1-picoband-trade-antibody-a00716-2-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00716-2-snai1-primary-antibodies-wb-testing-1.jpgAnti-SNAIL/SNAI1 Antibody Picoband® Western blot analysis of SNAIL/SNAI1 using anti-SNAIL/SNAI1 antibody (A00716-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Raji whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAIL/SNAI1 antigen affinity purified polyclonal antibody (Catalog # A00716-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAIL/SNAI1 at approximately 29 kDa. The expected band size for SNAIL/SNAI1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00716-2-snai1-primary-antibodies-fcm-testing-3.jpgAnti-SNAIL/SNAI1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SNAIL/SNAI1 antibody (A00716-2). https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00716-2-snai1-primary-antibodies-wb-testing-2.jpgAnti-SNAIL/SNAI1 Antibody Picoband® Western blot analysis of SNAIL/SNAI1 using anti-SNAIL/SNAI1 antibody (A00716-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat RH35 whole cell lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAIL/SNAI1 antigen affinity purified polyclonal antibody (Catalog # A00716-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAIL/SNAI1 at approximately 29 kDa. The expected band size for SNAIL/SNAI1 is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-snap23-picoband-trade-antibody-a02487-1-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-wb-testing-1.jpgAnti-SNAP23 Antibody Picoband® Western blot analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP23 antigen affinity purified polyclonal antibody (Catalog # A02487-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP23 at approximately 25 kDa. The expected band size for SNAP23 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-ihc-testing-6.jpgAnti-SNAP23 Antibody Picoband®IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). SNAP23 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-ihc-testing-2.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). SNAP23 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-ihc-testing-3.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). SNAP23 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-ihc-testing-4.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). SNAP23 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-ihc-testing-5.jpgAnti-SNAP23 Antibody Picoband® IHC analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). SNAP23 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAP23 Antibody (A02487-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-if-testing-6.jpgAnti-SNAP23 Antibody Picoband® IF analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). SNAP23 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNAP23 Antibody (A02487-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02487-1-snap23-primary-antibodies-fcm-testing-7.jpgAnti-SNAP23 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-SNAP23 antibody (A02487-1). Overlay histogram showing RT4 cells stained with A02487-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP23 Antibody (A02487-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-stat6-picoband-trade-antibody-a00523-4-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00523-4-stat6-primary-antibodies-wb-testing-1.jpgAnti-STAT6 Antibody Picoband® Western blot analysis of STAT6 using anti-STAT6 antibody (A00523-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 antigen affinity purified polyclonal antibody (Catalog # A00523-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT6 at approximately 100 kDa. The expected band size for STAT6 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00523-4-stat6-primary-antibodies-ihc-testing-2.jpgAnti-STAT6 Antibody Picoband® IHC analysis of STAT6 using anti-STAT6 antibody (A00523-4). STAT6 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT6 Antibody (A00523-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00523-4-stat6-primary-antibodies-ihc-testing-3.jpgAnti-STAT6 Antibody Picoband® IHC analysis of STAT6 using anti-STAT6 antibody (A00523-4). STAT6 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT6 Antibody (A00523-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00523-4-stat6-primary-antibodies-ihc-testing-4.jpgAnti-STAT6 Antibody Picoband® IHC analysis of STAT6 using anti-STAT6 antibody (A00523-4). STAT6 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT6 Antibody (A00523-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00523-4-stat6-primary-antibodies-ihc-testing-5.jpgAnti-STAT6 Antibody Picoband® IHC analysis of STAT6 using anti-STAT6 antibody (A00523-4). STAT6 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT6 Antibody (A00523-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00523-4-stat6-primary-antibodies-if-testing-6.jpgAnti-STAT6 Antibody Picoband® IF analysis of STAT6 using anti-STAT6 antibody (A00523-4). STAT6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4 μg/mL rabbit anti-STAT6 Antibody (A00523-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00523-4-stat6-primary-antibodies-fcm-testing-7.jpgAnti-STAT6 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-STAT6 antibody (A00523-4). Overlay histogram showing U937 cells stained with A00523-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT6 Antibody (A00523-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tlr4-picoband-trade-antibody-a00017-2-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00017-2-tlr4-primary-antibodies-wb-testing-1.jpgAnti-TLR4 Antibody Picoband® Western blot analysis of TLR4 using anti-TLR4 antibody (A00017-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR4 antigen affinity purified polyclonal antibody (Catalog # A00017-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR4 at approximately 100 kDa. The expected band size for TLR4 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00017-2-tlr4-primary-antibodies-fcm-testing-2.jpgAnti-TLR4 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-TLR4 antibody (A00017-2). Overlay histogram showing U937 cells stained with A00017-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR4 Antibody (A00017-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tomm20-picoband-trade-antibody-a04039-2-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-ihc-testing-3.jpgAnti-TOMM20 Antibody Picoband®IHC analysis of TOM20/TOMM20 using anti-TOM20/TOMM20 antibody (A04039-2). TOM20/TOMM20 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM20/TOMM20 Antibody (A04039-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-wb-testing-1.jpgAnti-TOMM20 Antibody Picoband®Western blot analysis of TOMM20 using anti-TOMM20 antibody (A04039-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM20 antigen affinity purified polyclonal antibody (A04039-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TOMM20 at approximately 16 kDa. The expected band size for TOMM20 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-ihc-testing-1.jpgAnti-TOMM20 Antibody Picoband®IHC analysis of TOMM20 using anti-TOMM20 antibody (A04039-2). TOMM20 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM20 Antibody (A04039-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-ihc-testing-2.jpgAnti-TOMM20 Antibody Picoband®IHC analysis of TOMM20 using anti-TOMM20 antibody (A04039-2). TOMM20 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM20 Antibody (A04039-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-if-testing-1.jpgAnti-TOMM20 Antibody Picoband®IF analysis of TOMM20 using anti-TOMM20 antibody (A04039-2). TOMM20 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOMM20 Antibody (A04039-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-if-testing-2.jpgAnti-TOMM20 Antibody Picoband®IF analysis of TOMM20 using anti-TOMM20 antibody (A04039-2). TOMM20 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TOMM20 Antibody (A04039-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-ip-testing-1.jpgAnti-TOMM20 Antibody Picoband®Immunoprecipitating TOMM20 in 293T whole cell lysate. Western blot analysis of TOMM20 using anti-TOMM20 antibody (A04039-2); Lane 1: 293T whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-TOMM20 antibody in 293T whole cell lysate; Lane 3: anti-TOMM20 antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TOMM20 antigen affinity purified polyclonal antibody (A04039-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for TOMM20 at approximately 16 kDa. The expected band size for TOMM20 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04039-2-tomm20-primary-antibodies-fcm-testing-1.jpgAnti-TOMM20 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-TOMM20 antibody (A04039-2). Overlay histogram showing HepG2 cells stained with A04039-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM20 Antibody (A04039-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trpv6-picoband-trade-antibody-a02139-2-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02139-2-trpv6-primary-antibodies-wb-testing-1.jpgAnti-TRPV6 Antibody Picoband® Western blot analysis of TRPV6 using anti-TRPV6 antibody (A02139-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HGC-27 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV6 antigen affinity purified polyclonal antibody (Catalog # A02139-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV6 at approximately 83 kDa. The expected band size for TRPV6 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02139-2-trpv6-primary-antibodies-fcm-testing-2.jpgAnti-TRPV6 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-TRPV6 antibody (A02139-2). Overlay histogram showing RT4 cells stained with A02139-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV6 Antibody (A02139-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bbs8-ttc8-picoband-trade-antibody-a07486-2-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02139-2-ttc8-primary-antibodies-wb-testing-1.jpgAnti-BBS8/TTC8 Antibody Picoband® Western blot analysis of BBS8/TTC8 using anti-BBS8/TTC8 antibody (A07486-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBS8/TTC8 antigen affinity purified polyclonal antibody (Catalog # A07486-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BBS8/TTC8 at approximately 23 kDa. The expected band size for BBS8/TTC8 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02139-2-ttc8-primary-antibodies-fcm-testing-2.jpgAnti-BBS8/TTC8 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-BBS8/TTC8 antibody (A07486-2). Overlay histogram showing RT4 cells stained with A07486-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BBS8/TTC8 Antibody (A07486-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-von-willebrand-factor-vwf-picoband-trade-antibody-a00270-1-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00270-1-vwf-primary-antibodies-ihc-testing-1.jpgAnti-Von Willebrand Factor/VWF Antibody IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00270-1-vwf-primary-antibodies-ihc-testing-2.jpgAnti-Von Willebrand Factor/VWF Antibody IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00270-1-vwf-primary-antibodies-ihc-testing-3.jpgAnti-Von Willebrand Factor/VWF Antibody IHC analysis of Von Willebrand Factor/VWF using anti-Von Willebrand Factor/VWF antibody (A00270-1). Von Willebrand Factor/VWF was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Von Willebrand Factor/VWF Antibody (A00270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-xaf1-picoband-trade-antibody-a03432-1-boster.html2026-03-17T05:13:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03432-1-xaf1-primary-antibodies-wb-testing-1.jpgAnti-XAF1 Antibody Picoband® Western blot analysis of XAF1 using anti-XAF1 antibody (A03432-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XAF1 antigen affinity purified polyclonal antibody (Catalog # A03432-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XAF1 at approximately 35 kDa. The expected band size for XAF1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03432-1-xaf1-primary-antibodies-ihc-testing-2.jpgAnti-XAF1 Antibody Picoband® IHC analysis of XAF1 using anti-XAF1 antibody (A03432-1). XAF1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XAF1 Antibody (A03432-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03432-1-xaf1-primary-antibodies-ihc-testing-3.jpgAnti-XAF1 Antibody Picoband® IHC analysis of XAF1 using anti-XAF1 antibody (A03432-1). XAF1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XAF1 Antibody (A03432-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03432-1-xaf1-primary-antibodies-ihc-testing-4.jpgAnti-XAF1 Antibody Picoband® IHC analysis of XAF1 using anti-XAF1 antibody (A03432-1). XAF1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XAF1 Antibody (A03432-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03432-1-xaf1-primary-antibodies-fcm-testing-5.jpgAnti-XAF1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-XAF1 antibody (A03432-1). Overlay histogram showing U87 cells stained with A03432-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XAF1 Antibody (A03432-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-zbtb48-picoband-trade-antibody-a13220-1-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13220-1-zbtb48-primary-antibodies-wb-testing-1.jpgAnti-ZBTB48 Antibody Picoband® Western blot analysis of ZBTB48 using anti-ZBTB48 antibody (A13220-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB48 antigen affinity purified polyclonal antibody (Catalog # A13220-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB48 at approximately 77 kDa. The expected band size for ZBTB48 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13220-1-zbtb48-primary-antibodies-fcm-testing-2.jpgAnti-ZBTB48 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ZBTB48 antibody (A13220-1). Overlay histogram showing SiHa cells stained with A13220-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZBTB48 Antibody (A13220-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aldh2-picoband-trade-antibody-monoclonal-m00546-2-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-2-aldh2-primary-antibodies-wb-testing-1_1_1.jpgAnti-ALDH2 Antibody Picoband®(monoclonal, 6H2) Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human SHG-44 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ALDH2 antigen affinity purified monoclonal antibody (Catalog # M00546-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-2-aldh2-primary-antibodies-wb-testing-2_1_1.jpgAnti-ALDH2 Antibody Picoband®(monoclonal, 6H2) Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ALDH2 antigen affinity purified monoclonal antibody (Catalog # M00546-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-2-aldh2-primary-antibodies-if-testing-3_1_1.jpgAnti-ALDH2 Antibody Picoband®(monoclonal, 6H2) IF analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). ALDH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL mouse anti-ALDH2 Antibody (M00546-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-2-aldh2-primary-antibodies-if-testing-4_1_1.jpgAnti-ALDH2 Antibody Picoband®(monoclonal, 6H2) IF analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). ALDH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL mouse anti-ALDH2 Antibody (M00546-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00546-2-aldh2-primary-antibodies-fc-testing-5_1_1.jpgAnti-ALDH2 Antibody Picoband®(monoclonal, 6H2) Flow Cytometry analysis of SiHa cells using anti-ALDH2 antibody (M00546-2). Overlay histogram showing SiHa cells stained with M00546-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ALDH2 Antibody (M00546-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-agps-picoband-trade-antibody-a03481-1-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-wb-testing-1.jpgAnti-AGPS Antibody Picoband® Western blot analysis of AGPS using anti-AGPS antibody (A03481-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGPS antigen affinity purified polyclonal antibody (Catalog # A03481-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGPS at approximately 73 kDa. The expected band size for AGPS is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-ihc-testing-2.jpgAnti-AGPS Antibody Picoband® IHC analysis of AGPS using anti-AGPS antibody (A03481-1). AGPS was detected in a paraffin-embedded section of human adenocarcinoma of lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPS Antibody (A03481-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-ihc-testing-3.jpgAnti-AGPS Antibody Picoband® IHC analysis of AGPS using anti-AGPS antibody (A03481-1). AGPS was detected in a paraffin-embedded section of human adenocarcinoma of lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPS Antibody (A03481-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-ihc-testing-4.jpgAnti-AGPS Antibody Picoband® IHC analysis of AGPS using anti-AGPS antibody (A03481-1). AGPS was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPS Antibody (A03481-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-ihc-testing-5.jpgAnti-AGPS Antibody Picoband® IHC analysis of AGPS using anti-AGPS antibody (A03481-1). AGPS was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPS Antibody (A03481-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-ihc-testing-6.jpgAnti-AGPS Antibody Picoband® IHC analysis of AGPS using anti-AGPS antibody (A03481-1). AGPS was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPS Antibody (A03481-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-ihc-testing-7.jpgAnti-AGPS Antibody Picoband® IHC analysis of AGPS using anti-AGPS antibody (A03481-1). AGPS was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGPS Antibody (A03481-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-if-testing-8.jpgAnti-AGPS Antibody Picoband® IF analysis of AGPS using anti-AGPS antibody (A03481-1). AGPS was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AGPS Antibody (A03481-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03481-1-agps-primary-antibodies-fcm-testing-9.jpgAnti-AGPS Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-AGPS antibody (A03481-1). Overlay histogram showing U937 cells stained with A03481-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGPS Antibody (A03481-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atp1b2-picoband-trade-antibody-a07027-2-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07027-2-atp1b2-primary-antibodies-wb-testing-1.jpgAnti-ATP1B2 Antibody Picoband® Western blot analysis of ATP1B2 using anti-ATP1B2 antibody (A07027-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1B2 antigen affinity purified polyclonal antibody (Catalog # A07027-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1B2 at approximately 44 kDa. The expected band size for ATP1B2 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07027-2-atp1b2-primary-antibodies-ihc-testing-2.jpgAnti-ATP1B2 Antibody Picoband® IHC analysis of ATP1B2 using anti-ATP1B2 antibody (A07027-2). ATP1B2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1B2 Antibody (A07027-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07027-2-atp1b2-primary-antibodies-fcm-testing-3.jpgAnti-ATP1B2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-ATP1B2 antibody (A07027-2). Overlay histogram showing U87 cells stained with A07027-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP1B2 Antibody (A07027-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atp5f1a-picoband-trade-antibody-a32267-2-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-wb-testing-1.jpgAnti-ATP5F1A Antibody Picoband® Western blot analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified polyclonal antibody (Catalog # A32267-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1A at approximately 54 kDa. The expected band size for ATP5F1A is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-ihc-testing-7.jpgAnti-ATP5F1A Antibody Picoband®IHC analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-wb-testing-2.jpgAnti-ATP5F1A Antibody Picoband® Western blot analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat H9C2(2-1) whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified polyclonal antibody (Catalog # A32267-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1A at approximately 54 kDa. The expected band size for ATP5F1A is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-ihc-testing-3.jpgAnti-ATP5F1A Antibody Picoband® IHC analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-ihc-testing-4.jpgAnti-ATP5F1A Antibody Picoband® IHC analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-ihc-testing-5.jpgAnti-ATP5F1A Antibody Picoband® IHC analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-ihc-testing-6.jpgAnti-ATP5F1A Antibody Picoband® IHC analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-if-testing-7.jpgAnti-ATP5F1A Antibody Picoband® IF analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-fcm-testing-8.jpgAnti-ATP5F1A Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-ATP5F1A antibody (A32267-2). Overlay histogram showing U937 cells stained with A32267-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5F1A Antibody (A32267-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-if-testing-9.jpgAnti-ATP5F1A Antibody Picoband® IF analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32267-2-atp5f1a-primary-antibodies-if-testing-10.jpgAnti-ATP5F1A Antibody Picoband® IF analysis of ATP5F1A using anti-ATP5F1A antibody (A32267-2). ATP5F1A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATP5F1A Antibody (A32267-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-atp5f1b-picoband-trade-antibody-a32270-3-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-wb-testing-1.jpgAnti-ATP5F1B Antibody Picoband® Western blot analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1B antigen affinity purified polyclonal antibody (Catalog # A32270-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1B at approximately 54 kDa. The expected band size for ATP5F1B is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-wb-testing-2.jpgAnti-ATP5F1B Antibody Picoband® Western blot analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mosue RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5F1B antigen affinity purified polyclonal antibody (Catalog # A32270-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5F1B at approximately 54 kDa. The expected band size for ATP5F1B is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-3.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-4.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-5.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-6.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-7.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-8.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-9.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-ihc-testing-10.jpgAnti-ATP5F1B Antibody Picoband® IHC analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-if-testing-11.jpgAnti-ATP5F1B Antibody Picoband® IF analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-fcm-testing-12.jpgAnti-ATP5F1B Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-ATP5F1B antibody (A32270-3). Overlay histogram showing 293T cells stained with A32270-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5F1B Antibody (A32270-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-if-testing-13.jpgAnti-ATP5F1B Antibody Picoband® IF analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32270-3-atp5f1b-primary-antibodies-if-testing-14.jpgAnti-ATP5F1B Antibody Picoband® IF analysis of ATP5F1B using anti-ATP5F1B antibody (A32270-3). ATP5F1B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATP5F1B Antibody (A32270-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-carbonic-anhydrase-9-ca9-picoband-trade-antibody-a01083-3-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01083-3-ca9-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase 9/CA9 Antibody Picoband® Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody (Catalog # A01083-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01083-3-ca9-primary-antibodies-ihc-testing-2.jpgAnti-Carbonic Anhydrase 9/CA9 Antibody Picoband® IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01083-3-ca9-primary-antibodies-ihc-testing-3.jpgAnti-Carbonic Anhydrase 9/CA9 Antibody Picoband® IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of human renal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01083-3-ca9-primary-antibodies-ihc-testing-4.jpgAnti-Carbonic Anhydrase 9/CA9 Antibody Picoband® IHC analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Carbonic Anhydrase 9/CA9 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01083-3-ca9-primary-antibodies-fcm-testing-5_2.jpgAnti-Carbonic Anhydrase 9/CA9 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Overlay histogram showing U87 cells stained with A01083-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01083-3-ca9-primary-antibodies-fcm-testing-6.jpgAnti-Carbonic Anhydrase 9/CA9 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Overlay histogram showing RAW264.7 cells stained with A01083-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 9/CA9 Antibody (A01083-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-chd1-picoband-trade-antibody-a01175-1-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01175-1-chd1-primary-antibodies-wb-testing-1.jpgAnti-CHD1 Antibody Picoband® Western blot analysis of CHD1 using anti-CHD1 antibody (A01175-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD1 antigen affinity purified polyclonal antibody (Catalog # A01175-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD1 at approximately 270 kDa. The expected band size for CHD1 is at 196 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01175-1-chd1-primary-antibodies-ihc-testing-2.jpgAnti-CHD1 Antibody Picoband® IHC analysis of CHD1 using anti-CHD1 antibody (A01175-1). CHD1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHD1 Antibody (A01175-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01175-1-chd1-primary-antibodies-ihc-testing-3.jpgAnti-CHD1 Antibody Picoband® IHC analysis of CHD1 using anti-CHD1 antibody (A01175-1). CHD1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHD1 Antibody (A01175-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01175-1-chd1-primary-antibodies-fcm-testing-4.jpgAnti-CHD1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-CHD1 antibody (A01175-1). Overlay histogram showing Hela cells stained with A01175-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHD1 Antibody (A01175-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cox5b-picoband-trade-antibody-a06090-2-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-wb-testing-1.jpgAnti-COX5B Antibody Picoband® Western blot analysis of COX5B using anti-COX5B antibody (A06090-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human MCF-7 whole cell lysates. Lane 7: human HCCT tissue lysates, Lane 8: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX5B antigen affinity purified polyclonal antibody (Catalog # A06090-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX5B at approximately 15 kDa. The expected band size for COX5B is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-wb-testing-2.jpgAnti-COX5B Antibody Picoband® Western blot analysis of COX5B using anti-COX5B antibody (A06090-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mosue heart tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mosue brain tissue lysates, Lane 7: mosue lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX5B antigen affinity purified polyclonal antibody (Catalog # A06090-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX5B at approximately 15 kDa. The expected band size for COX5B is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-3.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-4.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-5.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-6.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-7.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-8.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-9.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-10.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-11.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-ihc-testing-12.jpgAnti-COX5B Antibody Picoband® IHC analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0003) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-if-testing-13.jpgAnti-COX5B Antibody Picoband® IF analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-fcm-testing-14.jpgAnti-COX5B Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-COX5B antibody (A06090-2). Overlay histogram showing Hela cells stained with A06090-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX5B Antibody (A06090-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-fcm-testing-15.jpgAnti-COX5B Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-COX5B antibody (A06090-2). Overlay histogram showing 293T cells stained with A06090-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX5B Antibody (A06090-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-if-testing-16.jpgAnti-COX5B Antibody Picoband® IF analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06090-2-cox5b-primary-antibodies-if-testing-17.jpgAnti-COX5B Antibody Picoband® IF analysis of COX5B using anti-COX5B antibody (A06090-2). COX5B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-COX5B Antibody (A06090-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ctrl-picoband-trade-antibody-a08534-1-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08534-1-ctrl-primary-antibodies-wb-testing-1.jpgAnti-CTRL Antibody Picoband® Western blot analysis of CTRL using anti-CTRL antibody (A08534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PCP tissue lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse panceras tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTRL antigen affinity purified polyclonal antibody (Catalog # A08534-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTRL at approximately 28 kDa. The expected band size for CTRL is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08534-1-ctrl-primary-antibodies-fcm-testing-2.jpgAnti-CTRL Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CTRL antibody (A08534-1). Overlay histogram showing HepG2 cells stained with A08534-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CTRL Antibody (A08534-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ctsc-picoband-trade-antibody-a02136-2-boster.html2026-03-17T05:13:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02136-2-ctsc-primary-antibodies-wb-testing-1.jpgAnti-CTSC Antibody Picoband® Western blot analysis of CTSC using anti-CTSC antibody (A02136-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTSC antigen affinity purified polyclonal antibody (Catalog # A02136-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTSC at approximately 60 kDa. The expected band size for CTSC is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02136-2-ctsc-primary-antibodies-fcm-testing-2.jpgAnti-CTSC Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CTSC antibody (A02136-2). Overlay histogram showing U937 cells stained with A02136-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTSC Antibody (A02136-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-echs1-picoband-trade-antibody-a04508-1-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-wb-testing-1.jpgAnti-ECHS1 Antibody Picoband® Western blot analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody (Catalog # A04508-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECHS1 at approximately 29 kDa. The expected band size for ECHS1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-wb-testing-2.jpgAnti-ECHS1 Antibody Picoband® Western blot analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECHS1 antigen affinity purified polyclonal antibody (Catalog # A04508-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECHS1 at approximately 29 kDa. The expected band size for ECHS1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-ihc-testing-3.jpgAnti-ECHS1 Antibody Picoband® IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-ihc-testing-4.jpgAnti-ECHS1 Antibody Picoband® IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-ihc-testing-5.jpgAnti-ECHS1 Antibody Picoband® IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-ihc-testing-6.jpgAnti-ECHS1 Antibody Picoband® IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-ihc-testing-7.jpgAnti-ECHS1 Antibody Picoband® IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-ihc-testing-8.jpgAnti-ECHS1 Antibody Picoband® IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-ihc-testing-9.jpgAnti-ECHS1 Antibody Picoband® IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-if-testing-10.jpgAnti-ECHS1 Antibody Picoband® IF analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-if-testing-11.jpgAnti-ECHS1 Antibody Picoband® IF analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-if-testing-12.jpgAnti-ECHS1 Antibody Picoband® IF analysis of ECHS1 using anti-ECHS1 antibody (A04508-1). ECHS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ECHS1 Antibody (A04508-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04508-1-echs1-primary-antibodies-fcm-testing-13.jpgAnti-ECHS1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-ECHS1 antibody (A04508-1). Overlay histogram showing 293T cells stained with A04508-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECHS1 Antibody (A04508-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eif3a-picoband-trade-antibody-a02339-3-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02339-3-eif3a-primary-antibodies-wb-testing-1.jpgAnti-EIF3A Antibody Picoband® Western blot analysis of EIF3A using anti-EIF3A antibody (A02339-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: rat L6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3A antigen affinity purified polyclonal antibody (Catalog # A02339-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3A at approximately 180 kDa. The expected band size for EIF3A is at 167 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02339-3-eif3a-primary-antibodies-ihc-testing-2.jpgAnti-EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (A02339-3). EIF3A was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (A02339-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02339-3-eif3a-primary-antibodies-ihc-testing-3.jpgAnti-EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (A02339-3). EIF3A was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (A02339-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02339-3-eif3a-primary-antibodies-ihc-testing-4.jpgAnti-EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (A02339-3). EIF3A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (A02339-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02339-3-eif3a-primary-antibodies-ihc-testing-5.jpgAnti-EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (A02339-3). EIF3A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (A02339-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02339-3-eif3a-primary-antibodies-fcm-testing-6.jpgAnti-EIF3A Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-EIF3A antibody (A02339-3). Overlay histogram showing U87 cells stained with A02339-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF3A Antibody (A02339-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02339-3-eif3a-primary-antibodies-if-testing-7.jpgAnti-EIF3A Antibody Picoband® IF analysis of EIF3A using anti-EIF3A antibody (A02339-3). EIF3A was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EIF3A Antibody (A02339-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cd135-flt3-picoband-trade-antibody-a00188-5-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00188-5-flt3-primary-antibodies-wb-testing-1.jpgAnti-CD135/FLT3 Antibody Picoband® Western blot analysis of CD135/FLT3 using anti-CD135/FLT3 antibody (A00188-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD135/FLT3 antigen affinity purified polyclonal antibody (Catalog # A00188-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD135/FLT3 at approximately 160 kDa. The expected band size for CD135/FLT3 is at 113 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00188-5-flt3-primary-antibodies-ihc-testing-2.jpgAnti-CD135/FLT3 Antibody Picoband® IHC analysis of CD135/FLT3 using anti-CD135/FLT3 antibody (A00188-5). CD135/FLT3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD135/FLT3 Antibody (A00188-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00188-5-flt3-primary-antibodies-ihc-testing-3.jpgAnti-CD135/FLT3 Antibody Picoband®IHC analysis of FLT3 using anti-FLT3 antibody (A00188-5). FLT3 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLT3 Antibody (A00188-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-tim-3-havcr2-picoband-trade-antibody-a00657-4-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00657-4-havcr2-primary-antibodies-wb-testing-1.jpgAnti-TIM 3/HAVCR2 Antibody Picoband® Western blot analysis of TIM 3/HAVCR2 using anti-TIM 3/HAVCR2 antibody (A00657-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3/HAVCR2 antigen affinity purified polyclonal antibody (Catalog # A00657-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3/HAVCR2 at approximately 55 kDa. The expected band size for TIM 3/HAVCR2 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00657-4-havcr2-primary-antibodies-fcm-testing-2.jpgAnti-TIM 3/HAVCR2 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TIM 3/HAVCR2 antibody (A00657-4). Overlay histogram showing HEL cells stained with A00657-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIM 3/HAVCR2 Antibody (A00657-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hax1-picoband-trade-antibody-a01495-2-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01495-2-hax1-primary-antibodies-wb-testing-1.jpgAnti-HAX1 Antibody Picoband® Western blot analysis of HAX1 using anti-HAX1 antibody (A01495-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAX1 antigen affinity purified polyclonal antibody (Catalog # A01495-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAX1 at approximately 36 kDa. The expected band size for HAX1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01495-2-hax1-primary-antibodies-ihc-testing-2.jpgAnti-HAX1 Antibody Picoband® IHC analysis of HAX1 using anti-HAX1 antibody (A01495-2). HAX1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAX1 Antibody (A01495-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01495-2-hax1-primary-antibodies-ihc-testing-3.jpgAnti-HAX1 Antibody Picoband® IHC analysis of HAX1 using anti-HAX1 antibody (A01495-2). HAX1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAX1 Antibody (A01495-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01495-2-hax1-primary-antibodies-ihc-testing-4.jpgAnti-HAX1 Antibody Picoband® IHC analysis of HAX1 using anti-HAX1 antibody (A01495-2). HAX1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAX1 Antibody (A01495-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01495-2-hax1-primary-antibodies-fcm-testing-5.jpgAnti-HAX1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-HAX1 antibody (A01495-2). Overlay histogram showing HEL cells stained with A01495-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HAX1 Antibody (A01495-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eg5-kif11-picoband-trade-antibody-a01754-2-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01754-2-kif11-primary-antibodies-wb-testing-1.jpgAnti-Eg5/KIF11 Antibody Picoband® Western blot analysis of Eg5/KIF11 using anti-Eg5/KIF11 antibody (A01754-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eg5/KIF11 antigen affinity purified polyclonal antibody (Catalog # A01754-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eg5/KIF11 at approximately 130 kDa. The expected band size for Eg5/KIF11 is at 119 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01754-2-kif11-primary-antibodies-ihc-testing-2.jpgAnti-Eg5/KIF11 Antibody Picoband® IHC analysis of Eg5/KIF11 using anti-Eg5/KIF11 antibody (A01754-2). Eg5/KIF11 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Eg5/KIF11 Antibody (A01754-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01754-2-kif11-primary-antibodies-ihc-testing-3.jpgAnti-Eg5/KIF11 Antibody Picoband® IHC analysis of Eg5/KIF11 using anti-Eg5/KIF11 antibody (A01754-2). Eg5/KIF11 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Eg5/KIF11 Antibody (A01754-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01754-2-kif11-primary-antibodies-ihc-testing-4.jpgAnti-Eg5/KIF11 Antibody Picoband® IHC analysis of Eg5/KIF11 using anti-Eg5/KIF11 antibody (A01754-2). Eg5/KIF11 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Eg5/KIF11 Antibody (A01754-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01754-2-kif11-primary-antibodies-if-testing-5.jpgAnti-Eg5/KIF11 Antibody Picoband® IF analysis of Eg5/KIF11 using anti-Eg5/KIF11 antibody (A01754-2). Eg5/KIF11 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Eg5/KIF11 Antibody (A01754-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01754-2-kif11-primary-antibodies-fcm-testing-6.jpgAnti-Eg5/KIF11 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-Eg5/KIF11 antibody (A01754-2). Overlay histogram showing U937 cells stained with A01754-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Eg5/KIF11 Antibody (A01754-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-klf13-picoband-trade-antibody-a07076-2-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07076-2-klf13-primary-antibodies-wb-testing-1.jpgAnti-KLF13 Antibody Picoband® Western blot analysis of KLF13 using anti-KLF13 antibody (A07076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF13 antigen affinity purified polyclonal antibody (Catalog # A07076-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF13 at approximately 38 kDa. The expected band size for KLF13 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07076-2-klf13-primary-antibodies-ihc-testing-3.jpgAnti-KLF13 Antibody Picoband®IHC analysis of KLF13 using anti-KLF13 antibody (A07076-2). KLF13 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLF13 Antibody (A07076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07076-2-klf13-primary-antibodies-ihc-testing-2.jpgAnti-KLF13 Antibody Picoband® IHC analysis of KLF13 using anti-KLF13 antibody (A07076-2). KLF13 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLF13 Antibody (A07076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07076-2-klf13-primary-antibodies-fcm-testing-3.jpgAnti-KLF13 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-KLF13 antibody (A07076-2). Overlay histogram showing Hela cells stained with A07076-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KLF13 Antibody (A07076-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07076-2-klf13-primary-antibodies-fcm-testing-4.jpgAnti-KLF13 Antibody Picoband® Flow Cytometry analysis of Neuro-2a cells using anti-KLF13 antibody (A07076-2). Overlay histogram showing Neuro-2a cells stained with A07076-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KLF13 Antibody (A07076-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07076-2-klf13-primary-antibodies-fcm-testing-5.jpgAnti-KLF13 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-KLF13 antibody (A07076-2). Overlay histogram showing C6 cells stained with A07076-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KLF13 Antibody (A07076-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mrps18b-picoband-trade-antibody-a12773-1-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12773-1-mrps18b-primary-antibodies-wb-testing-1.jpgAnti-MRPS18B Antibody Picoband® Western blot analysis of MRPS18B using anti-MRPS18B antibody (A12773-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human SH-SY5Y whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRPS18B antigen affinity purified polyclonal antibody (Catalog # A12773-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRPS18B at approximately 26 kDa. The expected band size for MRPS18B is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12773-1-mrps18b-primary-antibodies-ihc-testing-2.jpgAnti-MRPS18B Antibody Picoband® IHC analysis of MRPS18B using anti-MRPS18B antibody (A12773-1). MRPS18B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRPS18B Antibody (A12773-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12773-1-mrps18b-primary-antibodies-ihc-testing-3.jpgAnti-MRPS18B Antibody Picoband® IHC analysis of MRPS18B using anti-MRPS18B antibody (A12773-1). MRPS18B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRPS18B Antibody (A12773-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12773-1-mrps18b-primary-antibodies-if-testing-4.jpgAnti-MRPS18B Antibody Picoband® IF analysis of MRPS18B using anti-MRPS18B antibody (A12773-1). MRPS18B was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MRPS18B Antibody (A12773-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12773-1-mrps18b-primary-antibodies-fcm-testing-5.jpgAnti-MRPS18B Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-MRPS18B antibody (A12773-1). Overlay histogram showing Hela cells stained with A12773-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MRPS18B Antibody (A12773-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mrps22-picoband-trade-antibody-a10300-2-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-wb-testing-1.jpgAnti-MRPS22 Antibody Picoband® Western blot analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human Hacat whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRPS22 antigen affinity purified polyclonal antibody (Catalog # A10300-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRPS22 at approximately 38 kDa. The expected band size for MRPS22 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-ihc-testing-2.jpgAnti-MRPS22 Antibody Picoband® IHC analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). MRPS22 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRPS22 Antibody (A10300-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-ihc-testing-3.jpgAnti-MRPS22 Antibody Picoband® IHC analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). MRPS22 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRPS22 Antibody (A10300-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-ihc-testing-4.jpgAnti-MRPS22 Antibody Picoband® IHC analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). MRPS22 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRPS22 Antibody (A10300-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-ihc-testing-5.jpgAnti-MRPS22 Antibody Picoband® IHC analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). MRPS22 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRPS22 Antibody (A10300-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-ihc-testing-6.jpgAnti-MRPS22 Antibody Picoband® IHC analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). MRPS22 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MRPS22 Antibody (A10300-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-if-testing-7.jpgAnti-MRPS22 Antibody Picoband® IF analysis of MRPS22 using anti-MRPS22 antibody (A10300-2). MRPS22 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MRPS22 Antibody (A10300-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10300-2-mrps22-primary-antibodies-fcm-testing-8_1.jpgAnti-MRPS22 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-MRPS22 antibody (A10300-2). Overlay histogram showing Hela cells stained with A10300-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MRPS22 Antibody (A10300-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufs2-picoband-trade-antibody-a05618-3-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-wb-testing-1.jpgAnti-NDUFS2 Antibody Picoband® Western blot analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS2 antigen affinity purified polyclonal antibody (Catalog # A05618-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS2 at approximately 45 kDa. The expected band size for NDUFS2 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-13062_2025_593_fig4_html.jpgAnti-NDUFS2 Antibody Picoband®Machine learning and MR analysis of hub MitoDEGs nominate three causal MitoDEGs for COPD. a The mean decrease in accuracy (MeanDecreaseAccuracy) (left) and mean decrease in Gini (MeanDecreaseGini) (right) were used to rank the relative importance of the 18 selected feature MitoDEGs for COPD which were selected by RF analysis following the filter criteria as both MeanDecreaseAccuracy and MeanDecreaseGini > 5. b Assessing the performance of the RF model using the receiver operating characteristic curve (ROC), the area under the curve (AUC) values were 0.916 in the training set, 0.902 in the test set, 0.881 in the internal validation dataset ( ), and 0.828 in the external validation dataset ( ), reflecting the overall stability and reliability of the RF model in ( a ). c LASSO regression showed log(λ) = 5.726 when the error of the model is minimized (top), and 13 MitoDEGs from 18 feature MitoDEGs identified by RF analysis in ( a ) were selected (bottom). d Forest plot showing the MR estimates of 6 hub MitoDEGs ( MRPL27 , MRPL2 , NDUFS2 , CAT , SCP2 , HSD17B4 ) and COPD risk using the inverse variance weighting (IVW) method together with the results of sensitivity analyses. Pval represents p-value for MR analysis, P.pleio represents p-value for horizontal pleiotropy analysis, P.heter.ivw represents p-value for heterozygosity with IVW method, P.heter.mr represents p-value for heterozygosity with MR-Egger method. e Scatter plots showing the causal effect of MRPL27 , MRPL2 , NDUFS2 , CAT , and HSD17B4 on COPD. f Leave-one-out plot demonstrating the causal effect of MRPL27 , MRPL2 , NDUFS2 , CAT , and HSD17B4 on COPD when leaving one SNP out. g Venn diagram showing the intersection of 18 feature genes identified by RF analysis (green), 13 signature genes selected by LASSO regression analysis (blue), and 5 causal genes nominated by MR analysis (red). Only NDUFS2 , MRPL2 , and CAT appeared simultaneously in the results of three analyses Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11715544/'>39789601</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-13062_2025_593_fig5_html.jpgAnti-NDUFS2 Antibody Picoband®Reanalysis of causal MitoDEGs reveals their expression distribution and risk prediction value. a UMAP plot of the 14 identified cell types is used to visualize the result of clustering of cells from the scRNA-seq dataset . One dot represents a cell. One color represents a cell type. b Feature expression of NDUFS2 , MRPL2 , and CAT on UMAP plots of all the cells. The color ranging from light red to dark red represents the expression level of the gene from low to high. c Violin plots showing the expression of NDUFS2 , MRPL2 , and CAT in human AMs isolated from COPD patients and control individuals of bulk RNA-seq dataset . d The nomogram model for predicting risk for COPD with expression levels of NDUFS2 , MRPL2 , and CAT in pulmonary macrophages. The expression level of each gene is located on each variable axis, and a line is drawn on top to determine the number of points received for each variable value. The total points projected at the bottom scale indicate the risk for COPD. e The calibration curve of nomogram in ( d ). B = 1000 repetitions boot. Mean absolute error = 0.04 Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11715544/'>39789601</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-13062_2025_593_fig6_html.jpgAnti-NDUFS2 Antibody Picoband®Functional analysis suggests the modulatory role of NDUFS2 in macrophage function and communication. a Split violin plot showing the expression distributions of NDUFS2 in pulmonary macrophages among control and COPD patients with or without smoking histories. b Bar plot showing GSVA scores of the top altered KEGG pathways in macrophages NDUFS2 low (blue) and macrophages NDUFS2 high (green). c GSEA analysis highlighting activated inflammatory response and suppressed phagocytosis as well as endocytosis in macrophages NDUFS2 low as compared with macrophages NDUFS2 high . Circle plots ( d ) showing the altered outgoing (left) and incoming (right) intercellular communications of macrophages NDUFS2 low as compared with macrophages NDUFS2 high (the thicker the line, the greater the change of interaction), which was overall quantified and visualized as a heatmap ( e ), coupled with the significant signaling pathways being ranked based on their differences of overall information flow within the outgoing (left) and incoming (right) communication networks between macrophages NDUFS2 low and macrophages NDUFS2 high ( f ). Circle plots ( g ) showing the altered outgoing (left) and incoming (right) intercellular communications of macrophages NDUFS2 low from COPD patients as compared with macrophages NDUFS2 high from control individuals, which was overall quantified and visualized as a heatmap ( h ), coupled with the significant signaling pathways being ranked based on their differences of overall information flow within the outgoing (left) and incoming (right) communication networks between macrophages NDUFS2 low from COPD patients and macrophages NDUFS2 high from control individuals ( i ) Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11715544/'>39789601</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-13062_2025_593_fig7_html.jpgAnti-NDUFS2 Antibody Picoband®Expression of NDUFS2 was decreased in AMs from COPD mice. a Eight-week-old C57BL/6 mice were exposed to CS to construct COPD mouse model, while the littermates exposed to room air for the same period served as control ( n = 6). The protein levels of NDUFS2 (red) and F4/80 (green) in these mice lungs were measured by immunofluorescence staining (Scale bars = 50 μm). The overall expression levels of NDUFS2 and F4/80 in mouse lung sections were quantified as the red and green fluorescence intensity, respectively. The expression level of NDUFS2 in pulmonary macrophages (marked by F4/80) was quantified as the proportion of NDUFS2 + F4/80 + cells in total F4/80. + cells. ** P < 0.01 vs. Ctrl (Student’s t -test). Scale bars = 50 μm. b , c Eight-week-old C57BL/6 mice were exposed to CS to construct COPD mouse model, while the littermates exposed to room air for the same period served as control ( n = 6). The mRNA ( b ) and protein ( c ) levels of Ndufs2 were measured by qPCR and immunofluorescence staining in the primary AMs isolated from each mouse. The overall expression levels of NDUFS2 and HSP60 in AMs were quantified as the red and green fluorescence intensity, respectively. The representative images of AMs at low magnification were displayed (Scale bars = 20 μm) in the white solid box at bottom left corner of every enlarged image at higher magnification (Scale bars = 10 μm). The protein levels of NDUFS2 (red) in AMs (marked by HSP60, green) was quantified as red fluorescence intensity. ** P < 0.01 vs. Ctrl (Student’s t -test) Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11715544/'>39789601</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-13062_2025_593_fig8_html.jpgAnti-NDUFS2 Antibody Picoband®Downregulation of NDUFS2 leads to pro-inflammatory phenotype and disrupted phagocytosis in macrophages. The protein levels of NDUFS2 (red) and HSP60 (green) were measured by immunofluorescence staining in TP cells ( a ) and RAW264.7 cells ( b ). Scale bars = 20 μm for representative images at low magnification in the white solid box. Scale bars = 10 μm for enlarged image at higher magnification. The protein levels of NDUFS2 (red) in AMs (marked by HSP60, green) was quantified as red fluorescence intensity. TP cells ( c ) and RAW264.7 cells ( d ) were transfected with or without 3 different pieces of siRNAs against NDUFS2/Ndufs2 for 48 h, when the mRNA and protein levels of NDUFS2/Ndufs2 were measured by qPCR (left) and western blotting (right). si NDUFS2 -2 and si Ndufs2 -3 were selected for further experiments in TP cells and RAW264.7 cells, respectively. * P < 0.01, ** P < 0.01 vs. siNC (Student’s t -test). e, f TP cells ( e ) and RAW264.7 cells ( f ) were transfected with si NDUFS2 -2 and si Ndufs2 -3 for 48 h respectively y, when the mRNA levels of IL1B , IL6 , TLR4 , TGFB1 , NLRP3 , IFNG , IL23 , STAT3 , TNF , CD80 , CXCL8 , CXCL10 , CXCL11 , CXCL12 , CCL2 , CD209 , MACRO , SIRPA were measured by qPCR. * P < 0.01, ** P < 0.01 vs. siNC (Student’s t -test). g , h TP cells ( g ) and RAW264.7 cells ( h ) were transfected with si NDUFS2 -2 and si Ndufs2 -3 for 48 h respectively, when the phagocytosis was assessed by Cell Meter™ Fluorimetric Phagocytosis Assay Kit. The images were taken under fluorescence microscopy (Scale bars = 20 μm). The red fluorescence intensity of phagocytosed beads reflects the digestion extent of the ingested particles in macrophages, while the average number of engulfed beads within every cell was calculated to indicate the engulfing ability of macrophages. ** P < 0.01, *** P < 0.001 or vs. siNC. (Student’s t -test) Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11715544/'>39789601</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-ihc-testing-2.jpgAnti-NDUFS2 Antibody Picoband® IHC analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-ihc-testing-3.jpgAnti-NDUFS2 Antibody Picoband® IHC analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-ihc-testing-4.jpgAnti-NDUFS2 Antibody Picoband® IHC analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-ihc-testing-5.jpgAnti-NDUFS2 Antibody Picoband® IHC analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-ihc-testing-6.jpgAnti-NDUFS2 Antibody Picoband® IHC analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-ihc-testing-7.jpgAnti-NDUFS2 Antibody Picoband® IHC analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-if-testing-8.jpgAnti-NDUFS2 Antibody Picoband® IF analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-fcm-testing-9.jpgAnti-NDUFS2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-NDUFS2 antibody (A05618-3). Overlay histogram showing Hela cells stained with A05618-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS2 Antibody (A05618-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-if-testing-10.jpgAnti-NDUFS2 Antibody Picoband® IF analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05618-3-ndufs2-primary-antibodies-if-testing-11.jpgAnti-NDUFS2 Antibody Picoband® IF analysis of NDUFS2 using anti-NDUFS2 antibody (A05618-3). NDUFS2 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFS2 Antibody (A05618-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ndufs6-picoband-trade-antibody-a09082-2-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09082-2-ndufs6-primary-antibodies-wb-testing-1.jpgAnti-NDUFS6 Antibody Picoband® Western blot analysis of NDUFS6 using anti-NDUFS6 antibody (A09082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS6 antigen affinity purified polyclonal antibody (Catalog # A09082-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS6 at approximately 14 kDa. The expected band size for NDUFS6 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09082-2-ndufs6-primary-antibodies-wb-testing-2.jpgAnti-NDUFS6 Antibody Picoband® Western blot analysis of NDUFS6 using anti-NDUFS6 antibody (A09082-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS6 antigen affinity purified polyclonal antibody (Catalog # A09082-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS6 at approximately 14 kDa. The expected band size for NDUFS6 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09082-2-ndufs6-primary-antibodies-ihc-testing-3.jpgAnti-NDUFS6 Antibody Picoband® IHC analysis of NDUFS6 using anti-NDUFS6 antibody (A09082-2). NDUFS6 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS6 Antibody (A09082-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09082-2-ndufs6-primary-antibodies-ihc-testing-4.jpgAnti-NDUFS6 Antibody Picoband® IHC analysis of NDUFS6 using anti-NDUFS6 antibody (A09082-2). NDUFS6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS6 Antibody (A09082-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09082-2-ndufs6-primary-antibodies-ihc-testing-5.jpgAnti-NDUFS6 Antibody Picoband® IHC analysis of NDUFS6 using anti-NDUFS6 antibody (A09082-2). NDUFS6 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS6 Antibody (A09082-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09082-2-ndufs6-primary-antibodies-fcm-testing-6_1.jpgAnti-NDUFS6 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-NDUFS2 antibody (A05618-3). Overlay histogram showing Hela cells stained with A05618-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS2 Antibody (A05618-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufs8-picoband-trade-antibody-a08275-2-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08275-2-ndufs8-primary-antibodies-wb-testing-1.jpgAnti-NDUFS8 Antibody Picoband® Western blot analysis of NDUFS8 using anti-NDUFS8 antibody (A08275-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS8 antigen affinity purified polyclonal antibody (Catalog # A08275-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS8 at approximately 23 kDa. The expected band size for NDUFS8 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08275-2-ndufs8-primary-antibodies-wb-testing-2.jpgAnti-NDUFS8 Antibody Picoband® Western blot analysis of NDUFS8 using anti-NDUFS8 antibody (A08275-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS8 antigen affinity purified polyclonal antibody (Catalog # A08275-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS8 at approximately 23 kDa. The expected band size for NDUFS8 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08275-2-ndufs8-primary-antibodies-ihc-testing-3.jpgAnti-NDUFS8 Antibody Picoband® IHC analysis of NDUFS8 using anti-NDUFS8 antibody (A08275-2). NDUFS8 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS8 Antibody (A08275-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08275-2-ndufs8-primary-antibodies-ihc-testing-4.jpgAnti-NDUFS8 Antibody Picoband® IHC analysis of NDUFS8 using anti-NDUFS8 antibody (A08275-2). NDUFS8 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS8 Antibody (A08275-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08275-2-ndufs8-primary-antibodies-fcm-testing-5.jpgAnti-NDUFS8 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-NDUFS8 antibody (A08275-2). Overlay histogram showing Hela cells stained with A08275-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS8 Antibody (A08275-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08275-2-ndufs8-primary-antibodies-fcm-testing-6_1.jpgAnti-NDUFS8 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-NDUFS8 antibody (A08275-2). Overlay histogram showing 293T cells stained with A08275-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS8 Antibody (A08275-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08275-2-ndufs8-primary-antibodies-if-testing-7.jpgAnti-NDUFS8 Antibody Picoband® IF analysis of NDUFS8 using anti-NDUFS8 antibody (A08275-2). NDUFS8 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFS8 Antibody (A08275-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-nedd4-picoband-trade-antibody-a00984-4-boster.html2026-03-17T05:13:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-4-nedd4-primary-antibodies-wb-testing-1.jpgAnti-Nedd4 Antibody Picoband® Western blot analysis of Nedd4 using anti-Nedd4 antibody (A00984-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat L6 whole cell lysates, Lane 2: mouse skeletal muscle tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse B16-F10 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nedd4 antigen affinity purified polyclonal antibody (Catalog # A00984-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nedd4 at approximately 115 kDa. The expected band size for Nedd4 is at 149 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-4-nedd4-primary-antibodies-ihc-testing-2.jpgAnti-Nedd4 Antibody Picoband® IHC analysis of Nedd4 using anti-Nedd4 antibody (A00984-4). Nedd4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nedd4 Antibody (A00984-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-4-nedd4-primary-antibodies-ihc-testing-3.jpgAnti-Nedd4 Antibody Picoband® IHC analysis of Nedd4 using anti-Nedd4 antibody (A00984-4). Nedd4 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nedd4 Antibody (A00984-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-4-nedd4-primary-antibodies-ihc-testing-4.jpgAnti-Nedd4 Antibody Picoband® IHC analysis of Nedd4 using anti-Nedd4 antibody (A00984-4). Nedd4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nedd4 Antibody (A00984-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-4-nedd4-primary-antibodies-ihc-testing-5.jpgAnti-Nedd4 Antibody Picoband® IHC analysis of Nedd4 using anti-Nedd4 antibody (A00984-4). Nedd4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nedd4 Antibody (A00984-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-4-nedd4-primary-antibodies-fcm-testing-6.jpgAnti-Nedd4 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Nedd4 antibody (A00984-4). Overlay histogram showing HEPA1-6 cells stained with A00984-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nedd4 Antibody (A00984-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00984-4-nedd4-primary-antibodies-if-testing-7.jpgAnti-Nedd4 Antibody Picoband® IF analysis of Nedd4 using anti-Nedd4 antibody (A00984-4). Nedd4 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Nedd4 Antibody (A00984-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-nop58-picoband-trade-antibody-a05070-1-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05070-1-nop58-primary-antibodies-wb-testing-1.jpgAnti-NOP58 Antibody Picoband® Western blot analysis of NOP58 using anti-NOP58 antibody (A05070-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP58 antigen affinity purified polyclonal antibody (Catalog # A05070-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOP58 at approximately 70 kDa. The expected band size for NOP58 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05070-1-nop58-primary-antibodies-if-testing-2.jpgAnti-NOP58 Antibody Picoband® IF analysis of NOP58 using anti-NOP58 antibody (A05070-1). NOP58 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NOP58 Antibody (A05070-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05070-1-nop58-primary-antibodies-fcm-testing-3.jpgAnti-NOP58 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-NOP58 antibody (A05070-1). Overlay histogram showing U251 cells stained with A05070-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOP58 Antibody (A05070-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05070-1-nop58-primary-antibodies-fcm-testing-4.jpgAnti-NOP58 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-NOP58 antibody (A05070-1). Overlay histogram showing U937 cells stained with A05070-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOP58 Antibody (A05070-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-inos-nos2-picoband-trade-antibody-a00368-4-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00368-4-nos2-primary-antibodies-wb-testing-1_1.jpgAnti-iNOS/Nos2 Antibody Picoband® Western blot analysis of iNOS/Nos2 using anti-iNOS/Nos2 antibody (A00368-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7(-LPS) whole cell lysates, Lane 2: mouse RAW264.7(+LPS) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-iNOS/Nos2 antigen affinity purified polyclonal antibody (Catalog # A00368-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for iNOS/Nos2 at approximately 130 kDa. The expected band size for iNOS/Nos2 is at 130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00368-4-fpsyt-09-00434-g006.jpgAnti-iNOS/Nos2 Antibody Picoband®Effect of fluoxetine treatment on BDNF and inflammation levels. (A) Western blot results of NF-κB, IL-10, iNOS, BDNF, and GADPH. Quantification analysis of (B) NF-κB, (C) iNOS, (D) IL-10, and (E) BDNF. Values are expressed as mean ± SEM ( n > 5). N+C+P-FLX vs. N+C+P-con, * P < 0.05, ** P < 0.01, *** P < 0.001; N+C+P-con vs. N+P, * P < 0.05; A+C+P-FLX vs. A+C+P-con, * P < 0.05, *** P < 0.001; A+C+P-con vs. A+P, * P < 0.05; N+C+P+FLX vs. A+C+P-FLX, ∧∧ P < 0.01; N+C+P-con vs. A+C+P-con, && P < 0.01; N+P vs. A+P, # P < 0.05. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/psychiatry/articles/10.3389/fpsyt.2018.00434/full'>30349488</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00368-4-nos2-primary-antibodies-fcm-testing-2.jpgAnti-iNOS/Nos2 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-iNOS/Nos2 antibody (A00368-4). Overlay histogram showing HEPA1-6 cells stained with A00368-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-iNOS/Nos2 Antibody (A00368-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-app-1-pabpc4-picoband-trade-antibody-a07960-2-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-wb-testing-1.jpgAnti-APP-1/PABPC4 Antibody Picoband® Western blot analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human Daudi whole cell lysates, Lane 8: rat testis tissue lysates, Lane 9: rat heart tissue lysates, Lane 10: rat brain tissue lysates, Lane 11: rat L6 whole cell lysates, Lane 12: mouse heart tissue lysates, Lane 13: mouse brain tissue lysates, Lane 14: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP-1/PABPC4 antigen affinity purified polyclonal antibody (Catalog # A07960-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP-1/PABPC4 at approximately 75 kDa. The expected band size for APP-1/PABPC4 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-ihc-testing-2.jpgAnti-APP-1/PABPC4 Antibody Picoband® IHC analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2). APP-1/PABPC4 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-ihc-testing-3.jpgAnti-APP-1/PABPC4 Antibody Picoband® IHC analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2). APP-1/PABPC4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-if-testing-4.jpgAnti-APP-1/PABPC4 Antibody Picoband® IF analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2). APP-1/PABPC4 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-fcm-testing-5.jpgAnti-APP-1/PABPC4 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-APP-1/PABPC4 antibody (A07960-2). Overlay histogram showing HEL cells stained with A07960-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APP-1/PABPC4 Antibody (A07960-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-fcm-testing-6.jpgAnti-APP-1/PABPC4 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-APP-1/PABPC4 antibody (A07960-2). Overlay histogram showing RAW264.7 cells stained with A07960-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APP-1/PABPC4 Antibody (A07960-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-if-testing-7.jpgAnti-APP-1/PABPC4 Antibody Picoband® IF analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2). APP-1/PABPC4 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07960-2-pabpc4-primary-antibodies-if-testing-8.jpgAnti-APP-1/PABPC4 Antibody Picoband® IF analysis of APP-1/PABPC4 using anti-APP-1/PABPC4 antibody (A07960-2). APP-1/PABPC4 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-APP-1/PABPC4 Antibody (A07960-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-pdip-pdia2-picoband-trade-antibody-a03275-1-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03275-1-pdia2-primary-antibodies-wb-testing-1.jpgAnti-PDIP/PDIA2 Antibody Picoband® Western blot analysis of PDIP/PDIA2 using anti-PDIP/PDIA2 antibody (A03275-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIP/PDIA2 antigen affinity purified polyclonal antibody (Catalog # A03275-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDIP/PDIA2 at approximately 65 kDa. The expected band size for PDIP/PDIA2 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03275-1-pdia2-primary-antibodies-fcm-testing-2.jpgAnti-PDIP/PDIA2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PDIP/PDIA2 antibody (A03275-1). Overlay histogram showing 293T cells stained with A03275-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDIP/PDIA2 Antibody (A03275-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-psmb4-picoband-trade-antibody-a05105-1-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-wb-testing-1.jpgAnti-PSMB4 Antibody Picoband® Western blot analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB4 antigen affinity purified polyclonal antibody (Catalog # A05105-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB4 at approximately 25,29 kDa. The expected band size for PSMB4 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-ihc-testing-2.jpgAnti-PSMB4 Antibody Picoband® IHC analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-ihc-testing-3.jpgAnti-PSMB4 Antibody Picoband® IHC analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-ihc-testing-4.jpgAnti-PSMB4 Antibody Picoband® IHC analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-ihc-testing-5.jpgAnti-PSMB4 Antibody Picoband® IHC analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-ihc-testing-6.jpgAnti-PSMB4 Antibody Picoband® IHC analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-ihc-testing-7.jpgAnti-PSMB4 Antibody Picoband® IHC analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-fcm-testing-8.jpgAnti-PSMB4 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PSMB4 antibody (A05105-1). Overlay histogram showing HEL cells stained with A05105-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB4 Antibody (A05105-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-if-testing-9.jpgAnti-PSMB4 Antibody Picoband® IF analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05105-1-psmb4-primary-antibodies-if-testing-10.jpgAnti-PSMB4 Antibody Picoband® IF analysis of PSMB4 using anti-PSMB4 antibody (A05105-1). PSMB4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMB4 Antibody (A05105-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rpl36-picoband-trade-antibody-a08922-3-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-wb-testing-1.jpgAnti-RPL36 Antibody Picoband® Western blot analysis of RPL36 using anti-RPL36 antibody (A08922-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human U-937 whole cell lysates, Lane 8: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL36 antigen affinity purified polyclonal antibody (Catalog # A08922-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL36 at approximately 16 kDa. The expected band size for RPL36 is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-wb-testing-2.jpgAnti-RPL36 Antibody Picoband® Western blot analysis of RPL36 using anti-RPL36 antibody (A08922-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL36 antigen affinity purified polyclonal antibody (Catalog # A08922-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL36 at approximately 16 kDa. The expected band size for RPL36 is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-ihc-testing-3.jpgAnti-RPL36 Antibody Picoband® IHC analysis of RPL36 using anti-RPL36 antibody (A08922-3). RPL36 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL36 Antibody (A08922-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-ihc-testing-4.jpgAnti-RPL36 Antibody Picoband® IHC analysis of RPL36 using anti-RPL36 antibody (A08922-3). RPL36 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL36 Antibody (A08922-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-ihc-testing-5.jpgAnti-RPL36 Antibody Picoband® IHC analysis of RPL36 using anti-RPL36 antibody (A08922-3). RPL36 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL36 Antibody (A08922-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-ihc-testing-6.jpgAnti-RPL36 Antibody Picoband® IHC analysis of RPL36 using anti-RPL36 antibody (A08922-3). RPL36 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL36 Antibody (A08922-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-ihc-testing-7.jpgAnti-RPL36 Antibody Picoband® IHC analysis of RPL36 using anti-RPL36 antibody (A08922-3). RPL36 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL36 Antibody (A08922-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-fcm-testing-8.jpgAnti-RPL36 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RPL36 antibody (A08922-3). Overlay histogram showing HEL cells stained with A08922-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL36 Antibody (A08922-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-if-testing-9.jpgAnti-RPL36 Antibody Picoband® IF analysis of RPL36 using anti-RPL36 antibody (A08922-3). RPL36 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RPL36 Antibody (A08922-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08922-3-rpl36-primary-antibodies-if-testing-10.jpgAnti-RPL36 Antibody Picoband® IF analysis of RPL36 using anti-RPL36 antibody (A08922-3). RPL36 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RPL36 Antibody (A08922-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rrs1-picoband-trade-antibody-a04418-4-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-wb-testing-1.jpgAnti-RRS1 Antibody Picoband® Western blot analysis of RRS1 using anti-RRS1 antibody (A04418-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human U251 whole cell lysates, Lane 7: human Daudi whole cell lysates, Lane 8: human MOLT-4 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRS1 antigen affinity purified polyclonal antibody (Catalog # A04418-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRS1 at approximately 41 kDa. The expected band size for RRS1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-ihc-testing-2.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-4). RRS1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-ihc-testing-3.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-4). RRS1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-ihc-testing-4.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-4). RRS1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-ihc-testing-5.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-4). RRS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-ihc-testing-6.jpgAnti-RRS1 Antibody Picoband® IHC analysis of RRS1 using anti-RRS1 antibody (A04418-4). RRS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRS1 Antibody (A04418-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-if-testing-7.jpgAnti-RRS1 Antibody Picoband® IF analysis of RRS1 using anti-RRS1 antibody (A04418-4). RRS1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RRS1 Antibody (A04418-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-if-testing-8.jpgAnti-RRS1 Antibody Picoband® IF analysis of RRS1 using anti-RRS1 antibody (A04418-4). RRS1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RRS1 Antibody (A04418-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04418-4-rrs1-primary-antibodies-fcm-testing-9.jpgAnti-RRS1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RRS1 antibody (A04418-4). Overlay histogram showing HEL cells stained with A04418-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRS1 Antibody (A04418-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tip49a-ruvbl1-picoband-trade-antibody-a02049-2-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-wb-testing-1.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® Western blot analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse testis tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIP49A/RUVBL1 antigen affinity purified polyclonal antibody (Catalog # A02049-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIP49A/RUVBL1 at approximately 54 kDa. The expected band size for TIP49A/RUVBL1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-ihc-testing-2.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-ihc-testing-3.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-ihc-testing-4.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-ihc-testing-5.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-ihc-testing-6.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-ihc-testing-7.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-if-testing-8.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IF analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-if-testing-9.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® IF analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). TIP49A/RUVBL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-ip-testing-1.jpgAnti-TIP49A/RUVBL1 Antibody Picoband®Immunoprecipitating (IP) TIP49A/RUVBL1 in K562 whole cell lysate. Western blot analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2); Lane 1: K562 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-TIP49A/RUVBL1 antibody in K562 whole cell lysate; Lane 3: anti-TIP49A/RUVBL1 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TIP49A/RUVBL1 antigen affinity purified polyclonal antibody (A02049-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light Chian). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for TIP49A/RUVBL1 at approximately 54 kDa. The expected band size for TIP49A/RUVBL1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02049-2-ruvbl1-primary-antibodies-fcm-testing-10_1.jpgAnti-TIP49A/RUVBL1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-TIP49A/RUVBL1 antibody (A02049-2). Overlay histogram showing Hela cells stained with A02049-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-larp-septin1-picoband-trade-antibody-a30537-1-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30537-1-septin1-primary-antibodies-wb-testing-1.jpgAnti-LARP/SEPTIN1 Antibody Picoband® Western blot analysis of LARP/SEPTIN1 using anti-LARP/SEPTIN1 antibody (A30537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse EL-4 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARP/SEPTIN1 antigen affinity purified polyclonal antibody (Catalog # A30537-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARP/SEPTIN1 at approximately 40 kDa. The expected band size for LARP/SEPTIN1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30537-1-septin1-primary-antibodies-fcm-testing-2.jpgAnti-LARP/SEPTIN1 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-LARP/SEPTIN1 antibody (A30537-1). Overlay histogram showing RAW264.7 cells stained with A30537-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LARP/SEPTIN1 Antibody (A30537-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-septin-2-septin2-picoband-trade-antibody-a34007-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-wb-testing-1.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® Western blot analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human HEL whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 2/SEPTIN2 antigen affinity purified polyclonal antibody (Catalog # A34007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 2/SEPTIN2 at approximately 41 kDa. The expected band size for Septin 2/SEPTIN2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-wb-testing-2.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® Western blot analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 2/SEPTIN2 antigen affinity purified polyclonal antibody (Catalog # A34007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 2/SEPTIN2 at approximately 41 kDa. The expected band size for Septin 2/SEPTIN2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-ihc-testing-3.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IHC analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-ihc-testing-4.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IHC analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-ihc-testing-5.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IHC analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-ihc-testing-6.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IHC analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-if-testing-7.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IF analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-fcm-testing-8_1.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Septin 2/SEPTIN2 antibody (A34007). Overlay histogram showing U251 cells stained with A34007 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Septin 2/SEPTIN2 Antibody (A34007, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-if-testing-9.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IF analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-if-testing-10.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IF analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a34007-septin2-primary-antibodies-if-testing-11.jpgAnti-Septin 2/SEPTIN2 Antibody Picoband® IF analysis of Septin 2/SEPTIN2 using anti-Septin 2/SEPTIN2 antibody (A34007). Septin 2/SEPTIN2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Septin 2/SEPTIN2 Antibody (A34007) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-septin-3-septin3-picoband-trade-antibody-a30742-1-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30742-1-septin3-primary-antibodies-wb-testing-1.jpgAnti-Septin 3/SEPTIN3 Antibody Picoband® Western blot analysis of Septin 3/SEPTIN3 using anti-Septin 3/SEPTIN3 antibody (A30742-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 3/SEPTIN3 antigen affinity purified polyclonal antibody (Catalog # A30742-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 3/SEPTIN3 at approximately 41 kDa. The expected band size for Septin 3/SEPTIN3 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30742-1-septin3-primary-antibodies-ihc-testing-4.jpgAnti-Septin 3/SEPTIN3 Antibody Picoband®IHC analysis of Septin 3/SEPTIN3 using anti-Septin 3/SEPTIN3 antibody (A30742-1). Septin 3/SEPTIN3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 3/SEPTIN3 Antibody (A30742-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30742-1-septin3-primary-antibodies-ihc-testing-2.jpgAnti-Septin 3/SEPTIN3 Antibody Picoband® IHC analysis of Septin 3/SEPTIN3 using anti-Septin 3/SEPTIN3 antibody (A30742-1). Septin 3/SEPTIN3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 3/SEPTIN3 Antibody (A30742-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30742-1-septin3-primary-antibodies-ihc-testing-3.jpgAnti-Septin 3/SEPTIN3 Antibody Picoband® IHC analysis of Septin 3/SEPTIN3 using anti-Septin 3/SEPTIN3 antibody (A30742-1). Septin 3/SEPTIN3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 3/SEPTIN3 Antibody (A30742-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30742-1-septin3-primary-antibodies-if-testing-4.jpgAnti-Septin 3/SEPTIN3 Antibody Picoband® IF analysis of Septin 3/SEPTIN3 using anti-Septin 3/SEPTIN3 antibody (A30742-1). Septin 3/SEPTIN3 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Septin 3/SEPTIN3 Antibody (A30742-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30742-1-septin3-primary-antibodies-fcm-testing-5.jpgAnti-Septin 3/SEPTIN3 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Septin 3/SEPTIN3 antibody (A30742-1). Overlay histogram showing U251 cells stained with A30742-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Septin 3/SEPTIN3 Antibody (A30742-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sept4-septin4-picoband-trade-antibody-a09411-2-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09411-2-septin4-primary-antibodies-wb-testing-1.jpgAnti-SEPT4/SEPTIN4 Antibody Picoband® Western blot analysis of SEPT4/SEPTIN4 using anti-SEPT4/SEPTIN4 antibody (A09411-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT4/SEPTIN4 antigen affinity purified polyclonal antibody (Catalog # A09411-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT4/SEPTIN4 at approximately 50 kDa. The expected band size for SEPT4/SEPTIN4 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09411-2-septin4-primary-antibodies-fcm-testing-2.jpgAnti-SEPT4/SEPTIN4 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SEPT4/SEPTIN4 antibody (A09411-2). Overlay histogram showing HEL cells stained with A09411-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT4/SEPTIN4 Antibody (A09411-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09411-2-septin4-primary-antibodies-fcm-testing-3_1.jpgAnti-SEPT4/SEPTIN4 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-SEPT4/SEPTIN4 antibody (A09411-2). Overlay histogram showing Hela cells stained with A09411-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT4/SEPTIN4 Antibody (A09411-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sept7-septin7-picoband-trade-antibody-a30759-1-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30759-1-septin7-primary-antibodies-wb-testing-1.jpgAnti-SEPT7/SEPTIN7 Antibody Picoband® Western blot analysis of SEPT7/SEPTIN7 using anti-SEPT7/SEPTIN7 antibody (A30759-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human A375 whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat stomach tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse Neuro-2a whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT7/SEPTIN7 antigen affinity purified polyclonal antibody (Catalog # A30759-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT7/SEPTIN7 at approximately 51 kDa. The expected band size for SEPT7/SEPTIN7 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30759-1-septin7-primary-antibodies-ihc-testing-2.jpgAnti-SEPT7/SEPTIN7 Antibody Picoband® IHC analysis of SEPT7/SEPTIN7 using anti-SEPT7/SEPTIN7 antibody (A30759-1). SEPT7/SEPTIN7 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEPT7/SEPTIN7 Antibody (A30759-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30759-1-septin7-primary-antibodies-ihc-testing-3.jpgAnti-SEPT7/SEPTIN7 Antibody Picoband® IHC analysis of SEPT7/SEPTIN7 using anti-SEPT7/SEPTIN7 antibody (A30759-1). SEPT7/SEPTIN7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEPT7/SEPTIN7 Antibody (A30759-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30759-1-septin7-primary-antibodies-ihc-testing-4.jpgAnti-SEPT7/SEPTIN7 Antibody Picoband® IHC analysis of SEPT7/SEPTIN7 using anti-SEPT7/SEPTIN7 antibody (A30759-1). SEPT7/SEPTIN7 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEPT7/SEPTIN7 Antibody (A30759-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30759-1-septin7-primary-antibodies-if-testing-5.jpgAnti-SEPT7/SEPTIN7 Antibody Picoband® IF analysis of SEPT7/SEPTIN7 using anti-SEPT7/SEPTIN7 antibody (A30759-1). SEPT7/SEPTIN7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEPT7/SEPTIN7 Antibody (A30759-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30759-1-septin7-primary-antibodies-fcm-testing-6.jpgAnti-SEPT7/SEPTIN7 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-SEPT7/SEPTIN7 antibody (A30759-1). Overlay histogram showing Hela cells stained with A30759-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT7/SEPTIN7 Antibody (A30759-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-septin-8-septin8-picoband-trade-antibody-a30760-1-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30760-1-septin8-primary-antibodies-wb-testing-1.jpgAnti-Septin 8/Septin8 Antibody Picoband® Western blot analysis of Septin 8/Septin8 using anti-Septin 8/Septin8 antibody (A30760-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse brain tissue lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 8/Septin8 antigen affinity purified polyclonal antibody (Catalog # A30760-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 8/Septin8 at approximately 56 kDa. The expected band size for Septin 8/Septin8 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a30760-1-septin8-primary-antibodies-fcm-testing-2_1.jpgAnti-Septin 8/Septin8 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-Septin 8/Septin8 antibody (A30760-1). Overlay histogram showing RAW264.7 cells stained with A30760-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Septin 8/Septin8 Antibody (A30760-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-msf-septin9-picoband-trade-antibody-a05279-3-boster.html2026-03-17T05:13:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-wb-testing-1.jpgAnti-MSF/SEPTIN9 Antibody Picoband® Western blot analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human SH-SY5Y whole cell lsates, Lane 8: huamn PC-3 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSF/SEPTIN9 antigen affinity purified polyclonal antibody (Catalog # A05279-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSF/SEPTIN9 at approximately 70 kDa. The expected band size for MSF/SEPTIN9 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-ihc-testing-2.jpgAnti-MSF/SEPTIN9 Antibody Picoband® IHC analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). MSF/SEPTIN9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSF/SEPTIN9 Antibody (A05279-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-ihc-testing-3.jpgAnti-MSF/SEPTIN9 Antibody Picoband® IHC analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). MSF/SEPTIN9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSF/SEPTIN9 Antibody (A05279-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-ihc-testing-4.jpgAnti-MSF/SEPTIN9 Antibody Picoband® IHC analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). MSF/SEPTIN9 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSF/SEPTIN9 Antibody (A05279-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-ihc-testing-5.jpgAnti-MSF/SEPTIN9 Antibody Picoband® IHC analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). MSF/SEPTIN9 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSF/SEPTIN9 Antibody (A05279-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-if-testing-6.jpgAnti-MSF/SEPTIN9 Antibody Picoband® IF analysis of SEPTIN9 using anti-SEPTIN9 antibody (A05279-3). SEPTIN9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEPTIN9 Antibody (A05279-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-if-testing-7.jpgAnti-MSF/SEPTIN9 Antibody Picoband® IF analysis of SEPTIN9 using anti-SEPTIN9 antibody (A05279-3). SEPTIN9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEPTIN9 Antibody (A05279-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-fcm-testing-8.jpgAnti-MSF/SEPTIN9 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-MSF/SEPTIN9 antibody (A05279-3). Overlay histogram showing Hela cells stained with A05279-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSF/SEPTIN9 Antibody (A05279-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05279-3-septin9-primary-antibodies-fcm-testing-9.jpgAnti-MSF/SEPTIN9 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-MSF/SEPTIN9 antibody (A05279-3). Overlay histogram showing U251 cells stained with A05279-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSF/SEPTIN9 Antibody (A05279-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-oct1-slc22a1-picoband-trade-antibody-a01945-1-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01945-1-slc22a1-primary-antibodies-wb-testing-1.jpgAnti-OCT1/Slc22a1 Antibody Picoband® Western blot analysis of OCT1/Slc22a1 using anti-OCT1/Slc22a1 antibody (A01945-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCT1/Slc22a1 antigen affinity purified polyclonal antibody (Catalog # A01945-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCT1/Slc22a1 at approximately 75 kDa. The expected band size for OCT1/Slc22a1 is at 62 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slu7-picoband-trade-antibody-a07557-2-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07557-2-slu7-primary-antibodies-wb-testing-1.jpgAnti-SLU7 Antibody Picoband® Western blot analysis of SLU7 using anti-SLU7 antibody (A07557-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLU7 antigen affinity purified polyclonal antibody (Catalog # A07557-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLU7 at approximately 68 kDa. The expected band size for SLU7 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07557-2-slu7-primary-antibodies-ihc-testing-2.jpgAnti-SLU7 Antibody Picoband® IHC analysis of SLU7 using anti-SLU7 antibody (A07557-2). SLU7 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLU7 Antibody (A07557-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07557-2-slu7-primary-antibodies-ihc-testing-3.jpgAnti-SLU7 Antibody Picoband® IHC analysis of SLU7 using anti-SLU7 antibody (A07557-2). SLU7 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLU7 Antibody (A07557-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07557-2-slu7-primary-antibodies-ihc-testing-4.jpgAnti-SLU7 Antibody Picoband® IHC analysis of SLU7 using anti-SLU7 antibody (A07557-2). SLU7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLU7 Antibody (A07557-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07557-2-slu7-primary-antibodies-if-testing-5.jpgAnti-SLU7 Antibody Picoband® IF analysis of SLU7 using anti-SLU7 antibody (A07557-2). SLU7 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLU7 Antibody (A07557-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07557-2-slu7-primary-antibodies-if-testing-6.jpgAnti-SLU7 Antibody Picoband® IF analysis of SLU7 using anti-SLU7 antibody (A07557-2). SLU7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLU7 Antibody (A07557-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07557-2-slu7-primary-antibodies-fcm-testing-7.jpgAnti-SLU7 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-SLU7 antibody (A07557-2). Overlay histogram showing Hela cells stained with A07557-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLU7 Antibody (A07557-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-socs1-picoband-trade-antibody-a00448-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00448-socs1-primary-antibodies-wb-testing-1.jpgAnti-SOCS1 Antibody Picoband® Western blot analysis of SOCS1 using anti-SOCS1 antibody (A00448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS1 antigen affinity purified polyclonal antibody (Catalog # A00448) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS1 at approximately 27 kDa. The expected band size for SOCS1 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00448-socs1-primary-antibodies-wb-testing-2.jpgAnti-SOCS1 Antibody Picoband® Western blot analysis of SOCS1 using anti-SOCS1 antibody (A00448). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse ANA-1 whole cell lysates, Lane 6: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS1 antigen affinity purified polyclonal antibody (Catalog # A00448) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS1 at approximately 27 kDa. The expected band size for SOCS1 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00448-socs1-primary-antibodies-ihc-testing-3.jpgAnti-SOCS1 Antibody Picoband® IHC analysis of SOCS1 using anti-SOCS1 antibody (A00448). SOCS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS1 Antibody (A00448) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00448-socs1-primary-antibodies-ihc-testing-4.jpgAnti-SOCS1 Antibody Picoband® IHC analysis of SOCS1 using anti-SOCS1 antibody (A00448). SOCS1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS1 Antibody (A00448) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00448-socs1-primary-antibodies-ihc-testing-5.jpgAnti-SOCS1 Antibody Picoband® IHC analysis of SOCS1 using anti-SOCS1 antibody (A00448). SOCS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS1 Antibody (A00448) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00448-socs1-primary-antibodies-ihc-testing-6.jpgAnti-SOCS1 Antibody Picoband® IHC analysis of SOCS1 using anti-SOCS1 antibody (A00448). SOCS1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS1 Antibody (A00448) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00448-socs1-primary-antibodies-ihc-testing-7.jpgAnti-SOCS1 Antibody Picoband® IHC analysis of SOCS1 using anti-SOCS1 antibody (A00448). SOCS1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOCS1 Antibody (A00448) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-trbp-tarbp2-picoband-trade-antibody-a01680-2-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01680-2-tarbp2-primary-antibodies-wb-testing-1.jpgAnti-TRBP/TARBP2 Antibody Picoband® Western blot analysis of TRBP/TARBP2 using anti-TRBP/TARBP2 antibody (A01680-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRBP/TARBP2 antigen affinity purified polyclonal antibody (Catalog # A01680-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRBP/TARBP2 at approximately 44 kDa. The expected band size for TRBP/TARBP2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01680-2-tarbp2-primary-antibodies-ihc-testing-2.jpgAnti-TRBP/TARBP2 Antibody Picoband® IHC analysis of TRBP/TARBP2 using anti-TRBP/TARBP2 antibody (A01680-2). TRBP/TARBP2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRBP/TARBP2 Antibody (A01680-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01680-2-tarbp2-primary-antibodies-ihc-testing-3.jpgAnti-TRBP/TARBP2 Antibody Picoband® IHC analysis of TRBP/TARBP2 using anti-TRBP/TARBP2 antibody (A01680-2). TRBP/TARBP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRBP/TARBP2 Antibody (A01680-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01680-2-tarbp2-primary-antibodies-ihc-testing-4.jpgAnti-TRBP/TARBP2 Antibody Picoband® IHC analysis of TRBP/TARBP2 using anti-TRBP/TARBP2 antibody (A01680-2). TRBP/TARBP2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRBP/TARBP2 Antibody (A01680-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01680-2-tarbp2-primary-antibodies-ihc-testing-5.jpgAnti-TRBP/TARBP2 Antibody Picoband® IHC analysis of TRBP/TARBP2 using anti-TRBP/TARBP2 antibody (A01680-2). TRBP/TARBP2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRBP/TARBP2 Antibody (A01680-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01680-2-tarbp2-primary-antibodies-if-testing-6.jpgAnti-TRBP/TARBP2 Antibody Picoband® IF analysis of TRBP/TARBP2 using anti-TRBP/TARBP2 antibody (A01680-2). TRBP/TARBP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRBP/TARBP2 Antibody (A01680-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01680-2-tarbp2-primary-antibodies-fcm-testing-7_1_1.jpgAnti-TRBP/TARBP2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-TRBP/TARBP2 antibody (A01680-2). Overlay histogram showing Jurkat cells stained with A01680-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRBP/TARBP2 Antibody (A01680-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tfeb-picoband-trade-antibody-a00662-4-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-wb-testing-1.jpgAnti-TFEB Antibody Picoband® Western blot analysis of TFEB using anti-TFEB antibody (A00662-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody (Catalog # A00662-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFEB at approximately 60 kDa. The expected band size for TFEB is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-wb-testing-2.jpgAnti-TFEB Antibody Picoband® Western blot analysis of TFEB using anti-TFEB antibody (A00662-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFEB antigen affinity purified polyclonal antibody (Catalog # A00662-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFEB at approximately 60 kDa. The expected band size for TFEB is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-ihc-testing-3.jpgAnti-TFEB Antibody Picoband® IHC analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-ihc-testing-4.jpgAnti-TFEB Antibody Picoband® IHC analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-ihc-testing-5.jpgAnti-TFEB Antibody Picoband® IHC analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-ihc-testing-6.jpgAnti-TFEB Antibody Picoband® IHC analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-ihc-testing-7.jpgAnti-TFEB Antibody Picoband® IHC analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-ihc-testing-8.jpgAnti-TFEB Antibody Picoband® IHC analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-ihc-testing-9.jpgAnti-TFEB Antibody Picoband® IHC analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-if-testing-10.jpgAnti-TFEB Antibody Picoband® IF analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-fcm-testing-11_1.jpgAnti-TFEB Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-TFEB antibody (A00662-4). Overlay histogram showing U937 cells stained with A00662-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFEB Antibody (A00662-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-if-testing-12.jpgAnti-TFEB Antibody Picoband® IF analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00662-4-tfeb-primary-antibodies-if-testing-13.jpgAnti-TFEB Antibody Picoband® IF analysis of TFEB using anti-TFEB antibody (A00662-4). TFEB was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TFEB Antibody (A00662-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-usp22-picoband-trade-antibody-a03887-2-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03887-2-usp22-primary-antibodies-wb-testing-1.jpgAnti-USP22 Antibody Picoband® Western blot analysis of USP22 using anti-USP22 antibody (A03887-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human MOLT-4 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP22 antigen affinity purified polyclonal antibody (Catalog # A03887-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP22 at approximately 68 kDa. The expected band size for USP22 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03887-2-usp22-primary-antibodies-wb-testing-2.jpgAnti-USP22 Antibody Picoband® Western blot analysis of USP22 using anti-USP22 antibody (A03887-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP22 antigen affinity purified polyclonal antibody (Catalog # A03887-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP22 at approximately 68 kDa. The expected band size for USP22 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-vpac2-vipr2-picoband-trade-antibody-a03768-1-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03768-1-vipr2-primary-antibodies-wb-testing-1.jpgAnti-VPAC2/VIPR2 Antibody Picoband® Western blot analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPAC2/VIPR2 antigen affinity purified polyclonal antibody (Catalog # A03768-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPAC2/VIPR2 at approximately 49 kDa. The expected band size for VPAC2/VIPR2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03768-1-vipr2-primary-antibodies-ihc-testing-2_1.jpgAnti-VPAC2/VIPR2 Antibody Picoband® IHC analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1). VPAC2/VIPR2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPAC2/VIPR2 Antibody (A03768-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03768-1-vipr2-primary-antibodies-ihc-testing-3.jpgAnti-VPAC2/VIPR2 Antibody Picoband® IHC analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1). VPAC2/VIPR2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPAC2/VIPR2 Antibody (A03768-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03768-1-vipr2-primary-antibodies-ihc-testing-4.jpgAnti-VPAC2/VIPR2 Antibody Picoband® IHC analysis of VPAC2/VIPR2 using anti-VPAC2/VIPR2 antibody (A03768-1). VPAC2/VIPR2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPAC2/VIPR2 Antibody (A03768-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-14-3-3-gamma-ywhag-picoband-trade-antibody-a04148-2-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04148-2-ywhag-primary-antibodies-wb-testing-1.jpgAnti-14-3-3 gamma/YWHAG Antibody Picoband® Western blot analysis of 14-3-3 gamma/YWHAG using anti-14-3-3 gamma/YWHAG antibody (A04148-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 gamma/YWHAG antigen affinity purified polyclonal antibody (Catalog # A04148-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 gamma/YWHAG at approximately 28 kDa. The expected band size for 14-3-3 gamma/YWHAG is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04148-2-ywhag-primary-antibodies-ihc-testing-2.jpgAnti-14-3-3 gamma/YWHAG Antibody Picoband® IHC analysis of 14-3-3 gamma/YWHAG using anti-14-3-3 gamma/YWHAG antibody (A04148-2). 14-3-3 gamma/YWHAG was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-14-3-3 gamma/YWHAG Antibody (A04148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04148-2-ywhag-primary-antibodies-fcm-testing-3.jpgAnti-14-3-3 gamma/YWHAG Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-14-3-3 gamma/YWHAG antibody (A04148-2). Overlay histogram showing U87 cells stained with A04148-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 gamma/YWHAG Antibody (A04148-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-zw10-picoband-trade-antibody-a04266-1-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04266-1-zw10-primary-antibodies-wb-testing-1.jpgAnti-ZW10 Antibody Picoband® Western blot analysis of ZW10 using anti-ZW10 antibody (A04266-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZW10 antigen affinity purified polyclonal antibody (Catalog # A04266-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZW10 at approximately 95 kDa. The expected band size for ZW10 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04266-1-zw10-primary-antibodies-fcm-testing-2.jpgAnti-ZW10 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-ZW10 antibody (A04266-1). Overlay histogram showing 293T cells stained with A04266-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZW10 Antibody (A04266-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bag2-picoband-trade-antibody-m04933-2-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04933-2-bag2-primary-antibodies-wb-testing-1.jpgAnti-BAG2 Antibody Picoband® (monoclonal, 8F11G2) Western blot analysis of BAG2 using anti-BAG2 antibody (M04933-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-BAG2 antigen affinity purified monoclonal antibody (Catalog # M04933-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04933-2-bag2-primary-antibodies-fcm-testing-2.jpgAnti-BAG2 Antibody Picoband® (monoclonal, 8F11G2) Flow Cytometry analysis of K562 cells using anti-BAG2 antibody (M04933-2). Overlay histogram showing K562 cells stained with M04933-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-BAG2 Antibody (M04933-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-areb6-zeb1-picoband-trade-antibody-m00548-2-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-wb-testing-1.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) Western blot analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AREB6/ZEB1 antigen affinity purified monoclonal antibody (Catalog # M00548-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for AREB6/ZEB1 at approximately 200 kDa. The expected band size for AREB6/ZEB1 is at 124 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-2.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-3.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-4.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-5.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-6.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-7.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-8.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-9.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-ihc-testing-10.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-if-testing-11.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IF analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-2-zeb1-primary-antibodies-if-testing-12.jpgAnti-AREB6/ZEB1 Antibody Picoband® (monoclonal, 8B12D7) IF analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody (M00548-2). AREB6/ZEB1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-AREB6/ZEB1 Antibody (M00548-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nkcc1-slc12a2-picoband-trade-antibody-m03603-boster.html2026-03-25T05:24:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03603-slc12a2-primary-antibodies-wb-testing-1.jpgAnti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) Western blot analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NKCC1/SLC12A2 antigen affinity purified monoclonal antibody (Catalog # M03603) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NKCC1/SLC12A2 at approximately 200 kDa. The expected band size for NKCC1/SLC12A2 is at 131 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03603-slc12a2-primary-antibodies-ihc-testing-2.jpgAnti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603). NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03603-slc12a2-primary-antibodies-ihc-testing-3.jpgAnti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603). NKCC1/SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03603-slc12a2-primary-antibodies-ihc-testing-4.jpgAnti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603). NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03603-slc12a2-primary-antibodies-if-testing-5.jpgAnti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603). NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03603-slc12a2-primary-antibodies-if-testing-6.jpgAnti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603). NKCC1/SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03603-slc12a2-primary-antibodies-if-testing-7.jpgAnti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603). NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cpsf6-picoband-trade-antibody-m04551-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-wb-testing-1.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) Western blot analysis of CPSF6 using anti-CPSF6 antibody (M04551). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CPSF6 antigen affinity purified monoclonal antibody (Catalog # M04551) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CPSF6 at approximately 68 kDa. The expected band size for CPSF6 is at 59 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-ihc-testing-2.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IHC analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-ihc-testing-3.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IHC analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-ihc-testing-4.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IHC analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-ihc-testing-5.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IHC analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-ihc-testing-6.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IHC analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-ihc-testing-7.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IHC analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-ihc-testing-8.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IHC analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-if-testing-9.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) IF analysis of CPSF6 using anti-CPSF6 antibody (M04551). CPSF6 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-CPSF6 Antibody (M04551) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04551-cpsf6-primary-antibodies-fcm-testing-10.jpgAnti-CPSF6 Antibody Picoband® (monoclonal, 3F11E1) Flow Cytometry analysis of HepG2 cells using anti-CPSF6 antibody (M04551). Overlay histogram showing HepG2 cells stained with M04551 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CPSF6 Antibody (M04551, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ankyrin-erythroid-ank-ank1-picoband-trade-antibody-m02716-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02716-ank1-primary-antibodies-wb-testing-1.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 9I6C3) Western blot analysis of Ankyrin erythroid/ANK/ANK1 using anti-Ankyrin erythroid/ANK/ANK1 antibody (M02716). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ankyrin erythroid/ANK/ANK1 antigen affinity purified monoclonal antibody (Catalog # M02716) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ankyrin erythroid/ANK/ANK1 at approximately 206 kDa. The expected band size for Ankyrin erythroid/ANK/ANK1 is at 206 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ankyrin-erythroid-ank-ank1-picoband-trade-antibody-m02716-1-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02716-1-ank1-primary-antibodies-wb-testing-1.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 5H2E8) Western blot analysis of Ankyrin erythroid/ANK/ANK1 using anti-Ankyrin erythroid/ANK/ANK1 antibody (M02716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ankyrin erythroid/ANK/ANK1 antigen affinity purified monoclonal antibody (Catalog # M02716-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ankyrin erythroid/ANK/ANK1 at approximately 206 kDa. The expected band size for Ankyrin erythroid/ANK/ANK1 is at 206 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02716-1-ank1-primary-antibodies-fcm-testing-2.jpgAnti-Ankyrin erythroid/ANK/ANK1 Antibody Picoband® (monoclonal, 5H2E8) Flow Cytometry analysis of K562 cells using anti-Ankyrin erythroid/ANK/ANK1 antibody (M02716-1). Overlay histogram showing K562 cells stained with M02716-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ankyrin erythroid/ANK/ANK1 Antibody (M02716-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-me1-picoband-trade-antibody-m03449-boster.html2026-03-17T05:13:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03449-me1-primary-antibodies-wb-testing-1.jpgAnti-ME1 Antibody Picoband® (monoclonal, 5E5F7) Western blot analysis of ME1 using anti-ME1 antibody (M03449). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ME1 antigen affinity purified monoclonal antibody (Catalog # M03449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ME1 at approximately 64 kDa. The expected band size for ME1 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03449-me1-primary-antibodies-ihc-testing-2.jpgAnti-ME1 Antibody Picoband® (monoclonal, 5E5F7) IHC analysis of ME1 using anti-ME1 antibody (M03449). ME1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03449-me1-primary-antibodies-ihc-testing-3.jpgAnti-ME1 Antibody Picoband® (monoclonal, 5E5F7) IHC analysis of ME1 using anti-ME1 antibody (M03449). ME1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03449-me1-primary-antibodies-ihc-testing-4.jpgAnti-ME1 Antibody Picoband® (monoclonal, 5E5F7) IHC analysis of ME1 using anti-ME1 antibody (M03449). ME1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-ME1 Antibody (M03449) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-claudin-3-cldn3-picoband-trade-antibody-m04393-2-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04393-2-cldn3-primary-antibodies-wb-testing-1.jpgAnti-Claudin 3/CLDN3 Antibody Picoband® (monoclonal, 4C7D2) Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (M04393-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Claudin 3/CLDN3 antigen affinity purified monoclonal antibody (Catalog # M04393-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Claudin 3/CLDN3 at approximately 20 kDa. The expected band size for Claudin 3/CLDN3 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04393-2-cldn3-primary-antibodies-ihc-testing-2.jpgAnti-Claudin 3/CLDN3 Antibody Picoband® (monoclonal, 4C7D2) IHC analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (M04393-2). Claudin 3/CLDN3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Claudin 3/CLDN3 Antibody (M04393-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04393-2-cldn3-primary-antibodies-ihc-testing-3.jpgAnti-Claudin 3/CLDN3 Antibody Picoband® (monoclonal, 4C7D2) IHC analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (M04393-2). Claudin 3/CLDN3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Claudin 3/CLDN3 Antibody (M04393-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04393-2-cldn3-primary-antibodies-ihc-testing-4.jpgAnti-Claudin 3/CLDN3 Antibody Picoband® (monoclonal, 4C7D2) IHC analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (M04393-2). Claudin 3/CLDN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Claudin 3/CLDN3 Antibody (M04393-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04393-2-cldn3-primary-antibodies-if-testing-5.jpgAnti-Claudin 3/CLDN3 Antibody Picoband® (monoclonal, 4C7D2) IF analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (M04393-2). Claudin 3/CLDN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Claudin 3/CLDN3 Antibody (M04393-2) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pc4-sub1-picoband-trade-antibody-m02698-3-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-wb-testing-1.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PC4/SUB1 antigen affinity purified monoclonal antibody (Catalog # M02698-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 19 kDa. The expected band size for PC4/SUB1 is at 14 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-2.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-3.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-4.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-5.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-6.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-7.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-8.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-9.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-10.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-ihc-testing-11.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-fcm-testing-12.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) Flow Cytometry analysis of HepG2 cells using anti-PC4/SUB1 antibody (M02698-3). Overlay histogram showing HepG2 cells stained with M02698-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PC4/SUB1 Antibody (M02698-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02698-3-sub1-primary-antibodies-if-testing-13.jpgAnti-PC4/SUB1 Antibody Picoband® (monoclonal, 8D9D1) IF analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (M02698-3). PC4/SUB1 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-PC4/SUB1 Antibody (M02698-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calnexin-canx-picoband-trade-antibody-m03372-2-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03372-2-canx-primary-antibodies-wb-testing-1.jpgAnti-Calnexin/CANX Antibody Picoband® (monoclonal, 8D10B3) Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (M03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calnexin/CANX antigen affinity purified monoclonal antibody (Catalog # M03372-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 95 kDa. The expected band size for Calnexin/CANX is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03372-2-canx-primary-antibodies-ihc-testing-2.jpgAnti-Calnexin/CANX Antibody Picoband® (monoclonal, 8D10B3) IHC analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (M03372-2). Calnexin/CANX was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Calnexin/CANX Antibody (M03372-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03372-2-canx-primary-antibodies-ihc-testing-3.jpgAnti-Calnexin/CANX Antibody Picoband® (monoclonal, 8D10B3) IHC analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (M03372-2). Calnexin/CANX was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Calnexin/CANX Antibody (M03372-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03372-2-canx-primary-antibodies-ihc-testing-4.jpgAnti-Calnexin/CANX Antibody Picoband® (monoclonal, 8D10B3) IHC analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (M03372-2). Calnexin/CANX was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Calnexin/CANX Antibody (M03372-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03372-2-canx-primary-antibodies-ihc-testing-5.jpgAnti-Calnexin/CANX Antibody Picoband® (monoclonal, 8D10B3) IHC analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (M03372-2). Calnexin/CANX was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Calnexin/CANX Antibody (M03372-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03372-2-canx-primary-antibodies-fcm-testing-7.jpgAnti-Calnexin/CANX Antibody Picoband® (monoclonal, 8D10B3) Flow Cytometry analysis of A549 cells using anti-Calnexin/CANX antibody (M03372-2). Overlay histogram showing A549 cells stained with M03372-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Calnexin/CANX Antibody (M03372-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03372-2-canx-primary-antibodies-if-testing-6.jpgAnti-Calnexin/CANX Antibody Picoband® (monoclonal, 8D10B3) IF analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (M03372-2). Calnexin/CANX was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Calnexin/CANX Antibody (M03372-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glutathione-peroxidase-4-gpx4-picoband-trade-antibody-m02059-1-boster.html2026-03-25T05:24:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-1-gpx4-primary-antibodies-wb-testing-1.jpgAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7) Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (M02059-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified monoclonal antibody (Catalog # M02059-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 19 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-1-gpx4-primary-antibodies-fcm-testing-2.jpgAnti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (monoclonal, 6I4E7) Flow Cytometry analysis of Hela cells using anti-Glutathione Peroxidase 4/GPX4 antibody (M02059-1). Overlay histogram showing Hela cells stained with M02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutathione Peroxidase 4/GPX4 Antibody (M02059-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mdh2-picoband-trade-antibody-m04803-1-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04803-1-mdh2-primary-antibodies-wb-testing-1.jpgAnti-MDH2 Antibody Picoband® (monoclonal, 5D8C1) Western blot analysis of MDH2 using anti-MDH2 antibody (M04803-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MDH2 antigen affinity purified monoclonal antibody (Catalog # M04803-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MDH2 at approximately 36 kDa. The expected band size for MDH2 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04803-1-mdh2-primary-antibodies-ihc-testing-2.jpgAnti-MDH2 Antibody Picoband® (monoclonal, 5D8C1) IHC analysis of MDH2 using anti-MDH2 antibody (M04803-1). MDH2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MDH2 Antibody (M04803-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04803-1-mdh2-primary-antibodies-ihc-testing-3.jpgAnti-MDH2 Antibody Picoband® (monoclonal, 5D8C1) IHC analysis of MDH2 using anti-MDH2 antibody (M04803-1). MDH2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MDH2 Antibody (M04803-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04803-1-mdh2-primary-antibodies-ihc-testing-4.jpgAnti-MDH2 Antibody Picoband® (monoclonal, 5D8C1) IHC analysis of MDH2 using anti-MDH2 antibody (M04803-1). MDH2 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-MDH2 Antibody (M04803-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04803-1-mdh2-primary-antibodies-if-testing-5.jpgAnti-MDH2 Antibody Picoband® (monoclonal, 5D8C1) IF analysis of MDH2 using anti-MDH2 antibody (M04803-1). MDH2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-MDH2 Antibody (M04803-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lyric-picoband-trade-antibody-m04060-2-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04060-2-lyric-primary-antibodies-wb-testing-1.jpgAnti-LYRIC Antibody Picoband® (monoclonal, 2E5G3) Western blot analysis of LYRIC using anti-LYRIC antibody (M04060-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-LYRIC antigen affinity purified monoclonal antibody (Catalog # M04060-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for LYRIC at approximately 85 kDa. The expected band size for LYRIC is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04060-2-lyric-primary-antibodies-ihc-testing-2.jpgAnti-LYRIC Antibody Picoband® (monoclonal, 2E5G3) IHC analysis of LYRIC using anti-LYRIC antibody (M04060-2). LYRIC was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-LYRIC Antibody (M04060-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04060-2-lyric-primary-antibodies-fcm-testing-3.jpgAnti-LYRIC Antibody Picoband® (monoclonal, 2E5G3) Flow Cytometry analysis of U87 cells using anti-LYRIC antibody (M04060-2). Overlay histogram showing U87 cells stained with M04060-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LYRIC Antibody (M04060-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ndp52-calcoco2-picoband-trade-antibody-m05876-2-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05876-2-calcoco2-primary-antibodies-wb-testing-1.jpgAnti-NDP52/CALCOCO2 Antibody Picoband® (monoclonal, 9E2F2) Western blot analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (M05876-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NDP52/CALCOCO2 antigen affinity purified monoclonal antibody (Catalog # M05876-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NDP52/CALCOCO2 at approximately 52 kDa. The expected band size for NDP52/CALCOCO2 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05876-2-calcoco2-primary-antibodies-if-testing-2.jpgAnti-NDP52/CALCOCO2 Antibody Picoband® (monoclonal, 9E2F2) IF analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (M05876-2). NDP52/CALCOCO2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-NDP52/CALCOCO2 Antibody (M05876-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05876-2-calcoco2-primary-antibodies-fcm-testing-3.jpgAnti-NDP52/CALCOCO2 Antibody Picoband® (monoclonal, 9E2F2) Flow Cytometry analysis of MCF-7 cells using anti-NDP52/CALCOCO2 antibody (M05876-2). Overlay histogram showing MCF-7 cells stained with M05876-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NDP52/CALCOCO2 Antibody (M05876-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gamma-catenin-picoband-trade-antibody-m01901-3-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01901-3-gamma-catenin-primary-antibodies-wb-testing-1.jpgAnti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7) Western blot analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-gamma Catenin antigen affinity purified monoclonal antibody (Catalog # M01901-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for gamma Catenin at approximately 82 kDa. The expected band size for gamma Catenin is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01901-3-gamma-catenin-primary-antibodies-ihc-testing-2.jpgAnti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7) IHC analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3). gamma Catenin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-gamma Catenin Antibody (M01901-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01901-3-gamma-catenin-primary-antibodies-ihc-testing-3.jpgAnti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7) IHC analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3). gamma Catenin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-gamma Catenin Antibody (M01901-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01901-3-gamma-catenin-primary-antibodies-if-testing-4.jpgAnti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7) IF analysis of gamma Catenin using anti-gamma Catenin antibody (M01901-3). gamma Catenin was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-gamma Catenin Antibody (M01901-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01901-3-gamma-catenin-primary-antibodies-fcm-testing-5.jpgAnti-gamma Catenin Antibody Picoband® (monoclonal, 4C12D7) Flow Cytometry analysis of MCF-7 cells using anti-gamma Catenin antibody (M01901-3). Overlay histogram showing MCF-7 cells stained with M01901-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-gamma Catenin Antibody (M01901-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klc1-picoband-trade-antibody-m04116-1-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04116-1-klc1-primary-antibodies-wb-testing-1.jpgAnti-KLC1 Antibody Picoband® (monoclonal, 4F2D7) Western blot analysis of KLC1 using anti-KLC1 antibody (M04116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KLC1 antigen affinity purified monoclonal antibody (Catalog # M04116-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KLC1 at approximately 65 kDa. The expected band size for KLC1 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cbs-picoband-trade-antibody-m00130-2-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00130-2-cbs-primary-antibodies-wb-testing-1.jpgAnti-CBS Antibody Picoband® (monoclonal, 7C3B7) Western blot analysis of CBS using anti-CBS antibody (M00130-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CBS antigen affinity purified monoclonal antibody (Catalog # M00130-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CBS at approximately 61 kDa. The expected band size for CBS is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cbs-picoband-trade-antibody-m00130-3-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00130-3-cbs-primary-antibodies-wb-testing-1.jpgAnti-CBS Antibody Picoband® (monoclonal, 5B5D7) Western blot analysis of CBS using anti-CBS antibody (M00130-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CBS antigen affinity purified monoclonal antibody (Catalog # M00130-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CBS at approximately 61 kDa. The expected band size for CBS is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00130-3-cbs-primary-antibodies-fcm-testing-2.jpgAnti-CBS Antibody Picoband® (monoclonal, 5B5D7) Flow Cytometry analysis of MCF-7 cells using anti-CBS antibody (M00130-3). Overlay histogram showing MCF-7 cells stained with M00130-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CBS Antibody (M00130-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aconitase-2-picoband-trade-antibody-m03096-3-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-wb-testing-1.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) Western blot analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Aconitase 2 antigen affinity purified monoclonal antibody (Catalog # M03096-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Aconitase 2 at approximately 85 kDa. The expected band size for Aconitase 2 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-ihc-testing-2.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Aconitase 2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-ihc-testing-3.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Aconitase 2 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-ihc-testing-4.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Aconitase 2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-ihc-testing-5.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Aconitase 2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-ihc-testing-6.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Aconitase 2 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-ihc-testing-7.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Aconitase 2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03096-3-aco2-primary-antibodies-ihc-testing-8.jpgAnti-Aconitase 2 Antibody Picoband® (monoclonal, 4C12D1) IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (M03096-3). Aconitase 2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aconitase 2 Antibody (M03096-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-xiap-picoband-trade-antibody-m00482-1-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00482-1-xiap-primary-antibodies-wb-testing-1.jpgAnti-XIAP Antibody Picoband® (monoclonal, 3G2G1) Western blot analysis of XIAP using anti-XIAP antibody (M00482-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-XIAP antigen affinity purified monoclonal antibody (Catalog # M00482-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for XIAP at approximately 54 kDa. The expected band size for XIAP is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-angiopoietin-like-4-angptl4-picoband-trade-antibody-a01147-2-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01147-2-angptl4-primary-antibodies-wb-testing-1.jpgAnti-Angiopoietin-like 4/ANGPTL4 Antibody Picoband® Western blot analysis of Angiopoietin-like 4/ANGPTL4 using anti-Angiopoietin-like 4/ANGPTL4 antibody (A01147-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-like 4/ANGPTL4 antigen affinity purified polyclonal antibody (Catalog # A01147-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-like 4/ANGPTL4 at approximately 45 kDa. The expected band size for Angiopoietin-like 4/ANGPTL4 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-annexin-a10-anxa10-picoband-trade-antibody-a13560-1-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13560-1-anxa10-primary-antibodies-wb-testing-1.jpgAnti-Annexin A10/ANXA10 Antibody Picoband® Western blot analysis of Annexin A10/ANXA10 using anti-Annexin A10/ANXA10 antibody (A13560-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A10/ANXA10 antigen affinity purified polyclonal antibody (Catalog # A13560-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A10/ANXA10 at approximately 36 kDa. The expected band size for Annexin A10/ANXA10 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13560-1-anxa10-primary-antibodies-ihc-testing-2.jpgAnti-Annexin A10/ANXA10 Antibody Picoband® IHC analysis of Annexin A10/ANXA10 using anti-Annexin A10/ANXA10 antibody (A13560-1). Annexin A10/ANXA10 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13560-1-anxa10-primary-antibodies-ihc-testing-3.jpgAnti-Annexin A10/ANXA10 Antibody Picoband® IHC analysis of Annexin A10/ANXA10 using anti-Annexin A10/ANXA10 antibody (A13560-1). Annexin A10/ANXA10 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13560-1-anxa10-primary-antibodies-ihc-testing-4.jpgAnti-Annexin A10/ANXA10 Antibody Picoband® IHC analysis of Annexin A10/ANXA10 using anti-Annexin A10/ANXA10 antibody (A13560-1). Annexin A10/ANXA10 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13560-1-anxa10-primary-antibodies-ihc-testing-5.jpgAnti-Annexin A10/ANXA10 Antibody Picoband® IHC analysis of Annexin A10/ANXA10 using anti-Annexin A10/ANXA10 antibody (A13560-1). Annexin A10/ANXA10 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13560-1-anxa10-primary-antibodies-if-testing-6.jpgAnti-Annexin A10/ANXA10 Antibody Picoband® IF analysis of Annexin A10/ANXA10 using anti-Annexin A10/ANXA10 antibody (A13560-1). Annexin A10/ANXA10 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13560-1-anxa10-primary-antibodies-fcm-testing-7.jpgAnti-Annexin A10/ANXA10 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-Annexin A10/ANXA10 antibody (A13560-1). Overlay histogram showing PC-3 cells stained with A13560-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin A10/ANXA10 Antibody (A13560-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-apobec3b-picoband-trade-antibody-a01088-1-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01088-1-apobec3b-primary-antibodies-wb-testing-1_1.jpgAnti-APOBEC3B Antibody Picoband® Western blot analysis of APOBEC3B using anti-APOBEC3B antibody (A01088-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3B antigen affinity purified polyclonal antibody (Catalog # A01088-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3B at approximately 35 kDa. The expected band size for APOBEC3B is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-amphiregulin-areg-picoband-trade-antibody-a01787-2-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01787-2-areg-primary-antibodies-wb-testing-1.jpgAnti-Amphiregulin/AREG Antibody Picoband® Western blot analysis of Amphiregulin/AREG using anti-Amphiregulin/AREG antibody (A01787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin/AREG antigen affinity purified polyclonal antibody (Catalog # A01787-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin/AREG at approximately 45 kDa. The expected band size for Amphiregulin/AREG is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01787-2-areg-primary-antibodies-fcm-testing-2_1.jpgAnti-Amphiregulin/AREG Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Amphiregulin/AREG antibody (A01787-2). Overlay histogram showing HepG2 cells stained with A01787-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Amphiregulin/AREG Antibody (A01787-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-arl1-picoband-trade-antibody-a03733-1-boster.html2026-03-17T05:13:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03733-1-arl1-primary-antibodies-wb-testing-1.jpgAnti-ARL1 Antibody Picoband® Western blot analysis of ARL1 using anti-ARL1 antibody (A03733-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL1 antigen affinity purified polyclonal antibody (Catalog # A03733-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL1 at approximately 20 kDa. The expected band size for ARL1 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03733-1-arl1-primary-antibodies-ihc-testing-2.jpgAnti-ARL1 Antibody Picoband® IHC analysis of ARL1 using anti-ARL1 antibody (A03733-1). ARL1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARL1 Antibody (A03733-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03733-1-arl1-primary-antibodies-ihc-testing-3.jpgAnti-ARL1 Antibody Picoband® IHC analysis of ARL1 using anti-ARL1 antibody (A03733-1). ARL1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARL1 Antibody (A03733-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03733-1-arl1-primary-antibodies-if-testing-4.jpgAnti-ARL1 Antibody Picoband® IF analysis of ARL1 using anti-ARL1 antibody (A03733-1). ARL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ARL1 Antibody (A03733-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03733-1-arl1-primary-antibodies-fcm-testing-5.jpgAnti-ARL1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ARL1 antibody (A03733-1). Overlay histogram showing RT4 cells stained with A03733-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARL1 Antibody (A03733-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-puma-bbc3-picoband-trade-antibody-a04899-3-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04899-3-bbc3-primary-antibodies-wb-testing-1.jpgAnti-PUMA/BBC3 Antibody Picoband® Western blot analysis of PUMA/BBC3 using anti-PUMA/BBC3 antibody (A04899-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUMA/BBC3 antigen affinity purified polyclonal antibody (Catalog # A04899-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUMA/BBC3 at approximately 18 kDa. The expected band size for PUMA/BBC3 is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-dup-cdt1-picoband-trade-antibody-a01035-2-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01035-2-cdt1-primary-antibodies-wb-testing-1.jpgAnti-DUP/CDT1 Antibody Picoband® Western blot analysis of DUP/CDT1 using anti-DUP/CDT1 antibody (A01035-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUP/CDT1 antigen affinity purified polyclonal antibody (Catalog # A01035-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUP/CDT1 at approximately 60 kDa. The expected band size for DUP/CDT1 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-clasp1-picoband-trade-antibody-a04813-3-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-wb-testing-1.jpgAnti-CLASP1 Antibody Picoband® Western blot analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLASP1 antigen affinity purified polyclonal antibody (Catalog # A04813-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLASP1 at approximately 150 kDa. The expected band size for CLASP1 is at 150 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-ihc-testing-2.jpgAnti-CLASP1 Antibody Picoband® IHC analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). CLASP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CLASP1 Antibody (A04813-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-ihc-testing-3.jpgAnti-CLASP1 Antibody Picoband® IHC analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). CLASP1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CLASP1 Antibody (A04813-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-ihc-testing-4.jpgAnti-CLASP1 Antibody Picoband® IHC analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). CLASP1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CLASP1 Antibody (A04813-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-ihc-testing-5.jpgAnti-CLASP1 Antibody Picoband® IHC analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). CLASP1 was detected in a paraffin-embedded section of human squamous cell carcinoma of cervix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CLASP1 Antibody (A04813-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-ihc-testing-6.jpgAnti-CLASP1 Antibody Picoband® IHC analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). CLASP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CLASP1 Antibody (A04813-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-if-testing-7.jpgAnti-CLASP1 Antibody Picoband® IF analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). CLASP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CLASP1 Antibody (A04813-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04813-3-clasp1-primary-antibodies-if-testing-8.jpgAnti-CLASP1 Antibody Picoband® IF analysis of CLASP1 using anti-CLASP1 antibody (A04813-3). CLASP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CLASP1 Antibody (A04813-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-glis1-picoband-trade-antibody-a12698-1-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-wb-testing-1.jpgAnti-GLIS1 Antibody Picoband® Western blot analysis of GLIS1 using anti-GLIS1 antibody (A12698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLIS1 antigen affinity purified polyclonal antibody (Catalog # A12698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLIS1 at approximately 66 kDa. The expected band size for GLIS1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-ihc-testing-2.jpgAnti-GLIS1 Antibody Picoband® IHC analysis of GLIS1 using anti-GLIS1 antibody (A12698-1). GLIS1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLIS1 Antibody (A12698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-ihc-testing-3.jpgAnti-GLIS1 Antibody Picoband® IHC analysis of GLIS1 using anti-GLIS1 antibody (A12698-1). GLIS1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLIS1 Antibody (A12698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-ihc-testing-4.jpgAnti-GLIS1 Antibody Picoband® IHC analysis of GLIS1 using anti-GLIS1 antibody (A12698-1). GLIS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLIS1 Antibody (A12698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-ihc-testing-5.jpgAnti-GLIS1 Antibody Picoband® IHC analysis of GLIS1 using anti-GLIS1 antibody (A12698-1). GLIS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLIS1 Antibody (A12698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-ihc-testing-6.jpgAnti-GLIS1 Antibody Picoband® IHC analysis of GLIS1 using anti-GLIS1 antibody (A12698-1). GLIS1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLIS1 Antibody (A12698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-if-testing-7.jpgAnti-GLIS1 Antibody Picoband® IF analysis of GLIS1 using anti-GLIS1 antibody (A12698-1). GLIS1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GLIS1 Antibody (A12698-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12698-1-glis1-primary-antibodies-fcm-testing-8.jpgAnti-GLIS1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-GLIS1 antibody (A12698-1). Overlay histogram showing 293T cells stained with A12698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLIS1 Antibody (A12698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-glis3-picoband-trade-antibody-a03710-3-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03710-3-glis3-primary-antibodies-wb-testing-1.jpgAnti-GLIS3 Antibody Picoband® Western blot analysis of GLIS3 using anti-GLIS3 antibody (A03710-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLIS3 antigen affinity purified polyclonal antibody (Catalog # A03710-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLIS3 at approximately 84 kDa. The expected band size for GLIS3 is at 84 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-grb2-picoband-trade-antibody-a00351-1-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00351-1-grb2-primary-antibodies-wb-testing-1.jpgAnti-GRB2 Antibody Picoband® Western blot analysis of GRB2 using anti-GRB2 antibody (A00351-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (Catalog # A00351-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25 kDa. The expected band size for GRB2 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00351-1-grb2-primary-antibodies-fcm-testing-2.jpgAnti-GRB2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-GRB2 antibody (A00351-1). Overlay histogram showing PC-3 cells stained with A00351-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB2 Antibody (A00351-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hdac1-picoband-trade-antibody-a00256-4-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-4-hdac1-primary-antibodies-wb-testing-1.jpgAnti-HDAC1 Antibody Picoband® Western blot analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC1 antigen affinity purified polyclonal antibody (Catalog # A00256-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC1 at approximately 65 kDa. The expected band size for HDAC1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-4-hdac1-primary-antibodies-ihc-testing-2.jpgAnti-HDAC1 Antibody Picoband® IHC analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). HDAC1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC1 Antibody (A00256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-4-hdac1-primary-antibodies-ihc-testing-3.jpgAnti-HDAC1 Antibody Picoband® IHC analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). HDAC1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC1 Antibody (A00256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-4-hdac1-primary-antibodies-ihc-testing-4.jpgAnti-HDAC1 Antibody Picoband® IHC analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). HDAC1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC1 Antibody (A00256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-4-hdac1-primary-antibodies-fcm-testing-5.jpgAnti-HDAC1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-HDAC1 antibody (A00256-4). Overlay histogram showing MCF-7 cells stained with A00256-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC1 Antibody (A00256-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-heme-oxygenase-1-hmox1-picoband-trade-antibody-a00253-1-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00253-1-hmox1-primary-antibodies-wb-testing-1.jpgAnti-Heme Oxygenase 1/Hmox1 Antibody Picoband® Western blot analysis of Heme Oxygenase 1/Hmox1 using anti-Heme Oxygenase 1/Hmox1 antibody (A00253-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/Hmox1 antigen affinity purified polyclonal antibody (Catalog # A00253-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/Hmox1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/Hmox1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00253-1-12906_2019_2755_fig5_html.pngAnti-Heme Oxygenase 1/Hmox1 Antibody Picoband®Effects of CSSPW on the expression of Nrf2/HO-1 pathway in LPS-induced RAW264.7 cells. Cells were incubated with CSSPW (15 ng/mL, 1.5 μg/mL and 150 μg/mL), Dex (0.13 mg/mL) and LPS (1 μg/mL) for 24 h. a and c Western blotting results of Nrf2 and HO-1 proteins. Graphs represented relative expression of Nrf2 ( b ) and HO-1 ( d ). Data were represented as mean ± SD ( n = 3); ** p < 0.01 vs. the Con group; ## p < 0.01 vs. the LPS group; $$ p < 0.01 vs. the 15 ng/mL group Index in PubMed under a CC BY license. PMID: <a href='https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-019-2755-6'>31791318</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00253-1-ijbms-21-1238-g005.jpgAnti-Heme Oxygenase 1/Hmox1 Antibody Picoband®Tempol further promoted intermittent hypoxia-induced activation of the Nrf2/HO-1 signaling pathway. (A) The level of HO-1 in lung tissues was detected by a commercial kit. (B) The protein levels of HO-1, cytoplasmic Nrf2, and nuclear Nrf2 in lung tissues were measured by Western blot assay. GAPDH was used as a loading control. (C)& (D) The gray-scale value of the bands was quantitatively analyzed. (E) DNA binding activity of Nrf2 in lung tissues was determined by EMSA. (F) Quantitative analysis of Nrf2 DNA binding activity. The experimental data were expressed as mean±SD (n=6). * P< 0.05, *** P< 0.001, versus the NA group. ### P< 0.001, versus the IH group. ns, no significance, versus the NA group Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6312670/&orig_host=www.ncbi.nlm.nih.gov'>30627367</a> https://www.bosterbio.com/products/primary-antibodies/anti-hoxa4-picoband-trade-antibody-a07948-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07948-hoxa4-primary-antibodies-wb-testing-1.jpgAnti-HOXA4 Antibody Picoband® Western blot analysis of HOXA4 using anti-HOXA4 antibody (A07948). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA4 antigen affinity purified polyclonal antibody (Catalog # A07948) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA4 at approximately 36 kDa. The expected band size for HOXA4 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-hoxc4-picoband-trade-antibody-a09869-1-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-wb-testing-1.jpgAnti-HOXC4 Antibody Picoband® Western blot analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC4 antigen affinity purified polyclonal antibody (Catalog # A09869-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXC4 at approximately 36 kDa. The expected band size for HOXC4 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-ihc-testing-2.jpgAnti-HOXC4 Antibody Picoband® IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-ihc-testing-3.jpgAnti-HOXC4 Antibody Picoband® IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-ihc-testing-4.jpgAnti-HOXC4 Antibody Picoband® IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-ihc-testing-5.jpgAnti-HOXC4 Antibody Picoband® IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-ihc-testing-6.jpgAnti-HOXC4 Antibody Picoband® IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-ihc-testing-7.jpgAnti-HOXC4 Antibody Picoband® IHC analysis of HOXC4 using anti-HOXC4 antibody (A09869-1). HOXC4 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXC4 Antibody (A09869-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-fcm-testing-8.jpgAnti-HOXC4 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-HOXC4 antibody (A09869-1). Overlay histogram showing A431 cells stained with A09869-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXC4 Antibody (A09869-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09869-1-hoxc4-primary-antibodies-fcm-testing-9.jpgAnti-HOXC4 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-HOXC4 antibody (A09869-1). Overlay histogram showing RH35 cells stained with A09869-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXC4 Antibody (A09869-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hsd17b3-picoband-trade-antibody-a04128-3-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04128-3-hsd17b3-primary-antibodies-wb-testing-1.jpgAnti-HSD17B3 Antibody Picoband® Western blot analysis of HSD17B3 using anti-HSD17B3 antibody (A04128-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B3 antigen affinity purified polyclonal antibody (Catalog # A04128-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B3 at approximately 40 kDa. The expected band size for HSD17B3 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04128-3-hsd17b3-primary-antibodies-fcm-testing-2.jpgAnti-HSD17B3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-HSD17B3 antibody (A04128-3). Overlay histogram showing HepG2 cells stained with A04128-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B3 Antibody (A04128-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hsf1-picoband-trade-antibody-a00250-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00250-hsf1-primary-antibodies-wb-testing-1.jpgAnti-HSF1 Antibody Picoband® Western blot analysis of HSF1 using anti-HSF1 antibody (A00250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 antigen affinity purified polyclonal antibody (Catalog # A00250) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF1 at approximately 80 kDa. The expected band size for HSF1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00250-hsf1-primary-antibodies-ihc-testing-2.jpgAnti-HSF1 Antibody Picoband® IHC analysis of HSF1 using anti-HSF1 antibody (A00250). HSF1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSF1 Antibody (A00250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00250-hsf1-primary-antibodies-ihc-testing-3.jpgAnti-HSF1 Antibody Picoband® IHC analysis of HSF1 using anti-HSF1 antibody (A00250). HSF1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSF1 Antibody (A00250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00250-hsf1-primary-antibodies-ihc-testing-4.jpgAnti-HSF1 Antibody Picoband® IHC analysis of HSF1 using anti-HSF1 antibody (A00250). HSF1 was detected in a paraffin-embedded section of human squamous cell carcinoma of cervix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSF1 Antibody (A00250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00250-hsf1-primary-antibodies-ihc-testing-5.jpgAnti-HSF1 Antibody Picoband® IHC analysis of HSF1 using anti-HSF1 antibody (A00250). HSF1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSF1 Antibody (A00250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00250-hsf1-primary-antibodies-fcm-testing-6.jpgAnti-HSF1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-HSF1 antibody (A00250). Overlay histogram showing MCF-7 cells stained with A00250 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSF1 Antibody (A00250, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-il-10-il10-picoband-trade-antibody-a00021-4-boster.html2026-03-17T05:13:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00021-4-il10-primary-antibodies-ihc-testing-1.jpgAnti-IL-10/IL10 Antibody IHC analysis of IL-10/IL10 using anti-IL-10/IL10 antibody (A00021-4). IL-10/IL10 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-10/IL10 Antibody (A00021-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-il17a-picoband-trade-antibody-a00421-4-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00421-4-il17a-primary-antibodies-ihc-testing-1.jpgAnti-IL17A Antibody IHC analysis of IL17A using anti-IL17A antibody (A00421-4). IL17A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL17A Antibody (A00421-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-impdh2-picoband-trade-antibody-a03021-2-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-wb-testing-1.jpgAnti-IMPDH2 Antibody Picoband® Western blot analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMPDH2 antigen affinity purified polyclonal antibody (Catalog # A03021-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMPDH2 at approximately 56 kDa. The expected band size for IMPDH2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-wb-testing-2.jpgAnti-IMPDH2 Antibody Picoband®Western blot analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancrease tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMPDH2 antigen affinity purified polyclonal antibody (Catalog # A03021-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IMPDH2 at approximately 56 kDa. The expected band size for IMPDH2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-ihc-testing-2.jpgAnti-IMPDH2 Antibody Picoband® IHC analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-ihc-testing-3.jpgAnti-IMPDH2 Antibody Picoband® IHC analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-ihc-testing-4.jpgAnti-IMPDH2 Antibody Picoband® IHC analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-ihc-testing-5.jpgAnti-IMPDH2 Antibody Picoband® IHC analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-if-testing-6.jpgAnti-IMPDH2 Antibody Picoband® IF analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-ihc-testing-6.jpgAnti-IMPDH2 Antibody Picoband®IHC analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-ihc-testing-7.jpgAnti-IMPDH2 Antibody Picoband®IHC analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-if-testing-7.jpgAnti-IMPDH2 Antibody Picoband® IF analysis of IMPDH2 using anti-IMPDH2 antibody (A03021-2). IMPDH2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-IMPDH2 Antibody (A03021-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03021-2-impdh2-primary-antibodies-fcm-testing-8.jpgAnti-IMPDH2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-IMPDH2 antibody (A03021-2). Overlay histogram showing U20S cells stained with A03021-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IMPDH2 Antibody (A03021-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mars1-picoband-trade-antibody-a00216-1-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00216-1-mars1-primary-antibodies-wb-testing-1_1.jpgAnti-MARS1 Antibody Picoband®Western blot analysis of MARS1 using anti-MARS1 antibody (A00216-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARS1 antigen affinity purified polyclonal antibody (A00216-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MARS1 at approximately 101 kDa. The expected band size for MARS1 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00216-1-mars1-primary-antibodies-ihc-testing-1.jpgAnti-MARS1 Antibody Picoband®IHC analysis of MARS1 using anti-MARS1 antibody (A00216-1). MARS1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARS1 Antibody (A00216-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00216-1-mars1-primary-antibodies-ihc-testing-2.jpgAnti-MARS1 Antibody Picoband®IHC analysis of MARS1 using anti-MARS1 antibody (A00216-1). MARS1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARS1 Antibody (A00216-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00216-1-mars1-primary-antibodies-if-testing-1.jpgAnti-MARS1 Antibody Picoband®IF analysis of MARS1 using anti-MARS1 antibody (A00216-1). MARS1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MARS1 Antibody (A00216-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00216-1-mars1-primary-antibodies-if-testing-2.jpgAnti-MARS1 Antibody Picoband®IF analysis of MARS1 using anti-MARS1 antibody (A00216-1). MARS1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MARS1 Antibody (A00216-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00216-1-mars1-primary-antibodies-ip-testing-1.jpgAnti-MARS1 Antibody Picoband®Immunoprecipitating (IP) MARS1 in A549 whole cell lysate. Western blot analysis of MARS1 using anti-MARS1 antibody (A00216-1); Lane 1: A549 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-MARS1 antibody in A549 whole cell lysate; Lane 3: anti-MARS1 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MARS1 antigen affinity purified polyclonal antibody (A00216-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MARS1 at approximately 101 kDa. The expected band size for MARS1 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00216-1-mars1-primary-antibodies-fcm-testing-1.jpgAnti-MARS1 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-MARS1 antibody (A00216-1). Overlay histogram showing K562 cells stained with A00216-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MARS1 Antibody (A00216-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mlx-picoband-trade-antibody-a03087-3-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03087-3-mlx-primary-antibodies-wb-testing-1.jpgAnti-MLX Antibody Picoband® Western blot analysis of MLX using anti-MLX antibody (A03087-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX antigen affinity purified polyclonal antibody (Catalog # A03087-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLX at approximately 33 kDa. The expected band size for MLX is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-aag-mpg-picoband-trade-antibody-a00037-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00037-mpg-primary-antibodies-wb-testing-1.jpgAnti-AAG/MPG Antibody Picoband® Western blot analysis of AAG/MPG using anti-AAG/MPG antibody (A00037). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAG/MPG antigen affinity purified polyclonal antibody (Catalog # A00037) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AAG/MPG at approximately 37 kDa. The expected band size for AAG/MPG is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-mthfd1-picoband-trade-antibody-a02025-1-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-wb-testing-1.jpgAnti-MTHFD1 Antibody Picoband® Western blot analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD1 antigen affinity purified polyclonal antibody (Catalog # A02025-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTHFD1 at approximately 102 kDa. The expected band size for MTHFD1 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-2.jpgAnti-MTHFD1 Antibody Picoband® IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in a paraffin-embedded section of human adenocarcinoma of the lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-3.jpgAnti-MTHFD1 Antibody Picoband® IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-4.jpgAnti-MTHFD1 Antibody Picoband® IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-5.jpgAnti-MTHFD1 Antibody Picoband® IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-6.jpgAnti-MTHFD1 Antibody Picoband® IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-7.jpgAnti-MTHFD1 Antibody Picoband® IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-if-testing-8.jpgAnti-MTHFD1 Antibody Picoband® IF analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-fcm-testing-10.jpgAnti-MTHFD1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-MTHFD1 antibody (A02025-1). Overlay histogram showing JK cells stained with A02025-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTHFD1 Antibody (A02025-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02025-1-mthfd1-primary-antibodies-if-testing-9.jpgAnti-MTHFD1 Antibody Picoband® IF analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1). MTHFD1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ndufv2-picoband-trade-antibody-a05801-2-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05801-2-ndufv2-primary-antibodies-wb-testing-1.jpgAnti-NDUFV2 Antibody Picoband® Western blot analysis of NDUFV2 using anti-NDUFV2 antibody (A05801-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFV2 antigen affinity purified polyclonal antibody (Catalog # A05801-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFV2 at approximately 27 kDa. The expected band size for NDUFV2 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05801-2-ndufv2-primary-antibodies-ihc-testing-2.jpgAnti-NDUFV2 Antibody Picoband® IHC analysis of NDUFV2 using anti-NDUFV2 antibody (A05801-2). NDUFV2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFV2 Antibody (A05801-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05801-2-ndufv2-primary-antibodies-ihc-testing-3.jpgAnti-NDUFV2 Antibody Picoband® IHC analysis of NDUFV2 using anti-NDUFV2 antibody (A05801-2). NDUFV2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFV2 Antibody (A05801-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05801-2-ndufv2-primary-antibodies-ihc-testing-4.jpgAnti-NDUFV2 Antibody Picoband® IHC analysis of NDUFV2 using anti-NDUFV2 antibody (A05801-2). NDUFV2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFV2 Antibody (A05801-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05801-2-ndufv2-primary-antibodies-if-testing-5.jpgAnti-NDUFV2 Antibody Picoband® IF analysis of NDUFV2 using anti-NDUFV2 antibody (A05801-2). NDUFV2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFV2 Antibody (A05801-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05801-2-ndufv2-primary-antibodies-fcm-testing-6.jpgAnti-NDUFV2 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-NDUFV2 antibody (A05801-2). Overlay histogram showing A549 cells stained with A05801-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFV2 Antibody (A05801-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ikb-epsilon-nfkbie-picoband-trade-antibody-a07073-1-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07073-1-nfkbie-primary-antibodies-wb-testing-1.jpgAnti-IKB epsilon/Nfkbie Antibody Picoband® Western blot analysis of IKB epsilon/Nfkbie using anti-IKB epsilon/Nfkbie antibody (A07073-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB epsilon/Nfkbie antigen affinity purified polyclonal antibody (Catalog # A07073-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKB epsilon/Nfkbie at approximately 41 kDa. The expected band size for IKB epsilon/Nfkbie is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-nrbf2-picoband-trade-antibody-a09144-2-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09144-2-nrbf2-primary-antibodies-wb-testing-1.jpgAnti-NRBF2 Antibody Picoband® Western blot analysis of NRBF2 using anti-NRBF2 antibody (A09144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRBF2 antigen affinity purified polyclonal antibody (Catalog # A09144-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRBF2 at approximately 38 kDa. The expected band size for NRBF2 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09144-2-nrbf2-primary-antibodies-fcm-testing-2.jpgAnti-NRBF2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-NRBF2 antibody (A09144-2). Overlay histogram showing U20S cells stained with A09144-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRBF2 Antibody (A09144-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rhno1-picoband-trade-antibody-a13755-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13755-rhno1-primary-antibodies-wb-testing-1.jpgAnti-RHNO1 Antibody Picoband® Western blot analysis of RHNO1 using anti-RHNO1 antibody (A13755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHNO1 antigen affinity purified polyclonal antibody (Catalog # A13755) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHNO1 at approximately 40 kDa. The expected band size for RHNO1 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13755-rhno1-primary-antibodies-fcm-testing-2.jpgAnti-RHNO1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-RHNO1 antibody (A13755). Overlay histogram showing RT4 cells stained with A13755 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RHNO1 Antibody (A13755, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rpl32-picoband-trade-antibody-a06487-2-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-wb-testing-1.jpgAnti-RPL32 Antibody Picoband® Western blot analysis of RPL32 using anti-RPL32 antibody (A06487-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL32 antigen affinity purified polyclonal antibody (Catalog # A06487-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL32 at approximately 18 kDa. The expected band size for RPL32 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-gr2_lrg.jpgAnti-RPL32 Antibody Picoband®(A) Schematic diagram for the workflow of platelet proteomics. (B) Principal-component analysis (PCA) plot showing the dimensionality reduction of the proteomics of mouse embryonic platelets (n = 3) and adult platelets (n = 3). (C) Heatmap showing the relative expression values (scaled expression) of differentially expressed proteins between embryonic and adult platelets. In embryonic platelets, 325 proteins were upregulated (padj < 0.05, log2FoldChange > log2(1.5)) and 211 proteins were downregulated (padj <0.05, log2FoldChange < −log2(1.5)). (D) Expression validation using intracellular flow cytometry for the representative proteins that were upregulated or downregulated in embryonic platelets (n = 3). ∗p < 0.05 and ∗∗p < 0.01. (E) Bar plot showing the representative biological processes upregulated (red) and downregulated (blue) in embryonic platelet proteomics. The x axis represents the statistical significance, −log10(adjusted p). (F) Boxplot showing the average expression levels of proteins related to representative differentially enriched biological processes between embryonic and adult platelet proteomics. (G) Heatmap showing the relative expression levels of representative multi-organ development-related proteins that are upregulated in the embryonic proteomics. Genes are sorted by fold change from high to low. (H) Heatmap showing the relative expression levels of representative immune-related proteins that are downregulated in the embryonic proteomics. Genes are sorted by fold change from high to low. Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/cell-reports-medicine/fulltext/S2666-3791(25)00370-2'>40795844</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-wb-testing-2.jpgAnti-RPL32 Antibody Picoband® Western blot analysis of RPL32 using anti-RPL32 antibody (A06487-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat pancreas tissue lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL32 antigen affinity purified polyclonal antibody (Catalog # A06487-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL32 at approximately 18 kDa. The expected band size for RPL32 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-ihc-testing-3.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-2). RPL32 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-ihc-testing-4.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-2). RPL32 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-ihc-testing-5.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-2). RPL32 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-ihc-testing-6.jpgAnti-RPL32 Antibody Picoband® IHC analysis of RPL32 using anti-RPL32 antibody (A06487-2). RPL32 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL32 Antibody (A06487-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-if-testing-7.jpgAnti-RPL32 Antibody Picoband® IF analysis of RPL32 using anti-RPL32 antibody (A06487-2). RPL32 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPL32 Antibody (A06487-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-if-testing-8.jpgAnti-RPL32 Antibody Picoband® IF analysis of RPL32 using anti-RPL32 antibody (A06487-2). RPL32 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RPL32 Antibody (A06487-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06487-2-rpl32-primary-antibodies-fcm-testing-9.jpgAnti-RPL32 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-RPL32 antibody (A06487-2). Overlay histogram showing K562 cells stained with A06487-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL32 Antibody (A06487-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sept5-septin5-picoband-trade-antibody-a11138-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11138-septin5-primary-antibodies-wb-testing-1.jpgAnti-SEPT5/SEPTIN5 Antibody Picoband® Western blot analysis of SEPT5/SEPTIN5 using anti-SEPT5/SEPTIN5 antibody (A11138). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT5/SEPTIN5 antigen affinity purified polyclonal antibody (Catalog # A11138) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT5/SEPTIN5 at approximately 42 kDa. The expected band size for SEPT5/SEPTIN5 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11138-septin5-primary-antibodies-fcm-testing-2.jpgAnti-SEPT5/SEPTIN5 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SEPT5/SEPTIN5 antibody (A11138). Overlay histogram showing K562 cells stained with A11138 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT5/SEPTIN5 Antibody (A11138, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11138-septin5-primary-antibodies-fcm-testing-3.jpgAnti-SEPT5/SEPTIN5 Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-SEPT5/SEPTIN5 antibody (A11138). Overlay histogram showing NRK cells stained with A11138 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT5/SEPTIN5 Antibody (A11138, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd98-slc3a2-picoband-trade-antibody-a01794-3-boster.html2026-03-17T05:13:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01794-3-slc3a2-primary-antibodies-wb-testing-1.jpgAnti-CD98/Slc3a2 Antibody Picoband® Western blot analysis of CD98/Slc3a2 using anti-CD98/Slc3a2 antibody (A01794-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse pancreas tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD98/Slc3a2 antigen affinity purified polyclonal antibody (Catalog # A01794-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD98/Slc3a2 at approximately 80-90 kDa. The expected band size for CD98/Slc3a2 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01794-3-slc3a2-primary-antibodies-fcm-testing-2.jpgAnti-CD98/Slc3a2 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-CD98/Slc3a2 antibody (A01794-3). Overlay histogram showing RAW264.7 cells stained with A01794-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD98/Slc3a2 Antibody (A01794-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc5a7-picoband-trade-antibody-a05277-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05277-1-slc5a7-primary-antibodies-wb-testing-1.jpgAnti-SLC5A7 Antibody Picoband® Western blot analysis of SLC5A7 using anti-SLC5A7 antibody (A05277-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: humna RT4 whle cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A7 antigen affinity purified polyclonal antibody (Catalog # A05277-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A7 at approximately 80 kDa. The expected band size for SLC5A7 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05277-1-slc5a7-primary-antibodies-fcm-testing-2.jpgAnti-SLC5A7 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-SLC5A7 antibody (A05277-1). Overlay histogram showing JK cells stained with A05277-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A7 Antibody (A05277-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmed4-picoband-trade-antibody-a12647-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12647-1-tmed4-primary-antibodies-wb-testing-1.jpgAnti-TMED4 Antibody Picoband® Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-bi-1-tmbim6-picoband-trade-antibody-a05462-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05462-tmbim6-primary-antibodies-wb-testing-1.jpgAnti-BI-1/TMBIM6 Antibody Picoband® Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05462-tmbim6-primary-antibodies-ihc-testing-2.jpgAnti-BI-1/TMBIM6 Antibody Picoband® IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). BI-1/TMBIM6 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BI-1/TMBIM6 Antibody (A05462) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05462-tmbim6-primary-antibodies-ihc-testing-3.jpgAnti-BI-1/TMBIM6 Antibody Picoband® IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). BI-1/TMBIM6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BI-1/TMBIM6 Antibody (A05462) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05462-tmbim6-primary-antibodies-ihc-testing-4.jpgAnti-BI-1/TMBIM6 Antibody Picoband® IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). BI-1/TMBIM6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BI-1/TMBIM6 Antibody (A05462) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05462-tmbim6-primary-antibodies-ihc-testing-5.jpgAnti-BI-1/TMBIM6 Antibody Picoband® IHC analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). BI-1/TMBIM6 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BI-1/TMBIM6 Antibody (A05462) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05462-tmbim6-primary-antibodies-if-testing-6.jpgAnti-BI-1/TMBIM6 Antibody Picoband® IF analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). BI-1/TMBIM6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BI-1/TMBIM6 Antibody (A05462) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05462-tmbim6-primary-antibodies-if-testing-7.jpgAnti-BI-1/TMBIM6 Antibody Picoband® IF analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). BI-1/TMBIM6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-BI-1/TMBIM6 Antibody (A05462) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1225332026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00001-2-tp53-primary-antibodies-wb-testing-1.jpgAnti-TP53 Antibody Picoband® Western blot analysis of TP53 using anti-TP53 antibody (A00001-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 antigen affinity purified polyclonal antibody (Catalog # A00001-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53 at approximately 53 kDa. The expected band size for TP53 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00001-2-tp53-primary-antibodies-ihc-testing-2.jpgAnti-TP53 Antibody Picoband® IHC analysis of TP53 using anti-TP53 antibody (A00001-2). TP53 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53 Antibody (A00001-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00001-2-tp53-primary-antibodies-ihc-testing-3.jpgAnti-TP53 Antibody Picoband® IHC analysis of TP53 using anti-TP53 antibody (A00001-2). TP53 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53 Antibody (A00001-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00001-2-tp53-primary-antibodies-fcm-testing-4.jpgAnti-TP53 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-TP53 antibody (A00001-2). Overlay histogram showing A431 cells stained with A00001-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TP53 Antibody (A00001-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-vps36-picoband-trade-antibody-a07809-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07809-1-vps36-primary-antibodies-wb-testing-1.jpgAnti-VPS36 Antibody Picoband® Western blot analysis of VPS36 using anti-VPS36 antibody (A07809-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human U937 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS36 antigen affinity purified polyclonal antibody (Catalog # A07809-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS36 at approximately 44 kDa. The expected band size for VPS36 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07809-1-vps36-primary-antibodies-fcm-testing-2.jpgAnti-VPS36 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-VPS36 antibody (A07809-1). Overlay histogram showing PC-3 cells stained with A07809-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS36 Antibody (A07809-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-vps41-picoband-trade-antibody-a04135-2-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04135-2-vps41-primary-antibodies-wb-testing-1.jpgAnti-VPS41 Antibody Picoband® Western blot analysis of VPS41 using anti-VPS41 antibody (A04135-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS41 antigen affinity purified polyclonal antibody (Catalog # A04135-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS41 at approximately 99 kDa. The expected band size for VPS41 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04135-2-vps41-primary-antibodies-fcm-testing-2.jpgAnti-VPS41 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-VPS41 antibody (A04135-2). Overlay histogram showing A549 cells stained with A04135-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS41 Antibody (A04135-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ywhae-picoband-trade-antibody-a01687-5-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01687-5-ywhae-primary-antibodies-wb-testing-1.jpgAnti-YWHAE Antibody Picoband® Western blot analysis of YWHAE using anti-YWHAE antibody (A01687-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAE antigen affinity purified polyclonal antibody (Catalog # A01687-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YWHAE at approximately 32 kDa. The expected band size for YWHAE is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01687-5-ywhae-primary-antibodies-ihc-testing-2.jpgAnti-YWHAE Antibody Picoband® IHC analysis of YWHAE using anti-YWHAE antibody (A01687-5). YWHAE was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YWHAE Antibody (A01687-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01687-5-ywhae-primary-antibodies-ihc-testing-3.jpgAnti-YWHAE Antibody Picoband® IHC analysis of YWHAE using anti-YWHAE antibody (A01687-5). YWHAE was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YWHAE Antibody (A01687-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01687-5-ywhae-primary-antibodies-ihc-testing-4.jpgAnti-YWHAE Antibody Picoband® IHC analysis of YWHAE using anti-YWHAE antibody (A01687-5). YWHAE was detected in a paraffin-embedded section of mouse brain tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YWHAE Antibody (A01687-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01687-5-ywhae-primary-antibodies-ihc-testing-5.jpgAnti-YWHAE Antibody Picoband® IHC analysis of YWHAE using anti-YWHAE antibody (A01687-5). YWHAE was detected in a paraffin-embedded section of rat brain tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YWHAE Antibody (A01687-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01687-5-ywhae-primary-antibodies-if-testing-6.jpgAnti-YWHAE Antibody Picoband® IF analysis of YWHAE using anti-YWHAE antibody (A01687-5). YWHAE was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-YWHAE Antibody (A01687-5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/picokine-elisa-kits/human-erbb4-picokine-elisa-kit-ek2195-boster.html2026-03-10T04:34:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2195.pngHuman ERBB4 ELISA Kit PicoKine®Human ERBB4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-esam-picokine-elisa-kit-ek2196-boster.html2026-03-10T04:34:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2196.jpgHuman ESAM ELISA Kit PicoKine®Human ESAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-f7-picokine-elisa-kit-ek2197-boster.html2026-03-10T04:34:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2197.jpgHuman F7 ELISA Kit PicoKine®Human F7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-f10-picokine-elisa-kit-ek2198-boster.html2026-03-10T04:34:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2198.jpgHuman F10 ELISA Kit PicoKine®Human F10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-f11-picokine-elisa-kit-ek2199-boster.html2026-03-10T04:34:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2199.jpgHuman F11 ELISA Kit PicoKine®Human F11 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-enos-nos3-picokine-elisa-kit-ek0754-boster.html2026-03-10T04:34:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0754.jpgHuman eNOS/NOS3 ELISA Kit PicoKine®Human NOS3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd89-picokine-elisa-kit-ek2200-boster.html2026-03-10T04:34:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2200.pngHuman CD89 ELISA Kit PicoKine®Human CD89 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-acaca-picoband-trade-antibody-a01802-2-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01802-2-acaca-primary-antibodies-wb-testing-1.jpgAnti-ACACA Antibody Picoband® Western blot analysis of ACACA using anti-ACACA antibody (A01802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACA antigen affinity purified polyclonal antibody (Catalog # A01802-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACA at approximately 266 kDa. The expected band size for ACACA is at 266 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-adamts9-picoband-trade-antibody-a04639-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04639-1-adamts9-primary-antibodies-wb-testing-1.jpgAnti-ADAMTS9 Antibody Picoband® Western blot analysis of ADAMTS9 using anti-ADAMTS9 antibody (A04639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat H9C2(2-1) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS9 antigen affinity purified polyclonal antibody (Catalog # A04639-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS9 at approximately 216 kDa. The expected band size for ADAMTS9 is at 216 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04639-1-adamts9-primary-antibodies-fcm-testing-2.jpgAnti-ADAMTS9 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-ADAMTS9 antibody (A04639-1). Overlay histogram showing THP-1 cells stained with A04639-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAMTS9 Antibody (A04639-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04639-1-adamts9-primary-antibodies-fcm-testing-3.jpgAnti-ADAMTS9 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-ADAMTS9 antibody (A04639-1). Overlay histogram showing U20S cells stained with A04639-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAMTS9 Antibody (A04639-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-red1-adarb1-picoband-trade-antibody-a01810-2-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01810-2-adarb1-primary-antibodies-wb-testing-1.jpgAnti-RED1/ADARB1 Antibody Picoband® Western blot analysis of RED1/ADARB1 using anti-RED1/ADARB1 antibody (A01810-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RED1/ADARB1 antigen affinity purified polyclonal antibody (Catalog # A01810-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RED1/ADARB1 at approximately 90 kDa. The expected band size for RED1/ADARB1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01810-2-adarb1-primary-antibodies-fcm-testing-2.jpgAnti-RED1/ADARB1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-RED1/ADARB1 antibody (A01810-2). Overlay histogram showing 293T cells stained with A01810-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RED1/ADARB1 Antibody (A01810-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01810-2-adarb1-primary-antibodies-fcm-testing-3.jpgAnti-RED1/ADARB1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RED1/ADARB1 antibody (A01810-2). Overlay histogram showing HEL cells stained with A01810-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RED1/ADARB1 Antibody (A01810-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aida-picoband-trade-antibody-a04989-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04989-1-aida-primary-antibodies-wb-testing-1.jpgAnti-AIDA Antibody Picoband® Western blot analysis of AIDA using anti-AIDA antibody (A04989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIDA antigen affinity purified polyclonal antibody (Catalog # A04989-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIDA at approximately 36 kDa. The expected band size for AIDA is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04989-1-aida-primary-antibodies-fcm-testing-2.jpgAnti-AIDA Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-AIDA antibody (A04989-1). Overlay histogram showing K562 cells stained with A04989-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIDA Antibody (A04989-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-arl13b-picoband-trade-antibody-a04279-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-wb-testing-1.jpgAnti-ARL13B Antibody Picoband® Western blot analysis of ARL13B using anti-ARL13B antibody (A04279-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARL13B antigen affinity purified polyclonal antibody (Catalog # A04279-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARL13B at approximately 55 kDa. The expected band size for ARL13B is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-ihc-testing-2.jpgAnti-ARL13B Antibody Picoband® IHC analysis of ARL13B using anti-ARL13B antibody (A04279-1). ARL13B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARL13B Antibody (A04279-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-ihc-testing-3.jpgAnti-ARL13B Antibody Picoband® IHC analysis of ARL13B using anti-ARL13B antibody (A04279-1). ARL13B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARL13B Antibody (A04279-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-ihc-testing-4.jpgAnti-ARL13B Antibody Picoband® IHC analysis of ARL13B using anti-ARL13B antibody (A04279-1). ARL13B was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARL13B Antibody (A04279-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-ihc-testing-5.jpgAnti-ARL13B Antibody Picoband® IHC analysis of ARL13B using anti-ARL13B antibody (A04279-1). ARL13B was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARL13B Antibody (A04279-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-ihc-testing-6.jpgAnti-ARL13B Antibody Picoband® IHC analysis of ARL13B using anti-ARL13B antibody (A04279-1). ARL13B was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARL13B Antibody (A04279-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-if-testing-7.jpgAnti-ARL13B Antibody Picoband® IF analysis of ARL13B using anti-ARL13B antibody (A04279-1). ARL13B was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ARL13B Antibody (A04279-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04279-1-arl13b-primary-antibodies-fcm-testing-8.jpgAnti-ARL13B Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-ARL13B antibody (A04279-1). Overlay histogram showing K562 cells stained with A04279-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARL13B Antibody (A04279-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aurora-a-aurka-picoband-trade-antibody-a00246-4-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-wb-testing-1.jpgAnti-Aurora A/AURKA Antibody Picoband® Western blot analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora A/AURKA antigen affinity purified polyclonal antibody (Catalog # A00246-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora A/AURKA at approximately 46 kDa. The expected band size for Aurora A/AURKA is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-ihc-testing-2.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-4). Aurora A/AURKA was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-ihc-testing-3.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-4). Aurora A/AURKA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-ihc-testing-4.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-4). Aurora A/AURKA was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-ihc-testing-5.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-4). Aurora A/AURKA was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-ihc-testing-6.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-4). Aurora A/AURKA was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-ihc-testing-7.jpgAnti-Aurora A/AURKA Antibody Picoband® IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody (A00246-4). Aurora A/AURKA was detected in a paraffin-embedded section of human renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aurora A/AURKA Antibody (A00246-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-4-aurka-primary-antibodies-fcm-testing-8.jpgAnti-Aurora A/AURKA Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Aurora A/AURKA antibody (A00246-4). Overlay histogram showing HepG2 cells stained with A00246-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aurora A/AURKA Antibody (A00246-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-aurora-a-aurka-picoband-trade-antibody-a00246-5-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00246-5-aurka-primary-antibodies-wb-testing-1.jpgAnti-Aurora A/Aurka Antibody Picoband® Western blot analysis of Aurora A/Aurka using anti-Aurora A/Aurka antibody (A00246-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aurora A/Aurka antigen affinity purified polyclonal antibody (Catalog # A00246-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aurora A/Aurka at approximately 48 kDa. The expected band size for Aurora A/Aurka is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-b3gnt2-picoband-trade-antibody-a09524-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09524-1-b3gnt2-primary-antibodies-wb-testing-1.jpgAnti-B3GNT2 Antibody Picoband® Western blot analysis of B3GNT2 using anti-B3GNT2 antibody (A09524-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B3GNT2 antigen affinity purified polyclonal antibody (Catalog # A09524-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B3GNT2 at approximately 55 kDa. The expected band size for B3GNT2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09524-1-b3gnt2-primary-antibodies-if-testing-2.jpgAnti-B3GNT2 Antibody Picoband® IF analysis of B3GNT2 using anti-B3GNT2 antibody (A09524-1). B3GNT2 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-B3GNT2 Antibody (A09524-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09524-1-b3gnt2-primary-antibodies-fcm-testing-3_1.jpgAnti-B3GNT2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-B3GNT2 antibody (A09524-1). Overlay histogram showing Hela cells stained with A09524-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B3GNT2 Antibody (A09524-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09524-1-b3gnt2-primary-antibodies-fcm-testing-4.jpgAnti-B3GNT2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-B3GNT2 antibody (A09524-1). Overlay histogram showing THP-1 cells stained with A09524-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B3GNT2 Antibody (A09524-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cad-picoband-trade-antibody-a00463-4-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-wb-testing-1.jpgAnti-CAD Antibody Picoband® Western blot analysis of CAD using anti-CAD antibody (A00463-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAD antigen affinity purified polyclonal antibody (Catalog # A00463-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAD at approximately 260 kDa. The expected band size for CAD is at 243 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-ihc-testing-2.jpgAnti-CAD Antibody Picoband® IHC analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in a paraffin-embedded section of human gastric lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-ihc-testing-3.jpgAnti-CAD Antibody Picoband® IHC analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-ihc-testing-4.jpgAnti-CAD Antibody Picoband® IHC analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-ihc-testing-5.jpgAnti-CAD Antibody Picoband® IHC analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-ihc-testing-6.jpgAnti-CAD Antibody Picoband® IHC analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in a paraffin-embedded section of human squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-ihc-testing-7.jpgAnti-CAD Antibody Picoband® IHC analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-ihc-testing-8.jpgAnti-CAD Antibody Picoband® IHC analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00463-4-cad-primary-antibodies-if-testing-9.jpgAnti-CAD Antibody Picoband® IF analysis of CAD using anti-CAD antibody (A00463-4). CAD was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CAD Antibody (A00463-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cdx2-picoband-trade-antibody-a00877-3-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00877-3-cdx2-primary-antibodies-wb-testing-1.jpgAnti-CDX2 Antibody Picoband® Western blot analysis of CDX2 using anti-CDX2 antibody (A00877-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human DLD1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDX2 antigen affinity purified polyclonal antibody (Catalog # A00877-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDX2 at approximately 45 kDa. The expected band size for CDX2 is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cenpf-picoband-trade-antibody-a03311-1-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03311-1-cenpf-primary-antibodies-wb-testing-1.jpgAnti-CENPF Antibody Picoband® Western blot analysis of CENPF using anti-CENPF antibody (A03311-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPF antigen affinity purified polyclonal antibody (Catalog # A03311-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPF at approximately 358 kDa. The expected band size for CENPF is at 358 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03311-1-cenpf-primary-antibodies-if-testing-2.jpgAnti-CENPF Antibody Picoband® IF analysis of CENPF using anti-CENPF antibody (A03311-1). CENPF was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CENPF Antibody (A03311-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03311-1-cenpf-primary-antibodies-fcm-testing-3.jpgAnti-CENPF Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CENPF antibody (A03311-1). Overlay histogram showing HepG2 cells stained with A03311-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CENPF Antibody (A03311-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ykl-40-chi3l1-picoband-trade-antibody-a01283-3-boster.html2026-03-17T05:13:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01283-3-chi3l1-primary-antibodies-wb-testing-1.jpgAnti-YKL-40/CHI3L1 Antibody Picoband® Western blot analysis of YKL-40/CHI3L1 using anti-YKL-40/CHI3L1 antibody (A01283-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YKL-40/CHI3L1 antigen affinity purified polyclonal antibody (Catalog # A01283-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YKL-40/CHI3L1 at approximately 43 kDa. The expected band size for YKL-40/CHI3L1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01283-3-chi3l1-primary-antibodies-if-testing-2.jpgAnti-YKL-40/CHI3L1 Antibody Picoband® IF analysis of YKL-40/CHI3L1 using anti-YKL-40/CHI3L1 antibody (A01283-3). YKL-40/CHI3L1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-YKL-40/CHI3L1 Antibody (A01283-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-dhx29-picoband-trade-antibody-a07866-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07866-dhx29-primary-antibodies-wb-testing-1.jpgAnti-DHX29 Antibody Picoband® Western blot analysis of DHX29 using anti-DHX29 antibody (A07866). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HT1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX29 antigen affinity purified polyclonal antibody (Catalog # A07866) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHX29 at approximately 155 kDa. The expected band size for DHX29 is at 155 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07866-dhx29-primary-antibodies-fcm-testing-2.jpgAnti-DHX29 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-DHX29 antibody (A07866). Overlay histogram showing 293T cells stained with A07866 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHX29 Antibody (A07866, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dnajc3-picoband-trade-antibody-a04805-1-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04805-1-dnajc3-primary-antibodies-wb-testing-1.jpgAnti-DNAJC3 Antibody Picoband® Western blot analysis of DNAJC3 using anti-DNAJC3 antibody (A04805-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse pancreas tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC3 antigen affinity purified polyclonal antibody (Catalog # A04805-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNAJC3 at approximately 58 kDa. The expected band size for DNAJC3 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-dnmt1-picoband-trade-antibody-a00172-3-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00172-3-dnmt1-primary-antibodies-wb-testing-1.jpgAnti-Dnmt1 Antibody Picoband® Western blot analysis of Dnmt1 using anti-Dnmt1 antibody (A00172-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt1 antigen affinity purified polyclonal antibody (Catalog # A00172-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dnmt1 at approximately 200 kDa. The expected band size for Dnmt1 is at 183 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-dock4-picoband-trade-antibody-a05916-1-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05916-1-dock4-primary-antibodies-wb-testing-1.jpgAnti-DOCK4 Antibody Picoband® Western blot analysis of DOCK4 using anti-DOCK4 antibody (A05916-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat L6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK4 antigen affinity purified polyclonal antibody (Catalog # A05916-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DOCK4 at approximately 250 kDa. The expected band size for DOCK4 is at 225 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05916-1-dock4-primary-antibodies-ihc-testing-2.jpgAnti-DOCK4 Antibody Picoband® IHC analysis of DOCK4 using anti-DOCK4 antibody (A05916-1). DOCK4 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOCK4 Antibody (A05916-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05916-1-dock4-primary-antibodies-fcm-testing-3.jpgAnti-DOCK4 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-DOCK4 antibody (A05916-1). Overlay histogram showing SiHa cells stained with A05916-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DOCK4 Antibody (A05916-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-p97-dap5-eif4g2-picoband-trade-antibody-a03888-1-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03888-1-eif4g2-primary-antibodies-ip-testing-1.jpgAnti-P97/DAP5/EIF4G2 Antibody Picoband®Immunoprecipitating (IP) P97/DAP5/EIF4G2 in Hela whole cell lysate. Western blot analysis of P97/DAP5/EIF4G2 using anti-P97/DAP5/EIF4G2 antibody (A03888-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-P97/DAP5/EIF4G2 antibody in Hela whole cell lysate; Lane 3: anti-P97/DAP5/EIF4G2 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P97/DAP5/EIF4G2 antigen affinity purified polyclonal antibody (A03888-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for P97/DAP5/EIF4G2 at approximately 97 kDa. The expected band size for P97/DAP5/EIF4G2 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03888-1-eif4g2-primary-antibodies-wb-testing-1_1.jpgAnti-P97/DAP5/EIF4G2 Antibody Picoband® Western blot analysis of P97/DAP5/EIF4G2 using anti-P97/DAP5/EIF4G2 antibody (A03888-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5:mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P97/DAP5/EIF4G2 antigen affinity purified polyclonal antibody (Catalog # A03888-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P97/DAP5/EIF4G2 at approximately 97 kDa. The expected band size for P97/DAP5/EIF4G2 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03888-1-eif4g2-primary-antibodies-fcm-testing-3.jpgAnti-P97/DAP5/EIF4G2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-P97/DAP5/EIF4G2 antibody (A03888-1). Overlay histogram showing THP-1 cells stained with A03888-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P97/DAP5/EIF4G2 Antibody (A03888-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03888-1-eif4g2-primary-antibodies-if-testing-2.jpgAnti-P97/DAP5/EIF4G2 Antibody Picoband® IF analysis of P97/DAP5/EIF4G2 using anti-P97/DAP5/EIF4G2 antibody (A03888-1). P97/DAP5/EIF4G2 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P97/DAP5/EIF4G2 Antibody (A03888-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ergic1-picoband-trade-antibody-a11007-1-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11007-1-ergic1-primary-antibodies-wb-testing-1.jpgAnti-ERGIC1 Antibody Picoband® Western blot analysis of ERGIC1 using anti-ERGIC1 antibody (A11007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERGIC1 antigen affinity purified polyclonal antibody (Catalog # A11007-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERGIC1 at approximately 35 kDa. The expected band size for ERGIC1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11007-1-ergic1-primary-antibodies-if-testing-4.jpgAnti-ERGIC1 Antibody Picoband® IF analysis of ERGIC1 using anti-ERGIC1 antibody (A11007-1). ERGIC1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ERGIC1 Antibody (A11007-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11007-1-ergic1-primary-antibodies-ihc-testing-1.jpgAnti-ERGIC1 Antibody Picoband®IHC analysis of ERGIC1 using anti-ERGIC1 antibody (A11007-1). ERGIC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERGIC1 Antibody (A11007-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11007-1-ergic1-primary-antibodies-ihc-testing-2.jpgAnti-ERGIC1 Antibody Picoband®IHC analysis of ERGIC1 using anti-ERGIC1 antibody (A11007-1). ERGIC1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERGIC1 Antibody (A11007-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11007-1-ergic1-primary-antibodies-ip-testing-1.jpgAnti-ERGIC1 Antibody Picoband®Immunoprecipitating (IP) ERGIC1 in SH-SY5Y whole cell lysate. Western blot analysis of ERGIC1 using anti-ERGIC1 antibody (A11007-1); Lane 1: SH-SY5Y whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-ERGIC1 antibody in SH-SY5Y whole cell lysate; Lane 3: anti-ERGIC1 antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERGIC1 antigen affinity purified polyclonal antibody (A11007-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for ERGIC1 at approximately 35 kDa. The expected band size for ERGIC1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11007-1-ergic1-primary-antibodies-fcm-testing-2.jpgAnti-ERGIC1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-ERGIC1 antibody (A11007-1). Overlay histogram showing MCF-7 cells stained with A11007-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERGIC1 Antibody (A11007-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11007-1-ergic1-primary-antibodies-fcm-testing-3.jpgAnti-ERGIC1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-ERGIC1 antibody (A11007-1). Overlay histogram showing U87 cells stained with A11007-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERGIC1 Antibody (A11007-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-exonuclease-1-exo1-picoband-trade-antibody-a00536-3-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00536-3-exo1-primary-antibodies-wb-testing-1_1.jpgAnti-Exonuclease 1/EXO1 Antibody Picoband®Western blot analysis of EXO1 using anti-EXO1 antibody (A00536-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXO1 antigen affinity purified polyclonal antibody (A00536-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXO1 at approximately 120 kDa. The expected band size for EXO1 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00536-3-exo1-primary-antibodies-if-testing-1.jpgAnti-Exonuclease 1/EXO1 Antibody Picoband®IF analysis of EXO1 using anti-EXO1 antibody (A00536-3) and anti-Tubulin Alpha antibody (M03989-3). EXO1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EXO1 Antibody (A00536-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00536-3-exo1-primary-antibodies-ip-testing-1.jpgAnti-Exonuclease 1/EXO1 Antibody Picoband®Immunoprecipitating (IP) EXO1 in SH-SY5Y whole cell lysate. Western blot analysis of EXO1 using anti-EXO1 antibody (A00536-3); Lane 1: SH-SY5Y whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-EXO1 antibody in SH-SY5Y whole cell lysate; Lane 3: anti-EXO1 antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EXO1 antigen affinity purified polyclonal antibody (A00536-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for EXO1 at approximately 120 kDa. The expected band size for EXO1 is at 94 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-fibroblast-activation-protein-alpha-fap-picoband-trade-antibody-a00422-5-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00422-5-fap-primary-antibodies-wb-testing-1.jpgAnti-Fibroblast activation protein, alpha/FAP Antibody Picoband® Western blot analysis of Fibroblast activation protein, alpha/FAP using anti-Fibroblast activation protein, alpha/FAP antibody (A00422-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibroblast activation protein, alpha/FAP antigen affinity purified polyclonal antibody (Catalog # A00422-5) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibroblast activation protein, alpha/FAP at approximately 97 kDa. The expected band size for Fibroblast activation protein, alpha/FAP is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00422-5-fap-primary-antibodies-fcm-testing-2.jpgAnti-Fibroblast activation protein, alpha/FAP Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Fibroblast activation protein, alpha/FAP antibody (A00422-5). Overlay histogram showing SiHa cells stained with A00422-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibroblast activation protein, alpha/FAP Antibody (A00422-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gsk3-beta-gsk3b-picoband-trade-antibody-a00791-3-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-wb-testing-1_1.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® Western blot analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 beta/GSK3B antigen affinity purified polyclonal antibody (Catalog # A00791-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 beta/GSK3B at approximately 46 kDa. The expected band size for GSK3 beta/GSK3B is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-2_1.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). GSK3 beta/GSK3B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-8.jpgAnti-GSK3 beta/GSK3B Antibody Picoband®IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-3_1.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). GSK3 beta/GSK3B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-4.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). GSK3 beta/GSK3B was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-5.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). GSK3 beta/GSK3B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-6.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). GSK3 beta/GSK3B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-7.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® IHC analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). GSK3 beta/GSK3B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-7.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-GSK3B Antibody (A00791-3) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-8.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-GSK3B Antibody (A00791-3) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-9.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-GSK3B Antibody (A00791-3) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-10.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-GSK3B Antibody (A00791-3) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-11.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-GSK3B Antibody (A00791-3) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-ihc-testing-12.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IHC analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-GSK3B Antibody (A00791-3) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-if-testing-8.jpgAnti-GSK3 beta/GSK3B Antibody Picoband® IF analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3) and anti-Beta Tubulin antibody (M01857-3). GSK3 beta/GSK3B was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GSK3 beta/GSK3B Antibody (A00791-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-if-testing-2_1.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IF analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-if-testing-3_1.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IF analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-if-testing-4_1.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IF analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-if-testing-5_1.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IF analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-if-testing-6_1.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IF analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00791-3-gsk3b-primary-antibodies-if-testing-7_1.pngAnti-GSK3 beta/GSK3B Antibody Picoband®IF analysis of GSK3B using anti-GSK3B antibody (A00791-3). GSK3B was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-GSK3B Antibody (A00791-3) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-granzyme-k-gzmk-picoband-trade-antibody-a06281-2-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06281-2-gzmk-primary-antibodies-ihc-testing-1.jpgAnti-Granzyme K/GZMK Antibody IHC analysis of Granzyme K/GZMK using anti-Granzyme K/GZMK antibody (A06281-2). Granzyme K/GZMK was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Granzyme K/GZMK Antibody (A06281-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-dtr-hbegf-picoband-trade-antibody-a01759-3-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01759-3-hbegf-primary-antibodies-wb-testing-1.jpgAnti-DTR/HBEGF Antibody Picoband® Western blot analysis of DTR/HBEGF using anti-DTR/HBEGF antibody (A01759-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTR/HBEGF antigen affinity purified polyclonal antibody (Catalog # A01759-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DTR/HBEGF at approximately 23 kDa. The expected band size for DTR/HBEGF is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01759-3-hbegf-primary-antibodies-ihc-testing-2.jpgAnti-DTR/HBEGF Antibody Picoband® IHC analysis of DTR/HBEGF using anti-DTR/HBEGF antibody (A01759-3). DTR/HBEGF was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DTR/HBEGF Antibody (A01759-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01759-3-hbegf-primary-antibodies-ihc-testing-3.jpgAnti-DTR/HBEGF Antibody Picoband® IHC analysis of DTR/HBEGF using anti-DTR/HBEGF antibody (A01759-3). DTR/HBEGF was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DTR/HBEGF Antibody (A01759-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01759-3-hbegf-primary-antibodies-ihc-testing-4.jpgAnti-DTR/HBEGF Antibody Picoband® IHC analysis of DTR/HBEGF using anti-DTR/HBEGF antibody (A01759-3). DTR/HBEGF was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DTR/HBEGF Antibody (A01759-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01759-3-hbegf-primary-antibodies-ihc-testing-5.jpgAnti-DTR/HBEGF Antibody Picoband® IHC analysis of DTR/HBEGF using anti-DTR/HBEGF antibody (A01759-3). DTR/HBEGF was detected in a paraffin-embedded section of rat respiratory smooth muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DTR/HBEGF Antibody (A01759-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01759-3-hbegf-primary-antibodies-if-testing-6.jpgAnti-DTR/HBEGF Antibody Picoband® IF analysis of DTR/HBEGF using anti-DTR/HBEGF antibody (A01759-3). DTR/HBEGF was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DTR/HBEGF Antibody (A01759-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01759-3-hbegf-primary-antibodies-fcm-testing-7.jpgAnti-DTR/HBEGF Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-DTR/HBEGF antibody (A01759-3). Overlay histogram showing Jurkat cells stained with A01759-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DTR/HBEGF Antibody (A01759-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hmgcr-picoband-trade-antibody-a00643-3-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00643-3-hmgcr-primary-antibodies-wb-testing-1.jpgAnti-HMGCR Antibody Picoband® Western blot analysis of HMGCR using anti-HMGCR antibody (A00643-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGCR antigen affinity purified polyclonal antibody (Catalog # A00643-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGCR at approximately 97 kDa. The expected band size for HMGCR is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00643-3-hmgcr-primary-antibodies-ihc-testing-2.jpgAnti-HMGCR Antibody Picoband® IHC analysis of HMGCR using anti-HMGCR antibody (A00643-3). HMGCR was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGCR Antibody (A00643-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00643-3-hmgcr-primary-antibodies-ihc-testing-3.jpgAnti-HMGCR Antibody Picoband® IHC analysis of HMGCR using anti-HMGCR antibody (A00643-3). HMGCR was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGCR Antibody (A00643-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00643-3-hmgcr-primary-antibodies-ihc-testing-4.jpgAnti-HMGCR Antibody Picoband® IHC analysis of HMGCR using anti-HMGCR antibody (A00643-3). HMGCR was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGCR Antibody (A00643-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00643-3-hmgcr-primary-antibodies-if-testing-5.jpgAnti-HMGCR Antibody Picoband® IF analysis of HMGCR using anti-HMGCR antibody (A00643-3). HMGCR was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HMGCR Antibody (A00643-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00643-3-hmgcr-primary-antibodies-fcm-testing-6.jpgAnti-HMGCR Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-HMGCR antibody (A00643-3). Overlay histogram showing HEL cells stained with A00643-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGCR Antibody (A00643-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-heme-oxygenase-2-hmox2-picoband-trade-antibody-a05506-2-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05506-2-hmox2-primary-antibodies-wb-testing-1.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband® Western blot analysis of Heme oxygenase 2/HMOX2 using anti-Heme oxygenase 2/HMOX2 antibody (A05506-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates. Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme oxygenase 2/HMOX2 antigen affinity purified polyclonal antibody (Catalog # A05506-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme oxygenase 2/HMOX2 at approximately 38 kDa. The expected band size for Heme oxygenase 2/HMOX2 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05506-2-hmox2-primary-antibodies-ihc-testing-2.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband® IHC analysis of Heme oxygenase 2/HMOX2 using anti-Heme oxygenase 2/HMOX2 antibody (A05506-2). Heme oxygenase 2/HMOX2 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Heme oxygenase 2/HMOX2 Antibody (A05506-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05506-2-hmox2-primary-antibodies-ihc-testing-3.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband® IHC analysis of Heme oxygenase 2/HMOX2 using anti-Heme oxygenase 2/HMOX2 antibody (A05506-2). Heme oxygenase 2/HMOX2 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Heme oxygenase 2/HMOX2 Antibody (A05506-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05506-2-hmox2-primary-antibodies-ihc-testing-4.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband® IHC analysis of Heme oxygenase 2/HMOX2 using anti-Heme oxygenase 2/HMOX2 antibody (A05506-2). Heme oxygenase 2/HMOX2 was detected in a paraffin-embedded section of human ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Heme oxygenase 2/HMOX2 Antibody (A05506-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05506-2-hmox2-primary-antibodies-if-testing-5.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband® IF analysis of Heme oxygenase 2/HMOX2 using anti-Heme oxygenase 2/HMOX2 antibody (A05506-2). Heme oxygenase 2/HMOX2 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Heme oxygenase 2/HMOX2 Antibody (A05506-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05506-2-hmox2-primary-antibodies-fcm-testing-6.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Heme oxygenase 2/HMOX2 antibody (A05506-2). Overlay histogram showing MCF-7 cells stained with A05506-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Heme oxygenase 2/HMOX2 Antibody (A05506-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hnrnp-k-hnrnpk-picoband-trade-antibody-a01793-2-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01793-2-hnrnpk-primary-antibodies-wb-testing-1.jpgAnti-hnRNP K/HNRNPK Antibody Picoband® Western blot analysis of hnRNP K/HNRNPK using anti-hnRNP K/HNRNPK antibody (A01793-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP K/HNRNPK antigen affinity purified polyclonal antibody (Catalog # A01793-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP K/HNRNPK at approximately 60 kDa. The expected band size for hnRNP K/HNRNPK is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01793-2-hnrnpk-primary-antibodies-if-testing-2.jpgAnti-hnRNP K/HNRNPK Antibody Picoband® IF analysis of hnRNP K/HNRNPK using anti-hnRNP K/HNRNPK antibody (A01793-2). hnRNP K/HNRNPK was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-hnRNP K/HNRNPK Antibody (A01793-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01793-2-hnrnpk-primary-antibodies-fcm-testing-3.jpgAnti-hnRNP K/HNRNPK Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-hnRNP K/HNRNPK antibody (A01793-2). Overlay histogram showing HepG2 cells stained with A01793-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP K/HNRNPK Antibody (A01793-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01793-2-hnrnpk-primary-antibodies-fcm-testing-4.jpgAnti-hnRNP K/HNRNPK Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-hnRNP K/HNRNPK antibody (A01793-2). Overlay histogram showing ANA-1 cells stained with A01793-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP K/HNRNPK Antibody (A01793-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01793-2-hnrnpk-primary-antibodies-fcm-testing-5.jpgAnti-hnRNP K/HNRNPK Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-hnRNP K/HNRNPK antibody (A01793-2). Overlay histogram showing C6 cells stained with A01793-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP K/HNRNPK Antibody (A01793-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hnrnp-l-hnrnpl-picoband-trade-antibody-a03769-4-boster.html2026-03-17T05:13:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-wb-testing-1_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® Western blot analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP L/HNRNPL antigen affinity purified polyclonal antibody (Catalog # A03769-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP L/HNRNPL at approximately 68 kDa. The expected band size for hnRNP L/HNRNPL is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-2_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-3_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-4_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-5_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-6_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-7_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-8_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-9_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-10_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-11_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-ihc-testing-12_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IHC analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-if-testing-13_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IF analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-if-testing-14.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IF analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03769-4-hnrnpl-primary-antibodies-fcm-testing-15_1.jpgAnti-hnRNP L/HNRNPL Antibody Picoband® IF analysis of hnRNP L/HNRNPL using anti-hnRNP L/HNRNPL antibody (A03769-4). hnRNP L/HNRNPL was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP L/HNRNPL Antibody (A03769-4) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-hnrnp-u-p120-hnrnpu-picoband-trade-antibody-a03691-3-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-wb-testing-1.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® Western blot analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP U/p120/HNRNPU antigen affinity purified polyclonal antibody (Catalog # A03691-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP U/p120/HNRNPU at approximately 120 kDa. The expected band size for hnRNP U/p120/HNRNPU is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-2.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-3.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-4.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-5.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-6.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-7.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-8.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-9.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-ihc-testing-10.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IHC analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-if-testing-11.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-fcm-testing-12_1.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). Overlay histogram showing HEL cells stained with A03691-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-fcm-testing-13_1.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). Overlay histogram showing C6 cells stained with A03691-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-if-testing-14.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-if-testing-15.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03691-3-hnrnpu-primary-antibodies-if-testing-16.jpgAnti-hnRNP U/p120/HNRNPU Antibody Picoband® IF analysis of hnRNP U/p120/HNRNPU using anti-hnRNP U/p120/HNRNPU antibody (A03691-3). hnRNP U/p120/HNRNPU was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-hnRNP U/p120/HNRNPU Antibody (A03691-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-5-ht2c-receptor-htr2c-picoband-trade-antibody-a01593-1-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01593-1-htr2c-primary-antibodies-wb-testing-1.jpgAnti-5-HT2C Receptor/HTR2C Antibody Picoband® Western blot analysis of 5-HT2C Receptor/HTR2C using anti-5-HT2C Receptor/HTR2C antibody (A01593-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5-HT2C Receptor/HTR2C antigen affinity purified polyclonal antibody (Catalog # A01593-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5-HT2C Receptor/HTR2C at approximately 75 kDa. The expected band size for 5-HT2C Receptor/HTR2C is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-st2-il1rl1-picoband-trade-antibody-a00492-2-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00492-2-il1rl1-primary-antibodies-wb-testing-1.jpgAnti-ST2/IL1RL1 Antibody Picoband® Western blot analysis of ST2/IL1RL1 using anti-ST2/IL1RL1 antibody (A00492-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST2/IL1RL1 antigen affinity purified polyclonal antibody (Catalog # A00492-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST2/IL1RL1 at approximately 70 kDa. The expected band size for ST2/IL1RL1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00492-2-il1rl1-primary-antibodies-fcm-testing-2.jpgAnti-ST2/IL1RL1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-ST2/IL1RL1 antibody (A00492-2). Overlay histogram showing HEL cells stained with A00492-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ST2/IL1RL1 Antibody (A00492-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-irf7-picoband-trade-antibody-a00115-3-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00115-3-irf7-primary-antibodies-wb-testing-1.jpgAnti-IRF7 Antibody Picoband® Western blot analysis of IRF7 using anti-IRF7 antibody (A00115-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF7 antigen affinity purified polyclonal antibody (Catalog # A00115-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF7 at approximately 70 kDa. The expected band size for IRF7 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00115-3-irf7-primary-antibodies-ihc-testing-2.jpgAnti-IRF7 Antibody Picoband® IHC analysis of IRF7 using anti-IRF7 antibody (A00115-3). IRF7 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRF7 Antibody (A00115-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00115-3-irf7-primary-antibodies-fcm-testing-3.jpgAnti-IRF7 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-IRF7 antibody (A00115-3). Overlay histogram showing JK cells stained with A00115-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF7 Antibody (A00115-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-kcnh1-picoband-trade-antibody-a01036-4-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01036-4-kcnh1-primary-antibodies-wb-testing-1.jpgAnti-KCNH1 Antibody Picoband® Western blot analysis of KCNH1 using anti-KCNH1 antibody (A01036-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNH1 antigen affinity purified polyclonal antibody (Catalog # A01036-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNH1 at approximately 111 kDa. The expected band size for KCNH1 is at 111 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-kif1b-picoband-trade-antibody-a02951-1-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02951-1-kif1b-primary-antibodies-wb-testing-1.jpgAnti-KIF1B Antibody Picoband® Western blot analysis of KIF1B using anti-KIF1B antibody (A02951-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF1B antigen affinity purified polyclonal antibody (Catalog # A02951-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF1B at approximately 204 kDa or 199 kDa. The expected band size for KIF1B is at 204 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-klf10-picoband-trade-antibody-a03419-2-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03419-2-klf10-primary-antibodies-wb-testing-1.jpgAnti-KLF10 Antibody Picoband® Western blot analysis of KLF10 using anti-KLF10 antibody (A03419-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF10 antigen affinity purified polyclonal antibody (Catalog # A03419-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF10 at approximately 55 kDa. The expected band size for KLF10 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03419-2-klf10-primary-antibodies-if-testing-2.jpgAnti-KLF10 Antibody Picoband® IF analysis of KLF10 using anti-KLF10 antibody (A03419-2). KLF10 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-KLF10 Antibody (A03419-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-mat2a-picoband-trade-antibody-a04557-1-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04557-1-mat2a-primary-antibodies-wb-testing-1.jpgAnti-MAT2A Antibody Picoband® Western blot analysis of MAT2A using anti-MAT2A antibody (A04557-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2A antigen affinity purified polyclonal antibody (Catalog # A04557-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT2A at approximately 50 kDa. The expected band size for MAT2A is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04557-1-mat2a-primary-antibodies-ihc-testing-2.jpgAnti-MAT2A Antibody Picoband® IHC analysis of MAT2A using anti-MAT2A antibody (A04557-1). MAT2A was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAT2A Antibody (A04557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04557-1-mat2a-primary-antibodies-ihc-testing-3.jpgAnti-MAT2A Antibody Picoband® IHC analysis of MAT2A using anti-MAT2A antibody (A04557-1). MAT2A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAT2A Antibody (A04557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04557-1-mat2a-primary-antibodies-ihc-testing-4.jpgAnti-MAT2A Antibody Picoband® IHC analysis of MAT2A using anti-MAT2A antibody (A04557-1). MAT2A was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAT2A Antibody (A04557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04557-1-mat2a-primary-antibodies-ihc-testing-5.jpgAnti-MAT2A Antibody Picoband® IHC analysis of MAT2A using anti-MAT2A antibody (A04557-1). MAT2A was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAT2A Antibody (A04557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04557-1-mat2a-primary-antibodies-fcm-testing-6.jpgAnti-MAT2A Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-MAT2A antibody (A04557-1). Overlay histogram showing MCF-7 cells stained with A04557-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAT2A Antibody (A04557-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hox8-msx2-picoband-trade-antibody-a01783-2-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01783-2-msx2-primary-antibodies-wb-testing-1.jpgAnti-Hox8/MSX2 Antibody Picoband® Western blot analysis of Hox8/MSX2 using anti-Hox8/MSX2 antibody (A01783-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hox8/MSX2 antigen affinity purified polyclonal antibody (Catalog # A01783-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hox8/MSX2 at approximately 37 kDa. The expected band size for Hox8/MSX2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01783-2-msx2-primary-antibodies-fcm-testing-2.jpgAnti-Hox8/MSX2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-Hox8/MSX2 antibody (A01783-2). Overlay histogram showing PC-3 cells stained with A01783-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hox8/MSX2 Antibody (A01783-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01783-2-msx2-primary-antibodies-fcm-testing-3.jpgAnti-Hox8/MSX2 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-Hox8/MSX2 antibody (A01783-2). Overlay histogram showing RT4 cells stained with A01783-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hox8/MSX2 Antibody (A01783-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-heavy-chain-myosin-myh3-picoband-trade-antibody-a02697-2-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02697-2-myh3-primary-antibodies-wb-testing-1.jpgAnti-heavy chain Myosin/MYH3 Antibody Picoband® Western blot analysis of heavy chain Myosin/MYH3 using anti-heavy chain Myosin/MYH3 antibody (A02697-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-heavy chain Myosin/MYH3 antigen affinity purified polyclonal antibody (Catalog # A02697-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for heavy chain Myosin/MYH3 at approximately 230 kDa. The expected band size for heavy chain Myosin/MYH3 is at 224 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02697-2-myh3-primary-antibodies-fcm-testing-2_1.jpgAnti-heavy chain Myosin/MYH3 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-heavy chain Myosin/MYH3 antibody (A02697-2). Overlay histogram showing 293T cells stained with A02697-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-heavy chain Myosin/MYH3 Antibody (A02697-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nkx2-5-nkx2-5-picoband-trade-antibody-a00738-2-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00738-2-nkx2-5-primary-antibodies-wb-testing-1.jpgAnti-Nkx2.5/NKX2-5 Antibody Picoband® Western blot analysis of Nkx2.5/NKX2-5 using anti-Nkx2.5/NKX2-5 antibody (A00738-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nkx2.5/NKX2-5 antigen affinity purified polyclonal antibody (Catalog # A00738-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nkx2.5/NKX2-5 at approximately 35 kDa. The expected band size for Nkx2.5/NKX2-5 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00738-2-nkx2-5-primary-antibodies-ihc-testing-1.jpgAnti-Nkx2.5/NKX2-5 Antibody Picoband®IHC analysis of NKX2-5 using anti-NKX2-5 antibody (A00738-2). NKX2-5 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2-5 Antibody (A00738-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00738-2-nkx2-5-primary-antibodies-if-testing-2.jpgAnti-Nkx2.5/NKX2-5 Antibody Picoband® IF analysis of Nkx2.5/NKX2-5 using anti-Nkx2.5/NKX2-5 antibody (A00738-2). Nkx2.5/NKX2-5 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Nkx2.5/NKX2-5 Antibody (A00738-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00738-2-nkx2-5-primary-antibodies-fcm-testing-3.jpgAnti-Nkx2.5/NKX2-5 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-Nkx2.5/NKX2-5 antibody (A00738-2). Overlay histogram showing 293T cells stained with A00738-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nkx2.5/NKX2-5 Antibody (A00738-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-niemann-pick-c1-npc1-picoband-trade-antibody-a00428-3-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00428-3-npc1-primary-antibodies-wb-testing-1.jpgAnti-Niemann Pick C1/NPC1 Antibody Picoband® Western blot analysis of Niemann Pick C1/NPC1 using anti-Niemann Pick C1/NPC1 antibody (A00428-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C1/NPC1 antigen affinity purified polyclonal antibody (Catalog # A00428-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Niemann Pick C1/NPC1 at approximately 180 kDa. The expected band size for Niemann Pick C1/NPC1 is at 142 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00428-3-npc1-primary-antibodies-fcm-testing-2.jpgAnti-Niemann Pick C1/NPC1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Niemann Pick C1/NPC1 antibody (A00428-3). Overlay histogram showing HepG2 cells stained with A00428-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Niemann Pick C1/NPC1 Antibody (A00428-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pannexin-1-panx1-picoband-trade-antibody-a00915-2-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00915-2-panx1-primary-antibodies-wb-testing-1.jpgAnti-Pannexin 1/PANX1 Antibody Picoband® Western blot analysis of Pannexin 1/PANX1 using anti-Pannexin 1/PANX1 antibody (A00915-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pannexin 1/PANX1 antigen affinity purified polyclonal antibody (Catalog # A00915-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pannexin 1/PANX1 at approximately 48 kDa. The expected band size for Pannexin 1/PANX1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00915-2-panx1-primary-antibodies-if-testing-2.jpgAnti-Pannexin 1/PANX1 Antibody Picoband® IF analysis of Pannexin 1/PANX1 using anti-Pannexin 1/PANX1 antibody (A00915-2). Pannexin 1/PANX1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Pannexin 1/PANX1 Antibody (A00915-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00915-2-panx1-primary-antibodies-fcm-testing-3.jpgAnti-Pannexin 1/PANX1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Pannexin 1/PANX1 antibody (A00915-2). Overlay histogram showing MCF-7 cells stained with A00915-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Pannexin 1/PANX1 Antibody (A00915-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00915-2-panx1-primary-antibodies-fcm-testing-4.jpgAnti-Pannexin 1/PANX1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Pannexin 1/PANX1 antibody (A00915-2). Overlay histogram showing SiHa cells stained with A00915-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Pannexin 1/PANX1 Antibody (A00915-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pdcd4-picoband-trade-antibody-a01105-3-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01105-3-pdcd4-primary-antibodies-wb-testing-1.jpgAnti-PDCD4 Antibody Picoband® Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01105-3-pdcd4-primary-antibodies-fcm-testing-2.jpgAnti-PDCD4 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PDCD4 antibody (A01105-3). Overlay histogram showing HepG2 cells stained with A01105-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDCD4 Antibody (A01105-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ppar-alpha-ppara-picoband-trade-antibody-a00600-2-boster.html2026-03-17T05:13:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-2-ppara-primary-antibodies-wb-testing-1.jpgAnti-PPAR alpha/PPARA Antibody Picoband® Western blot analysis of PPAR alpha/PPARA using anti-PPAR alpha/PPARA antibody (A00600-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR alpha/PPARA antigen affinity purified polyclonal antibody (Catalog # A00600-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAR alpha/PPARA at approximately 52 kDa. The expected band size for PPAR alpha/PPARA is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-2-fphar-15-1423634-g005.jpgAnti-PPAR alpha/PPARA Antibody Picoband®Puerarin regulates hepatic fatty acid metabolism. (A–I) qRT-PCR detected the expressions of Cd36 , Fabp1 , Fabp4 , Acc1 , Fasn , Scd1 , Ppar-α , Cpt1α , and Pgc1α in each group of livers. (J–K) The expression of PPARα in liver was detected by Western blotting and q-PCR. (L–N) The expression of PPARα in AML-12 cells treated with puerarin (0, 20, and 40 μM) and PA (500 μM) was detected by Western blotting and q-PCR. * means p < 0.05, ** means p < 0.01, ***means p < 0.001. NFD, normal fat diet; HFD, high-fat diet; HFD200, high-fat diet with puerarin (200 mg/kg/day) intervention; HFD300, high-fat diet with puerarin (300 mg/kg/day) intervention. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1423634/full'>39055493</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-2-fphar-15-1423634-g006.jpgAnti-PPAR alpha/PPARA Antibody Picoband®FMO5/PPAR-α signaling pathway is involved in puerarin-regulated lipid metabolism. (A) Molecular interaction of puerarin in the catalytic site of FMO3/FMO5; (B) qRT-PCR detected the expressions of Fmo3/Fmo5 in each group of livers; (C–E) The expression of FMO5 in AML-12 cells which were treated by puerarin (0, 20, and 40 μM) and PA (500 μM) was detected by Western blotting and q-PCR. (F) qRT-PCR and Western blotting detected the expressions of FMO5 in liver. (G–H) FMO5 protein expression in liver was detected by Western blotting and quantification with ImageJ. * means p < 0.05, ** means p < 0.01, ***means p < 0.001. NFD, normal fat diet; HFD, high-fat diet; HFD200, high-fat diet with puerarin (200 mg/kg/day) intervention; HFD300, high-fat diet with puerarin (300 mg/kg/day) intervention. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1423634/full'>39055493</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-2-ppara-primary-antibodies-fcm-testing-2.jpgAnti-PPAR alpha/PPARA Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PPAR alpha/PPARA antibody (A00600-2). Overlay histogram showing HepG2 cells stained with A00600-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAR alpha/PPARA Antibody (A00600-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-parathyroid-hormone-receptor-1-pth1r-picoband-trade-antibody-a01588-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01588-pth1r-primary-antibodies-wb-testing-1.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband® Western blot analysis of Parathyroid Hormone Receptor 1/PTH1R using anti-Parathyroid Hormone Receptor 1/PTH1R antibody (A01588). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HUH-7 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Parathyroid Hormone Receptor 1/PTH1R antigen affinity purified polyclonal antibody (Catalog # A01588) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Parathyroid Hormone Receptor 1/PTH1R at approximately 66 kDa. The expected band size for Parathyroid Hormone Receptor 1/PTH1R is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01588-jcav06p0511g001.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®TGF-β downregulates the protein level of PTHrP. (A) HCC cell lines were serum-starved for 4 h and treated with TGF-β1 (1 ng/ml) or vehicle for 48 h. Whole cell lysates were prepared and subjected to western blot analysis using the anti-PTHrP antibody. The expression level of α-tubulin was used as a loading control. (B, C) HuH-7 cells were serum-starved and treated with TGF-β1 for 48 h at different concentrations (B) or at 1 ng/ml for different time durations (C). Cytoplasmic and nuclear lysates were prepared and subjected to western blot analysis for PTHrP. The expression level of α-tubulin and lamin A was used as a loading control for cytoplasmic and nuclear lysates, respectively. (D) Hep3B cells were transfected with EGFP-tagged PTHrP expression plasmid (EGFP-PTHrP) or the control plasmid (EGFP). 72 h after transfection, the cells were serum-starved, treated with or without TGF-β1 (1 ng/ml), and subjected to western blot analysis for PTHrP. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4439935/&orig_host=www.ncbi.nlm.nih.gov'>26000041</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01588-jcav06p0511g002.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®TGF-β induces degradation of PTHrP through the ubiquitin-proteasome pathway. (A) Hep3B cells were serum-starved and treated for 48 h with different concentrations of TGF-β1 in the presence or absence of lactacystin (5 μM). Cell lysates were prepared and subjected to western blot analysis for PTHrP with Hsp90 expression as a loading control. (B) Serum-starved HuH-7 cells were treated with or without TGF-β1 (1 ng/ml) for 48 h and subjected to whole cell lysate preparation and western blot analysis. (C) Hep3B and HuH-7 cells were co-transfected with EGFP-tagged PTHrP (EGFP-PTHrP) and HA-tagged ubiquitin (HA-Ub). 72 h after transfection, the cells were serum-starved and treated with or without TGF-β1 (1 ng/ml) and subjected to whole lysate preparation and immunoprecipitation-western blot analysis. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4439935/&orig_host=www.ncbi.nlm.nih.gov'>26000041</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01588-jcav06p0511g003.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®Smurf2 is responsible for TGF-β-induced ubiquitination of PTHrP. (A) HEK-293T cells were transfected with the plasmids as indicated, serum-starved and treated for 48 h with or without TGF-β1 (1 ng/ml). Whole cell lysates were prepared and subjected to immunoprecipitation and western blot analysis. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4439935/&orig_host=www.ncbi.nlm.nih.gov'>26000041</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01588-jcav06p0511g004.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®Immunolocalization of TGF-β and PTHrP in HCC. Human HCC specimens were stained for PTHrP (A) and TGF-β (B) by immunohistochemistry using the HRP and DAB/H 2 O 2 development system. The nuclei were counterstained with hematoxylin. Shown is a representative pattern of staining of PTHrP and TGF-β in a HCC patient tissue (ID #162475). Original magnification: x100. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4439935/&orig_host=www.ncbi.nlm.nih.gov'>26000041</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01588-jcav06p0511g005.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®Tissue array analysis of TGF-β and PTHrP expression in HCC and non-cancerous specimens. A human tissue array containing 38 HCC cancer tissues and 9 non-neoplastic liver tissues was double-stained for PTHrP and TGF-β by immunohistochemistry using the Poly-HRP Anti-IgG Detection System (brown staining - TGF-β) and the Biotin SP-AP Detection System (blue staining - PTHrP), respectively. The nuclei were counterstained with nuclear fast red. (A) A representative image showing PTHrP staining but no TGF-β staining in the HCC cancer cells in a HCC patient (ID #08). (B) TGF-β was intensely stained in the matched non-neoplastic liver tissue of the same patient. (C) PTHrP expression level was considerably higher in HCC tissues than in the non-neoplastic liver tissues. (D) TGF-β expression was high in the non-neoplastic liver tissues but low in HCC cancer tissues. Original magnification: x400. Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4439935/&orig_host=www.ncbi.nlm.nih.gov'>26000041</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01588-pth1r-primary-antibodies-fcm-testing-2.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-Parathyroid Hormone Receptor 1/PTH1R antibody (A01588). Overlay histogram showing HEL cells stained with A01588 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Parathyroid Hormone Receptor 1/PTH1R Antibody (A01588, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dep1-ptprj-picoband-trade-antibody-a02319-2-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02319-2-ptprj-primary-antibodies-wb-testing-1.jpgAnti-DEP1/PTPRJ Antibody Picoband® Western blot analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (A02319-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEP1/PTPRJ antigen affinity purified polyclonal antibody (Catalog # A02319-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DEP1/PTPRJ at approximately 240 kDa. The expected band size for DEP1/PTPRJ is at 146 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02319-2-ptprj-primary-antibodies-ihc-testing-2.jpgAnti-DEP1/PTPRJ Antibody Picoband® IHC analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (A02319-2). DEP1/PTPRJ was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DEP1/PTPRJ Antibody (A02319-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02319-2-ptprj-primary-antibodies-ihc-testing-3.jpgAnti-DEP1/PTPRJ Antibody Picoband® IHC analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (A02319-2). DEP1/PTPRJ was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DEP1/PTPRJ Antibody (A02319-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02319-2-ptprj-primary-antibodies-fcm-testing-4.jpgAnti-DEP1/PTPRJ Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-DEP1/PTPRJ antibody (A02319-2). Overlay histogram showing HEL cells stained with A02319-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DEP1/PTPRJ Antibody (A02319-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02319-2-ptprj-primary-antibodies-if-testing-5.jpgAnti-DEP1/PTPRJ Antibody Picoband® IF analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (A02319-2). DEP1/PTPRJ was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DEP1/PTPRJ Antibody (A02319-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rab23-picoband-trade-antibody-a04593-2-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04593-2-rab23-primary-antibodies-wb-testing-1.jpgAnti-RAB23 Antibody Picoband® Western blot analysis of RAB23 using anti-RAB23 antibody (A04593-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB23 antigen affinity purified polyclonal antibody (Catalog # A04593-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB23 at approximately 27 kDa. The expected band size for RAB23 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04593-2-rab23-primary-antibodies-fcm-testing-2.jpgAnti-RAB23 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RAB23 antibody (A04593-2). Overlay histogram showing MCF-7 cells stained with A04593-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB23 Antibody (A04593-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04593-2-rab23-primary-antibodies-fcm-testing-3.jpgAnti-RAB23 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-RAB23 antibody (A04593-2). Overlay histogram showing SiHa cells stained with A04593-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB23 Antibody (A04593-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rgs7-picoband-trade-antibody-a04305-1-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04305-1-rgs7-primary-antibodies-wb-testing-1.jpgAnti-RGS7 Antibody Picoband® Western blot analysis of RGS7 using anti-RGS7 antibody (A04305-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SK-N-SH whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS7 antigen affinity purified polyclonal antibody (Catalog # A04305-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS7 at approximately 65 kDa(h) and 58 kDa(mr). The expected band size for RGS7 is at 58 kDa(h), 55 kDa(m) and 56 kDa(r).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04305-1-rgs7-primary-antibodies-if-testing-2.jpgAnti-RGS7 Antibody Picoband® IF analysis of RGS7 using anti-RGS7 antibody (A04305-1). RGS7 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RGS7 Antibody (A04305-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04305-1-rgs7-primary-antibodies-fcm-testing-3.jpgAnti-RGS7 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-RGS7 antibody (A04305-1). Overlay histogram showing 293T cells stained with A04305-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS7 Antibody (A04305-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-satb2-picoband-trade-antibody-a02588-2-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02588-2-satb2-primary-antibodies-wb-testing-1.jpgAnti-SATB2 Antibody Picoband® Western blot analysis of SATB2 using anti-SATB2 antibody (A02588-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB2 antigen affinity purified polyclonal antibody (Catalog # A02588-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SATB2 at approximately 90 kDa. The expected band size for SATB2 is at 83 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-eaat1-slc1a3-picoband-trade-antibody-a02133-1-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-1-slc1a3-primary-antibodies-wb-testing-1.jpgAnti-EAAT1/SLC1A3 Antibody Picoband® Western blot analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT1/SLC1A3 antigen affinity purified polyclonal antibody (Catalog # A02133-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT1/SLC1A3 at approximately 55 kDa. The expected band size for EAAT1/SLC1A3 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-1-slc1a3-primary-antibodies-ihc-testing-2.jpgAnti-EAAT1/SLC1A3 Antibody Picoband® IHC analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). EAAT1/SLC1A3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EAAT1/SLC1A3 Antibody (A02133-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-1-slc1a3-primary-antibodies-ihc-testing-3.jpgAnti-EAAT1/SLC1A3 Antibody Picoband® IHC analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). EAAT1/SLC1A3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EAAT1/SLC1A3 Antibody (A02133-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-1-slc1a3-primary-antibodies-ihc-testing-4.jpgAnti-EAAT1/SLC1A3 Antibody Picoband® IHC analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). EAAT1/SLC1A3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EAAT1/SLC1A3 Antibody (A02133-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-1-slc1a3-primary-antibodies-ihc-testing-5.jpgAnti-EAAT1/SLC1A3 Antibody Picoband® IHC analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). EAAT1/SLC1A3 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EAAT1/SLC1A3 Antibody (A02133-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-1-slc1a3-primary-antibodies-if-testing-6.jpgAnti-EAAT1/SLC1A3 Antibody Picoband® IF analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). EAAT1/SLC1A3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EAAT1/SLC1A3 Antibody (A02133-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02133-1-slc1a3-primary-antibodies-if-testing-7.jpgAnti-EAAT1/SLC1A3 Antibody Picoband® IF analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). EAAT1/SLC1A3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EAAT1/SLC1A3 Antibody (A02133-1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-taar6-picoband-trade-antibody-a10349-1-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10349-1-taar6-primary-antibodies-wb-testing-1.jpgAnti-TAAR6 Antibody Picoband® Western blot analysis of TARC/CCL17/TAAR6 using anti-TARC/CCL17/TAAR6 antibody (A10349-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARC/CCL17/TAAR6 antigen affinity purified polyclonal antibody (Catalog # A10349-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TARC/CCL17/TAAR6 at approximately 38 kDa. The expected band size for TARC/CCL17/TAAR6 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10349-1-taar6-primary-antibodies-fcm-testing-2.jpgAnti-TAAR6 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TAAR6 antibody (A10349-1). Overlay histogram showing HEL cells stained with A10349-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TAAR6 Antibody (A10349-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dp2-tfdp2-picoband-trade-antibody-a06850-3-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06850-3-tfdp2-primary-antibodies-wb-testing-1.jpgAnti-DP2/TFDP2 Antibody Picoband® Western blot analysis of TFDP2 using anti-TFDP2 antibody (A06850-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFDP2 antigen affinity purified polyclonal antibody (Catalog # A06850-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFDP2 at approximately 49 kDa. The expected band size for TFDP2 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tnni1-picoband-trade-antibody-a07080-1-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07080-1-tnni1-primary-antibodies-wb-testing-1.jpgAnti-TNNI1 Antibody Picoband® Western blot analysis of TNNI1 using anti-TNNI1 antibody (A07080-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI1 antigen affinity purified polyclonal antibody (Catalog # A07080-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI1 at approximately 22 kDa. The expected band size for TNNI1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07080-1-tnni1-primary-antibodies-ihc-testing-2.jpgAnti-TNNI1 Antibody Picoband® IHC analysis of TNNI1 using anti-TNNI1 antibody (A07080-1). TNNI1 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNNI1 Antibody (A07080-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-cardiac-troponin-i-tnni3-picoband-trade-antibody-a01720-3-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01720-3-tnni3-primary-antibodies-wb-testing-1.jpgAnti-Cardiac Troponin I/TNNI3 Antibody Picoband® Western blot analysis of Cardiac Troponin I/TNNI3 using anti-Cardiac Troponin I/TNNI3 antibody (A01720-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin I/TNNI3 antigen affinity purified polyclonal antibody (Catalog # A01720-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin I/TNNI3 at approximately 24 kDa. The expected band size for Cardiac Troponin I/TNNI3 is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tomm20l-picoband-trade-antibody-a18568-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18568-tomm20l-primary-antibodies-wb-testing-1.jpgAnti-TOMM20L Antibody Picoband® Western blot analysis of TOMM20L using anti-TOMM20L antibody (A18568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM20L antigen affinity purified polyclonal antibody (Catalog # A18568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM20L at approximately 21 kDa. The expected band size for TOMM20L is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18568-tomm20l-primary-antibodies-fcm-testing-2.jpgAnti-TOMM20L Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-TOMM20L antibody (A18568). Overlay histogram showing U937 cells stained with A18568 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM20L Antibody (A18568, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tomm40-picoband-trade-antibody-a03166-2-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-wb-testing-1.jpgAnti-TOMM40 Antibody Picoband® Western blot analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM40 antigen affinity purified polyclonal antibody (Catalog # A03166-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM40 at approximately 38 kDa. The expected band size for TOMM40 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-ihc-testing-2.jpgAnti-TOMM40 Antibody Picoband® IHC analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-ihc-testing-3.jpgAnti-TOMM40 Antibody Picoband® IHC analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-ihc-testing-4.jpgAnti-TOMM40 Antibody Picoband® IHC analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-ihc-testing-5.jpgAnti-TOMM40 Antibody Picoband® IHC analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-ihc-testing-6.jpgAnti-TOMM40 Antibody Picoband® IHC analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-ihc-testing-7.jpgAnti-TOMM40 Antibody Picoband® IHC analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-if-testing-8.jpgAnti-TOMM40 Antibody Picoband® IF analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-fcm-testing-9.jpgAnti-TOMM40 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TOMM40 antibody (A03166-2). Overlay histogram showing MCF-7 cells stained with A03166-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM40 Antibody (A03166-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03166-2-tomm40-primary-antibodies-if-testing-10.jpgAnti-TOMM40 Antibody Picoband® IF analysis of TOMM40 using anti-TOMM40 antibody (A03166-2). TOMM40 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TOMM40 Antibody (A03166-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tyk2-picoband-trade-antibody-a01642-3-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01642-3-tyk2-primary-antibodies-wb-testing-1.jpgAnti-TYK2 Antibody Picoband® Western blot analysis of TYK2 using anti-TYK2 antibody (A01642-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYK2 antigen affinity purified polyclonal antibody (Catalog # A01642-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYK2 at approximately 134 kDa. The expected band size for TYK2 is at 134 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01642-3-tyk2-primary-antibodies-fcm-testing-2.jpgAnti-TYK2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TYK2 antibody (A01642-3). Overlay histogram showing MCF-7 cells stained with A01642-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TYK2 Antibody (A01642-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-usp26-picoband-trade-antibody-a07732-1-boster.html2026-03-17T05:13:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07732-1-usp26-primary-antibodies-wb-testing-1.jpgAnti-USP26 Antibody Picoband® Western blot analysis of USP26 using anti-USP26 antibody (A07732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP26 antigen affinity purified polyclonal antibody (Catalog # A07732-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP26 at approximately 104 kDa. The expected band size for USP26 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07732-1-usp26-primary-antibodies-ihc-testing-2.jpgAnti-USP26 Antibody Picoband® IHC analysis of USP26 using anti-USP26 antibody (A07732-1). USP26 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-USP26 Antibody (A07732-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07732-1-usp26-primary-antibodies-ihc-testing-3.jpgAnti-USP26 Antibody Picoband® IHC analysis of USP26 using anti-USP26 antibody (A07732-1). USP26 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-USP26 Antibody (A07732-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07732-1-usp26-primary-antibodies-ihc-testing-4.jpgAnti-USP26 Antibody Picoband® IHC analysis of USP26 using anti-USP26 antibody (A07732-1). USP26 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-USP26 Antibody (A07732-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07732-1-usp26-primary-antibodies-fcm-testing-5.jpgAnti-USP26 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-USP26 antibody (A07732-1). Overlay histogram showing 293T cells stained with A07732-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP26 Antibody (A07732-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-vcp-picoband-trade-antibody-a00610-2-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-wb-testing-1.jpgAnti-VCP Antibody Picoband® Western blot analysis of VCP using anti-VCP antibody (A00610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: Monkey COS-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human placenta tissue lysates, Lane 8: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # A00610-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-ihc-testing-5.jpgAnti-VCP Antibody Picoband®IHC analysis of VCP using anti-VCP antibody (A00610-2). VCP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (A00610-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-wb-testing-2.jpgAnti-VCP Antibody Picoband® Western blot analysis of VCP using anti-VCP antibody (A00610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # A00610-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-ihc-testing-3.jpgAnti-VCP Antibody Picoband® IHC analysis of VCP using anti-VCP antibody (A00610-2). VCP was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (A00610-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-ihc-testing-4.jpgAnti-VCP Antibody Picoband® IHC analysis of VCP using anti-VCP antibody (A00610-2). VCP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (A00610-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-fcm-testing-5.jpgAnti-VCP Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-VCP antibody (A00610-2). Overlay histogram showing MCF-7 cells stained with A00610-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (A00610-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-fcm-testing-6.jpgAnti-VCP Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-VCP antibody (A00610-2). Overlay histogram showing U87 cells stained with A00610-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (A00610-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-fcm-testing-7.jpgAnti-VCP Antibody Picoband® Flow Cytometry analysis of EL-4 cells using anti-VCP antibody (A00610-2). Overlay histogram showing EL-4 cells stained with A00610-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (A00610-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00610-2-vcp-primary-antibodies-fcm-testing-8.jpgAnti-VCP Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-VCP antibody (A00610-2). Overlay histogram showing C6 cells stained with A00610-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (A00610-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-vps53-picoband-trade-antibody-a06836-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-wb-testing-1.jpgAnti-VPS53 Antibody Picoband® Western blot analysis of VPS53 using anti-VPS53 antibody (A06836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS53 antigen affinity purified polyclonal antibody (Catalog # A06836-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS53 at approximately 100 kDa. The expected band size for VPS53 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-ihc-testing-2.jpgAnti-VPS53 Antibody Picoband® IHC analysis of VPS53 using anti-VPS53 antibody (A06836-1). VPS53 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPS53 Antibody (A06836-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-ihc-testing-3.jpgAnti-VPS53 Antibody Picoband® IHC analysis of VPS53 using anti-VPS53 antibody (A06836-1). VPS53 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPS53 Antibody (A06836-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-ihc-testing-4.jpgAnti-VPS53 Antibody Picoband® IHC analysis of VPS53 using anti-VPS53 antibody (A06836-1). VPS53 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPS53 Antibody (A06836-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-ihc-testing-5.jpgAnti-VPS53 Antibody Picoband® IHC analysis of VPS53 using anti-VPS53 antibody (A06836-1). VPS53 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPS53 Antibody (A06836-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-if-testing-6.jpgAnti-VPS53 Antibody Picoband® IF analysis of VPS53 using anti-VPS53 antibody (A06836-1). VPS53 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-VPS53 Antibody (A06836-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-fcm-testing-7.jpgAnti-VPS53 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-VPS53 antibody (A06836-1). Overlay histogram showing U87 cells stained with A06836-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS53 Antibody (A06836-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06836-1-vps53-primary-antibodies-if-testing-8.jpgAnti-VPS53 Antibody Picoband® IF analysis of VPS53 using anti-VPS53 antibody (A06836-1). VPS53 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-VPS53 Antibody (A06836-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-vrk2-picoband-trade-antibody-a04821-2-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04821-2-vrk2-primary-antibodies-wb-testing-1.jpgAnti-VRK2 Antibody Picoband® Western blot analysis of VRK2 using anti-VRK2 antibody (A04821-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRK2 antigen affinity purified polyclonal antibody (Catalog # A04821-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRK2 at approximately 50 kDa. The expected band size for VRK2 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04821-2-vrk2-primary-antibodies-fcm-testing-2.jpgAnti-VRK2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-VRK2 antibody (A04821-2). Overlay histogram showing HL-60 cells stained with A04821-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VRK2 Antibody (A04821-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fens1-wdfy1-picoband-trade-antibody-a10318-3-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10318-3-wdfy1-primary-antibodies-wb-testing-1.jpgAnti-FENS1/WDFY1 Antibody Picoband® Western blot analysis of FENS1/WDFY1 using anti-FENS1/WDFY1 antibody (A10318-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FENS1/WDFY1 antigen affinity purified polyclonal antibody (Catalog # A10318-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FENS1/WDFY1 at approximately 46 kDa. The expected band size for FENS1/WDFY1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10318-3-wdfy1-primary-antibodies-fcm-testing-2.jpgAnti-FENS1/WDFY1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-FENS1/WDFY1 antibody (A10318-3). Overlay histogram showing HL-60 cells stained with A10318-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FENS1/WDFY1 Antibody (A10318-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wdr12-picoband-trade-antibody-a07492-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07492-1-wdr12-primary-antibodies-wb-testing-1.jpgAnti-WDR12 Antibody Picoband® Western blot analysis of WDR12 using anti-WDR12 antibody (A07492-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR12 antigen affinity purified polyclonal antibody (Catalog # A07492-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR12 at approximately 48 kDa. The expected band size for WDR12 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07492-1-wdr12-primary-antibodies-fcm-testing-2.jpgAnti-WDR12 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-WDR12 antibody (A07492-1). Overlay histogram showing HL-60 cells stained with A07492-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR12 Antibody (A07492-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wdr46-picoband-trade-antibody-a12816-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12816-1-wdr46-primary-antibodies-wb-testing-1.jpgAnti-WDR46 Antibody Picoband® Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12816-1-wdr46-primary-antibodies-fcm-testing-2.jpgAnti-WDR46 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-WDR12 antibody (A12816-1). Overlay histogram showing HL-60 cells stained with A12816-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR12 Antibody (A12816-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ccxcr1-xcr1-picoband-trade-antibody-a04185-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04185-1-xcr1-primary-antibodies-wb-testing-1.jpgAnti-CCXCR1/XCR1 Antibody Picoband® Western blot analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCXCR1/XCR1 antigen affinity purified polyclonal antibody (Catalog # A04185-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCXCR1/XCR1 at approximately 42 kDa. The expected band size for CCXCR1/XCR1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04185-1-10020_2024_1059_fig1_html.pngAnti-CCXCR1/XCR1 Antibody Picoband®cDC1s in SCI patients and mouse model of SCI. A PBMCs were collected from 10 healthy volunteers and 10 SCI patients. Identification of cDC1s and cDC2s subsets was performed by flow cytometry. CD11C + HLA-DR + represents cDCs, while CD141 + represents sDC1s and CD1c + represents cDC2 cells. Difference was calculated by Mann–Whitney U test. ** P < 0.01 vs. healthy group. B Mice were divided into sham ( n = 6) and SCI ( n = 6) groups. BMS score detection was performed. Difference was calculated by two-way ANOVA test. ** P < 0.01 vs. sham group. C–D The positive expressions of MHCII and XCR1 in spinal cord tissues were detected by IF assay. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01 vs. sham group. SCI : spinal cord injury; dpi : days post-injury; cDC : conventional dendritic cell Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-024-01059-4'>39901071</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04185-1-10020_2024_1059_fig2_html.pngAnti-CCXCR1/XCR1 Antibody Picoband®cDC1 depletion reduces CD8 + T cell infiltration after SCI injury. A Mice were divided into SCI ( n = 6) and SCI + Qu ( n = 6) groups. Qu administration was started on day 14 after surgery. B BMS score was determined. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01 vs. SCI group. C Positive expression of XCR1 in spinal cord tissues was detected by IF assay. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01. D The results of flow cytometry of XCR1 + cDCs in lymph nodes and blood. The cDC population was expressed as CD11C and I-A/I-E (MHCII) double positive, and XCR1 + represents XCR1 + cDCs. E The proportion of CD8 + T cells in spinal cord tissues was determined by flow cytometry. CD3 + T cells were circled with CD3 gate, and further CD3 + CD8 + T cells were analyzed. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01 vs. SCI group. N = 6. F ELISA was used to detect IFN-γ levels in spinal cord tissues. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01 vs. SCI group. N = 6. dpi : days post-injury; Qu : Quizartinib Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-024-01059-4'>39901071</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04185-1-10020_2024_1059_fig4_html.pngAnti-CCXCR1/XCR1 Antibody Picoband®Excess cDC1s in non-lymphoid tissues in LNs of SCI mice is due to migration of cDC1s from the spinal cord. A Leukocyte (CD45 + ) and pre-DC subsets in bone marrow of sham and SCI groups were detected by flow cytometry. Immune cells were circled by CD45 + and murine pre-DCs were further detected by CD135 + CD11c + . The difference was calculated by unpaired two-tailed T-test. N = 6. B–C Non-lymphoid tissue cDC1 (CD11C + I-A/I-E + CD103 + ) proportion and lymphoid-resident cDC1 (CD11C + I-A/I-E + CD8α + ) subsets in dcLN and LLN of sham and SCI groups were detected by flow cytometry. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01 vs. SCI group. N = 3. D cDC1s (CD11C + I-A/I-E + CD103 + ) and cDC2s (CD11C + I-A/I-E + CD11b + ) subsets in the blood of SCI-3 dpi and SCI-42 dpi groups were detected by flow cytometry. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01 vs. SCI group. N = 6. E cDC1 subsets (CD45 + XCR1 + CD103 + ) in spinal cord tissues of SCI-3 dpi and SCI-42 dpi groups were detected by flow cytometry. The difference was calculated by unpaired two-tailed T-test. ** P < 0.01 vs. SCI group. N = 6. F The positive expressions of CD11C and LYVE-1 in the spinal cord tissues of SCI group mice were detected by IF assay. BM : bone marrow; dcLN : deep cervical lymph nodes; LLN : lumbar lymph nodes Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-024-01059-4'>39901071</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04185-1-xcr1-primary-antibodies-ihc-testing-2.jpgAnti-CCXCR1/XCR1 Antibody Picoband® IHC analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1). CCXCR1/XCR1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCXCR1/XCR1 Antibody (A04185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04185-1-xcr1-primary-antibodies-ihc-testing-3.jpgAnti-CCXCR1/XCR1 Antibody Picoband® IHC analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1). CCXCR1/XCR1 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCXCR1/XCR1 Antibody (A04185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04185-1-xcr1-primary-antibodies-ihc-testing-4.jpgAnti-CCXCR1/XCR1 Antibody Picoband® IHC analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1). CCXCR1/XCR1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCXCR1/XCR1 Antibody (A04185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ythdc2-picoband-trade-antibody-a09692-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09692-1-ythdc2-primary-antibodies-wb-testing-1.jpgAnti-YTHDC2 Antibody Picoband® Western blot analysis of YTHDC2 using anti-YTHDC2 antibody (A09692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YTHDC2 antigen affinity purified polyclonal antibody (Catalog # A09692-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YTHDC2 at approximately 175 kDa. The expected band size for YTHDC2 is at 160 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09692-1-ythdc2-primary-antibodies-if-testing-2.jpgAnti-YTHDC2 Antibody Picoband® IF analysis of YTHDC2 using anti-YTHDC2 antibody (A09692-1). YTHDC2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-YTHDC2 Antibody (A09692-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09692-1-ythdc2-primary-antibodies-fcm-testing-3.jpgAnti-YTHDC2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-YTHDC2 antibody (A09692-1). Overlay histogram showing MCF-7 cells stained with A09692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YTHDC2 Antibody (A09692-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09692-1-ythdc2-primary-antibodies-fcm-testing-4.jpgAnti-YTHDC2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-YTHDC2 antibody (A09692-1). Overlay histogram showing SiHa cells stained with A09692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YTHDC2 Antibody (A09692-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-zmym1-picoband-trade-antibody-a18075-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18075-1-zmym1-primary-antibodies-wb-testing-1.jpgAnti-ZMYM1 Antibody Picoband® Western blot analysis of ZMYM1 using anti-ZMYM1 antibody (A18075-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZMYM1 antigen affinity purified polyclonal antibody (Catalog # A18075-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZMYM1 at approximately 150 kDa. The expected band size for ZMYM1 is at 129 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18075-1-zmym1-primary-antibodies-fcm-testing-2.jpgAnti-ZMYM1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-ZMYM1 antibody (A18075-1). Overlay histogram showing HL-60 cells stained with A18075-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZMYM1 Antibody (A18075-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ramp-zmym2-picoband-trade-antibody-a04648-1-boster.html2026-04-06T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04648-1-zmym2-primary-antibodies-wb-testing-1.jpgAnti-RAMP/ZMYM2 Antibody Picoband® Western blot analysis of RAMP/ZMYM2 using anti-RAMP/ZMYM2 antibody (A04648-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse EL-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAMP/ZMYM2 antigen affinity purified polyclonal antibody (Catalog # A04648-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAMP/ZMYM2 at approximately 150 kDa. The expected band size for RAMP/ZMYM2 is at 155 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04648-1-zmym2-primary-antibodies-if-testing-2.jpgAnti-RAMP/ZMYM2 Antibody Picoband® IF analysis of RAMP/ZMYM2 using anti-RAMP/ZMYM2 antibody (A04648-1). RAMP/ZMYM2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAMP/ZMYM2 Antibody (A04648-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04648-1-zmym2-primary-antibodies-fcm-testing-3.jpgAnti-RAMP/ZMYM2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RAMP/ZMYM2 antibody (A04648-1). Overlay histogram showing HL-60 cells stained with A04648-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAMP/ZMYM2 Antibody (A04648-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-acaca-picoband-trade-antibody-a01802-3-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01802-3-acaca-primary-antibodies-wb-testing-1.jpgAnti-ACACA Antibody Picoband® Western blot analysis of ACACA using anti-ACACA antibody (A01802-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACACA antigen affinity purified polyclonal antibody (Catalog # A01802-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACACA at approximately 266 kDa. The expected band size for ACACA is at 266 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01802-3-acaca-primary-antibodies-if-testing-2.jpgAnti-ACACA Antibody Picoband® IF analysis of ACACA using anti-ACACA antibody (A01802-3). ACACA was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ACACA Antibody (A01802-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01802-3-acaca-primary-antibodies-fcm-testing-3.jpgAnti-ACACA Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-ACACA antibody (A01802-3). Overlay histogram showing U251 cells stained with A01802-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACACA Antibody (A01802-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-acss2-picoband-trade-antibody-a02809-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02809-1-acss2-primary-antibodies-wb-testing-1_1.jpgAnti-ACSS2 Antibody Picoband®Western blot analysis of ACSS2 using anti-ACSS2 antibody (A02809-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSS2 antigen affinity purified polyclonal antibody (Catalog # A02809-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSS2 at approximately 79 kDa. The expected band size for ACSS2 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02809-1-acss2-primary-antibodies-ihc-testing-1.jpgAnti-ACSS2 Antibody Picoband®IHC analysis of ACSS2 using anti-ACSS2 antibody (A02809-1). ACSS2 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACSS2 Antibody (A02809-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02809-1-acss2-primary-antibodies-ihc-testing-2.jpgAnti-ACSS2 Antibody Picoband®IHC analysis of ACSS2 using anti-ACSS2 antibody (A02809-1). ACSS2 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACSS2 Antibody (A02809-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02809-1-acss2-primary-antibodies-fcm-testing-1.jpgAnti-ACSS2 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-ACSS2 antibody (A02809-1). Overlay histogram showing MCF-7 cells stained with A02809-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSS2 Antibody (A02809-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-adam22-picoband-trade-antibody-a05660-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05660-1-adam22-primary-antibodies-wb-testing-1.jpgAnti-ADAM22 Antibody Picoband® Western blot analysis of ADAM22 using anti-ADAM22 antibody (A05660-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM22 antigen affinity purified polyclonal antibody (Catalog # A05660-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM22 at approximately 80-90 kDa. The expected band size for ADAM22 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05660-1-adam22-primary-antibodies-ihc-testing-2_1.jpgAnti-ADAM22 Antibody Picoband® IHC analysis of ADAM22 using anti-ADAM22 antibody (A05660-1). ADAM22 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAM22 Antibody (A05660-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05660-1-adam22-primary-antibodies-ihc-testing-3.jpgAnti-ADAM22 Antibody Picoband® IHC analysis of ADAM22 using anti-ADAM22 antibody (A05660-1). ADAM22 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAM22 Antibody (A05660-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05660-1-adam22-primary-antibodies-fcm-testing-4.jpgAnti-ADAM22 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ADAM22 antibody (A05660-1). Overlay histogram showing RT4 cells stained with A05660-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAM22 Antibody (A05660-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-adgrg1-picoband-trade-antibody-a05578-2-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05578-2-adgrg1-primary-antibodies-wb-testing-1.jpgAnti-Adgrg1 Antibody Picoband® Western blot analysis of Adgrg1 using anti-Adgrg1 antibody (A05578-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mosue heart tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adgrg1 antigen affinity purified polyclonal antibody (Catalog # A05578-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adgrg1 at approximately 78 kDa. The expected band size for Adgrg1 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05578-2-adgrg1-primary-antibodies-ihc-testing-2.jpgAnti-Adgrg1 Antibody Picoband® IHC analysis of Adgrg1 using anti-Adgrg1 antibody (A05578-2). Adgrg1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Adgrg1 Antibody (A05578-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05578-2-adgrg1-primary-antibodies-ihc-testing-3.jpgAnti-Adgrg1 Antibody Picoband® IHC analysis of Adgrg1 using anti-Adgrg1 antibody (A05578-2). Adgrg1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Adgrg1 Antibody (A05578-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05578-2-adgrg1-primary-antibodies-fcm-testing-4.jpgAnti-Adgrg1 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-Adgrg1 antibody (A05578-2). Overlay histogram showing RAW264.7 cells stained with A05578-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Adgrg1 Antibody (A05578-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd83-picoband-trade-antibody-a01777-1-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01777-1-adam22-primary-antibodies-wb-testing-1.jpgAnti-CD83 Antibody Picoband® Western blot analysis of ADAM22 using anti-ADAM22 antibody (A01777-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM22 antigen affinity purified polyclonal antibody (Catalog # A01777-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM22 at approximately 45 kDa. The expected band size for ADAM22 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01777-1-adam22-primary-antibodies-fcm-testing-2.jpgAnti-CD83 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ADAM22 antibody (A01777-1). Overlay histogram showing RT4 cells stained with A01777-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAM22 Antibody (A01777-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hsp40-dnajb1-picoband-trade-antibody-a03100-2-boster.html2026-03-17T05:13:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03100-2-dnajb1-primary-antibodies-wb-testing-1.jpgAnti-Hsp40/DNAJB1 Antibody Picoband® Western blot analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp40/DNAJB1 antigen affinity purified polyclonal antibody (Catalog # A03100-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp40/DNAJB1 at approximately 38 kDa. The expected band size for Hsp40/DNAJB1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03100-2-dnajb1-primary-antibodies-fcm-testing-2.jpgAnti-Hsp40/DNAJB1 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-Hsp40/DNAJB1 antibody (A03100-2). Overlay histogram showing Daudi cells stained with A03100-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp40/DNAJB1 Antibody (A03100-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-epoxide-hydrolase-ephx1-picoband-trade-antibody-a00899-1-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00899-1-ephx1-primary-antibodies-wb-testing-1.jpgAnti-Epoxide hydrolase/EPHX1 Antibody Picoband® Western blot analysis of Epoxide hydrolase/EPHX1 using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epoxide hydrolase/EPHX1 antigen affinity purified polyclonal antibody (Catalog # A00899-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epoxide hydrolase/EPHX1 at approximately 53 kDa. The expected band size for Epoxide hydrolase/EPHX1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00899-1-ephx1-primary-antibodies-fcm-testing-2.jpgAnti-Epoxide hydrolase/EPHX1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1). Overlay histogram showing RT4 cells stained with A00899-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epoxide hydrolase/EPHX1 Antibody (A00899-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00899-1-ephx1-primary-antibodies-fcm-testing-3_1.jpgAnti-Epoxide hydrolase/EPHX1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1). Overlay histogram showing A549 cells stained with A00899-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epoxide hydrolase/EPHX1 Antibody (A00899-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00899-1-ephx1-primary-antibodies-fcm-testing-4.jpgAnti-Epoxide hydrolase/EPHX1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Epoxide hydrolase/EPHX1 antibody (A00899-1). Overlay histogram showing SiHa cells stained with A00899-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epoxide hydrolase/EPHX1 Antibody (A00899-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-erp29-picoband-trade-antibody-a03621-2-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-wb-testing-1.jpgAnti-ERP29 Antibody Picoband® Western blot analysis of ERP29 using anti-ERP29 antibody (A03621-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERP29 antigen affinity purified polyclonal antibody (Catalog # A03621-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERP29 at approximately 29 kDa. The expected band size for ERP29 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-ihc-testing-2.jpgAnti-ERP29 Antibody Picoband® IHC analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-ihc-testing-3.jpgAnti-ERP29 Antibody Picoband® IHC analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-ihc-testing-4.jpgAnti-ERP29 Antibody Picoband® IHC analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-ihc-testing-5.jpgAnti-ERP29 Antibody Picoband® IHC analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-ihc-testing-6.jpgAnti-ERP29 Antibody Picoband® IHC analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in a paraffin-embedded section of mouse pulmonary lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-ihc-testing-7.jpgAnti-ERP29 Antibody Picoband® IHC analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-if-testing-8.jpgAnti-ERP29 Antibody Picoband® IF analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-fcm-testing-9.jpgAnti-ERP29 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-ERP29 antibody (A03621-2). Overlay histogram showing THP-1 cells stained with A03621-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERP29 Antibody (A03621-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03621-2-erp29-primary-antibodies-if-testing-10.jpgAnti-ERP29 Antibody Picoband® IF analysis of ERP29 using anti-ERP29 antibody (A03621-2). ERP29 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ERP29 Antibody (A03621-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fip1l1-picoband-trade-antibody-a02452-1-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-wb-testing-1.jpgAnti-FIP1L1 Antibody Picoband® Western blot analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIP1L1 antigen affinity purified polyclonal antibody (Catalog # A02452-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIP1L1 at approximately 72 kDa. The expected band size for FIP1L1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-ihc-testing-2.jpgAnti-FIP1L1 Antibody Picoband® IHC analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-ihc-testing-3.jpgAnti-FIP1L1 Antibody Picoband® IHC analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in a paraffin-embedded section of high-grade serous carcinoma of human ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-ihc-testing-4.jpgAnti-FIP1L1 Antibody Picoband® IHC analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-ihc-testing-5.jpgAnti-FIP1L1 Antibody Picoband® IHC analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-ihc-testing-6.jpgAnti-FIP1L1 Antibody Picoband® IHC analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-if-testing-7.jpgAnti-FIP1L1 Antibody Picoband® IF analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-fcm-testing-8.jpgAnti-FIP1L1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-FIP1L1 antibody (A02452-1). Overlay histogram showing HepG2 cells stained with A02452-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FIP1L1 Antibody (A02452-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-if-testing-9.jpgAnti-FIP1L1 Antibody Picoband® IF analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02452-1-fip1l1-primary-antibodies-if-testing-10.jpgAnti-FIP1L1 Antibody Picoband® IF analysis of FIP1L1 using anti-FIP1L1 antibody (A02452-1). FIP1L1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FIP1L1 Antibody (A02452-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-heme-oxygenase-1-hmox1-picoband-trade-antibody-a00253-2-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00253-2-hmox1-primary-antibodies-wb-testing-1.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband® Western blot analysis of Heme Oxygenase 1/HMOX1 using anti-Heme Oxygenase 1/HMOX1 antibody (A00253-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/HMOX1 antigen affinity purified polyclonal antibody (Catalog # A00253-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/HMOX1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/HMOX1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00253-2-hmox1-primary-antibodies-fcm-testing-2.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-Heme Oxygenase 1/HMOX1 antibody (A00253-2). Overlay histogram showing U20S cells stained with A00253-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Heme Oxygenase 1/HMOX1 Antibody (A00253-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hoxc12-picoband-trade-antibody-a15490-1-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15490-1-hoxc12-primary-antibodies-wb-testing-1.jpgAnti-HOXC12 Antibody Picoband® Western blot analysis of HOXC12 using anti-HOXC12 antibody (A15490-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXC12 antigen affinity purified polyclonal antibody (Catalog # A15490-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXC12 at approximately 35 kDa. The expected band size for HOXC12 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15490-1-hoxc12-primary-antibodies-ihc-testing-2.jpgAnti-HOXC12 Antibody Picoband® IHC analysis of HOXC12 using anti-HOXC12 antibody (A15490-1). HOXC12 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXC12 Antibody (A15490-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-hsd17b7-picoband-trade-antibody-a05850-1-boster.html2026-03-17T05:16:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05850-1-hsd17b7-primary-antibodies-wb-testing-1_1.jpgAnti-HSD17B7 Antibody Picoband® Western blot analysis of HSD17B7 using anti-HSD17B7 antibody (A05850-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B7 antigen affinity purified polyclonal antibody (Catalog # A05850-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B7 at approximately 38 kDa. The expected band size for HSD17B7 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05850-1-hsd17b7-primary-antibodies-ihc-testing-2.jpgAnti-HSD17B7 Antibody Picoband® IHC analysis of HSD17B7 using anti-HSD17B7 antibody (A05850-1). HSD17B7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD17B7 Antibody (A05850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05850-1-hsd17b7-primary-antibodies-ihc-testing-3.jpgAnti-HSD17B7 Antibody Picoband® IHC analysis of HSD17B7 using anti-HSD17B7 antibody (A05850-1). HSD17B7 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD17B7 Antibody (A05850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05850-1-hsd17b7-primary-antibodies-ihc-testing-4.jpgAnti-HSD17B7 Antibody Picoband® IHC analysis of HSD17B7 using anti-HSD17B7 antibody (A05850-1). HSD17B7 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD17B7 Antibody (A05850-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05850-1-hsd17b7-primary-antibodies-if-testing-5.jpgAnti-HSD17B7 Antibody Picoband® IF analysis of HSD17B7 using anti-HSD17B7 antibody (A05850-1). HSD17B7 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HSD17B7 Antibody (A05850-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05850-1-hsd17b7-primary-antibodies-fcm-testing-6.jpgAnti-HSD17B7 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-HSD17B7 antibody (A05850-1). Overlay histogram showing JK cells stained with A05850-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B7 Antibody (A05850-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hsd17b8-picoband-trade-antibody-a08741-1-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08741-1-hsd17b8-primary-antibodies-wb-testing-1.jpgAnti-HSD17B8 Antibody Picoband® Western blot analysis of HSD17B8 using anti-HSD17B8 antibody (A08741-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B8 antigen affinity purified polyclonal antibody (Catalog # A08741-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B8 at approximately 34 kDa. The expected band size for HSD17B8 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08741-1-hsd17b8-primary-antibodies-fcm-testing-2.jpgAnti-HSD17B8 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-HSD17B8 antibody (A08741-1). Overlay histogram showing JK cells stained with A08741-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B8 Antibody (A08741-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-eplin-lima1-picoband-trade-antibody-a05231-1-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05231-1-lima1-primary-antibodies-wb-testing-1.jpgAnti-EPLIN/LIMA1 Antibody Picoband® Western blot analysis of EPLIN/LIMA1 using anti-EPLIN/LIMA1 antibody (A05231-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPLIN/LIMA1 antigen affinity purified polyclonal antibody (Catalog # A05231-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPLIN/LIMA1 at approximately 85 kDa and 100 kDa. The expected band size for EPLIN/LIMA1 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05231-1-lima1-primary-antibodies-ihc-testing-2.jpgAnti-EPLIN/LIMA1 Antibody Picoband® IHC analysis of EPLIN/LIMA1 using anti-EPLIN/LIMA1 antibody (A05231-1). EPLIN/LIMA1 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPLIN/LIMA1 Antibody (A05231-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05231-1-lima1-primary-antibodies-ihc-testing-3.jpgAnti-EPLIN/LIMA1 Antibody Picoband® IHC analysis of EPLIN/LIMA1 using anti-EPLIN/LIMA1 antibody (A05231-1). EPLIN/LIMA1 was detected in a paraffin-embedded section of human oncocytoma of the right kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPLIN/LIMA1 Antibody (A05231-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05231-1-lima1-primary-antibodies-if-testing-4.jpgAnti-EPLIN/LIMA1 Antibody Picoband® IF analysis of EPLIN/LIMA1 using anti-EPLIN/LIMA1 antibody (A05231-1). EPLIN/LIMA1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EPLIN/LIMA1 Antibody (A05231-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05231-1-lima1-primary-antibodies-fcm-testing-5.jpgAnti-EPLIN/LIMA1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-EPLIN/LIMA1 antibody (A05231-1). Overlay histogram showing THP-1 cells stained with A05231-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPLIN/LIMA1 Antibody (A05231-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mat1a-picoband-trade-antibody-a04203-1-boster.html2026-03-17T05:13:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04203-1-mat1a-primary-antibodies-wb-testing-1_1.jpgAnti-MAT1A Antibody Picoband®Western blot analysis of MAT1A using anti-MAT1A antibody (A04203-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT1A antigen affinity purified polyclonal antibody (Catalog # A04203-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAT1A at approximately 50 kDa. The expected band size for MAT1A is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04203-1-mat1a-primary-antibodies-if-testing-1.jpgAnti-MAT1A Antibody Picoband®IF analysis of MAT1A using anti-MAT1A antibody (A04203-1) and anti-Alpha Tubulin antibody (M03989-3). MAT1A was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MAT1A Antibody (A04203-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04203-1-mat1a-primary-antibodies-fcm-testing-1.jpgAnti-MAT1A Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-MAT1A antibody (A04203-1). Overlay histogram showing Jurkat cells stained with A04203-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAT1A Antibody (A04203-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ncoa4-picoband-trade-antibody-a04368-3-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04368-3-ncoa4-primary-antibodies-wb-testing-1.jpgAnti-NCOA4 Antibody Picoband® Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (Catalog # A04368-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04368-3-1-s2.0-s0211699525001183-gr2.jpgAnti-NCOA4 Antibody Picoband®IRN inhibits ferroptosis in db/db mice. (A–C) Examination of iron content, MDA levels, and GSH levels in mouse renal tissues. (D–I) Evaluation of SLC7A11, GPX4, TFR-1, FTH-1, and NCOA4 protein expression in mouse kidney tissues through western blotting and densitometric analysis of the bands. Results are presented as the mean ± SD of 6 mice. ***p < 0.001 versus db/m; ###p < 0.001 versus db/db. Index in NEFROLOGIA under a CC BY license. DOI: <a href='https://www.sciencedirect.com/science/article/pii/S0211699525001183'>10.1016/j.nefro.2025.501408</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04368-3-1-s2.0-s0211699525001183-gr3.jpgAnti-NCOA4 Antibody Picoband®IRN curbs HG-induced HK-2 cell injury and ferroptosis. (A) Detection of the cytotoxicity of IRN against HK-2 cells through CCK-8 assay. *p < 0.05. (B) Assessment of HK-2 cell viability after treatment with normal glucose, normal glucose + mannitol, high glucose, or high glucose + IRN (5, 10, 25, or 50 μM) by CCK-8 assay. (C–E) Measurement of iron content, MDA levels, and GSH levels in HK-2 cells. (F–K) Estimation of SLC7A11, GPX4, TFR-1, FTH-1, and NCOA4 protein levels in HK-2 cells via western blotting and densitometric analysis of the bands. Results are presented as the mean ± SD of three independent experiments. ***p < 0.001 versus NG; #p < 0.05, ##p < 0.01, ###p < 0.001 versus HG. Index in NEFROLOGIA under a CC BY license. DOI: <a href='https://www.sciencedirect.com/science/article/pii/S0211699525001183'>10.1016/j.nefro.2025.501408</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04368-3-ncoa4-primary-antibodies-wb-testing-2.jpgAnti-NCOA4 Antibody Picoband® Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (Catalog # A04368-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04368-3-ncoa4-primary-antibodies-fcm-testing-3.jpgAnti-NCOA4 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-NCOA4 antibody (A04368-3). Overlay histogram showing HL-60 cells stained with A04368-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA4 Antibody (A04368-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufs5-picoband-trade-antibody-a04093-1-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-wb-testing-1_1.jpgAnti-NDUFS5 Antibody Picoband® Western blot analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS5 antigen affinity purified polyclonal antibody (Catalog # A04093-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS5 at approximately 15 kDa. The expected band size for NDUFS5 is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-ihc-testing-2_1.jpgAnti-NDUFS5 Antibody Picoband® IHC analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). NDUFS5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS5 Antibody (A04093-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-ihc-testing-3_1.jpgAnti-NDUFS5 Antibody Picoband® IHC analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). NDUFS5 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS5 Antibody (A04093-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-ihc-testing-4_1.jpgAnti-NDUFS5 Antibody Picoband® IHC analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). NDUFS5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS5 Antibody (A04093-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-ihc-testing-5_1.jpgAnti-NDUFS5 Antibody Picoband® IHC analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). NDUFS5 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS5 Antibody (A04093-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-ihc-testing-6.jpgAnti-NDUFS5 Antibody Picoband® IHC analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). NDUFS5 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS5 Antibody (A04093-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-fcm-testing-8.jpgAnti-NDUFS5 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-NDUFS5 antibody (A04093-1). Overlay histogram showing Hela cells stained with A04093-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS5 Antibody (A04093-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04093-1-ndufs5-primary-antibodies-if-testing-7.jpgAnti-NDUFS5 Antibody Picoband® IF analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). NDUFS5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFS5 Antibody (A04093-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-erp72-pdia4-picoband-trade-antibody-a07267-1-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07267-1-pdia4-primary-antibodies-fcm-testing-2_1.jpgAnti-ERp72/PDIA4 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-ERp72/PDIA4 antibody (A07267-1). Overlay histogram showing Hela cells stained with A07267-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERp72/PDIA4 Antibody (A07267-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07267-1-pdia4-primary-antibodies-fcm-testing-3_1.jpgAnti-ERp72/PDIA4 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-ERp72/PDIA4 antibody (A07267-1). Overlay histogram showing THP-1 cells stained with A07267-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERp72/PDIA4 Antibody (A07267-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07267-1-pdia4-primary-antibodies-wb-testing-1_1.jpgAnti-ERp72/PDIA4 Antibody Picoband® Western blot analysis of ERp72/PDIA4 using anti-ERp72/PDIA4 antibody (A07267-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PANC-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp72/PDIA4 antigen affinity purified polyclonal antibody (Catalog # A07267-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp72/PDIA4 at approximately 72 kDa. The expected band size for ERp72/PDIA4 is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-poglut3-picoband-trade-antibody-a15507-1-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15507-1-poglut3-primary-antibodies-wb-testing-1.jpgAnti-POGLUT3 Antibody Picoband® Western blot analysis of POGLUT3 using anti-POGLUT3 antibody (A15507-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGLUT3 antigen affinity purified polyclonal antibody (Catalog # A15507-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGLUT3 at approximately 68 kDa. The expected band size for POGLUT3 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15507-1-poglut3-primary-antibodies-ihc-testing-1.jpgAnti-POGLUT3 Antibody Picoband®IHC analysis of KDELC2/POGLUT3 using anti-KDELC2/POGLUT3 antibody (A15507-1). KDELC2/POGLUT3 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KDELC2/POGLUT3 Antibody (A15507-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15507-1-poglut3-primary-antibodies-if-testing-2.jpgAnti-POGLUT3 Antibody Picoband® IF analysis of POGLUT3 using anti-POGLUT3 antibody (A15507-1). POGLUT3 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POGLUT3 Antibody (A15507-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15507-1-poglut3-primary-antibodies-fcm-testing-3.jpgAnti-POGLUT3 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-POGLUT3 antibody (A15507-1). Overlay histogram showing U251 cells stained with A15507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POGLUT3 Antibody (A15507-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gadd34-ppp1r15a-picoband-trade-antibody-a02394-4-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02394-4-ppp1r15a-primary-antibodies-wb-testing-1.jpgAnti-GADD34/Ppp1r15a Antibody Picoband® Western blot analysis of GADD34/Ppp1r15a using anti-GADD34/Ppp1r15a antibody (A02394-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/Ppp1r15a antigen affinity purified polyclonal antibody (Catalog # A02394-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/Ppp1r15a at approximately 100 kDa. The expected band size for GADD34/Ppp1r15a is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02394-4-ppp1r15a-primary-antibodies-fcm-testing-2.jpgAnti-GADD34/Ppp1r15a Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-GADD34/Ppp1r15a antibody (A02394-4). Overlay histogram showing ANA-1 cells stained with A02394-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GADD34/Ppp1r15a Antibody (A02394-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rdh10-picoband-trade-antibody-a08376-1-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08376-1-rdh10-primary-antibodies-wb-testing-1.jpgAnti-RDH10 Antibody Picoband® Western blot analysis of RDH10 using anti-RDH10 antibody (A08376-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RDH10 antigen affinity purified polyclonal antibody (Catalog # A08376-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RDH10 at approximately 38 kDa. The expected band size for RDH10 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08376-1-rdh10-primary-antibodies-fcm-testing-2.jpgAnti-RDH10 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-RDH10 antibody (A08376-1). Overlay histogram showing PC-3 cells stained with A08376-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RDH10 Antibody (A08376-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mst3-stk24-picoband-trade-antibody-a04112-3-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04112-3-stk24-primary-antibodies-wb-testing-1.jpgAnti-MST3/STK24 Antibody Picoband® Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (A04112-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (Catalog # A04112-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04112-3-stk24-primary-antibodies-ihc-testing-5.jpgAnti-MST3/STK24 Antibody Picoband®IHC analysis of STK24 using anti-STK24 antibody (A04112-3). STK24 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STK24 Antibody (A04112-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04112-3-stk24-primary-antibodies-wb-testing-2_1.jpgAnti-MST3/STK24 Antibody Picoband® Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (A04112-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse small intestin tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse brian tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (Catalog # A04112-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04112-3-stk24-primary-antibodies-ihc-testing-3.jpgAnti-MST3/STK24 Antibody Picoband® IHC analysis of MST3/STK24 using anti-MST3/STK24 antibody (A04112-3). MST3/STK24 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MST3/STK24 Antibody (A04112-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04112-3-stk24-primary-antibodies-ihc-testing-4.jpgAnti-MST3/STK24 Antibody Picoband® IHC analysis of MST3/STK24 using anti-MST3/STK24 antibody (A04112-3). MST3/STK24 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MST3/STK24 Antibody (A04112-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04112-3-stk24-primary-antibodies-fcm-testing-5.jpgAnti-MST3/STK24 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-MST3/STK24 antibody (A04112-3). Overlay histogram showing U251 cells stained with A04112-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MST3/STK24 Antibody (A04112-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mst4-stk26-picoband-trade-antibody-a09552-2-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09552-2-stk26-primary-antibodies-wb-testing-1.jpgAnti-MST4/STK26 Antibody Picoband® Western blot analysis of MST4/STK26 using anti-MST4/STK26 antibody (A09552-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST4/STK26 antigen affinity purified polyclonal antibody (Catalog # A09552-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST4/STK26 at approximately 52 kDa. The expected band size for MST4/STK26 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09552-2-stk26-primary-antibodies-ihc-testing-1.jpgAnti-MST4/STK26 Antibody Picoband®IHC analysis of MST4/STK26 using anti-MST4/STK26 antibody (A09552-2). MST4/STK26 was detected in a paraffin-embedded section of human ovarican cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MST4/STK26 Antibody (A09552-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09552-2-stk26-primary-antibodies-ihc-testing-2.jpgAnti-MST4/STK26 Antibody Picoband®IHC analysis of MST4/STK26 using anti-MST4/STK26 antibody (A09552-2). MST4/STK26 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MST4/STK26 Antibody (A09552-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09552-2-stk26-primary-antibodies-fcm-testing-2.jpgAnti-MST4/STK26 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-MST4/STK26 antibody (A09552-2). Overlay histogram showing MCF-7 cells stained with A09552-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MST4/STK26 Antibody (A09552-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tbx18-picoband-trade-antibody-a06044-2-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-wb-testing-1.jpgAnti-TBX18 Antibody Picoband® Western blot analysis of TBX18 using anti-TBX18 antibody (A06044-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX18 antigen affinity purified polyclonal antibody (Catalog # A06044-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBX18 at approximately 70 kDa. The expected band size for TBX18 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-ihc-testing-2.jpgAnti-TBX18 Antibody Picoband® IHC analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-ihc-testing-3.jpgAnti-TBX18 Antibody Picoband® IHC analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-ihc-testing-4.jpgAnti-TBX18 Antibody Picoband® IHC analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-ihc-testing-5.jpgAnti-TBX18 Antibody Picoband® IHC analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human moderately differentiated adenocarcinoma of the cervix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-ihc-testing-6.jpgAnti-TBX18 Antibody Picoband® IHC analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-ihc-testing-7.jpgAnti-TBX18 Antibody Picoband® IHC analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-ihc-testing-8.jpgAnti-TBX18 Antibody Picoband® IHC analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-if-testing-9.jpgAnti-TBX18 Antibody Picoband® IF analysis of TBX18 using anti-TBX18 antibody (A06044-2). TBX18 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TBX18 Antibody (A06044-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06044-2-tbx18-primary-antibodies-fcm-testing-10.jpgAnti-TBX18 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TBX18 antibody (A06044-2). Overlay histogram showing U87 cells stained with A06044-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBX18 Antibody (A06044-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tdg-picoband-trade-antibody-a15317-1-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15317-1-tdg-primary-antibodies-wb-testing-1_1.jpgAnti-TDG Antibody Picoband®Western blot analysis of TDG using anti-TDG antibody (A15317-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDG antigen affinity purified polyclonal antibody (A15317-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TDG at approximately 55-60 kDa. The expected band size for TDG is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15317-1-tdg-primary-antibodies-if-testing-1.jpgAnti-TDG Antibody Picoband®IF analysis of TDG using anti-TDG antibody (A15317-1) and anti-Tubulin Alpha antibody (M03989-3). TDG was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TDG Antibody (A15317-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15317-1-tdg-primary-antibodies-fcm-testing-1.jpgAnti-TDG Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TDG antibody (A15317-1). Overlay histogram showing MCF-7 cells stained with A15317-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDG Antibody (A15317-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tdp2-picoband-trade-antibody-a01804-1-boster.html2026-03-17T05:13:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01804-1-tdp2-primary-antibodies-wb-testing-1.jpgAnti-TDP2 Antibody Picoband® Western blot analysis of TDP2 using anti-TDP2 antibody (A01804-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP2 antigen affinity purified polyclonal antibody (Catalog # A01804-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TDP2 at approximately 49 kDa. The expected band size for TDP2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01804-1-tdp2-primary-antibodies-if-testing-2.jpgAnti-TDP2 Antibody Picoband® IF analysis of TDP2 using anti-TDP2 antibody (A01804-1). TDP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TDP2 Antibody (A01804-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-trk-fused-gene-tfg-picoband-trade-antibody-a02870-3-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-wb-testing-1_1.jpgAnti-TRK fused gene/TFG Antibody Picoband® Western blot analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human Hacat whole cell lysates, Lane 8: human T-47D whole cell lysates, Lane 9: rat pancreas tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse small intestine tissue lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRK fused gene/TFG antigen affinity purified polyclonal antibody (Catalog # A02870-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRK fused gene/TFG at approximately 60 kDa. The expected band size for TRK fused gene/TFG is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-ihc-testing-2_1.jpgAnti-TRK fused gene/TFG Antibody Picoband® IHC analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-ihc-testing-3.jpgAnti-TRK fused gene/TFG Antibody Picoband® IHC analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-ihc-testing-4.jpgAnti-TRK fused gene/TFG Antibody Picoband® IHC analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-ihc-testing-5.jpgAnti-TRK fused gene/TFG Antibody Picoband® IHC analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-ihc-testing-6.jpgAnti-TRK fused gene/TFG Antibody Picoband® IHC analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-ihc-testing-7.jpgAnti-TRK fused gene/TFG Antibody Picoband® IHC analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-if-testing-8.jpgAnti-TRK fused gene/TFG Antibody Picoband® IF analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02870-3-tfg-primary-antibodies-if-testing-9.jpgAnti-TRK fused gene/TFG Antibody Picoband® IF analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). TRK fused gene/TFG was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRK fused gene/TFG Antibody (A02870-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tgif-tgif1-picoband-trade-antibody-a04122-1-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04122-1-tgif1-primary-antibodies-wb-testing-1.jpgAnti-TGIF/TGIF1 Antibody Picoband® Western blot analysis of TGIF/TGIF1 using anti-TGIF/TGIF1 antibody (A04122-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGIF/TGIF1 antigen affinity purified polyclonal antibody (Catalog # A04122-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGIF/TGIF1 at approximately 43 kDa. The expected band size for TGIF/TGIF1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04122-1-tgif1-primary-antibodies-fcm-testing-2.jpgAnti-TGIF/TGIF1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-TGIF/TGIF1 antibody (A04122-1). Overlay histogram showing PC-3 cells stained with A04122-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGIF/TGIF1 Antibody (A04122-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-thap11-picoband-trade-antibody-a08519-2-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-wb-testing-1.jpgAnti-THAP11 Antibody Picoband® Western blot analysis of THAP11 using anti-THAP11 antibody (A08519-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THAP11 antigen affinity purified polyclonal antibody (Catalog # A08519-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THAP11 at approximately 50 kDa. The expected band size for THAP11 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-wb-testing-2.jpgAnti-THAP11 Antibody Picoband® Western blot analysis of THAP11 using anti-THAP11 antibody (A08519-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THAP11 antigen affinity purified polyclonal antibody (Catalog # A08519-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THAP11 at approximately 50 kDa. The expected band size for THAP11 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-ihc-testing-3.jpgAnti-THAP11 Antibody Picoband® IHC analysis of THAP11 using anti-THAP11 antibody (A08519-2). THAP11 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THAP11 Antibody (A08519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-ihc-testing-4.jpgAnti-THAP11 Antibody Picoband® IHC analysis of THAP11 using anti-THAP11 antibody (A08519-2). THAP11 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THAP11 Antibody (A08519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-ihc-testing-5.jpgAnti-THAP11 Antibody Picoband® IHC analysis of THAP11 using anti-THAP11 antibody (A08519-2). THAP11 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THAP11 Antibody (A08519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-ihc-testing-6.jpgAnti-THAP11 Antibody Picoband® IHC analysis of THAP11 using anti-THAP11 antibody (A08519-2). THAP11 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THAP11 Antibody (A08519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-if-testing-7.jpgAnti-THAP11 Antibody Picoband® IF analysis of THAP11 using anti-THAP11 antibody (A08519-2). THAP11 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-THAP11 Antibody (A08519-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08519-2-thap11-primary-antibodies-fcm-testing-8.jpgAnti-THAP11 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-THAP11 antibody (A08519-2). Overlay histogram showing MCF-7 cells stained with A08519-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THAP11 Antibody (A08519-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-thbs3-picoband-trade-antibody-a11652-2-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11652-2-thbs3-primary-antibodies-wb-testing-1.jpgAnti-THBS3 Antibody Picoband® Western blot analysis of THBS3 using anti-THBS3 antibody (A11652-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THBS3 antigen affinity purified polyclonal antibody (Catalog # A11652-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THBS3 at approximately 120 kDa. The expected band size for THBS3 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11652-2-thbs3-primary-antibodies-ihc-testing-2.jpgAnti-THBS3 Antibody Picoband® IHC analysis of THBS3 using anti-THBS3 antibody (A11652-2). THBS3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THBS3 Antibody (A11652-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11652-2-thbs3-primary-antibodies-ihc-testing-3.jpgAnti-THBS3 Antibody Picoband® IHC analysis of THBS3 using anti-THBS3 antibody (A11652-2). THBS3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THBS3 Antibody (A11652-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11652-2-thbs3-primary-antibodies-fcm-testing-4.jpgAnti-THBS3 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-THBS3 antibody (A11652-2). Overlay histogram showing MCF-7 cells stained with A11652-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THBS3 Antibody (A11652-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-themis-picoband-trade-antibody-a07854-3-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07854-3-themis-primary-antibodies-wb-testing-1.jpgAnti-THEMIS Antibody Picoband® Western blot analysis of THEMIS using anti-THEMIS antibody (A07854-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THEMIS antigen affinity purified polyclonal antibody (Catalog # A07854-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THEMIS at approximately 73 kDa. The expected band size for THEMIS is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07854-3-themis-primary-antibodies-fcm-testing-2.jpgAnti-THEMIS Antibody Picoband® Flow Cytometry analysis of JK cells using anti-THEMIS antibody (A07854-3). Overlay histogram showing JK cells stained with A07854-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THEMIS Antibody (A07854-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-thumpd3-picoband-trade-antibody-a15721-1-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15721-1-thumpd3-primary-antibodies-wb-testing-1.jpgAnti-THUMPD3 Antibody Picoband® Western blot analysis of THUMPD3 using anti-THUMPD3 antibody (A15721-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THUMPD3 antigen affinity purified polyclonal antibody (Catalog # A15721-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THUMPD3 at approximately 68 kDa. The expected band size for THUMPD3 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15721-1-thumpd3-primary-antibodies-fcm-testing-2.jpgAnti-THUMPD3 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-THUMPD3 antibody (A15721-1). Overlay histogram showing PC-3 cells stained with A15721-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THUMPD3 Antibody (A15721-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tigd1-picoband-trade-antibody-a17411-1-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-wb-testing-1.jpgAnti-TIGD1 Antibody Picoband® Western blot analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGD1 antigen affinity purified polyclonal antibody (Catalog # A17411-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIGD1 at approximately 68-70 kDa. The expected band size for TIGD1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-ihc-testing-2.jpgAnti-TIGD1 Antibody Picoband® IHC analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-ihc-testing-3.jpgAnti-TIGD1 Antibody Picoband® IHC analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-ihc-testing-4.jpgAnti-TIGD1 Antibody Picoband® IHC analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-ihc-testing-5.jpgAnti-TIGD1 Antibody Picoband® IHC analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-ihc-testing-6.jpgAnti-TIGD1 Antibody Picoband® IHC analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-ihc-testing-7.jpgAnti-TIGD1 Antibody Picoband® IHC analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-if-testing-8.jpgAnti-TIGD1 Antibody Picoband® IF analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-fcm-testing-9.jpgAnti-TIGD1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-TIGD1 antibody (A17411-1). Overlay histogram showing PC-3 cells stained with A17411-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIGD1 Antibody (A17411-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17411-1-tigd1-primary-antibodies-if-testing-10.jpgAnti-TIGD1 Antibody Picoband® IF analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). TIGD1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIGD1 Antibody (A17411-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tigd3-picoband-trade-antibody-a19473-2-boster.html2026-03-17T05:13:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a19473-2-tigd3-primary-antibodies-wb-testing-1.jpgAnti-TIGD3 Antibody Picoband® Western blot analysis of TIGD3 using anti-TIGD3 antibody (A19473-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGD3 antigen affinity purified polyclonal antibody (Catalog # A19473-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIGD3 at approximately 60 kDa. The expected band size for TIGD3 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a19473-2-tigd3-primary-antibodies-fcm-testing-2.jpgAnti-TIGD3 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TIGD3 antibody (A19473-2). Overlay histogram showing Daudi cells stained with A19473-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIGD3 Antibody (A19473-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tigd6-picoband-trade-antibody-a18145-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18145-tigd6-primary-antibodies-wb-testing-1.jpgAnti-TIGD6 Antibody Picoband® Western blot analysis of TIGD6 using anti-TIGD6 antibody (A18145). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGD6 antigen affinity purified polyclonal antibody (Catalog # A18145) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIGD6 at approximately 59 kDa. The expected band size for TIGD6 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18145-tigd6-primary-antibodies-fcm-testing-2.jpgAnti-TIGD6 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TIGD6 antibody (A18145). Overlay histogram showing Daudi cells stained with A18145 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIGD6 Antibody (A18145, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tim-4-timd4-picoband-trade-antibody-a06637-2-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06637-2-timd4-primary-antibodies-wb-testing-1.jpgAnti-TIM 4/TIMD4 Antibody Picoband® Western blot analysis of TIM 4/TIMD4 using anti-TIM 4/TIMD4 antibody (A06637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 4/TIMD4 antigen affinity purified polyclonal antibody (Catalog # A06637-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 4/TIMD4 at approximately 42 kDa. The expected band size for TIM 4/TIMD4 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-timeless-picoband-trade-antibody-a00831-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00831-timeless-primary-antibodies-wb-testing-1.jpgAnti-TIMELESS Antibody Picoband® Western blot analysis of TIMELESS using anti-TIMELESS antibody (A00831). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMELESS antigen affinity purified polyclonal antibody (Catalog # A00831) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMELESS at approximately 160 kDa. The expected band size for TIMELESS is at 138 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tjap1-picoband-trade-antibody-a12692-1-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12692-1-tjap1-primary-antibodies-wb-testing-1.jpgAnti-TJAP1 Antibody Picoband® Western blot analysis of TJAP1 using anti-TJAP1 antibody (A12692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJAP1 antigen affinity purified polyclonal antibody (Catalog # A12692-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJAP1 at approximately 86 kDa. The expected band size for TJAP1 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12692-1-tjap1-primary-antibodies-ihc-testing-1.jpgAnti-TJAP1 Antibody Picoband®IHC analysis of TJAP1 using anti-TJAP1 antibody (A12692-1). TJAP1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TJAP1 Antibody (A12692-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12692-1-tjap1-primary-antibodies-if-testing-2.jpgAnti-TJAP1 Antibody Picoband® IF analysis of TJAP1 using anti-TJAP1 antibody (A12692-1). TJAP1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TJAP1 Antibody (A12692-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12692-1-tjap1-primary-antibodies-fcm-testing-3.jpgAnti-TJAP1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TJAP1 antibody (A12692-1). Overlay histogram showing U87 cells stained with A12692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TJAP1 Antibody (A12692-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tlr4-picoband-trade-antibody-a00017-3-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00017-3-tlr4-primary-antibodies-wb-testing-1.jpgAnti-Tlr4 Antibody Picoband® Western blot analysis of Tlr4 using anti-Tlr4 antibody (A00017-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr4 antigen affinity purified polyclonal antibody (Catalog # A00017-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr4 at approximately 96 kDa. The expected band size for Tlr4 is at 96 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tlr5-picoband-trade-antibody-a00462-2-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00462-2-tlr5-primary-antibodies-wb-testing-1.jpgAnti-Tlr5 Antibody Picoband® Western blot analysis of Tlr5 using anti-Tlr5 antibody (A00462-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr5 antigen affinity purified polyclonal antibody (Catalog # A00462-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr5 at approximately 100 kDa. The expected band size for Tlr5 is at 98 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tm6sf2-picoband-trade-antibody-a02832-2-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02832-2-tm6sf2-primary-antibodies-wb-testing-1.jpgAnti-TM6SF2 Antibody Picoband® Western blot analysis of TM6SF2 using anti-TM6SF2 antibody (A02832-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 3: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM6SF2 antigen affinity purified polyclonal antibody (Catalog # A02832-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM6SF2 at approximately 43 kDa. The expected band size for TM6SF2 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tm9sf3-picoband-trade-antibody-a12868-1-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12868-1-tm9sf3-primary-antibodies-wb-testing-1.jpgAnti-TM9SF3 Antibody Picoband® Western blot analysis of TM9SF3 using anti-TM9SF3 antibody (A12868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM9SF3 antigen affinity purified polyclonal antibody (Catalog # A12868-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM9SF3 at approximately 72 kDa. The expected band size for TM9SF3 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tmem97-picoband-trade-antibody-a08336-1-boster.html2026-03-17T05:13:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08336-1-tmem97-primary-antibodies-wb-testing-1.jpgAnti-TMEM97 Antibody Picoband® Western blot analysis of TMEM97 using anti-TMEM97 antibody (A08336-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM97 antigen affinity purified polyclonal antibody (Catalog # A08336-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM97 at approximately 23 kDa. The expected band size for TMEM97 is at 21 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-uba5-picoband-trade-antibody-a06385-2-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06385-2-uba5-primary-antibodies-wb-testing-1.jpgAnti-UBA5 Antibody Picoband® Western blot analysis of UBA5 using anti-UBA5 antibody (A06385-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pamcreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA5 antigen affinity purified polyclonal antibody (Catalog # A06385-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA5 at approximately 50 kDa. The expected band size for UBA5 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06385-2-uba5-primary-antibodies-ihc-testing-2.jpgAnti-UBA5 Antibody Picoband® IHC analysis of UBA5 using anti-UBA5 antibody (A06385-2). UBA5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBA5 Antibody (A06385-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06385-2-uba5-primary-antibodies-ihc-testing-3.jpgAnti-UBA5 Antibody Picoband® IHC analysis of UBA5 using anti-UBA5 antibody (A06385-2). UBA5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBA5 Antibody (A06385-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06385-2-uba5-primary-antibodies-ip-testing-1.jpgAnti-UBA5 Antibody Picoband®Immunoprecipitating UBA5 in Jurkat whole cell lysate. Western blot analysis of UBA5 using anti-UBA5 antibody (A06385-2). Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-UBA5 antibody in Jurkat whole cell lysate, Lane 3: anti-UBA5 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBA5 antigen affinity purified polyclonal antibody (A06385-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UBA5 at approximately 50 kDa. The expected band size for UBA5 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06385-2-uba5-primary-antibodies-fcm-testing-4.jpgAnti-UBA5 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-UBA5 antibody (A06385-2). Overlay histogram showing PC-3 cells stained with A06385-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBA5 Antibody (A06385-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-uba6-picoband-trade-antibody-a03213-2-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03213-2-uba6-primary-antibodies-wb-testing-1.jpgAnti-UBA6 Antibody Picoband® Western blot analysis of UBA6 using anti-UBA6 antibody (A03213-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA6 antigen affinity purified polyclonal antibody (Catalog # A03213-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA6 at approximately 118 kDa. The expected band size for UBA6 is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03213-2-uba6-primary-antibodies-ihc-testing-2.jpgAnti-UBA6 Antibody Picoband® IHC analysis of UBA6 using anti-UBA6 antibody (A03213-2). UBA6 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBA6 Antibody (A03213-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03213-2-uba6-primary-antibodies-ihc-testing-3.jpgAnti-UBA6 Antibody Picoband® IHC analysis of UBA6 using anti-UBA6 antibody (A03213-2). UBA6 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBA6 Antibody (A03213-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03213-2-uba6-primary-antibodies-ihc-testing-4.jpgAnti-UBA6 Antibody Picoband® IHC analysis of UBA6 using anti-UBA6 antibody (A03213-2). UBA6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBA6 Antibody (A03213-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03213-2-uba6-primary-antibodies-fcm-testing-5.jpgAnti-UBA6 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-UBA6 antibody (A03213-2). Overlay histogram showing PC-3 cells stained with A03213-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBA6 Antibody (A03213-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ubc12-ube2m-picoband-trade-antibody-a07478-1-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-wb-testing-1.jpgAnti-UBC12/UBE2M Antibody Picoband® Western blot analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC12/UBE2M antigen affinity purified polyclonal antibody (Catalog # A07478-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC12/UBE2M at approximately 21 kDa. The expected band size for UBC12/UBE2M is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-ihc-testing-2.jpgAnti-UBC12/UBE2M Antibody Picoband® IHC analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). UBC12/UBE2M was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBC12/UBE2M Antibody (A07478-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-ihc-testing-3.jpgAnti-UBC12/UBE2M Antibody Picoband® IHC analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). UBC12/UBE2M was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBC12/UBE2M Antibody (A07478-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-ihc-testing-4.jpgAnti-UBC12/UBE2M Antibody Picoband® IHC analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). UBC12/UBE2M was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBC12/UBE2M Antibody (A07478-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-ihc-testing-5.jpgAnti-UBC12/UBE2M Antibody Picoband® IHC analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). UBC12/UBE2M was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBC12/UBE2M Antibody (A07478-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-fcm-testing-7.jpgAnti-UBC12/UBE2M Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-UBC12/UBE2M antibody (A07478-1). Overlay histogram showing Daudi cells stained with A07478-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBC12/UBE2M Antibody (A07478-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-if-testing-6.jpgAnti-UBC12/UBE2M Antibody Picoband® IF analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). UBC12/UBE2M was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-UBC12/UBE2M Antibody (A07478-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-if-testing-8.jpgAnti-UBC12/UBE2M Antibody Picoband® IF analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). UBC12/UBE2M was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-UBC12/UBE2M Antibody (A07478-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07478-1-ube2m-primary-antibodies-if-testing-9.jpgAnti-UBC12/UBE2M Antibody Picoband® IF analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody (A07478-1). UBC12/UBE2M was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-UBC12/UBE2M Antibody (A07478-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ubc13-ube2n-picoband-trade-antibody-a02462-2-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-wb-testing-1.jpgAnti-Ubc13/UBE2N Antibody Picoband® Western blot analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse small intestine tissue lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubc13/UBE2N antigen affinity purified polyclonal antibody (Catalog # A02462-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubc13/UBE2N at approximately 17 kDa. The expected band size for Ubc13/UBE2N is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-ihc-testing-1.jpgAnti-Ubc13/UBE2N Antibody Picoband®IHC analysis of UBC13/UBE2N using anti-UBC13/UBE2N antibody (A02462-2). UBC13/UBE2N was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBC13/UBE2N Antibody (A02462-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-ihc-testing-2.jpgAnti-Ubc13/UBE2N Antibody Picoband® IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Ubc13/UBE2N was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ubc13/UBE2N Antibody (A02462-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-ihc-testing-3.jpgAnti-Ubc13/UBE2N Antibody Picoband® IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Ubc13/UBE2N was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ubc13/UBE2N Antibody (A02462-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-ihc-testing-4.jpgAnti-Ubc13/UBE2N Antibody Picoband® IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Ubc13/UBE2N was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ubc13/UBE2N Antibody (A02462-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-ihc-testing-5.jpgAnti-Ubc13/UBE2N Antibody Picoband® IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Ubc13/UBE2N was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ubc13/UBE2N Antibody (A02462-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-if-testing-6.jpgAnti-Ubc13/UBE2N Antibody Picoband® IF analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Ubc13/UBE2N was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Ubc13/UBE2N Antibody (A02462-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02462-2-ube2n-primary-antibodies-fcm-testing-7.jpgAnti-Ubc13/UBE2N Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-Ubc13/UBE2N antibody (A02462-2). Overlay histogram showing Daudi cells stained with A02462-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ubc13/UBE2N Antibody (A02462-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ube2o-picoband-trade-antibody-a09217-2-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09217-2-ube2o-primary-antibodies-wb-testing-1.jpgAnti-UBE2O Antibody Picoband® Western blot analysis of UBE2O using anti-UBE2O antibody (A09217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2O antigen affinity purified polyclonal antibody (Catalog # A09217-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2O at approximately 200 kDa. The expected band size for UBE2O is at 141 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09217-2-ube2o-primary-antibodies-fcm-testing-2.jpgAnti-UBE2O Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-UBE2O antibody (A09217-2). Overlay histogram showing Daudi cells stained with A09217-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2O Antibody (A09217-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ube2s-picoband-trade-antibody-a03045-2-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03045-2-ube2s-primary-antibodies-wb-testing-1.jpgAnti-UBE2S Antibody Picoband® Western blot analysis of UBE2S using anti-UBE2S antibody (A03045-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2S antigen affinity purified polyclonal antibody (Catalog # A03045-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2S at approximately 24 kDa. The expected band size for UBE2S is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03045-2-ube2s-primary-antibodies-if-testing-2.jpgAnti-UBE2S Antibody Picoband® IF analysis of UBE2S using anti-UBE2S antibody (A03045-2). UBE2S was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-UBE2S Antibody (A03045-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03045-2-ube2s-primary-antibodies-fcm-testing-3.jpgAnti-UBE2S Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-UBE2S antibody (A03045-2). Overlay histogram showing HL-60 cells stained with A03045-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2S Antibody (A03045-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ugt1a1-picoband-trade-antibody-a01865-2-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01865-2-ugt1a1-primary-antibodies-wb-testing-1.jpgAnti-Ugt1a1 Antibody Picoband® Western blot analysis of Ugt1a1 using anti-Ugt1a1 antibody (A01865-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ugt1a1 antigen affinity purified polyclonal antibody (Catalog # A01865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ugt1a1 at approximately 55 kDa. The expected band size for Ugt1a1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01865-2-gr1_lrg.jpgAnti-Ugt1a1 Antibody Picoband®Dose- and age-finding studies in juvenile Ugt1a−/− mice. (A) Scheme of the experimental design. Ugt1a−/− mice were injected either at postnatal day 11 (P11), P25, or P40 with different doses of the AAV8-AAT-hUGT1A1 vector and euthanized at P120. To prevent neonatal mortality, all mice were treated temporarily with phototherapy (PT-light blue rectangle) up to P30. (B and E) Ugt1a−/− mice were injected at P11 with 2.5E11 (n = 4) or 5.0E11 vg/kg (n = 7) of AAV8-AAT-hUGT1A1 vector. Plasma bilirubin (B) and total anti-AAV8 NAbs titer (highest serum dilution giving 50% inhibition of transduction) (E) were determined. Untr (PT), Ugt1a−/− mice control animals treated temporarily with PT n = 8; 2.5E11 vg/kg, AAV8-treated Ugt1a−/− mice n = 4; 5.0E11 vg/kg, AAV8-treated Ugt1a−/− mice n = 7. (C and F) Ugt1a−/− mice were injected at P25 with 1.0E11 (n = 5) or 2.5E11 vg/kg (n = 6) of AAV8-AAT-hUGT1A1 vector. Plasma bilirubin (C) and total anti-AAV8 NAbs titer (F) were determined. AAV8-treated Ugt1a−/− mice n = 5; 2.5E11 vg/kg, AAV8-treated Ugt1a−/− mice n = 6. (D and G) Ugt1a−/− mice were injected at P40 with 1.0E11 (n = 4) or 2.5E11 vg/kg (n = 4) of AAV8-AAT-hUGT1A1 vector. Plasma bilirubin (D) and total anti-AAV8 NAbs titer (G) were determined. Linear mixed-effect model followed by post hoc tests. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. For all the graphs results are presented as mean (SD). Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/molecular-therapy-family/methods/fulltext/S2329-0501(24)00179-7'>39193315</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01865-2-ugt1a1-primary-antibodies-fcm-testing-2.jpgAnti-Ugt1a1 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Ugt1a1 antibody (A01865-2). Overlay histogram showing ANA-1 cells stained with A01865-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ugt1a1 Antibody (A01865-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-usp16-picoband-trade-antibody-a05795-2-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-2-usp16-primary-antibodies-wb-testing-1.jpgAnti-USP16 Antibody Picoband® Western blot analysis of USP16 using anti-USP16 antibody (A05795-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP16 antigen affinity purified polyclonal antibody (Catalog # A05795-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP16 at approximately 110 kDa. The expected band size for USP16 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-2-usp16-primary-antibodies-if-testing-2.jpgAnti-USP16 Antibody Picoband® IF analysis of USP16 using anti-USP16 antibody (A05795-2). USP16 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-USP16 Antibody (A05795-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05795-2-usp16-primary-antibodies-fcm-testing-3.jpgAnti-USP16 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-USP16 antibody (A05795-2). Overlay histogram showing HepG2 cells stained with A05795-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP16 Antibody (A05795-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ffr-vps51-picoband-trade-antibody-a08518-1-boster.html2026-03-17T05:13:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08518-1-vps51-primary-antibodies-wb-testing-1.jpgAnti-FFR/VPS51 Antibody Picoband® Western blot analysis of FFR/VPS51 using anti-FFR/VPS51 antibody (A08518-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FFR/VPS51 antigen affinity purified polyclonal antibody (Catalog # A08518-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FFR/VPS51 at approximately 86 kDa. The expected band size for FFR/VPS51 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08518-1-vps51-primary-antibodies-fcm-testing-2.jpgAnti-FFR/VPS51 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-FFR/VPS51 antibody (A08518-1). Overlay histogram showing U20S cells stained with A08518-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FFR/VPS51 Antibody (A08518-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wdr3-picoband-trade-antibody-a12636-1-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12636-1-wdr3-primary-antibodies-wb-testing-1.jpgAnti-WDR3 Antibody Picoband® Western blot analysis of WDR3 using anti-WDR3 antibody (A12636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR3 antigen affinity purified polyclonal antibody (Catalog # A12636-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR3 at approximately 106 kDa. The expected band size for WDR3 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12636-1-wdr3-primary-antibodies-if-testing-2.jpgAnti-WDR3 Antibody Picoband® IF analysis of WDR3 using anti-WDR3 antibody (A12636-1). WDR3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WDR3 Antibody (A12636-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12636-1-wdr3-primary-antibodies-fcm-testing-3.jpgAnti-WDR3 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-WDR3 antibody (A12636-1). Overlay histogram showing Daudi cells stained with A12636-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR3 Antibody (A12636-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wdr7-picoband-trade-antibody-a14596-1-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12636-1-wdr7-primary-antibodies-wb-testing-1.jpgAnti-WDR7 Antibody Picoband® Western blot analysis of WDR7 using anti-WDR7 antibody (A12636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR7 antigen affinity purified polyclonal antibody (Catalog # A12636-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR7 at approximately 160 kDa. The expected band size for WDR7 is at 164 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14596-1-wdr7-primary-antibodies-ihc-testing-4.jpgAnti-WDR7 Antibody Picoband®IHC analysis of WDR7 using anti-WDR7 antibody (A14596-1). WDR7 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR7 Antibody (A14596-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12636-1-wdr7-primary-antibodies-ihc-testing-2.jpgAnti-WDR7 Antibody Picoband® IHC analysis of WDR7 using anti-WDR7 antibody (A14596-1). WDR7 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR7 Antibody (A14596-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12636-1-wdr7-primary-antibodies-ihc-testing-3.jpgAnti-WDR7 Antibody Picoband® IHC analysis of WDR7 using anti-WDR7 antibody (A14596-1). WDR7 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR7 Antibody (A14596-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12636-1-wdr7-primary-antibodies-fcm-testing-4.jpgAnti-WDR7 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-WDR7 antibody (A14596-1). Overlay histogram showing U20S cells stained with A14596-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR7 Antibody (A14596-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wipf1-picoband-trade-antibody-a04501-2-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04501-2-wipf1-primary-antibodies-wb-testing-1.jpgAnti-WIPF1 Antibody Picoband® Western blot analysis of WIPF1 using anti-WIPF1 antibody (A04501-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WIPF1 antigen affinity purified polyclonal antibody (Catalog # A04501-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WIPF1 at approximately 56 kDa. The expected band size for WIPF1 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04501-2-wipf1-primary-antibodies-fcm-testing-2.jpgAnti-WIPF1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-WIPF1 antibody (A04501-2). Overlay histogram showing HEL cells stained with A04501-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WIPF1 Antibody (A04501-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04501-2-wipf1-primary-antibodies-fcm-testing-3.jpgAnti-WIPF1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-WIPF1 antibody (A04501-2). Overlay histogram showing U87 cells stained with A04501-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WIPF1 Antibody (A04501-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wipf3-picoband-trade-antibody-a14977-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14977-wipf3-primary-antibodies-wb-testing-1.jpgAnti-WIPF3 Antibody Picoband® Western blot analysis of WIPF3 using anti-WIPF3 antibody (A14977). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WIPF3 antigen affinity purified polyclonal antibody (Catalog # A14977) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WIPF3 at approximately 49 kDa. The expected band size for WIPF3 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14977-wipf3-primary-antibodies-fcm-testing-2.jpgAnti-WIPF3 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-WIPF3 antibody (A14977). Overlay histogram showing U20S cells stained with A14977 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WIPF3 Antibody (A14977, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wnt7a-picoband-trade-antibody-a01728-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01728-wnt7a-primary-antibodies-wb-testing-1_1.jpgAnti-WNT7A Antibody Picoband® Western blot analysis of WNT7A using anti-WNT7A antibody (A01728). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT7A antigen affinity purified polyclonal antibody (Catalog # A01728) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for WNT7A at approximately 40 kDa. The expected band size for WNT7A is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-wnt10b-picoband-trade-antibody-a02574-1-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02574-1-wnt10b-primary-antibodies-wb-testing-1.jpgAnti-WNT10B Antibody Picoband® Western blot analysis of WNT10B using anti-WNT10B antibody (A02574-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT10B antigen affinity purified polyclonal antibody (Catalog # A02574-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT10B at approximately 50 kDa. The expected band size for WNT10B is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02574-1-wnt10b-primary-antibodies-fcm-testing-2.jpgAnti-WNT10B Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-WNT10B antibody (A02574-1). Overlay histogram showing PC-3 cells stained with A02574-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT10B Antibody (A02574-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02574-1-wnt10b-primary-antibodies-fcm-testing-3.jpgAnti-WNT10B Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-WNT10B antibody (A02574-1). Overlay histogram showing ANA-1 cells stained with A02574-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT10B Antibody (A02574-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02574-1-wnt10b-primary-antibodies-fcm-testing-4.jpgAnti-WNT10B Antibody Picoband® Flow Cytometry analysis of NRK cells using anti-WNT10B antibody (A02574-1). Overlay histogram showing NRK cells stained with A02574-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT10B Antibody (A02574-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-exportin-5-xpo5-picoband-trade-antibody-a02900-3-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02900-3-xpo5-primary-antibodies-wb-testing-1.jpgAnti-Exportin-5/XPO5 Antibody Picoband® Western blot analysis of Exportin-5/XPO5 using anti-Exportin-5/XPO5 antibody (A02900-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Exportin-5/XPO5 antigen affinity purified polyclonal antibody (Catalog # A02900-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Exportin-5/XPO5 at approximately 130 kDa. The expected band size for Exportin-5/XPO5 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02900-3-xpo5-primary-antibodies-if-testing-2.jpgAnti-Exportin-5/XPO5 Antibody Picoband® IF analysis of Exportin-5/XPO5 using anti-Exportin-5/XPO5 antibody (A02900-3). Exportin-5/XPO5 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Exportin-5/XPO5 Antibody (A02900-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02900-3-xpo5-primary-antibodies-fcm-testing-3.jpgAnti-Exportin-5/XPO5 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-Exportin-5/XPO5 antibody (A02900-3). Overlay histogram showing Daudi cells stained with A02900-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Exportin-5/XPO5 Antibody (A02900-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-msy2-ybox2-ybx2-picoband-trade-antibody-a08610-2-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08610-2-ybx2-primary-antibodies-wb-testing-1.jpgAnti-MSY2/YBOX2/YBX2 Antibody Picoband® Western blot analysis of MSY2/YBOX2/YBX2 using anti-MSY2/YBOX2/YBX2 antibody (A08610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSY2/YBOX2/YBX2 antigen affinity purified polyclonal antibody (Catalog # A08610-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSY2/YBOX2/YBX2 at approximately 55 kDa. The expected band size for MSY2/YBOX2/YBX2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08610-2-ybx2-primary-antibodies-ihc-testing-2.jpgAnti-MSY2/YBOX2/YBX2 Antibody Picoband® IHC analysis of MSY2/YBOX2/YBX2 using anti-MSY2/YBOX2/YBX2 antibody (A08610-2). MSY2/YBOX2/YBX2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSY2/YBOX2/YBX2 Antibody (A08610-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08610-2-ybx2-primary-antibodies-ihc-testing-3.jpgAnti-MSY2/YBOX2/YBX2 Antibody Picoband® IHC analysis of MSY2/YBOX2/YBX2 using anti-MSY2/YBOX2/YBX2 antibody (A08610-2). MSY2/YBOX2/YBX2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSY2/YBOX2/YBX2 Antibody (A08610-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08610-2-ybx2-primary-antibodies-fcm-testing-4.jpgAnti-MSY2/YBOX2/YBX2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-MSY2/YBOX2/YBX2 antibody (A08610-2). Overlay histogram showing MCF-7 cells stained with A08610-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSY2/YBOX2/YBX2 Antibody (A08610-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08610-2-ybx2-primary-antibodies-fcm-testing-5.jpgAnti-MSY2/YBOX2/YBX2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-MSY2/YBOX2/YBX2 antibody (A08610-2). Overlay histogram showing U20S cells stained with A08610-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSY2/YBOX2/YBX2 Antibody (A08610-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-zmynd8-picoband-trade-antibody-a06830-2-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-wb-testing-1.jpgAnti-ZMYND8 Antibody Picoband® Western blot analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZMYND8 antigen affinity purified polyclonal antibody (Catalog # A06830-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZMYND8 at approximately 150 kDa. The expected band size for ZMYND8 is at 132 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-ihc-testing-2.jpgAnti-ZMYND8 Antibody Picoband® IHC analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). ZMYND8 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZMYND8 Antibody (A06830-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-ihc-testing-3.jpgAnti-ZMYND8 Antibody Picoband® IHC analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). ZMYND8 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZMYND8 Antibody (A06830-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-ihc-testing-4.jpgAnti-ZMYND8 Antibody Picoband® IHC analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). ZMYND8 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZMYND8 Antibody (A06830-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-ihc-testing-5.jpgAnti-ZMYND8 Antibody Picoband® IHC analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). ZMYND8 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZMYND8 Antibody (A06830-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-ihc-testing-6.jpgAnti-ZMYND8 Antibody Picoband® IHC analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). ZMYND8 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZMYND8 Antibody (A06830-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-ihc-testing-7.jpgAnti-ZMYND8 Antibody Picoband® IHC analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). ZMYND8 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZMYND8 Antibody (A06830-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-if-testing-8.jpgAnti-ZMYND8 Antibody Picoband® IF analysis of ZMYND8 using anti-ZMYND8 antibody (A06830-2). ZMYND8 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZMYND8 Antibody (A06830-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06830-2-zmynd8-primary-antibodies-fcm-testing-9.jpgAnti-ZMYND8 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-ZMYND8 antibody (A06830-2). Overlay histogram showing Daudi cells stained with A06830-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZMYND8 Antibody (A06830-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-znf609-picoband-trade-antibody-a15674-2-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15674-2-znf609-primary-antibodies-wb-testing-1.jpgAnti-ZNF609 Antibody Picoband® Western blot analysis of ZNF609 using anti-ZNF609 antibody (A15674-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF609 antigen affinity purified polyclonal antibody (Catalog # A15674-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNF609 at approximately 180 kDa. The expected band size for ZNF609 is at 151 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15674-2-znf609-primary-antibodies-if-testing-2.jpgAnti-ZNF609 Antibody Picoband® IF analysis of ZNF609 using anti-ZNF609 antibody (A15674-2). ZNF609 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZNF609 Antibody (A15674-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15674-2-znf609-primary-antibodies-fcm-testing-3.jpgAnti-ZNF609 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-ZNF609 antibody (A15674-2). Overlay histogram showing HepG2 cells stained with A15674-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZNF609 Antibody (A15674-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-znrf3-picoband-trade-antibody-a02974-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02974-1-znrf3-primary-antibodies-wb-testing-1.jpgAnti-ZNRF3 Antibody Picoband® Western blot analysis of ZNRF3 using anti-ZNRF3 antibody (A02974-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNRF3 antigen affinity purified polyclonal antibody (Catalog # A02974-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNRF3 at approximately 130 kDa. The expected band size for ZNRF3 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02974-1-znrf3-primary-antibodies-if-testing-2.jpgAnti-ZNRF3 Antibody Picoband® IF analysis of ZNRF3 using anti-ZNRF3 antibody (A02974-1). ZNRF3 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZNRF3 Antibody (A02974-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02974-1-znrf3-primary-antibodies-fcm-testing-3_1.jpgAnti-ZNRF3 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-ZNRF3 antibody (A02974-1). Overlay histogram showing PC-3 cells stained with A02974-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ZNRF3 Antibody (A02974-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gpcr-rdc1-cxcr-7-ackr3-picoband-trade-antibody-a02656-2-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-ackr3-primary-antibodies-wb-testing-1.jpgAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® Western blot analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR RDC1/CXCR-7/ACKR3 antigen affinity purified polyclonal antibody (Catalog # A02656-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR RDC1/CXCR-7/ACKR3 at approximately 41 kDa. The expected band size for GPCR RDC1/CXCR-7/ACKR3 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-13000_2019_780_fig2_html.pngAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband®LPS induced changes in CXCR7 expression in SGC7901 cells. A: CXCR7 protein expression was assessed by western blotting after SGC7901 cells were stimulated for different periods of time with 500 ng/mL LPS; B: SGC7901 cells were cultured with various concentrations of LPS for 24 h, and CXCR7 protein expression was analyzed via western blotting; C: After exposure of SGC7901 cells to LPS (500 ng/ml), CXCR4 protein expression was assessed by western blotting; D and E: After pretreatment with CCX771, SGC7901 cell proliferation and migration were largely inhibited in response to CXCL12 (100 ng/ml) after 48 h of incubation with LPS. *, P < 0.05, vs. the NC group. The data are presented as the mean ± SD Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13000-019-0780-x'>30636642</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-13000_2019_780_fig3_html.pngAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband®The effect of TLR4 and MD-2 knockdown on LPS-induced CXCR7 expression. a - c : Gastric cancer cells were transfected with TLR4-specific or MD-2-specific shRNAs, and endogenous TLR4 and MD-2 expression levels were analyzed via qRT-PCR ( a and b ) and western blotting ( c ); d and e : Gastric cancer cells were transfected with TLR4-specific or MD-2-specific shRNAs and treated with 500 ng/mL LPS. A CCK-8 assay was then used to detect cell proliferation ( d ), and a transwell assay was used to assess cell migration ( e ); f and g : As described in D and E, CXCR7 expression was analyzed via RT-PCR (F) and western blotting ( g ) *, P < 0.05, vs. the Lipo2000 group and sh-NC group. The data are presented as the mean ± SD Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13000-019-0780-x'>30636642</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-13000_2019_780_fig4_html.pngAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband®TLR4, MD-2 and CXCR7 expression in gastric cancer indicates poor prognosis. a : Representative immunohistochemical staining of TLR4, MD-2 and CXCR7 in gastric cancer tissues and paracancerous tissues (original magnification 400×); survival curve for patients with gastric cancer expressing TLR4 ( b ), MD-2 ( c ) and CXCR7 ( d ) Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13000-019-0780-x'>30636642</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-13000_2019_780_fig5_html.pngAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband®LPS enhances the tumorigenicity of SGC7901 cells via the TLR4/MD-2 pathway. a and b : SGC7901 cells (2 × 10 6 cells/mice) treated with different shRNAs (sh-NC, sh-TLR4 and sh-MD-2) were injected subcutaneously into the flanks of nude mice, and the mice were intratumorally injected with LPS (400 μg/kg) every other day. The tumor volume was observed ( a ) and measured ( b ); *, P < 0.05. The data are presented as the mean ± SD; C: CXCR7 expression in tumors was analyzed via immunohistochemistry (original magnification 400×) Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13000-019-0780-x'>30636642</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-ackr3-primary-antibodies-ihc-testing-2.jpgAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® IHC analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). GPCR RDC1/CXCR-7/ACKR3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPCR RDC1/CXCR-7/ACKR3 Antibody (A02656-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-ackr3-primary-antibodies-ihc-testing-3.jpgAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® IHC analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). GPCR RDC1/CXCR-7/ACKR3 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPCR RDC1/CXCR-7/ACKR3 Antibody (A02656-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-ackr3-primary-antibodies-ihc-testing-4.jpgAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® IHC analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). GPCR RDC1/CXCR-7/ACKR3 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPCR RDC1/CXCR-7/ACKR3 Antibody (A02656-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-ackr3-primary-antibodies-ihc-testing-5.jpgAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® IHC analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). GPCR RDC1/CXCR-7/ACKR3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPCR RDC1/CXCR-7/ACKR3 Antibody (A02656-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-ackr3-primary-antibodies-ihc-testing-6.jpgAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® IHC analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). GPCR RDC1/CXCR-7/ACKR3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPCR RDC1/CXCR-7/ACKR3 Antibody (A02656-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02656-2-ackr3-primary-antibodies-fcm-testing-7.jpgAnti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). Overlay histogram showing SiHa cells stained with A02656-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPCR RDC1/CXCR-7/ACKR3 Antibody (A02656-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-alpha-2a-adrenergic-receptor-adra2a-picoband-trade-antibody-a00883-3-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00883-3-adra2a-primary-antibodies-wb-testing-1_1.jpgAnti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband®Western blot analysis of ADRA2A using anti-ADRA2A antibody (A00883-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA2A antigen affinity purified polyclonal antibody (A00883-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADRA2A at approximately 51 kDa. The expected band size for ADRA2A is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00883-3-adra2a-primary-antibodies-fcm-testing-1_5.jpgAnti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband®Flow Cytometry analysis of Hela cells using anti-ADRA2A antibody (A00883-3). Overlay histogram showing Hela cells stained with A00883-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADRA2A Antibody (A00883-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cnpase-cnp-picoband-trade-antibody-a01017-4-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01017-4-cnp-primary-antibodies-wb-testing-1.jpgAnti-CNPase/CNP Antibody Picoband® Western blot analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysate, Lane 7: mouse brain tissue lysate, Lane 8: mouse Neuro-2a whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNPase/CNP antigen affinity purified polyclonal antibody (Catalog # A01017-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNPase/CNP at approximately 45 kDa. The expected band size for CNPase/CNP is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01017-4-cnp-primary-antibodies-ihc-testing-4.jpgAnti-CNPase/CNP Antibody Picoband®IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). CNPase/CNP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01017-4-cnp-primary-antibodies-ihc-testing-2.jpgAnti-CNPase/CNP Antibody Picoband® IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). CNPase/CNP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01017-4-cnp-primary-antibodies-ihc-testing-3.jpgAnti-CNPase/CNP Antibody Picoband® IHC analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). CNPase/CNP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01017-4-cnp-primary-antibodies-if-testing-4.jpgAnti-CNPase/CNP Antibody Picoband® IF analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). CNPase/CNP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01017-4-cnp-primary-antibodies-if-testing-5.jpgAnti-CNPase/CNP Antibody Picoband® IF analysis of CNPase/CNP using anti-CNPase/CNP antibody (A01017-4). CNPase/CNP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CNPase/CNP Antibody (A01017-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01017-4-cnp-primary-antibodies-fcm-testing-6.jpgAnti-CNPase/CNP Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CNPase/CNP antibody (A01017-4). Overlay histogram showing U937 cells stained with A01017-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CNPase/CNP Antibody (A01017-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dock180-dock1-picoband-trade-antibody-a03440-2-boster.html2026-03-17T05:13:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03440-2-dock1-primary-antibodies-wb-testing-1.jpgAnti-DOCK180/DOCK1 Antibody Picoband® Western blot analysis of DOCK180/DOCK1 using anti-DOCK180/DOCK1 antibody (A03440-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK180/DOCK1 antigen affinity purified polyclonal antibody (Catalog # A03440-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DOCK180/DOCK1 at approximately 215 kDa. The expected band size for DOCK180/DOCK1 is at 215 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03440-2-dock1-primary-antibodies-ihc-testing-2.jpgAnti-DOCK180/DOCK1 Antibody Picoband® IHC analysis of DOCK180/DOCK1 using anti-DOCK180/DOCK1 antibody (A03440-2). DOCK180/DOCK1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOCK180/DOCK1 Antibody (A03440-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03440-2-dock1-primary-antibodies-ihc-testing-3.jpgAnti-DOCK180/DOCK1 Antibody Picoband® IHC analysis of DOCK180/DOCK1 using anti-DOCK180/DOCK1 antibody (A03440-2). DOCK180/DOCK1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOCK180/DOCK1 Antibody (A03440-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03440-2-dock1-primary-antibodies-fcm-testing-4.jpgAnti-DOCK180/DOCK1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-DOCK180/DOCK1 antibody (A03440-2). Overlay histogram showing MCF-7 cells stained with A03440-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DOCK180/DOCK1 Antibody (A03440-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03440-2-dock1-primary-antibodies-if-testing-5.jpgAnti-DOCK180/DOCK1 Antibody Picoband® IF analysis of DOCK180/DOCK1 using anti-DOCK180/DOCK1 antibody (A03440-2). DOCK180/DOCK1 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DOCK180/DOCK1 Antibody (A03440-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03440-2-dock1-primary-antibodies-if-testing-6.jpgAnti-DOCK180/DOCK1 Antibody Picoband® IF analysis of DOCK180/DOCK1 using anti-DOCK180/DOCK1 antibody (A03440-2). DOCK180/DOCK1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DOCK180/DOCK1 Antibody (A03440-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fads1-picoband-trade-antibody-a02535-1-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02535-1-fads1-primary-antibodies-wb-testing-1.jpgAnti-FADS1 Antibody Picoband® Western blot analysis of FADS1 using anti-FADS1 antibody (A02535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS1 antigen affinity purified polyclonal antibody (Catalog # A02535-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADS1 at approximately 52 kDa. The expected band size for FADS1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02535-1-fads1-primary-antibodies-ihc-testing-2.jpgAnti-FADS1 Antibody Picoband® IHC analysis of FADS1 using anti-FADS1 antibody (A02535-1). FADS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FADS1 Antibody (A02535-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02535-1-fads1-primary-antibodies-ihc-testing-3.jpgAnti-FADS1 Antibody Picoband® IHC analysis of FADS1 using anti-FADS1 antibody (A02535-1). FADS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FADS1 Antibody (A02535-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02535-1-fads1-primary-antibodies-ihc-testing-4.jpgAnti-FADS1 Antibody Picoband® IHC analysis of FADS1 using anti-FADS1 antibody (A02535-1). FADS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FADS1 Antibody (A02535-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02535-1-fads1-primary-antibodies-fcm-testing-5.jpgAnti-FADS1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-FADS1 antibody (A02535-1). Overlay histogram showing SiHa cells stained with A02535-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FADS1 Antibody (A02535-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02535-1-fads1-primary-antibodies-if-testing-6.jpgAnti-FADS1 Antibody Picoband® IF analysis of FADS1 using anti-FADS1 antibody (A02535-1). FADS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FADS1 Antibody (A02535-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02535-1-fads1-primary-antibodies-if-testing-7.jpgAnti-FADS1 Antibody Picoband® IF analysis of FADS1 using anti-FADS1 antibody (A02535-1). FADS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FADS1 Antibody (A02535-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-kindlin-2-fermt2-picoband-trade-antibody-a03653-1-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-wb-testing-1.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® Western blot analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kindlin 2/FERMT2 antigen affinity purified polyclonal antibody (Catalog # A03653-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kindlin 2/FERMT2 at approximately 70 kDa. The expected band size for Kindlin 2/FERMT2 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-ihc-testing-2.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® IHC analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Kindlin 2/FERMT2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-ihc-testing-3.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® IHC analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Kindlin 2/FERMT2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-ihc-testing-4.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® IHC analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Kindlin 2/FERMT2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-ihc-testing-5.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® IHC analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Kindlin 2/FERMT2 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-ihc-testing-6.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® IHC analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Kindlin 2/FERMT2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-if-testing-7.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® IF analysis of Kindlin 2/FERMT2 using anti-Kindlin 2/FERMT2 antibody (A03653-1). Kindlin 2/FERMT2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-fcm-testing-8.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Kindlin 2/FERMT2 antibody (A03653-1). Overlay histogram showing SiHa cells stained with A03653-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03653-1-fermt2-primary-antibodies-fcm-testing-9.jpgAnti-Kindlin 2/FERMT2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Kindlin 2/FERMT2 antibody (A03653-1). Overlay histogram showing MCF-7 cells stained with A03653-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kindlin 2/FERMT2 Antibody (A03653-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fry-picoband-trade-antibody-a01003-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-wb-testing-1.jpgAnti-FRY Antibody Picoband® Western blot analysis of FRY using anti-FRY antibody (A01003). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat lung tissue lysate, Lane 5: mouse brain tissue lysate, Lane 6: mouse lung tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRY antigen affinity purified polyclonal antibody (Catalog # A01003) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRY at approximately 339 kDa. The expected band size for FRY is at 339 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-ihc-testing-2.jpgAnti-FRY Antibody Picoband® IHC analysis of FRY using anti-FRY antibody (A01003). FRY was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FRY Antibody (A01003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-ihc-testing-3.jpgAnti-FRY Antibody Picoband® IHC analysis of FRY using anti-FRY antibody (A01003). FRY was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FRY Antibody (A01003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-ihc-testing-4.jpgAnti-FRY Antibody Picoband® IHC analysis of FRY using anti-FRY antibody (A01003). FRY was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FRY Antibody (A01003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-ihc-testing-5.jpgAnti-FRY Antibody Picoband® IHC analysis of FRY using anti-FRY antibody (A01003). FRY was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FRY Antibody (A01003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-ihc-testing-6.jpgAnti-FRY Antibody Picoband® IHC analysis of FRY using anti-FRY antibody (A01003). FRY was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FRY Antibody (A01003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-fcm-testing-7.jpgAnti-FRY Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-FRY antibody (A01003). Overlay histogram showing SiHa cells stained with A01003 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FRY Antibody (A01003, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01003-fry-primary-antibodies-if-testing-8.jpgAnti-FRY Antibody Picoband® IF analysis of FRY using anti-FRY antibody (A01003). FRY was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FRY Antibody (A01003) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-pd1-pdcd1-picoband-trade-antibody-a00178-3-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-3-pdcd1-primary-antibodies-wb-testing-1.jpgAnti-PD1/Pdcd1 Antibody Picoband® Western blot analysis of PD1/Pdcd1 using anti-PD1/Pdcd1 antibody (A00178-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse EL-4 whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/Pdcd1 antigen affinity purified polyclonal antibody (Catalog # A00178-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD1/Pdcd1 at approximately 55 kDa. The expected band size for PD1/Pdcd1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-3-pdcd1-primary-antibodies-ihc-testing-2.jpgAnti-PD1/Pdcd1 Antibody Picoband® IHC analysis of PD1/Pdcd1 using anti-PD1/Pdcd1 antibody (A00178-3). PD1/Pdcd1 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PD1/Pdcd1 Antibody (A00178-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00178-3-pdcd1-primary-antibodies-ihc-testing-3.jpgAnti-PD1/Pdcd1 Antibody Picoband® IHC analysis of PD1/Pdcd1 using anti-PD1/Pdcd1 antibody (A00178-3). PD1/Pdcd1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PD1/Pdcd1 Antibody (A00178-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-hsd-4-rnf138-picoband-trade-antibody-a09061-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09061-rnf138-primary-antibodies-wb-testing-1.jpgAnti-HSD-4/RNF138 Antibody Picoband® Western blot analysis of HSD-4/RNF138 using anti-HSD-4/RNF138 antibody (A09061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD-4/RNF138 antigen affinity purified polyclonal antibody (Catalog # A09061) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD-4/RNF138 at approximately 28 kDa. The expected band size for HSD-4/RNF138 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09061-rnf138-primary-antibodies-fcm-testing-2.jpgAnti-HSD-4/RNF138 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-HSD-4/RNF138 antibody (A09061). Overlay histogram showing U20S cells stained with A09061 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD-4/RNF138 Antibody (A09061, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09061-rnf138-primary-antibodies-fcm-testing-3.jpgAnti-HSD-4/RNF138 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-HSD-4/RNF138 antibody (A09061). Overlay histogram showing U87 cells stained with A09061 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD-4/RNF138 Antibody (A09061, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rock1-picoband-trade-antibody-a00722-4-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00722-4-rock1-primary-antibodies-wb-testing-1.jpgAnti-ROCK1 Antibody Picoband® Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified polyclonal antibody (Catalog # A00722-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 160 kDa. The expected band size for ROCK1 is at 158 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00722-4-rock1-primary-antibodies-fcm-testing-2.jpgAnti-ROCK1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-ROCK1 antibody (A00722-4). Overlay histogram showing U20S cells stained with A00722-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROCK1 Antibody (A00722-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-s100a14-picoband-trade-antibody-a06301-1-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06301-1-s100a14-primary-antibodies-ihc-testing-1.jpgAnti-S100A14 Antibody IHC analysis of S100A14 using anti-S100A14 antibody (A06301-1). S100A14 was detected in a paraffin-embedded section of human cervica squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A14 Antibody (A06301-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06301-1-s100a14-primary-antibodies-ihc-testing-2.jpgAnti-S100A14 Antibody IHC analysis of S100A14 using anti-S100A14 antibody (A06301-1). S100A14 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-S100A14 Antibody (A06301-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-sap30-picoband-trade-antibody-a06526-1-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06526-1-sap30-primary-antibodies-wb-testing-1.jpgAnti-SAP30 Antibody Picoband® Western blot analysis of SAP30 using anti-SAP30 antibody (A06526-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP30 antigen affinity purified polyclonal antibody (Catalog # A06526-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP30 at approximately 28 kDa. The expected band size for SAP30 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06526-1-sap30-primary-antibodies-fcm-testing-2.jpgAnti-SAP30 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SAP30 antibody (A06526-1). Overlay histogram showing SiHa cells stained with A06526-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAP30 Antibody (A06526-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sirt5-picoband-trade-antibody-a02395-3-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02395-3-sirt5-primary-antibodies-wb-testing-1.jpgAnti-SIRT5 Antibody Picoband® Western blot analysis of SIRT5 using anti-SIRT5 antibody (A02395-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT5 antigen affinity purified polyclonal antibody (Catalog # A02395-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT5 at approximately 34 kDa. The expected band size for SIRT5 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02395-3-sirt5-primary-antibodies-ihc-testing-2.jpgAnti-SIRT5 Antibody Picoband® IHC analysis of SIRT5 using anti-SIRT5 antibody (A02395-3). SIRT5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIRT5 Antibody (A02395-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02395-3-sirt5-primary-antibodies-ihc-testing-3.jpgAnti-SIRT5 Antibody Picoband® IHC analysis of SIRT5 using anti-SIRT5 antibody (A02395-3). SIRT5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIRT5 Antibody (A02395-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02395-3-sirt5-primary-antibodies-fcm-testing-4.jpgAnti-SIRT5 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SIRT5 antibody (A02395-3). Overlay histogram showing HL-60 cells stained with A02395-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIRT5 Antibody (A02395-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02395-3-sirt5-primary-antibodies-fcm-testing-5.jpgAnti-SIRT5 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SIRT5 antibody (A02395-3). Overlay histogram showing Jurkat cells stained with A02395-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIRT5 Antibody (A02395-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc3a1-picoband-trade-antibody-a02654-2-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02654-2-slc3a1-primary-antibodies-wb-testing-1.jpgAnti-SLC3A1 Antibody Picoband® Western blot analysis of SLC3A1 using anti-SLC3A1 antibody (A02654-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse HBZY whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC3A1 antigen affinity purified polyclonal antibody (Catalog # A02654-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC3A1 at approximately 110 kDa. The expected band size for SLC3A1 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02654-2-slc3a1-primary-antibodies-fcm-testing-2.jpgAnti-SLC3A1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SLC3A1 antibody (A02654-2). Overlay histogram showing U20S cells stained with A02654-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC3A1 Antibody (A02654-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-gamma-synuclein-sncg-picoband-trade-antibody-a03523-4-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03523-4-sncg-primary-antibodies-wb-testing-1.jpgAnti-gamma Synuclein/SNCG Antibody Picoband® Western blot analysis of gamma Synuclein/SNCG using anti-gamma Synuclein/SNCG antibody (A03523-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Synuclein/SNCG antigen affinity purified polyclonal antibody (Catalog # A03523-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Synuclein/SNCG at approximately 17 kDa. The expected band size for gamma Synuclein/SNCG is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03523-4-sncg-primary-antibodies-fcm-testing-2.jpgAnti-gamma Synuclein/SNCG Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-gamma Synuclein/SNCG antibody (A03523-4). Overlay histogram showing Hela cells stained with A03523-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-gamma Synuclein/SNCG Antibody (A03523-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tal1-picoband-trade-antibody-a00944-4-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00944-4-tal1-primary-antibodies-wb-testing-1.jpgAnti-TAL1 Antibody Picoband® Western blot analysis of TAL1 using anti-TAL1 antibody (A00944-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat lung tissue lysate, Lane 5: rat spleen tissue lysate, Lane 6: mouse lung tissue lysate, Lane 7: mouse spleen tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAL1 antigen affinity purified polyclonal antibody (Catalog # A00944-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAL1 at approximately 40-45 kDa. The expected band size for TAL1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00944-4-tal1-primary-antibodies-fcm-testing-2.jpgAnti-TAL1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TAL1 antibody (A00944-4). Overlay histogram showing HEL cells stained with A00944-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAL1 Antibody (A00944-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tead3-picoband-trade-antibody-a07748-2-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07748-2-tead3-primary-antibodies-wb-testing-1.jpgAnti-TEAD3 Antibody Picoband® Western blot analysis of TEAD3 using anti-TEAD3 antibody (A07748-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD3 antigen affinity purified polyclonal antibody (Catalog # A07748-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEAD3 at approximately 54 kDa. The expected band size for TEAD3 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tim-2-timd2-picoband-trade-antibody-a32807-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32807-timd2-primary-antibodies-wb-testing-1.jpgAnti-Tim 2/Timd2 Antibody Picoband® Western blot analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody (A32807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tim 2/Timd2 antigen affinity purified polyclonal antibody (Catalog # A32807) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tim 2/Timd2 at approximately 70 kDa. The expected band size for Tim 2/Timd2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32807-timd2-primary-antibodies-ihc-testing-2.jpgAnti-Tim 2/Timd2 Antibody Picoband® IHC analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody (A32807). Tim 2/Timd2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tim 2/Timd2 Antibody (A32807) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32807-timd2-primary-antibodies-ihc-testing-3.jpgAnti-Tim 2/Timd2 Antibody Picoband® IHC analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody (A32807). Tim 2/Timd2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tim 2/Timd2 Antibody (A32807) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32807-timd2-primary-antibodies-fcm-testing-4.jpgAnti-Tim 2/Timd2 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Tim 2/Timd2 antibody (A32807). Overlay histogram showing HEPA1-6 cells stained with A32807 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Tim 2/Timd2 Antibody (A32807, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32807-timd2-primary-antibodies-if-testing-5.jpgAnti-Tim 2/Timd2 Antibody Picoband® IF analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody (A32807). Tim 2/Timd2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Tim 2/Timd2 Antibody (A32807) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tkr-tktl1-picoband-trade-antibody-a05309-1-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05309-1-tktl1-primary-antibodies-wb-testing-1.jpgAnti-TKR/TKTL1 Antibody Picoband® Western blot analysis of TKR/TKTL1 using anti-TKR/TKTL1 antibody (A05309-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TKR/TKTL1 antigen affinity purified polyclonal antibody (Catalog # A05309-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TKR/TKTL1 at approximately 65 kDa. The expected band size for TKR/TKTL1 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tm7sf3-picoband-trade-antibody-a15928-1-boster.html2026-03-17T05:13:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15928-1-tm7sf3-primary-antibodies-wb-testing-1.jpgAnti-TM7SF3 Antibody Picoband® Western blot analysis of TM7SF3 using anti-TM7SF3 antibody (A15928-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM7SF3 antigen affinity purified polyclonal antibody (Catalog # A15928-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM7SF3 at approximately 85 kDa. The expected band size for TM7SF3 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15928-1-tm7sf3-primary-antibodies-fcm-testing-2.jpgAnti-TM7SF3 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TM7SF3 antibody (A15928-1). Overlay histogram showing SiHa cells stained with A15928-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TM7SF3 Antibody (A15928-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmc7-picoband-trade-antibody-a14820-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14820-1-tmc7-primary-antibodies-wb-testing-1.jpgAnti-TMC7 Antibody Picoband® Western blot analysis of TMC7 using anti-TMC7 antibody (A14820-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMC7 antigen affinity purified polyclonal antibody (Catalog # A14820-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMC7 at approximately 90 kDa. The expected band size for TMC7 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14820-1-tmc7-primary-antibodies-ihc-testing-2.jpgAnti-TMC7 Antibody Picoband® IHC analysis of TMC7 using anti-TMC7 antibody (A14820-1). TMC7 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMC7 Antibody (A14820-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14820-1-tmc7-primary-antibodies-ihc-testing-3.jpgAnti-TMC7 Antibody Picoband® IHC analysis of TMC7 using anti-TMC7 antibody (A14820-1). TMC7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMC7 Antibody (A14820-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14820-1-tmc7-primary-antibodies-fcm-testing-4.jpgAnti-TMC7 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TMC7 antibody (A14820-1). Overlay histogram showing SiHa cells stained with A14820-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMC7 Antibody (A14820-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14820-1-tmc7-primary-antibodies-if-testing-5.jpgAnti-TMC7 Antibody Picoband® IF analysis of TMC7 using anti-TMC7 antibody (A14820-1). TMC7 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TMC7 Antibody (A14820-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tmem52b-picoband-trade-antibody-a17417-1-boster.html2026-04-06T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17417-1-tmem52b-primary-antibodies-wb-testing-1.jpgAnti-TMEM52B Antibody Picoband® Western blot analysis of TMEM52B using anti-TMEM52B antibody (A17417-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse HBZY-1 whole cell lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM52B antigen affinity purified polyclonal antibody (Catalog # A17417-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM52B at approximately 24 kDa. The expected band size for TMEM52B is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17417-1-tmem52b-primary-antibodies-fcm-testing-2_1.jpgAnti-TMEM52B Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-TMEM52B antibody (A17417-1). Overlay histogram showing RT4 cells stained with A17417-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM52B Antibody (A17417-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmem65-picoband-trade-antibody-a13650-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13650-1-tmem65-primary-antibodies-wb-testing-1.jpgAnti-TMEM65 Antibody Picoband® Western blot analysis of TMEM65 using anti-TMEM65 antibody (A13650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM65 antigen affinity purified polyclonal antibody (Catalog # A13650-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM65 at approximately 20 kDa. The expected band size for TMEM65 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13650-1-tmem65-primary-antibodies-fcm-testing-2.jpgAnti-TMEM65 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TMEM65 antibody (A13650-1). Overlay histogram showing SiHa cells stained with A13650-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM65 Antibody (A13650-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13650-1-tmem65-primary-antibodies-fcm-testing-3.jpgAnti-TMEM65 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-TMEM65 antibody (A13650-1). Overlay histogram showing ANA-1 cells stained with A13650-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM65 Antibody (A13650-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13650-1-tmem65-primary-antibodies-fcm-testing-4.jpgAnti-TMEM65 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-TMEM65 antibody (A13650-1). Overlay histogram showing RH35 cells stained with A13650-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM65 Antibody (A13650-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmem88-picoband-trade-antibody-a12951-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12951-1-tmem88-primary-antibodies-wb-testing-1.jpgAnti-TMEM88 Antibody Picoband® Western blot analysis of TMEM88 using anti-TMEM88 antibody (A12951-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM88 antigen affinity purified polyclonal antibody (Catalog # A12951-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM88 at approximately 22 kDa. The expected band size for TMEM88 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12951-1-tmem88-primary-antibodies-if-testing-2.jpgAnti-TMEM88 Antibody Picoband® IF analysis of TMEM88 using anti-TMEM88 antibody (A12951-1). TMEM88 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMEM88 Antibody (A12951-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tmem129-picoband-trade-antibody-a11755-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11755-tmem129-primary-antibodies-wb-testing-1.jpgAnti-TMEM129 Antibody Picoband® Western blot analysis of TMEM129 using anti-TMEM129 antibody (A11755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM129 antigen affinity purified polyclonal antibody (Catalog # A11755) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM129 at approximately 50 kDa. The expected band size for TMEM129 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11755-tmem129-primary-antibodies-fcm-testing-2.jpgAnti-TMEM129 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TMEM129 antibody (A11755). Overlay histogram showing SiHa cells stained with A11755 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM129 Antibody (A11755, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmprss13-picoband-trade-antibody-a10970-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10970-1-tmprss13-primary-antibodies-wb-testing-1.jpgAnti-TMPRSS13 Antibody Picoband® Western blot analysis of TMPRSS13 using anti-TMPRSS13 antibody (A10970-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS13 antigen affinity purified polyclonal antibody (Catalog # A10970-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS13 at approximately 63 kDa. The expected band size for TMPRSS13 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10970-1-tmprss13-primary-antibodies-ihc-testing-2.jpgAnti-TMPRSS13 Antibody Picoband® IHC analysis of TMPRSS13 using anti-TMPRSS13 antibody (A10970-1). TMPRSS13 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMPRSS13 Antibody (A10970-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10970-1-tmprss13-primary-antibodies-ihc-testing-3.jpgAnti-TMPRSS13 Antibody Picoband® IHC analysis of TMPRSS13 using anti-TMPRSS13 antibody (A10970-1). TMPRSS13 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMPRSS13 Antibody (A10970-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10970-1-tmprss13-primary-antibodies-ihc-testing-4.jpgAnti-TMPRSS13 Antibody Picoband® IHC analysis of TMPRSS13 using anti-TMPRSS13 antibody (A10970-1). TMPRSS13 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMPRSS13 Antibody (A10970-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10970-1-tmprss13-primary-antibodies-fcm-testing-5.jpgAnti-TMPRSS13 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TMPRSS13 antibody (A10970-1). Overlay histogram showing MCF-7 cells stained with A10970-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMPRSS13 Antibody (A10970-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10970-1-tmprss13-primary-antibodies-if-testing-6.jpgAnti-TMPRSS13 Antibody Picoband® IF analysis of TMPRSS13 using anti-TMPRSS13 antibody (A10970-1). TMPRSS13 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TMPRSS13 Antibody (A10970-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10970-1-tmprss13-primary-antibodies-if-testing-7.jpgAnti-TMPRSS13 Antibody Picoband® IF analysis of TMPRSS13 using anti-TMPRSS13 antibody (A10970-1). TMPRSS13 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TMPRSS13 Antibody (A10970-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-4-1bbl-tnfsf9-picoband-trade-antibody-a06032-2-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06032-2-tnfsf9-primary-antibodies-wb-testing-1.jpgAnti-4-1BBL/Tnfsf9 Antibody Picoband® Western blot analysis of 4-1BBL/Tnfsf9 using anti-4-1BBL/Tnfsf9 antibody (A06032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-4-1BBL/Tnfsf9 antigen affinity purified polyclonal antibody (Catalog # A06032-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 4-1BBL/Tnfsf9 at approximately 27 kDa. The expected band size for 4-1BBL/Tnfsf9 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06032-2-tnfsf9-primary-antibodies-fcm-testing-2.jpgAnti-4-1BBL/Tnfsf9 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-4-1BBL/Tnfsf9 antibody (A06032-2). Overlay histogram showing ANA-1 cells stained with A06032-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-4-1BBL/Tnfsf9 Antibody (A06032-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tox2-picoband-trade-antibody-a12956-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12956-1-tox2-primary-antibodies-wb-testing-1.jpgAnti-TOX2 Antibody Picoband® Western blot analysis of TOX2 using anti-TOX2 antibody (A12956-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX2 antigen affinity purified polyclonal antibody (Catalog # A12956-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOX2 at approximately 70 kDa. The expected band size for TOX2 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12956-1-tox2-primary-antibodies-if-testing-2.jpgAnti-TOX2 Antibody Picoband® IF analysis of TOX2 using anti-TOX2 antibody (A12956-1). TOX2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOX2 Antibody (A12956-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12956-1-tox2-primary-antibodies-fcm-testing-3.jpgAnti-TOX2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TOX2 antibody (A12956-1). Overlay histogram showing MCF-7 cells stained with A12956-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOX2 Antibody (A12956-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tropomyosin-2-tpm2-picoband-trade-antibody-a03082-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-wb-testing-1.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® Western blot analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat H9C2(2-1) whole cell lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tropomyosin 2/TPM2 antigen affinity purified polyclonal antibody (Catalog # A03082-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tropomyosin 2/TPM2 at approximately 38 kDa. The expected band size for Tropomyosin 2/TPM2 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-ihc-testing-2.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® IHC analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Tropomyosin 2/TPM2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-ihc-testing-3.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® IHC analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Tropomyosin 2/TPM2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-ihc-testing-4.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® IHC analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Tropomyosin 2/TPM2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-ihc-testing-5.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® IHC analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Tropomyosin 2/TPM2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-fcm-testing-6.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Overlay histogram showing SiHa cells stained with A03082-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-fcm-testing-7.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Overlay histogram showing ANA-1 cells stained with A03082-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-fcm-testing-8.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Overlay histogram showing RH35 cells stained with A03082-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03082-1-tpm2-primary-antibodies-if-testing-9.jpgAnti-Tropomyosin 2/TPM2 Antibody Picoband® IF analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Tropomyosin 2/TPM2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Tropomyosin 2/TPM2 Antibody (A03082-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tripeptidyl-peptidase-ii-tppii-tpp2-picoband-trade-antibody-a06668-2-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06668-2-tpp2-primary-antibodies-wb-testing-1.jpgAnti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® Western blot analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 antigen affinity purified polyclonal antibody (Catalog # A06668-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tripeptidyl peptidase II/TPPII/TPP2 at approximately 138 kDa. The expected band size for Tripeptidyl peptidase II/TPPII/TPP2 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06668-2-tpp2-primary-antibodies-wb-testing-2.jpgAnti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® Western blot analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 antigen affinity purified polyclonal antibody (Catalog # A06668-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tripeptidyl peptidase II/TPPII/TPP2 at approximately 138 kDa. The expected band size for Tripeptidyl peptidase II/TPPII/TPP2 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06668-2-tpp2-primary-antibodies-ihc-testing-3.jpgAnti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® IHC analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2). Tripeptidyl peptidase II/TPPII/TPP2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06668-2-tpp2-primary-antibodies-ihc-testing-4.jpgAnti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® IHC analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2). Tripeptidyl peptidase II/TPPII/TPP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06668-2-tpp2-primary-antibodies-ihc-testing-5.jpgAnti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® IHC analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2). Tripeptidyl peptidase II/TPPII/TPP2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06668-2-tpp2-primary-antibodies-if-testing-6.jpgAnti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® IF analysis of Tripeptidyl peptidase II/TPPII/TPP2 using anti-Tripeptidyl peptidase II/TPPII/TPP2 antibody (A06668-2). Tripeptidyl peptidase II/TPPII/TPP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody (A06668-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06668-2-tpp2-primary-antibodies-fcm-testing-7.jpgAnti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Tropomyosin 2/TPM2 antibody (A06668-2). Overlay histogram showing SiHa cells stained with A06668-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (A06668-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tppp-picoband-trade-antibody-a04389-2-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04389-2-tpp2-primary-antibodies-wb-testing-1.jpgAnti-TPPP Antibody Picoband® Western blot analysis of TPPP using anti-TPPP antibody (A04389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPPP antigen affinity purified polyclonal antibody (Catalog # A04389-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPPP at approximately 24 kDa. The expected band size for TPPP is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04389-2-tppp-primary-antibodies-fcm-testing-2.jpgAnti-TPPP Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TPPP antibody (A04389-2). Overlay histogram showing HEL cells stained with A04389-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPPP Antibody (A04389-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-traf7-picoband-trade-antibody-a05972-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05972-1-traf7-primary-antibodies-wb-testing-1.jpgAnti-TRAF7 Antibody Picoband® Western blot analysis of TRAF7 using anti-TRAF7 antibody (A05972-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF7 antigen affinity purified polyclonal antibody (Catalog # A05972-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF7 at approximately 75 kDa. The expected band size for TRAF7 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05972-1-traf7-primary-antibodies-fcm-testing-2.jpgAnti-TRAF7 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TRAF7 antibody (A05972-1). Overlay histogram showing U87 cells stained with A05972-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF7 Antibody (A05972-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trip-traip-picoband-trade-antibody-a07953-2-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07953-2-traip-primary-antibodies-wb-testing-1.jpgAnti-TRIP/TRAIP Antibody Picoband® Western blot analysis of TRIP/TRAIP using anti-TRIP/TRAIP antibody (A07953-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIP/TRAIP antigen affinity purified polyclonal antibody (Catalog # A07953-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIP/TRAIP at approximately 53 kDa. The expected band size for TRIP/TRAIP is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07953-2-traip-primary-antibodies-if-testing-2.jpgAnti-TRIP/TRAIP Antibody Picoband® IF analysis of TRIP/TRAIP using anti-TRIP/TRAIP antibody (A07953-2). TRIP/TRAIP was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRIP/TRAIP Antibody (A07953-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07953-2-traip-primary-antibodies-fcm-testing-3.jpgAnti-TRIP/TRAIP Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TRIP/TRAIP antibody (A07953-2). Overlay histogram showing SiHa cells stained with A07953-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIP/TRAIP Antibody (A07953-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tram1-picoband-trade-antibody-a04106-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04106-tram1-primary-antibodies-wb-testing-1.jpgAnti-TRAM1 Antibody Picoband® Western blot analysis of TRAM1 using anti-TRAM1 antibody (A04106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAM1 antigen affinity purified polyclonal antibody (Catalog # A04106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAM1 at approximately 55-60 kDa. The expected band size for TRAM1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04106-tram1-primary-antibodies-fcm-testing-2.jpgAnti-TRAM1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TRAM1 antibody (A04106). Overlay histogram showing SiHa cells stained with A04106 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAM1 Antibody (A04106, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tram1l1-picoband-trade-antibody-a18207-1-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18207-1-tram1l1-primary-antibodies-wb-testing-1.jpgAnti-TRAM1L1 Antibody Picoband® Western blot analysis of TRAM1L1 using anti-TRAM1L1 antibody (A18207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAM1L1 antigen affinity purified polyclonal antibody (Catalog # A18207-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAM1L1 at approximately 52 kDa. The expected band size for TRAM1L1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18207-1-tram1l1-primary-antibodies-ihc-testing-2.jpgAnti-TRAM1L1 Antibody Picoband® IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody (A18207-1). TRAM1L1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRAM1L1 Antibody (A18207-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18207-1-tram1l1-primary-antibodies-ihc-testing-3.jpgAnti-TRAM1L1 Antibody Picoband® IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody (A18207-1). TRAM1L1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRAM1L1 Antibody (A18207-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18207-1-tram1l1-primary-antibodies-ihc-testing-4.jpgAnti-TRAM1L1 Antibody Picoband® IHC analysis of TRAM1L1 using anti-TRAM1L1 antibody (A18207-1). TRAM1L1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRAM1L1 Antibody (A18207-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18207-1-tram1l1-primary-antibodies-if-testing-5.jpgAnti-TRAM1L1 Antibody Picoband® IF analysis of TRAM1L1 using anti-TRAM1L1 antibody (A18207-1). TRAM1L1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRAM1L1 Antibody (A18207-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18207-1-tram1l1-primary-antibodies-fcm-testing-6.jpgAnti-TRAM1L1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TRAM1L1 antibody (A18207-1). Overlay histogram showing HEL cells stained with A18207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAM1L1 Antibody (A18207-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trank1-picoband-trade-antibody-a09518-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-wb-testing-1.jpgAnti-TRANK1 Antibody Picoband® Western blot analysis of TRANK1 using anti-TRANK1 antibody (A09518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRANK1 antigen affinity purified polyclonal antibody (Catalog # A09518) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRANK1 at approximately 336 kDa. The expected band size for TRANK1 is at 336 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-ihc-testing-2.jpgAnti-TRANK1 Antibody Picoband® IHC analysis of TRANK1 using anti-TRANK1 antibody (A09518). TRANK1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRANK1 Antibody (A09518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-ihc-testing-3.jpgAnti-TRANK1 Antibody Picoband® IHC analysis of TRANK1 using anti-TRANK1 antibody (A09518). TRANK1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRANK1 Antibody (A09518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-ihc-testing-4.jpgAnti-TRANK1 Antibody Picoband® IHC analysis of TRANK1 using anti-TRANK1 antibody (A09518). TRANK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRANK1 Antibody (A09518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-if-testing-5.jpgAnti-TRANK1 Antibody Picoband® IF analysis of TRANK1 using anti-TRANK1 antibody (A09518). TRANK1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRANK1 Antibody (A09518) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-fcm-testing-6.jpgAnti-TRANK1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti--TRANK1 antibody (A09518). Overlay histogram showing HEL cells stained with A09518 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRANK1 Antibody (A09518, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-fcm-testing-7.jpgAnti-TRANK1 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti--TRANK1 antibody (A09518). Overlay histogram showing U251 cells stained with A09518 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRANK1 Antibody (A09518, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-if-testing-8.jpgAnti-TRANK1 Antibody Picoband® IF analysis of TRANK1 using anti-TRANK1 antibody (A09518). TRANK1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRANK1 Antibody (A09518) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-if-testing-9.jpgAnti-TRANK1 Antibody Picoband® IF analysis of TRANK1 using anti-TRANK1 antibody (A09518). TRANK1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRANK1 Antibody (A09518) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09518-trank1-primary-antibodies-if-testing-10.jpgAnti-TRANK1 Antibody Picoband® IF analysis of TRANK1 using anti-TRANK1 antibody (A09518). TRANK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRANK1 Antibody (A09518) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-trat1-picoband-trade-antibody-a11223-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11223-trat1-primary-antibodies-wb-testing-1.jpgAnti-TRAT1 Antibody Picoband® Western blot analysis of TRAT1 using anti-TRAT1 antibody (A11223). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAT1 antigen affinity purified polyclonal antibody (Catalog # A11223) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAT1 at approximately 27-30 kDa. The expected band size for TRAT1 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11223-trat1-primary-antibodies-fcm-testing-2.jpgAnti-TRAT1 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-TRAT1 antibody (A11223). Overlay histogram showing U251 cells stained with A11223 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAT1 Antibody (A11223, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-triadin-trdn-picoband-trade-antibody-a06901-boster.html2026-03-17T05:13:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06901-trdn-primary-antibodies-wb-testing-1.jpgAnti-Triadin/TRDN Antibody Picoband® Western blot analysis of Triadin/TRDN using anti-Triadin/TRDN antibody (A06901). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Triadin/TRDN antigen affinity purified polyclonal antibody (Catalog # A06901) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Triadin/TRDN at approximately 25 kDa. The expected band size for Triadin/TRDN is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06901-trdn-primary-antibodies-fcm-testing-2.jpgAnti-Triadin/TRDN Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Triadin/TRDN antibody (A06901). Overlay histogram showing MCF-7 cells stained with A06901 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Triadin/TRDN Antibody (A06901, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-treml2-picoband-trade-antibody-a08894-1-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08894-1-treml2-primary-antibodies-wb-testing-1.jpgAnti-TREML2 Antibody Picoband® Western blot analysis of TREML2 using anti-TREML2 antibody (A08894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREML2 antigen affinity purified polyclonal antibody (Catalog # A08894-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREML2 at approximately 70 kDa. The expected band size for TREML2 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08894-1-treml2-primary-antibodies-fcm-testing-2.jpgAnti-TREML2 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TREML2 antibody (A08894-1). Overlay histogram showing HEL cells stained with A08894-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TREML2 Antibody (A08894-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trim3-picoband-trade-antibody-a07081-2-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07081-2-trim3-primary-antibodies-wb-testing-1.jpgAnti-TRIM3 Antibody Picoband® Western blot analysis of TRIM3 using anti-TRIM3 antibody (A07081-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM3 antigen affinity purified polyclonal antibody (Catalog # A07081-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM3 at approximately 81 kDa. The expected band size for TRIM3 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07081-2-trim3-primary-antibodies-fcm-testing-2_1.jpgAnti-TRIM3 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TRIM3 antibody (A07081-2). Overlay histogram showing 293T cells stained with A07081-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM3 Antibody (A07081-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trim29-picoband-trade-antibody-a04472-1-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04472-1-trim29-primary-antibodies-wb-testing-1.jpgAnti-TRIM29 Antibody Picoband® Western blot analysis of TRIM29 using anti-TRIM29 antibody (A04472-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM29 antigen affinity purified polyclonal antibody (Catalog # A04472-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM29 at approximately 70 kDa. The expected band size for TRIM29 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04472-1-trim29-primary-antibodies-fcm-testing-2.jpgAnti-TRIM29 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-TRIM29 antibody (A04472-1). Overlay histogram showing RT4 cells stained with A04472-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM29 Antibody (A04472-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trim50-picoband-trade-antibody-a14090-1-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14090-1-trim50-primary-antibodies-wb-testing-1.jpgAnti-TRIM50 Antibody Picoband® Western blot analysis of TRIM50 using anti-TRIM50 antibody (A14090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM50 antigen affinity purified polyclonal antibody (Catalog # A14090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM50 at approximately 55 kDa. The expected band size for TRIM50 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-trim67-picoband-trade-antibody-a16666-1-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16666-1-trim67-primary-antibodies-wb-testing-1.jpgAnti-TRIM67 Antibody Picoband® Western blot analysis of TRIM67 using anti-TRIM67 antibody (A16666-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM67 antigen affinity purified polyclonal antibody (Catalog # A16666-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM67 at approximately 72 kDa. The expected band size for TRIM67 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16666-1-trim67-primary-antibodies-fcm-testing-2.jpgAnti-TRIM67 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TRIM67 antibody (A16666-1). Overlay histogram showing HEL cells stained with A16666-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM67 Antibody (A16666-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mg53-trim72-picoband-trade-antibody-a06982-2-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06982-2-trim72-primary-antibodies-wb-testing-1.jpgAnti-MG53/TRIM72 Antibody Picoband® Western blot analysis of MG53/TRIM72 using anti-MG53/TRIM72 antibody (A06982-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MG53/TRIM72 antigen affinity purified polyclonal antibody (Catalog # A06982-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MG53/TRIM72 at approximately 55 kDa. The expected band size for MG53/TRIM72 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06982-2-trim72-primary-antibodies-ihc-testing-2.jpgAnti-MG53/TRIM72 Antibody Picoband® IHC analysis of MG53/TRIM72 using anti-MG53/TRIM72 antibody (A06982-2). MG53/TRIM72 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MG53/TRIM72 Antibody (A06982-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06982-2-trim72-primary-antibodies-ihc-testing-3.jpgAnti-MG53/TRIM72 Antibody Picoband® IHC analysis of MG53/TRIM72 using anti-MG53/TRIM72 antibody (A06982-2). MG53/TRIM72 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MG53/TRIM72 Antibody (A06982-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06982-2-trim72-primary-antibodies-ihc-testing-4.jpgAnti-MG53/TRIM72 Antibody Picoband® IHC analysis of MG53/TRIM72 using anti-MG53/TRIM72 antibody (A06982-2). MG53/TRIM72 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MG53/TRIM72 Antibody (A06982-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06982-2-trim72-primary-antibodies-if-testing-5.jpgAnti-MG53/TRIM72 Antibody Picoband® IF analysis of MG53/TRIM72 using anti-MG53/TRIM72 antibody (A06982-2). MG53/TRIM72 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MG53/TRIM72 Antibody (A06982-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06982-2-trim72-primary-antibodies-fcm-testing-6.jpgAnti-MG53/TRIM72 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-MG53/TRIM72 antibody (A06982-2). Overlay histogram showing THP-1 cells stained with A06982-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MG53/TRIM72 Antibody (A06982-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pch2-trip13-picoband-trade-antibody-a06921-2-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06921-2-trip13-primary-antibodies-wb-testing-1.jpgAnti-PCH2/TRIP13 Antibody Picoband® Western blot analysis of PCH2/TRIP13 using anti-PCH2/TRIP13 antibody (A06921-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCH2/TRIP13 antigen affinity purified polyclonal antibody (Catalog # A06921-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCH2/TRIP13 at approximately 49 kDa. The expected band size for PCH2/TRIP13 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06921-2-trip13-primary-antibodies-fcm-testing-2.jpgAnti-PCH2/TRIP13 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PCH2/TRIP13 antibody (A06921-2). Overlay histogram showing 293T cells stained with A06921-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCH2/TRIP13 Antibody (A06921-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trir-picoband-trade-antibody-a32362-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32362-trir-primary-antibodies-wb-testing-1.jpgAnti-TRIR Antibody Picoband® Western blot analysis of TRIR using anti-TRIR antibody (A32362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIR antigen affinity purified polyclonal antibody (Catalog # A32362) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIR at approximately 24 kDa. The expected band size for TRIR is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32362-trir-primary-antibodies-ihc-testing-2.jpgAnti-TRIR Antibody Picoband® IHC analysis of TRIR using anti-TRIR antibody (A32362). TRIR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIR Antibody (A32362) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32362-trir-primary-antibodies-ihc-testing-3.jpgAnti-TRIR Antibody Picoband® IHC analysis of TRIR using anti-TRIR antibody (A32362). TRIR was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIR Antibody (A32362) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32362-trir-primary-antibodies-ihc-testing-4.jpgAnti-TRIR Antibody Picoband® IHC analysis of TRIR using anti-TRIR antibody (A32362). TRIR was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIR Antibody (A32362) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32362-trir-primary-antibodies-ihc-testing-5.jpgAnti-TRIR Antibody Picoband® IHC analysis of TRIR using anti-TRIR antibody (A32362). TRIR was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIR Antibody (A32362) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ipt-trit1-picoband-trade-antibody-a08098-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08098-trit1-primary-antibodies-wb-testing-1.jpgAnti-IPT/TRIT1 Antibody Picoband® Western blot analysis of IPT/TRIT1 using anti-IPT/TRIT1 antibody (A08098). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPT/TRIT1 antigen affinity purified polyclonal antibody (Catalog # A08098) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IPT/TRIT1 at approximately 53 kDa. The expected band size for IPT/TRIT1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08098-trit1-primary-antibodies-fcm-testing-2.jpgAnti-IPT/TRIT1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-IPT/TRIT1 antibody (A08098). Overlay histogram showing 293T cells stained with A08098 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IPT/TRIT1 Antibody (A08098, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trmt6-picoband-trade-antibody-a13158-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-wb-testing-1.jpgAnti-TRMT6 Antibody Picoband® Western blot analysis of TRMT6 using anti-TRMT6 antibody (A13158). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT6 antigen affinity purified polyclonal antibody (Catalog # A13158) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT6 at approximately 60 kDa. The expected band size for TRMT6 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-ihc-testing-2.jpgAnti-TRMT6 Antibody Picoband® IHC analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human breast cnacer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-ihc-testing-3.jpgAnti-TRMT6 Antibody Picoband® IHC analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-ihc-testing-4.jpgAnti-TRMT6 Antibody Picoband® IHC analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-ihc-testing-5.jpgAnti-TRMT6 Antibody Picoband® IHC analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-ihc-testing-6.jpgAnti-TRMT6 Antibody Picoband® IHC analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-ihc-testing-7.jpgAnti-TRMT6 Antibody Picoband® IHC analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-ihc-testing-8.jpgAnti-TRMT6 Antibody Picoband® IHC analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of hunan lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-if-testing-9.jpgAnti-TRMT6 Antibody Picoband® IF analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-fcm-testing-10.jpgAnti-TRMT6 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TRMT6 antibody (A13158). Overlay histogram showing 293T cells stained with A13158 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT6 Antibody (A13158, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-if-testing-11.jpgAnti-TRMT6 Antibody Picoband® IF analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-if-testing-12.jpgAnti-TRMT6 Antibody Picoband® IF analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13158-trmt6-primary-antibodies-if-testing-13.jpgAnti-TRMT6 Antibody Picoband® IF analysis of TRMT6 using anti-TRMT6 antibody (A13158). TRMT6 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT6 Antibody (A13158) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-trmt10c-picoband-trade-antibody-a09612-1-boster.html2026-03-17T05:13:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-wb-testing-1.jpgAnti-TRMT10C Antibody Picoband® Western blot analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT10C antigen affinity purified polyclonal antibody (Catalog # A09612-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT10C at approximately 40-45 kDa. The expected band size for TRMT10C is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-ihc-testing-2.jpgAnti-TRMT10C Antibody Picoband® IHC analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human breast duct carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-ihc-testing-3.jpgAnti-TRMT10C Antibody Picoband® IHC analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-ihc-testing-4.jpgAnti-TRMT10C Antibody Picoband® IHC analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-ihc-testing-5.jpgAnti-TRMT10C Antibody Picoband® IHC analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-ihc-testing-6.jpgAnti-TRMT10C Antibody Picoband® IHC analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-ihc-testing-7.jpgAnti-TRMT10C Antibody Picoband® IHC analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-ihc-testing-8.jpgAnti-TRMT10C Antibody Picoband® IHC analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-if-testing-9.jpgAnti-TRMT10C Antibody Picoband® IF analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-if-testing-10.jpgAnti-TRMT10C Antibody Picoband® IF analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-if-testing-11.jpgAnti-TRMT10C Antibody Picoband® IF analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). TRMT10C was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT10C Antibody (A09612-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09612-1-trmt10c-primary-antibodies-fcm-testing-12.jpgAnti-TRMT10C Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TRMT10C antibody (A09612-1). Overlay histogram showing 293T cells stained with A09612-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT10C Antibody (A09612-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trmt44-picoband-trade-antibody-a14732-1-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14732-1-trmt44-primary-antibodies-wb-testing-1.jpgAnti-TRMT44 Antibody Picoband® Western blot analysis of TRMT44 using anti-TRMT44 antibody (A14732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT44 antigen affinity purified polyclonal antibody (Catalog # A14732-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT44 at approximately 85 kDa. The expected band size for TRMT44 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14732-1-trmt44-primary-antibodies-fcm-testing-2.jpgAnti-TRMT44 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TRMT44 antibody (A14732-1). Overlay histogram showing 293T cells stained with A14732-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT44 Antibody (A14732-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trmt61b-picoband-trade-antibody-a12682-2-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12682-2-trmt61b-primary-antibodies-wb-testing-1.jpgAnti-TRMT61B Antibody Picoband® Western blot analysis of TRMT61B using anti-TRMT61B antibody (A12682-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT61B antigen affinity purified polyclonal antibody (Catalog # A12682-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT61B at approximately 57 kDa. The expected band size for TRMT61B is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12682-2-trmt61b-primary-antibodies-ihc-testing-2.jpgAnti-TRMT61B Antibody Picoband® IHC analysis of TRMT61B using anti-TRMT61B antibody (A12682-2). TRMT61B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61B Antibody (A12682-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12682-2-trmt61b-primary-antibodies-ihc-testing-3.jpgAnti-TRMT61B Antibody Picoband® IHC analysis of TRMT61B using anti-TRMT61B antibody (A12682-2). TRMT61B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61B Antibody (A12682-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12682-2-trmt61b-primary-antibodies-ihc-testing-4.jpgAnti-TRMT61B Antibody Picoband® IHC analysis of TRMT61B using anti-TRMT61B antibody (A12682-2). TRMT61B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61B Antibody (A12682-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12682-2-trmt61b-primary-antibodies-ihc-testing-5.jpgAnti-TRMT61B Antibody Picoband® IHC analysis of TRMT61B using anti-TRMT61B antibody (A12682-2). TRMT61B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61B Antibody (A12682-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12682-2-trmt61b-primary-antibodies-fcm-testing-6.jpgAnti-TRMT61B Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TRMT61B antibody (A12682-2). Overlay histogram showing 293T cells stained with A12682-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT61B Antibody (A12682-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12682-2-trmt61b-primary-antibodies-if-testing-7.jpgAnti-TRMT61B Antibody Picoband® IF analysis of TRMT61B using anti-TRMT61B antibody (A12682-2). TRMT61B was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT61B Antibody (A12682-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tspyl5-picoband-trade-antibody-a10648-2-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10648-2-tspyl5-primary-antibodies-wb-testing-1.jpgAnti-TSPYL5 Antibody Picoband® Western blot analysis of TSPYL5 using anti-TSPYL5 antibody (A10648-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPYL5 antigen affinity purified polyclonal antibody (Catalog # A10648-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSPYL5 at approximately 48 kDa. The expected band size for TSPYL5 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10648-2-tspyl5-primary-antibodies-fcm-testing-2.jpgAnti-TSPYL5 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TSPYL5 antibody (A10648-2). Overlay histogram showing MCF-7 cells stained with A10648-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSPYL5 Antibody (A10648-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-twist1-2-picoband-trade-antibody-a00980-1-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-wb-testing-1.jpgAnti-TWIST1/2 Antibody Picoband® Western blot analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat ovary tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse ovary tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWIST1/2 antigen affinity purified polyclonal antibody (Catalog # A00980-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWIST1/2 at approximately 30 kDa. The expected band size for TWIST1/2 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-ihc-testing-2.jpgAnti-TWIST1/2 Antibody Picoband® IHC analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). TWIST1/2 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1/2 Antibody (A00980-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-ihc-testing-3.jpgAnti-TWIST1/2 Antibody Picoband® IHC analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). TWIST1/2 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1/2 Antibody (A00980-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-ihc-testing-4.jpgAnti-TWIST1/2 Antibody Picoband® IHC analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). TWIST1/2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1/2 Antibody (A00980-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-ihc-testing-5.jpgAnti-TWIST1/2 Antibody Picoband® IHC analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). TWIST1/2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1/2 Antibody (A00980-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-ihc-testing-6.jpgAnti-TWIST1/2 Antibody Picoband® IHC analysis of TWIST1/2 using anti-TWIST1/2 antibody (A00980-1). TWIST1/2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1/2 Antibody (A00980-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-fcm-testing-7_1.jpgAnti-TWIST1/2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TWIST1/2 antibody (A00980-1). Overlay histogram showing THP-1 cells stained with A00980-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TWIST1/2 Antibody (A00980-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-fcm-testing-8.jpgAnti-TWIST1/2 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-TWIST1/2 antibody (A00980-1). Overlay histogram showing RAW264.7 cells stained with A00980-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TWIST1/2 Antibody (A00980-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00980-1-twist1-2-primary-antibodies-fcm-testing-9.jpgAnti-TWIST1/2 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-TWIST1/2 antibody (A00980-1). Overlay histogram showing RH35 cells stained with A00980-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TWIST1/2 Antibody (A00980-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-vapa-picoband-trade-antibody-a05329-1-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-wb-testing-1.jpgAnti-VAPA Antibody Picoband® Western blot analysis of VAPA using anti-VAPA antibody (A05329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAPA antigen affinity purified polyclonal antibody (Catalog # A05329-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAPA at approximately 33 kDa. The expected band size for VAPA is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-ihc-testing-2.jpgAnti-VAPA Antibody Picoband® IHC analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-ihc-testing-3.jpgAnti-VAPA Antibody Picoband® IHC analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-ihc-testing-4.jpgAnti-VAPA Antibody Picoband® IHC analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-ihc-testing-5.jpgAnti-VAPA Antibody Picoband® IHC analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-ihc-testing-6.jpgAnti-VAPA Antibody Picoband® IHC analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-ihc-testing-7.jpgAnti-VAPA Antibody Picoband® IHC analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-if-testing-8.jpgAnti-VAPA Antibody Picoband® IF analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-if-testing-9.jpgAnti-VAPA Antibody Picoband® IF analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-if-testing-10.jpgAnti-VAPA Antibody Picoband® IF analysis of VAPA using anti-VAPA antibody (A05329-1). VAPA was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-VAPA Antibody (A05329-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05329-1-vapa-primary-antibodies-fcm-testing-11.jpgAnti-VAPA Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-VAPA antibody (A05329-1). Overlay histogram showing U87 cells stained with A05329-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VAPA Antibody (A05329-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-xrcc4-picoband-trade-antibody-a00787-2-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-wb-testing-1.jpgAnti-XRCC4 Antibody Picoband® Western blot analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC4 antigen affinity purified polyclonal antibody (Catalog # A00787-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC4 at approximately 55 kDa. The expected band size for XRCC4 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-ihc-testing-2.jpgAnti-XRCC4 Antibody Picoband® IHC analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-ihc-testing-3.jpgAnti-XRCC4 Antibody Picoband® IHC analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in a paraffin-embedded section of human breast duct carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-ihc-testing-4.jpgAnti-XRCC4 Antibody Picoband® IHC analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-ihc-testing-5.jpgAnti-XRCC4 Antibody Picoband® IHC analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-ihc-testing-6.jpgAnti-XRCC4 Antibody Picoband® IHC analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-ihc-testing-7.jpgAnti-XRCC4 Antibody Picoband® IHC analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-if-testing-8.jpgAnti-XRCC4 Antibody Picoband® IF analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-if-testing-9.jpgAnti-XRCC4 Antibody Picoband® IF analysis of XRCC4 using anti-XRCC4 antibody (A00787-2). XRCC4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-XRCC4 Antibody (A00787-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00787-2-xrcc4-primary-antibodies-fcm-testing-10.jpgAnti-XRCC4 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-XRCC4 antibody (A00787-2). Overlay histogram showing 293T cells stained with A00787-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XRCC4 Antibody (A00787-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wdr44-picoband-trade-antibody-a11988-1-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-wb-testing-1.jpgAnti-WDR44 Antibody Picoband® Western blot analysis of WDR44 using anti-WDR44 antibody (A11988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR44 antigen affinity purified polyclonal antibody (Catalog # A11988-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR44 at approximately 130 kDa. The expected band size for WDR44 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-ihc-testing-2.jpgAnti-WDR44 Antibody Picoband® IHC analysis of WDR44 using anti-WDR44 antibody (A11988-1). WDR44 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR44 Antibody (A11988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-ihc-testing-3.jpgAnti-WDR44 Antibody Picoband® IHC analysis of WDR44 using anti-WDR44 antibody (A11988-1). WDR44 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR44 Antibody (A11988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-ihc-testing-4.jpgAnti-WDR44 Antibody Picoband® IHC analysis of WDR44 using anti-WDR44 antibody (A11988-1). WDR44 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR44 Antibody (A11988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-ihc-testing-5.jpgAnti-WDR44 Antibody Picoband® IHC analysis of WDR44 using anti-WDR44 antibody (A11988-1). WDR44 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR44 Antibody (A11988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-ihc-testing-6.jpgAnti-WDR44 Antibody Picoband® IHC analysis of WDR44 using anti-WDR44 antibody (A11988-1). WDR44 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR44 Antibody (A11988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-ihc-testing-7.jpgAnti-WDR44 Antibody Picoband® IHC analysis of WDR44 using anti-WDR44 antibody (A11988-1). WDR44 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR44 Antibody (A11988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11988-1-wdr44-primary-antibodies-fcm-testing-8.jpgAnti-WDR44 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-WDR44 antibody (A11988-1). Overlay histogram showing SiHa cells stained with A11988-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR44 Antibody (A11988-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-zbtb48-picoband-trade-antibody-a13220-2-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13220-2-zbtb48-primary-antibodies-wb-testing-1.jpgAnti-ZBTB48 Antibody Picoband® Western blot analysis of ZBTB48 using anti-ZBTB48 antibody (A13220-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB48 antigen affinity purified polyclonal antibody (Catalog # A13220-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB48 at approximately 77 kDa. The expected band size for ZBTB48 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13220-2-zbtb48-primary-antibodies-fcm-testing-2.jpgAnti-ZBTB48 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-ZBTB48 antibody (A13220-2). Overlay histogram showing 293T cells stained with A13220-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZBTB48 Antibody (A13220-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-acad9-picoband-trade-antibody-a05223-2-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-2-acad9-primary-antibodies-wb-testing-1.jpgAnti-ACAD9 Antibody Picoband® Western blot analysis of ACAD9 using anti-ACAD9 antibody (A05223-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAD9 antigen affinity purified polyclonal antibody (Catalog # A05223-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAD9 at approximately 65 kDa. The expected band size for ACAD9 is at 69 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sah-acsm3-picoband-trade-antibody-a08748-3-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08748-3-acsm3-primary-antibodies-wb-testing-1.jpgAnti-SAH/ACSM3 Antibody Picoband® Western blot analysis of SAH/ACSM3 using anti-SAH/ACSM3 antibody (A08748-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAH/ACSM3 antigen affinity purified polyclonal antibody (Catalog # A08748-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAH/ACSM3 at approximately 66 kDa. The expected band size for SAH/ACSM3 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08748-3-acsm3-primary-antibodies-fcm-testing-2.pngAnti-SAH/ACSM3 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SAH/ACSM3 antibody (A08748-3). Overlay histogram showing HEL cells stained with A08748-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAH/ACSM3 Antibody (A08748-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08748-3-acsm3-primary-antibodies-fcm-testing-3.pngAnti-SAH/ACSM3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SAH/ACSM3 antibody (A08748-3). Overlay histogram showing HepG2 cells stained with A08748-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAH/ACSM3 Antibody (A08748-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atg4c-picoband-trade-antibody-a09728-2-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09728-2-atg4c-primary-antibodies-wb-testing-1.jpgAnti-ATG4C Antibody Picoband® Western blot analysis of ATG4C using anti-ATG4C antibody (A09728-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4C antigen affinity purified polyclonal antibody (Catalog # A09728-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4C at approximately 52 kDa. The expected band size for ATG4C is at 52 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09728-2-atg4c-primary-antibodies-if-testing-2.jpgAnti-ATG4C Antibody Picoband® IF analysis of ATG4C using anti-ATG4C antibody (A09728-2) and anti-Tubulin Alpha antibody (M03989-3). ATG4C was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG4C Antibody (A09728-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09728-2-atg4c-primary-antibodies-fcm-testing-3.pngAnti-ATG4C Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-ATG4C antibody (A09728-2). Overlay histogram showing U251 cells stained with A09728-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4C Antibody (A09728-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd74-picoband-trade-antibody-a01340-4-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01340-4-cd74-primary-antibodies-wb-testing-1.jpgAnti-CD74 Antibody Picoband® Western blot analysis of CD74 using anti-CD74 antibody (A01340-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD74 antigen affinity purified polyclonal antibody (Catalog # A01340-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD74 at approximately 31-34 kDa. The expected band size for CD74 is at 34 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01340-4-cd74-primary-antibodies-ihc-testing-2.jpgAnti-CD74 Antibody Picoband® IHC analysis of CD74 using anti-CD74 antibody (A01340-4). CD74 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD74 Antibody (A01340-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01340-4-cd74-primary-antibodies-ihc-testing-3.jpgAnti-CD74 Antibody Picoband® IHC analysis of CD74 using anti-CD74 antibody (A01340-4). CD74 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD74 Antibody (A01340-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01340-4-cd74-primary-antibodies-if-testing-4.jpgAnti-CD74 Antibody Picoband® IF analysis of CD74 using anti-CD74 antibody (A01340-4). CD74 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD74 Antibody (A01340-4) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01340-4-cd74-primary-antibodies-fcm-testing-5.pngAnti-CD74 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-CD74 antibody (A01340-4). Overlay histogram showing Daudi cells stained with A01340-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD74 Antibody (A01340-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dectin-1-clec7a-picoband-trade-antibody-a02731-2-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02731-2-clec7a-primary-antibodies-wb-testing-1.jpgAnti-Dectin-1/CLEC7A Antibody Picoband® Western blot analysis of Dectin-1/CLEC7A using anti-Dectin-1/CLEC7A antibody (A02731-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dectin-1/CLEC7A antigen affinity purified polyclonal antibody (Catalog # A02731-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dectin-1/CLEC7A at approximately 36 kDa. The expected band size for Dectin-1/CLEC7A is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02731-2-clec7a-primary-antibodies-fcm-testing-2.pngAnti-Dectin-1/CLEC7A Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-Dectin-1/CLEC7A antibody (A02731-2). Overlay histogram showing U937 cells stained with A02731-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Dectin-1/CLEC7A Antibody (A02731-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hsd11b2-picoband-trade-antibody-a01613-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-wb-testing-1.jpgAnti-HSD11B2 Antibody Picoband® Western blot analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B2 antigen affinity purified polyclonal antibody (Catalog # A01613) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B2 at approximately 40 kDa. The expected band size for HSD11B2 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-ihc-testing-2.jpgAnti-HSD11B2 Antibody Picoband® IHC analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). HSD11B2 was detected in a paraffin-embedded section of human appendix cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (A01613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-ihc-testing-3.jpgAnti-HSD11B2 Antibody Picoband® IHC analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). HSD11B2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (A01613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-ihc-testing-4.jpgAnti-HSD11B2 Antibody Picoband® IHC analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). HSD11B2 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (A01613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-ihc-testing-5.jpgAnti-HSD11B2 Antibody Picoband® IHC analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). HSD11B2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (A01613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-ihc-testing-6.jpgAnti-HSD11B2 Antibody Picoband® IHC analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). HSD11B2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (A01613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-ihc-testing-7.jpgAnti-HSD11B2 Antibody Picoband® IHC analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). HSD11B2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (A01613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-if-testing-8.jpgAnti-HSD11B2 Antibody Picoband® IF analysis of HSD11B2 using anti-HSD11B2 antibody (A01613). HSD11B2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HSD11B2 Antibody (A01613) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01613-hsd11b2-primary-antibodies-fcm-testing-9.pngAnti-HSD11B2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-HSD11B2 antibody (A01613). Overlay histogram showing HL-60 cells stained with A01613 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD11B2 Antibody (A01613, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-inf2-picoband-trade-antibody-a02710-1-boster.html2026-03-17T05:13:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-wb-testing-1_1.jpgAnti-INF2 Antibody Picoband® Western blot analysis of INF2 using anti-INF2 antibody (A02710-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INF2 antigen affinity purified polyclonal antibody (Catalog # A02710-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INF2 at approximately 180 kDa. The expected band size for INF2 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-ihc-testing-1.jpgAnti-INF2 Antibody Picoband®IHC analysis of INF2 using anti-INF2 antibody (A02710-1). INF2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INF2 Antibody (A02710-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-ihc-testing-2.jpgAnti-INF2 Antibody Picoband®IHC analysis of INF2 using anti-INF2 antibody (A02710-1). INF2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INF2 Antibody (A02710-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-ihc-testing-3.jpgAnti-INF2 Antibody Picoband®IHC analysis of INF2 using anti-INF2 antibody (A02710-1). INF2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INF2 Antibody (A02710-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-ihc-testing-4.jpgAnti-INF2 Antibody Picoband®IHC analysis of INF2 using anti-INF2 antibody (A02710-1). INF2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INF2 Antibody (A02710-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-if-testing-2.jpgAnti-INF2 Antibody Picoband® IF analysis of INF2 using anti-INF2 antibody (A02710-1). INF2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-INF2 Antibody (A02710-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-if-testing-3.jpgAnti-INF2 Antibody Picoband®IF analysis of INF2 using anti-INF2 antibody (A02710-1). INF2 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-INF2 Antibody (A02710-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-if-testing-4.jpgAnti-INF2 Antibody Picoband®IF analysis of INF2 using anti-INF2 antibody (A02710-1). INF2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-INF2 Antibody (A02710-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-ip-testing-4.jpgAnti-INF2 Antibody Picoband® Immunoprecipitating INF2 in Hela whole cell lysate. Western blot analysis of INF2 using anti-INF2 antibody (A02710-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-INF2 antibody in Hela whole cell lysate; Lane 3: anti-INF2 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-INF2 antigen affinity purified polyclonal antibody (A02710-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for INF2 at approximately 180 kDa. The expected band size for INF2 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02710-1-inf2-primary-antibodies-fcm-testing-3_1.pngAnti-INF2 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-INF2 antibody (A02710-1). Overlay histogram showing HEL cells stained with A02710-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-INF2 Antibody (A02710-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-inpp4a-picoband-trade-antibody-a07116-1-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07116-1-inpp4a-primary-antibodies-wb-testing-1.jpgAnti-INPP4A Antibody Picoband® Western blot analysis of INPP4A using anti-INPP4A antibody (A07116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP4A antigen affinity purified polyclonal antibody (Catalog # A07116-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP4A at approximately 110 kDa. The expected band size for INPP4A is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07116-1-inpp4a-primary-antibodies-if-testing-2.jpgAnti-INPP4A Antibody Picoband® IF analysis of INPP4A using anti-INPP4A antibody (A07116-1). INPP4A was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-INPP4A Antibody (A07116-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-itk-picoband-trade-antibody-a01385-3-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01385-3-itk-primary-antibodies-wb-testing-1.jpgAnti-ITK Antibody Picoband® Western blot analysis of ITK using anti-ITK antibody (A01385-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITK antigen affinity purified polyclonal antibody (Catalog # A01385-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITK at approximately 72 kDa. The expected band size for ITK is at 72 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01385-3-itk-primary-antibodies-ihc-testing-2.jpgAnti-ITK Antibody Picoband® IHC analysis of ITK using anti-ITK antibody (A01385-3). ITK was detected in a paraffin-embedded section of human spleen cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITK Antibody (A01385-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01385-3-itk-primary-antibodies-fcm-testing-3.pngAnti-ITK Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-ITK antibody (A01385-3). Overlay histogram showing HEL cells stained with A01385-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITK Antibody (A01385-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nob1-picoband-trade-antibody-a03504-1-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03504-1-nob1-primary-antibodies-wb-testing-1.jpgAnti-NOB1 Antibody Picoband® Western blot analysis of NOB1 using anti-NOB1 antibody (A03504-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOB1 antigen affinity purified polyclonal antibody (Catalog # A03504-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOB1 at approximately 47 kDa. The expected band size for NOB1 is at 47 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03504-1-nob1-primary-antibodies-ihc-testing-4.jpgAnti-NOB1 Antibody Picoband®IHC analysis of NOB1 using anti-NOB1 antibody (A03504-1). NOB1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOB1 Antibody (A03504-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03504-1-nob1-primary-antibodies-ihc-testing-2.jpgAnti-NOB1 Antibody Picoband® IHC analysis of NOB1 using anti-NOB1 antibody (A03504-1). NOB1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOB1 Antibody (A03504-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03504-1-nob1-primary-antibodies-ihc-testing-3.jpgAnti-NOB1 Antibody Picoband® IHC analysis of NOB1 using anti-NOB1 antibody (A03504-1). NOB1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOB1 Antibody (A03504-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-zac-plagl1-picoband-trade-antibody-a03220-2-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03220-2-plagl1-primary-antibodies-wb-testing-1.jpgAnti-ZAC/Plagl1 Antibody Picoband® Western blot analysis of ZAC/Plagl1 using anti-ZAC/Plagl1 antibody (A03220-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZAC/Plagl1 antigen affinity purified polyclonal antibody (Catalog # A03220-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZAC/Plagl1 at approximately 51 kDa. The expected band size for ZAC/Plagl1 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03220-2-plagl1-primary-antibodies-if-testing-2.jpgAnti-ZAC/Plagl1 Antibody Picoband® IF analysis of ZAC/Plagl1 using anti-ZAC/Plagl1 antibody (A03220-2). ZAC/Plagl1 was detected in an immunocytochemical section of RM1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZAC/Plagl1 Antibody (A03220-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03220-2-plagl1-primary-antibodies-fcm-testing-3.pngAnti-ZAC/Plagl1 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-ZAC/Plagl1 antibody (A03220-2). Overlay histogram showing ANA-1 cells stained with A03220-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZAC/Plagl1 Antibody (A03220-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sae1-picoband-trade-antibody-a04753-3-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-wb-testing-1.jpgAnti-SAE1 Antibody Picoband® Western blot analysis of SAE1 using anti-SAE1 antibody (A04753-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE1 antigen affinity purified polyclonal antibody (Catalog # A04753-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE1 at approximately 38 kDa. The expected band size for SAE1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-ihc-testing-2.jpgAnti-SAE1 Antibody Picoband® IHC analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of human laryngeal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-ihc-testing-3.jpgAnti-SAE1 Antibody Picoband® IHC analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of human duodenum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-ihc-testing-4.jpgAnti-SAE1 Antibody Picoband® IHC analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-ihc-testing-5.jpgAnti-SAE1 Antibody Picoband® IHC analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-ihc-testing-6.jpgAnti-SAE1 Antibody Picoband® IHC analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-ihc-testing-7.jpgAnti-SAE1 Antibody Picoband® IHC analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-ihc-testing-8.jpgAnti-SAE1 Antibody Picoband® IHC analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-if-testing-9.jpgAnti-SAE1 Antibody Picoband® IF analysis of SAE1 using anti-SAE1 antibody (A04753-3) and anti-Tubulin Alpha antibody (M03989-3). SAE1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SAE1 Antibody (A04753-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-if-testing-10.jpgAnti-SAE1 Antibody Picoband® IF analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-if-testing-11.jpgAnti-SAE1 Antibody Picoband® IF analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04753-3-sae1-primary-antibodies-if-testing-12.jpgAnti-SAE1 Antibody Picoband® IF analysis of SAE1 using anti-SAE1 antibody (A04753-3). SAE1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SAE1 Antibody (A04753-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-sage1-picoband-trade-antibody-a15250-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15250-sag1-primary-antibodies-wb-testing-1_1.jpgAnti-SAGE1 Antibody Picoband® Western blot analysis of SAGE1 using anti-SAGE1 antibody (A15250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAGE1 antigen affinity purified polyclonal antibody (Catalog # A15250) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAGE1 at approximately 125 kDa. The expected band size for SAGE1 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15250-sag1-primary-antibodies-ihc-testing-2_1.jpgAnti-SAGE1 Antibody Picoband® IHC analysis of SAGE1 using anti-SAGE1 antibody (A15250). SAGE1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAGE1 Antibody (A15250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15250-sag1-primary-antibodies-if-testing-3_1.jpgAnti-SAGE1 Antibody Picoband® IF analysis of SAGE1 using anti-SAGE1 antibody (A15250) and anti-Beta Tubulin antibody (M01857-3). SAGE1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SAGE1 Antibody (A15250) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15250-sag1-primary-antibodies-fcm-testing-4.jpgAnti-SAGE1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SAGE1 antibody (A15250). Overlay histogram showing K562 cells stained with A15250 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAGE1 Antibody (A15250, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-samsn1-picoband-trade-antibody-a08977-2-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08977-2-samsn1-primary-antibodies-wb-testing-1_1.jpgAnti-SAMSN1 Antibody Picoband® Western blot analysis of SAMSN1 using anti-SAMSN1 antibody (A08977-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAMSN1 antigen affinity purified polyclonal antibody (Catalog # A08977-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAMSN1 at approximately 50 kDa. The expected band size for SAMSN1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08977-2-samsn1-primary-antibodies-ihc-testing-2.jpgAnti-SAMSN1 Antibody Picoband® IHC analysis of SAMSN1 using anti-SAMSN1 antibody (A08977-2). SAMSN1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAMSN1 Antibody (A08977-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08977-2-samsn1-primary-antibodies-ihc-testing-3.jpgAnti-SAMSN1 Antibody Picoband® IHC analysis of SAMSN1 using anti-SAMSN1 antibody (A08977-2). SAMSN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAMSN1 Antibody (A08977-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08977-2-samsn1-primary-antibodies-ihc-testing-4.jpgAnti-SAMSN1 Antibody Picoband® IHC analysis of SAMSN1 using anti-SAMSN1 antibody (A08977-2). SAMSN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAMSN1 Antibody (A08977-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08977-2-samsn1-primary-antibodies-if-testing-5.jpgAnti-SAMSN1 Antibody Picoband® IF analysis of SAMSN1 using anti-SAMSN1 antibody (A08977-2). SAMSN1 was detected in an immunocytochemical section of HEL cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SAMSN1 Antibody (A08977-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08977-2-samsn1-primary-antibodies-fcm-testing-6.jpgAnti-SAMSN1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SAMSN1 antibody (A08977-2). Overlay histogram showing K562 cells stained with A08977-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAMSN1 Antibody (A08977-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sap18-picoband-trade-antibody-a06381-1-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-wb-testing-1.jpgAnti-SAP18 Antibody Picoband® Western blot analysis of SAP18 using anti-SAP18 antibody (A06381-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIN/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP18 antigen affinity purified polyclonal antibody (Catalog # A06381-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP18 at approximately 18 kDa. The expected band size for SAP18 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-if-testing-2.jpgAnti-SAP18 Antibody Picoband® IF analysis of SAP18 using anti-SAP18 antibody (A06381-1) and anti-Tubulin Alpha antibody (M03989-3). SAP18 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SAP18 Antibody (A06381-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-if-testing-3.jpgAnti-SAP18 Antibody Picoband® IF analysis of SAP18 using anti-SAP18 antibody (A06381-1). SAP18 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SAP18 Antibody (A06381-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-if-testing-4.jpgAnti-SAP18 Antibody Picoband® IF analysis of SAP18 using anti-SAP18 antibody (A06381-1). SAP18 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SAP18 Antibody (A06381-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-ihc-testing-5.jpgAnti-SAP18 Antibody Picoband® IHC analysis of SAP18 using anti-SAP18 antibody (A06381-1). SAP18 was detected in a paraffin-embedded section of human duodenal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP18 Antibody (A06381-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-ihc-testing-6.jpgAnti-SAP18 Antibody Picoband® IHC analysis of SAP18 using anti-SAP18 antibody (A06381-1). SAP18 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP18 Antibody (A06381-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-ihc-testing-7.jpgAnti-SAP18 Antibody Picoband® IHC analysis of SAP18 using anti-SAP18 antibody (A06381-1). SAP18 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP18 Antibody (A06381-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06381-1-sap18-primary-antibodies-fcm-testing-8.pngAnti-SAP18 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SAP18 antibody (A06381-1). Overlay histogram showing U20S cells stained with A06381-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAP18 Antibody (A06381-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sap130-picoband-trade-antibody-a07578-1-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-wb-testing-1.jpgAnti-SAP130 Antibody Picoband® Western blot analysis of SAP130 using anti-SAP130 antibody (A07578-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP130 antigen affinity purified polyclonal antibody (Catalog # A07578-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP130 at approximately 130 kDa. The expected band size for SAP130 is at 110 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-2.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-3.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of human ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-4.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-5.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-6.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-7.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-8.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-ihc-testing-9.jpgAnti-SAP130 Antibody Picoband® IHC analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-if-testing-10.jpgAnti-SAP130 Antibody Picoband® IF analysis of SAP130 using anti-SAP130 antibody (A07578-1) and anti-Tubulin Alpha antibody (M03989-3). SAP130 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SAP130 Antibody (A07578-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-if-testing-11.jpgAnti-SAP130 Antibody Picoband® IF analysis of SAP130 using anti-SAP130 antibody (A07578-1). SAP130 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SAP130 Antibody (A07578-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07578-1-sap130-primary-antibodies-fcm-testing-12.pngAnti-SAP130 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SAP130 antibody (A07578-1). Overlay histogram showing U20S cells stained with A07578-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAP130 Antibody (A07578-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sardh-picoband-trade-antibody-a09641-1-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09641-1-sardh-primary-antibodies-wb-testing-1.jpgAnti-SARDH Antibody Picoband® Western blot analysis of SARDH using anti-SARDH antibody (A09641-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: ran pancreas tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARDH antigen affinity purified polyclonal antibody (Catalog # A09641-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SARDH at approximately 101 kDa. The expected band size for SARDH is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09641-1-sardh-primary-antibodies-fcm-testing-2.pngAnti-SARDH Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SARDH antibody (A09641-1). Overlay histogram showing U87 cells stained with A09641-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SARDH Antibody (A09641-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-seryl-trna-synthetase-sers-sars1-picoband-trade-antibody-a00501-1-boster.html2026-03-17T05:13:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00501-1-sars1-primary-antibodies-wb-testing-1.jpgAnti-Seryl-tRNA synthetase/SERS/SARS1 Antibody Picoband® Western blot analysis of Seryl-TRNA Synthetase/SERS/SARS1 using anti-Seryl-TRNA Synthetase/SERS/SARS1 antibody (A00501-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Seryl-TRNA Synthetase/SERS/SARS1 antigen affinity purified polyclonal antibody (Catalog # A00501-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Seryl-TRNA Synthetase/SERS/SARS1 at approximately 59 kDa. The expected band size for Seryl-TRNA Synthetase/SERS/SARS1 is at 59 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00501-1-sars1-primary-antibodies-if-testing-2.jpgAnti-Seryl-tRNA synthetase/SERS/SARS1 Antibody Picoband® IF analysis of Seryl-TRNA Synthetase/SERS/SARS1 using anti-Seryl-TRNA Synthetase/SERS/SARS1 antibody (A00501-1). Seryl-TRNA Synthetase/SERS/SARS1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Seryl-TRNA Synthetase/SERS/SARS1 Antibody (A00501-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00501-1-sars1-primary-antibodies-fcm-testing-3.pngAnti-Seryl-tRNA synthetase/SERS/SARS1 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-Seryl-TRNA Synthetase/SERS/SARS1 antibody (A00501-1). Overlay histogram showing Caco-2 cells stained with A00501-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Seryl-TRNA Synthetase/SERS/SARS1 Antibody (A00501-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00501-1-sars1-primary-antibodies-fcm-testing-4.pngAnti-Seryl-tRNA synthetase/SERS/SARS1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-Seryl-TRNA Synthetase/SERS/SARS1 antibody (A00501-1). Overlay histogram showing HL-60 cells stained with A00501-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Seryl-TRNA Synthetase/SERS/SARS1 Antibody (A00501-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sars2-picoband-trade-antibody-a08425-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-wb-testing-1.jpgAnti-SARS2 Antibody Picoband® Western blot analysis of SARS2 using anti-SARS2 antibody (A08425-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARS2 antigen affinity purified polyclonal antibody (Catalog # A08425-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SARS2 at approximately 53 kDa. The expected band size for SARS2 is at 58 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-ihc-testing-2.jpgAnti-SARS2 Antibody Picoband® IHC analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-ihc-testing-3.jpgAnti-SARS2 Antibody Picoband® IHC analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-ihc-testing-4.jpgAnti-SARS2 Antibody Picoband® IHC analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-ihc-testing-5.jpgAnti-SARS2 Antibody Picoband® IHC analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-ihc-testing-6.jpgAnti-SARS2 Antibody Picoband® IHC analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-ihc-testing-7.jpgAnti-SARS2 Antibody Picoband® IHC analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-ihc-testing-8.jpgAnti-SARS2 Antibody Picoband® IHC analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-if-testing-9.jpgAnti-SARS2 Antibody Picoband® IF analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08425-1-sars2-primary-antibodies-if-testing-10.jpgAnti-SARS2 Antibody Picoband® IF analysis of SARS2 using anti-SARS2 antibody (A08425-1). SARS2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SARS2 Antibody (A08425-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-sart1-picoband-trade-antibody-a05414-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-wb-testing-1.jpgAnti-SART1 Antibody Picoband® Western blot analysis of SART1 using anti-SART1 antibody (A05414-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SART1 antigen affinity purified polyclonal antibody (Catalog # A05414-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SART1 at approximately 110 kDa. The expected band size for SART1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-ihc-testing-2.jpgAnti-SART1 Antibody Picoband® IHC analysis of SART1 using anti-SART1 antibody (A05414-1). SART1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART1 Antibody (A05414-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-ihc-testing-3.jpgAnti-SART1 Antibody Picoband® IHC analysis of SART1 using anti-SART1 antibody (A05414-1). SART1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART1 Antibody (A05414-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-ihc-testing-4.jpgAnti-SART1 Antibody Picoband® IHC analysis of SART1 using anti-SART1 antibody (A05414-1). SART1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART1 Antibody (A05414-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-ihc-testing-5.jpgAnti-SART1 Antibody Picoband® IHC analysis of SART1 using anti-SART1 antibody (A05414-1). SART1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART1 Antibody (A05414-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-ihc-testing-6.jpgAnti-SART1 Antibody Picoband® IHC analysis of SART1 using anti-SART1 antibody (A05414-1). SART1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART1 Antibody (A05414-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-if-testing-7.jpgAnti-SART1 Antibody Picoband® IF analysis of SART1 using anti-SART1 antibody (A05414-1) and anti-Beta Tubulin antibody (M01857-3). SART1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SART1 Antibody (A05414-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-if-testing-8.jpgAnti-SART1 Antibody Picoband® IF analysis of SART1 using anti-SART1 antibody (A05414-1). SART1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SART1 Antibody (A05414-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05414-1-sart1-primary-antibodies-fcm-testing-9.pngAnti-SART1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SART1 antibody (A05414-1). Overlay histogram showing U20S cells stained with A05414-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SART1 Antibody (A05414-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sart3-picoband-trade-antibody-a03973-2-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03973-2-sart3-primary-antibodies-wb-testing-1.jpgAnti-SART3 Antibody Picoband® Western blot analysis of SART3 using anti-SART3 antibody (A03973-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SART3 antigen affinity purified polyclonal antibody (Catalog # A03973-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SART3 at approximately 130 kDa. The expected band size for SART3 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03973-2-sart3-primary-antibodies-ihc-testing-2.jpgAnti-SART3 Antibody Picoband® IHC analysis of SART3 using anti-SART3 antibody (A03973-2). SART3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART3 Antibody (A03973-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03973-2-sart3-primary-antibodies-ihc-testing-3.jpgAnti-SART3 Antibody Picoband® IHC analysis of SART3 using anti-SART3 antibody (A03973-2). SART3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART3 Antibody (A03973-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03973-2-sart3-primary-antibodies-ihc-testing-4.jpgAnti-SART3 Antibody Picoband® IHC analysis of SART3 using anti-SART3 antibody (A03973-2). SART3 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SART3 Antibody (A03973-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03973-2-sart3-primary-antibodies-if-testing-5_1.jpgAnti-SART3 Antibody Picoband® IF analysis of SART3 using anti-SART3 antibody (A03973-2). SART3 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SART3 Antibody (A03973-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03973-2-sart3-primary-antibodies-if-testing-6.jpgAnti-SART3 Antibody Picoband® IF analysis of SART3 using anti-SART3 antibody (A03973-2) and anti-Tubulin Alpha antibody (M03989-3). SART3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SART3 Antibody (A03973-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-sav1-picoband-trade-antibody-a04183-2-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04183-2-sav1-primary-antibodies-wb-testing-1.jpgAnti-SAV1 Antibody Picoband® Western blot analysis of SAV1 using anti-SAV1 antibody (A04183-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human DLD-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAV1 antigen affinity purified polyclonal antibody (Catalog # A04183-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAV1 at approximately 45 kDa. The expected band size for SAV1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04183-2-sav1-primary-antibodies-ihc-testing-2.jpgAnti-SAV1 Antibody Picoband® IHC analysis of SAV1 using anti-SAV1 antibody (A04183-2). SAV1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAV1 Antibody (A04183-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04183-2-sav1-primary-antibodies-ihc-testing-3.jpgAnti-SAV1 Antibody Picoband® IHC analysis of SAV1 using anti-SAV1 antibody (A04183-2). SAV1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAV1 Antibody (A04183-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04183-2-sav1-primary-antibodies-ihc-testing-4.jpgAnti-SAV1 Antibody Picoband® IHC analysis of SAV1 using anti-SAV1 antibody (A04183-2). SAV1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAV1 Antibody (A04183-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04183-2-sav1-primary-antibodies-fcm-testing-5.pngAnti-SAV1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SAV1 antibody (A04183-2). Overlay histogram showing U87 cells stained with A04183-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAV1 Antibody (A04183-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sbds-picoband-trade-antibody-a02396-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02396-1-sbds-primary-antibodies-wb-testing-1_1.jpgAnti-SBDS Antibody Picoband® Western blot analysis of SBDS using anti-SBDS antibody (A02396-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SBDS antigen affinity purified polyclonal antibody (Catalog # A02396-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SBDS at approximately 29-32 kDa. The expected band size for SBDS is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02396-1-sbds-primary-antibodies-if-testing-2.jpgAnti-SBDS Antibody Picoband® IF analysis of SBDS using anti-SBDS antibody (A02396-1) and anti-Beta Tubulin antibody (M01857-3). SBDS was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SBDS Antibody (A02396-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02396-1-sbds-primary-antibodies-fcm-testing-3.jpgAnti-SBDS Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SBDS antibody (A02396-1). Overlay histogram showing HEL cells stained with A02396-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SBDS Antibody (A02396-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-scamp1-picoband-trade-antibody-a08692-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08692-1-scamp1-primary-antibodies-wb-testing-1.jpgAnti-SCAMP1 Antibody Picoband® Western blot analysis of SCAMP1 using anti-SCAMP1 antibody (A08692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human T98G whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse pancreas tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCAMP1 antigen affinity purified polyclonal antibody (Catalog # A08692-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCAMP1 at approximately 35 kDa. The expected band size for SCAMP1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08692-1-scamp1-primary-antibodies-fcm-testing-2.pngAnti-SCAMP1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SCAMP1 antibody (A08692-1). Overlay histogram showing U87 cells stained with A08692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCAMP1 Antibody (A08692-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-scara3-picoband-trade-antibody-a11048-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11048-1-scara3-primary-antibodies-wb-testing-1.jpgAnti-SCARA3 Antibody Picoband® Western blot analysis of SCARA3 using anti-SCARA3 antibody (A11048-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARA3 antigen affinity purified polyclonal antibody (Catalog # A11048-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARA3 at approximately 65 kDa. The expected band size for SCARA3 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-scavenging-receptor-srb2-scarb2-picoband-trade-antibody-a05090-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-wb-testing-1.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® Western blot analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SRB2/SCARB2 antigen affinity purified polyclonal antibody (Catalog # A05090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scavenging Receptor SRB2/SCARB2 at approximately 80 kDa. The expected band size for Scavenging Receptor SRB2/SCARB2 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-ihc-testing-2.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (A05090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-ihc-testing-7.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband®IHC analysis of LIMPII/SCARB2 using anti-LIMPII/SCARB2 antibody (A05090-1). LIMPII/SCARB2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIMPII/SCARB2 Antibody (A05090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-ihc-testing-3.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (A05090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-ihc-testing-4.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (A05090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-ihc-testing-5.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (A05090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-ihc-testing-6.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® IHC analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (A05090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-if-testing-7.jpgAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® IF analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Scavenging Receptor SRB2/SCARB2 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (A05090-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05090-1-scarb2-primary-antibodies-fcm-testing-8.pngAnti-Scavenging Receptor SRB2/SCARB2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Overlay histogram showing U87 cells stained with A05090-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Scavenging Receptor SRB2/SCARB2 Antibody (A05090-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-scin-picoband-trade-antibody-a07463-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07463-scin-primary-antibodies-wb-testing-1.jpgAnti-SCIN Antibody Picoband® Western blot analysis of SCIN using anti-SCIN antibody (A07463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCIN antigen affinity purified polyclonal antibody (Catalog # A07463) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCIN at approximately 80 kDa. The expected band size for SCIN is at 80 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-scml1-picoband-trade-antibody-a12084-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-wb-testing-1.jpgAnti-SCML1 Antibody Picoband® Western blot analysis of SCML1 using anti-SCML1 antibody (A12084-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCML1 antigen affinity purified polyclonal antibody (Catalog # A12084-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCML1 at approximately 37 kDa. The expected band size for SCML1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-ihc-testing-2.jpgAnti-SCML1 Antibody Picoband® IHC analysis of SCML1 using anti-SCML1 antibody (A12084-1). SCML1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCML1 Antibody (A12084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-ihc-testing-3.jpgAnti-SCML1 Antibody Picoband® IHC analysis of SCML1 using anti-SCML1 antibody (A12084-1). SCML1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCML1 Antibody (A12084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-ihc-testing-4.jpgAnti-SCML1 Antibody Picoband® IHC analysis of SCML1 using anti-SCML1 antibody (A12084-1). SCML1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCML1 Antibody (A12084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-ihc-testing-5.jpgAnti-SCML1 Antibody Picoband® IHC analysis of SCML1 using anti-SCML1 antibody (A12084-1). SCML1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCML1 Antibody (A12084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-ihc-testing-6.jpgAnti-SCML1 Antibody Picoband® IHC analysis of SCML1 using anti-SCML1 antibody (A12084-1). SCML1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCML1 Antibody (A12084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-ihc-testing-7.jpgAnti-SCML1 Antibody Picoband® IHC analysis of SCML1 using anti-SCML1 antibody (A12084-1). SCML1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCML1 Antibody (A12084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-if-testing-8.jpgAnti-SCML1 Antibody Picoband® IF analysis of SCML1 using anti-SCML1 antibody (A12084-1) and anti-Tubulin Alpha antibody (M03989-3). SCML1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCML1 Antibody (A12084-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-if-testing-9.jpgAnti-SCML1 Antibody Picoband® IF analysis of SCML1 using anti-SCML1 antibody (A12084-1). SCML1 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SCML1 Antibody (A12084-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12084-1-scml1-primary-antibodies-fcm-testing-10.pngAnti-SCML1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SCML1 antibody (A12084-1). Overlay histogram showing U20S cells stained with A12084-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCML1 Antibody (A12084-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-epithelial-sodium-channel-alpha-scnn1a-picoband-trade-antibody-a01413-2-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01413-2-scnn1a-primary-antibodies-wb-testing-1.jpgAnti-epithelial Sodium Channel alpha/SCNN1A Antibody Picoband® Western blot analysis of Epithelial Sodium Channel Alpha/SCNN1A using anti-Epithelial Sodium Channel Alpha/SCNN1A antibody (A01413-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epithelial Sodium Channel Alpha/SCNN1A antigen affinity purified polyclonal antibody (Catalog # A01413-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Epithelial Sodium Channel Alpha/SCNN1A at approximately 70 kDa. The expected band size for Epithelial Sodium Channel Alpha/SCNN1A is at 76 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sco1-picoband-trade-antibody-a02934-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-wb-testing-1.jpgAnti-SCO1 Antibody Picoband® Western blot analysis of SCO1 using anti-SCO1 antibody (A02934-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCO1 antigen affinity purified polyclonal antibody (Catalog # A02934-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCO1 at approximately 30 kDa. The expected band size for SCO1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-ihc-testing-2.jpgAnti-SCO1 Antibody Picoband® IHC analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-ihc-testing-3.jpgAnti-SCO1 Antibody Picoband® IHC analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-ihc-testing-4.jpgAnti-SCO1 Antibody Picoband® IHC analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-ihc-testing-5.jpgAnti-SCO1 Antibody Picoband® IHC analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-ihc-testing-6.jpgAnti-SCO1 Antibody Picoband® IHC analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-ihc-testing-7.jpgAnti-SCO1 Antibody Picoband® IHC analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-ihc-testing-8.jpgAnti-SCO1 Antibody Picoband® IHC analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-fcm-testing-9.pngAnti-SCO1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SCO1 antibody (A02934-1). Overlay histogram showing U87 cells stained with A02934-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCO1 Antibody (A02934-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-if-testing-10.jpgAnti-SCO1 Antibody Picoband® IF analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02934-1-sco1-primary-antibodies-if-testing-11.jpgAnti-SCO1 Antibody Picoband® IF analysis of SCO1 using anti-SCO1 antibody (A02934-1). SCO1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SCO1 Antibody (A02934-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-scyl1-picoband-trade-antibody-a04750-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04750-1-scyl1-primary-antibodies-wb-testing-1.jpgAnti-SCYL1 Antibody Picoband® Western blot analysis of SCYL1 using anti-SCYL1 antibody (A04750-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCYL1 antigen affinity purified polyclonal antibody (Catalog # A04750-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCYL1 at approximately 115 kDa. The expected band size for SCYL1 is at 90 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04750-1-scyl1-primary-antibodies-fcm-testing-2.pngAnti-SCYL1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SCYL1 antibody (A04750-1). Overlay histogram showing HL-60 cells stained with A04750-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCYL1 Antibody (A04750-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-scyl2-picoband-trade-antibody-a08578-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08578-1-scyl2-primary-antibodies-wb-testing-1.jpgAnti-SCYL2 Antibody Picoband® Western blot analysis of SCYL2 using anti-SCYL2 antibody (A08578-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCYL2 antigen affinity purified polyclonal antibody (Catalog # A08578-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCYL2 at approximately 104 kDa. The expected band size for SCYL2 is at 104 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08578-1-scyl2-primary-antibodies-if-testing-2.jpgAnti-SCYL2 Antibody Picoband® IF analysis of SCYL2 using anti-SCYL2 antibody (A08425-1). SCYL2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCYL2 Antibody (A08425-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08578-1-scyl2-primary-antibodies-fcm-testing-3.pngAnti-SCYL2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SCYL2 antibody (A08578-1). Overlay histogram showing U87 cells stained with A08578-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCYL2 Antibody (A08578-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-entr1-picoband-trade-antibody-a31716-1-boster.html2026-03-17T05:13:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31716-1-entr1-primary-antibodies-wb-testing-1.jpgAnti-ENTR1 Antibody Picoband® Western blot analysis of ENTR1 using anti-ENTR1 antibody (A31716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENTR1 antigen affinity purified polyclonal antibody (Catalog # A31716-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENTR1 at approximately 55 kDa. The expected band size for ENTR1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31716-1-entr1-primary-antibodies-fcm-testing-2.pngAnti-ENTR1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-ENTR1 antibody (A31716-1). Overlay histogram showing HL-60 cells stained with A31716-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENTR1 Antibody (A31716-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sde2-picoband-trade-antibody-a15473-1-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-wb-testing-1_1.jpgAnti-SDE2 Antibody Picoband® Western blot analysis of SDE2 using anti-SDE2 antibody (A15473-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDE2 antigen affinity purified polyclonal antibody (Catalog # A15473-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDE2 at approximately 54 kDa. The expected band size for SDE2 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-ihc-testing-2.jpgAnti-SDE2 Antibody Picoband® IHC analysis of SDE2 using anti-SDE2 antibody (A15473-1). SDE2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDE2 Antibody (A15473-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-ihc-testing-3.jpgAnti-SDE2 Antibody Picoband® IHC analysis of SDE2 using anti-SDE2 antibody (A15473-1). SDE2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDE2 Antibody (A15473-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-ihc-testing-4.jpgAnti-SDE2 Antibody Picoband® IHC analysis of SDE2 using anti-SDE2 antibody (A15473-1). SDE2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDE2 Antibody (A15473-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-ihc-testing-5.jpgAnti-SDE2 Antibody Picoband® IHC analysis of SDE2 using anti-SDE2 antibody (A15473-1). SDE2 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDE2 Antibody (A15473-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-ihc-testing-6.jpgAnti-SDE2 Antibody Picoband® IHC analysis of SDE2 using anti-SDE2 antibody (A15473-1). SDE2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDE2 Antibody (A15473-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-if-testing-7.jpgAnti-SDE2 Antibody Picoband® IF analysis of SDE2 using anti-SDE2 antibody (A15473-1). SDE2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SDE2 Antibody (A15473-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15473-1-sde2-primary-antibodies-fcm-testing-8.jpgAnti-SDE2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SDE2 antibody (A15473-1). Overlay histogram showing Jurkat cells stained with A15473-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDE2 Antibody (A15473-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sdf2l1-picoband-trade-antibody-a13464-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-wb-testing-1.jpgAnti-SDF2L1 Antibody Picoband® Western blot analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDF2L1 antigen affinity purified polyclonal antibody (Catalog # A13464) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDF2L1 at approximately 24 kDa. The expected band size for SDF2L1 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-ihc-testing-2.jpgAnti-SDF2L1 Antibody Picoband® IHC analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-ihc-testing-3.jpgAnti-SDF2L1 Antibody Picoband® IHC analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-ihc-testing-4.jpgAnti-SDF2L1 Antibody Picoband® IHC analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-ihc-testing-5.jpgAnti-SDF2L1 Antibody Picoband® IHC analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-ihc-testing-6.jpgAnti-SDF2L1 Antibody Picoband® IHC analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-ihc-testing-7.jpgAnti-SDF2L1 Antibody Picoband® IHC analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-ihc-testing-8.jpgAnti-SDF2L1 Antibody Picoband® IHC analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13464-sdf2l1-primary-antibodies-if-testing-9.jpgAnti-SDF2L1 Antibody Picoband® IF analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). SDF2L1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SDF2L1 Antibody (A13464) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-sec13l1-sec13-picoband-trade-antibody-a04346-1-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04346-1-sec13-primary-antibodies-wb-testing-1_1.jpgAnti-SEC13L1/SEC13 Antibody Picoband® Western blot analysis of SEC13L1/SEC13 using anti-SEC13L1/SEC13 antibody (A04346-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat small intestine tissue lysates, Lane 7: mosue brain tissue lysates, Lane 8: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC13L1/SEC13 antigen affinity purified polyclonal antibody (Catalog # A04346-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC13L1/SEC13 at approximately 36 kDa. The expected band size for SEC13L1/SEC13 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04346-1-sec13-primary-antibodies-ihc-testing-1.jpgAnti-SEC13L1/SEC13 Antibody Picoband®IHC analysis of SEC13 using anti-SEC13 antibody (A04346-1). SEC13 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC13 Antibody (A04346-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04346-1-sec13-primary-antibodies-if-testing-2.jpgAnti-SEC13L1/SEC13 Antibody Picoband® IF analysis of SEC13L1/SEC13 using anti-SEC13L1/SEC13 antibody (A04346-1). SEC13L1/SEC13 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEC13L1/SEC13 Antibody (A04346-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04346-1-sec13-primary-antibodies-fcm-testing-3.pngAnti-SEC13L1/SEC13 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SEC13L1/SEC13 antibody (A04346-1). Overlay histogram showing HL-60 cells stained with A04346-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC13L1/SEC13 Antibody (A04346-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04346-1-sec13-primary-antibodies-fcm-testing-4.pngAnti-SEC13L1/SEC13 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-SEC13L1/SEC13 antibody (A04346-1). Overlay histogram showing JK cells stained with A04346-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC13L1/SEC13 Antibody (A04346-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sec22b-picoband-trade-antibody-a06284-2-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-wb-testing-1_1.jpgAnti-SEC22B Antibody Picoband®Western blot analysis of SEC22B using anti-SEC22B antibody (A06284-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC22B antigen affinity purified polyclonal antibody (A06284-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEC22B at approximately 23 kDa. The expected band size for SEC22B is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-ihc-testing-1.jpgAnti-SEC22B Antibody Picoband®IHC analysis of SEC22B using anti-SEC22B antibody (A06284-2). SEC22B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC22B Antibody (A06284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-ihc-testing-2.jpgAnti-SEC22B Antibody Picoband®IHC analysis of SEC22B using anti-SEC22B antibody (A06284-2). SEC22B was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC22B Antibody (A06284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-ihc-testing-3.jpgAnti-SEC22B Antibody Picoband®IHC analysis of SEC22B using anti-SEC22B antibody (A06284-2). SEC22B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC22B Antibody (A06284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-ihc-testing-4.jpgAnti-SEC22B Antibody Picoband®IHC analysis of SEC22B using anti-SEC22B antibody (A06284-2). SEC22B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC22B Antibody (A06284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-ihc-testing-5.jpgAnti-SEC22B Antibody Picoband®IHC analysis of SEC22B using anti-SEC22B antibody (A06284-2). SEC22B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC22B Antibody (A06284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-if-testing-1.jpgAnti-SEC22B Antibody Picoband®IF analysis of SEC22B using anti-SEC22B antibody (A06284-2). SEC22B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEC22B Antibody (A06284-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-if-testing-2.jpgAnti-SEC22B Antibody Picoband®IF analysis of SEC22B using anti-SEC22B antibody (A06284-2). SEC22B was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEC22B Antibody (A06284-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06284-2-sec22b-primary-antibodies-fcm-testing-1.jpgAnti-SEC22B Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SEC22B antibody (A06284-2). Overlay histogram showing 293T cells stained with A06284-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC22B Antibody (A06284-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sec24c-picoband-trade-antibody-a07405-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07405-sec24c-primary-antibodies-wb-testing-1.jpgAnti-SEC24C Antibody Picoband® Western blot analysis of SEC24C using anti-SEC24C antibody (A07405). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC24C antigen affinity purified polyclonal antibody (Catalog # A07405) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC24C at approximately 118 kDa. The expected band size for SEC24C is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07405-sec24c-primary-antibodies-if-testing-2.jpgAnti-SEC24C Antibody Picoband® IF analysis of SEC24C using anti-SEC24C antibody (A07405). SEC24C was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEC24C Antibody (A07405) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07405-sec24c-primary-antibodies-fcm-testing-3.pngAnti-SEC24C Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SEC24C antibody (A07405). Overlay histogram showing K562 cells stained with A07405 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC24C Antibody (A07405, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sel1l-picoband-trade-antibody-a03343-2-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03343-2-sel1l-primary-antibodies-wb-testing-1.jpgAnti-SEL1L Antibody Picoband® Western blot analysis of SEL1L using anti-SEL1L antibody (A03343-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEL1L antigen affinity purified polyclonal antibody (Catalog # A03343-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEL1L at approximately 100 kDa. The expected band size for SEL1L is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03343-2-sel1l-primary-antibodies-ihc-testing-2.jpgAnti-SEL1L Antibody Picoband® IHC analysis of SEL1L using anti-SEL1L antibody (A03343-2). SEL1L was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEL1L Antibody (A03343-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03343-2-sel1l-primary-antibodies-ihc-testing-3.jpgAnti-SEL1L Antibody Picoband® IHC analysis of SEL1L using anti-SEL1L antibody (A03343-2). SEL1L was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEL1L Antibody (A03343-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03343-2-sel1l-primary-antibodies-if-testing-4.jpgAnti-SEL1L Antibody Picoband® IF analysis of SEL1L using anti-SEL1L antibody (A03343-2). SEL1L was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEL1L Antibody (A03343-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03343-2-sel1l-primary-antibodies-if-testing-5.jpgAnti-SEL1L Antibody Picoband® IF analysis of SEL1L using anti-SEL1L antibody (A03343-2). SEL1L was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEL1L Antibody (A03343-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03343-2-sel1l-primary-antibodies-if-testing-6.jpgAnti-SEL1L Antibody Picoband® IF analysis of SEL1L using anti-SEL1L antibody (A03343-2). SEL1L was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEL1L Antibody (A03343-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03343-2-sel1l-primary-antibodies-fcm-testing-7.pngAnti-SEL1L Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SEL1L antibody (A03343-2). Overlay histogram showing HEL cells stained with A03343-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEL1L Antibody (A03343-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-selenophosphate-synthetase-1-sephs1-picoband-trade-antibody-a10102-1-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10102-1-sephs1-primary-antibodies-wb-testing-1.jpgAnti-Selenophosphate synthetase 1/SEPHS1 Antibody Picoband® Western blot analysis of Selenophosphate Synthetase 1/SEPHS1 using anti-Selenophosphate Synthetase 1/SEPHS1 antibody (A10102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Selenophosphate Synthetase 1/SEPHS1 antigen affinity purified polyclonal antibody (Catalog # A10102-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Selenophosphate Synthetase 1/SEPHS1 at approximately 50 kDa. The expected band size for Selenophosphate Synthetase 1/SEPHS1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10102-1-sephs1-primary-antibodies-ihc-testing-3.jpgAnti-Selenophosphate synthetase 1/SEPHS1 Antibody Picoband®IHC analysis of SEPHS1 using anti-SEPHS1 antibody (A10102-1). SEPHS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEPHS1 Antibody (A10102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10102-1-sephs1-primary-antibodies-ihc-testing-4.jpgAnti-Selenophosphate synthetase 1/SEPHS1 Antibody Picoband®IHC analysis of SEPHS1 using anti-SEPHS1 antibody (A10102-1). SEPHS1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEPHS1 Antibody (A10102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10102-1-sephs1-primary-antibodies-ihc-testing-2.jpgAnti-Selenophosphate synthetase 1/SEPHS1 Antibody Picoband® IHC analysis of Selenophosphate Synthetase 1/SEPHS1 using anti-Selenophosphate Synthetase 1/SEPHS1 antibody (A10102-1). Selenophosphate Synthetase 1/SEPHS1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Selenophosphate Synthetase 1/SEPHS1 Antibody (A10102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-sept6-septin6-picoband-trade-antibody-a10957-4-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10957-4-septin6-primary-antibodies-wb-testing-1.jpgAnti-SEPT6/SEPTIN6 Antibody Picoband® Western blot analysis of SEPT6/SEPTIN6 using anti-SEPT6/SEPTIN6 antibody (A10957-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPT6/SEPTIN6 antigen affinity purified polyclonal antibody (Catalog # A10957-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPT6/SEPTIN6 at approximately 55 kDa. The expected band size for SEPT6/SEPTIN6 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10957-4-septin6-primary-antibodies-ihc-testing-2.jpgAnti-SEPT6/SEPTIN6 Antibody Picoband® IHC analysis of SEPT6/SEPTIN6 using anti-SEPT6/SEPTIN6 antibody (A10957-4). SEPT6/SEPTIN6 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEPT6/SEPTIN6 Antibody (A10957-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10957-4-septin6-primary-antibodies-ihc-testing-3.jpgAnti-SEPT6/SEPTIN6 Antibody Picoband® IHC analysis of SEPT6/SEPTIN6 using anti-SEPT6/SEPTIN6 antibody (A10957-4). SEPT6/SEPTIN6 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEPT6/SEPTIN6 Antibody (A10957-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10957-4-septin6-primary-antibodies-fcm-testing-4.pngAnti-SEPT6/SEPTIN6 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-SEPT6/SEPTIN6 antibody (A10957-4). Overlay histogram showing ANA-1 cells stained with A10957-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT6/SEPTIN6 Antibody (A10957-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10957-4-septin6-primary-antibodies-fcm-testing-5.pngAnti-SEPT6/SEPTIN6 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SEPT6/SEPTIN6 antibody (A10957-4). Overlay histogram showing HEL cells stained with A10957-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEPT6/SEPTIN6 Antibody (A10957-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-septin-11-septin11-picoband-trade-antibody-a12762-3-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12762-3-septin11-primary-antibodies-wb-testing-1.jpgAnti-Septin 11/SEPTIN11 Antibody Picoband® Western blot analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (A12762-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse barin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 11/SEPTIN11 antigen affinity purified polyclonal antibody (Catalog # A12762-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 11/SEPTIN11 at approximately 49 kDa. The expected band size for Septin 11/SEPTIN11 is at 49 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12762-3-septin11-primary-antibodies-ihc-testing-2.jpgAnti-Septin 11/SEPTIN11 Antibody Picoband® IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (A12762-3). Septin 11/SEPTIN11 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 11/SEPTIN11 Antibody (A12762-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12762-3-septin11-primary-antibodies-ihc-testing-3.jpgAnti-Septin 11/SEPTIN11 Antibody Picoband® IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (A12762-3). Septin 11/SEPTIN11 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 11/SEPTIN11 Antibody (A12762-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12762-3-septin11-primary-antibodies-ihc-testing-4.jpgAnti-Septin 11/SEPTIN11 Antibody Picoband® IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (A12762-3). Septin 11/SEPTIN11 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 11/SEPTIN11 Antibody (A12762-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12762-3-septin11-primary-antibodies-ihc-testing-5.jpgAnti-Septin 11/SEPTIN11 Antibody Picoband® IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (A12762-3). Septin 11/SEPTIN11 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 11/SEPTIN11 Antibody (A12762-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12762-3-septin11-primary-antibodies-ihc-testing-6.jpgAnti-Septin 11/SEPTIN11 Antibody Picoband® IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (A12762-3). Septin 11/SEPTIN11 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Septin 11/SEPTIN11 Antibody (A12762-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12762-3-septin11-primary-antibodies-fcm-testing-7.pngAnti-Septin 11/SEPTIN11 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-Septin 11/SEPTIN11 antibody (A12762-3). Overlay histogram showing HEL cells stained with A12762-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Septin 11/SEPTIN11 Antibody (A12762-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-serbp1-picoband-trade-antibody-a04755-2-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04755-2-serbp1-primary-antibodies-wb-testing-1.jpgAnti-SERBP1 Antibody Picoband® Western blot analysis of SERBP1 using anti-SERBP1 antibody (A04755-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERBP1 antigen affinity purified polyclonal antibody (Catalog # A04755-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERBP1 at approximately 55 kDa(human) and 50 kDa(mouse, rat). The expected band size for SERBP1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04755-2-serbp1-primary-antibodies-fcm-testing-2.pngAnti-SERBP1 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-SERBP1 antibody (A04755-2). Overlay histogram showing ANA-1 cells stained with A04755-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERBP1 Antibody (A04755-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04755-2-serbp1-primary-antibodies-fcm-testing-3.pngAnti-SERBP1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SERBP1 antibody (A04755-2). Overlay histogram showing HEL cells stained with A04755-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERBP1 Antibody (A04755-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04755-2-serbp1-primary-antibodies-fcm-testing-4.pngAnti-SERBP1 Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-SERBP1 antibody (A04755-2). Overlay histogram showing RH35 cells stained with A04755-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERBP1 Antibody (A04755-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-serpinb13-picoband-trade-antibody-a09602-1-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09602-1-serpinb13-primary-antibodies-wb-testing-1.jpgAnti-SERPINB13 Antibody Picoband® Western blot analysis of SERPINB13 using anti-SERPINB13 antibody (A09602-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB13 antigen affinity purified polyclonal antibody (Catalog # A09602-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB13 at approximately 44 kDa. The expected band size for SERPINB13 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09602-1-serpinb13-primary-antibodies-fcm-testing-2.pngAnti-SERPINB13 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SERPINB13 antibody (A09602-1). Overlay histogram showing U20S cells stained with A09602-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB13 Antibody (A09602-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sfpq-picoband-trade-antibody-a02243-2-boster.html2026-03-17T05:13:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-wb-testing-1_1.jpgAnti-SFPQ Antibody Picoband®Western blot analysis of SFPQ using anti-SFPQ antibody (A02243-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFPQ antigen affinity purified polyclonal antibody (Catalog # A02243-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFPQ at approximately 100 kDa. The expected band size for SFPQ is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-1.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-2_1.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human stomach lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-3_1.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human aryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-4_1.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-5_1.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-6_1.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-7.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-8.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-9.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-ihc-testing-10.jpgAnti-SFPQ Antibody Picoband®IHC analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-if-testing-1.jpgAnti-SFPQ Antibody Picoband®IF analysis of SFPQ using anti-SFPQ antibody (A02243-2) and anti-Tubulin Alpha antibody (M03989-3). SFPQ was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFPQ Antibody (A02243-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1101) and DyLight® 594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-if-testing-2.jpgAnti-SFPQ Antibody Picoband®IF analysis of SFPQ using anti-SFPQ antibody (A02243-2) and anti-Tubulin Alpha antibody (M03989-3). SFPQ was detected in immunocytochemical section of MG63 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFPQ Antibody (A02243-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1101) and DyLight® 594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-if-testing-3.jpgAnti-SFPQ Antibody Picoband®IF analysis of SFPQ using anti-SFPQ antibody (A02243-2) and anti-Tubulin Alpha antibody (M03989-3). SFPQ was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFPQ Antibody (A02243-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-if-testing-4.jpgAnti-SFPQ Antibody Picoband®IF analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-if-testing-5.jpgAnti-SFPQ Antibody Picoband®IF analysis of SFPQ using anti-SFPQ antibody (A02243-2). SFPQ was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SFPQ Antibody (A02243-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02243-2-sfpq-primary-antibodies-fcm-testing-1.jpgAnti-SFPQ Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SFPQ antibody (A02243-2). Overlay histogram showing 293T cells stained with A02243-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFPQ Antibody (A02243-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sfr1-picoband-trade-antibody-a07683-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07683-1-sfr1-primary-antibodies-wb-testing-1.jpgAnti-SFR1 Antibody Picoband® Western blot analysis of SFR1 using anti-SFR1 antibody (A07683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFR1 antigen affinity purified polyclonal antibody (Catalog # A07683-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFR1 at approximately 22 kDa. The expected band size for SFR1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07683-1-sfr1-primary-antibodies-fcm-testing-2.jpgAnti-SFR1 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-SFR1 antibody (A07683-1). Overlay histogram showing U251 cells stained with A07683-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFR1 Antibody (A07683-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-shcd-shc4-picoband-trade-antibody-a10841-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10841-1-shc4-primary-antibodies-wb-testing-1.jpgAnti-SHCD/SHC4 Antibody Picoband® Western blot analysis of SHCD/SHC4 using anti-SHCD/SHC4 antibody (A10841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCD/SHC4 antigen affinity purified polyclonal antibody (Catalog # A10841-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCD/SHC4 at approximately 69 kDa. The expected band size for SHCD/SHC4 is at 69 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc7a3-picoband-trade-antibody-a09720-3-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09720-3-slc7a3-primary-antibodies-wb-testing-1.jpgAnti-SLC7A3 Antibody Picoband® Western blot analysis of SLC7A3 using anti-SLC7A3 antibody (A09720-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A3 antigen affinity purified polyclonal antibody (Catalog # A09720-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC7A3 at approximately 70 kDa. The expected band size for SLC7A3 is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc22a9-picoband-trade-antibody-a08762-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08762-1-slc22a9-primary-antibodies-wb-testing-1.jpgAnti-SLC22A9 Antibody Picoband® Western blot analysis of SLC22A9 using anti-SLC22A9 antibody (A08762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A9 antigen affinity purified polyclonal antibody (Catalog # A08762-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC22A9 at approximately 70 kDa. The expected band size for SLC22A9 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08762-1-slc22a9-primary-antibodies-fcm-testing-2.pngAnti-SLC22A9 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SLC22A9 antibody (A08762-1). Overlay histogram showing HepG2 cells stained with A08762-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A9 Antibody (A08762-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-smarcd1-picoband-trade-antibody-a07318-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07318-1-smarcd1-primary-antibodies-wb-testing-1.jpgAnti-SMARCD1 Antibody Picoband® Western blot analysis of SMARCD1 using anti-SMARCD1 antibody (A07318-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human MOLT-4 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCD1 antigen affinity purified polyclonal antibody (Catalog # A07318-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCD1 at approximately 58 kDa. The expected band size for SMARCD1 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tgf-beta-receptor-iii-tgfbr3-picoband-trade-antibody-a02765-5-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-5-tgfbr3-primary-antibodies-wb-testing-1.jpgAnti-TGF beta Receptor III/TGFBR3 Antibody Picoband® Western blot analysis of TGF Beta Receptor III/TGFBR3 using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 antigen affinity purified polyclonal antibody (Catalog # A02765-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor III/TGFBR3 at approximately 150 kDa. The expected band size for TGF Beta Receptor III/TGFBR3 is at 93 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02765-5-tgfbr3-primary-antibodies-fcm-testing-2.pngAnti-TGF beta Receptor III/TGFBR3 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-5). Overlay histogram showing Hela cells stained with A02765-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 Antibody (A02765-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tm9sf2-picoband-trade-antibody-a13431-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13431-tm9sf2-primary-antibodies-wb-testing-1.jpgAnti-TM9SF2 Antibody Picoband® Western blot analysis of TM9SF2 using anti-TM9SF2 antibody (A13431). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM9SF2 antigen affinity purified polyclonal antibody (Catalog # A13431) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM9SF2 at approximately 76 kDa. The expected band size for TM9SF2 is at 76 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tmem132a-picoband-trade-antibody-a12054-2-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12054-2-tmem132a-primary-antibodies-wb-testing-1.jpgAnti-TMEM132A Antibody Picoband® Western blot analysis of TMEM132A using anti-TMEM132A antibody (A12054-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM132A antigen affinity purified polyclonal antibody (Catalog # A12054-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM132A at approximately 130 kDa. The expected band size for TMEM132A is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12054-2-tmem132a-primary-antibodies-fcm-testing-2.pngAnti-TMEM132A Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-TMEM132A antibody (A12054-2). Overlay histogram showing SiHa cells stained with A12054-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM132A Antibody (A12054-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-april-tnfsf13-picoband-trade-antibody-a02417-2-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02417-2-tnfsf13-primary-antibodies-wb-testing-1.jpgAnti-APRIL/TNFSF13 Antibody Picoband® Western blot analysis of APRIL/TNFSF13 using anti-APRIL/TNFSF13 antibody (A02417-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APRIL/TNFSF13 antigen affinity purified polyclonal antibody (Catalog # A02417-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APRIL/TNFSF13 at approximately 32 kDa. The expected band size for APRIL/TNFSF13 is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-light-tnfsf14-picoband-trade-antibody-a03516-2-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03516-2-tnfsf14-primary-antibodies-wb-testing-1.jpgAnti-LIGHT/Tnfsf14 Antibody Picoband® Western blot analysis of LIGHT/Tnfsf14 using anti-LIGHT/Tnfsf14 antibody (A03516-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIGHT/Tnfsf14 antigen affinity purified polyclonal antibody (Catalog # A03516-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIGHT/Tnfsf14 at approximately 30 kDa. The expected band size for LIGHT/Tnfsf14 is at 26 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03516-2-tnfsf14-primary-antibodies-ihc-testing-4.jpgAnti-LIGHT/Tnfsf14 Antibody Picoband®IHC analysis of LIGHT/TNFSF14 using anti-LIGHT/TNFSF14 antibody (A03516-2). LIGHT/TNFSF14 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIGHT/TNFSF14 Antibody (A03516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03516-2-tnfsf14-primary-antibodies-ihc-testing-5.jpgAnti-LIGHT/Tnfsf14 Antibody Picoband®IHC analysis of LIGHT/TNFSF14 using anti-LIGHT/TNFSF14 antibody (A03516-2). LIGHT/TNFSF14 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIGHT/TNFSF14 Antibody (A03516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03516-2-tnfsf14-primary-antibodies-ihc-testing-2.jpgAnti-LIGHT/Tnfsf14 Antibody Picoband® IHC analysis of LIGHT/Tnfsf14 using anti-LIGHT/Tnfsf14 antibody (A03516-2). LIGHT/Tnfsf14 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIGHT/Tnfsf14 Antibody (A03516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03516-2-tnfsf14-primary-antibodies-ihc-testing-3.jpgAnti-LIGHT/Tnfsf14 Antibody Picoband® IHC analysis of LIGHT/Tnfsf14 using anti-LIGHT/Tnfsf14 antibody (A03516-2). LIGHT/Tnfsf14 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIGHT/Tnfsf14 Antibody (A03516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03516-2-tnfsf14-primary-antibodies-fcm-testing-4.pngAnti-LIGHT/Tnfsf14 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-LIGHT/Tnfsf14 antibody (A03516-2). Overlay histogram showing ANA-1 cells stained with A03516-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LIGHT/Tnfsf14 Antibody (A03516-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ack1-tnk2-picoband-trade-antibody-a02334-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02334-1-tnk2-primary-antibodies-wb-testing-1.jpgAnti-ACK1/TNK2 Antibody Picoband® Western blot analysis of ACK1/TNK2 using anti-ACK1/TNK2 antibody (A02334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACK1/TNK2 antigen affinity purified polyclonal antibody (Catalog # A02334-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACK1/TNK2 at approximately 115 kDa. The expected band size for ACK1/TNK2 is at 115 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02334-1-tnk2-primary-antibodies-fcm-testing-2.pngAnti-ACK1/TNK2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-ACK1/TNK2 antibody (A02334-1). Overlay histogram showing U20S cells stained with A02334-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACK1/TNK2 Antibody (A02334-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tnni3k-picoband-trade-antibody-a06499-2-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06499-2-tnni3k-primary-antibodies-wb-testing-1.jpgAnti-TNNI3K Antibody Picoband® Western blot analysis of TNNI3K using anti-TNNI3K antibody (A06499-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI3K antigen affinity purified polyclonal antibody (Catalog # A06499-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI3K at approximately 110 kDa. The expected band size for TNNI3K is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06499-2-tnni3k-primary-antibodies-fcm-testing-2.pngAnti-TNNI3K Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TNNI3K antibody (A06499-2). Overlay histogram showing HEL cells stained with A06499-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNNI3K Antibody (A06499-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-toe1-picoband-trade-antibody-a09852-2-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09852-2-toe1-primary-antibodies-ihc-testing-1.jpgAnti-TOE1 Antibody Picoband®IHC analysis of TOE1 using anti-TOE1 antibody (A09852-2). TOE1 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOE1 Antibody (A09852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09852-2-toe1-primary-antibodies-wb-testing-1_1.jpgAnti-TOE1 Antibody Picoband® Western blot analysis of TOE1 using anti-TOE1 antibody (A09852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOE1 antigen affinity purified polyclonal antibody (Catalog # A09852-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOE1 at approximately 57 kDa. The expected band size for TOE1 is at 57 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09852-2-toe1-primary-antibodies-if-testing-2_1.jpgAnti-TOE1 Antibody Picoband® IF analysis of TOE1 using anti-TOE1 antibody (A09852-2) and anti-Tubulin Alpha antibody (M03989-3). TOE1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOE1 Antibody (A09852-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09852-2-toe1-primary-antibodies-fcm-testing-3.jpgAnti-TOE1 Antibody Picoband® Flow Cytometry analysis of Ramos cells using anti-TOE1 antibody (A09852-2). Overlay histogram showing Ramos cells stained with A09852-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOE1 Antibody (A09852-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-tollip-picoband-trade-antibody-a02039-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02039-1-tollip-primary-antibodies-wb-testing-1.jpgAnti-TOLLIP Antibody Picoband® Western blot analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOLLIP antigen affinity purified polyclonal antibody (Catalog # A02039-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOLLIP at approximately 30 kDa. The expected band size for TOLLIP is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02039-1-tollip-primary-antibodies-ihc-testing-2.jpgAnti-TOLLIP Antibody Picoband® IHC analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). TOLLIP was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOLLIP Antibody (A02039-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02039-1-tollip-primary-antibodies-ihc-testing-3.jpgAnti-TOLLIP Antibody Picoband® IHC analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). TOLLIP was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOLLIP Antibody (A02039-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02039-1-tollip-primary-antibodies-ihc-testing-4.jpgAnti-TOLLIP Antibody Picoband® IHC analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). TOLLIP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOLLIP Antibody (A02039-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02039-1-tollip-primary-antibodies-if-testing-5.jpgAnti-TOLLIP Antibody Picoband® IF analysis of TOLLIP using anti-TOLLIP antibody (A02039-1). TOLLIP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOLLIP Antibody (A02039-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02039-1-tollip-primary-antibodies-fcm-testing-6.pngAnti-TOLLIP Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-TOLLIP antibody (A02039-1). Overlay histogram showing Hela cells stained with A02039-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOLLIP Antibody (A02039-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tom1l1-picoband-trade-antibody-a07598-2-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-wb-testing-1.jpgAnti-TOM1L1 Antibody Picoband® Western blot analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOM1L1 antigen affinity purified polyclonal antibody (Catalog # A07598-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOM1L1 at approximately 55 kDa. The expected band size for TOM1L1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-ihc-testing-2.jpgAnti-TOM1L1 Antibody Picoband® IHC analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-ihc-testing-3.jpgAnti-TOM1L1 Antibody Picoband® IHC analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-ihc-testing-4.jpgAnti-TOM1L1 Antibody Picoband® IHC analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-ihc-testing-5.jpgAnti-TOM1L1 Antibody Picoband® IHC analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-ihc-testing-6.jpgAnti-TOM1L1 Antibody Picoband® IHC analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-ihc-testing-7.jpgAnti-TOM1L1 Antibody Picoband® IHC analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-ihc-testing-8.jpgAnti-TOM1L1 Antibody Picoband® IHC analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-if-testing-9.jpgAnti-TOM1L1 Antibody Picoband® IF analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-if-testing-10.jpgAnti-TOM1L1 Antibody Picoband® IF analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). TOM1L1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TOM1L1 Antibody (A07598-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07598-2-tom1l1-primary-antibodies-fcm-testing-11.pngAnti-TOM1L1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-TOM1L1 antibody (A07598-2). Overlay histogram showing JK cells stained with A07598-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOM1L1 Antibody (A07598-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tomm34-picoband-trade-antibody-a06775-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-wb-testing-1.jpgAnti-TOMM34 Antibody Picoband® Western blot analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tisue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM34 antigen affinity purified polyclonal antibody (Catalog # A06775-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM34 at approximately 35 kDa. The expected band size for TOMM34 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-2.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-3.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-4.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-5.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-6.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-7.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-8.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-ihc-testing-9.jpgAnti-TOMM34 Antibody Picoband® IHC analysis of TOMM34 using anti-TOMM34 antibody (A06775-1). TOMM34 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOMM34 Antibody (A06775-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06775-1-tomm34-primary-antibodies-fcm-testing-10.pngAnti-TOMM34 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TOMM34 antibody (A06775-1). Overlay histogram showing Daudi cells stained with A06775-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM34 Antibody (A06775-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-top3b-picoband-trade-antibody-a10446-2-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10446-2-top3b-primary-antibodies-wb-testing-1.jpgAnti-TOP3B Antibody Picoband® Western blot analysis of TOP3B using anti-TOP3B antibody (A10446-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOP3B antigen affinity purified polyclonal antibody (Catalog # A10446-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOP3B at approximately 90-97 kDa. The expected band size for TOP3B is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10446-2-top3b-primary-antibodies-ihc-testing-2.jpgAnti-TOP3B Antibody Picoband® IHC analysis of TOP3B using anti-TOP3B antibody (A10446-2). TOP3B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOP3B Antibody (A10446-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10446-2-top3b-primary-antibodies-ihc-testing-3.jpgAnti-TOP3B Antibody Picoband® IHC analysis of TOP3B using anti-TOP3B antibody (A10446-2). TOP3B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOP3B Antibody (A10446-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10446-2-top3b-primary-antibodies-ihc-testing-4.jpgAnti-TOP3B Antibody Picoband® IHC analysis of TOP3B using anti-TOP3B antibody (A10446-2). TOP3B was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOP3B Antibody (A10446-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-tp53i13-picoband-trade-antibody-a16645-1-boster.html2026-03-17T05:13:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-wb-testing-1.jpgAnti-TP53I13 Antibody Picoband® Western blot analysis of TP53I13 using anti-TP53I13 antibody (A16645-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53I13 antigen affinity purified polyclonal antibody (Catalog # A16645-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53I13 at approximately 55 kDa. The expected band size for TP53I13 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-ihc-testing-2.jpgAnti-TP53I13 Antibody Picoband® IHC analysis of TP53I13 using anti-TP53I13 antibody (A16645-1). TP53I13 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53I13 Antibody (A16645-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-ihc-testing-3.jpgAnti-TP53I13 Antibody Picoband® IHC analysis of TP53I13 using anti-TP53I13 antibody (A16645-1). TP53I13 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53I13 Antibody (A16645-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-ihc-testing-4.jpgAnti-TP53I13 Antibody Picoband® IHC analysis of TP53I13 using anti-TP53I13 antibody (A16645-1). TP53I13 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53I13 Antibody (A16645-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-ihc-testing-5.jpgAnti-TP53I13 Antibody Picoband® IHC analysis of TP53I13 using anti-TP53I13 antibody (A16645-1). TP53I13 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53I13 Antibody (A16645-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-ihc-testing-6.jpgAnti-TP53I13 Antibody Picoband® IHC analysis of TP53I13 using anti-TP53I13 antibody (A16645-1). TP53I13 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53I13 Antibody (A16645-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-ihc-testing-7.jpgAnti-TP53I13 Antibody Picoband® IHC analysis of TP53I13 using anti-TP53I13 antibody (A16645-1). TP53I13 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53I13 Antibody (A16645-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16645-1-tp53i3-primary-antibodies-if-testing-8.jpgAnti-TP53I13 Antibody Picoband® IF analysis of TP53I3 using anti-TP53I3 antibody (A16645-1). TP53I3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TP53I3 Antibody (A16645-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tril-picoband-trade-antibody-a09398-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09398-1-tril-primary-antibodies-wb-testing-1.jpgAnti-TRIL Antibody Picoband® Western blot analysis of TRIL using anti-TRIL antibody (A09398-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIL antigen affinity purified polyclonal antibody (Catalog # A09398-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIL at approximately 89 kDa. The expected band size for TRIL is at 89 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-trim16-picoband-trade-antibody-a09514-5-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-wb-testing-1.jpgAnti-TRIM16 Antibody Picoband® Western blot analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM16 antigen affinity purified polyclonal antibody (Catalog # A09514-5) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM16 at approximately 64 kDa. The expected band size for TRIM16 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-ihc-testing-2.jpgAnti-TRIM16 Antibody Picoband® IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-ihc-testing-3.jpgAnti-TRIM16 Antibody Picoband® IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-ihc-testing-4.jpgAnti-TRIM16 Antibody Picoband® IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-ihc-testing-5.jpgAnti-TRIM16 Antibody Picoband® IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-ihc-testing-6.jpgAnti-TRIM16 Antibody Picoband® IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-ihc-testing-7.jpgAnti-TRIM16 Antibody Picoband® IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-ihc-testing-8.jpgAnti-TRIM16 Antibody Picoband® IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-if-testing-1.jpgAnti-TRIM16 Antibody Picoband®IF analysis of TRIM16 using anti-TRIM16 antibody (A09514-5). TRIM16 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRIM16 Antibody (A09514-5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09514-5-trim16-primary-antibodies-fcm-testing-9.pngAnti-TRIM16 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TRIM16 antibody (A09514-5). Overlay histogram showing Daudi cells stained with A09514-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM16 Antibody (A09514-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trim36-picoband-trade-antibody-a10270-3-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10270-3-trim36-primary-antibodies-wb-testing-1.jpgAnti-TRIM36 Antibody Picoband® Western blot analysis of TRIM36 using anti-TRIM36 antibody (A10270-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM36 antigen affinity purified polyclonal antibody (Catalog # A10270-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM36 at approximately 83 kDa. The expected band size for TRIM36 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10270-3-trim36-primary-antibodies-if-testing-2.jpgAnti-TRIM36 Antibody Picoband® IF analysis of TRIM36 using anti-TRIM36 antibody (A10270-3) and anti-Tubulin Alpha antibody (M03989-3). TRIM36 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRIM36 Antibody (A10270-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10270-3-trim36-primary-antibodies-fcm-testing-3.pngAnti-TRIM36 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-TRIM36 antibody (A10270-3). Overlay histogram showing Hela cells stained with A10270-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM36 Antibody (A10270-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-lin41-trim71-picoband-trade-antibody-a08005-2-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-wb-testing-1.jpgAnti-LIN41/TRIM71 Antibody Picoband® Western blot analysis of LIN41/TRIM71 using anti-LIN41/TRIM71 antibody (A08005-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN41/TRIM71 antigen affinity purified polyclonal antibody (Catalog # A08005-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN41/TRIM71 at approximately 93 kDa. The expected band size for LIN41/TRIM71 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-ihc-testing-2.jpgAnti-LIN41/TRIM71 Antibody Picoband® IHC analysis of LIN41/TRIM71 using anti-LIN41/TRIM71 antibody (A08005-2). LIN41/TRIM71 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIN41/TRIM71 Antibody (A08005-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-ihc-testing-3.jpgAnti-LIN41/TRIM71 Antibody Picoband® IHC analysis of LIN41/TRIM71 using anti-LIN41/TRIM71 antibody (A08005-2). LIN41/TRIM71 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIN41/TRIM71 Antibody (A08005-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-ihc-testing-4.jpgAnti-LIN41/TRIM71 Antibody Picoband® IHC analysis of LIN41/TRIM71 using anti-LIN41/TRIM71 antibody (A08005-2). LIN41/TRIM71 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIN41/TRIM71 Antibody (A08005-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-ihc-testing-5.jpgAnti-LIN41/TRIM71 Antibody Picoband® IHC analysis of LIN41/TRIM71 using anti-LIN41/TRIM71 antibody (A08005-2). LIN41/TRIM71 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIN41/TRIM71 Antibody (A08005-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-ihc-testing-6.jpgAnti-LIN41/TRIM71 Antibody Picoband® IHC analysis of LIN41/TRIM71 using anti-LIN41/TRIM71 antibody (A08005-2). LIN41/TRIM71 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIN41/TRIM71 Antibody (A08005-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-ip-testing-1.jpgAnti-LIN41/TRIM71 Antibody Picoband®Immunoprecipitating (IP) LIN41/TRIM71 in HepG2 whole cell lysate. Western blot analysis of LIN41/TRIM71 using anti-LIN41/TRIM71 antibody (A08005-2); Lane 1: HepG2 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-LIN41/TRIM71 antibody in HepG2 whole cell lysate; Lane 3: anti-LIN41/TRIM71 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LIN41/TRIM71 antigen affinity purified polyclonal antibody (A08005-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for LIN41/TRIM71 at approximately 93 kDa. The expected band size for LIN41/TRIM71 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08005-2-trim7-primary-antibodies-fcm-testing-7.pngAnti-LIN41/TRIM71 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-LIN41/TRIM71 antibody (A08005-2). Overlay histogram showing HEL cells stained with A08005-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LIN41/TRIM71 Antibody (A08005-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-asc1-trip4-picoband-trade-antibody-a07762-2-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07762-2-trip4-primary-antibodies-wb-testing-1.jpgAnti-ASC1/TRIP4 Antibody Picoband® Western blot analysis of ASC1/TRIP4 using anti-ASC1/TRIP4 antibody (A07762-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC1/TRIP4 antigen affinity purified polyclonal antibody (Catalog # A07762-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC1/TRIP4 at approximately 68 kDa. The expected band size for ASC1/TRIP4 is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-trmt2b-picoband-trade-antibody-a16400-2-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16400-2-trmt2b-primary-antibodies-wb-testing-1.jpgAnti-TRMT2B Antibody Picoband® Western blot analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT2B antigen affinity purified polyclonal antibody (Catalog # A16400-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT2B at approximately 47 kDa. The expected band size for TRMT2B is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16400-2-trmt2b-primary-antibodies-ihc-testing-2.jpgAnti-TRMT2B Antibody Picoband® IHC analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). TRMT2B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT2B Antibody (A16400-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16400-2-trmt2b-primary-antibodies-ihc-testing-3.jpgAnti-TRMT2B Antibody Picoband® IHC analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). TRMT2B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT2B Antibody (A16400-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16400-2-trmt2b-primary-antibodies-ihc-testing-4.jpgAnti-TRMT2B Antibody Picoband® IHC analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). TRMT2B was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT2B Antibody (A16400-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16400-2-trmt2b-primary-antibodies-if-testing-5.jpgAnti-TRMT2B Antibody Picoband® IF analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). TRMT2B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT2B Antibody (A16400-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16400-2-trmt2b-primary-antibodies-if-testing-6.jpgAnti-TRMT2B Antibody Picoband® IF analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). TRMT2B was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT2B Antibody (A16400-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16400-2-trmt2b-primary-antibodies-fcm-testing-7.pngAnti-TRMT2B Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TRMT2B antibody (A16400-2). Overlay histogram showing Daudi cells stained with A16400-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT2B Antibody (A16400-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trmt5-picoband-trade-antibody-a11359-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11359-1-trmt5-primary-antibodies-wb-testing-1.jpgAnti-TRMT5 Antibody Picoband® Western blot analysis of TRMT5 using anti-TRMT5 antibody (A11359-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT5 antigen affinity purified polyclonal antibody (Catalog # A11359-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT5 at approximately 50 kDa. The expected band size for TRMT5 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11359-1-trmt5-primary-antibodies-if-testing-2.jpgAnti-TRMT5 Antibody Picoband® IF analysis of TRMT5 using anti-TRMT5 antibody (A11359-1) and anti-Tubulin Alpha antibody (M03989-3). TRMT5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRMT5 Antibody (A11359-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-trnau1ap-picoband-trade-antibody-a15424-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15424-1-trnau1ap-primary-antibodies-wb-testing-1.jpgAnti-TRNAU1AP Antibody Picoband® Western blot analysis of TRNAU1AP using anti-TRNAU1AP antibody (A15424-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNAU1AP antigen affinity purified polyclonal antibody (Catalog # A15424-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNAU1AP at approximately 40 kDa. The expected band size for TRNAU1AP is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-trove2-ss-a-ro60-picoband-trade-antibody-a03428-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03428-1-trove2-primary-antibodies-wb-testing-1.jpgAnti-TROVE2/SS-A/RO60 Antibody Picoband® Western blot analysis of TROVE2/SS-A/RO60 using anti-TROVE2/SS-A/RO60 antibody (A03428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TROVE2/SS-A/RO60 antigen affinity purified polyclonal antibody (Catalog # A03428-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TROVE2/SS-A/RO60 at approximately 60 kDa. The expected band size for TROVE2/SS-A/RO60 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03428-1-trove2-primary-antibodies-fcm-testing-2.pngAnti-TROVE2/SS-A/RO60 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TROVE2/SS-A/RO60 antibody (A03428-1). Overlay histogram showing Daudi cells stained with A03428-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TROVE2/SS-A/RO60 Antibody (A03428-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trp-7-trpc7-picoband-trade-antibody-a04658-2-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04658-2-trpc7-primary-antibodies-wb-testing-1.jpgAnti-TRP 7/TRPC7 Antibody Picoband® Western blot analysis of TRP 7/TRPC7 using anti-TRP 7/TRPC7 antibody (A04658-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SK-N-SH whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRP 7/TRPC7 antigen affinity purified polyclonal antibody (Catalog # A04658-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRP 7/TRPC7 at approximately 100 kDa. The expected band size for TRP 7/TRPC7 is at 100 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-trps1-picoband-trade-antibody-a02328-2-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02328-2-trps1-primary-antibodies-wb-testing-1.jpgAnti-TRPS1 Antibody Picoband® Western blot analysis of TRPS1 using anti-TRPS1 antibody (A02328-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPS1 antigen affinity purified polyclonal antibody (Catalog # A02328-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPS1 at approximately 150 kDa. The expected band size for TRPS1 is at 142 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02328-2-trps1-primary-antibodies-ihc-testing-2.jpgAnti-TRPS1 Antibody Picoband® IHC analysis of TRPS1 using anti-TRPS1 antibody (A02328-2). TRPS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRPS1 Antibody (A02328-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02328-2-trps1-primary-antibodies-if-testing-3.jpgAnti-TRPS1 Antibody Picoband® IF analysis of TRPS1 using anti-TRPS1 antibody (A02328-2) and anti-Tubulin Alpha antibody (M03989-3). TRPS1 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRPS1 Antibody (A02328-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02328-2-trps1-primary-antibodies-fcm-testing-4.pngAnti-TRPS1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-TRPS1 antibody (A02328-2). Overlay histogram showing U20S cells stained with A02328-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPS1 Antibody (A02328-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ef-ts-tsfm-picoband-trade-antibody-a08656-2-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08656-2-tsfm-primary-antibodies-wb-testing-1.jpgAnti-EF-Ts/TSFM Antibody Picoband® Western blot analysis of EF-Ts/TSFM using anti-EF-Ts/TSFM antibody (A08656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EF-Ts/TSFM antigen affinity purified polyclonal antibody (Catalog # A08656-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EF-Ts/TSFM at approximately 35 kDa. The expected band size for EF-Ts/TSFM is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tshz1-picoband-trade-antibody-a08315-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08315-1-tshz1-primary-antibodies-wb-testing-1.jpgAnti-TSHZ1 Antibody Picoband® Western blot analysis of TSHZ1 using anti-TSHZ1 antibody (A08315-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSHZ1 antigen affinity purified polyclonal antibody (Catalog # A08315-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSHZ1 at approximately 118 kDa. The expected band size for TSHZ1 is at 118 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08315-1-tshz1-primary-antibodies-if-testing-2.jpgAnti-TSHZ1 Antibody Picoband® IF analysis of TSHZ1 using anti-TSHZ1 antibody (A08315-1) and anti-Tubulin Alpha antibody (M03989-3). TSHZ1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSHZ1 Antibody (A08315-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tspy1-2-3-4-picoband-trade-antibody-a04215-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04215-1-tspt1-2-3-4-primary-antibodies-wb-testing-1.jpgAnti-TSPY1/2/3/4 Antibody Picoband® Western blot analysis of TSPY1/2/3/4 using anti-TSPY1/2/3/4 antibody (A04215-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPY1/2/3/4 antigen affinity purified polyclonal antibody (Catalog # A04215-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSPY1/2/3/4 at approximately 39 kDa. The expected band size for TSPY1/2/3/4 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04215-1-tspt1-2-3-4-primary-antibodies-fcm-testing-2.pngAnti-TSPY1/2/3/4 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-TSPY1/2/3/4 antibody (A04215-1). Overlay histogram showing U20S cells stained with A04215-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSPY1/2/3/4 Antibody (A04215-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tsr2-picoband-trade-antibody-a12413-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12413-1-tsr2-primary-antibodies-wb-testing-1.jpgAnti-TSR2 Antibody Picoband® Western blot analysis of TSR2 using anti-TSR2 antibody (A12413-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSR2 antigen affinity purified polyclonal antibody (Catalog # A12413-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSR2 at approximately 24-30 kDa. The expected band size for TSR2 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12413-1-tsr2-primary-antibodies-ihc-testing-4.jpgAnti-TSR2 Antibody Picoband®IHC analysis of TSR2 using anti-TSR2 antibody (A12413-1). TSR2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSR2 Antibody (A12413-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12413-1-tsr2-primary-antibodies-ihc-testing-2.jpgAnti-TSR2 Antibody Picoband® IHC analysis of TSR2 using anti-TSR2 antibody (A12413-1). TSR2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSR2 Antibody (A12413-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12413-1-tsr2-primary-antibodies-ihc-testing-3.jpgAnti-TSR2 Antibody Picoband® IHC analysis of TSR2 using anti-TSR2 antibody (A12413-1). TSR2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSR2 Antibody (A12413-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ttc7b-picoband-trade-antibody-a12170-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-wb-testing-1.jpgAnti-TTC7B Antibody Picoband® Western blot analysis of TTC7B using anti-TTC7B antibody (A12170-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC7B antigen affinity purified polyclonal antibody (Catalog # A12170-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC7B at approximately 94 kDa. The expected band size for TTC7B is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-ihc-testing-2.jpgAnti-TTC7B Antibody Picoband® IHC analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-ihc-testing-3.jpgAnti-TTC7B Antibody Picoband® IHC analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-ihc-testing-4.jpgAnti-TTC7B Antibody Picoband® IHC analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-ihc-testing-5.jpgAnti-TTC7B Antibody Picoband® IHC analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-ihc-testing-6.jpgAnti-TTC7B Antibody Picoband® IHC analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-ihc-testing-7.jpgAnti-TTC7B Antibody Picoband® IHC analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-ihc-testing-8.jpgAnti-TTC7B Antibody Picoband® IHC analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-if-testing-9.jpgAnti-TTC7B Antibody Picoband® IF analysis of TTC7B using anti-TTC7B antibody (A12170-1). TTC7B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TTC7B Antibody (A12170-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12170-1-ttc7b-primary-antibodies-fcm-testing-10.pngAnti-TTC7B Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-TTC7B antibody (A12170-1). Overlay histogram showing HepG2 cells stained with A12170-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC7B Antibody (A12170-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tprbk-ttc28-picoband-trade-antibody-a11024-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11024-ttc28-primary-antibodies-wb-testing-1.jpgAnti-TPRBK/TTC28 Antibody Picoband® Western blot analysis of TPRBK/TTC28 using anti-TPRBK/TTC28 antibody (A11024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPRBK/TTC28 antigen affinity purified polyclonal antibody (Catalog # A11024) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPRBK/TTC28 at approximately 271 kDa. The expected band size for TPRBK/TTC28 is at 271 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11024-ttc28-primary-antibodies-fcm-testing-2.pngAnti-TPRBK/TTC28 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TPRBK/TTC28 antibody (A11024). Overlay histogram showing U87 cells stained with A11024 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPRBK/TTC28 Antibody (A11024, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tti2-picoband-trade-antibody-a12943-1-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12943-1-tti2-primary-antibodies-wb-testing-1.jpgAnti-TTI2 Antibody Picoband® Western blot analysis of TTI2 using anti-TTI2 antibody (A12943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTI2 antigen affinity purified polyclonal antibody (Catalog # A12943-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTI2 at approximately 60 kDa. The expected band size for TTI2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12943-1-tti2-primary-antibodies-fcm-testing-2.pngAnti-TTI2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-TTI2 antibody (A12943-1). Overlay histogram showing U251 cells stained with A12943-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTI2 Antibody (A12943-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ttll2-picoband-trade-antibody-a15891-boster.html2026-03-17T05:13:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-wb-testing-1.jpgAnti-TTLL2 Antibody Picoband® Western blot analysis of TTLL2 using anti-TTLL2 antibody (A15891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL2 antigen affinity purified polyclonal antibody (Catalog # A15891) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL2 at approximately 70 kDa. The expected band size for TTLL2 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-2.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-3.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-4.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-5.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-6.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-7.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-8.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-9.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-10.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-ihc-testing-11.jpgAnti-TTLL2 Antibody Picoband® IHC analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-if-testing-12.jpgAnti-TTLL2 Antibody Picoband® IF analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-if-testing-13.jpgAnti-TTLL2 Antibody Picoband® IF analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15891-ttll2-primary-antibodies-if-testing-14.jpgAnti-TTLL2 Antibody Picoband® IF analysis of TTLL2 using anti-TTLL2 antibody (A15891). TTLL2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TTLL2 Antibody (A15891) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ttll3-picoband-trade-antibody-a11845-1-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11845-1-ttll3-primary-antibodies-wb-testing-1.jpgAnti-TTLL3 Antibody Picoband® Western blot analysis of TTLL3 using anti-TTLL3 antibody (A11845-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL3 antigen affinity purified polyclonal antibody (Catalog # A11845-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL3 at approximately 87 kDa. The expected band size for TTLL3 is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ttll12-picoband-trade-antibody-a14589-1-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14589-1-ttll12-primary-antibodies-wb-testing-1.jpgAnti-TTLL12 Antibody Picoband® Western blot analysis of TTLL12 using anti-TTLL12 antibody (A14589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL12 antigen affinity purified polyclonal antibody (Catalog # A14589-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL12 at approximately 75 kDa. The expected band size for TTLL12 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14589-1-ttll12-primary-antibodies-ihc-testing-2.jpgAnti-TTLL12 Antibody Picoband® IHC analysis of TTLL12 using anti-TTLL12 antibody (A14589-1). TTLL12 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL12 Antibody (A14589-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14589-1-ttll12-primary-antibodies-fcm-testing-4.pngAnti-TTLL12 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-TTLL12 antibody (A14589-1). Overlay histogram showing Caco-2 cells stained with A14589-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTLL12 Antibody (A14589-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14589-1-ttll12-primary-antibodies-ihc-testing-3.jpgAnti-TTLL12 Antibody Picoband® IHC analysis of TTLL12 using anti-TTLL12 antibody (A14589-1). TTLL12 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL12 Antibody (A14589-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14589-1-ttll12-primary-antibodies-fcm-testing-5.pngAnti-TTLL12 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-TTLL12 antibody (A14589-1). Overlay histogram showing HEL cells stained with A14589-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTLL12 Antibody (A14589-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tufm-picoband-trade-antibody-a05606-2-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05606-2-tufm-primary-antibodies-wb-testing-1.jpgAnti-TUFM Antibody Picoband® Western blot analysis of TUFM using anti-TUFM antibody (A05606-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUFM antigen affinity purified polyclonal antibody (Catalog # A05606-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUFM at approximately 50 kDa. The expected band size for TUFM is at 46 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05606-2-tufm-primary-antibodies-if-testing-2.jpgAnti-TUFM Antibody Picoband® IF analysis of TUFM using anti-TUFM antibody (A05606-2). TUFM was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TUFM Antibody (A05606-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-alpha-taxilin-txlna-picoband-trade-antibody-a05668-1-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05668-1-txlna-primary-antibodies-wb-testing-1_1.jpgAnti-Alpha Taxilin/TXLNA Antibody Picoband®Western blot analysis of Alpha Taxilin/TXLNA using anti-Alpha Taxilin/TXLNA antibody (A05668-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Taxilin/TXLNA antigen affinity purified polyclonal antibody (A05668-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Alpha Taxilin/TXLNA at approximately 75 kDa. The expected band size for Alpha Taxilin/TXLNA is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05668-1-txlna-primary-antibodies-if-testing-1.jpgAnti-Alpha Taxilin/TXLNA Antibody Picoband®IF analysis of Alpha Taxilin/TXLNA using anti-Alpha Taxilin/TXLNA antibody (A05668-1). Alpha Taxilin/TXLNA was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Alpha Taxilin/TXLNA Antibody (A05668-1) overnight at 4°C. Fluoro550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05668-1-txlna-primary-antibodies-fcm-testing-1.jpgAnti-Alpha Taxilin/TXLNA Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-Alpha Taxilin/TXLNA antibody (A05668-1). Overlay histogram showing HepG2 cells stained with A05668-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Taxilin/TXLNA Antibody (A05668-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-thymidylate-synthase-tyms-picoband-trade-antibody-a04320-4-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-4-tyms-primary-antibodies-wb-testing-1.jpgAnti-Thymidylate Synthase/TYMS Antibody Picoband® Western blot analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thymidylate Synthase/TYMS antigen affinity purified polyclonal antibody (Catalog # A04320-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thymidylate Synthase/TYMS at approximately 36 kDa. The expected band size for Thymidylate Synthase/TYMS is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-4-tyms-primary-antibodies-ihc-testing-2.jpgAnti-Thymidylate Synthase/TYMS Antibody Picoband® IHC analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thymidylate Synthase/TYMS Antibody (A04320-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-4-tyms-primary-antibodies-ihc-testing-3.jpgAnti-Thymidylate Synthase/TYMS Antibody Picoband® IHC analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thymidylate Synthase/TYMS Antibody (A04320-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-4-tyms-primary-antibodies-ihc-testing-4.jpgAnti-Thymidylate Synthase/TYMS Antibody Picoband® IHC analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thymidylate Synthase/TYMS Antibody (A04320-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-4-tyms-primary-antibodies-ihc-testing-5.jpgAnti-Thymidylate Synthase/TYMS Antibody Picoband® IHC analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thymidylate Synthase/TYMS Antibody (A04320-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-4-tyms-primary-antibodies-ihc-testing-6.jpgAnti-Thymidylate Synthase/TYMS Antibody Picoband® IHC analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thymidylate Synthase/TYMS Antibody (A04320-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-4-tyms-primary-antibodies-if-testing-7.jpgAnti-Thymidylate Synthase/TYMS Antibody Picoband® IF analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (A04320-4). Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Thymidylate Synthase/TYMS Antibody (A04320-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/picokine-elisa-kits/human-fgl1-picokine-elisa-kit-ek2201-boster.html2026-03-10T04:35:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2201.jpgHuman FGL1 ELISA Kit PicoKine®Human FGL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-folr3-picokine-elisa-kit-ek2202-boster.html2026-03-10T04:35:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2202.pngHuman FOLR3 ELISA Kit PicoKine®Human FOLR3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fuca1-picokine-elisa-kit-ek2203-boster.html2026-03-10T04:35:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2203.pngHuman FUCA1 ELISA Kit PicoKine®Human FUCA1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gcnt1-picokine-elisa-kit-ek2204-boster.html2026-03-10T04:35:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2204.jpgHuman GCNT1 ELISA Kit PicoKine®Human GCNT1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-acad9-picoband-trade-antibody-a05223-3-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-wb-testing-1.jpgAnti-ACAD9 Antibody Picoband® Western blot analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAD9 antigen affinity purified polyclonal antibody (Catalog # A05223-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAD9 at approximately 69 kDa. The expected band size for ACAD9 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-ihc-testing-2.jpgAnti-ACAD9 Antibody Picoband® IHC analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). ACAD9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACAD9 Antibody (A05223-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-ihc-testing-3.jpgAnti-ACAD9 Antibody Picoband® IHC analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). ACAD9 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACAD9 Antibody (A05223-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-ihc-testing-4.jpgAnti-ACAD9 Antibody Picoband® IHC analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). ACAD9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACAD9 Antibody (A05223-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-ihc-testing-5.jpgAnti-ACAD9 Antibody Picoband® IHC analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). ACAD9 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACAD9 Antibody (A05223-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-ihc-testing-6.jpgAnti-ACAD9 Antibody Picoband® IHC analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). ACAD9 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACAD9 Antibody (A05223-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-ihc-testing-7.jpgAnti-ACAD9 Antibody Picoband® IHC analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). ACAD9 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACAD9 Antibody (A05223-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-if-testing-8.jpgAnti-ACAD9 Antibody Picoband® IF analysis of ACAD9 using anti-ACAD9 antibody (A05223-3). ACAD9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ACAD9 Antibody (A05223-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05223-3-acad9-primary-antibodies-fcm-testing-9.jpgAnti-ACAD9 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-ACAD9 antibody (A05223-3). Overlay histogram showing A549 cells stained with A05223-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACAD9 Antibody (A05223-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atf6-picoband-trade-antibody-a00655-4-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00655-4-atf6-primary-antibodies-wb-testing-1.jpgAnti-ATF6 Antibody Picoband® Western blot analysis of ATF6 using anti-ATF6 antibody (A00655-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. Lane 3: human K562 whole cell lysates. Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF6 antigen affinity purified polyclonal antibody (Catalog # A00655-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF6 at approximately 90-100 kDa. The expected band size for ATF6 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00655-4-atf6-primary-antibodies-ihc-testing-1.jpgAnti-ATF6 Antibody Picoband®IHC analysis of ATF6 using anti-ATF6 antibody (A00655-4). ATF6 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATF6 Antibody (A00655-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00655-4-atf6-primary-antibodies-if-testing-2.jpgAnti-ATF6 Antibody Picoband® IF analysis of ATF6 using anti-ATF6 antibody (A00655-4) and anti-Beta Tubulin antibody (M01857-3). ATF6 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATF6 Antibody (A00655-4) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00655-4-atf6-primary-antibodies-fcm-testing-3.jpgAnti-ATF6 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ATF6 antibody (A00655-4). Overlay histogram showing SiHa cells stained with A00655-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF6 Antibody (A00655-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-milk-fat-globule-1-mfge8-picoband-trade-antibody-a02518-2-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02518-2-mfge8-primary-antibodies-wb-testing-1.jpgAnti-Milk Fat Globule 1/MFGE8 Antibody Picoband® Western blot analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human milk 5 μl, Lane 2: human milk 5 μl, Lane 3: human milk 2.5 μl, Lane 4: human milk 2.5 μl. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Milk Fat Globule 1/MFGE8 antigen affinity purified polyclonal antibody (Catalog # A02518-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Milk Fat Globule 1/MFGE8 at approximately 43 kDa. The expected band size for Milk Fat Globule 1/MFGE8 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02518-2-mfge8-primary-antibodies-ihc-testing-2.jpgAnti-Milk Fat Globule 1/MFGE8 Antibody Picoband® IHC analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-2). Milk Fat Globule 1/MFGE8 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Milk Fat Globule 1/MFGE8 Antibody (A02518-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-milk-fat-globule-1-mfge8-picoband-trade-antibody-a02518-3-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02518-3-mfge8-primary-antibodies-wb-testing-1.jpgAnti-Milk Fat Globule 1/MFGE8 Antibody Picoband® Western blot analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human milk 5 μl, Lane 2: human milk 5 μl, Lane 3: human milk 2.5 μl, Lane 4: human milk 2.5 μl. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Milk Fat Globule 1/MFGE8 antigen affinity purified polyclonal antibody (Catalog # A02518-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Milk Fat Globule 1/MFGE8 at approximately 43 kDa. The expected band size for Milk Fat Globule 1/MFGE8 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02518-3-mfge8-primary-antibodies-ihc-testing-2.jpgAnti-Milk Fat Globule 1/MFGE8 Antibody Picoband® IHC analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-3). Milk Fat Globule 1/MFGE8 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Milk Fat Globule 1/MFGE8 Antibody (A02518-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02518-3-mfge8-primary-antibodies-ihc-testing-3.jpgAnti-Milk Fat Globule 1/MFGE8 Antibody Picoband® IHC analysis of Milk Fat Globule 1/MFGE8 using anti-Milk Fat Globule 1/MFGE8 antibody (A02518-3). Milk Fat Globule 1/MFGE8 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Milk Fat Globule 1/MFGE8 Antibody (A02518-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-enos-nos3-picoband-trade-antibody-a01604-3-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01604-3-nos3-primary-antibodies-wb-testing-1.jpgAnti-eNOS/NOS3 Antibody Picoband® Western blot analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-eNOS/NOS3 antigen affinity purified polyclonal antibody (Catalog # A01604-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for eNOS/NOS3 at approximately 140 kDa. The expected band size for eNOS/NOS3 is at 133 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01604-3-nos3-primary-antibodies-if-testing-2.jpgAnti-eNOS/NOS3 Antibody Picoband® IF analysis of eNOS/NOS3 using anti-eNOS/NOS3 antibody (A01604-3). eNOS/NOS3 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-eNOS/NOS3 Antibody (A01604-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01604-3-nos3-primary-antibodies-fcm-testing-3.jpgAnti-eNOS/NOS3 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-eNOS/NOS3 antibody (A01604-3). Overlay histogram showing U937 cells stained with A01604-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-eNOS/NOS3 Antibody (A01604-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-syntenin-2-sdcbp2-picoband-trade-antibody-a13491-2-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-wb-testing-1.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® Western blot analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntenin 2/SDCBP2 antigen affinity purified polyclonal antibody (Catalog # A13491-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntenin 2/SDCBP2 at approximately 37 kDa. The expected band size for Syntenin 2/SDCBP2 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-ihc-testing-2.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IHC analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Syntenin 2/SDCBP2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-ihc-testing-3.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IHC analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Syntenin 2/SDCBP2 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-ihc-testing-4.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IHC analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Syntenin 2/SDCBP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-ihc-testing-5.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IHC analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Syntenin 2/SDCBP2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-ihc-testing-6.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IHC analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Syntenin 2/SDCBP2 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-ihc-testing-7.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IHC analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Syntenin 2/SDCBP2 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-ihc-testing-8.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IHC analysis of Syntenin 2/SDCBP2 using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Syntenin 2/SDCBP2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-fcm-testing-9.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Overlay histogram showing MCF-7 cells stained with A13491-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-fcm-testing-10.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-Syntenin 2/SDCBP2 antibody (A13491-2). Overlay histogram showing U20S cells stained with A13491-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13491-2-sdcbp2-primary-antibodies-if-testing-11.jpgAnti-Syntenin 2/SDCBP2 Antibody Picoband® IF analysis of Syntenin 2/SDCBP2 and Tubulin beta using anti-Syntenin 2/SDCBP2 antibody (A13491-2) and anti-Tubulin beta antibody (M05613-4). Syntenin 2/SDCBP2 and Tubulin beta was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Syntenin 2/SDCBP2 Antibody (A13491-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-sec23ip-picoband-trade-antibody-a09955-1-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-wb-testing-1.jpgAnti-SEC23IP Antibody Picoband® Western blot analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 292T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC23IP antigen affinity purified polyclonal antibody (Catalog # A09955-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC23IP at approximately 125 kDa. The expected band size for SEC23IP is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-2.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-3.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-4.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-5.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-6.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-7.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-8.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-9.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-10.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-11.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-12.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-13.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-ihc-testing-14.jpgAnti-SEC23IP Antibody Picoband® IHC analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-if-testing-15.jpgAnti-SEC23IP Antibody Picoband® IF analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-if-testing-16.jpgAnti-SEC23IP Antibody Picoband® IF analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). SEC23IP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEC23IP Antibody (A09955-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09955-1-sec23ip-primary-antibodies-fcm-testing-17.jpgAnti-SEC23IP Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SEC23IP antibody (A09955-1). Overlay histogram showing HL-60 cells stained with A09955-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC23IP Antibody (A09955-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sec24a-picoband-trade-antibody-a09726-1-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09726-1-sec24a-primary-antibodies-wb-testing-1.jpgAnti-SEC24A Antibody Picoband® Western blot analysis of SEC24A using anti-SEC24A antibody (A09726-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 292T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC24A antigen affinity purified polyclonal antibody (Catalog # A09726-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC24A at approximately 120 kDa. The expected band size for SEC24A is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09726-1-sec24a-primary-antibodies-fcm-testing-2.jpgAnti-SEC24A Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SEC24A antibody (A09726-1). Overlay histogram showing HEL cells stained with A09726-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC24A Antibody (A09726-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sec31a-picoband-trade-antibody-a04582-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-wb-testing-1.jpgAnti-SEC31A Antibody Picoband® Western blot analysis of SEC31A using anti-SEC31A antibody (A04582). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC31A antigen affinity purified polyclonal antibody (Catalog # A04582) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC31A at approximately 133 kDa. The expected band size for SEC31A is at 133 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-2.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-3.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-4.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-5.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-6.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-7.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-8.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-9.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-10.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-11.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-12.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-ihc-testing-13.jpgAnti-SEC31A Antibody Picoband® IHC analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-if-testing-14.jpgAnti-SEC31A Antibody Picoband® IF analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-if-testing-15.jpgAnti-SEC31A Antibody Picoband® IF analysis of SEC31A using anti-SEC31A antibody (A04582). SEC31A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEC31A Antibody (A04582) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04582-sec31a-primary-antibodies-fcm-testing-16.jpgAnti-SEC31A Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SEC31A antibody (A04582). Overlay histogram showing HL-60 cells stained with A04582 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC31A Antibody (A04582, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sel1l2-picoband-trade-antibody-a15836-1-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15836-1-sel1l2-primary-antibodies-wb-testing-1.jpgAnti-SEL1L2 Antibody Picoband® Western blot analysis of SEL1L2 using anti-SEL1L2 antibody (A15836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEL1L2 antigen affinity purified polyclonal antibody (Catalog # A15836-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEL1L2 at approximately 70 kDa. The expected band size for SEL1L2 is at 78 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sbp-selenbp1-picoband-trade-antibody-a04444-3-boster.html2026-03-17T05:14:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-wb-testing-1.jpgAnti-SBP/SELENBP1 Antibody Picoband® Western blot analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SBP/SELENBP1 antigen affinity purified polyclonal antibody (Catalog # A04444-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SBP/SELENBP1 at approximately 52-56 kDa. The expected band size for SBP/SELENBP1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-9.jpgAnti-SBP/SELENBP1 Antibody Picoband®IHC analysis of SELENBP1 using anti-SELENBP1 antibody (A04444-3). SELENBP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-10.jpgAnti-SBP/SELENBP1 Antibody Picoband®IHC analysis of SELENBP1 using anti-SELENBP1 antibody (A04444-3). SELENBP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-2.jpgAnti-SBP/SELENBP1 Antibody Picoband® IHC analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-3.jpgAnti-SBP/SELENBP1 Antibody Picoband® IHC analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-4.jpgAnti-SBP/SELENBP1 Antibody Picoband® IHC analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-5.jpgAnti-SBP/SELENBP1 Antibody Picoband® IHC analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-6.jpgAnti-SBP/SELENBP1 Antibody Picoband® IHC analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-7.jpgAnti-SBP/SELENBP1 Antibody Picoband® IHC analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-ihc-testing-8.jpgAnti-SBP/SELENBP1 Antibody Picoband® IHC analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-if-testing-9.jpgAnti-SBP/SELENBP1 Antibody Picoband® IF analysis of SBP/SELENBP1 using anti-SBP/SELENBP1 antibody (A04444-3). SBP/SELENBP1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SBP/SELENBP1 Antibody (A04444-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04444-3-selenbp1-primary-antibodies-fcm-testing-10.jpgAnti-SBP/SELENBP1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SBP/SELENBP1 antibody (A04444-3). Overlay histogram showing 293T cells stained with A04444-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SBP/SELENBP1 Antibody (A04444-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-selenot-picoband-trade-antibody-a31970-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-wb-testing-1.jpgAnti-SELENOT Antibody Picoband® Western blot analysis of SELENOT using anti-SELENOT antibody (A31970). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: rat pancreas tissue lysates, Lane 8: mouse pancreas tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SELENOT antigen affinity purified polyclonal antibody (Catalog # A31970) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SELENOT at approximately 22 kDa. The expected band size for SELENOT is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-2.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-3.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-4.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-5.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-6.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-7.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-8.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-9.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-10.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-ihc-testing-11.jpgAnti-SELENOT Antibody Picoband® IHC analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-if-testing-12.jpgAnti-SELENOT Antibody Picoband® IF analysis of SELENOT using anti-SELENOT antibody (A31970). SELENOT was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SELENOT Antibody (A31970) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31970-selenot-primary-antibodies-fcm-testing-13.jpgAnti-SELENOT Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SELENOT antibody (A31970). Overlay histogram showing HL-60 cells stained with A31970 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SELENOT Antibody (A31970, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sema4f-picoband-trade-antibody-a11873-2-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-wb-testing-1.jpgAnti-SEMA4F Antibody Picoband® Western blot analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA4F antigen affinity purified polyclonal antibody (Catalog # A11873-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA4F at approximately 84 kDa. The expected band size for SEMA4F is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-11.jpgAnti-SEMA4F Antibody Picoband®IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-2.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-3.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-4.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-5.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-6.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-7.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-8.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-9.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-ihc-testing-10.jpgAnti-SEMA4F Antibody Picoband® IHC analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-if-testing-11.jpgAnti-SEMA4F Antibody Picoband® IF analysis of SEMA4F using anti-SEMA4F antibody (A11873-2). SEMA4F was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEMA4F Antibody (A11873-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11873-2-sema4f-primary-antibodies-fcm-testing-12.jpgAnti-SEMA4F Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-SEMA4F antibody (A11873-2). Overlay histogram showing Daudi cells stained with A11873-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SEMA4F Antibody (A11873-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-semaphorin-7a-sema7a-picoband-trade-antibody-a03832-2-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03832-2-sema7a-primary-antibodies-wb-testing-1.jpgAnti-Semaphorin 7a/SEMA7A Antibody Picoband® Western blot analysis of Semaphorin 7a/SEMA7A using anti-Semaphorin 7a/SEMA7A antibody (A03832-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Semaphorin 7a/SEMA7A antigen affinity purified polyclonal antibody (Catalog # A03832-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Semaphorin 7a/SEMA7A at approximately 75 kDa. The expected band size for Semaphorin 7a/SEMA7A is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03832-2-sema7a-primary-antibodies-fcm-testing-2.jpgAnti-Semaphorin 7a/SEMA7A Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-Semaphorin 7a/SEMA7A antibody (A03832-2). Overlay histogram showing HEL cells stained with A03832-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Semaphorin 7a/SEMA7A Antibody (A03832-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-septin12-picoband-trade-antibody-a12342-2-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12342-2-septin12-primary-antibodies-wb-testing-1.jpgAnti-SEPTIN12 Antibody Picoband® Western blot analysis of SEPTIN12 using anti-SEPTIN12 antibody (A12342-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPTIN12 antigen affinity purified polyclonal antibody (Catalog # A12342-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPTIN12 at approximately 45 kDa. The expected band size for SEPTIN12 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-serac1-picoband-trade-antibody-a08852-2-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-wb-testing-1_1_1.jpgAnti-SERAC1 Antibody Picoband® Western blot analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERAC1 antigen affinity purified polyclonal antibody (Catalog # A08852-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERAC1 at approximately 74, 20 kDa. The expected band size for SERAC1 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-2.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-3.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-4.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-5.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-6.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-7.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-8.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of human lung adenocarcinom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-9.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-ihc-testing-10.jpgAnti-SERAC1 Antibody Picoband® IHC analysis of SERAC1 using anti-SERAC1 antibody (A08852-2). SERAC1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERAC1 Antibody (A08852-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-fcm-testing-11_1.jpgAnti-SERAC1 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SERAC1 antibody (A08852-2). Overlay histogram showing Jurkat cells stained with A08852-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERAC1 Antibody (A08852-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08852-2-serac1-primary-antibodies-fcm-testing-12.jpgAnti-SERAC1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SERAC1 antibody (A08852-2). Overlay histogram showing U20S cells stained with A08852-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERAC1 Antibody (A08852-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-serinc2-picoband-trade-antibody-a14175-2-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14175-2-serinc2-primary-antibodies-wb-testing-1.jpgAnti-SERINC2 Antibody Picoband® Western blot analysis of SERINC2 using anti-SERINC2 antibody (A14175-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERINC2 antigen affinity purified polyclonal antibody (Catalog # A14175-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERINC2 at approximately 65 kDa. The expected band size for SERINC2 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14175-2-serinc2-primary-antibodies-if-testing-2.jpgAnti-SERINC2 Antibody Picoband® IF analysis of SERINC2 using anti-SERINC2 antibody (A14175-2). SERINC2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SERINC2 Antibody (A14175-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14175-2-serinc2-primary-antibodies-fcm-testing-3.jpgAnti-SERINC2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-SERINC2 antibody (A14175-2). Overlay histogram showing A431 cells stained with A14175-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SERINC2 Antibody (A14175-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-serinc5-picoband-trade-antibody-a06603-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06603-serinc5-primary-antibodies-wb-testing-1.jpgAnti-SERINC5 Antibody Picoband® Western blot analysis of SERINC5 using anti-SERINC5 antibody (A06603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERINC5 antigen affinity purified polyclonal antibody (Catalog # A06603) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERINC5 at approximately 65 kDa. The expected band size for SERINC5 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06603-serinc5-primary-antibodies-fcm-testing-2.jpgAnti-SERINC5 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SERINC5 antibody (A06603). Overlay histogram showing HepG2 cells stained with A06603 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERINC5 Antibody (A06603, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06603-serinc5-primary-antibodies-fcm-testing-3.jpgAnti-SERINC5 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SERINC5 antibody (A06603). Overlay histogram showing PC-3 cells stained with A06603 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERINC5 Antibody (A06603, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06603-serinc5-primary-antibodies-wb-testing-4.jpgAnti-SERINC5 Antibody Picoband® Western blot analysis of SERINC5 using anti-SERINC5 antibody (A06603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: transfected HEK293 with plvp, Lane 2: transfected HEK293 with CDNA serinc5. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk in 1X TBST for 1hr in RT. The membrane was incubated with rabbit anti-SERINC5 antigen affinity purified polyclonal antibody (A06603) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:3000 for 30mins at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SERINC5 at approximately 65 kDa. The expected band size for SERINC5 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-aact-serpina3-picoband-trade-antibody-a02312-3-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02312-3-serpina3-primary-antibodies-wb-testing-1.jpgAnti-AACT/SERPINA3 Antibody Picoband® Western blot analysis of AACT/SERPINA3 using anti-AACT/SERPINA3 antibody (A02312-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human HUH-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AACT/SERPINA3 antigen affinity purified polyclonal antibody (Catalog # A02312-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AACT/SERPINA3 at approximately 50 kDa. The expected band size for AACT/SERPINA3 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02312-3-serpina3-primary-antibodies-fcm-testing-2.jpgAnti-AACT/SERPINA3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-AACT/SERPINA3 antibody (A02312-3). Overlay histogram showing HepG2 cells stained with A02312-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AACT/SERPINA3 Antibody (A02312-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-thyroxine-binding-globulin-serpina7-picoband-trade-antibody-a02766-1-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02766-1-serpina7-primary-antibodies-wb-testing-1.jpgAnti-Thyroxine Binding Globulin/SERPINA7 Antibody Picoband® Western blot analysis of Thyroxine Binding Globulin/SERPINA7 using anti-Thyroxine Binding Globulin/SERPINA7 antibody (A02766-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thyroxine Binding Globulin/SERPINA7 antigen affinity purified polyclonal antibody (Catalog # A02766-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thyroxine Binding Globulin/SERPINA7 at approximately 50 kDa. The expected band size for Thyroxine Binding Globulin/SERPINA7 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02766-1-serpina7-primary-antibodies-ihc-testing-2.jpgAnti-Thyroxine Binding Globulin/SERPINA7 Antibody Picoband® IHC analysis of Thyroxine Binding Globulin/SERPINA7 using anti-Thyroxine Binding Globulin/SERPINA7 antibody (A02766-1). Thyroxine Binding Globulin/SERPINA7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thyroxine Binding Globulin/SERPINA7 Antibody (A02766-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02766-1-serpina7-primary-antibodies-fcm-testing-3.jpgAnti-Thyroxine Binding Globulin/SERPINA7 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Thyroxine Binding Globulin/SERPINA7 antibody (A02766-1). Overlay histogram showing HepG2 cells stained with A02766-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Thyroxine Binding Globulin/SERPINA7 Antibody (A02766-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-zpi-serpina10-picoband-trade-antibody-a05576-2-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05576-2-serpina10-primary-antibodies-wb-testing-1.jpgAnti-ZPI/SERPINA10 Antibody Picoband® Western blot analysis of SERPINA10 using anti-SERPINA10 antibody (A05576-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA10 antigen affinity purified polyclonal antibody (Catalog # A05576-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA10 at approximately 71 kDa. The expected band size for SERPINA10 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05576-2-serpina10-primary-antibodies-fcm-testing-2.jpgAnti-ZPI/SERPINA10 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-ZPI/SERPINA10 antibody (A05576-2). Overlay histogram showing HEL cells stained with A05576-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZPI/SERPINA10 Antibody (A05576-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05576-2-serpina10-primary-antibodies-fcm-testing-3.jpgAnti-ZPI/SERPINA10 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ZPI/SERPINA10 antibody (A05576-2). Overlay histogram showing RT4 cells stained with A05576-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZPI/SERPINA10 Antibody (A05576-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pi2-serpinb1-picoband-trade-antibody-a04491-2-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04491-2-serpinb1-primary-antibodies-wb-testing-1.jpgAnti-PI2/SERPINB1 Antibody Picoband® Western blot analysis of PI2/SERPINB1 using anti-PI2/SERPINB1 antibody (A04491-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI2/SERPINB1 antigen affinity purified polyclonal antibody (Catalog # A04491-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI2/SERPINB1 at approximately 43 kDa. The expected band size for PI2/SERPINB1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04491-2-serpinb1-primary-antibodies-if-testing-2.jpgAnti-PI2/SERPINB1 Antibody Picoband® IF analysis of PI2/SERPINB1 using anti-PI2/SERPINB1 antibody (A04491-2). PI2/SERPINB1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PI2/SERPINB1 Antibody (A04491-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04491-2-serpinb1-primary-antibodies-fcm-testing-3.jpgAnti-PI2/SERPINB1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PI2/SERPINB1 antibody (A04491-2). Overlay histogram showing SiHa cells stained with A04491-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI2/SERPINB1 Antibody (A04491-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04491-2-serpinb1-primary-antibodies-fcm-testing-4.jpgAnti-PI2/SERPINB1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PI2/SERPINB1 antibody (A04491-2). Overlay histogram showing THP-1 cells stained with A04491-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI2/SERPINB1 Antibody (A04491-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-antithrombin-iii-serpinc1-picoband-trade-antibody-a00469-1-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00469-1-serpinc1-primary-antibodies-wb-testing-1.jpgAnti-Antithrombin III/SERPINC1 Antibody Picoband® Western blot analysis of Antithrombin III/SERPINC1 using anti-Antithrombin III/SERPINC1 antibody (A00469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Antithrombin III/SERPINC1 antigen affinity purified polyclonal antibody (Catalog # A00469-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Antithrombin III/SERPINC1 at approximately 53 kDa. The expected band size for Antithrombin III/SERPINC1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00469-1-serpinc1-primary-antibodies-fcm-testing-2.jpgAnti-Antithrombin III/SERPINC1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Antithrombin III/SERPINC1 antibody (A00469-1). Overlay histogram showing HepG2 cells stained with A00469-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Antithrombin III/SERPINC1 Antibody (A00469-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-serpine3-picoband-trade-antibody-a18879-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18879-serpine3-primary-antibodies-wb-testing-1.jpgAnti-SERPINE3 Antibody Picoband® Western blot analysis of SERPINE3 using anti-SERPINE3 antibody (A18879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINE3 antigen affinity purified polyclonal antibody (Catalog # A18879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINE3 at approximately 52 kDa. The expected band size for SERPINE3 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18879-12974_2024_3062_fig2_html.pngAnti-SERPINE3 Antibody Picoband®Temporal gene and protein expression changes after retinal detachment and sequencing validation. ( A ) Heatmap showing significantly upregulated (FC ≥ 1.5, p adj ≤0.05, red) and downregulated (FC ≤ 0.67, p adj ≤0.05, blue) DEGs 1 and 7 dprd. ( B-F ) Relative expression values of DEG grouped by temporal expression. ( B ) Log2FC of all DEGs significantly upregulated at both 1 and 7 dprd (Up/Up). ( C ) Log2FC of DEGs significantly upregulated only at 1 dprd (Up/NSC). Gray indicates DEG that were not significantly changed (NSC). ( D ) Log2FC of DEGs significantly upregulated only at 7 dprd (NSC/Up). ( E ) Log2FC of DEGs significantly downregulated only at 1 dprd (Down/NSC). ( F ) Log2FC of DEGs significantly downregulated at both 1 and 7 dprd (Down/Down). ( G-K ) QRT-PCR of temporal DEG expression changes after retinal detachment for RNA-Seq validation. Relative mRNA expression of ( G ) Grem1 , ( H ) S100a8 , ( I ) Serpine3 , ( J ) Rpe65 , and ( K ) Tsc22d3 , obtained from isolated naïve RPE ( n = 6), as well RD RPE at 1 dprd ( n = 6) and 7 dprd ( n = 6). Bar graphs represent mean ± SEM. Statistical analysis was performed using one-way ANOVA with repeated measures followed by Tukey’s post hoc test. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001 Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-024-03062-2'>38528525</a> https://www.bosterbio.com/products/primary-antibodies/anti-setd3-picoband-trade-antibody-a10965-1-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-wb-testing-1.jpgAnti-SETD3 Antibody Picoband® Western blot analysis of SETD3 using anti-SETD3 antibody (A10965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETD3 antigen affinity purified polyclonal antibody (Catalog # A10965-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETD3 at approximately 60 kDa. The expected band size for SETD3 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-wb-testing-2.jpgAnti-SETD3 Antibody Picoband® Western blot analysis of SETD3 using anti-SETD3 antibody (A10965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat H9C2(2-1) whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse C2C12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETD3 antigen affinity purified polyclonal antibody (Catalog # A10965-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETD3 at approximately 60 kDa. The expected band size for SETD3 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-ihc-testing-3.jpgAnti-SETD3 Antibody Picoband® IHC analysis of SETD3 using anti-SETD3 antibody (A10965-1). SETD3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SETD3 Antibody (A10965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-ihc-testing-4.jpgAnti-SETD3 Antibody Picoband® IHC analysis of SETD3 using anti-SETD3 antibody (A10965-1). SETD3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SETD3 Antibody (A10965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-ihc-testing-5.jpgAnti-SETD3 Antibody Picoband® IHC analysis of SETD3 using anti-SETD3 antibody (A10965-1). SETD3 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SETD3 Antibody (A10965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-ihc-testing-6.jpgAnti-SETD3 Antibody Picoband® IHC analysis of SETD3 using anti-SETD3 antibody (A10965-1). SETD3 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SETD3 Antibody (A10965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-ihc-testing-7.jpgAnti-SETD3 Antibody Picoband® IHC analysis of SETD3 using anti-SETD3 antibody (A10965-1). SETD3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SETD3 Antibody (A10965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-if-testing-8.jpgAnti-SETD3 Antibody Picoband® IF analysis of SETD3 using anti-SETD3 antibody (A10965-1). SETD3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SETD3 Antibody (A10965-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-if-testing-9.jpgAnti-SETD3 Antibody Picoband® IF analysis of SETD3 using anti-SETD3 antibody (A10965-1). SETD3 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SETD3 Antibody (A10965-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-fcm-testing-10.jpgAnti-SETD3 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SETD3 antibody (A10965-1). Overlay histogram showing 293T cells stained with A10965-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SETD3 Antibody (A10965-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10965-1-setd3-primary-antibodies-fcm-testing-11.jpgAnti-SETD3 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SETD3 antibody (A10965-1). Overlay histogram showing HL-60 cells stained with A10965-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SETD3 Antibody (A10965-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sf3a1-picoband-trade-antibody-a05483-1-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-wb-testing-1.jpgAnti-SF3A1 Antibody Picoband® Western blot analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A1 antigen affinity purified polyclonal antibody (Catalog # A05483-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A1 at approximately 120-130 kDa. The expected band size for SF3A1 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-wb-testing-2.jpgAnti-SF3A1 Antibody Picoband® Western blot analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A1 antigen affinity purified polyclonal antibody (Catalog # A05483-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A1 at approximately 120-130 kDa. The expected band size for SF3A1 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ihc-testing-3.jpgAnti-SF3A1 Antibody Picoband® IHC analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ihc-testing-4.jpgAnti-SF3A1 Antibody Picoband® IHC analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ihc-testing-5.jpgAnti-SF3A1 Antibody Picoband® IHC analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ihc-testing-6.jpgAnti-SF3A1 Antibody Picoband® IHC analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ihc-testing-7.jpgAnti-SF3A1 Antibody Picoband® IHC analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ihc-testing-8.jpgAnti-SF3A1 Antibody Picoband® IHC analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ihc-testing-9.jpgAnti-SF3A1 Antibody Picoband® IHC analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-if-testing-10.jpgAnti-SF3A1 Antibody Picoband® IF analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-if-testing-11.jpgAnti-SF3A1 Antibody Picoband® IF analysis of SF3A1 using anti-SF3A1 antibody (A05483-1). SF3A1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3A1 Antibody (A05483-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-if-testing-12.jpgAnti-SF3A1 Antibody Picoband® IF analysis of SF3A1 and Tubulin alpha using anti-SF3A1 antibody (A05483-1) and anti-Tubulin alpha antibody (M03989-3). SF3A1 and Tubulin alpha were detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SF3A1 antibody (A05483-1) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05483-1-sf3a1-primary-antibodies-ip-testing-1.jpgAnti-SF3A1 Antibody Picoband®Immunoprecipitating (IP) SF3A1 in Hela whole cell lysate. Western blot analysis of SF3A1 using anti-SF3A1 antibody (A05483-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SF3A1 antibody in Hela whole cell lysate; Lane 3: anti-SF3A1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SF3A1 antigen affinity purified polyclonal antibody (A05483-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SF3A1 at approximately 120 kDa. The expected band size for SF3A1 is at 89 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sf3a3-picoband-trade-antibody-a10671-1-boster.html2026-03-17T05:14:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-wb-testing-1.jpgAnti-SF3A3 Antibody Picoband® Western blot analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A3 antigen affinity purified polyclonal antibody (Catalog # A10671-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A3 at approximately 59 kDa. The expected band size for SF3A3 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-2.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-3.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-4.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-5.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-6.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-7.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-8.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-9.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-10.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ihc-testing-11.jpgAnti-SF3A3 Antibody Picoband® IHC analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-if-testing-12.jpgAnti-SF3A3 Antibody Picoband® IF analysis of SF3A3 using anti-SF3A3 antibody (A10671-1). SF3A3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3A3 Antibody (A10671-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-if-testing-13.jpgAnti-SF3A3 Antibody Picoband® IF analysis of SF3A3 and Tubulin alpha using anti-SF3A3 antibody (A10671-1) and anti-Tubulin alpha antibody (M03989-3). SF3A3 and Tubulin alpha were detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SF3A3 antibody (A10671-1) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-ip-testing-1.jpgAnti-SF3A3 Antibody Picoband®Immunoprecipitating (IP) SF3A3 in Hela whole cell lysate. Western blot analysis of SF3A3 using anti-SF3A3 antibody (A10671-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SF3A3 antibody in Hela whole cell lysate; Lane 3: anti-SF3A3 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SF3A3 antigen affinity purified polyclonal antibody (A10671-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SF3A3 at approximately 59 kDa. The expected band size for SF3A3 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10671-1-sf3a3-primary-antibodies-fcm-testing-14.jpgAnti-SF3A3 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SF3A3 antibody (A10671-1). Overlay histogram showing SiHa cells stained with A10671-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SF3A3 Antibody (A10671-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sf3b2-picoband-trade-antibody-a07047-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07047-1-sf3b2-primary-antibodies-wb-testing-1.jpgAnti-SF3B2 Antibody Picoband® Western blot analysis of SF3B2 using anti-SF3B2 antibody (A07047-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH-35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B2 antigen affinity purified polyclonal antibody (Catalog # A07047-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B2 at approximately 120 kDa. The expected band size for SF3B2 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07047-1-sf3b2-primary-antibodies-fcm-testing-2.jpgAnti-SF3B2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SF3B2 antibody (A07047-1). Overlay histogram showing SiHa cells stained with A07047-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SF3B2 Antibody (A07047-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sf3b3-picoband-trade-antibody-a08240-3-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-wb-testing-1.jpgAnti-SF3B3 Antibody Picoband® Western blot analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B3 antigen affinity purified polyclonal antibody (Catalog # A08240-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B3 at approximately 136 kDa. The expected band size for SF3B3 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-2.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-3.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-4.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-5.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-6.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-7.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-8.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-9.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-10.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-11.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-12.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-13.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-14.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-ihc-testing-15.jpgAnti-SF3B3 Antibody Picoband® IHC analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of rat skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-if-testing-16.jpgAnti-SF3B3 Antibody Picoband® IF analysis of SF3B3 and Tubulin alpha using anti-SF3B3 antibody (A08240-3) and anti-Tubulin alpha antibody (M03989-3). SF3B3 and Tubulin alpha were detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SF3B3 antibody (A08240-3) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-if-testing-17.jpgAnti-SF3B3 Antibody Picoband® IF analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-if-testing-18.jpgAnti-SF3B3 Antibody Picoband® IF analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-if-testing-19.jpgAnti-SF3B3 Antibody Picoband® IF analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). SF3B3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3B3 Antibody (A08240-3) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08240-3-sf3b3-primary-antibodies-fcm-testing-20.jpgAnti-SF3B3 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SF3B3 antibody (A08240-3). Overlay histogram showing A549 cells stained with A08240-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SF3B3 Antibody (A08240-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sfxn3-picoband-trade-antibody-a14648-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-wb-testing-1.jpgAnti-SFXN3 Antibody Picoband® Western blot analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFXN3 antigen affinity purified polyclonal antibody (Catalog # A14648-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFXN3 at approximately 36 kDa. The expected band size for SFXN3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-ihc-testing-2.jpgAnti-SFXN3 Antibody Picoband® IHC analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). SFXN3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN3 Antibody (A14648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-ihc-testing-3.jpgAnti-SFXN3 Antibody Picoband® IHC analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). SFXN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN3 Antibody (A14648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-ihc-testing-4.jpgAnti-SFXN3 Antibody Picoband® IHC analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). SFXN3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN3 Antibody (A14648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-ihc-testing-5.jpgAnti-SFXN3 Antibody Picoband® IHC analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). SFXN3 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN3 Antibody (A14648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-ihc-testing-6.jpgAnti-SFXN3 Antibody Picoband® IHC analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). SFXN3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN3 Antibody (A14648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-ihc-testing-7.jpgAnti-SFXN3 Antibody Picoband® IHC analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). SFXN3 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN3 Antibody (A14648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-if-testing-8.jpgAnti-SFXN3 Antibody Picoband® IF analysis of SFXN3 using anti-SFXN3 antibody (A14648-1). SFXN3 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SFXN3 Antibody (A14648-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14648-1-sfxn3-primary-antibodies-fcm-testing-9.jpgAnti-SFXN3 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SFXN3 antibody (A14648-1). Overlay histogram showing A549 cells stained with A14648-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFXN3 Antibody (A14648-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sfxn5-picoband-trade-antibody-a15698-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-wb-testing-1.jpgAnti-SFXN5 Antibody Picoband® Western blot analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFXN5 antigen affinity purified polyclonal antibody (Catalog # A15698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFXN5 at approximately 37 kDa. The expected band size for SFXN5 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-2.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-3.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-4.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-5.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-6.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-7.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-8.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-9.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-10.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-ihc-testing-11.jpgAnti-SFXN5 Antibody Picoband® IHC analysis of SFXN5 using anti-SFXN5 antibody (A15698-1). SFXN5 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN5 Antibody (A15698-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-fcm-testing-12_1.jpgAnti-SFXN5 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SFXN5 antibody (A15698-1). Overlay histogram showing A549 cells stained with A15698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFXN5 Antibody (A15698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15698-1-sfxn5-primary-antibodies-fcm-testing-13.jpgAnti-SFXN5 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-SFXN5 antibody (A15698-1). Overlay histogram showing U937 cells stained with A15698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFXN5 Antibody (A15698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sgce-picoband-trade-antibody-a02507-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-wb-testing-1.jpgAnti-SGCE Antibody Picoband® Western blot analysis of SGCE using anti-SGCE antibody (A02507-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCE antigen affinity purified polyclonal antibody (Catalog # A02507-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGCE at approximately 50 kDa. The expected band size for SGCE is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-ihc-testing-2.jpgAnti-SGCE Antibody Picoband® IHC analysis of SGCE using anti-SGCE antibody (A02507-1). SGCE was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGCE Antibody (A02507-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-ihc-testing-3.jpgAnti-SGCE Antibody Picoband® IHC analysis of SGCE using anti-SGCE antibody (A02507-1). SGCE was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGCE Antibody (A02507-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-ihc-testing-4.jpgAnti-SGCE Antibody Picoband® IHC analysis of SGCE using anti-SGCE antibody (A02507-1). SGCE was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGCE Antibody (A02507-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-ihc-testing-5.jpgAnti-SGCE Antibody Picoband® IHC analysis of SGCE using anti-SGCE antibody (A02507-1). SGCE was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGCE Antibody (A02507-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-ihc-testing-6.jpgAnti-SGCE Antibody Picoband® IHC analysis of SGCE using anti-SGCE antibody (A02507-1). SGCE was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGCE Antibody (A02507-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-ihc-testing-7.jpgAnti-SGCE Antibody Picoband® IHC analysis of SGCE using anti-SGCE antibody (A02507-1). SGCE was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGCE Antibody (A02507-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-fcm-testing-8_1.jpgAnti-SGCE Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SGCE antibody (A02507-1). Overlay histogram showing A549 cells stained with A02507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGCE Antibody (A02507-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02507-1-sgce-primary-antibodies-fcm-testing-9.jpgAnti-SGCE Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SGCE antibody (A02507-1). Overlay histogram showing PC-3 cells stained with A02507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGCE Antibody (A02507-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sgo2-picoband-trade-antibody-a08632-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/A/0/A08632-SGO2-primary-antibodies-WB-testing-1.jpgAnti-SGO2 Antibody Picoband® Western blot analysis of SGO2 using anti-SGO2 antibody (A08632). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGO2 antigen affinity purified polyclonal antibody (Catalog # A08632) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGO2 at approximately 180 kDa. The expected band size for SGO2 is at 145 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08632-sgo2-primary-antibodies-if-testing-2.jpgAnti-SGO2 Antibody Picoband® IF analysis of SGO2 and Tubulin alpha using anti-SGO2 antibody (A08632) and anti-Tubulin alpha antibody (M03989-3). SGO2 and Tubulin alpha were detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SGO2 antibody (A08632) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08632-sgo2-primary-antibodies-fcm-testing-3.jpgAnti-SGO2 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-SGO2 antibody (A08632). Overlay histogram showing CACO-2 cells stained with A08632 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGO2 Antibody (A08632, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08632-sgo2-primary-antibodies-fcm-testing-4.jpgAnti-SGO2 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-SGO2 antibody (A08632). Overlay histogram showing RT4 cells stained with A08632 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGO2 Antibody (A08632, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hss-sgsh-picoband-trade-antibody-a04608-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04608-1-sgsh-primary-antibodies-wb-testing-1_1.jpgAnti-HSS/SGSH Antibody Picoband®https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04608-1-sgsh-primary-antibodies-ihc-testing-1.jpgAnti-HSS/SGSH Antibody Picoband®IHC analysis of HSS/SGSH using anti-HSS/SGSH antibody (A04608-1). HSS/SGSH was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSS/SGSH Antibody (A04608-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04608-1-sgsh-primary-antibodies-ihc-testing-2.jpgAnti-HSS/SGSH Antibody Picoband®IHC analysis of HSS/SGSH using anti-HSS/SGSH antibody (A04608-1). HSS/SGSH was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSS/SGSH Antibody (A04608-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04608-1-sgsh-primary-antibodies-ihc-testing-3.jpgAnti-HSS/SGSH Antibody Picoband®IHC analysis of HSS/SGSH using anti-HSS/SGSH antibody (A04608-1). HSS/SGSH was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSS/SGSH Antibody (A04608-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04608-1-sgsh-primary-antibodies-ihc-testing-4.jpgAnti-HSS/SGSH Antibody Picoband®IHC analysis of HSS/SGSH using anti-HSS/SGSH antibody (A04608-1). HSS/SGSH was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSS/SGSH Antibody (A04608-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04608-1-sgsh-primary-antibodies-ihc-testing-5.jpgAnti-HSS/SGSH Antibody Picoband®IHC analysis of HSS/SGSH using anti-HSS/SGSH antibody (A04608-1). HSS/SGSH was detected in a paraffin-embedded section of human urothelium carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSS/SGSH Antibody (A04608-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04608-1-sgsh-primary-antibodies-fcm-testing-1.jpgAnti-HSS/SGSH Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-HSS/SGSH antibody (A04608-1). Overlay histogram showing MCF-7 cells stained with A04608-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSS/SGSH Antibody (A04608-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sgta-picoband-trade-antibody-a03818-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03818-1-sgta-primary-antibodies-wb-testing-1_1.jpgAnti-SGTA Antibody Picoband® Western blot analysis of SGTA using anti-SGTA antibody (A03818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGTA antigen affinity purified polyclonal antibody (Catalog # A03818-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGTA at approximately 37 kDa. The expected band size for SGTA is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03818-1-sgta-primary-antibodies-if-testing-2.jpgAnti-SGTA Antibody Picoband® IF analysis of SGTA and Tubulin alpha using anti-SGTA antibody (A03818-1) and anti-Tubulin alpha antibody (M03989-3). SGTA and Tubulin alpha were detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SGTA antibody (A03818-1) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03818-1-sgta-primary-antibodies-fcm-testing-3.jpgAnti-SGTA Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SGTA antibody (A03818-1). Overlay histogram showing Jurkat cells stained with A03818-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGTA Antibody (A03818-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sgt2-sgtb-picoband-trade-antibody-a13056-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13056-1-sgtb-primary-antibodies-wb-testing-1.jpgAnti-SGT2/SGTB Antibody Picoband® Western blot analysis of SGT2/SGTB using anti-SGT2/SGTB antibody (A13056-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGT2/SGTB antigen affinity purified polyclonal antibody (Catalog # A13056-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGT2/SGTB at approximately 36 kDa. The expected band size for SGT2/SGTB is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sh2d4a-picoband-trade-antibody-a09094-2-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-wb-testing-1.jpgAnti-SH2D4A Antibody Picoband® Western blot analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2D4A antigen affinity purified polyclonal antibody (Catalog # A09094-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH2D4A at approximately 50 kDa. The expected band size for SH2D4A is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-2.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-3.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-4.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-5.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-6.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-7.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-8.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-9.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-ihc-testing-10.jpgAnti-SH2D4A Antibody Picoband® IHC analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in a paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-if-testing-11.jpgAnti-SH2D4A Antibody Picoband® IF analysis of SH2D4A using anti-SH2D4A antibody (A09094-2). SH2D4A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SH2D4A Antibody (A09094-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09094-2-sh2d4a-primary-antibodies-fcm-testing-12_1.jpgAnti-SH2D4A Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SH2D4A antibody (A09094-2). Overlay histogram showing A549 cells stained with A09094-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH2D4A Antibody (A09094-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sh3gl3-picoband-trade-antibody-a09836-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09836-1-sh3gl3-primary-antibodies-wb-testing-1.jpgAnti-SH3GL3 Antibody Picoband® Western blot analysis of SH3GL3 using anti-SH3GL3 antibody (A09836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3GL3 antigen affinity purified polyclonal antibody (Catalog # A09836-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3GL3 at approximately 42 kDa. The expected band size for SH3GL3 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09836-1-sh3gl3-primary-antibodies-fcm-testing-2.jpgAnti-SH3GL3 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SH3GL3 antibody (A09836-1). Overlay histogram showing Jurkat cells stained with A09836-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH3GL3 Antibody (A09836-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09836-1-sh3gl3-primary-antibodies-fcm-testing-3.jpgAnti-SH3GL3 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SH3GL3 antibody (A09836-1). Overlay histogram showing U20S cells stained with A09836-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH3GL3 Antibody (A09836-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-shcbp1l-picoband-trade-antibody-a15056-2-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15056-2-shcbp1l-primary-antibodies-wb-testing-1.jpgAnti-SHCBP1L Antibody Picoband® Western blot analysis of SHCBP1L using anti-SHCBP1L antibody (A15056-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHCBP1L antigen affinity purified polyclonal antibody (Catalog # A15056-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHCBP1L at approximately 90 kDa. The expected band size for SHCBP1L is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-shq1-picoband-trade-antibody-a10185-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-wb-testing-1.jpgAnti-SHQ1 Antibody Picoband® Western blot analysis of SHQ1 using anti-SHQ1 antibody (A10185-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHQ1 antigen affinity purified polyclonal antibody (Catalog # A10185-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHQ1 at approximately 80 kDa. The expected band size for SHQ1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-ihc-testing-2.jpgAnti-SHQ1 Antibody Picoband® IHC analysis of SHQ1 using anti-SHQ1 antibody (A10185-1). SHQ1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHQ1 Antibody (A10185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-ihc-testing-3.jpgAnti-SHQ1 Antibody Picoband® IHC analysis of SHQ1 using anti-SHQ1 antibody (A10185-1). SHQ1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHQ1 Antibody (A10185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-ihc-testing-4.jpgAnti-SHQ1 Antibody Picoband® IHC analysis of SHQ1 using anti-SHQ1 antibody (A10185-1). SHQ1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHQ1 Antibody (A10185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-ihc-testing-5.jpgAnti-SHQ1 Antibody Picoband® IHC analysis of SHQ1 using anti-SHQ1 antibody (A10185-1). SHQ1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHQ1 Antibody (A10185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-ihc-testing-6.jpgAnti-SHQ1 Antibody Picoband® IHC analysis of SHQ1 using anti-SHQ1 antibody (A10185-1). SHQ1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHQ1 Antibody (A10185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-if-testing-7_1.jpgAnti-SHQ1 Antibody Picoband® IF analysis of SHQ1 using anti-SHQ1 antibody (A10185-1). SHQ1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SHQ1 Antibody (A10185-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10185-1-shq1-primary-antibodies-fcm-testing-8.jpgAnti-SHQ1 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-SHQ1 antibody (A10185-1). Overlay histogram showing A549 cells stained with A10185-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHQ1 Antibody (A10185-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-shroom1-picoband-trade-antibody-a12817-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12817-1-shroom1-primary-antibodies-wb-testing-1.jpgAnti-SHROOM1 Antibody Picoband® Western blot analysis of SHROOM1 using anti-SHROOM1 antibody (A12817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM1 antigen affinity purified polyclonal antibody (Catalog # A12817-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM1 at approximately 91 kDa. The expected band size for SHROOM1 is at 91 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-shroom2-picoband-trade-antibody-a10015-2-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10015-2-shroom2-primary-antibodies-wb-testing-1.jpgAnti-SHROOM2 Antibody Picoband® Western blot analysis of SHROOM2 using anti-SHROOM2 antibody (A10015-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM2 antigen affinity purified polyclonal antibody (Catalog # A10015-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM2 at approximately 180 kDa. The expected band size for SHROOM2 is at 176 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10015-2-shroom2-primary-antibodies-fcm-testing-2.jpgAnti-SHROOM2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SHROOM2 antibody (A10015-2). Overlay histogram showing U20S cells stained with A10015-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHROOM2 Antibody (A10015-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-shroom4-picoband-trade-antibody-a10866-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10866-shroom4-primary-antibodies-wb-testing-1.jpgAnti-SHROOM4 Antibody Picoband® Western blot analysis of SHROOM4 using anti-SHROOM4 antibody (A10866). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human U-87 MG whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM4 antigen affinity purified polyclonal antibody (Catalog # A10866) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM4 at approximately 170-180 kDa. The expected band size for SHROOM4 is at 165 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10866-shroom4-primary-antibodies-fcm-testing-2.jpgAnti-SHROOM4 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-SHROOM4 antibody (A10866). Overlay histogram showing RT4 cells stained with A10866 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHROOM4 Antibody (A10866, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10866-shroom4-primary-antibodies-fcm-testing-3.jpgAnti-SHROOM4 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SHROOM4 antibody (A10866). Overlay histogram showing U20S cells stained with A10866 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHROOM4 Antibody (A10866, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-msin3a-sin3a-picoband-trade-antibody-a01203-2-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-wb-testing-1.jpgAnti-mSin3A/SIN3A Antibody Picoband® Western blot analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mSin3A/SIN3A antigen affinity purified polyclonal antibody (Catalog # A01203-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mSin3A/SIN3A at approximately 150 kDa. The expected band size for mSin3A/SIN3A is at 145 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-ihc-testing-2.jpgAnti-mSin3A/SIN3A Antibody Picoband® IHC analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). mSin3A/SIN3A was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mSin3A/SIN3A Antibody (A01203-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-ihc-testing-3.jpgAnti-mSin3A/SIN3A Antibody Picoband® IHC analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). mSin3A/SIN3A was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mSin3A/SIN3A Antibody (A01203-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-ihc-testing-4.jpgAnti-mSin3A/SIN3A Antibody Picoband® IHC analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). mSin3A/SIN3A was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mSin3A/SIN3A Antibody (A01203-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-ihc-testing-5.jpgAnti-mSin3A/SIN3A Antibody Picoband® IHC analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). mSin3A/SIN3A was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mSin3A/SIN3A Antibody (A01203-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-ihc-testing-6.jpgAnti-mSin3A/SIN3A Antibody Picoband® IHC analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). mSin3A/SIN3A was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mSin3A/SIN3A Antibody (A01203-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-if-testing-7.jpgAnti-mSin3A/SIN3A Antibody Picoband® IF analysis of mSin3A/SIN3A and Tubulin alpha using anti-mSin3A/SIN3A antibody (A01203-2) and anti-Tubulin alpha antibody (M03989-3). mSin3A/SIN3A and Tubulin alpha were detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-mSin3A/SIN3A antibody (A01203-2) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-fcm-testing-9_1.jpgAnti-mSin3A/SIN3A Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-mSin3A/SIN3A antibody (A01203-2). Overlay histogram showing 293T cells stained with A01203-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-mSin3A/SIN3A Antibody (A01203-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-if-testing-8.jpgAnti-mSin3A/SIN3A Antibody Picoband® IF analysis of mSin3A/SIN3A using anti-mSin3A/SIN3A antibody (A01203-2). mSin3A/SIN3A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-mSin3A/SIN3A Antibody (A01203-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01203-2-sin3a-primary-antibodies-fcm-testing-10.jpgAnti-mSin3A/SIN3A Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-mSin3A/SIN3A antibody (A01203-2). Overlay histogram showing SiHa cells stained with A01203-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-mSin3A/SIN3A Antibody (A01203-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sinhcaf-picoband-trade-antibody-a31944-1-boster.html2026-03-17T05:14:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-wb-testing-1.jpgAnti-SINHCAF Antibody Picoband® Western blot analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SINHCAF antigen affinity purified polyclonal antibody (Catalog # A31944-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SINHCAF at approximately 28-30 kDa. The expected band size for SINHCAF is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-2.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-3.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-4.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-5.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-6.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-7.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-8.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-ihc-testing-9.jpgAnti-SINHCAF Antibody Picoband® IHC analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-if-testing-10.jpgAnti-SINHCAF Antibody Picoband® IF analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). SINHCAF was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SINHCAF Antibody (A31944-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31944-1-sinhcaf-primary-antibodies-fcm-testing-11.jpgAnti-SINHCAF Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-SINHCAF antibody (A31944-1). Overlay histogram showing U937 cells stained with A31944-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SINHCAF Antibody (A31944-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-siva-siva1-picoband-trade-antibody-a05419-1-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05419-1-siva1-primary-antibodies-wb-testing-1.jpgAnti-SIVA/SIVA1 Antibody Picoband® Western blot analysis of SIVA/SIVA1 using anti-SIVA/SIVA1 antibody (A05419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIVA/SIVA1 antigen affinity purified polyclonal antibody (Catalog # A05419-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIVA/SIVA1 at approximately 19 kDa. The expected band size for SIVA/SIVA1 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05419-1-siva1-primary-antibodies-fcm-testing-2_1.jpgAnti-SIVA/SIVA1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-SIVA/SIVA1 antibody (A05419-1). Overlay histogram showing U937 cells stained with A05419-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIVA/SIVA1 Antibody (A05419-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05419-1-siva1-primary-antibodies-fcm-testing-3.jpgAnti-SIVA/SIVA1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SIVA/SIVA1 antibody (A05419-1). Overlay histogram showing PC-3 cells stained with A05419-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIVA/SIVA1 Antibody (A05419-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc5a4-picoband-trade-antibody-a15467-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15467-2-slc5a4-primary-antibodies-wb-testing-1.jpgAnti-SLC5A4 Antibody Picoband® Western blot analysis of SLC5A4 using anti-SLC5A4 antibody (A15467-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A4 antigen affinity purified polyclonal antibody (Catalog # A15467-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A4 at approximately 80 kDa. The expected band size for SLC5A4 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15467-2-slc5a4-primary-antibodies-fcm-testing-2.jpgAnti-SLC5A4 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SLC5A4 antibody (A15467-2). Overlay histogram showing 293T cells stained with A15467-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A4 Antibody (A15467-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc6a17-picoband-trade-antibody-a12725-1-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12725-1-slc6a17-primary-antibodies-wb-testing-1.jpgAnti-SLC6A17 Antibody Picoband® Western blot analysis of SLC6A17 using anti-SLC6A17 antibody (A12725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A17 antigen affinity purified polyclonal antibody (Catalog # A12725-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A17 at approximately 81 kDa. The expected band size for SLC6A17 is at 81 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc6a18-picoband-trade-antibody-a10812-1-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10812-1-slc6a18-primary-antibodies-wb-testing-1.jpgAnti-SLC6A18 Antibody Picoband® Western blot analysis of SLC6A18 using anti-SLC6A18 antibody (A10812-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A18 antigen affinity purified polyclonal antibody (Catalog # A10812-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A18 at approximately 71 kDa. The expected band size for SLC6A18 is at 71 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-nherf-2-sip-1-slc9a3r2-picoband-trade-antibody-a05624-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-wb-testing-1.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® Western blot analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A3R2 antigen affinity purified polyclonal antibody (Catalog # A05624-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A3R2 at approximately 45 kDa. The expected band size for SLC9A3R2 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-2.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-3.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-4.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-5.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-6.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-7.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-8.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-9.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of rat skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-ihc-testing-10.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® IHC analysis of SLC9A3R2 using anti-SLC9A3R2 antibody (A05624-2). SLC9A3R2 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A3R2 Antibody (A05624-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05624-2-slc9a3r2-primary-antibodies-fcm-testing-11.jpgAnti-NHERF-2/SIP-1/SLC9A3R2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SLC9A3R2 antibody (A05624-2). Overlay histogram showing PC-3 cells stained with A05624-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC9A3R2 Antibody (A05624-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc17a5-picoband-trade-antibody-a06657-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06657-2-slc17a5-primary-antibodies-wb-testing-1.jpgAnti-SLC17A5 Antibody Picoband® Western blot analysis of SLC17A5 using anti-SLC17A5 antibody (A06657-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse HBZY-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC17A5 antigen affinity purified polyclonal antibody (Catalog # A06657-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC17A5 at approximately 36 kDa. The expected band size for SLC17A5 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06657-2-slc17a5-primary-antibodies-ihc-testing-2.jpgAnti-SLC17A5 Antibody Picoband® IHC analysis of SLC17A5 using anti-SLC17A5 antibody (A06657-2). SLC17A5 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC17A5 Antibody (A06657-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-oat3-slc22a8-picoband-trade-antibody-a04087-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04087-2-slc22a8-primary-antibodies-wb-testing-1.jpgAnti-OAT3/SLC22A8 Antibody Picoband® Western blot analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT3/SLC22A8 antigen affinity purified polyclonal antibody (Catalog # A04087-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT3/SLC22A8 at approximately 62 kDa. The expected band size for OAT3/SLC22A8 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04087-2-slc22a8-primary-antibodies-ihc-testing-2.jpgAnti-OAT3/SLC22A8 Antibody Picoband® IHC analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). OAT3/SLC22A8 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT3/SLC22A8 Antibody (A04087-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04087-2-slc22a8-primary-antibodies-ihc-testing-3.jpgAnti-OAT3/SLC22A8 Antibody Picoband® IHC analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). OAT3/SLC22A8 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT3/SLC22A8 Antibody (A04087-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04087-2-slc22a8-primary-antibodies-ihc-testing-4.jpgAnti-OAT3/SLC22A8 Antibody Picoband® IHC analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). OAT3/SLC22A8 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT3/SLC22A8 Antibody (A04087-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04087-2-slc22a8-primary-antibodies-ihc-testing-5.jpgAnti-OAT3/SLC22A8 Antibody Picoband® IHC analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). OAT3/SLC22A8 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT3/SLC22A8 Antibody (A04087-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04087-2-slc22a8-primary-antibodies-if-testing-6.jpgAnti-OAT3/SLC22A8 Antibody Picoband® IF analysis of OAT3/SLC22A8 using anti-OAT3/SLC22A8 antibody (A04087-2). OAT3/SLC22A8 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-OAT3/SLC22A8 Antibody (A04087-2) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04087-2-slc22a8-primary-antibodies-fcm-testing-7.jpgAnti-OAT3/SLC22A8 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-OAT3/SLC22A8 antibody (A04087-2). Overlay histogram showing K562 cells stained with A04087-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OAT3/SLC22A8 Antibody (A04087-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bal-slc27a5-picoband-trade-antibody-a09287-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09287-2-slc27a5-primary-antibodies-wb-testing-1.jpgAnti-BAL/SLC27A5 Antibody Picoband® Western blot analysis of BAL/SLC27A5 using anti-BAL/SLC27A5 antibody (A09287-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAL/SLC27A5 antigen affinity purified polyclonal antibody (Catalog # A09287-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAL/SLC27A5 at approximately 75 kDa. The expected band size for BAL/SLC27A5 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09287-2-slc27a5-primary-antibodies-fcm-testing-2.jpgAnti-BAL/SLC27A5 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-BAL/SLC27A5 antibody (A09287-2). Overlay histogram showing Jurkat cells stained with A09287-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAL/SLC27A5 Antibody (A09287-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc40a1-picoband-trade-antibody-a01953-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-wb-testing-1.jpgAnti-SLC40A1 Antibody Picoband® Western blot analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC40A1 antigen affinity purified polyclonal antibody (Catalog # A01953-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC40A1 at approximately 70 kDa. The expected band size for SLC40A1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-41598_2023_49697_fig4_html.pngAnti-SLC40A1 Antibody Picoband®FSGS rats showed iron metabolism disorder. ( A ) Prussian blue staining (scale bar: 100 μm). ( B ) Fe 2+ results. ( C ) Western blot analysis of hepcidin, ferroportin and TFR. ( D ) Relative expression of hepcidin. ( E ) Relative expression of ferroportin. ( F ) Relative expression of TFR. * P < 0.05. Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-023-49697-8'>38097813</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-ihc-testing-2.jpgAnti-SLC40A1 Antibody Picoband® IHC analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). SLC40A1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC40A1 Antibody (A01953-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-ihc-testing-3.jpgAnti-SLC40A1 Antibody Picoband® IHC analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). SLC40A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC40A1 Antibody (A01953-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-ihc-testing-4.jpgAnti-SLC40A1 Antibody Picoband® IHC analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). SLC40A1 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC40A1 Antibody (A01953-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-ihc-testing-5.jpgAnti-SLC40A1 Antibody Picoband® IHC analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). SLC40A1 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC40A1 Antibody (A01953-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-ihc-testing-6.jpgAnti-SLC40A1 Antibody Picoband® IHC analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). SLC40A1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC40A1 Antibody (A01953-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-if-testing-7.jpgAnti-SLC40A1 Antibody Picoband® IF analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). SLC40A1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC40A1 Antibody (A01953-2) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-slc40a1-primary-antibodies-fcm-testing-8.jpgAnti-SLC40A1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SLC40A1 antibody (A01953-2). Overlay histogram showing U20S cells stained with A01953-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC40A1 Antibody (A01953-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01953-2-anfeng_ning.pngAnti-SLC40A1 Antibody Picoband®Western blot analysis of SLC40A1 using anti-SLC40A1 antibody (A04887-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1-5: model group-human uterine tissue lysates, Lane 6-10: young group-human uterine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC40A1 antigen affinity purified polyclonal antibody (A04887-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system. A specific band was detected for SLC40A1 at approximately 60-70 kDa. The expected band size for SLC40A1 is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-snf5-smarcb1-picoband-trade-antibody-a00500-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00500-2-smarcb1-primary-antibodies-wb-testing-1.jpgAnti-SNF5/SMARCB1 Antibody Picoband® Western blot analysis of SNF5/SMARCB1 using anti-SNF5/SMARCB1 antibody (A00500-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF5/SMARCB1 antigen affinity purified polyclonal antibody (Catalog # A00500-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF5/SMARCB1 at approximately 44 kDa. The expected band size for SNF5/SMARCB1 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00500-2-smarcb1-primary-antibodies-fcm-testing-2.jpgAnti-SNF5/SMARCB1 Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-SNF5/SMARCB1 antibody (A00500-2). Overlay histogram showing C6 cells stained with A00500-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNF5/SMARCB1 Antibody (A00500-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-baf57-smarce1-picoband-trade-antibody-a03739-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03739-2-smarce1-primary-antibodies-wb-testing-1.jpgAnti-BAF57/SMARCE1 Antibody Picoband® Western blot analysis of BAF57/SMARCE1 using anti-BAF57/SMARCE1 antibody (A03739-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAF57/SMARCE1 antigen affinity purified polyclonal antibody (Catalog # A03739-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAF57/SMARCE1 at approximately 54 kDa. The expected band size for BAF57/SMARCE1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03739-2-smarce1-primary-antibodies-ihc-testing-2.jpgAnti-BAF57/SMARCE1 Antibody Picoband® IHC analysis of BAF57/SMARCE1 using anti-BAF57/SMARCE1 antibody (A03739-2). BAF57/SMARCE1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAF57/SMARCE1 Antibody (A03739-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03739-2-smarce1-primary-antibodies-ihc-testing-3.jpgAnti-BAF57/SMARCE1 Antibody Picoband® IHC analysis of BAF57/SMARCE1 using anti-BAF57/SMARCE1 antibody (A03739-2). BAF57/SMARCE1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAF57/SMARCE1 Antibody (A03739-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03739-2-smarce1-primary-antibodies-if-testing-4.jpgAnti-BAF57/SMARCE1 Antibody Picoband® IF analysis of BAF57/SMARCE1 using anti-BAF57/SMARCE1 antibody (A03739-2). BAF57/SMARCE1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BAF57/SMARCE1 Antibody (A03739-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03739-2-smarce1-primary-antibodies-fcm-testing-5.jpgAnti-BAF57/SMARCE1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-BAF57/SMARCE1 antibody (A03739-2). Overlay histogram showing PC-3 cells stained with A03739-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAF57/SMARCE1 Antibody (A03739-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spop-picoband-trade-antibody-a02032-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02032-2-spop-primary-antibodies-wb-testing-1.jpgAnti-SPOP Antibody Picoband® Western blot analysis of SPOP using anti-SPOP antibody (A02032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOP antigen affinity purified polyclonal antibody (Catalog # A02032-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPOP at approximately 50 kDa. The expected band size for SPOP is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-thymidine-kinase-2-tk2-picoband-trade-antibody-a03321-1-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03321-1-tk2-primary-antibodies-wb-testing-1.jpgAnti-Thymidine Kinase 2/TK2 Antibody Picoband® Western blot analysis of Thymidine Kinase 2/TK2 using anti-Thymidine Kinase 2/TK2 antibody (A03321-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thymidine Kinase 2/TK2 antigen affinity purified polyclonal antibody (Catalog # A03321-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thymidine Kinase 2/TK2 at approximately 25 kDa. The expected band size for Thymidine Kinase 2/TK2 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03321-1-tk2-primary-antibodies-fcm-testing-2.jpgAnti-Thymidine Kinase 2/TK2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-Thymidine Kinase 2/TK2 antibody (A03321-1). Overlay histogram showing Jurkat cells stained with A03321-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thymidine Kinase 2/TK2 Antibody (A03321-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tweak-tnfsf12-picoband-trade-antibody-a02009-3-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02009-3-tnfsf12-primary-antibodies-wb-testing-1.jpgAnti-TWEAK/Tnfsf12 Antibody Picoband® Western blot analysis of TWEAK/Tnfsf12 using anti-TWEAK/Tnfsf12 antibody (A02009-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWEAK/Tnfsf12 antigen affinity purified polyclonal antibody (Catalog # A02009-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWEAK/Tnfsf12 at approximately 27 kDa. The expected band size for TWEAK/Tnfsf12 is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pig3-tp53i3-picoband-trade-antibody-a06870-3-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06870-3-tp53i3-primary-antibodies-wb-testing-1.jpgAnti-PIG3/TP53I3 Antibody Picoband® Western blot analysis of PIG3/TP53I3 using anti-PIG3/TP53I3 antibody (A06870-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIG3/TP53I3 antigen affinity purified polyclonal antibody (Catalog # A06870-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIG3/TP53I3 at approximately 36 kDa. The expected band size for PIG3/TP53I3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06870-3-tp53i3-primary-antibodies-ihc-testing-2.jpgAnti-PIG3/TP53I3 Antibody Picoband® IHC analysis of PIG3/TP53I3 using anti-PIG3/TP53I3 antibody (A06870-3). PIG3/TP53I3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIG3/TP53I3 Antibody (A06870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06870-3-tp53i3-primary-antibodies-ihc-testing-3.jpgAnti-PIG3/TP53I3 Antibody Picoband® IHC analysis of PIG3/TP53I3 using anti-PIG3/TP53I3 antibody (A06870-3). PIG3/TP53I3 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIG3/TP53I3 Antibody (A06870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06870-3-tp53i3-primary-antibodies-ihc-testing-4.jpgAnti-PIG3/TP53I3 Antibody Picoband® IHC analysis of PIG3/TP53I3 using anti-PIG3/TP53I3 antibody (A06870-3). PIG3/TP53I3 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIG3/TP53I3 Antibody (A06870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06870-3-tp53i3-primary-antibodies-ihc-testing-5.jpgAnti-PIG3/TP53I3 Antibody Picoband® IHC analysis of PIG3/TP53I3 using anti-PIG3/TP53I3 antibody (A06870-3). PIG3/TP53I3 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIG3/TP53I3 Antibody (A06870-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-trmt61a-picoband-trade-antibody-a14512-1-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-wb-testing-1.jpgAnti-TRMT61A Antibody Picoband® Western blot analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT61A antigen affinity purified polyclonal antibody (Catalog # A14512-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT61A at approximately 31 kDa. The expected band size for TRMT61A is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-2.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-3.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-4.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-5.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-6.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human lung adenocarcinom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-7.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-8.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-9.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-10.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-11.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-12.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-13.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-14.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-15.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-16.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-17.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of rat skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-ihc-testing-18.jpgAnti-TRMT61A Antibody Picoband® IHC analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-if-testing-19.jpgAnti-TRMT61A Antibody Picoband® IF analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). TRMT61A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRMT61A Antibody (A14512-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-fcm-testing-20.jpgAnti-TRMT61A Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-TRMT61A antibody (A14512-1). Overlay histogram showing PC-3 cells stained with A14512-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT61A Antibody (A14512-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14512-1-trmt61a-primary-antibodies-fcm-testing-21.jpgAnti-TRMT61A Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-TRMT61A antibody (A14512-1). Overlay histogram showing HepG2 cells stained with A14512-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT61A Antibody (A14512-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tsnaxip1-picoband-trade-antibody-a18904-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18904-tsnaxip1-primary-antibodies-wb-testing-1.jpgAnti-TSNAXIP1 Antibody Picoband® Western blot analysis of TSNAXIP1 using anti-TSNAXIP1 antibody (A18904). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSNAXIP1 antigen affinity purified polyclonal antibody (Catalog # A18904) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSNAXIP1 at approximately 70 kDa, 50 kDa. The expected band size for TSNAXIP1 is at 77 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tubg1-2-picoband-trade-antibody-a06313-2-boster.html2026-03-17T05:14:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06313-2-tubg1-2-primary-antibodies-wb-testing-1.jpgAnti-TUBG1/2 Antibody Picoband® Western blot analysis of TUBG1/2 using anti-TUBG1/2 antibody (A06313-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBG1/2 antigen affinity purified polyclonal antibody (Catalog # A06313-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBG1/2 at approximately 51 kDa. The expected band size for TUBG1/2 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06313-2-tubg1-2-primary-antibodies-wb-testing-2.jpgAnti-TUBG1/2 Antibody Picoband® Western blot analysis of TUBG1/2 using anti-TUBG1/2 antibody (A06313-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBG1/2 antigen affinity purified polyclonal antibody (Catalog # A06313-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBG1/2 at approximately 51 kDa. The expected band size for TUBG1/2 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tulp3-picoband-trade-antibody-a09384-2-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09384-2-tulp3-primary-antibodies-wb-testing-1_1.jpgAnti-TULP3 Antibody Picoband®Western blot analysis of TULP3 using anti-TULP3 antibody (A09384-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TULP3 antigen affinity purified polyclonal antibody (Catalog # A09384-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TULP3 at approximately 60 kDa. The expected band size for TULP3 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09384-2-tulp3-primary-antibodies-if-testing-1.jpgAnti-TULP3 Antibody Picoband®IF analysis of TULP3 using anti-TULP3 antibody (A09384-2) and anti-Tubulin Alpha antibody (M03989-3). TULP3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TULP3 Antibody (A09384-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09384-2-tulp3-primary-antibodies-fcm-testing-1.jpgAnti-TULP3 Antibody Picoband®Flow Cytometry analysis of SiHa cells using anti-TULP3 antibody (A09384-2). Overlay histogram showing SiHa cells stained with A09384-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TULP3 Antibody (A09384-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-yb1-ybx1-picoband-trade-antibody-a01054-1-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01054-1-ybx1-primary-antibodies-wb-testing-1.jpgAnti-YB1/Ybx1 Antibody Picoband® Western blot analysis of YB1/Ybx1 using anti-YB1/Ybx1 antibody (A01054-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat RH-35 whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YB1/Ybx1 antigen affinity purified polyclonal antibody (Catalog # A01054-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YB1/Ybx1 at approximately 50 kDa. The expected band size for YB1/Ybx1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01054-1-ybx1-primary-antibodies-fcm-testing-2.jpgAnti-YB1/Ybx1 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-YB1/Ybx1 antibody (A01054-1). Overlay histogram showing ANA-1 cells stained with A01054-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YB1/Ybx1 Antibody (A01054-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pin1-picoband-trade-antibody-m00467-1-boster.html2026-03-25T05:24:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00467-1-pin1-primary-antibodies-wb-testing-1.jpgAnti-Pin1 Antibody Picoband® (monoclonal, 5E5) Western blot analysis of Pin1 using anti-Pin1 antibody (M00467-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Pin1 antigen affinity purified monoclonal antibody (Catalog # M00467-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Pin1 at approximately 18 kDa. The expected band size for Pin1 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00467-1-pin1-primary-antibodies-if-testing-2.jpgAnti-Pin1 Antibody Picoband® (monoclonal, 5E5) IF analysis of Pin1 using anti-Pin1 antibody (M00467-1). Pin1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Pin1 Antibody (M00467-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00467-1-pin1-primary-antibodies-fcm-testing-3.jpgAnti-Pin1 Antibody Picoband® (monoclonal, 5E5) Flow Cytometry analysis of U87 cells using anti-Pin1 antibody (M00467-1). Overlay histogram showing U87 cells stained with M00467-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Pin1 Antibody (M00467-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-park7-dj1-picoband-trade-antibody-m00757-4-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00757-4-dj1-primary-antibodies-wb-testing-1.jpgAnti-PARK7/DJ1 Antibody Picoband® (monoclonal, 4B10) Western blot analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody (M00757-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PARK7/DJ1 antigen affinity purified monoclonal antibody (Catalog # M00757-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PARK7/DJ1 at approximately 22 kDa. The expected band size for PARK7/DJ1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00757-4-dj1-primary-antibodies-ihc-testing-2.jpgAnti-PARK7/DJ1 Antibody Picoband® (monoclonal, 4B10) IHC analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody (M00757-4). PARK7/DJ1 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PARK7/DJ1 Antibody (M00757-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00757-4-dj1-primary-antibodies-ihc-testing-3.jpgAnti-PARK7/DJ1 Antibody Picoband® (monoclonal, 4B10) IHC analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody (M00757-4). PARK7/DJ1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PARK7/DJ1 Antibody (M00757-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00757-4-dj1-primary-antibodies-ihc-testing-4.jpgAnti-PARK7/DJ1 Antibody Picoband® (monoclonal, 4B10) IHC analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody (M00757-4). PARK7/DJ1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-PARK7/DJ1 Antibody (M00757-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00757-4-dj1-primary-antibodies-if-testing-5.jpgAnti-PARK7/DJ1 Antibody Picoband® (monoclonal, 4B10) IF analysis of PARK7/DJ1 using anti-PARK7/DJ1 antibody (M00757-4). PARK7/DJ1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-PARK7/DJ1 Antibody (M00757-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00757-4-dj1-primary-antibodies-fcm-testing-6.jpgAnti-PARK7/DJ1 Antibody Picoband® (monoclonal, 4B10) Flow Cytometry analysis of Hela cells using anti-PARK7/DJ1 antibody (M00757-4). Overlay histogram showing Hela cells stained with M00757-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PARK7/DJ1 Antibody (M00757-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ikaros-ikzf1-picoband-trade-antibody-m00531-3-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00531-3-ikzf1-primary-antibodies-wb-testing-1.jpgAnti-Ikaros/IKZF1 Antibody Picoband® (monoclonal, 5F12H7) Western blot analysis of Ikaros/IKZF1 using anti-Ikaros/IKZF1 antibody (M00531-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Ikaros/IKZF1 antigen affinity purified monoclonal antibody (Catalog # M00531-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Ikaros/IKZF1 at approximately 55-65 kDa. The expected band size for Ikaros/IKZF1 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ifi16-picoband-trade-antibody-m00848-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00848-ifi16-primary-antibodies-wb-testing-1.jpgAnti-IFI16 Antibody Picoband® (monoclonal, 2I3D7) Western blot analysis of IFI16 using anti-IFI16 antibody (M00848). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human U-87 MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IFI16 antigen affinity purified monoclonal antibody (Catalog # M00848) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IFI16 at approximately 98 kDa. The expected band size for IFI16 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00848-ifi16-primary-antibodies-if-testing-2.jpgAnti-IFI16 Antibody Picoband® (monoclonal, 2I3D7) IF analysis of IFI16 using anti-IFI16 antibody (M00848). IFI16 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-IFI16 Antibody (M00848) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00848-ifi16-primary-antibodies-fcm-testing-3.jpgAnti-IFI16 Antibody Picoband® (monoclonal, 2I3D7) Flow Cytometry analysis of U251 cells using anti-IFI16 antibody (M00848). Overlay histogram showing U251 cells stained with M00848 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IFI16 Antibody (M00848, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irbit-ahcyl1-picoband-trade-antibody-m08908-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08908-ahcyl1-primary-antibodies-wb-testing-1.jpgAnti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9) Western blot analysis of IRBIT/AHCYL1 using anti-IRBIT/AHCYL1 antibody (M08908). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-IRBIT/AHCYL1 antigen affinity purified monoclonal antibody (Catalog # M08908) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IRBIT/AHCYL1 at approximately 59 kDa. The expected band size for IRBIT/AHCYL1 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08908-ahcyl1-primary-antibodies-fcm-testing-2.jpgAnti-IRBIT/AHCYL1 Antibody Picoband® (monoclonal, 2E7D9) Flow Cytometry analysis of K562 cells using anti-IRBIT/AHCYL1 antibody (M08908). Overlay histogram showing K562 cells stained with M08908 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IRBIT/AHCYL1 Antibody (M08908, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-golgin-97-golga1-picoband-trade-antibody-m13524-1-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13524-1-golga1-primary-antibodies-wb-testing-1.jpgAnti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1) Western blot analysis of Golgin 97/GOLGA1 using anti-Golgin 97/GOLGA1 antibody (M13524-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Golgin 97/GOLGA1 antigen affinity purified monoclonal antibody (Catalog # M13524-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Golgin 97/GOLGA1 at approximately 97 kDa. The expected band size for Golgin 97/GOLGA1 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13524-1-golga1-primary-antibodies-if-testing-2.jpgAnti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1) IF analysis of Golgin 97/GOLGA1 using anti-Golgin 97/GOLGA1 antibody (M13524-1). Golgin 97/GOLGA1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Golgin 97/GOLGA1 Antibody (M13524-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13524-1-golga1-primary-antibodies-fcm-testing-3.jpgAnti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1) Flow Cytometry analysis of A549 cells using anti-Golgin 97/GOLGA1 antibody (M13524-1). Overlay histogram showing A549 cells stained with M13524-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Golgin 97/GOLGA1 Antibody (M13524-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rnf20-picoband-trade-antibody-m03457-4-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03457-4-rnf20-primary-antibodies-wb-testing-1.jpgAnti-RNF20 Antibody Picoband® (monoclonal, 3C6E2) Western blot analysis of RNF20 using anti-RNF20 antibody (M03457-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RNF20 antigen affinity purified monoclonal antibody (Catalog # M03457-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RNF20 at approximately 114 kDa. The expected band size for RNF20 is at 114 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a1-anxa1-picoband-trade-antibody-m01451-3-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01451-3-anxa1-primary-antibodies-wb-testing-1.jpgAnti-Annexin A1/ANXA1 Antibody Picoband® (monoclonal, 6B7F8) Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U-87 MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Annexin A1/ANXA1 antigen affinity purified monoclonal antibody (Catalog # M01451-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 35-39 kDa. The expected band size for Annexin A1/ANXA1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01451-3-anxa1-primary-antibodies-ihc-testing-2.jpgAnti-Annexin A1/ANXA1 Antibody Picoband® (monoclonal, 6B7F8) IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3). Annexin A1/ANXA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A1/ANXA1 Antibody (M01451-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01451-3-anxa1-primary-antibodies-ihc-testing-3.jpgAnti-Annexin A1/ANXA1 Antibody Picoband® (monoclonal, 6B7F8) IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3). Annexin A1/ANXA1 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A1/ANXA1 Antibody (M01451-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01451-3-anxa1-primary-antibodies-ihc-testing-4.jpgAnti-Annexin A1/ANXA1 Antibody Picoband® (monoclonal, 6B7F8) IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (M01451-3). Annexin A1/ANXA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Annexin A1/ANXA1 Antibody (M01451-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01451-3-anxa1-primary-antibodies-fcm-testing-5.jpgAnti-Annexin A1/ANXA1 Antibody Picoband® (monoclonal, 6B7F8) Flow Cytometry analysis of A549 cells using anti-Annexin A1/ANXA1 antibody (M01451-3). Overlay histogram showing A549 cells stained with M01451-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Annexin A1/ANXA1 Antibody (M01451-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-desmoglein-2-dsg2-picoband-trade-antibody-m02035-2-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02035-2-dsg2-primary-antibodies-wb-testing-1.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® (monoclonal, 2B4D1) Western blot analysis of Desmoglein 2/DSG2 using anti-Desmoglein 2/DSG2 antibody (M02035-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Desmoglein 2/DSG2 antigen affinity purified monoclonal antibody (Catalog # M02035-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Desmoglein 2/DSG2 at approximately 160 kDa. The expected band size for Desmoglein 2/DSG2 is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02035-2-dsg2-primary-antibodies-ihc-testing-2.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® (monoclonal, 2B4D1) IHC analysis of Desmoglein 2/DSG2 using anti-Desmoglein 2/DSG2 antibody (M02035-2). Desmoglein 2/DSG2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmoglein 2/DSG2 Antibody (M02035-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02035-2-dsg2-primary-antibodies-ihc-testing-3.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® (monoclonal, 2B4D1) IHC analysis of Desmoglein 2/DSG2 using anti-Desmoglein 2/DSG2 antibody (M02035-2). Desmoglein 2/DSG2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmoglein 2/DSG2 Antibody (M02035-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02035-2-dsg2-primary-antibodies-ihc-testing-4.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® (monoclonal, 2B4D1) IHC analysis of Desmoglein 2/DSG2 using anti-Desmoglein 2/DSG2 antibody (M02035-2). Desmoglein 2/DSG2 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmoglein 2/DSG2 Antibody (M02035-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02035-2-dsg2-primary-antibodies-ihc-testing-5.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband® (monoclonal, 2B4D1) IHC analysis of Desmoglein 2/DSG2 using anti-Desmoglein 2/DSG2 antibody (M02035-2). Desmoglein 2/DSG2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Desmoglein 2/DSG2 Antibody (M02035-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp90-beta-hsp90ab1-picoband-trade-antibody-m01692-4-boster.html2026-03-24T05:36:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-wb-testing-1.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5) Western blot analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Hsp90 beta/HSP90AB1 antigen affinity purified monoclonal antibody (Catalog # M01692-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Hsp90 beta/HSP90AB1 at approximately 90 kDa. The expected band size for Hsp90 beta/HSP90AB1 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-ihc-testing-1.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5)IHC analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Hsp90 beta/HSP90AB1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-if-testing-2.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5) IF analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Hsp90 beta/HSP90AB1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-ihc-testing-2.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5)IHC analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Hsp90 beta/HSP90AB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-ihc-testing-3.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5)IHC analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Hsp90 beta/HSP90AB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-ihc-testing-4.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5)IHC analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Hsp90 beta/HSP90AB1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-ihc-testing-5.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5)IHC analysis of Hsp90 beta/HSP90AB1 using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Hsp90 beta/HSP90AB1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-4-hsp90ab1-primary-antibodies-fcm-testing-3.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband® (monoclonal, 7B7F5) Flow Cytometry analysis of CACO-2 cells using anti-Hsp90 beta/HSP90AB1 antibody (M01692-4). Overlay histogram showing CACO-2 cells stained with M01692-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Hsp90 beta/HSP90AB1 Antibody (M01692-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctbp2-picoband-trade-antibody-m02567-3-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-wb-testing-1.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) Western blot analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human COLO 320 whole cell lysates, Lane 4: human SW620 whole cell ysates, Lane 5: rat stomach tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CTBP2 antigen affinity purified monoclonal antibody (Catalog # M02567-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CTBP2 at approximately 49 kDa. The expected band size for CTBP2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-ihc-testing-2.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) IHC analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-ihc-testing-3.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) IHC analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-ihc-testing-4.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) IHC analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-ihc-testing-5.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) IHC analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-ihc-testing-6.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) IHC analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-ihc-testing-7.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) IHC analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-ihc-testing-8.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) IHC analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-if-testing-1.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1)IF analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-if-testing-2.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1)IF analysis of CTBP2 using anti-CTBP2 antibody (M02567-3). CTBP2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-CTBP2 Antibody (M02567-3) overnight at 4°Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02567-3-ctbp2-primary-antibodies-fcm-testing-9.jpgAnti-CTBP2 Antibody Picoband® (monoclonal, 7F3E1) Flow Cytometry analysis of U87 cells using anti-CTBP2 antibody (M02567-3). Overlay histogram showing U87 cells stained with M02567-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CTBP2 Antibody (M02567-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-transferrin-tf-picoband-trade-antibody-m00094-5-boster.html2026-03-17T05:14:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00094-5-tf-primary-antibodies-wb-testing-1.jpgAnti-Transferrin/TF Antibody Picoband® (monoclonal, 7I11B10) Western blot analysis of Transferrin/TF using anti-Transferrin/TF antibody (M00094-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Transferrin/TF antigen affinity purified monoclonal antibody (Catalog # M00094-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Transferrin/TF at approximately 77 kDa. The expected band size for Transferrin/TF is at 77 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-xrcc1-picoband-trade-antibody-m00571-2-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00571-2-xrcc1-primary-antibodies-wb-testing-1.jpgAnti-XRCC1 Antibody Picoband® (monoclonal, 10E10) Western blot analysis of XRCC1 using anti-XRCC1 antibody (M00571-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-XRCC1 antigen affinity purified monoclonal antibody (Catalog # M00571-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for XRCC1 at approximately 95 kDa. The expected band size for XRCC1 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00571-2-xrcc1-primary-antibodies-if-testing-2.jpgAnti-XRCC1 Antibody Picoband® (monoclonal, 10E10) IF analysis of XRCC1 using anti-XRCC1 antibody (M00571-2). XRCC1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-XRCC1 Antibody (M00571-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00571-2-xrcc1-primary-antibodies-fcm-testing-3.jpgAnti-XRCC1 Antibody Picoband® (monoclonal, 10E10) Flow Cytometry analysis of Hela cells using anti-XRCC1 antibody (M00571-2). Overlay histogram showing Hela cells stained with M00571-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-XRCC1 Antibody (M00571-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-lamin-a-c-lmna-picoband-trade-antibody-m00438-6-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-6-lmna-primary-antibodies-wb-testing-1.jpgAnti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) Western blot analysis of Lamin A+C/LMNA using anti-Lamin A+C/LMNA antibody (M00438-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Lamin A+C/LMNA antigen affinity purified monoclonal antibody (Catalog # M00438-6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Lamin A+C/LMNA at approximately 74 kDa. The expected band size for Lamin A+C/LMNA is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-6-lmna-primary-antibodies-ihc-testing-2.jpgAnti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) IHC analysis of Lamin A+C/LMNA using anti-Lamin A+C/LMNA antibody (M00438-6). Lamin A+C/LMNA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Lamin A+C/LMNA Antibody (M00438-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-6-lmna-primary-antibodies-ihc-testing-3.jpgAnti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) IHC analysis of Lamin A+C/LMNA using anti-Lamin A+C/LMNA antibody (M00438-6). Lamin A+C/LMNA was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Lamin A+C/LMNA Antibody (M00438-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-6-lmna-primary-antibodies-ihc-testing-4.jpgAnti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) IHC analysis of Lamin A+C/LMNA using anti-Lamin A+C/LMNA antibody (M00438-6). Lamin A+C/LMNA was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Lamin A+C/LMNA Antibody (M00438-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-6-lmna-primary-antibodies-ihc-testing-5.jpgAnti-Lamin A+C/LMNA Antibody Picoband® (monoclonal, 5F3C12) IHC analysis of Lamin A+C/LMNA using anti-Lamin A+C/LMNA antibody (M00438-6). Lamin A+C/LMNA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Lamin A+C/LMNA Antibody (M00438-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-sp1-picoband-trade-antibody-m00110-2-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-wb-testing-1.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) Western blot analysis of SP1 using anti-SP1 antibody (M00110-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SP1 antigen affinity purified monoclonal antibody (Catalog # M00110-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SP1 at approximately 90 kDa. The expected band size for SP1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-ihc-testing-2.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IHC analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-ihc-testing-3.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IHC analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-ihc-testing-4.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IHC analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-ihc-testing-5.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IHC analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-ihc-testing-6.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IHC analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-ihc-testing-7.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IHC analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-ihc-testing-8.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IHC analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-if-testing-9.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) IF analysis of SP1 using anti-SP1 antibody (M00110-2). SP1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-SP1 Antibody (M00110-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-2-sp1-primary-antibodies-fcm-testing-10.jpgAnti-SP1 Antibody Picoband® (monoclonal, 3C4C3) Flow Cytometry analysis of A431 cells using anti-SP1 antibody (M00110-2). Overlay histogram showing A431 cells stained with M00110-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SP1 Antibody (M00110-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-proteasome-20s-beta-7-psmb7-picoband-trade-antibody-m08095-2-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08095-2-psmb7-primary-antibodies-wb-testing-1.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® (monoclonal, 3H6C8) Western blot analysis of Proteasome 20S beta 7/PSMB7 using anti-Proteasome 20S beta 7/PSMB7 antibody (M08095-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Proteasome 20S beta 7/PSMB7 antigen affinity purified monoclonal antibody (Catalog # M08095-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Proteasome 20S beta 7/PSMB7 at approximately 25 kDa. The expected band size for Proteasome 20S beta 7/PSMB7 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08095-2-psmb7-primary-antibodies-fcm-testing-2.jpgAnti-Proteasome 20S beta 7/PSMB7 Antibody Picoband® (monoclonal, 3H6C8) Flow Cytometry analysis of CACO-2 cells using anti-PSMB7 antibody (M08095-2). Overlay histogram showing CACO-2 cells stained with M08095-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PSMB7 Antibody (M08095-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klhl12-picoband-trade-antibody-m08568-1-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08568-1-klhl12-primary-antibodies-wb-testing-1.jpgAnti-KLHL12 Antibody Picoband® (monoclonal, 2G11D1) Western blot analysis of KLHL12 using anti-KLHL12 antibody (M08568-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KLHL12 antigen affinity purified monoclonal antibody (Catalog # M08568-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KLHL12 at approximately 63 kDa. The expected band size for KLHL12 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08568-1-klhl12-primary-antibodies-ihc-testing-2.jpgAnti-KLHL12 Antibody Picoband® (monoclonal, 2G11D1) IHC analysis of KLHL12 using anti-KLHL12 antibody (M08568-1). KLHL12 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KLHL12 Antibody (M08568-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08568-1-klhl12-primary-antibodies-ihc-testing-3.jpgAnti-KLHL12 Antibody Picoband® (monoclonal, 2G11D1) IHC analysis of KLHL12 using anti-KLHL12 antibody (M08568-1). KLHL12 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-KLHL12 Antibody (M08568-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aspartate-aminotransferase-got1-picoband-trade-antibody-m04085-1-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04085-1-got1-primary-antibodies-wb-testing-1.jpgAnti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (monoclonal, 6B3B4) Western blot analysis of Aspartate Aminotransferase/GOT1 using anti-Aspartate Aminotransferase/GOT1 antibody (M04085-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Aspartate Aminotransferase/GOT1 antigen affinity purified monoclonal antibody (Catalog # M04085-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Aspartate Aminotransferase/GOT1 at approximately 43 kDa. The expected band size for Aspartate Aminotransferase/GOT1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04085-1-got1-primary-antibodies-ihc-testing-2.jpgAnti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (monoclonal, 6B3B4) IHC analysis of Aspartate Aminotransferase/GOT1 using anti-Aspartate Aminotransferase/GOT1 antibody (M04085-1). Aspartate Aminotransferase/GOT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aspartate Aminotransferase/GOT1 Antibody (M04085-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04085-1-got1-primary-antibodies-ihc-testing-3.jpgAnti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (monoclonal, 6B3B4) IHC analysis of Aspartate Aminotransferase/GOT1 using anti-Aspartate Aminotransferase/GOT1 antibody (M04085-1). Aspartate Aminotransferase/GOT1 was detected in a paraffin-embedded section of human renal pelvis squamous tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aspartate Aminotransferase/GOT1 Antibody (M04085-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04085-1-got1-primary-antibodies-ihc-testing-4.jpgAnti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (monoclonal, 6B3B4) IHC analysis of Aspartate Aminotransferase/GOT1 using anti-Aspartate Aminotransferase/GOT1 antibody (M04085-1). Aspartate Aminotransferase/GOT1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Aspartate Aminotransferase/GOT1 Antibody (M04085-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04085-1-got1-primary-antibodies-fcm-testing-5.jpgAnti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (monoclonal, 6B3B4) Flow Cytometry analysis of HepG2 cells using anti-Aspartate Aminotransferase/GOT1 antibody (M04085-1). Overlay histogram showing HepG2 cells stained with M04085-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Aspartate Aminotransferase/GOT1 Antibody (M04085-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rage-ager-picoband-trade-antibody-m03438-2-boster.html2026-04-06T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03438-2-ager-primary-antibodies-wb-testing-1.jpgAnti-RAGE/AGER Antibody Picoband® (monoclonal, 5C6C1) Western blot analysis of RAGE/AGER using anti-RAGE/AGER antibody (M03438-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RAGE/AGER antigen affinity purified monoclonal antibody (Catalog # M03438-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for RAGE/AGER at approximately 43 kDa. The expected band size for RAGE/AGER is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03438-2-ager-primary-antibodies-ihc-testing-2.jpgAnti-RAGE/AGER Antibody Picoband® (monoclonal, 5C6C1) IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (M03438-2). RAGE/AGER was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RAGE/AGER Antibody (M03438-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03438-2-ager-primary-antibodies-ihc-testing-3.jpgAnti-RAGE/AGER Antibody Picoband® (monoclonal, 5C6C1) IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (M03438-2). RAGE/AGER was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RAGE/AGER Antibody (M03438-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03438-2-ager-primary-antibodies-ihc-testing-4.jpgAnti-RAGE/AGER Antibody Picoband® (monoclonal, 5C6C1) IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (M03438-2). RAGE/AGER was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-RAGE/AGER Antibody (M03438-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03438-2-ager-primary-antibodies-fcm-testing-5.jpgAnti-RAGE/AGER Antibody Picoband® (monoclonal, 5C6C1) Flow Cytometry analysis of Jurkat cells using anti-RAGE/AGER antibody (M03438-2). Overlay histogram showing Jurkat cells stained with M03438-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RAGE/AGER Antibody (M03438-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klf2-mouse-monoclonal-antibody-clone-id-oti3d12-m01046-1-boster.html2026-03-16T05:08:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01046-1-anti-klf2-mouse-monoclonal-antibody-clone-id-oti3d12-wb-testing-1.jpgAnti-KLF2 Mouse Monoclonal Antibody [Clone ID: OTI3D12]HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF2. https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-rel1-antibody-dz41346-boster.html2026-03-10T04:35:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-abi2-picoband-trade-antibody-a04302-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04302-abi2-primary-antibodies-ihc-testing-4.jpgAnti-ABI2 Antibody Picoband®IHC analysis of ABI2 using anti-ABI2 antibody (A04302). ABI2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABI2 Antibody (A04302) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04302-abi2-primary-antibodies-wb-testing-1_1.jpgAnti-ABI2 Antibody Picoband® Western blot analysis of ABI2 using anti-ABI2 antibody (A04302). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI2 antigen affinity purified polyclonal antibody (Catalog # A04302) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABI2 at approximately 56-70 kDa. The expected band size for ABI2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04302-abi2-primary-antibodies-ihc-testing-2.jpgAnti-ABI2 Antibody Picoband® IHC analysis of ABI2 using anti-ABI2 antibody (A04302). ABI2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABI2 Antibody (A04302) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04302-abi2-primary-antibodies-ihc-testing-3.jpgAnti-ABI2 Antibody Picoband® IHC analysis of ABI2 using anti-ABI2 antibody (A04302). ABI2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABI2 Antibody (A04302) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04302-abi2-primary-antibodies-if-testing-4.jpgAnti-ABI2 Antibody Picoband® IF analysis of SNF2H/SMARCA5 and Tubulin beta using anti-SNF2H/SMARCA5 antibody (A04302) and anti-Tubulin beta antibody (M05613-4). SNF2H/SMARCA5 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNF2H/SMARCA5 Antibody (A04302) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1031) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04302-abi2-primary-antibodies-fcm-testing-5.jpgAnti-ABI2 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-ABI2 antibody (A04302). Overlay histogram showing SH-SY5Y cells stained with A04302 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABI2 Antibody (A04302, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-claudin-1-cldn1-picoband-trade-antibody-a01585-3-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01585-3-cldn1-primary-antibodies-wb-testing-1.jpgAnti-Claudin 1/CLDN1 Antibody Picoband® Western blot analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody (A01585-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 1/CLDN1 antigen affinity purified polyclonal antibody (Catalog # A01585-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 1/CLDN1 at approximately 22 kDa. The expected band size for Claudin 1/CLDN1 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-col6a2-picoband-trade-antibody-a03194-3-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-3-col6a2-primary-antibodies-wb-testing-1.jpgAnti-col6a2 Antibody Picoband® Western blot analysis of Col6a2 using anti-Col6a2 antibody (A03194-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish tissue lysates, Lane 2: zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Col6a2 antigen affinity purified polyclonal antibody (Catalog # A03194-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Col6a2 at approximately 140 kDa. The expected band size for Col6a2 is at 140 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03194-3-col6a2-primary-antibodies-ihc-testing-2.jpgAnti-col6a2 Antibody Picoband® IHC analysis of Col6a2 using anti-Col6a2 antibody (A03194-3). Col6a2 was detected in a paraffin-embedded section of zebrafish tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Col6a2 Antibody (A03194-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-eif2ak1-picoband-trade-antibody-a05465-2-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05465-2-eif2ak1-primary-antibodies-wb-testing-1.jpgAnti-EIF2AK1 Antibody Picoband® Western blot analysis of EIF2AK1 using anti-EIF2AK1 antibody (A05465-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2AK1 antigen affinity purified polyclonal antibody (Catalog # A05465-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2AK1 at approximately 71 kDa. The expected band size for EIF2AK1 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05465-2-eif2ak1-primary-antibodies-ihc-testing-2.jpgAnti-EIF2AK1 Antibody Picoband® IHC analysis of EIF2AK1 using anti-EIF2AK1 antibody (A05465-2). EIF2AK1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF2AK1 Antibody (A05465-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05465-2-eif2ak1-primary-antibodies-ihc-testing-3.jpgAnti-EIF2AK1 Antibody Picoband® IHC analysis of EIF2AK1 using anti-EIF2AK1 antibody (A05465-2). EIF2AK1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF2AK1 Antibody (A05465-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05465-2-eif2ak1-primary-antibodies-ihc-testing-4.jpgAnti-EIF2AK1 Antibody Picoband® IHC analysis of EIF2AK1 using anti-EIF2AK1 antibody (A05465-2). EIF2AK1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF2AK1 Antibody (A05465-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05465-2-eif2ak1-primary-antibodies-if-testing-5.jpgAnti-EIF2AK1 Antibody Picoband® IF analysis of EIF2AK1 using anti-EIF2AK1 antibody (A05465-2). EIF2AK1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EIF2AK1 Antibody (A05465-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cd105-eng-picoband-trade-antibody-a02997-3-boster.html2026-03-17T05:14:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02997-3-eng-primary-antibodies-wb-testing-1.jpgAnti-CD105/ENG Antibody Picoband® Western blot analysis of CD105/ENG using anti-CD105/ENG antibody (A02997-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD105/ENG antigen affinity purified polyclonal antibody (Catalog # A02997-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD105/ENG at approximately 95 kDa. The expected band size for CD105/ENG is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02997-3-eng-primary-antibodies-fcm-testing-7.pngAnti-CD105/ENG Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-CD105/ENG antibody (A02997-3). Overlay histogram showing U87 cells stained with A02997-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD105/ENG Antibody (A02997-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/c/a/casestudy_a02997-3.jpgAnti-CD105/ENG Antibody Picoband®https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02997-3-eng-primary-antibodies-ihc-testing-2_1.jpgAnti-CD105/ENG Antibody Picoband® IHC analysis of CD105/ENG using anti-CD105/ENG antibody (A02997-3). CD105/ENG was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD105/ENG Antibody (A02997-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02997-3-eng-primary-antibodies-ihc-testing-3_1.jpgAnti-CD105/ENG Antibody Picoband® IHC analysis of CD105/ENG using anti-CD105/ENG antibody (A02997-3). CD105/ENG was detected in a paraffin-embedded section of human lung cancer. tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD105/ENG Antibody (A02997-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02997-3-eng-primary-antibodies-ihc-testing-4_1.jpgAnti-CD105/ENG Antibody Picoband® IHC analysis of CD105/ENG using anti-CD105/ENG antibody (A02997-3). CD105/ENG was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD105/ENG Antibody (A02997-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02997-3-eng-primary-antibodies-ihc-testing-5_1.jpgAnti-CD105/ENG Antibody Picoband® IHC analysis of CD105/ENG using anti-CD105/ENG antibody (A02997-3). CD105/ENG was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD105/ENG Antibody (A02997-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02997-3-eng-primary-antibodies-if-testing-6.jpgAnti-CD105/ENG Antibody Picoband® IF analysis of CD105/ENG using anti-CD105/ENG antibody (A02997-3). CD105/ENG was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD105/ENG Antibody (A02997-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-fgfr2-picoband-trade-antibody-a00231-3-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00231-3-fgfr2-primary-antibodies-wb-testing-1.jpgAnti-FGFR2 Antibody Picoband® Western blot analysis of FGFR2 using anti-FGFR2 antibody (A00231-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR2 antigen affinity purified polyclonal antibody (Catalog # A00231-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR2 at approximately 135 kDa. The expected band size for FGFR2 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00231-3-fgfr2-primary-antibodies-fcm-testing-2.pngAnti-FGFR2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-FGFR2 antibody (A00231-3). Overlay histogram showing U20S cells stained with A00231-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FGFR2 Antibody (A00231-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-siae-picoband-trade-antibody-a08046-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08046-siae-primary-antibodies-wb-testing-1_1.jpgAnti-SIAE Antibody Picoband®Western blot analysis of SIAE using anti-SIAE antibody (A08046). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIAE antigen affinity purified polyclonal antibody (A08046) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIAE at approximately 70 kDa. The expected band size for SIAE is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08046-siae-primary-antibodies-ihc-testing-1.jpgAnti-SIAE Antibody Picoband®IHC analysis of SIAE using anti-SIAE antibody (A08046). SIAE was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIAE Antibody (A08046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08046-siae-primary-antibodies-ihc-testing-2_1.jpgAnti-SIAE Antibody Picoband®IHC analysis of SIAE using anti-SIAE antibody (A08046). SIAE was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIAE Antibody (A08046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08046-siae-primary-antibodies-if-testing-1.jpgAnti-SIAE Antibody Picoband®IF analysis of SIAE using anti-SIAE antibody (A08046). SIAE was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIAE Antibody (A08046) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08046-siae-primary-antibodies-fcm-testing-1.jpgAnti-SIAE Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SIAE antibody (A08046). Overlay histogram showing HepG2 cells stained with A08046 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIAE Antibody (A08046, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08046-siae-primary-antibodies-fcm-testing-2.jpgAnti-SIAE Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-SIAE antibody (A08046). Overlay histogram showing RT4 cells stained with A08046 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIAE Antibody (A08046, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-siglec12-picoband-trade-antibody-a10550-1-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10550-1-siglec12-primary-antibodies-wb-testing-1.jpgAnti-SIGLEC12 Antibody Picoband® Western blot analysis of SIGLEC12 using anti-SIGLEC12 antibody (A10550-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIGLEC12 antigen affinity purified polyclonal antibody (Catalog # A10550-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIGLEC12 at approximately 70 kDa. The expected band size for SIGLEC12 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10550-1-siglec12-primary-antibodies-fcm-testing-2.pngAnti-SIGLEC12 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SIGLEC12 antibody (A10550-1). Overlay histogram showing HEL cells stained with A10550-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SIGLEC12 Antibody (A10550-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cat2-slc7a2-picoband-trade-antibody-a06193-1-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06193-1-slc7a2-primary-antibodies-wb-testing-1.jpgAnti-CAT2/SLC7A2 Antibody Picoband® Western blot analysis of CAT2/SLC7A2 using anti-CAT2/SLC7A2 antibody (A06193-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat ovary tissue lysates, Lane 4: mouse skeletal muscle tissue lysates, Lane 5: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAT2/SLC7A2 antigen affinity purified polyclonal antibody (Catalog # A06193-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAT2/SLC7A2 at approximately 100 kDa. The expected band size for CAT2/SLC7A2 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06193-1-slc7a2-primary-antibodies-fcm-testing-2.pngAnti-CAT2/SLC7A2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CAT2/SLC7A2 antibody (A06193-1). Overlay histogram showing HepG2 cells stained with A06193-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CAT2/SLC7A2 Antibody (A06193-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nadc-1-slc13a2-picoband-trade-antibody-a07831-1-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07831-1-slc13a2-primary-antibodies-wb-testing-1.jpgAnti-NaDC-1/SLC13A2 Antibody Picoband® Western blot analysis of NaDC-1/SLC13A2 using anti-NaDC-1/SLC13A2 antibody (A07831-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NaDC-1/SLC13A2 antigen affinity purified polyclonal antibody (Catalog # A07831-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NaDC-1/SLC13A2 at approximately 70 kDa. The expected band size for NaDC-1/SLC13A2 is at 64 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc17a4-picoband-trade-antibody-a13469-1-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13469-1-slc17a4-primary-antibodies-wb-testing-1.jpgAnti-SLC17A4 Antibody Picoband® Western blot analysis of SLC17A4 using anti-SLC17A4 antibody (A13469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC17A4 antigen affinity purified polyclonal antibody (Catalog # A13469-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC17A4 at approximately 65 kDa. The expected band size for SLC17A4 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13469-1-slc17a4-primary-antibodies-fcm-testing-2.pngAnti-SLC17A4 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SLC17A4 antibody (A13469-1). Overlay histogram showing 293T cells stained with A13469-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC17A4 Antibody (A13469-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-vmat2-slc18a2-picoband-trade-antibody-a02232-1-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02232-1-slc18a2-primary-antibodies-wb-testing-1.jpgAnti-VMAT2/SLC18A2 Antibody Picoband® Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc20a1-picoband-trade-antibody-a03537-1-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-wb-testing-1.jpgAnti-SLC20A1 Antibody Picoband® Western blot analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC20A1 antigen affinity purified polyclonal antibody (Catalog # A03537-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC20A1 at approximately 85 kDa. The expected band size for SLC20A1 is at 74 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-fcimb-14-1322119-g007.jpgAnti-SLC20A1 Antibody Picoband®TLF-II protects fusiform vesicles and inhibits the occurrence of immune escape in bacteria. (A) Immunohistochemical staining of mouse bladders showing immunodetection of SLC20A1 (brown) in bladder epithelial cells (BECs; scale bar = 50 μm). (B) Western blot analysis of SLC20A1, Galectin-3, and Rab27b protein levels. (C) Quantitative analysis of the positive intensity of SLC20A1 in BECs. (D–F) Quantified protein bands of SLC20A1 (D) , Galectin-3 (E) , and Rab27b (F) with GAPDH as a control group. Data are represented as mean ± SEM of three independent experiments. Significance is indicated as the p value, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. NC group; *p<0.05, **p < 0.01, ***p <0.001 vs. UTI group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fcimb.2024.1322119/full'>38638825</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-fcimb-14-1322119-g008.jpgAnti-SLC20A1 Antibody Picoband®TLF-II reduces bacterial escape from fusiform vesicles into the cytoplasm. (A) Immunofluorescence staining of Rab27b, SLC20A1, UPEC-CFT073 and DAPI, along with co-localization of Rab27b, SLC20A1, and UPEC-CFT073, indicated by white arrows in the enlarged image. (B, C) Mean fluorescence intensity of Rab27b (B) and SLC20A1 (C) measured in randomly selected per view. Data are represented as mean ± SEM of three independent experiments. Significance is indicated as the p value, #p < 0.05, ##p < 0.01, ###p < 0.001 vs. NC group; **p < 0.01, ***p < 0.001 vs. UTI group. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fcimb.2024.1322119/full'>38638825</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-2.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-3.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-4.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-5.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-6.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-7.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-8.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-9.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-10.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-11.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-12.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-13.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-14.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-ihc-testing-15.jpgAnti-SLC20A1 Antibody Picoband® IHC analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03537-1-slc20a1-primary-antibodies-if-testing-16.jpgAnti-SLC20A1 Antibody Picoband® IF analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). SLC20A1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC20A1 Antibody (A03537-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-mitochondrial-dicarboxylate-carrier-slc25a10-picoband-trade-antibody-a10727-3-boster.html2026-04-01T05:01:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-wb-testing-1.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® Western blot analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antigen affinity purified polyclonal antibody (Catalog # A10727-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitochondrial Dicarboxylate Carrier/SLC25A10 at approximately 29-31 kDa. The expected band size for Mitochondrial Dicarboxylate Carrier/SLC25A10 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-2.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-3.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-4.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-5.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-6.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-7.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-8.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-9.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-10.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-11.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-12.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-ihc-testing-13.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IHC analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10727-3-slc25a10-primary-antibodies-if-testing-14.jpgAnti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® IF analysis of Mitochondrial Dicarboxylate Carrier/SLC25A10 using anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 antibody (A10727-3). Mitochondrial Dicarboxylate Carrier/SLC25A10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Mitochondrial Dicarboxylate Carrier/SLC25A10 Antibody (A10727-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-citrin-slc25a13-picoband-trade-antibody-a03476-2-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-wb-testing-1.jpgAnti-Citrin/SLC25A13 Antibody Picoband® Western blot analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Citrin/SLC25A13 antigen affinity purified polyclonal antibody (Catalog # A03476-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Citrin/SLC25A13 at approximately 70 kDa. The expected band size for Citrin/SLC25A13 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-14.jpgAnti-Citrin/SLC25A13 Antibody Picoband®IHC analysis of SLC25A13 using anti-SLC25A13 antibody (A03476-2). SLC25A13 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-2.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-3.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-4.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-5.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-6.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-7.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-8.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-9.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-10.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-11.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-12.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-ihc-testing-13.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IHC analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-if-testing-14.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IF analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-if-testing-15.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IF analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-fcm-testing-17.pngAnti-Citrin/SLC25A13 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-Citrin/SLC25A13 antibody (A03476-2). Overlay histogram showing JK cells stained with A03476-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Citrin/SLC25A13 Antibody (A03476-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-if-testing-16.jpgAnti-Citrin/SLC25A13 Antibody Picoband® IF analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Citrin/SLC25A13 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Citrin/SLC25A13 Antibody (A03476-2) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03476-2-slc25a13-primary-antibodies-fcm-testing-18.pngAnti-Citrin/SLC25A13 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Citrin/SLC25A13 antibody (A03476-2). Overlay histogram showing U87 cells stained with A03476-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Citrin/SLC25A13 Antibody (A03476-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc25a23-picoband-trade-antibody-a08997-1-boster.html2026-03-17T05:14:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08997-1-slc25a3-primary-antibodies-wb-testing-1.jpgAnti-SLC25A23 Antibody Picoband® Western blot analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tisue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A23 antigen affinity purified polyclonal antibody (Catalog # A08997-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A23 at approximately 48-54 kDa. The expected band size for SLC25A23 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08997-1-slc25a23-primary-antibodies-ihc-testing-4.jpgAnti-SLC25A23 Antibody Picoband®IHC analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1). SLC25A23 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A23 Antibody (A08997-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08997-1-slc25a23-primary-antibodies-ihc-testing-5.jpgAnti-SLC25A23 Antibody Picoband®IHC analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1). SLC25A23 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A23 Antibody (A08997-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08997-1-slc25a3-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A23 Antibody Picoband® IHC analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1). SLC25A23 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A23 Antibody (A08997-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08997-1-slc25a3-primary-antibodies-ihc-testing-3.jpgAnti-SLC25A23 Antibody Picoband® IHC analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1). SLC25A23 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A23 Antibody (A08997-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-slc25a24-picoband-trade-antibody-a08355-1-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-wb-testing-1.jpgAnti-SLC25A24 Antibody Picoband® Western blot analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A24 antigen affinity purified polyclonal antibody (Catalog # A08355-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A24 at approximately 45 kDa. The expected band size for SLC25A24 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-3.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-4.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-5.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-6.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-7.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-8.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-9.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-10.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-11.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-ihc-testing-12.jpgAnti-SLC25A24 Antibody Picoband® IHC analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-if-testing-13.jpgAnti-SLC25A24 Antibody Picoband® IF analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). SLC25A24 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC25A24 Antibody (A08355-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08355-1-slc25a24-primary-antibodies-fcm-testing-14.pngAnti-SLC25A24 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-SLC25A24 antibody (A08355-1). Overlay histogram showing MCF-7 cells stained with A08355-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC25A24 Antibody (A08355-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc25a29-picoband-trade-antibody-a11796-1-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11796-1-slc25a29-primary-antibodies-wb-testing-1.jpgAnti-SLC25A29 Antibody Picoband® Western blot analysis of SLC25A29 using anti-SLC25A29 antibody (A11796-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A29 antigen affinity purified polyclonal antibody (Catalog # A11796-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A29 at approximately 35 kDa. The expected band size for SLC25A29 is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-fatp3-slc27a3-picoband-trade-antibody-a12345-1-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12345-1-slc27a3-primary-antibodies-wb-testing-1.jpgAnti-FATP3/SLC27A3 Antibody Picoband® Western blot analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP3/SLC27A3 antigen affinity purified polyclonal antibody (Catalog # A12345-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP3/SLC27A3 at approximately 70-79 kDa. The expected band size for FATP3/SLC27A3 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12345-1-slc27a3-primary-antibodies-ihc-testing-2.jpgAnti-FATP3/SLC27A3 Antibody Picoband® IHC analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1). FATP3/SLC27A3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP3/SLC27A3 Antibody (A12345-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12345-1-slc27a3-primary-antibodies-ihc-testing-3.jpgAnti-FATP3/SLC27A3 Antibody Picoband® IHC analysis of FATP3/SLC27A3 using anti-FATP3/SLC27A3 antibody (A12345-1). FATP3/SLC27A3 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP3/SLC27A3 Antibody (A12345-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-fatp4-slc27a4-picoband-trade-antibody-a05299-2-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05299-2-slc27a4-primary-antibodies-wb-testing-1.jpgAnti-FATP4/SLC27A4 Antibody Picoband® Western blot analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP4/SLC27A4 antigen affinity purified polyclonal antibody (Catalog # A05299-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP4/SLC27A4 at approximately 70 kDa. The expected band size for FATP4/SLC27A4 is at 72 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05299-2-slc27a4-primary-antibodies-ihc-testing-5.jpgAnti-FATP4/SLC27A4 Antibody Picoband®IHC analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2). FATP4/SLC27A4 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP4/SLC27A4 Antibody (A05299-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05299-2-slc27a4-primary-antibodies-ihc-testing-2.jpgAnti-FATP4/SLC27A4 Antibody Picoband® IHC analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2). FATP4/SLC27A4 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP4/SLC27A4 Antibody (A05299-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05299-2-slc27a4-primary-antibodies-ihc-testing-3.jpgAnti-FATP4/SLC27A4 Antibody Picoband® IHC analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2). FATP4/SLC27A4 was detected in a paraffin-embedded section of human prostatic carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP4/SLC27A4 Antibody (A05299-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05299-2-slc27a4-primary-antibodies-ihc-testing-4.jpgAnti-FATP4/SLC27A4 Antibody Picoband® IHC analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2). FATP4/SLC27A4 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATP4/SLC27A4 Antibody (A05299-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-slc30a2-picoband-trade-antibody-a06643-2-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06643-2-slc30a2-primary-antibodies-wb-testing-1.jpgAnti-SLC30A2 Antibody Picoband® Western blot analysis of SLC30A2 using anti-SLC30A2 antibody (A06643-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse eye tisue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC30A2 antigen affinity purified polyclonal antibody (Catalog # A06643-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC30A2 at approximately 36 kDa. The expected band size for SLC30A2 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc35a4-picoband-trade-antibody-a17258-1-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17258-1-slc35a4-primary-antibodies-wb-testing-1.jpgAnti-SLC35A4 Antibody Picoband® Western blot analysis of SLC35A4 using anti-SLC35A4 antibody (A17258-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC35A4 antigen affinity purified polyclonal antibody (Catalog # A17258-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC35A4 at approximately 36 kDa. The expected band size for SLC35A4 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slc35d1-picoband-trade-antibody-a11935-2-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11935-2-slc35d1-primary-antibodies-wb-testing-1.jpgAnti-SLC35D1 Antibody Picoband® Western blot analysis of SLC35D1 using anti-SLC35D1 antibody (A11935-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC35D1 antigen affinity purified polyclonal antibody (Catalog # A11935-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC35D1 at approximately 39 kDa. The expected band size for SLC35D1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11935-2-slc35d1-primary-antibodies-fcm-testing-2.pngAnti-SLC35D1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SLC35D1 antibody (A11935-2). Overlay histogram showing U87 cells stained with A11935-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC35D1 Antibody (A11935-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slc35e4-picoband-trade-antibody-a18522-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18522-slc35e4-primary-antibodies-wb-testing-1.jpgAnti-SLC35E4 Antibody Picoband® Western blot analysis of SLC35E4 using anti-SLC35E4 antibody (A18522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC35E4 antigen affinity purified polyclonal antibody (Catalog # A18522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC35E4 at approximately 40 kDa. The expected band size for SLC35E4 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18522-slc35e4-primary-antibodies-fcm-testing-2.pngAnti-SLC35E4 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SLC35E4 antibody (A18522). Overlay histogram showing PC-3 cells stained with A18522 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC35E4 Antibody (A18522, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-zip7-slc39a7-picoband-trade-antibody-a07719-4-boster.html2026-03-17T05:14:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07719-4-slc39a7-primary-antibodies-wb-testing-1.jpgAnti-ZIP7/SLC39A7 Antibody Picoband® Western blot analysis of ZIP7/SLC39A7 using anti-ZIP7/SLC39A7 antibody (A07719-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIP7/SLC39A7 antigen affinity purified polyclonal antibody (Catalog # A07719-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIP7/SLC39A7 at approximately 50 kDa. The expected band size for ZIP7/SLC39A7 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07719-4-slc39a7-primary-antibodies-if-testing-2.jpgAnti-ZIP7/SLC39A7 Antibody Picoband® IF analysis of ZIP7/SLC39A7 using anti-ZIP7/SLC39A7 antibody (A07719-4). ZIP7/SLC39A7 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ZIP7/SLC39A7 Antibody (A07719-4) overnight at 4°C.Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-mate-1-slc47a1-picoband-trade-antibody-a04529-2-boster.html2026-03-17T05:14:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04529-2-slc47a1-primary-antibodies-wb-testing-1.jpgAnti-MATE-1/SLC47A1 Antibody Picoband® Western blot analysis of MATE-1/SLC47A1 using anti-MATE-1/SLC47A1 antibody (A04529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATE-1/SLC47A1 antigen affinity purified polyclonal antibody (Catalog # A04529-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATE-1/SLC47A1 at approximately 32 kDa. The expected band size for MATE-1/SLC47A1 is at 62 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-slmap-picoband-trade-antibody-a07262-boster.html2026-03-17T05:14:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07262-slmap-primary-antibodies-wb-testing-1.jpgAnti-SLMAP Antibody Picoband® Western blot analysis of SLMAP using anti-SLMAP antibody (A07262). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLMAP antigen affinity purified polyclonal antibody (Catalog # A07262) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLMAP at approximately 95 kDa. The expected band size for SLMAP is at 95 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07262-slmap-primary-antibodies-fcm-testing-2.pngAnti-SLMAP Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SLMAP antibody (A07262). Overlay histogram showing K562 cells stained with A07262 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLMAP Antibody (A07262, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-smap1-picoband-trade-antibody-a11009-boster.html2026-03-17T05:14:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11009-smap1-primary-antibodies-wb-testing-1.jpgAnti-SMAP1 Antibody Picoband® Western blot analysis of SMAP1 using anti-SMAP1 antibody (A11009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAP1 antigen affinity purified polyclonal antibody (Catalog # A11009) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAP1 at approximately 60 kDa. The expected band size for SMAP1 is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-smarca2-brm-picoband-trade-antibody-a01888-3-boster.html2026-03-17T05:14:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-wb-testing-1.jpgAnti-SMARCA2/BRM Antibody Picoband® Western blot analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCA2/BRM antigen affinity purified polyclonal antibody (Catalog # A01888-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCA2/BRM at approximately 210 kDa. The expected band size for SMARCA2/BRM is at 181 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-2.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-3.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-4.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-5.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-6.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-7.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human glioblastom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-8.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-9.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-10.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-11.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-12.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-ihc-testing-13.jpgAnti-SMARCA2/BRM Antibody Picoband® IHC analysis of SMARCA2/BRM using anti-SMARCA2/BRM antibody (A01888-3). SMARCA2/BRM was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA2/BRM Antibody (A01888-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-if-testing-14.jpgAnti-SMARCA2/BRM Antibody Picoband® IF analysis of SMARCA2/BRM and Tubulin beta using anti-SMARCA2/BRM antibody (A01888-3) and anti-Tubulin beta antibody (M05613-4). SMARCA2/BRM and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMARCA2/BRM Antibody (A01888-3) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01888-3-smarca2-primary-antibodies-fcm-testing-15.pngAnti-SMARCA2/BRM Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-SMARCA2/BRM antibody (A01888-3). Overlay histogram showing THP-1 cells stained with A01888-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCA2/BRM Antibody (A01888-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snf2h-smarca5-picoband-trade-antibody-a02687-1-boster.html2026-03-17T05:14:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-wb-testing-1.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® Western blot analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF2H/SMARCA5 antigen affinity purified polyclonal antibody (Catalog # A02687-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF2H/SMARCA5 at approximately 122 kDa. The expected band size for SNF2H/SMARCA5 is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-2.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-3.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-4.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-5.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-6.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-7.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-8.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-9.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-10.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-ihc-testing-11.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IHC analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). SNF2H/SMARCA5 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-if-testing-12.jpgAnti-SNF2H/SMARCA5 Antibody Picoband® IF analysis of SNF2H/SMARCA5 and Tubulin beta using anti-SNF2H/SMARCA5 antibody (A02687-1) and anti-Tubulin beta antibody (M05613-4). SNF2H/SMARCA5 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02687-1-smarca5-primary-antibodies-fcm-testing-13.pngAnti-SNF2H/SMARCA5 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-SNF2H/SMARCA5 antibody (A02687-1). Overlay histogram showing THP-1 cells stained with A02687-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNF2H/SMARCA5 Antibody (A02687-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-smc1a-picoband-trade-antibody-a02148-5-boster.html2026-03-17T05:14:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02148-5-smc1a-primary-antibodies-wb-testing-1.jpgAnti-SMC1A Antibody Picoband® Western blot analysis of SMC1A using anti-SMC1A antibody (A02148-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A antigen affinity purified polyclonal antibody (Catalog # A02148-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1A at approximately 150 kDa. The expected band size for SMC1A is at 143 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02148-5-smc1a-primary-antibodies-if-testing-2.jpgAnti-SMC1A Antibody Picoband® IF analysis of SMC1A and Tubulin beta using anti-SMC1A antibody (A02148-5) and anti-Tubulin beta antibody (M05613-4). SMC1A and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMC1A Antibody (A02148-5) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-smc2-picoband-trade-antibody-a04804-2-boster.html2026-03-17T05:14:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04804-2-smc2-primary-antibodies-wb-testing-1.jpgAnti-SMC2 Antibody Picoband® Western blot analysis of SMC2 using anti-SMC2 antibody (A04804-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC2 antigen affinity purified polyclonal antibody (Catalog # A04804-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC2 at approximately 136 kDa. The expected band size for SMC2 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04804-2-smc2-primary-antibodies-if-testing-2.jpgAnti-SMC2 Antibody Picoband® IF analysis of SMC2 and Tubulin beta using anti-SMC2 antibody (A04804-2) and anti-Tubulin beta antibody (M05613-4). SMC2 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMC2 Antibody (A04804-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04804-2-smc2-primary-antibodies-fcm-testing-3.pngAnti-SMC2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SMC2 antibody (A04804-2). Overlay histogram showing 293T cells stained with A04804-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC2 Antibody (A04804-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-smc5-picoband-trade-antibody-a01285-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01285-smc5-primary-antibodies-wb-testing-1.jpgAnti-SMC5 Antibody Picoband® Western blot analysis of SMC5 using anti-SMC5 antibody (A01285). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC5 antigen affinity purified polyclonal antibody (Catalog # A01285) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC5 at approximately 130 kDa. The expected band size for SMC5 is at 129 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01285-smc5-primary-antibodies-if-testing-2.jpgAnti-SMC5 Antibody Picoband® IF analysis of SMC5 using anti-SMC5 antibody (A01285) and anti-Beta Tubulin antibody (M01857-3). SMC5 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMC5 Antibody (A01285) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01285-smc5-primary-antibodies-fcm-testing-3.pngAnti-SMC5 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-SMC5 antibody (A01285). Overlay histogram showing THP-1 cells stained with A01285 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC5 Antibody (A01285, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-smc6-picoband-trade-antibody-a01554-2-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-2-smc6-primary-antibodies-wb-testing-1.jpgAnti-SMC6 Antibody Picoband® Western blot analysis of SMC6 using anti-SMC6 antibody (A01554-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC6 antigen affinity purified polyclonal antibody (Catalog # A01554-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC6 at approximately 130 kDa. The expected band size for SMC6 is at 126 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-2-smc6-primary-antibodies-ihc-testing-2.jpgAnti-SMC6 Antibody Picoband® IHC analysis of SMC6 using anti-SMC6 antibody (A01554-2). SMC6 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMC6 Antibody (A01554-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-2-smc6-primary-antibodies-if-testing-3.jpgAnti-SMC6 Antibody Picoband® IF analysis of SMC6 and Tubulin beta using anti-SMC6 antibody (A01554-2) and anti-Tubulin beta antibody (M05613-4). SMC6 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMC6 Antibody (A01554-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-2-smc6-primary-antibodies-fcm-testing-5.pngAnti-SMC6 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SMC6 antibody (A01554-2). Overlay histogram showing 293T cells stained with A01554-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC6 Antibody (A01554-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01554-2-smc6-primary-antibodies-if-testing-4.jpgAnti-SMC6 Antibody Picoband® IF analysis of SMC6 using anti-SMC6 antibody (A01554-2). SMC6 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SMC6 Antibody (A01554-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-smchd1-picoband-trade-antibody-a04751-1-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04751-1-smchd1-primary-antibodies-wb-testing-1.jpgAnti-SMCHD1 Antibody Picoband® Western blot analysis of SMCHD1 using anti-SMCHD1 antibody (A04751-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMCHD1 antigen affinity purified polyclonal antibody (Catalog # A04751-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMCHD1 at approximately 226 kDa. The expected band size for SMCHD1 is at 226 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04751-1-smchd1-primary-antibodies-if-testing-2.jpgAnti-SMCHD1 Antibody Picoband® IF analysis of SMCHD1 and Tubulin beta using anti-SMCHD1 antibody (A04514-2) and anti-Tubulin beta antibody (M05613-4). SMCHD1 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMCHD1 Antibody (A04514-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-smox-picoband-trade-antibody-a05519-2-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-wb-testing-1.jpgAnti-SMOX Antibody Picoband® Western blot analysis of SMOX using anti-SMOX antibody (A05519-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMOX at approximately 69 kDa. The expected band size for SMOX is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-2.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-1.jpgAnti-SMOX Antibody Picoband®IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-3.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-4.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-5.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-6.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-7.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-8.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-9.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-10.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-ihc-testing-11.jpgAnti-SMOX Antibody Picoband® IHC analysis of SMOX using anti-SMOX antibody (A05519-2). SMOX was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMOX Antibody (A05519-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-if-testing-14.jpgAnti-SMOX Antibody Picoband® IF analysis of SMOX using anti-SMOX antibody (A05519-2) and anti-Beta Tubulin antibody (M01857-3). SMOX was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMOX Antibody (A05519-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-fcm-testing-12.pngAnti-SMOX Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SMOX antibody (A05519-2). Overlay histogram showing HL-60 cells stained with A05519-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOX Antibody (A05519-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05519-2-smox-primary-antibodies-fcm-testing-13.pngAnti-SMOX Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-SMOX antibody (A05519-2). Overlay histogram showing MCF-7 cells stained with A05519-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOX Antibody (A05519-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spermine-synthase-sms-picoband-trade-antibody-a01831-1-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01831-1-sms-primary-antibodies-wb-testing-1.jpgAnti-Spermine synthase/SMS Antibody Picoband® Western blot analysis of Spermine Synthase/SMS using anti-Spermine Synthase/SMS antibody (A01831-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spermine Synthase/SMS antigen affinity purified polyclonal antibody (Catalog # A01831-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spermine Synthase/SMS at approximately 41 kDa. The expected band size for Spermine Synthase/SMS is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01831-1-sms-primary-antibodies-fcm-testing-2.pngAnti-Spermine synthase/SMS Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-SMS antibody (A01831-1). Overlay histogram showing A431 cells stained with A01831-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMS Antibody (A01831-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01831-1-sms-primary-antibodies-fcm-testing-3.pngAnti-Spermine synthase/SMS Antibody Picoband® Flow Cytometry analysis of JK cells using anti-SMS antibody (A01831-1). Overlay histogram showing JK cells stained with A01831-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMS Antibody (A01831-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snph-picoband-trade-antibody-a08882-1-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-wb-testing-1.jpgAnti-SNPH Antibody Picoband® Western blot analysis of SNPH using anti-SNPH antibody (A08882-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNPH antigen affinity purified polyclonal antibody (Catalog # A08882-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNPH at approximately 70 kDa. The expected band size for SNPH is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-10.jpgAnti-SNPH Antibody Picoband®IHC analysis of Syntaphilin/SNPH using anti-Syntaphilin/SNPH antibody (A08882-1). Syntaphilin/SNPH was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaphilin/SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-2.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-3.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-4.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-5.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-6.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-7.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-8.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08882-1-snph-primary-antibodies-ihc-testing-9.jpgAnti-SNPH Antibody Picoband® IHC analysis of SNPH using anti-SNPH antibody (A08882-1). SNPH was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNPH Antibody (A08882-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-snrnp27-picoband-trade-antibody-a14283-1-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-wb-testing-1.jpgAnti-SNRNP27 Antibody Picoband® Western blot analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP27 antigen affinity purified polyclonal antibody (Catalog # A14283-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP27 at approximately 23 kDa. The expected band size for SNRNP27 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-2.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-3.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-4.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-5.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-6.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-7.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-8.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-9.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-10.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-ihc-testing-11.jpgAnti-SNRNP27 Antibody Picoband® IHC analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-if-testing-12.jpgAnti-SNRNP27 Antibody Picoband® IF analysis of SNRNP27 and Tubulin beta using anti-SNRNP27 antibody (A14283-1) and anti-Tubulin beta antibody (M05613-4). SNRNP27 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRNP27 Antibody (A14283-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-if-testing-13.jpgAnti-SNRNP27 Antibody Picoband® IF analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-if-testing-14.jpgAnti-SNRNP27 Antibody Picoband® IF analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14283-1-snrnp27-primary-antibodies-if-testing-15.jpgAnti-SNRNP27 Antibody Picoband® IF analysis of SNRNP27 using anti-SNRNP27 antibody (A14283-1). SNRNP27 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRNP27 Antibody (A14283-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-snrnp40-picoband-trade-antibody-a12991-2-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-wb-testing-1.jpgAnti-SNRNP40 Antibody Picoband® Western blot analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP40 antigen affinity purified polyclonal antibody (Catalog # A12991-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP40 at approximately 37 kDa. The expected band size for SNRNP40 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-2.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-3.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-4.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-5.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-6.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-7.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-8.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-9.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-10.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-11.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-12.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-13.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-14.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-15.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-16.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-ihc-testing-17.jpgAnti-SNRNP40 Antibody Picoband® IHC analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-if-testing-18.jpgAnti-SNRNP40 Antibody Picoband® IF analysis of SNRNP40 and Tubulin beta using anti-SNRNP40 antibody (A12991-2) and anti-Tubulin beta antibody (M05613-4). SNRNP40 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRNP40 Antibody (A12991-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-if-testing-19.jpgAnti-SNRNP40 Antibody Picoband® IF analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). SNRNP40 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRNP40 Antibody (A12991-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-fcm-testing-20.pngAnti-SNRNP40 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SNRNP40 antibody (A12991-2). Overlay histogram showing 293T cells stained with A12991-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP40 Antibody (A12991-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12991-2-snrnp40-primary-antibodies-fcm-testing-21.pngAnti-SNRNP40 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-SNRNP40 antibody (A12991-2). Overlay histogram showing Daudi cells stained with A12991-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP40 Antibody (A12991-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snrnp200-picoband-trade-antibody-a04514-2-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-wb-testing-1.jpgAnti-SNRNP200 Antibody Picoband® Western blot analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP200 antigen affinity purified polyclonal antibody (Catalog # A04514-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP200 at approximately 245 kDa. The expected band size for SNRNP200 is at 245 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-2.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-3.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-4.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-5.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-6.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-7.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-8.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-9.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-10.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-11.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-12.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-13.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-14.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-15.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-16.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-17.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-ihc-testing-18.jpgAnti-SNRNP200 Antibody Picoband® IHC analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-if-testing-19.jpgAnti-SNRNP200 Antibody Picoband® IF analysis of SNRNP200 and Tubulin beta using anti-SNRNP200 antibody (A04514-2) and anti-Tubulin beta antibody (M05613-4). SNRNP200 and Tubulin beta was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRNP200 Antibody (A04514-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-if-testing-20.jpgAnti-SNRNP200 Antibody Picoband® IF analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). SNRNP200 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRNP200 Antibody (A04514-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-fcm-testing-21.pngAnti-SNRNP200 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-SNRNP200 antibody (A04514-2). Overlay histogram showing A431 cells stained with A04514-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP200 Antibody (A04514-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04514-2-snrnp200-primary-antibodies-fcm-testing-22.pngAnti-SNRNP200 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-SNRNP200 antibody (A04514-2). Overlay histogram showing ANA-1 cells stained with A04514-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRNP200 Antibody (A04514-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-u1a-snrpa-picoband-trade-antibody-a08780-2-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-wb-testing-1.jpgAnti-U1A/SNRPA Antibody Picoband® Western blot analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U1A/SNRPA antigen affinity purified polyclonal antibody (Catalog # A08780-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U1A/SNRPA at approximately 31 kDa. The expected band size for U1A/SNRPA is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-2.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-3.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-4.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-5.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-6.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-7.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-8.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-9.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-10.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-11.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-12.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-13.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-14.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-15.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-16.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-17.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-18.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-ihc-testing-19.jpgAnti-U1A/SNRPA Antibody Picoband® IHC analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-if-testing-20.jpgAnti-U1A/SNRPA Antibody Picoband® IF analysis of U1A/SNRPA and Tubulin beta using anti-U1A/SNRPA antibody (A08780-2) and anti-Tubulin beta antibody (M05613-4). U1A/SNRPA and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-U1A/SNRPA Antibody (A08780-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08780-2-snrpa-primary-antibodies-if-testing-21.jpgAnti-U1A/SNRPA Antibody Picoband® IF analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). U1A/SNRPA was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-U1A/SNRPA Antibody (A08780-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-snrpa1-picoband-trade-antibody-a10655-1-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-wb-testing-1.jpgAnti-SNRPA1 Antibody Picoband® Western blot analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPA1 antigen affinity purified polyclonal antibody (Catalog # A10655-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPA1 at approximately 28 kDa. The expected band size for SNRPA1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-2.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-3.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-4.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-5.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-6.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-7.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-8.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-9.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-10.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-11.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-ihc-testing-12.jpgAnti-SNRPA1 Antibody Picoband® IHC analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-if-testing-13.jpgAnti-SNRPA1 Antibody Picoband® IF analysis of SNRPA1 and Tubulin beta using anti-SNRPA1 antibody (A10655-1) and anti-Tubulin beta antibody (M05613-4). SNRPA1 and Tubulin beta was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRPA1 Antibody (A10655-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-if-testing-14.jpgAnti-SNRPA1 Antibody Picoband® IF analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-if-testing-15.jpgAnti-SNRPA1 Antibody Picoband® IF analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-if-testing-16.jpgAnti-SNRPA1 Antibody Picoband® IF analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-if-testing-17.jpgAnti-SNRPA1 Antibody Picoband® IF analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). SNRPA1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPA1 Antibody (A10655-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10655-1-snrpa1-primary-antibodies-fcm-testing-18.pngAnti-SNRPA1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-SNRPA1 antibody (A10655-1). Overlay histogram showing MCF-7 cells stained with A10655-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRPA1 Antibody (A10655-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-u1-c-snrpc-picoband-trade-antibody-a07728-boster.html2026-03-17T05:14:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-wb-testing-1.jpgAnti-U1-C/SNRPC Antibody Picoband® Western blot analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U1-C/SNRPC antigen affinity purified polyclonal antibody (Catalog # A07728) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U1-C/SNRPC at approximately 17 kDa. The expected band size for U1-C/SNRPC is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-2.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-3.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-4.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-5.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-6.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-7.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-8.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-9.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-10.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-11.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-ihc-testing-12.jpgAnti-U1-C/SNRPC Antibody Picoband® IHC analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-if-testing-13.jpgAnti-U1-C/SNRPC Antibody Picoband® IF analysis of U1-C/SNRPC and Tubulin beta using anti-U1-C/SNRPC antibody (A07728) and anti-Tubulin beta antibody (M05613-4). U1-C/SNRPC and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-U1-C/SNRPC Antibody (A07728) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-if-testing-14.jpgAnti-U1-C/SNRPC Antibody Picoband® IF analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). U1-C/SNRPC was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-U1-C/SNRPC Antibody (A07728) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07728-snrpc-primary-antibodies-fcm-testing-15.pngAnti-U1-C/SNRPC Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-U1-C/SNRPC antibody (A07728). Overlay histogram showing THP-1 cells stained with A07728 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-U1-C/SNRPC Antibody (A07728, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snrpd2-picoband-trade-antibody-a12026-1-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-wb-testing-1.jpgAnti-SNRPD2 Antibody Picoband® Western blot analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPD2 antigen affinity purified polyclonal antibody (Catalog # A12026-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPD2 at approximately 16 kDa. The expected band size for SNRPD2 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-2.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-3.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-4.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-5.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-6.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-7.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-8.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-9.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-10.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-11.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-ihc-testing-12.jpgAnti-SNRPD2 Antibody Picoband® IHC analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-if-testing-13.jpgAnti-SNRPD2 Antibody Picoband® IF analysis of SNRPD2 and Tubulin beta using anti-SNRPD2 antibody (A12026-1) and anti-Tubulin beta antibody (M05613-4). SNRPD2 and Tubulin beta was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRPD2 Antibody (A12026-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-if-testing-14.jpgAnti-SNRPD2 Antibody Picoband® IF analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-if-testing-15.jpgAnti-SNRPD2 Antibody Picoband® IF analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). SNRPD2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPD2 Antibody (A12026-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12026-1-snrpd2-primary-antibodies-fcm-testing-16.pngAnti-SNRPD2 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-SNRPD2 antibody (A12026-1). Overlay histogram showing JK cells stained with A12026-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNRPD2 Antibody (A12026-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sm-d3-snrpd3-picoband-trade-antibody-a09533-1-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-wb-testing-1.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® Western blot analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sm-D3/SNRPD3 antigen affinity purified polyclonal antibody (Catalog # A09533-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sm-D3/SNRPD3 at approximately 16 kDa. The expected band size for Sm-D3/SNRPD3 is at 14 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-ihc-testing-2.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Sm-D3/SNRPD3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sm-D3/SNRPD3 Antibody (A09533-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-ihc-testing-3.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Sm-D3/SNRPD3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sm-D3/SNRPD3 Antibody (A09533-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-ihc-testing-4.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sm-D3/SNRPD3 Antibody (A09533-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-ihc-testing-5.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sm-D3/SNRPD3 Antibody (A09533-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-ihc-testing-6.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sm-D3/SNRPD3 Antibody (A09533-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-ihc-testing-7.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® IHC analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sm-D3/SNRPD3 Antibody (A09533-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09533-1-snrpd3-primary-antibodies-if-testing-8.jpgAnti-Sm-D3/SNRPD3 Antibody Picoband® IF analysis of Sm-D3/SNRPD3 using anti-Sm-D3/SNRPD3 antibody (A09533-1). Sm-D3/SNRPD3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Sm-D3/SNRPD3 Antibody (A09533-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-snurportin1-snupn-picoband-trade-antibody-a07714-1-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07714-1-snupn-primary-antibodies-wb-testing-1.jpgAnti-SNURPORTIN1/SNUPN Antibody Picoband® Western blot analysis of SNURPORTIN1/SNUPN using anti-SNURPORTIN1/SNUPN antibody (A07714-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNURPORTIN1/SNUPN antigen affinity purified polyclonal antibody (Catalog # A07714-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNURPORTIN1/SNUPN at approximately 41 kDa. The expected band size for SNURPORTIN1/SNUPN is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07714-1-snupn-primary-antibodies-ihc-testing-1.jpgAnti-SNURPORTIN1/SNUPN Antibody Picoband®IHC analysis of SNUPN using anti-SNUPN antibody (A07714-1). SNUPN was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNUPN Antibody (A07714-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07714-1-snupn-primary-antibodies-if-testing-2.jpgAnti-SNURPORTIN1/SNUPN Antibody Picoband® IF analysis of SNURPORTIN1/SNUPN using anti-SNURPORTIN1/SNUPN antibody (A07714-1). SNURPORTIN1/SNUPN was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNURPORTIN1/SNUPN Antibody (A07714-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07714-1-snupn-primary-antibodies-fcm-testing-3.pngAnti-SNURPORTIN1/SNUPN Antibody Picoband® Flow Cytometry analysis of JK cells using anti-SNURPORTIN1/SNUPN antibody (A07714-1). Overlay histogram showing JK cells stained with A07714-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNURPORTIN1/SNUPN Antibody (A07714-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snx1-picoband-trade-antibody-a02692-2-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02692-2-snx1-primary-antibodies-wb-testing-1.jpgAnti-SNX1 Antibody Picoband® Western blot analysis of SNX1 using anti-SNX1 antibody (A02692-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX1 antigen affinity purified polyclonal antibody (Catalog # A02692-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX1 at approximately 70-75 kDa. The expected band size for SNX1 is at 59 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02692-2-snx1-primary-antibodies-ihc-testing-2.jpgAnti-SNX1 Antibody Picoband® IHC analysis of SNX1 using anti-SNX1 antibody (A02692-2). SNX1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX1 Antibody (A02692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02692-2-snx1-primary-antibodies-ihc-testing-3.jpgAnti-SNX1 Antibody Picoband® IHC analysis of SNX1 using anti-SNX1 antibody (A02692-2). SNX1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX1 Antibody (A02692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02692-2-snx1-primary-antibodies-ihc-testing-4.jpgAnti-SNX1 Antibody Picoband® IHC analysis of SNX1 using anti-SNX1 antibody (A02692-2). SNX1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX1 Antibody (A02692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02692-2-snx1-primary-antibodies-fcm-testing-6.pngAnti-SNX1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SNX1 antibody (A02692-2). Overlay histogram showing U20S cells stained with A02692-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX1 Antibody (A02692-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02692-2-snx1-primary-antibodies-if-testing-5.jpgAnti-SNX1 Antibody Picoband® IF analysis of SNX1 using anti-SNX1 antibody (A02692-2). SNX1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNX1 Antibody (A02692-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-snx4-picoband-trade-antibody-a08783-2-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08783-2-snx4-primary-antibodies-wb-testing-1.jpgAnti-SNX4 Antibody Picoband® Western blot analysis of SNX4 using anti-SNX4 antibody (A08783-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX4 antigen affinity purified polyclonal antibody (Catalog # A08783-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX4 at approximately 55 kDa. The expected band size for SNX4 is at 52 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08783-2-snx4-primary-antibodies-ihc-testing-2.jpgAnti-SNX4 Antibody Picoband® IHC analysis of SNX4 using anti-SNX4 antibody (A08783-2). SNX4 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX4 Antibody (A08783-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08783-2-snx4-primary-antibodies-ihc-testing-3.jpgAnti-SNX4 Antibody Picoband® IHC analysis of SNX4 using anti-SNX4 antibody (A08783-2). SNX4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX4 Antibody (A08783-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08783-2-snx4-primary-antibodies-ihc-testing-4.jpgAnti-SNX4 Antibody Picoband® IHC analysis of SNX4 using anti-SNX4 antibody (A08783-2). SNX4 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX4 Antibody (A08783-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08783-2-snx4-primary-antibodies-ihc-testing-5.jpgAnti-SNX4 Antibody Picoband® IHC analysis of SNX4 using anti-SNX4 antibody (A08783-2). SNX4 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX4 Antibody (A08783-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-snx5-picoband-trade-antibody-a04788-1-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04788-1-snx5-primary-antibodies-wb-testing-1.jpgAnti-SNX5 Antibody Picoband® Western blot analysis of SNX5 using anti-SNX5 antibody (A04788-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX5 antigen affinity purified polyclonal antibody (Catalog # A04788-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX5 at approximately 47 kDa. The expected band size for SNX5 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04788-1-snx5-primary-antibodies-fcm-testing-2.pngAnti-SNX5 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-SNX5 antibody (A04788-1). Overlay histogram showing U20S cells stained with A04788-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX5 Antibody (A04788-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sh3px1-snx9-picoband-trade-antibody-a03796-2-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03796-2-snx9-primary-antibodies-wb-testing-1.jpgAnti-SH3PX1/SNX9 Antibody Picoband® Western blot analysis of SH3PX1/SNX9 using anti-SH3PX1/SNX9 antibody (A03796-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3PX1/SNX9 antigen affinity purified polyclonal antibody (Catalog # A03796-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3PX1/SNX9 at approximately 78 kDa. The expected band size for SH3PX1/SNX9 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03796-2-snx9-primary-antibodies-ihc-testing-1.jpgAnti-SH3PX1/SNX9 Antibody Picoband®IHC analysis of SNX9 using anti-SNX9 antibody (A03796-2). SNX9 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX9 Antibody (A03796-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03796-2-snx9-primary-antibodies-if-testing-1.jpgAnti-SH3PX1/SNX9 Antibody Picoband®IF analysis of SNX9 using anti-SNX9 antibody (A03796-2). SNX9 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNX9 Antibody (A03796-2) overnight at 4°C. Cy Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03796-2-snx9-primary-antibodies-fcm-testing-2.pngAnti-SH3PX1/SNX9 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-SH3PX1/SNX9 antibody (A03796-2). Overlay histogram showing MCF-7 cells stained with A03796-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH3PX1/SNX9 Antibody (A03796-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snx15-picoband-trade-antibody-a11463-2-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11463-2-snx15-primary-antibodies-wb-testing-1.jpgAnti-SNX15 Antibody Picoband® Western blot analysis of SNX15 using anti-SNX15 antibody (A11463-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX15 antigen affinity purified polyclonal antibody (Catalog # A11463-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX15 at approximately 50 kDa. The expected band size for SNX15 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-snx18-picoband-trade-antibody-a09010-2-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09010-2-snx18-primary-antibodies-wb-testing-1.jpgAnti-SNX18 Antibody Picoband® Western blot analysis of SNX18 using anti-SNX18 antibody (A09010-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat lung tisue lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX18 antigen affinity purified polyclonal antibody (Catalog # A09010-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX18 at approximately 69 kDa. The expected band size for SNX18 is at 69 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-snx30-picoband-trade-antibody-a14226-1-boster.html2026-03-17T05:14:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14226-1-snx30-primary-antibodies-wb-testing-1.jpgAnti-SNX30 Antibody Picoband® Western blot analysis of SNX30 using anti-SNX30 antibody (A14226-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX30 antigen affinity purified polyclonal antibody (Catalog # A14226-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX30 at approximately 50 kDa. The expected band size for SNX30 is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-snx31-picoband-trade-antibody-a14470-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14470-1-snx31-primary-antibodies-wb-testing-1.jpgAnti-SNX31 Antibody Picoband® Western blot analysis of SNX31 using anti-SNX31 antibody (A14470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX31 antigen affinity purified polyclonal antibody (Catalog # A14470-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX31 at approximately 51 kDa. The expected band size for SNX31 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14470-1-snx31-primary-antibodies-fcm-testing-2.pngAnti-SNX31 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-SNX31 antibody (A14470-1). Overlay histogram showing U251 cells stained with A14470-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX31 Antibody (A14470-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-syntaxin-6-stx6-picoband-trade-antibody-a06586-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06586-1-stx6-primary-antibodies-wb-testing-1.jpgAnti-Syntaxin 6/STX6 Antibody Picoband® Western blot analysis of Syntaxin 6/STX6 using anti-Syntaxin 6/STX6 antibody (A06586-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 6/STX6 antigen affinity purified polyclonal antibody (Catalog # A06586-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 6/STX6 at approximately 29 kDa. The expected band size for Syntaxin 6/STX6 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06586-1-stx6-primary-antibodies-fcm-testing-3.pngAnti-Syntaxin 6/STX6 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-Syntaxin 6/STX6 antibody (A06586-1). Overlay histogram showing U20S cells stained with A06586-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 6/STX6 Antibody (A06586-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tsen34-picoband-trade-antibody-a12386-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12386-1-tsen34-primary-antibodies-wb-testing-1.jpgAnti-TSEN34 Antibody Picoband® Western blot analysis of TSEN34 using anti-TSEN34 antibody (A12386-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSEN34 antigen affinity purified polyclonal antibody (Catalog # A12386-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSEN34 at approximately 50 kDa. The expected band size for TSEN34 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12386-1-tsen34-primary-antibodies-if-testing-2.jpgAnti-TSEN34 Antibody Picoband® IF analysis of TSEN34 and Tubulin beta using anti-TSEN34 antibody (A12386-1) and anti-Tubulin beta antibody (M05613-4). TSEN34 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSEN34 Antibody (A12386-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tst-picoband-trade-antibody-a00965-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-wb-testing-1.jpgAnti-TST Antibody Picoband® Western blot analysis of TST using anti-TST antibody (A00965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TST antigen affinity purified polyclonal antibody (Catalog # A00965-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TST at approximately 34 kDa. The expected band size for TST is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-2.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-3.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-4.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-5.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-6.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-7.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-8.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-9.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human right renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-10.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-ihc-testing-11.jpgAnti-TST Antibody Picoband® IHC analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-if-testing-12.jpgAnti-TST Antibody Picoband® IF analysis of TST using anti-TST antibody (A00965-1). TST was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TST Antibody (A00965-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00965-1-tst-primary-antibodies-if-testing-13_1.jpgAnti-TST Antibody Picoband® IF analysis of TST using anti-TST antibody (A00965-1). TST was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TST Antibody (A00965-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-txndc5-picoband-trade-antibody-a05772-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-wb-testing-1.jpgAnti-TXNDC5 Antibody Picoband® Western blot analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNDC5 antigen affinity purified polyclonal antibody (Catalog # A05772-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNDC5 at approximately 50-55 kDa. The expected band size for TXNDC5 is at 48 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-2.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-3.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-4.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-5.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-6.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-7.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-8.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-9.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-ihc-testing-10.jpgAnti-TXNDC5 Antibody Picoband® IHC analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-if-testing-11.jpgAnti-TXNDC5 Antibody Picoband® IF analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). TXNDC5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TXNDC5 Antibody (A05772-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05772-1-txndc5-primary-antibodies-fcm-testing-12.jpgAnti-TXNDC5 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TXNDC5 antibody (A05772-1). Overlay histogram showing U87 cells stained with A05772-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXNDC5 Antibody (A05772-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/picokine-elisa-kits/human-ggct-crf21-picokine-elisa-kit-ek2205-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2205.pngHuman GGCT/CRF21 ELISA Kit PicoKine®Human GGCT PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gla-picokine-elisa-kit-ek2206-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2206.jpgHuman GLA ELISA Kit PicoKine®Human GLA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-nepmucin-cd300g-picokine-elisa-kit-ek2207-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2207.pngMouse Nepmucin/CD300g ELISA Kit PicoKine®Mouse Nepmucin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-glb1-picokine-elisa-kit-ek2208-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2208.jpgHuman GLB1 ELISA Kit PicoKine®Human GLB1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-glo1-picokine-elisa-kit-ek2209-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2209.jpgHuman GLO1 ELISA Kit PicoKine®Human GLO1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd42b-picokine-elisa-kit-ek2210-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2210.pngHuman CD42b ELISA Kit PicoKine®Human CD42b PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd42d-picokine-elisa-kit-ek2211-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2211.pngHuman CD42d ELISA Kit PicoKine®Human CD42d PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gpvi-picokine-elisa-kit-ek2212-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2212.pngHuman GPVI ELISA Kit PicoKine®Human GPVI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gstm1-picokine-elisa-kit-ek2213-boster.html2026-03-10T04:35:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2213.pngHuman GSTM1 ELISA Kit PicoKine®Human GSTM1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-fancc-picoband-trade-antibody-a02387-2-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02387-2-fancc-primary-antibodies-wb-testing-1.jpgAnti-FANCC Antibody Picoband® Western blot analysis of FANCC using anti-FANCC antibody (A02387-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCC antigen affinity purified polyclonal antibody (Catalog # A02387-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FANCC at approximately 63 kDa. The expected band size for FANCC is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02387-2-fancc-primary-antibodies-fcm-testing-2.jpgAnti-FANCC Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-FANCC antibody (A02387-2). Overlay histogram showing U20S cells stained with A02387-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FANCC Antibody (A02387-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-snx6-picoband-trade-antibody-a06545-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06545-snx6-primary-antibodies-wb-testing-1.jpgAnti-SNX6 Antibody Picoband® Western blot analysis of SNX6 using anti-SNX6 antibody (A06545). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX6 antigen affinity purified polyclonal antibody (Catalog # A06545) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX6 at approximately 47 kDa. The expected band size for SNX6 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06545-snx6-primary-antibodies-ihc-testing-1.jpgAnti-SNX6 Antibody Picoband®IHC analysis of SNX6 using anti-SNX6 antibody (A06545). SNX6 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX6 Antibody (A06545) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06545-snx6-primary-antibodies-if-testing-2.jpgAnti-SNX6 Antibody Picoband® IF analysis of SNX6 using anti-SNX6 antibody (A06545). SNX6 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNX6 Antibody (A06545) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06545-snx6-primary-antibodies-ip-testing-1.jpgAnti-SNX6 Antibody Picoband®Immunoprecipitating (IP) SNX6 in A549 whole cell lysate. Western blot analysis of SNX6 using anti-SNX6 antibody (A06545); Lane 1: A549 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SNX6 antibody in A549 whole cell lysate; Lane 3: anti-SNX6 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNX6 antigen affinity purified polyclonal antibody (A06545) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SNX6 at approximately 47 kDa. The expected band size for SNX6 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06545-snx6-primary-antibodies-fcm-testing-3.jpgAnti-SNX6 Antibody Picoband® Flow Cytometry analysis of HCT116 cells using anti-SNX6 antibody (A06545). Overlay histogram showing HCT116 cells stained with A06545 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX6 Antibody (A06545, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dna-ligase-iv-lig4-picoband-trade-antibody-a01685-3-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01685-3-lig4-primary-antibodies-wb-testing-1_1.jpgAnti-DNA Ligase IV/LIG4 Antibody Picoband® Western blot analysis of LIG4 using anti-LIG4 antibody (A01685-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human jurkat whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIG4 antigen affinity purified polyclonal antibody (A01685-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LIG4 at approximately 104 kDa. The expected band size for LIG4 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01685-3-lig4-primary-antibodies-if-testing-2_1.jpgAnti-DNA Ligase IV/LIG4 Antibody Picoband® IF analysis of DNA Ligase IV/LIG4 using anti-DNA Ligase IV/LIG4 antibody (A01685-3) and anti-Tubulin Alpha antibody (M03989-3). DNA Ligase IV/LIG4 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DNA Ligase IV/LIG4 Antibody (A01685-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01685-3-lig4-primary-antibodies-fcm-testing-3.pngAnti-DNA Ligase IV/LIG4 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-LIG4 antibody (A01685-3). Overlay histogram showing Jurkat cells stained with A01685-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LIG4 Antibody (A04887-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spata5-picoband-trade-antibody-a06364-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06364-1-spata5-primary-antibodies-wb-testing-1.jpgAnti-SPATA5 Antibody Picoband® Western blot analysis of SPATA5 using anti-SPATA5 antibody (A06364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA5 antigen affinity purified polyclonal antibody (Catalog # A06364-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA5 at approximately 98 kDa. The expected band size for SPATA5 is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06364-1-spata5-primary-antibodies-fcm-testing-2.jpgAnti-SPATA5 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SPATA5 antibody (A06364-1). Overlay histogram showing HepG2 cells stained with A06364-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA5 Antibody (A06364-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-specc1l-picoband-trade-antibody-a09120-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09120-1-specc1l-primary-antibodies-wb-testing-1_1.jpgAnti-SPECC1L Antibody Picoband® Western blot analysis of SPECC1L using anti-SPECC1L antibody (A09120-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPECC1L antigen affinity purified polyclonal antibody (Catalog # A09120-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPECC1L at approximately 150 kDa. The expected band size for SPECC1L is at 125 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09120-1-specc1l-primary-antibodies-if-testing-2.jpgAnti-SPECC1L Antibody Picoband® IF analysis of SPECC1L using anti-SPECC1L antibody (A09120-1). SPECC1L was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPECC1L Antibody (A09120-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09120-1-specc1l-primary-antibodies-fcm-testing-3.jpgAnti-SPECC1L Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SPECC1L antibody (A09120-1). Overlay histogram showing HepG2 cells stained with A09120-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPECC1L Antibody (A09120-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spag7-picoband-trade-antibody-a14828-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14828-1-spag7-primary-antibodies-wb-testing-1.jpgAnti-SPAG7 Antibody Picoband® Western blot analysis of SPAG7 using anti-SPAG7 antibody (A14828-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPAG7 antigen affinity purified polyclonal antibody (Catalog # A14828-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPAG7 at approximately 35 kDa. The expected band size for SPAG7 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14828-1-spag7-primary-antibodies-ihc-testing-1.jpgAnti-SPAG7 Antibody Picoband®IHC analysis of SPAG7 using anti-SPAG7 antibody (A14828-1). SPAG7 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPAG7 Antibody (A14828-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14828-1-spag7-primary-antibodies-if-testing-2.jpgAnti-SPAG7 Antibody Picoband® IF analysis of SPAG7 using anti-SPAG7 antibody (A14828-1) and anti-Tubulin Alpha antibody (M03989-3). SPAG7 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPAG7 Antibody (A14828-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14828-1-spag7-primary-antibodies-fcm-testing-3.jpgAnti-SPAG7 Antibody Picoband® Flow Cytometry analysis of HCT116 cells using anti-SPAG7 antibody (A14828-1). Overlay histogram showing HCT116 cells stained with A14828-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPAG7 Antibody (A14828-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14828-1-spag7-primary-antibodies-fcm-testing-4.jpgAnti-SPAG7 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SPAG7 antibody (A14828-1). Overlay histogram showing PC-3 cells stained with A14828-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPAG7 Antibody (A14828-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spink13-picoband-trade-antibody-a18284-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18284-spink13-primary-antibodies-wb-testing-1.jpgAnti-SPINK13 Antibody Picoband® Western blot analysis of SPINK13 using anti-SPINK13 antibody (A18284). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPINK13 antigen affinity purified polyclonal antibody (Catalog # A18284) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPINK13 at approximately 24 kDa. The expected band size for SPINK13 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18284-spink13-primary-antibodies-fcm-testing-2.jpgAnti-SPINK13 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-SPINK13 antibody (A18284). Overlay histogram showing Hela cells stained with A18284 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPINK13 Antibody (A18284, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18284-spink13-primary-antibodies-fcm-testing-3.jpgAnti-SPINK13 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SPINK13 antibody (A18284). Overlay histogram showing SiHa cells stained with A18284 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPINK13 Antibody (A18284, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spata7-picoband-trade-antibody-a08310-1-boster.html2026-03-17T05:14:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08310-1-spata7-primary-antibodies-wb-testing-1.jpgAnti-SPATA7 Antibody Picoband® Western blot analysis of SPATA7 using anti-SPATA7 antibody (A08310-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA7 antigen affinity purified polyclonal antibody (Catalog # A08310-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA7 at approximately 68 kDa. The expected band size for SPATA7 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08310-1-spata7-primary-antibodies-fcm-testing-2.jpgAnti-SPATA7 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SPATA7 antibody (A08310-1). Overlay histogram showing HepG2 cells stained with A08310-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA7 Antibody (A08310-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spata18-picoband-trade-antibody-a09906-1-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09906-1-spata18-primary-antibodies-wb-testing-1.jpgAnti-SPATA18 Antibody Picoband® Western blot analysis of SPATA18 using anti-SPATA18 antibody (A09906-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA18 antigen affinity purified polyclonal antibody (Catalog # A09906-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA18 at approximately 61 kDa. The expected band size for SPATA18 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09906-1-spata18-primary-antibodies-ihc-testing-2.jpgAnti-SPATA18 Antibody Picoband® IHC analysis of SPATA18 using anti-SPATA18 antibody (A09906-1). SPATA18 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPATA18 Antibody (A09906-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09906-1-spata18-primary-antibodies-ihc-testing-3.jpgAnti-SPATA18 Antibody Picoband® IHC analysis of SPATA18 using anti-SPATA18 antibody (A09906-1). SPATA18 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPATA18 Antibody (A09906-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09906-1-spata18-primary-antibodies-ihc-testing-4.jpgAnti-SPATA18 Antibody Picoband® IHC analysis of SPATA18 using anti-SPATA18 antibody (A09906-1). SPATA18 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPATA18 Antibody (A09906-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09906-1-spata18-primary-antibodies-fcm-testing-5.jpgAnti-SPATA18 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SPATA18 antibody (A09906-1). Overlay histogram showing HepG2 cells stained with A09906-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA18 Antibody (A09906-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spata20-picoband-trade-antibody-a17005-1-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17005-1-spata20-primary-antibodies-wb-testing-1.jpgAnti-SPATA20 Antibody Picoband® Western blot analysis of SPATA20 using anti-SPATA20 antibody (A17005-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA20 antigen affinity purified polyclonal antibody (Catalog # A17005-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA20 at approximately 88 kDa. The expected band size for SPATA20 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17005-1-spata20-primary-antibodies-fcm-testing-2.jpgAnti-SPATA20 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SPATA20 antibody (A17005-1). Overlay histogram showing 293T cells stained with A17005-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA20 Antibody (A17005-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pse-spdef-picoband-trade-antibody-a04625-1-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04625-1-spdef-primary-antibodies-wb-testing-1.jpgAnti-PSE/SPDEF Antibody Picoband® Western blot analysis of PSE/SPDEF using anti-PSE/SPDEF antibody (A04625-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSE/SPDEF antigen affinity purified polyclonal antibody (Catalog # A04625-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSE/SPDEF at approximately 44 kDa. The expected band size for PSE/SPDEF is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04625-1-spdef-primary-antibodies-if-testing-2.jpgAnti-PSE/SPDEF Antibody Picoband® IF analysis of PSE/SPDEF using anti-PSE/SPDEF antibody (A04625-1) and anti-Tubulin Alpha antibody (M03989-3). PSE/SPDEF was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSE/SPDEF Antibody (A04625-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04625-1-spdef-primary-antibodies-fcm-testing-3.jpgAnti-PSE/SPDEF Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PSE/SPDEF antibody (A04625-1). Overlay histogram showing MCF-7 cells stained with A04625-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSE/SPDEF Antibody (A04625-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04625-1-spdef-primary-antibodies-fcm-testing-4.jpgAnti-PSE/SPDEF Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-PSE/SPDEF antibody (A04625-1). Overlay histogram showing PC-3 cells stained with A04625-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSE/SPDEF Antibody (A04625-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spindly-spdl1-picoband-trade-antibody-a12722-2-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12722-2-spdl1-primary-antibodies-wb-testing-1.jpgAnti-Spindly/SPDL1 Antibody Picoband® Western blot analysis of Spindly/SPDL1 using anti-Spindly/SPDL1 antibody (A12722-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spindly/SPDL1 antigen affinity purified polyclonal antibody (Catalog # A12722-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spindly/SPDL1 at approximately 70 kDa. The expected band size for Spindly/SPDL1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12722-2-spdl1-primary-antibodies-if-testing-2.jpgAnti-Spindly/SPDL1 Antibody Picoband® IF analysis of Spindly/SPDL1 using anti-Spindly/SPDL1 antibody (A12722-2). Spindly/SPDL1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Spindly/SPDL1 Antibody (A12722-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12722-2-spdl1-primary-antibodies-fcm-testing-3.jpgAnti-Spindly/SPDL1 Antibody Picoband® Flow Cytometry analysis of HCT116 cells using anti-Spindly/SPDL1 antibody (A12722-2). Overlay histogram showing HCT116 cells stained with A12722-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Spindly/SPDL1 Antibody (A12722-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12722-2-spdl1-primary-antibodies-fcm-testing-4.jpgAnti-Spindly/SPDL1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-Spindly/SPDL1 antibody (A12722-2). Overlay histogram showing PC-3 cells stained with A12722-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Spindly/SPDL1 Antibody (A12722-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spon2-picoband-trade-antibody-a07465-1-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07465-1-spon2-primary-antibodies-wb-testing-1.jpgAnti-SPON2 Antibody Picoband® Western blot analysis of SPON2 using anti-SPON2 antibody (A07465-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPON2 antigen affinity purified polyclonal antibody (Catalog # A07465-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPON2 at approximately 39 kDa. The expected band size for SPON2 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pctp-l-stard10-picoband-trade-antibody-a10577-1-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10577-1-stard10-primary-antibodies-ihc-testing-1.jpgAnti-PCTP-L/STARD10 Antibody Picoband®IHC analysis of STARD10 using anti-STARD10 antibody (A10577-1). STARD10 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STARD10 Antibody (A10577-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10577-1-stard10-primary-antibodies-wb-testing-1_1.jpgAnti-PCTP-L/STARD10 Antibody Picoband® Western blot analysis of PCTP-L/STARD10 using anti-PCTP-L/STARD10 antibody (A10577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCTP-L/STARD10 antigen affinity purified polyclonal antibody (Catalog # A10577-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCTP-L/STARD10 at approximately 37 kDa. The expected band size for PCTP-L/STARD10 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10577-1-stard10-primary-antibodies-if-testing-2.jpgAnti-PCTP-L/STARD10 Antibody Picoband® IF analysis of PCTP-L/STARD10 using anti-PCTP-L/STARD10 antibody (A10577-1). PCTP-L/STARD10 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PCTP-L/STARD10 Antibody (A10577-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10577-1-stard10-primary-antibodies-fcm-testing-3.jpgAnti-PCTP-L/STARD10 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PCTP-L/STARD10 antibody (A10577-1). Overlay histogram showing MCF-7 cells stained with A10577-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCTP-L/STARD10 Antibody (A10577-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-stat1-picoband-trade-antibody-a00036-3-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00036-3-stat1-primary-antibodies-wb-testing-1.jpgAnti-STAT1 Antibody Picoband® Western blot analysis of STAT1 using anti-STAT1 antibody (A00036-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # A00036-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00036-3-stat1-primary-antibodies-wb-testing-2.jpgAnti-STAT1 Antibody Picoband® Western blot analysis of STAT1 using anti-STAT1 antibody (A00036-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T- WT whole cell lysates, Lane 2: human 293T-STAT1 KO whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (A00036-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00036-3-stat1-primary-antibodies-fcm-testing-3.jpgAnti-STAT1 Antibody Picoband® Flow Cytometry analysis of HCT116 cells using anti-STAT1 antibody (A00036-3). Overlay histogram showing HCT116 cells stained with A00036-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (A00036-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-srprb-picoband-trade-antibody-a13302-1-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13302-1-srprb-primary-antibodies-wb-testing-1.jpgAnti-SRPRB Antibody Picoband® Western blot analysis of SRPRB using anti-SRPRB antibody (A13302-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRB antigen affinity purified polyclonal antibody (Catalog # A13302-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRPRB at approximately 30 kDa. The expected band size for SRPRB is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13302-1-srprb-primary-antibodies-ihc-testing-2.jpgAnti-SRPRB Antibody Picoband® IHC analysis of SRPRB using anti-SRPRB antibody (A13302-1). SRPRB was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRPRB Antibody (A13302-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13302-1-srprb-primary-antibodies-ihc-testing-3.jpgAnti-SRPRB Antibody Picoband® IHC analysis of SRPRB using anti-SRPRB antibody (A13302-1). SRPRB was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRPRB Antibody (A13302-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13302-1-srprb-primary-antibodies-if-testing-4.jpgAnti-SRPRB Antibody Picoband® IF analysis of SRPRB using anti-SRPRB antibody (A13302-1). SRPRB was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SRPRB Antibody (A13302-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13302-1-srprb-primary-antibodies-if-testing-5.jpgAnti-SRPRB Antibody Picoband® IF analysis of SRPRB using anti-SRPRB antibody (A13302-1). SRPRB was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SRPRB Antibody (A13302-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13302-1-srprb-primary-antibodies-fcm-testing-6.jpgAnti-SRPRB Antibody Picoband® Flow Cytometry analysis of HCT116 cells using anti-SRPRB antibody (A13302-1). Overlay histogram showing HCT116 cells stained with A13302-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRB Antibody (A13302-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-srpra-picoband-trade-antibody-a12340-1-boster.html2026-03-17T05:14:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12340-1-srpra-primary-antibodies-wb-testing-1.jpgAnti-SRPRA Antibody Picoband® Western blot analysis of SRPRA using anti-SRPRA antibody (A12340-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRA antigen affinity purified polyclonal antibody (Catalog # A12340-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRPRA at approximately 70 kDa. The expected band size for SRPRA is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12340-1-srpra-primary-antibodies-ihc-testing-2.jpgAnti-SRPRA Antibody Picoband® IHC analysis of SRPRA using anti-SRPRA antibody (A12340-1). SRPRA was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRPRA Antibody (A12340-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12340-1-srpra-primary-antibodies-ihc-testing-3.jpgAnti-SRPRA Antibody Picoband® IHC analysis of SRPRA using anti-SRPRA antibody (A12340-1). SRPRA was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRPRA Antibody (A12340-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12340-1-srpra-primary-antibodies-if-testing-4.jpgAnti-SRPRA Antibody Picoband® IF analysis of SRPRA using anti-SRPRA antibody (A12340-1). SRPRA was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SRPRA Antibody (A12340-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12340-1-srpra-primary-antibodies-if-testing-5.jpgAnti-SRPRA Antibody Picoband® IF analysis of SRPRA using anti-SRPRA antibody (A12340-1). SRPRA was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SRPRA Antibody (A12340-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12340-1-srpra-primary-antibodies-fcm-testing-6.jpgAnti-SRPRA Antibody Picoband® Flow Cytometry analysis of HCT116 cells using anti-SRPRA antibody (A12340-1). Overlay histogram showing HCT116 cells stained with A12340-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRA Antibody (A12340-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-st13-picoband-trade-antibody-a05692-1-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05692-1-st13-primary-antibodies-wb-testing-1.jpgAnti-ST13 Antibody Picoband® Western blot analysis of ST13 using anti-ST13 antibody (A05692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST13 antigen affinity purified polyclonal antibody (Catalog # A05692-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST13 at approximately 45-54 kDa. The expected band size for ST13 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05692-1-st13-primary-antibodies-if-testing-2.jpgAnti-ST13 Antibody Picoband® IF analysis of ST13 using anti-ST13 antibody (A05692-1). ST13 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ST13 Antibody (A05692-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05692-1-st13-primary-antibodies-fcm-testing-3.jpgAnti-ST13 Antibody Picoband® Flow Cytometry analysis of HCT116 cells using anti-ST13 antibody (A05692-1). Overlay histogram showing HCT116 cells stained with A05692-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ST13 Antibody (A05692-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-stbd1-picoband-trade-antibody-a10113-1-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10113-1-stbd1-primary-antibodies-wb-testing-1.jpgAnti-STBD1 Antibody Picoband® Western blot analysis of STBD1 using anti-STBD1 antibody (A10113-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STBD1 antigen affinity purified polyclonal antibody (Catalog # A10113-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STBD1 at approximately 39 kDa. The expected band size for STBD1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10113-1-stbd1-primary-antibodies-ihc-testing-2.jpgAnti-STBD1 Antibody Picoband® IHC analysis of STBD1 using anti-STBD1 antibody (A10113-1). STBD1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STBD1 Antibody (A10113-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10113-1-stbd1-primary-antibodies-ihc-testing-3.jpgAnti-STBD1 Antibody Picoband® IHC analysis of STBD1 using anti-STBD1 antibody (A10113-1). STBD1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STBD1 Antibody (A10113-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10113-1-stbd1-primary-antibodies-ihc-testing-4.jpgAnti-STBD1 Antibody Picoband® IHC analysis of STBD1 using anti-STBD1 antibody (A10113-1). STBD1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STBD1 Antibody (A10113-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10113-1-stbd1-primary-antibodies-if-testing-5.jpgAnti-STBD1 Antibody Picoband® IF analysis of STBD1 using anti-STBD1 antibody (A10113-1). STBD1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STBD1 Antibody (A10113-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10113-1-stbd1-primary-antibodies-fcm-testing-6.jpgAnti-STBD1 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-STBD1 antibody (A10113-1). Overlay histogram showing PC-3 cells stained with A10113-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STBD1 Antibody (A10113-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ston2-picoband-trade-antibody-a10624-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10624-ston2-primary-antibodies-wb-testing-1_1.jpgAnti-STON2 Antibody Picoband®Western blot analysis of STON2 using anti-STON2 antibody (A10624). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STON2 antigen affinity purified polyclonal antibody (A10624) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STON2 at approximately 130-150 kDa. The expected band size for STON2 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10624-ston2-primary-antibodies-ihc-testing-1_1.jpgAnti-STON2 Antibody Picoband®IHC analysis of STON2 using anti-STON2 antibody (A10624). STON2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON2 Antibody (A10624) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10624-ston2-primary-antibodies-ihc-testing-2.jpgAnti-STON2 Antibody Picoband®IHC analysis of STON2 using anti-STON2 antibody (A10624). STON2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON2 Antibody (A10624) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10624-ston2-primary-antibodies-ihc-testing-3.jpgAnti-STON2 Antibody Picoband®IHC analysis of STON2 using anti-STON2 antibody (A10624). STON2 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON2 Antibody (A10624) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10624-ston2-primary-antibodies-ihc-testing-4.jpgAnti-STON2 Antibody Picoband®IHC analysis of STON2 using anti-STON2 antibody (A10624). STON2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON2 Antibody (A10624) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10624-ston2-primary-antibodies-if-testing-1.jpgAnti-STON2 Antibody Picoband®IF analysis of STON2 using anti-STON2 antibody (A10624). STON2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STON2 Antibody (A10624) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10624-ston2-primary-antibodies-fcm-testing-1.jpgAnti-STON2 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-STON2 antibody (A10624). Overlay histogram showing HEL cells stained with A10624 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STON2 Antibody (A10624, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spaca3-picoband-trade-antibody-a10284-1-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10284-1-spaca3-primary-antibodies-wb-testing-1.jpgAnti-SPACA3 Antibody Picoband® Western blot analysis of SPACA3 using anti-SPACA3 antibody (A10284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPACA3 antigen affinity purified polyclonal antibody (Catalog # A10284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPACA3 at approximately 40 kDa. The expected band size for SPACA3 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10284-1-spaca3-primary-antibodies-if-testing-2.jpgAnti-SPACA3 Antibody Picoband® IF analysis of SPACA3 using anti-SPACA3 antibody (A10284-1). SPACA3 was detected in an immunocytochemical section of CACO2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPACA3 Antibody (A10284-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10284-1-spaca3-primary-antibodies-fcm-testing-3.jpgAnti-SPACA3 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-SPACA3 antibody (A10284-1). Overlay histogram showing Daudi cells stained with A10284-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPACA3 Antibody (A10284-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spred2-picoband-trade-antibody-a06368-1-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06368-1-spred2-primary-antibodies-wb-testing-1.jpgAnti-SPRED2 Antibody Picoband® Western blot analysis of SPRED2 using anti-SPRED2 antibody (A06368-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPRED2 antigen affinity purified polyclonal antibody (Catalog # A06368-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPRED2 at approximately 53 kDa. The expected band size for SPRED2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06368-1-spred2-primary-antibodies-ihc-testing-1.jpgAnti-SPRED2 Antibody Picoband®IHC analysis of SPRED2 using anti-SPRED2 antibody (A06368-1). SPRED2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPRED2 Antibody (A06368-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06368-1-spred2-primary-antibodies-if-testing-2.jpgAnti-SPRED2 Antibody Picoband® IF analysis of SPRED2 using anti-SPRED2 antibody (A06368-1) and anti-Tubulin Alpha antibody (M03989-3). SPRED2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPRED2 Antibody (A06368-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06368-1-spred2-primary-antibodies-fcm-testing-3.jpgAnti-SPRED2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SPRED2 antibody (A06368-1). Overlay histogram showing HepG2 cells stained with A06368-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPRED2 Antibody (A06368-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-beta-1-spectrin-sptb-picoband-trade-antibody-a02392-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02392-sptb-primary-antibodies-wb-testing-1.jpgAnti-beta 1 Spectrin/SPTB Antibody Picoband® Western blot analysis of beta 1 Spectrin/SPTB using anti-beta 1 Spectrin/SPTB antibody (A02392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta 1 Spectrin/SPTB antigen affinity purified polyclonal antibody (Catalog # A02392) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta 1 Spectrin/SPTB at approximately 270 kDa. The expected band size for beta 1 Spectrin/SPTB is at 246 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02392-sptb-primary-antibodies-fcm-testing-2.jpgAnti-beta 1 Spectrin/SPTB Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-beta 1 Spectrin/SPTB antibody (A02392). Overlay histogram showing U937 cells stained with A02392 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-beta 1 Spectrin/SPTB Antibody (A02392, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sptbn2-picoband-trade-antibody-a05492-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05492-sptbn2-primary-antibodies-wb-testing-1.jpgAnti-SPTBN2 Antibody Picoband® Western blot analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPTBN2 antigen affinity purified polyclonal antibody (Catalog # A05492) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPTBN2 at approximately 271 kDa. The expected band size for SPTBN2 is at 271 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05492-sptbn2-primary-antibodies-ihc-testing-6.jpgAnti-SPTBN2 Antibody Picoband®IHC analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). SPTBN2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPTBN2 Antibody (A05492) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05492-sptbn2-primary-antibodies-ihc-testing-2.jpgAnti-SPTBN2 Antibody Picoband® IHC analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). SPTBN2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPTBN2 Antibody (A05492) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05492-sptbn2-primary-antibodies-ihc-testing-3.jpgAnti-SPTBN2 Antibody Picoband® IHC analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). SPTBN2 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPTBN2 Antibody (A05492) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05492-sptbn2-primary-antibodies-ihc-testing-4.jpgAnti-SPTBN2 Antibody Picoband® IHC analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). SPTBN2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPTBN2 Antibody (A05492) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05492-sptbn2-primary-antibodies-ihc-testing-5.jpgAnti-SPTBN2 Antibody Picoband® IHC analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). SPTBN2 was detected in a paraffin-embedded section of rat skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPTBN2 Antibody (A05492) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05492-sptbn2-primary-antibodies-fcm-testing-6.jpgAnti-SPTBN2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SPTBN2 antibody (A05492). Overlay histogram showing Jurkat cells stained with A05492 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPTBN2 Antibody (A05492, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hfe-picoband-trade-antibody-a00506-5-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00506-5-hfe-primary-antibodies-wb-testing-1.jpgAnti-Hfe Antibody Picoband® Western blot analysis of Hfe using anti-Hfe antibody (A00506-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hfe antigen affinity purified polyclonal antibody (Catalog # A00506-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hfe at approximately 40-58 kDa. The expected band size for Hfe is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00506-5-hfe-primary-antibodies-ihc-testing-2.jpgAnti-Hfe Antibody Picoband® IHC analysis of Hfe using anti-Hfe antibody (A00506-5). Hfe was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hfe Antibody (A00506-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00506-5-hfe-primary-antibodies-fcm-testing-3.jpgAnti-Hfe Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Hfe antibody (A00506-5). Overlay histogram showing HEPA1-6 cells stained with A00506-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Hfe Antibody (A00506-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-srgap3-picoband-trade-antibody-a06020-1-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06020-1-srgap3-primary-antibodies-wb-testing-1.jpgAnti-SRGAP3 Antibody Picoband® Western blot analysis of SRGAP3 using anti-SRGAP3 antibody (A06020-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRGAP3 antigen affinity purified polyclonal antibody (Catalog # A06020-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRGAP3 at approximately 140 kDa. The expected band size for SRGAP3 is at 125 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06020-1-srgap3-primary-antibodies-ihc-testing-4.jpgAnti-SRGAP3 Antibody Picoband®IHC analysis of SRGAP3 using anti-SRGAP3 antibody (A06020-1). SRGAP3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRGAP3 Antibody (A06020-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06020-1-srgap3-primary-antibodies-ihc-testing-2.jpgAnti-SRGAP3 Antibody Picoband® IHC analysis of SRGAP3 using anti-SRGAP3 antibody (A06020-1). SRGAP3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRGAP3 Antibody (A06020-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06020-1-srgap3-primary-antibodies-ihc-testing-3.jpgAnti-SRGAP3 Antibody Picoband® IHC analysis of SRGAP3 using anti-SRGAP3 antibody (A06020-1). SRGAP3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRGAP3 Antibody (A06020-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-srp14-picoband-trade-antibody-a07709-1-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07709-1-srp14-primary-antibodies-wb-testing-1.jpgAnti-SRP14 Antibody Picoband® Western blot analysis of SRP14 using anti-SRP14 antibody (A07709-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRP14 antigen affinity purified polyclonal antibody (Catalog # A07709-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRP14 at approximately 18 kDa. The expected band size for SRP14 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07709-1-srp14-primary-antibodies-ip-testing-1.jpgAnti-SRP14 Antibody Picoband®Immunoprecipitating (IP) SRP14 in PC-3 whole cell lysate. Western blot analysis of SRP14 using anti-SRP14 antibody (A07709-1); Lane 1: PC-3 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SRP14 antibody in PC-3 whole cell lysate; Lane 3: anti-SRP14 antibody (2μg) + PC-3 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SRP14 antigen affinity purified polyclonal antibody (A07709-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SRP14 at approximately 18 kDa. The expected band size for SRP14 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07709-1-srp14-primary-antibodies-fcm-testing-2.jpgAnti-SRP14 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SRP14 antibody (A07709-1). Overlay histogram showing 293T cells stained with A07709-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRP14 Antibody (A07709-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-st8sia2-picoband-trade-antibody-a05894-1-boster.html2026-03-17T05:14:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05894-1-st8sia2-primary-antibodies-wb-testing-1.jpgAnti-ST8SIA2 Antibody Picoband® Western blot analysis of ST8SIA2 using anti-ST8SIA2 antibody (A05894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST8SIA2 antigen affinity purified polyclonal antibody (Catalog # A05894-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST8SIA2 at approximately 39 kDa. The expected band size for ST8SIA2 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sa1-stag1-picoband-trade-antibody-a06471-1-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06471-1-stag1-primary-antibodies-wb-testing-1.jpgAnti-SA1/STAG1 Antibody Picoband® Western blot analysis of SA1/STAG1 using anti-SA1/STAG1 antibody (A06471-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA1/STAG1 antigen affinity purified polyclonal antibody (Catalog # A06471-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SA1/STAG1 at approximately 155 kDa. The expected band size for SA1/STAG1 is at 144 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06471-1-stag1-primary-antibodies-if-testing-2.jpgAnti-SA1/STAG1 Antibody Picoband® IF analysis of SA1/STAG1 using anti-SA1/STAG1 antibody (A06471-1) and anti-Tubulin Alpha antibody (M03989-3). SA1/STAG1 was detected in immunocytochemical section of T47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SA1/STAG1 Antibody (A06471-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06471-1-stag1-primary-antibodies-fcm-testing-3.jpgAnti-SA1/STAG1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-SA1/STAG1 antibody (A06471-1). Overlay histogram showing U937 cells stained with A06471-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SA1/STAG1 Antibody (A06471-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-amsh-lp-stambpl1-picoband-trade-antibody-a09862-1-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09862-1-stambpl1-primary-antibodies-wb-testing-1.jpgAnti-AMSH-LP/STAMBPL1 Antibody Picoband® Western blot analysis of AMSH-LP/STAMBPL1 using anti-AMSH-LP/STAMBPL1 antibody (A09862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMSH-LP/STAMBPL1 antigen affinity purified polyclonal antibody (Catalog # A09862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMSH-LP/STAMBPL1 at approximately 50 kDa. The expected band size for AMSH-LP/STAMBPL1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09862-1-stambpl1-primary-antibodies-fcm-testing-2.jpgAnti-AMSH-LP/STAMBPL1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-AMSH-LP/STAMBPL1 antibody (A09862-1). Overlay histogram showing 293T cells stained with A09862-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMSH-LP/STAMBPL1 Antibody (A09862-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-swap70-picoband-trade-antibody-a06972-3-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06972-3-swap70-primary-antibodies-wb-testing-1.jpgAnti-SWAP70 Antibody Picoband® Western blot analysis of SWAP70 using anti-SWAP70 antibody (A06972-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SWAP70 antigen affinity purified polyclonal antibody (Catalog # A06972-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SWAP70 at approximately 69 kDa. The expected band size for SWAP70 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06972-3-swap70-primary-antibodies-ihc-testing-2.jpgAnti-SWAP70 Antibody Picoband® IHC analysis of SWAP70 using anti-SWAP70 antibody (A06972-3). SWAP70 was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SWAP70 Antibody (A06972-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06972-3-swap70-primary-antibodies-ihc-testing-3.jpgAnti-SWAP70 Antibody Picoband® IHC analysis of SWAP70 using anti-SWAP70 antibody (A06972-3). SWAP70 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SWAP70 Antibody (A06972-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06972-3-swap70-primary-antibodies-if-testing-4.jpgAnti-SWAP70 Antibody Picoband® IF analysis of SWAP70 using anti-SWAP70 antibody (A06972-3). SWAP70 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SWAP70 Antibody (A06972-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06972-3-swap70-primary-antibodies-if-testing-5.jpgAnti-SWAP70 Antibody Picoband® IF analysis of SWAP70 using anti-SWAP70 antibody (A06972-3). SWAP70 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SWAP70 Antibody (A06972-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06972-3-swap70-primary-antibodies-fcm-testing-6.jpgAnti-SWAP70 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SWAP70 antibody (A06972-3). Overlay histogram showing Jurkat cells stained with A06972-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SWAP70 Antibody (A06972-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-synaptogyrin-3-syngr3-picoband-trade-antibody-a14692-2-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14692-2-syngr3-primary-antibodies-wb-testing-1.jpgAnti-Synaptogyrin 3/SYNGR3 Antibody Picoband® Western blot analysis of Synaptogyrin 3/SYNGR3 using anti-Synaptogyrin 3/SYNGR3 antibody (A14692-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptogyrin 3/SYNGR3 antigen affinity purified polyclonal antibody (Catalog # A14692-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptogyrin 3/SYNGR3 at approximately 24 kDa. The expected band size for Synaptogyrin 3/SYNGR3 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14692-2-syngr3-primary-antibodies-ihc-testing-4.jpgAnti-Synaptogyrin 3/SYNGR3 Antibody Picoband®IHC analysis of Synaptogyrin-3/SYNGR3 using anti-Synaptogyrin-3/SYNGR3 antibody (A14692-2). Synaptogyrin-3/SYNGR3 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptogyrin-3/SYNGR3 Antibody (A14692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14692-2-syngr3-primary-antibodies-ihc-testing-2.jpgAnti-Synaptogyrin 3/SYNGR3 Antibody Picoband® IHC analysis of Synaptogyrin 3/SYNGR3 using anti-Synaptogyrin 3/SYNGR3 antibody (A14692-2). Synaptogyrin 3/SYNGR3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptogyrin 3/SYNGR3 Antibody (A14692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14692-2-syngr3-primary-antibodies-ihc-testing-3.jpgAnti-Synaptogyrin 3/SYNGR3 Antibody Picoband® IHC analysis of Synaptogyrin 3/SYNGR3 using anti-Synaptogyrin 3/SYNGR3 antibody (A14692-2). Synaptogyrin 3/SYNGR3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptogyrin 3/SYNGR3 Antibody (A14692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-wdr33-picoband-trade-antibody-a09589-1-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09589-1-wdr33-primary-antibodies-wb-testing-1.jpgAnti-WDR33 Antibody Picoband® Western blot analysis of WDR33 using anti-WDR33 antibody (A09589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR33 antigen affinity purified polyclonal antibody (Catalog # A09589-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR33 at approximately 170-180 kDa. The expected band size for WDR33 is at 146 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09589-1-wdr33-primary-antibodies-fcm-testing-2.jpgAnti-WDR33 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-WDR33 antibody (A09589-1). Overlay histogram showing 293T cells stained with A09589-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR33 Antibody (A09589-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rab4-rab4a-picoband-trade-antibody-a04643-2-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-wb-testing-1.jpgAnti-Rab4/RAB4A Antibody Picoband® Western blot analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab4/RAB4A antigen affinity purified polyclonal antibody (Catalog # A04643-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab4/RAB4A at approximately 24 kDa. The expected band size for Rab4/RAB4A is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-ihc-testing-2.jpgAnti-Rab4/RAB4A Antibody Picoband® IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Rab4/RAB4A was detected in a paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rab4/RAB4A Antibody (A04643-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-ihc-testing-3.jpgAnti-Rab4/RAB4A Antibody Picoband® IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Rab4/RAB4A was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rab4/RAB4A Antibody (A04643-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-ihc-testing-4.jpgAnti-Rab4/RAB4A Antibody Picoband® IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Rab4/RAB4A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rab4/RAB4A Antibody (A04643-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-ihc-testing-5.jpgAnti-Rab4/RAB4A Antibody Picoband® IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Rab4/RAB4A was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rab4/RAB4A Antibody (A04643-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-ihc-testing-6.jpgAnti-Rab4/RAB4A Antibody Picoband® IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Rab4/RAB4A was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rab4/RAB4A Antibody (A04643-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-ihc-testing-7.jpgAnti-Rab4/RAB4A Antibody Picoband® IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Rab4/RAB4A was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rab4/RAB4A Antibody (A04643-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04643-2-rab4a-primary-antibodies-ihc-testing-8.jpgAnti-Rab4/RAB4A Antibody Picoband® IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (A04643-2). Rab4/RAB4A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rab4/RAB4A Antibody (A04643-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-rab7-rab7a-picoband-trade-antibody-a02409-1-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-wb-testing-1.jpgAnti-RAB7/RAB7A Antibody Picoband® Western blot analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HEL whole cell lysates. Lane 4: rat L6 whole cell lysates. Lane 5: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7/RAB7A antigen affinity purified polyclonal antibody (Catalog # A02409-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB7/RAB7A at approximately 23 kDa. The expected band size for RAB7/RAB7A is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-2.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-3.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-4.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-5.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-6.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-7.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-8.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-9.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-10.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-11.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-12.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-13.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-14.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-15.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-16.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-17.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ihc-testing-18.jpgAnti-RAB7/RAB7A Antibody Picoband® IHC analysis of RAB7/RAB7A using anti-RAB7/RAB7A antibody (A02409-1). RAB7/RAB7A was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB7/RAB7A Antibody (A02409-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-fcm-testing-19.jpgAnti-RAB7/RAB7A Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-RAB7/RAB7A antibody (A02409-1). Overlay histogram showing Jurkat cells stained with A02409-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB7/RAB7A Antibody (A02409-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-fcm-testing-20.jpgAnti-RAB7/RAB7A Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-RAB7/RAB7A antibody (A02409-1). Overlay histogram showing HEPA1-6 cells stained with A02409-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB7/RAB7A Antibody (A02409-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-ip-testing-1.jpgAnti-RAB7/RAB7A Antibody Picoband®Immunoprecipitating RAB7A in A431 whole cell lysate. Western blot analysis of RAB7A using anti-RAB7A antibody (A02409-1). Lane 1: A431 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-RAB7A antibody in A431 whole cell lysate, Lane 3: anti-RAB7A antibody (2μg) + A431 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB7A antigen affinity purified polyclonal antibody (A02409-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB7A at approximately 23 kDa. The expected band size for RAB7A is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02409-1-rab7a-primary-antibodies-fcm-testing-21.jpgAnti-RAB7/RAB7A Antibody Picoband® Flow Cytometry analysis of RH35 cells using anti-RAB7/RAB7A antibody (A02409-1). Overlay histogram showing RH35 cells stained with A02409-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB7/RAB7A Antibody (A02409-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cytokeratin-1-krt1-picoband-trade-antibody-a01639-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01639-krt1-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 1/KRT1 Antibody Picoband® Western blot analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 1/KRT1 antigen affinity purified polyclonal antibody (Catalog # A01639) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 1/KRT1 at approximately 66 kDa. The expected band size for Cytokeratin 1/KRT1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01639-krt1-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 1/KRT1 Antibody Picoband® IHC analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639). Cytokeratin 1/KRT1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01639-krt1-primary-antibodies-if-testing-3.jpgAnti-Cytokeratin 1/KRT1 Antibody Picoband® IF analysis of Cytokeratin 1/KRT1 using anti-Cytokeratin 1/KRT1 antibody (A01639). Cytokeratin 1/KRT1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01639-krt1-primary-antibodies-fcm-testing-4.jpgAnti-Cytokeratin 1/KRT1 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-Cytokeratin 1/KRT1 antibody (A01639). Overlay histogram showing Jurkat cells stained with A01639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 1/KRT1 Antibody (A01639, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cytokeratin-2e-krt2-picoband-trade-antibody-a05667-2-boster.html2026-03-17T05:14:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05667-2-krt2-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 2e/KRT2 Antibody Picoband® Western blot analysis of Cytokeratin 2e/KRT2 using anti-Cytokeratin 2e/KRT2 antibody (A05667-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 2e/KRT2 antigen affinity purified polyclonal antibody (Catalog # A05667-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 2e/KRT2 at approximately 65 kDa. The expected band size for Cytokeratin 2e/KRT2 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05667-2-krt2-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 2e/KRT2 Antibody Picoband® IHC analysis of Cytokeratin 2e/KRT2 using anti-Cytokeratin 2e/KRT2 antibody (A05667-2). Cytokeratin 2e/KRT2 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytokeratin 2e/KRT2 Antibody (A05667-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05667-2-krt2-primary-antibodies-if-testing-3.jpgAnti-Cytokeratin 2e/KRT2 Antibody Picoband® IF analysis of Cytokeratin 2e/KRT2 using anti-Cytokeratin 2e/KRT2 antibody (A05667-2). Cytokeratin 2e/KRT2 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin 2e/KRT2 Antibody (A05667-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05667-2-krt2-primary-antibodies-fcm-testing-4.jpgAnti-Cytokeratin 2e/KRT2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-Cytokeratin 2e/KRT2 antibody (A05667-2). Overlay histogram showing Jurkat cells stained with A05667-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 2e/KRT2 Antibody (A05667-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05667-2-krt2-primary-antibodies-fcm-testing-5.jpgAnti-Cytokeratin 2e/KRT2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Cytokeratin 2e/KRT2 antibody (A05667-2). Overlay histogram showing U251 cells stained with A05667-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 2e/KRT2 Antibody (A05667-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cytokeratin-4-krt4-picoband-trade-antibody-a07410-3-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07410-3-krt4-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 4/KRT4 Antibody Picoband® Western blot analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 4/KRT4 antigen affinity purified polyclonal antibody (Catalog # A07410-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 4/KRT4 at approximately 56 kDa. The expected band size for Cytokeratin 4/KRT4 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07410-3-krt4-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 4/KRT4 Antibody Picoband® IHC analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Cytokeratin 4/KRT4 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytokeratin 4/KRT4 Antibody (A07410-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07410-3-krt4-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 4/KRT4 Antibody Picoband® IHC analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Cytokeratin 4/KRT4 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytokeratin 4/KRT4 Antibody (A07410-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07410-3-krt4-primary-antibodies-ihc-testing-4.jpgAnti-Cytokeratin 4/KRT4 Antibody Picoband® IHC analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Cytokeratin 4/KRT4 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytokeratin 4/KRT4 Antibody (A07410-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07410-3-krt4-primary-antibodies-if-testing-5.jpgAnti-Cytokeratin 4/KRT4 Antibody Picoband® IF analysis of Cytokeratin 4/KRT4 using anti-Cytokeratin 4/KRT4 antibody (A07410-3). Cytokeratin 4/KRT4 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin 4/KRT4 Antibody (A07410-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-suclg1-picoband-trade-antibody-a06274-1-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-wb-testing-1.jpgAnti-SUCLG1 Antibody Picoband® Western blot analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG1 antigen affinity purified polyclonal antibody (Catalog # A06274-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUCLG1 at approximately 35 kDa. The expected band size for SUCLG1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-ihc-testing-2.jpgAnti-SUCLG1 Antibody Picoband® IHC analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). SUCLG1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG1 Antibody (A06274-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-ihc-testing-3.jpgAnti-SUCLG1 Antibody Picoband® IHC analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). SUCLG1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG1 Antibody (A06274-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-ihc-testing-4.jpgAnti-SUCLG1 Antibody Picoband® IHC analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). SUCLG1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG1 Antibody (A06274-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-ihc-testing-5.jpgAnti-SUCLG1 Antibody Picoband® IHC analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). SUCLG1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG1 Antibody (A06274-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-if-testing-6.jpgAnti-SUCLG1 Antibody Picoband® IF analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). SUCLG1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUCLG1 Antibody (A06274-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-if-testing-7.jpgAnti-SUCLG1 Antibody Picoband® IF analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). SUCLG1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SUCLG1 Antibody (A06274-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06274-1-suclg1-primary-antibodies-fcm-testing-8.jpgAnti-SUCLG1 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SUCLG1 antibody (A06274-1). Overlay histogram showing Jurkat cells stained with A06274-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLG1 Antibody (A06274-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-suclg2-picoband-trade-antibody-a08268-1-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-wb-testing-1.jpgAnti-SUCLG2 Antibody Picoband® Western blot analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human COLO320 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG2 antigen affinity purified polyclonal antibody (Catalog # A08268-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUCLG2 at approximately 47 kDa. The expected band size for SUCLG2 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-2.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-3.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-4.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-5.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human prostate adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-6.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-7.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-8.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-9.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-10.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ihc-testing-11.jpgAnti-SUCLG2 Antibody Picoband® IHC analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-if-testing-12.jpgAnti-SUCLG2 Antibody Picoband® IF analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-if-testing-13.jpgAnti-SUCLG2 Antibody Picoband® IF analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). SUCLG2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SUCLG2 Antibody (A08268-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-ip-testing-1.jpgAnti-SUCLG2 Antibody Picoband®Immunoprecipitating (IP) SUCLG2 in K562 whole cell lysate. Western blot analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1); Lane 1: K562 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SUCLG2 antibody in K562 whole cell lysate; Lane 3: anti-SUCLG2 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SUCLG2 antigen affinity purified polyclonal antibody (A08268-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SUCLG2 at approximately 47 kDa. The expected band size for SUCLG2 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08268-1-suclg2-primary-antibodies-fcm-testing-14_1.jpgAnti-SUCLG2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SUCLG2 antibody (A08268-1). Overlay histogram showing Jurkat cells stained with A08268-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLG2 Antibody (A08268-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sugt1-picoband-trade-antibody-a05411-1-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05411-1-sugt1-primary-antibodies-wb-testing-1.jpgAnti-SUGT1 Antibody Picoband® Western blot analysis of SUGT1 using anti-SUGT1 antibody (A05411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUGT1 antigen affinity purified polyclonal antibody (Catalog # A05411-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUGT1 at approximately 36 kDa. The expected band size for SUGT1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05411-1-sugt1-primary-antibodies-if-testing-2.jpgAnti-SUGT1 Antibody Picoband® IF analysis of SUGT1 using anti-SUGT1 antibody (A05411-1) and anti-Tubulin Alpha antibody (M03989-3). SUGT1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUGT1 Antibody (A05411-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05411-1-sugt1-primary-antibodies-fcm-testing-3.jpgAnti-SUGT1 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-SUGT1 antibody (A05411-1). Overlay histogram showing U251 cells stained with A05411-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUGT1 Antibody (A05411-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sumf2-picoband-trade-antibody-a08890-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-wb-testing-1.jpgAnti-SUMF2 Antibody Picoband® Western blot analysis of SUMF2 using anti-SUMF2 antibody (A08890). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUMF2 antigen affinity purified polyclonal antibody (Catalog # A08890) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUMF2 at approximately 36 kDa. The expected band size for SUMF2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-ihc-testing-2.jpgAnti-SUMF2 Antibody Picoband® IHC analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-ihc-testing-3.jpgAnti-SUMF2 Antibody Picoband® IHC analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-ihc-testing-4.jpgAnti-SUMF2 Antibody Picoband® IHC analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human ovarian tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-ihc-testing-5.jpgAnti-SUMF2 Antibody Picoband® IHC analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-ihc-testing-6.jpgAnti-SUMF2 Antibody Picoband® IHC analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-if-testing-7.jpgAnti-SUMF2 Antibody Picoband® IF analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-if-testing-8.jpgAnti-SUMF2 Antibody Picoband® IF analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-if-testing-9.jpgAnti-SUMF2 Antibody Picoband® IF analysis of SUMF2 using anti-SUMF2 antibody (A08890). SUMF2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SUMF2 Antibody (A08890) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08890-sumf2-primary-antibodies-fcm-testing-10.jpgAnti-SUMF2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-SUMF2 antibody (A08890). Overlay histogram showing U87 cells stained with A08890 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUMF2 Antibody (A08890, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sulfite-oxidase-suox-picoband-trade-antibody-a05838-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05838-suox-primary-antibodies-wb-testing-1.jpgAnti-Sulfite oxidase/SUOX Antibody Picoband® Western blot analysis of Sulfite oxidase/SUOX using anti-Sulfite oxidase/SUOX antibody (A05838). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sulfite oxidase/SUOX antigen affinity purified polyclonal antibody (Catalog # A05838) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sulfite oxidase/SUOX at approximately 60 kDa. The expected band size for Sulfite oxidase/SUOX is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05838-suox-primary-antibodies-fcm-testing-2.jpgAnti-Sulfite oxidase/SUOX Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Sulfite oxidase/SUOX antibody (A05838). Overlay histogram showing HepG2 cells stained with A05838 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sulfite oxidase/SUOX Antibody (A05838, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-surf1-picoband-trade-antibody-a03678-2-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03678-2-surf1-primary-antibodies-wb-testing-1.jpgAnti-SURF1 Antibody Picoband® Western blot analysis of SURF1 using anti-SURF1 antibody (A03678-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF1 antigen affinity purified polyclonal antibody (Catalog # A03678-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF1 at approximately 33 kDa. The expected band size for SURF1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03678-2-surf1-primary-antibodies-if-testing-2.jpgAnti-SURF1 Antibody Picoband® IF analysis of SURF1 using anti-SURF1 antibody (A03678-2). SURF1 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SURF1 Antibody (A03678-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03678-2-surf1-primary-antibodies-fcm-testing-3.jpgAnti-SURF1 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SURF1 antibody (A03678-2). Overlay histogram showing Jurkat cells stained with A03678-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF1 Antibody (A03678-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sa2-stag2-picoband-trade-antibody-a03624-1-boster.html2026-03-17T05:14:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-wb-testing-1.jpgAnti-SA2/STAG2 Antibody Picoband® Western blot analysis of SA2/STAG2 using anti-SA2/STAG2 antibody (A03624-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: huamn RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA2/STAG2 antigen affinity purified polyclonal antibody (Catalog # A03624-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SA2/STAG2 at approximately 141 kDa. The expected band size for SA2/STAG2 is at 141 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-2.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-3.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-4.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-5.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-6.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-7.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-8.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-9.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-ihc-testing-10.jpgAnti-SA2/STAG2 Antibody Picoband® IHC analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-if-testing-11.jpgAnti-SA2/STAG2 Antibody Picoband® IF analysis of STAG2 using anti-STAG2 antibody (A03624-1) and anti-Tubulin Alpha antibody (M03989-3). STAG2 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAG2 Antibody (A03624-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-if-testing-12_1.jpgAnti-SA2/STAG2 Antibody Picoband® IF analysis of STAG2 using anti-STAG2 antibody (A03624-1). STAG2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-STAG2 Antibody (A03624-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-fcm-testing-13.jpgAnti-SA2/STAG2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-STAG2 antibody (A03624-1). Overlay histogram showing U937 cells stained with A03624-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAG2 Antibody (A03624-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03624-1-stag2-primary-antibodies-fcm-testing-14.jpgAnti-SA2/STAG2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-STAG2 antibody (A03624-1). Overlay histogram showing 293T cells stained with A03624-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAG2 Antibody (A03624-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-amphiphysin-amph-picoband-trade-antibody-a02364-1-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02364-1-amph-primary-antibodies-wb-testing-1.jpgAnti-Amphiphysin/AMPH Antibody Picoband® Western blot analysis of Amphiphysin/AMPH using anti-Amphiphysin/AMPH antibody (A02364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiphysin/AMPH antigen affinity purified polyclonal antibody (Catalog # A02364-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiphysin/AMPH at approximately 115-125 kDa. The expected band size for Amphiphysin/AMPH is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02364-1-amph-primary-antibodies-fcm-testing-2.jpgAnti-Amphiphysin/AMPH Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Amphiphysin/AMPH antibody (A02364-1). Overlay histogram showing U87 cells stained with A02364-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Amphiphysin/AMPH Antibody (A02364-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sarcalumenin-srl-picoband-trade-antibody-a07699-4-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07699-4-srl-primary-antibodies-wb-testing-1.jpgAnti-Sarcalumenin/Srl Antibody Picoband® Western blot analysis of Sarcalumenin/Srl using anti-Sarcalumenin/Srl antibody (A07699-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sarcalumenin/Srl antigen affinity purified polyclonal antibody (Catalog # A07699-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sarcalumenin/Srl at approximately 54 kDa. The expected band size for Sarcalumenin/Srl is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07699-4-srl-primary-antibodies-ihc-testing-2.jpgAnti-Sarcalumenin/Srl Antibody Picoband® IHC analysis of Sarcalumenin/Srl using anti-Sarcalumenin/Srl antibody (A07699-4). Sarcalumenin/Srl was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sarcalumenin/Srl Antibody (A07699-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07699-4-srl-primary-antibodies-fcm-testing-3.jpgAnti-Sarcalumenin/Srl Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Sarcalumenin/Srl antibody (A07699-4). Overlay histogram showing HEPA1-6 cells stained with A07699-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Sarcalumenin/Srl Antibody (A07699-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spermidine-synthase-srm-picoband-trade-antibody-a01594-2-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01594-2-srm-primary-antibodies-wb-testing-1.jpgAnti-Spermidine synthase/SRM Antibody Picoband® Western blot analysis of Spermidine synthase/SRM using anti-Spermidine synthase/SRM antibody (A01594-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spermidine synthase/SRM antigen affinity purified polyclonal antibody (Catalog # A01594-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spermidine synthase/SRM at approximately 34 kDa. The expected band size for Spermidine synthase/SRM is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01594-2-srm-primary-antibodies-fcm-testing-2.jpgAnti-Spermidine synthase/SRM Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Spermidine synthase/SRM antibody (A01594-2). Overlay histogram showing HepG2 cells stained with A01594-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Spermidine synthase/SRM Antibody (A01594-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ston1-picoband-trade-antibody-a13140-2-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13140-2-ston1-primary-antibodies-wb-testing-1.jpgAnti-STON1 Antibody Picoband® Western blot analysis of STON1 using anti-STON1 antibody (A13140-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STON1 antigen affinity purified polyclonal antibody (Catalog # A13140-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STON1 at approximately 83 kDa. The expected band size for STON1 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13140-2-ston1-primary-antibodies-ihc-testing-2.jpgAnti-STON1 Antibody Picoband® IHC analysis of STON1 using anti-STON1 antibody (A13140-2). STON1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON1 Antibody (A13140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13140-2-ston1-primary-antibodies-ihc-testing-3.jpgAnti-STON1 Antibody Picoband® IHC analysis of STON1 using anti-STON1 antibody (A13140-2). STON1 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON1 Antibody (A13140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13140-2-ston1-primary-antibodies-ihc-testing-4.jpgAnti-STON1 Antibody Picoband® IHC analysis of STON1 using anti-STON1 antibody (A13140-2). STON1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON1 Antibody (A13140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13140-2-ston1-primary-antibodies-ihc-testing-5.jpgAnti-STON1 Antibody Picoband® IHC analysis of STON1 using anti-STON1 antibody (A13140-2). STON1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STON1 Antibody (A13140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13140-2-ston1-primary-antibodies-if-testing-6.jpgAnti-STON1 Antibody Picoband® IF analysis of STON1 using anti-STON1 antibody (A13140-2). STON1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-STON1 Antibody (A13140-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13140-2-ston1-primary-antibodies-fcm-testing-7.jpgAnti-STON1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-STON1 antibody (A13140-2). Overlay histogram showing 293T cells stained with A13140-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STON1 Antibody (A13140-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-stpg2-picoband-trade-antibody-a19412-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a19412-stpg2-primary-antibodies-wb-testing-1.jpgAnti-STPG2 Antibody Picoband® Western blot analysis of STPG2 using anti-STPG2 antibody (A19412). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STPG2 antigen affinity purified polyclonal antibody (Catalog # A19412) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STPG2 at approximately 51 kDa. The expected band size for STPG2 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a19412-stpg2-primary-antibodies-fcm-testing-2.jpgAnti-STPG2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-STPG2 antibody (A19412). Overlay histogram showing 293T cells stained with A19412 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STPG2 Antibody (A19412, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-syntaxin-18-stx18-picoband-trade-antibody-a11895-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-wb-testing-1.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® Western blot analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 18/STX18 antigen affinity purified polyclonal antibody (Catalog # A11895) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 18/STX18 at approximately 39 kDa. The expected band size for Syntaxin 18/STX18 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-ihc-testing-2.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Syntaxin 18/STX18 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaxin 18/STX18 Antibody (A11895) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-ihc-testing-3.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Syntaxin 18/STX18 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaxin 18/STX18 Antibody (A11895) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-ihc-testing-4.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Syntaxin 18/STX18 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaxin 18/STX18 Antibody (A11895) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-ihc-testing-5.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Syntaxin 18/STX18 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaxin 18/STX18 Antibody (A11895) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-ihc-testing-6.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® IHC analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Syntaxin 18/STX18 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaxin 18/STX18 Antibody (A11895) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-if-testing-7.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® IF analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Syntaxin 18/STX18 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Syntaxin 18/STX18 Antibody (A11895) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-if-testing-8.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® IF analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Syntaxin 18/STX18 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Syntaxin 18/STX18 Antibody (A11895) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11895-stx18-primary-antibodies-fcm-testing-9.jpgAnti-Syntaxin 18/STX18 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-Syntaxin 18/STX18 antibody (A11895). Overlay histogram showing U937 cells stained with A11895 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 18/STX18 Antibody (A11895, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-synip-stxbp4-picoband-trade-antibody-a08343-1-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08343-1-stxbp4-primary-antibodies-wb-testing-1_1.jpgAnti-Synip/STXBP4 Antibody Picoband®Western blot analysis of Synip/STXBP4 using anti-Synip/STXBP4 antibody (A08343-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synip/STXBP4 antigen affinity purified polyclonal antibody (Catalog # A08343-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Synip/STXBP4 at approximately 68 kDa. The expected band size for Synip/STXBP4 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08343-1-stxbp4-primary-antibodies-ihc-testing-1.jpgAnti-Synip/STXBP4 Antibody Picoband®IHC analysis of Synip/STXBP4 using anti-Synip/STXBP4 antibody (A08343-1). Synip/STXBP4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synip/STXBP4 Antibody (A08343-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08343-1-stxbp4-primary-antibodies-ihc-testing-2.jpgAnti-Synip/STXBP4 Antibody Picoband®IHC analysis of Synip/STXBP4 using anti-Synip/STXBP4 antibody (A08343-1). Synip/STXBP4 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synip/STXBP4 Antibody (A08343-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08343-1-stxbp4-primary-antibodies-fcm-testing-1.jpgAnti-Synip/STXBP4 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-Synip/STXBP4 antibody (A08343-1). Overlay histogram showing HepG2 cells stained with A08343-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synip/STXBP4 Antibody (A08343-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-surf2-picoband-trade-antibody-a14641-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14641-surf2-primary-antibodies-wb-testing-1.jpgAnti-SURF2 Antibody Picoband® Western blot analysis of SURF2 using anti-SURF2 antibody (A14641). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF2 antigen affinity purified polyclonal antibody (Catalog # A14641) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF2 at approximately 38 kDa. The expected band size for SURF2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14641-surf2-primary-antibodies-if-testing-2.jpgAnti-SURF2 Antibody Picoband® IF analysis of SURF2 using anti-SURF2 antibody (A14641) and anti-Tubulin Alpha antibody (M03989-3). SURF2 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SURF2 Antibody (A14641) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14641-surf2-primary-antibodies-fcm-testing-3.jpgAnti-SURF2 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-SURF2 antibody (A14641). Overlay histogram showing U937 cells stained with A14641 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF2 Antibody (A14641, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14641-surf2-primary-antibodies-fcm-testing-4.jpgAnti-SURF2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SURF2 antibody (A14641). Overlay histogram showing 293T cells stained with A14641 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SURF2 Antibody (A14641, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spt6-supt6h-picoband-trade-antibody-a06484-1-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06484-1-supt6h-primary-antibodies-wb-testing-1_1.jpgAnti-Spt6/SUPT6H Antibody Picoband® Western blot analysis of SUPT6H using anti-SUPT6H antibody (A06484-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUPT6H antigen affinity purified polyclonal antibody (A06484-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUPT6H at approximately 250 kDa. The expected band size for SUPT6H is at 199 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06484-1-supt6h-primary-antibodies-wb-testing-2.jpgAnti-Spt6/SUPT6H Antibody Picoband® Western blot analysis of SUPT6H using anti-SUPT6H antibody (A06484-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUPT6H antigen affinity purified polyclonal antibody (A06484-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUPT6H at approximately 250 kDa. The expected band size for SUPT6H is at 199 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06484-1-supt6h-primary-antibodies-if-testing-3.jpgAnti-Spt6/SUPT6H Antibody Picoband® IF analysis of SUPT6H using anti-SUPT6H antibody (A06484-1). SUPT6H was detected in an immunocytochemical section of TPC1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUPT6H Antibody (A06484-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 594 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06484-1-supt6h-primary-antibodies-fcm-testing-4.jpgAnti-Spt6/SUPT6H Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SUPT6H antibody (A06484-1). Overlay histogram showing SiHa cells stained with A06484-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUPT6H Antibody (A06484-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-susd6-picoband-trade-antibody-a14613-1-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14613-1-susd6-primary-antibodies-wb-testing-1.jpgAnti-SUSD6 Antibody Picoband® Western blot analysis of SUSD6 using anti-SUSD6 antibody (A14613-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUSD6 antigen affinity purified polyclonal antibody (Catalog # A14613-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUSD6 at approximately 35 kDa. The expected band size for SUSD6 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14613-1-susd6-primary-antibodies-fcm-testing-2.jpgAnti-SUSD6 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SUSD6 antibody (A14613-1). Overlay histogram showing Jurkat cells stained with A14613-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUSD6 Antibody (A14613-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-symplekin-sympk-picoband-trade-antibody-a05709-1-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-wb-testing-1.jpgAnti-Symplekin/SYMPK Antibody Picoband® Western blot analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (A05709-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Symplekin/SYMPK antigen affinity purified polyclonal antibody (Catalog # A05709-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Symplekin/SYMPK at approximately 150 kDa. The expected band size for Symplekin/SYMPK is at 141 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-ihc-testing-2.jpgAnti-Symplekin/SYMPK Antibody Picoband® IHC analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (A05709-1). Symplekin/SYMPK was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Symplekin/SYMPK Antibody (A05709-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-ihc-testing-3.jpgAnti-Symplekin/SYMPK Antibody Picoband® IHC analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (A05709-1). Symplekin/SYMPK was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Symplekin/SYMPK Antibody (A05709-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-ihc-testing-4.jpgAnti-Symplekin/SYMPK Antibody Picoband® IHC analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (A05709-1). Symplekin/SYMPK was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Symplekin/SYMPK Antibody (A05709-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-ihc-testing-5.jpgAnti-Symplekin/SYMPK Antibody Picoband® IHC analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (A05709-1). Symplekin/SYMPK was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Symplekin/SYMPK Antibody (A05709-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-ihc-testing-6.jpgAnti-Symplekin/SYMPK Antibody Picoband® IHC analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (A05709-1). Symplekin/SYMPK was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Symplekin/SYMPK Antibody (A05709-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-if-testing-7.jpgAnti-Symplekin/SYMPK Antibody Picoband® IF analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (A05709-1) and anti-Tubulin Alpha antibody (M03989-3). Symplekin/SYMPK was detected in immunocytochemical section of T47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Symplekin/SYMPK Antibody (A05709-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05709-1-sympk-primary-antibodies-fcm-testing-8.jpgAnti-Symplekin/SYMPK Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Symplekin/SYMPK antibody (A05709-1). Overlay histogram showing U87 cells stained with A05709-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Symplekin/SYMPK Antibody (A05709-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nesprin-2-syne2-picoband-trade-antibody-a02818-1-boster.html2026-03-17T05:14:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02818-1-syne2-primary-antibodies-wb-testing-1.jpgAnti-Nesprin 2/SYNE2 Antibody Picoband® Western blot analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody (A02818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin 2/SYNE2 antigen affinity purified polyclonal antibody (Catalog # A02818-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nesprin 2/SYNE2 at approximately 50-60 kDa. The expected band size for Nesprin 2/SYNE2 is at 796 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02818-1-syne2-primary-antibodies-ihc-testing-2.jpgAnti-Nesprin 2/SYNE2 Antibody Picoband® IHC analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody (A02818-1). Nesprin 2/SYNE2 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nesprin 2/SYNE2 Antibody (A02818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02818-1-syne2-primary-antibodies-ihc-testing-3.jpgAnti-Nesprin 2/SYNE2 Antibody Picoband® IHC analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody (A02818-1). Nesprin 2/SYNE2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nesprin 2/SYNE2 Antibody (A02818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02818-1-syne2-primary-antibodies-ihc-testing-4.jpgAnti-Nesprin 2/SYNE2 Antibody Picoband® IHC analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody (A02818-1). Nesprin 2/SYNE2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nesprin 2/SYNE2 Antibody (A02818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02818-1-syne2-primary-antibodies-if-testing-5.jpgAnti-Nesprin 2/SYNE2 Antibody Picoband® IF analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody (A02818-1). Nesprin 2/SYNE2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Nesprin 2/SYNE2 Antibody (A02818-1) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02818-1-syne2-primary-antibodies-fcm-testing-6.jpgAnti-Nesprin 2/SYNE2 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Nesprin 2/SYNE2 antibody (A02818-1). Overlay histogram showing U87 cells stained with A02818-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin 2/SYNE2 Antibody (A02818-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wdr24-picoband-trade-antibody-a12216-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12216-wdr24-primary-antibodies-wb-testing-1.jpgAnti-WDR24 Antibody Picoband® Western blot analysis of WDR24 using anti-WDR24 antibody (A12216). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR24 antigen affinity purified polyclonal antibody (Catalog # A12216) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR24 at approximately 88 kDa. The expected band size for WDR24 is at 88 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-wdr36-picoband-trade-antibody-a05779-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05779-1-wdr36-primary-antibodies-wb-testing-1.jpgAnti-WDR36 Antibody Picoband® Western blot analysis of WDR36 using anti-WDR36 antibody (A05779-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR36 antigen affinity purified polyclonal antibody (Catalog # A05779-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR36 at approximately 105 kDa. The expected band size for WDR36 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05779-1-wdr36-primary-antibodies-if-testing-2.jpgAnti-WDR36 Antibody Picoband® IF analysis of WDR36 using anti-WDR36 antibody (A05779-1) and anti-Tubulin Alpha antibody (M03989-3). WDR36 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WDR36 Antibody (A05779-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05779-1-wdr36-primary-antibodies-fcm-testing-3.jpgAnti-WDR36 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-WDR36 antibody (A05779-1). Overlay histogram showing A431 cells stained with A05779-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR36 Antibody (A05779-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05779-1-wdr36-primary-antibodies-fcm-testing-4.jpgAnti-WDR36 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-WDR36 antibody (A05779-1). Overlay histogram showing U251 cells stained with A05779-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR36 Antibody (A05779-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-syt16-picoband-trade-antibody-a13306-2-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13306-2-syt16-primary-antibodies-wb-testing-1.jpgAnti-SYT16 Antibody Picoband® Western blot analysis of SYT16 using anti-SYT16 antibody (A13306-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYT16 antigen affinity purified polyclonal antibody (Catalog # A13306-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYT16 at approximately 72 kDa. The expected band size for SYT16 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13306-2-syt16-primary-antibodies-if-testing-2.jpgAnti-SYT16 Antibody Picoband® IF analysis of SYT16 using anti-SYT16 antibody (A13306-2). SYT16 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SYT16 Antibody (A13306-2) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13306-2-syt16-primary-antibodies-fcm-testing-3.jpgAnti-SYT16 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-SYT16 antibody (A13306-2). Overlay histogram showing U251 cells stained with A13306-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYT16 Antibody (A13306-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-stmn3-picoband-trade-antibody-a08661-2-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08661-2-stmn3-primary-antibodies-wb-testing-1.jpgAnti-STMN3 Antibody Picoband® Western blot analysis of STMN3 using anti-STMN3 antibody (A08661-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STMN3 antigen affinity purified polyclonal antibody (Catalog # A08661-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STMN3 at approximately 21-25 kDa. The expected band size for STMN3 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08661-2-stmn3-primary-antibodies-fcm-testing-2.jpgAnti-STMN3 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-STMN3 antibody (A08661-2). Overlay histogram showing THP-1 cells stained with A08661-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STMN3 Antibody (A08661-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-serine-racemase-srr-picoband-trade-antibody-a02660-2-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02660-2-srr-primary-antibodies-wb-testing-1.jpgAnti-Serine racemase/SRR Antibody Picoband® Western blot analysis of Serine racemase/SRR using anti-Serine racemase/SRR antibody (A02660-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serine racemase/SRR antigen affinity purified polyclonal antibody (Catalog # A02660-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Serine racemase/SRR at approximately 37 kDa. The expected band size for Serine racemase/SRR is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02660-2-srr-primary-antibodies-ihc-testing-2.jpgAnti-Serine racemase/SRR Antibody Picoband® IHC analysis of Serine racemase/SRR using anti-Serine racemase/SRR antibody (A02660-2). Serine racemase/SRR was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Serine racemase/SRR Antibody (A02660-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02660-2-srr-primary-antibodies-if-testing-3.jpgAnti-Serine racemase/SRR Antibody Picoband® IF analysis of Serine racemase/SRR using anti-Serine racemase/SRR antibody (A02660-2). Serine racemase/SRR was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Serine racemase/SRR Antibody (A02660-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02660-2-srr-primary-antibodies-ip-testing-4.jpgAnti-Serine racemase/SRR Antibody Picoband® Immunoprecipitating Serine racemase/SRR in SH-SY5Y whole cell lysate. Western blot analysis of Serine racemase/SRR using anti-Serine racemase/SRR antibody (A02660-2). Lane 1: SH-SY5Y whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-Serine racemase/SRR antibody in SH-SY5Y whole cell lysate; Lane 3: anti-Serine racemase/SRR antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Serine racemase/SRR antigen affinity purified polyclonal antibody (A02660-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Serine racemase/SRR at approximately 37 kDa. The expected band size for Serine racemase/SRR is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02660-2-srr-primary-antibodies-fcm-testing-5.jpgAnti-Serine racemase/SRR Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-Serine racemase/SRR antibody (A02660-2). Overlay histogram showing Jurkat cells stained with A02660-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Serine racemase/SRR Antibody (A02660-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ars2-srrt-picoband-trade-antibody-a05444-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-wb-testing-1_1.jpgAnti-ARS2/SRRT Antibody Picoband® Western blot analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARS2/SRRT antigen affinity purified polyclonal antibody (A05444-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARS2/SRRT at approximately 101 kDa. The expected band size for ARS2/SRRT is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-2_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-3_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-4_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-5_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-6_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-7_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-8_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-9_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-10_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-11_1.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-12.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-13.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ihc-testing-14.jpgAnti-ARS2/SRRT Antibody Picoband® IHC analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1). ARS2/SRRT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARS2/SRRT Antibody (A05444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-ip-testing-15.jpgAnti-ARS2/SRRT Antibody Picoband® Immunoprecipitating SRRT in MCF-7 whole cell lysate. Western blot analysis of SRRT using anti-SRRT antibody (A05444-1). Lane 1: MCF-7 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SRRT antibody in MCF-7 whole cell lysate; Lane 3: anti-SRRT antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SRRT antigen affinity purified polyclonal antibody (A05444-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SRRT at approximately 101 kDa. The expected band size for SRRT is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-if-testing-16.jpgAnti-ARS2/SRRT Antibody Picoband® IF analysis of ARS2/SRRT using anti-ARS2/SRRT antibody (A05444-1) and anti-Tubulin Alpha antibody (M03989-3). ARS2/SRRT was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ARS2/SRRT Antibody (A05444-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Catalog # BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (Catalog # BA1032) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05444-1-srrt-primary-antibodies-fcm-testing-17.jpgAnti-ARS2/SRRT Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-ARS2/SRRT antibody (A05444-1). Overlay histogram showing MCF-7 cells stained with A05444-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARS2/SRRT Antibody (A05444-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ssrp1-picoband-trade-antibody-a02606-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-wb-testing-1.jpgAnti-SSRP1 Antibody Picoband® Western blot analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human DLD-1 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysate, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSRP1 antigen affinity purified polyclonal antibody (Catalog # A02606-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSRP1 at approximately 92 kDa. The expected band size for SSRP1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-ihc-testing-2.jpgAnti-SSRP1 Antibody Picoband® IHC analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). SSRP1 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSRP1 Antibody (A02606-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-ihc-testing-3.jpgAnti-SSRP1 Antibody Picoband® IHC analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). SSRP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSRP1 Antibody (A02606-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-ihc-testing-4.jpgAnti-SSRP1 Antibody Picoband® IHC analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). SSRP1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSRP1 Antibody (A02606-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-ihc-testing-5.jpgAnti-SSRP1 Antibody Picoband® IHC analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). SSRP1 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSRP1 Antibody (A02606-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-ihc-testing-6.jpgAnti-SSRP1 Antibody Picoband® IHC analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). SSRP1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSRP1 Antibody (A02606-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-if-testing-7.jpgAnti-SSRP1 Antibody Picoband® IF analysis of SSRP1 using anti-SSRP1 antibody (A02606-1) and anti-Tubulin Alpha antibody (M03989-3). SSRP1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SSRP1 Antibody (A02606-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02606-1-ssrp1-primary-antibodies-fcm-testing-8.jpgAnti-SSRP1 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-SSRP1 antibody (A02606-1). Overlay histogram showing U251 cells stained with A02606-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSRP1 Antibody (A02606-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cdw75-st6gal1-picoband-trade-antibody-a03156-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03156-1-st6gal1-primary-antibodies-wb-testing-1.jpgAnti-CDw75/ST6GAL1 Antibody Picoband® Western blot analysis of CDw75/ST6GAL1 using anti-CDw75/ST6GAL1 antibody (A03156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDw75/ST6GAL1 antigen affinity purified polyclonal antibody (Catalog # A03156-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDw75/ST6GAL1 at approximately 47 kDa. The expected band size for CDw75/ST6GAL1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03156-1-st6gal1-primary-antibodies-fcm-testing-2.jpgAnti-CDw75/ST6GAL1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CDw75/ST6GAL1 antibody (A03156-1). Overlay histogram showing HepG2 cells stained with A03156-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDw75/ST6GAL1 Antibody (A03156-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-stap-1-stap1-picoband-trade-antibody-a09142-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09142-1-stap1-primary-antibodies-wb-testing-1.jpgAnti-STAP-1/STAP1 Antibody Picoband® Western blot analysis of STAP-1/STAP1 using anti-STAP-1/STAP1 antibody (A09142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAP-1/STAP1 antigen affinity purified polyclonal antibody (Catalog # A09142-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAP-1/STAP1 at approximately 36 kDa. The expected band size for STAP-1/STAP1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09142-1-stap1-primary-antibodies-fcm-testing-2.jpgAnti-STAP-1/STAP1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-STAP-1/STAP1 antibody (A09142-1). Overlay histogram showing U937 cells stained with A09142-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAP-1/STAP1 Antibody (A09142-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-stap2-picoband-trade-antibody-a08413-3-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08413-3-stap2-primary-antibodies-wb-testing-1.jpgAnti-STAP2 Antibody Picoband® Western blot analysis of STAP2 using anti-STAP2 antibody (A08413-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAP2 antigen affinity purified polyclonal antibody (Catalog # A08413-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAP2 at approximately 50-55 kDa. The expected band size for STAP2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08413-3-stap2-primary-antibodies-ihc-testing-2.jpgAnti-STAP2 Antibody Picoband® IHC analysis of STAP2 using anti-STAP2 antibody (A08413-3). STAP2 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAP2 Antibody (A08413-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08413-3-stap2-primary-antibodies-ihc-testing-3.jpgAnti-STAP2 Antibody Picoband® IHC analysis of STAP2 using anti-STAP2 antibody (A08413-3). STAP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAP2 Antibody (A08413-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08413-3-stap2-primary-antibodies-fcm-testing-4.jpgAnti-STAP2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-STAP2 antibody (A08413-3). Overlay histogram showing A431 cells stained with A08413-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAP2 Antibody (A08413-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mln64-stard3-picoband-trade-antibody-a05385-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05385-1-stard3-primary-antibodies-wb-testing-1.jpgAnti-MLN64/STARD3 Antibody Picoband® Western blot analysis of MLN64/STARD3 using anti-MLN64/STARD3 antibody (A05385-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLN64/STARD3 antigen affinity purified polyclonal antibody (Catalog # A05385-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLN64/STARD3 at approximately 51 kDa. The expected band size for MLN64/STARD3 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-hrd1-syvn1-picoband-trade-antibody-a02670-3-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02670-3-syvn1-primary-antibodies-wb-testing-1.jpgAnti-HRD1/SYVN1 Antibody Picoband® Western blot analysis of HRD1/SYVN1 using anti-HRD1/SYVN1 antibody (A02670-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pamcreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRD1/SYVN1 antigen affinity purified polyclonal antibody (Catalog # A02670-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRD1/SYVN1 at approximately 76 kDa. The expected band size for HRD1/SYVN1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02670-3-syvn1-primary-antibodies-if-testing-2.jpgAnti-HRD1/SYVN1 Antibody Picoband® IF analysis of HRD1/SYVN1 using anti-HRD1/SYVN1 antibody (A02670-3). HRD1/SYVN1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HRD1/SYVN1 Antibody (A02670-3) overnight at 4°C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02670-3-syvn1-primary-antibodies-ip-testing-1.jpgAnti-HRD1/SYVN1 Antibody Picoband®Immunoprecipitating (IP) HRD1/SYVN1 in HepG2 whole cell lysate. Western blot analysis of HRD1/SYVN1 using anti-HRD1/SYVN1 antibody (A02670-3); Lane 1: HepG2 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-HRD1/SYVN1 antibody in HepG2 whole cell lysate; Lane 3: anti-HRD1/SYVN1 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HRD1/SYVN1 antigen affinity purified polyclonal antibody (A02670-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for HRD1/SYVN1 at approximately 76 kDa. The expected band size for HRD1/SYVN1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02670-3-syvn1-primary-antibodies-fcm-testing-3.jpgAnti-HRD1/SYVN1 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-HRD1/SYVN1 antibody (A02670-3). Overlay histogram showing U251 cells stained with A02670-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HRD1/SYVN1 Antibody (A02670-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-qars1-picoband-trade-antibody-a06953-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-wb-testing-1.jpgAnti-QARS1 Antibody Picoband® Western blot analysis of QARS1 using anti-QARS1 antibody (A06953-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QARS1 antigen affinity purified polyclonal antibody (Catalog # A06953-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for QARS1 at approximately 88 kDa. The expected band size for QARS1 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-ihc-testing-2.jpgAnti-QARS1 Antibody Picoband® IHC analysis of QARS1 using anti-QARS1 antibody (A06953-1). QARS1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-QARS1 Antibody (A06953-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-ihc-testing-3.jpgAnti-QARS1 Antibody Picoband® IHC analysis of QARS1 using anti-QARS1 antibody (A06953-1). QARS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-QARS1 Antibody (A06953-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-ihc-testing-4.jpgAnti-QARS1 Antibody Picoband® IHC analysis of QARS1 using anti-QARS1 antibody (A06953-1). QARS1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-QARS1 Antibody (A06953-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-ihc-testing-5.jpgAnti-QARS1 Antibody Picoband® IHC analysis of QARS1 using anti-QARS1 antibody (A06953-1). QARS1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-QARS1 Antibody (A06953-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-if-testing-6.jpgAnti-QARS1 Antibody Picoband® IF analysis of QARS1 using anti-QARS1 antibody (A06953-1). QARS1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-QARS1 Antibody (A06953-1) overnight at 4°C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-if-testing-7.jpgAnti-QARS1 Antibody Picoband® IF analysis of QARS1 using anti-QARS1 antibody (A06953-1). QARS1 was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-QARS1 Antibody (A06953-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-if-testing-8.jpgAnti-QARS1 Antibody Picoband® IF analysis of QARS1 using anti-QARS1 antibody (A06953-1). QARS1 was detected in a paraffin-embedded section of human thyroid carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-QARS1 Antibody (A06953-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06953-1-qars1-primary-antibodies-fcm-testing-9.jpgAnti-QARS1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-QARS1 antibody (A06953-1). Overlay histogram showing THP-1 cells stained with A06953-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QARS1 Antibody (A06953-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-acy-1-acy1-picoband-trade-antibody-a05650-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05650-1-acy1-primary-antibodies-wb-testing-1.jpgAnti-ACY-1/ACY1 Antibody Picoband® Western blot analysis of ACY-1/ACY1 using anti-ACY-1/ACY1 antibody (A05650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brian tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACY-1/ACY1 antigen affinity purified polyclonal antibody (Catalog # A05650-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACY-1/ACY1 at approximately 40-43 kDa. The expected band size for ACY-1/ACY1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05650-1-acy1-primary-antibodies-fcm-testing-2.jpgAnti-ACY-1/ACY1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-ACY-1/ACY1 antibody (A05650-1). Overlay histogram showing THP-1 cells stained with A05650-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACY-1/ACY1 Antibody (A05650-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-t12-cd6-picoband-trade-antibody-a03913-3-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03913-3-cd6-primary-antibodies-wb-testing-1.jpgAnti-T12/CD6 Antibody Picoband® Western blot analysis of T12/CD6 using anti-T12/CD6 antibody (A03913-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-T12/CD6 antigen affinity purified polyclonal antibody (Catalog # A03913-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for T12/CD6 at approximately 135 kDa. The expected band size for T12/CD6 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03913-3-cd6-primary-antibodies-fcm-testing-2.jpgAnti-T12/CD6 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-T12/CD6 antibody (A03913-3). Overlay histogram showing Jurkat cells stained with A03913-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-T12/CD6 Antibody (A03913-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-wdr13-picoband-trade-antibody-a13007-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13007-1-wdr13-primary-antibodies-wb-testing-1.jpgAnti-WDR13 Antibody Picoband® Western blot analysis of WDR13 using anti-WDR13 antibody (A13007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR13 antigen affinity purified polyclonal antibody (Catalog # A13007-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR13 at approximately 56 kDa. The expected band size for WDR13 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13007-1-wdr13-primary-antibodies-fcm-testing-2.jpgAnti-WDR13 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-WDR13 antibody (A13007-1). Overlay histogram showing HepG2 cells stained with A13007-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR13 Antibody (A13007-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-slp2-sytl2-picoband-trade-antibody-a08255-1-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08255-1-sytl2-primary-antibodies-wb-testing-1.jpgAnti-SLP2/SYTL2 Antibody Picoband® Western blot analysis of SLP2/SYTL2 using anti-SLP2/SYTL2 antibody (A08255-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLP2/SYTL2 antigen affinity purified polyclonal antibody (Catalog # A08255-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLP2/SYTL2 at approximately 120-130 kDa. The expected band size for SLP2/SYTL2 is at 105 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-qprt-picoband-trade-antibody-a09896-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09896-qprt-primary-antibodies-wb-testing-1.jpgAnti-QPRT Antibody Picoband® Western blot analysis of QPRT using anti-QPRT antibody (A09896). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QPRT antigen affinity purified polyclonal antibody (Catalog # A09896) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for QPRT at approximately 31-35 kDa. The expected band size for QPRT is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09896-qprt-primary-antibodies-if-testing-2.jpgAnti-QPRT Antibody Picoband® IF analysis of QPRT using anti-QPRT antibody (A09896). QPRT was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-QPRT Antibody (A09896) overnight at 4°C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09896-qprt-primary-antibodies-fcm-testing-3.jpgAnti-QPRT Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-QPRT antibody (A09896). Overlay histogram showing Jurkat cells stained with A09896 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QPRT Antibody (A09896, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rab3gap1-picoband-trade-antibody-a04789-2-boster.html2026-03-17T05:14:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-wb-testing-1.jpgAnti-RAB3GAP1 Antibody Picoband® Western blot analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB3GAP1 antigen affinity purified polyclonal antibody (Catalog # A04789-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB3GAP1 at approximately 130 kDa. The expected band size for RAB3GAP1 is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-2.jpgAnti-RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-3.jpgAnti-RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-4.jpgAnti-RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-5.jpgAnti-RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-6.jpgAnti-RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-ihc-testing-7.jpgAnti-RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-if-testing-8.jpgAnti-RAB3GAP1 Antibody Picoband® IF analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-if-testing-9.jpgAnti-RAB3GAP1 Antibody Picoband® IF analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2). RAB3GAP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RAB3GAP1 Antibody (A04789-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04789-2-rab3gap1-primary-antibodies-if-testing-10_2.jpgAnti-RAB3GAP1 Antibody Picoband® IF analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (A04789-2) and Anti-Beta Tubulin Antibody (M01857-3). RAB3GAP1 was detected in an immunocytochemical section of Siha cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB3GAP1 Antibody (A04789-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03302-2-asns-primary-antibodies-fcm-testing-11.jpgAnti-RAB3GAP1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RAB3GAP1 antibody (A04789-2). Overlay histogram showing HL-60 cells stained with A04789-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB3GAP1 Antibody (A04789-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cytokeratin-1-krt1-picoband-trade-antibody-a01639-1-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01639-1-krt1-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 1/Krt1 Antibody Picoband® Western blot analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody (A01639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 1/Krt1 antigen affinity purified polyclonal antibody (Catalog # A01639-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 1/Krt1 at approximately 66 kDa. The expected band size for Cytokeratin 1/Krt1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01639-1-krt1-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 1/Krt1 Antibody Picoband® IHC analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody (A01639-1). Cytokeratin 1/Krt1 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytokeratin 1/Krt1 Antibody (A01639-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01639-1-krt1-primary-antibodies-if-testing-3.jpgAnti-Cytokeratin 1/Krt1 Antibody Picoband® IF analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody (A01639-1). Cytokeratin 1/Krt1 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin 1/Krt1 Antibody (A01639-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ptrf-cavin1-picoband-trade-antibody-a31732-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-wb-testing-1.jpgAnti-PTRF/CAVIN1 Antibody Picoband® Western blot analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTRF/CAVIN1 antigen affinity purified polyclonal antibody (Catalog # A31732-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTRF/CAVIN1 at approximately 50-55 kDa. The expected band size for PTRF/CAVIN1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-2.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-3.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-4.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-5.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-6.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-7.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-8.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-9.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-10.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-11.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-ihc-testing-12.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IHC analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-if-testing-13.jpgAnti-PTRF/CAVIN1 Antibody Picoband® IF analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-yongqiu_zhen.pngAnti-PTRF/CAVIN1 Antibody Picoband®IF analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). PTRF/CAVIN1 was detected in an immunocytochemical section of Normal myocardium and alcohol-treated myocardium tissues. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTRF/CAVIN1 Antibody (A31732-2) overnight at 4°C. DyLight 488 Goat Anti-Rabbit IgG (H+L) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31732-2-cavin1-primary-antibodies-fcm-testing-14.jpgAnti-PTRF/CAVIN1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PTRF/CAVIN1 antibody (A31732-2). Overlay histogram showing SiHa cells stained with A31732-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTRF/CAVIN1 Antibody (A31732-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-lipin-1-lpin1-picoband-trade-antibody-a02467-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02467-2-lpin1-primary-antibodies-wb-testing-1.jpgAnti-Lipin 1/LPIN1 Antibody Picoband® Western blot analysis of Lipin 1/LPIN1 using anti-Lipin 1/LPIN1 antibody (A02467-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipin 1/LPIN1 antigen affinity purified polyclonal antibody (Catalog # A02467-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipin 1/LPIN1 at approximately 130 kDa. The expected band size for Lipin 1/LPIN1 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02467-2-lpin1-primary-antibodies-if-testing-2.jpgAnti-Lipin 1/LPIN1 Antibody Picoband® IF analysis of Lipin 1/LPIN1 using anti-Lipin 1/LPIN1 antibody (A02467-2). Lipin 1/LPIN1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Lipin 1/LPIN1 Antibody (A02467-2) overnight at 4°C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02467-2-lpin1-primary-antibodies-fcm-testing-3.jpgAnti-Lipin 1/LPIN1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-Lipin 1/LPIN1 antibody (A02467-2). Overlay histogram showing SiHa cells stained with A02467-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lipin 1/LPIN1 Antibody (A02467-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mdh1-picoband-trade-antibody-a04262-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04262-2-mdh1-primary-antibodies-wb-testing-1.jpgAnti-MDH1 Antibody Picoband® Western blot analysis of MDH1 using anti-MDH1 antibody (A04262-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDH1 antigen affinity purified polyclonal antibody (Catalog # A04262-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDH1 at approximately 36 kDa. The expected band size for MDH1 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04262-2-fnagi-17-1568337-g006.jpgAnti-MDH1 Antibody Picoband®Correlation analysis between pyroptosis-AD hub genes and immune cell infiltration. (A) Heatmap showed the correlation and p-values of 22 immune infiltrating cells and pyroptosis-related genes. The red indicated a positive correlation, whereas the blue represented a negative correlation, and p- values were shown as * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Correlation analysis between MDH1 and infiltrating immune cells. (C–H) Correlation scatter plots between the expression of MDH1 and immune cells presented significance. (I) Correlation analysis between FOXP3 and infiltrating immune cells. (J–O) Correlation scatter plots between the expression of MDH1 and immune cells presented significance. Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2025.1568337/full'>40438507</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04262-2-fnagi-17-1568337-g007.jpgAnti-MDH1 Antibody Picoband®Validation of the pyroptosis-AD hub genes at the level of RNA and protein in AD mice. (A) A hierarchical clustering heatmap based on the normalized expression of the five pyroptosis-AD genes in the combined dataset. (B) A clustering heatmap was constructed based on the normalized expression of the five pyroptosis-AD genes in the 6- and 12-month-old APP/PS1 and control mice. The 6- and 12-month-old APP/PS1 or WT mice were abbreviated as A6 and A12 or W6 and W12, respectively. (C–G) qPCR validation of mRNA expression of the pyroptosis-AD hub genes (Chmp2a, Egfr, Pkn2, Hsp90b1, and Mdh1, respectively) between the 12 months APP/PS1 and wild-type (WT) mice. Data are mean ± SEM ( n = 6 for WT, and n = 5 for APP/PS1 mice group, * p < 0.05, ** p < 0.01, unpaired two-tailed t -test). (H–K) The cell lysates from the hippocampus of APP/PS1 and WT mice were prepared and blotted with anti-Egfr, Pkn2, Hsp90b1, and Mdh1, respectively (up). The relative protein expressions of Egfr, Pkn2, Hsp90b1, and Mdh1 were calculated using Gapdh as an internal reference (below). Data are mean ± SEM ( n = 6 per group, * p < 0.05, ** p < 0.01, unpaired two-tailed t -test). Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2025.1568337/full'>40438507</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04262-2-fnagi-17-1568337-g008.jpgAnti-MDH1 Antibody Picoband®Screening and validation of candidate PRGs for the diagnosis of AD. (A) The ROC curve shows the diagnostic performance of the five feature genes in the combined dataset (training set). (B,C) ROC curves showing the diagnostic performance in the hippocampus of datasets GSE5281 (B) and GSE48350 (C) . (D–G) ROC curves show the diagnostic performance in the validation sets (entorhinal cortex of GSE5281, the hippocampus, or the temporal cortex data of GSE36980). (H,I) Differential expression of PKN2 and MDH1 in the GSE36980 (* p < 0.05, ** p < 0.01, unpaired two-tailed t -test). Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2025.1568337/full'>40438507</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04262-2-fnagi-17-1568337-g009.jpgAnti-MDH1 Antibody Picoband®Construction of lncRNA regulatory network of the pyroptosis-AD hub genes. (A) PCA of lncRNAs expression profiles of the APP/PS1 and WT mice at the age of 3, 6, and 12 months. (B) Visualization of the clustered volcano diagram for the DElncRs from six different comparisons, including APP/PS1 mice vs. WT mice at the age of 3, 6, and 12 months and comparison of APP/PS1 mice between different ages. (C) A hierarchical clustering heatmap based on the normalized expression in all samples of DElncRs. The 3-, 6-, and 12-month-old APP/PS1 or WT mice were abbreviated as A3, A6, and A12 or W3, W6, and W12, respectively. (D) The clustered heatmap was produced based on the membership scores of the six clusters obtained by time series analysis. All the DElncRs and five pyroptosis-AD hub genes were clustered into six groups. (E) Line charts showed the relative expression trend in each cluster. The five pyroptosis-AD hub genes were divided into cluster 2 (Champ2 and Mdh1), cluster 3 (Pkn2), and cluster (Egfr and Hsp90b1). The horizontal axis represents a total of nine samples in the age 3-, 6-, and 12-month groups in turn. (F) The heatmaps of correlation analysis of the five pyroptosis-AD hub genes and DElncRs. (G) Regulatory networks constructed by the five pyroptosis-AD hub genes and their top10 (show all if the numbers of lncRNA less than 10) correlated lncRNAs (the ID of lncRNAs could be queried in the NONCODE, NCBI, or Ensemble databases). Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2025.1568337/full'>40438507</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04262-2-mdh1-primary-antibodies-fcm-testing-2.jpgAnti-MDH1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-MDH1 antibody (A04262-2). Overlay histogram showing HL-60 cells stained with A04262-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MDH1 Antibody (A04262-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mtap-picoband-trade-antibody-a05448-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05448-2-mtap-primary-antibodies-wb-testing-1.jpgAnti-MTAP Antibody Picoband® Western blot analysis of MTAP using anti-MTAP antibody (A05448-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTAP antigen affinity purified polyclonal antibody (Catalog # A05448-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTAP at approximately 31 kDa. The expected band size for MTAP is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05448-2-mtap-primary-antibodies-if-testing-2.jpgAnti-MTAP Antibody Picoband® IF analysis of MTAP using anti-MTAP antibody (A05448-2). MTAP was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MTAP Antibody (A05448-2) overnight at 4°C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05448-2-mtap-primary-antibodies-fcm-testing-3.jpgAnti-MTAP Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-MTAP antibody (A05448-2). Overlay histogram showing U20S cells stained with A05448-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTAP Antibody (A05448-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nlrx1-picoband-trade-antibody-a04980-3-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04980-3-nlrx1-primary-antibodies-wb-testing-1.jpgAnti-NLRX1 Antibody Picoband® Western blot analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRX1 antigen affinity purified polyclonal antibody (Catalog # A04980-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRX1 at approximately 108 kDa. The expected band size for NLRX1 is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04980-3-nlrx1-primary-antibodies-ihc-testing-2.jpgAnti-NLRX1 Antibody Picoband® IHC analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). NLRX1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRX1 Antibody (A04980-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04980-3-nlrx1-primary-antibodies-ihc-testing-3.jpgAnti-NLRX1 Antibody Picoband® IHC analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). NLRX1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRX1 Antibody (A04980-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04980-3-nlrx1-primary-antibodies-ihc-testing-4.jpgAnti-NLRX1 Antibody Picoband® IHC analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). NLRX1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRX1 Antibody (A04980-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04980-3-nlrx1-primary-antibodies-if-testing-5.jpgAnti-NLRX1 Antibody Picoband® IF analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). NLRX1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NLRX1 Antibody (A04980-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04980-3-nlrx1-primary-antibodies-fcm-testing-7.jpgAnti-NLRX1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-NLRX1 antibody (A04980-3). Overlay histogram showing HL-60 cells stained with A04980-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRX1 Antibody (A04980-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04980-3-nlrx1-primary-antibodies-if-testing-6.jpgAnti-NLRX1 Antibody Picoband® IF analysis of NLRX1 using anti-NLRX1 antibody (A04980-3). NLRX1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NLRX1 Antibody (A04980-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-nqo1-picoband-trade-antibody-a00494-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00494-2-nqo1-primary-antibodies-wb-testing-1.jpgAnti-NQO1 Antibody Picoband® Western blot analysis of NQO1 using anti-NQO1 antibody (A00494-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (Catalog # A00494-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 31 kDa. The expected band size for NQO1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00494-2-nqo1-primary-antibodies-ihc-testing-1.jpgAnti-NQO1 Antibody Picoband®IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2). NQO1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NQO1 Antibody (A00494-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00494-2-nqo1-primary-antibodies-ihc-testing-2.jpgAnti-NQO1 Antibody Picoband®IHC analysis of NQO1 using anti-NQO1 antibody (A00494-2). NQO1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NQO1 Antibody (A00494-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00494-2-nqo1-primary-antibodies-if-testing-2.jpgAnti-NQO1 Antibody Picoband® IF analysis of NQO1 using anti-NQO1 antibody (A00494-2). NQO1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NQO1 Antibody (A00494-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00494-2-nqo1-primary-antibodies-fcm-testing-3.jpgAnti-NQO1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-NQO1 antibody (A00494-2). Overlay histogram showing MCF-7 cells stained with A00494-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NQO1 Antibody (A00494-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tab3-picoband-trade-antibody-a05084-3-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05084-3-tab3-primary-antibodies-wb-testing-1.jpgAnti-TAB3 Antibody Picoband® Western blot analysis of TAB3 using anti-TAB3 antibody (A05084-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB3 antigen affinity purified polyclonal antibody (Catalog # A05084-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB3 at approximately 90 kDa. The expected band size for TAB3 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05084-3-tab3-primary-antibodies-fcm-testing-2.jpgAnti-TAB3 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-TAB3 antibody (A05084-3). Overlay histogram showing HL-60 cells stained with A05084-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAB3 Antibody (A05084-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05084-3-tab3-primary-antibodies-fcm-testing-3.jpgAnti-TAB3 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-TAB3 antibody (A05084-3). Overlay histogram showing HL-60 cells stained with A05084-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAB3 Antibody (A05084-3, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-dpys-picoband-trade-antibody-a09603-1-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09603-1-dpys-primary-antibodies-wb-testing-1.jpgAnti-DPYS Antibody Picoband® Western blot analysis of DPYS using anti-DPYS antibody (A09603-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYS antigen affinity purified polyclonal antibody (Catalog # A09603-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DPYS at approximately 57 kDa. The expected band size for DPYS is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09603-1-dpys-primary-antibodies-fcm-testing-2.jpgAnti-DPYS Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-DPYS antibody (A09603-1). Overlay histogram showing HL-60 cells stained with A09603-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPYS Antibody (A09603-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-spock3-picoband-trade-antibody-a03151-1-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03151-1-spock3-primary-antibodies-wb-testing-1.jpgAnti-SPOCK3 Antibody Picoband® Western blot analysis of SPOCK3 using anti-SPOCK3 antibody (A03151-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOCK3 antigen affinity purified polyclonal antibody (Catalog # A03151-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPOCK3 at approximately 57 kDa. The expected band size for SPOCK3 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03151-1-spock3-primary-antibodies-if-testing-2.jpgAnti-SPOCK3 Antibody Picoband® IF analysis of SPOCK3 using anti-SPOCK3 antibody (A03151-1). SPOCK3 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPOCK3 Antibody (A03151-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-atg4b-picoband-trade-antibody-a02885-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02885-2-atg4b-primary-antibodies-wb-testing-1.jpgAnti-ATG4B Antibody Picoband® Western blot analysis of ATG4B using anti-ATG4B antibody (A02885-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4B antigen affinity purified polyclonal antibody (Catalog # A02885-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG4B at approximately 48-50 kDa. The expected band size for ATG4B is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02885-2-atg4b-primary-antibodies-fcm-testing-2.jpgAnti-ATG4B Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ATG4B antibody (A02885-2). Overlay histogram showing RT4 cells stained with A02885-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG4B Antibody (A02885-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ceacam1-picoband-trade-antibody-a00923-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00923-2-ceacam1-primary-antibodies-wb-testing-1.jpgAnti-CEACAM1 Antibody Picoband® Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 120-150 kDa. The expected band size for CEACAM1 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-gab2-picoband-trade-antibody-a02386-3-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02386-3-gab2-primary-antibodies-wb-testing-1.jpgAnti-GAB2 Antibody Picoband® Western blot analysis of GAB2 using anti-GAB2 antibody (A02386-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAB2 antigen affinity purified polyclonal antibody (Catalog # A02386-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAB2 at approximately 90 kDa. The expected band size for GAB2 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02386-3-gab2-primary-antibodies-fcm-testing-2.jpgAnti-GAB2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-GAB2 antibody (A02386-3). Overlay histogram showing HL-60 cells stained with A02386-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAB2 Antibody (A02386-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02386-3-gab2-primary-antibodies-fcm-testing-3.jpgAnti-GAB2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-GAB2 antibody (A02386-3). Overlay histogram showing HL-60 cells stained with A02386-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAB2 Antibody (A02386-3, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02386-3-gab2-primary-antibodies-fcm-testing-4.jpgAnti-GAB2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-GAB2 antibody (A02386-3). Overlay histogram showing THP-1 cells stained with A02386-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAB2 Antibody (A02386-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-lin28b-picoband-trade-antibody-a00896-1-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00896-1-lin28b-primary-antibodies-wb-testing-1.jpgAnti-LIN28B Antibody Picoband® Western blot analysis of LIN28B using anti-LIN28B antibody (A00896-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN28B antigen affinity purified polyclonal antibody (Catalog # A00896-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIN28B at approximately 35 kDa. The expected band size for LIN28B is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00896-1-lin28b-primary-antibodies-ihc-testing-2.jpgAnti-LIN28B Antibody Picoband® IHC analysis of LIN28B using anti-LIN28B antibody (A00896-1). LIN28B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIN28B Antibody (A00896-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-rbms1-picoband-trade-antibody-a08752-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-wb-testing-1.jpgAnti-RBMS1 Antibody Picoband® Western blot analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMS1 antigen affinity purified polyclonal antibody (Catalog # A08752-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBMS1 at approximately 50 kDa. The expected band size for RBMS1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-2.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-3.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-4.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-5.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-6.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-7.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-8.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-9.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-ihc-testing-10.jpgAnti-RBMS1 Antibody Picoband® IHC analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-if-testing-11.jpgAnti-RBMS1 Antibody Picoband® IF analysis of RBMS1 using anti-RBMS1 antibody (A08752-2). RBMS1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBMS1 Antibody (A08752-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08752-2-rbms1-primary-antibodies-fcm-testing-12.jpgAnti-RBMS1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-RBMS1 antibody (A08752-2). Overlay histogram showing U87 cells stained with A08752-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBMS1 Antibody (A08752-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rab34-picoband-trade-antibody-a11097-3-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11097-3-rab34-primary-antibodies-wb-testing-1_1.jpgAnti-RAB34 Antibody Picoband® Western blot analysis of RAB34 using anti-RAB34 antibody (A11097-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB34 antigen affinity purified polyclonal antibody (Catalog # A11097-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB34 at approximately 29 kDa. The expected band size for RAB34 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11097-3-rab34-primary-antibodies-fcm-testing-2_1.jpgAnti-RAB34 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-RAB34 antibody (A11097-3). Overlay histogram showing A549 cells stained with A11097-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB34 Antibody (A11097-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rab36-picoband-trade-antibody-a14616-1-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14616-1-rab36-primary-antibodies-wb-testing-1.jpgAnti-RAB36 Antibody Picoband® Western blot analysis of RAB36 using anti-RAB36 antibody (A14616-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse ovary tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB36 antigen affinity purified polyclonal antibody (Catalog # A14616-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB36 at approximately 36 kDa. The expected band size for RAB36 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-alg3-picoband-trade-antibody-a04688-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04688-alg3-primary-antibodies-wb-testing-1.jpgAnti-ALG3 Antibody Picoband® Western blot analysis of ALG3 using anti-ALG3 antibody (A04688). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALG3 antigen affinity purified polyclonal antibody (Catalog # A04688) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALG3 at approximately 50-55 kDa. The expected band size for ALG3 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04688-alg3-primary-antibodies-fcm-testing-2.jpgAnti-ALG3 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-ALG3 antibody (A04688). Overlay histogram showing THP-1 cells stained with A04688 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALG3 Antibody (A04688, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04688-alg3-primary-antibodies-fcm-testing-3.jpgAnti-ALG3 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ALG3 antibody (A04688). Overlay histogram showing RT4 cells stained with A04688 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALG3 Antibody (A04688, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cdnf-picoband-trade-antibody-a10069-2-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10069-2-cdnf-primary-antibodies-wb-testing-1.jpgAnti-CDNF Antibody Picoband® Western blot analysis of Cdnf using anti-Cdnf antibody (A10069-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdnf antigen affinity purified polyclonal antibody (Catalog # A10069-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdnf at approximately 19-20 kDa. The expected band size for Cdnf is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10069-2-cdnf-primary-antibodies-fcm-testing-2.jpgAnti-CDNF Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Cdnf antibody (A10069-2). Overlay histogram showing HEPA1-6 cells stained with A10069-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cdnf Antibody (A10069-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dusp4-antibody-dz41233-boster.html2026-03-17T05:14:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41233-dusp4-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish dusp4 Antibody IHC analysis of Dusp4 using anti-Dusp4 antibody (DZ41233). Dusp4 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Dusp4 Antibody (DZ41233) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41233-dusp4-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish dusp4 Antibody IHC analysis of Dusp4 using anti-Dusp4 antibody (DZ41233). Dusp4 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit Dusp4 Antibody (DZ41233) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tmem38b-antibody-dz41218-1-boster.html2026-03-17T05:14:19+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquito-clipe5-antibody-dz41335-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-ssp3-antibody-dz41327-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mylpfa-antibody-dz41336-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41336-mylpfa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Mylpfa AntibodyWestern blot analysis of Mylpfa using anti-Mylpfa antibody (DZ41336). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mylpfa antigen affinity purified polyclonal antibody (DZ41336) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Mylpfa at approximately 19 kDa. The expected band size for Mylpfa is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41336-mylpfa-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Mylpfa Antibody IHC analysis of Mylpfa using anti-Mylpfa antibody (DZ41336). Mylpfa was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mylpfa Antibody (DZ41336) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41336-mylpfa-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Mylpfa Antibody IHC analysis of Mylpfa using anti-Mylpfa antibody (DZ41336). Mylpfa was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mylpfa Antibody (DZ41336) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-human-lilrb4-antibody-dz41337-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-slime-mold-cpna-antibody-dz41344-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-slime-mold-cpnc-antibody-dz41345-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-dttc28-cg43163-antibody-dz41354-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-fsn-cg4643-antibody-dz41355-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-endothelin-1-antibody-dz41358-boster.html2026-03-17T05:14:19+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-col6a2-antibody-dz41356-boster.html2026-04-01T05:01:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41356-col6a2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Col6a2 Antibody IHC analysis of Col6a2 using anti-Col6a2 antibody (DZ41356). Col6a2 was detected in a paraffin-embedded section of Zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Col6a2 Antibody (DZ41356) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41356-col6a2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Col6a2 Antibody IHC analysis of Col6a2 using anti-Col6a2 antibody (DZ41356). Col6a2 was detected in a paraffin-embedded section of Zebrafish skeletal mucsle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Col6a2 Antibody (DZ41356) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-gnu-antibody-dz41359-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-mouse-ear-cress-at4g17080-antibody-dz41363-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-phactr1-antibody-dz41368-boster.html2026-03-17T05:14:19+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-e-coli-foca-antibody-dz41361-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-casmp1-antibody-dz41364-boster.html2026-03-10T04:35:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-parasteatoda-tepidariorim-wnt8-antibody-dz41366-boster.html2026-03-24T05:36:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-parasteatoda-tepidariorim-caudal-antibody-dz41365-boster.html2026-03-24T05:36:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-human-c2orf69-antibody-dz41220-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vwf-antibody-dz41225-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41225-vwf-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish vwf Antibody IHC analysis of Vwf using anti-Vwf antibody (DZ41225). Vwf was detected in a paraffin-embedded section of Zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vwf Antibody (DZ41225) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41225-vwf-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish vwf Antibody IHC analysis of Vwf using anti-Vwf antibody (DZ41225). Vwf was detected in a paraffin-embedded section of Zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vwf Antibody (DZ41225) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41225-vwf-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish vwf Antibody IHC analysis of Vwf using anti-Vwf antibody (DZ41225). Vwf was detected in a paraffin-embedded section of Zebrafish gill tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vwf Antibody (DZ41225) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41225-vwf-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish vwf Antibody IHC analysis of Vwf using anti-Vwf antibody (DZ41225). Vwf was detected in a paraffin-embedded section of Zebrafish gill tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vwf Antibody (DZ41225) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-crithidia-fasciculata-pde1-antibody-dz41322-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-woodchuck-hepatitis-b-virus-isolate-8-whv-protein-x-antibody-dz41339-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-slime-mold-pata-antibody-dz41342-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-buc2l-antibody-dz41348-boster.html2026-03-17T05:14:19+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-english-walnut-jrppo2-antibody-dz41352-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-yellowfever-mosquito-ecr-antibody-dz41362-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sim1a-antibody-dz41372-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41372-sim1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish sim1a Antibody Western blot analysis of Sim1a using anti-Sim1a antibody (DZ41372). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Marker, Lane 2: Zebrafish tissue lysates, Lane 3: Zebrafish head tissue lysates, Lane 4: Zebrafish body tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sim1a antigen affinity purified polyclonal antibody (DZ41372) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sim1a at approximately 82 kDa. The expected band size for Sim1a is at 82 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-calb2-antibody-dz41373-boster.html2026-03-17T05:14:19+00:00daily0.9 https://www.bosterbio.com/picokine-elisa-kits/human-gusb-picokine-elisa-kit-ek2214-boster.html2026-03-10T04:35:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2214.jpgHuman GUSB ELISA Kit PicoKine®Human GUSB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hexa-picokine-elisa-kit-ek2215-boster.html2026-03-10T04:35:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2215.jpgHuman HEXA ELISA Kit PicoKine®Human HEXA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hnmt-picokine-elisa-kit-ek2216-boster.html2026-03-10T04:35:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2216.pngHuman HNMT ELISA Kit PicoKine®Human HNMT PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sdf-1-cxcl12-picokine-elisa-kit-ek0499-boster.html2026-03-10T04:35:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0499.jpgHuman SDF-1/CXCL12 ELISA Kit PicoKine®Human SDF-1/CXCL12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-saccharomyces-cerevisiae-elp3-antibody-dz41382-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-si-dkeyp-75b4-10-antibody-dz41383-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41383-sidkeyp-75b4.10-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish si:dkeyp-75b4.10/eslec Antibody Western blot analysis of Si:dkeyp-75b4.10 using anti-Si:dkeyp-75b4.10 antibody (DZ41383). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Marker, Lane 2: Zebrafish, Lane 3: Zebrafish body tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Si:dkeyp-75b4.10 antigen affinity purified polyclonal antibody (DZ41383) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Si:dkeyp-75b4.10at approximately 18 kDa. The expected band size for Si:dkeyp-75b4.10 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41383-sidkeyp-75b4.10-custom-antibodies-if-testing-2_1.jpgAnti-Zebrafish si:dkeyp-75b4.10/eslec Antibody IF analysis of zebrafish eosinophils using Anti-Zebrafish Si:Dkeyp-75b4.10 Antibody (DZ41383). Si:Dkeyp-75b4.10 was detected in zebrafish eosinophils in FFPE tissue sections and fixed cells. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 3% goat serum in PBS. The tissue section was then incubated with 4 μg/mL rabbit anti-GFAP antibody (MA1045) and anti-MBP antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126), Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:1000 dilution and incubated for 1 hour at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-human-prdm11-antibody-dz41385-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-human-prdm11-antibody-dz41386-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-edc3-antibody-dz41387-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-fruit-fly-capu-antibody-dz41389-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-nicotiana-tabacum-nitab4-5-0001937g0020-1-antibody-dz41392-boster.html2026-03-10T04:35:15+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-oryzias-latipes-ccl25b-antibody-dz41393-boster.html2026-03-10T04:35:16+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-mouse-ear-cress-nup50c-antibody-dz41394-boster.html2026-03-10T04:35:16+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-mouse-ear-cress-seh1-antibody-dz41395-boster.html2026-03-10T04:35:16+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tmem242-antibody-dz41378-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41378-tmem242-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TMEM242 Antibody IHC analysis of TMEM242 using anti-TMEM242 antibody (DZ41378). TMEM242 was detected in a paraffin-embedded section of zebrafish intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM242 Antibody (DZ41378) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-african-malaria-mosquitorel2-antibody-dz41380-boster.html2026-03-10T04:35:16+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-itga8-picoband-trade-antibody-a06636-2-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06636-2-itga8-primary-antibodies-wb-testing-1.jpgAnti-ITGA8 Antibody Picoband® Western blot analysis of ITGA8 using anti-ITGA8 antibody (A06636-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA8 antigen affinity purified polyclonal antibody (Catalog # A06636-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA8 at approximately 180 kDa. The expected band size for ITGA8 is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06636-2-itga8-primary-antibodies-if-testing-2.jpgAnti-ITGA8 Antibody Picoband® IF analysis of ITGA8 using anti-ITGA8 antibody (A06636-2). ITGA8 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ITGA8 Antibody (A06636-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06636-2-itga8-primary-antibodies-fcm-testing-3.jpgAnti-ITGA8 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-ITGA8 antibody (A06636-2). Overlay histogram showing 293T cells stained with A06636-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ITGA8 Antibody (A06636-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ehd4-picoband-trade-antibody-a07430-1-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07430-1-ehd4-primary-antibodies-wb-testing-1.jpgAnti-EHD4 Antibody Picoband® Western blot analysis of EHD4 using anti-EHD4 antibody (A07430-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey heart tissue lysates, Lane 4: human placenta tissue lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD4 antigen affinity purified polyclonal antibody (Catalog # A07430-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD4 at approximately 70 kDa. The expected band size for EHD4 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07430-1-ehd4-primary-antibodies-wb-testing-2.jpgAnti-EHD4 Antibody Picoband® Western blot analysis of EHD4 using anti-EHD4 antibody (A07430-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHD4 antigen affinity purified polyclonal antibody (Catalog # A07430-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EHD4 at approximately 70 kDa. The expected band size for EHD4 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07430-1-ehd4-primary-antibodies-fcm-testing-3.jpgAnti-EHD4 Antibody Picoband® Flow Cytometry analysis of U2OS cells using anti-EHD4 antibody (A07430-1). Overlay histogram showing U20S cells stained with A07430-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD4 Antibody (A07430-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07430-1-ehd4-primary-antibodies-fcm-testing-4.jpgAnti-EHD4 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-EHD4 antibody (A07430-1). Overlay histogram showing U251 cells stained with A07430-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD4 Antibody (A07430-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-naf1-picoband-trade-antibody-a02629-1-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02629-1-naf1-primary-antibodies-wb-testing-1.jpgAnti-NAF1 Antibody Picoband® Western blot analysis of NAF1 using anti-NAF1 antibody (A02629-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAF1 antigen affinity purified polyclonal antibody (Catalog # A02629-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAF1 at approximately 70 kDa. The expected band size for NAF1 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-nesprin3-syne3-picoband-trade-antibody-a13164-2-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13164-2-syne3-primary-antibodies-wb-testing-1.jpgAnti-Nesprin3/SYNE3 Antibody Picoband® Western blot analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin3/SYNE3 antigen affinity purified polyclonal antibody (Catalog # A13164-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nesprin3/SYNE3 at approximately 100 kDa. The expected band size for Nesprin3/SYNE3 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13164-2-syne3-primary-antibodies-ihc-testing-2.jpgAnti-Nesprin3/SYNE3 Antibody Picoband® IHC analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Nesprin3/SYNE3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nesprin3/SYNE3 Antibody (A13164-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13164-2-syne3-primary-antibodies-ihc-testing-3.jpgAnti-Nesprin3/SYNE3 Antibody Picoband® IHC analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Nesprin3/SYNE3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nesprin3/SYNE3 Antibody (A13164-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13164-2-syne3-primary-antibodies-ihc-testing-4.jpgAnti-Nesprin3/SYNE3 Antibody Picoband® IHC analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Nesprin3/SYNE3 was detected in a paraffin-embedded section of human spleen rupture tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nesprin3/SYNE3 Antibody (A13164-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13164-2-syne3-primary-antibodies-ihc-testing-5.jpgAnti-Nesprin3/SYNE3 Antibody Picoband® IHC analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Nesprin3/SYNE3 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nesprin3/SYNE3 Antibody (A13164-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13164-2-syne3-primary-antibodies-fcm-testing-6.jpgAnti-Nesprin3/SYNE3 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-Nesprin3/SYNE3 antibody (A13164-2). Overlay histogram showing U87 cells stained with A13164-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin3/SYNE3 Antibody (A13164-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-quiescin-q6-qsox1-picoband-trade-antibody-a04549-1-boster.html2026-03-17T05:14:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04549-1-qsox1-primary-antibodies-wb-testing-1.jpgAnti-Quiescin Q6/QSOX1 Antibody Picoband® Western blot analysis of Quiescin Q6/QSOX1 using anti-Quiescin Q6/QSOX1 antibody (A04549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Quiescin Q6/QSOX1 antigen affinity purified polyclonal antibody (Catalog # A04549-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Quiescin Q6/QSOX1 at approximately 105 kDa. The expected band size for Quiescin Q6/QSOX1 is at 83 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-mcp2-ccl8-picoband-trade-antibody-a03237-2-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03237-2-ccl8-primary-antibodies-wb-testing-1.jpgAnti-MCP2/CCL8 Antibody Picoband® Western blot analysis of MCP2/CCL8 using anti-MCP2/CCL8 antibody (A03237-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat small intestine tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP2/CCL8 antigen affinity purified polyclonal antibody (Catalog # A03237-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP2/CCL8 at approximately 22 kDa. The expected band size for MCP2/CCL8 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-chromogranin-a-chga-picoband-trade-antibody-a01256-4-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01256-4-chga-primary-antibodies-wb-testing-1.jpgAnti-Chromogranin A/CHGA Antibody Picoband® Western blot analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CHGA antigen affinity purified polyclonal antibody (Catalog # A01256-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CHGA at approximately 70 kDa. The expected band size for Chromogranin A/CHGA is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01256-4-chga-primary-antibodies-fcm-testing-5.jpgAnti-Chromogranin A/CHGA Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Chromogranin A/CHGA antibody (A01256-4). Overlay histogram showing MCF-7 cells stained with A01256-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Chromogranin A/CHGA Antibody (A01256-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01256-4-chga-primary-antibodies-ihc-testing-2_1.jpgAnti-Chromogranin A/CHGA Antibody Picoband® IHC analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Chromogranin A/CHGA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chromogranin A/CHGA Antibody (A01256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01256-4-chga-primary-antibodies-ihc-testing-3_1.jpgAnti-Chromogranin A/CHGA Antibody Picoband® IHC analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Chromogranin A/CHGA was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chromogranin A/CHGA Antibody (A01256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01256-4-chga-primary-antibodies-ihc-testing-4_1.jpgAnti-Chromogranin A/CHGA Antibody Picoband® IHC analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Chromogranin A/CHGA was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Chromogranin A/CHGA Antibody (A01256-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-crkl-picoband-trade-antibody-a02100-1-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02100-1-crkl-primary-antibodies-wb-testing-1.jpgAnti-CRKL Antibody Picoband® Western blot analysis of CRKL using anti-CRKL antibody (A02100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRKL antigen affinity purified polyclonal antibody (Catalog # A02100-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRKL at approximately 35 kDa. The expected band size for CRKL is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02100-1-crkl-primary-antibodies-if-testing-2.jpgAnti-CRKL Antibody Picoband® IF analysis of CRKL using anti-CRKL antibody (A02100-1) and anti-Tubulin Alpha antibody (M03989-3). CRKL was detected in immunocytochemical section of U87 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CRKL Antibody (A02100-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02100-1-crkl-primary-antibodies-ip-testing-1.jpgAnti-CRKL Antibody Picoband®Immunoprecipitating (IP) CRKL in 293T whole cell lysate. Western blot analysis of CRKL using anti-CRKL antibody (A02100-1); Lane 1: 293T whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-CRKL antibody in 293T whole cell lysate; Lane 3: anti-CRKL antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CRKL antigen affinity purified polyclonal antibody (A02100-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CRKL at approximately 34 kDa. The expected band size for CRKL is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02100-1-crkl-primary-antibodies-fcm-testing-3.jpgAnti-CRKL Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-CRKL antibody (A02100-1). Overlay histogram showing RT4 cells stained with A02100-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRKL Antibody (A02100-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cblif-picoband-trade-antibody-a00038-1-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00038-1-cblif-primary-antibodies-wb-testing-1.jpgAnti-CBLIF Antibody Picoband® Western blot analysis of CBLIF using anti-CBLIF antibody (A00038-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HGC-27 whole cell lysates, Lane 2: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBLIF antigen affinity purified polyclonal antibody (Catalog # A00038-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBLIF at approximately 52 kDa. The expected band size for CBLIF is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00038-1-cblif-primary-antibodies-if-testing-2.jpgAnti-CBLIF Antibody Picoband® IF analysis of CBLIF using anti-CBLIF antibody (A00038-1). CBLIF was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CBLIF Antibody (A00038-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00038-1-cblif-primary-antibodies-fcm-testing-3.jpgAnti-CBLIF Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-CBLIF antibody (A00038-1). Overlay histogram showing U937 cells stained with A00038-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBLIF Antibody (A00038-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tap1-picoband-trade-antibody-a01123-2-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01123-2-tap1-primary-antibodies-wb-testing-1.jpgAnti-Tap1 Antibody Picoband® Western blot analysis of Tap1 using anti-Tap1 antibody (A01123-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tap1 antigen affinity purified polyclonal antibody (Catalog # A01123-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tap1 at approximately 70 kDa. The expected band size for Tap1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01123-2-tap1-primary-antibodies-if-testing-2.jpgAnti-Tap1 Antibody Picoband® IF analysis of Tap1 using anti-Tap1 antibody (A01123-2). Tap1 was detected in an immunocytochemical section of RAW264.7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tap1 Antibody (A01123-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01123-2-tap1-primary-antibodies-fcm-testing-3.jpgAnti-Tap1 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Tap1 antibody (A01123-2). Overlay histogram showing HEPA1-6 cells stained with A01123-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tap1 Antibody (A01123-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-thrombomodulin-thbd-picoband-trade-antibody-a01325-3-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01325-3-thbd-primary-antibodies-wb-testing-1.jpgAnti-Thrombomodulin/THBD Antibody Picoband® Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (Catalog # A01325-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01325-3-thbd-primary-antibodies-ihc-testing-2.jpgAnti-Thrombomodulin/THBD Antibody Picoband® IHC analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Thrombomodulin/THBD was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thrombomodulin/THBD Antibody (A01325-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01325-3-thbd-primary-antibodies-ihc-testing-3.jpgAnti-Thrombomodulin/THBD Antibody Picoband® IHC analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Thrombomodulin/THBD was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Thrombomodulin/THBD Antibody (A01325-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01325-3-thbd-primary-antibodies-if-testing-4.jpgAnti-Thrombomodulin/THBD Antibody Picoband® IF analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3) and anti-Tubulin Alpha antibody (M03989-3). Thrombomodulin/THBD was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Thrombomodulin/THBD Antibody (A01325-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01325-3-thbd-primary-antibodies-fcm-testing-5.jpgAnti-Thrombomodulin/THBD Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Thrombomodulin/THBD antibody (A01325-3). Overlay histogram showing THP-1 cells stained with A01325-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thrombomodulin/THBD Antibody (A01325-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01325-3-thbd-primary-antibodies-ip-testing-6.jpgAnti-Thrombomodulin/THBD Antibody Picoband® Immunoprecipitating Thrombomodulin/THBD in A431 whole cell lysate. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Lane 1: A431 whole cell lysates (30ug) Lane 2: Rabbit control IgG instead of anti-Thrombomodulin/THBD antibody in A431 whole cell lysate. Lane 3: anti-Thrombomodulin/THBD antibody (2μg) + A431 whole cell lysate (500μg) After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (A01325-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-hamartin-tsc1-picoband-trade-antibody-a00365-2-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-wb-testing-1.jpgAnti-Hamartin/TSC1 Antibody Picoband® Western blot analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin/TSC1 antigen affinity purified polyclonal antibody (Catalog # A00365-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hamartin/TSC1 at approximately 160 kDa. The expected band size for Hamartin/TSC1 is at 130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-ihc-testing-2.jpgAnti-Hamartin/TSC1 Antibody Picoband® IHC analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Hamartin/TSC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hamartin/TSC1 Antibody (A00365-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-ihc-testing-3.jpgAnti-Hamartin/TSC1 Antibody Picoband® IHC analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Hamartin/TSC1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hamartin/TSC1 Antibody (A00365-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-ihc-testing-4.jpgAnti-Hamartin/TSC1 Antibody Picoband® IHC analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Hamartin/TSC1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hamartin/TSC1 Antibody (A00365-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-ihc-testing-5.jpgAnti-Hamartin/TSC1 Antibody Picoband® IHC analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Hamartin/TSC1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hamartin/TSC1 Antibody (A00365-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-ihc-testing-6.jpgAnti-Hamartin/TSC1 Antibody Picoband® IHC analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Hamartin/TSC1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hamartin/TSC1 Antibody (A00365-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-if-testing-7.jpgAnti-Hamartin/TSC1 Antibody Picoband® IF analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Hamartin/TSC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Hamartin/TSC1 Antibody (A00365-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00365-2-tsc1-primary-antibodies-fcm-testing-8.jpgAnti-Hamartin/TSC1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-Hamartin/TSC1 antibody (A00365-2). Overlay histogram showing THP-1 cells stained with A00365-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hamartin/TSC1 Antibody (A00365-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-c19orf2-uri1-picoband-trade-antibody-a05245-2-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05245-2-uri1-primary-antibodies-fcm-testing-2.jpgAnti-C19orf2/URI1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-C19orf2/URI1 antibody (A05245-2). Overlay histogram showing RT4 cells stained with A05245-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf2/URI1 Antibody (A05245-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05245-2-uri1-primary-antibodies-wb-testing-1.jpgAnti-C19orf2/URI1 Antibody Picoband® Western blot analysis of C19orf2/URI1 using anti-C19orf2/URI1 antibody (A05245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human HEL1 whole cell lysates, Lane 5: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf2/URI1 antigen affinity purified polyclonal antibody (Catalog # A05245-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf2/URI1 at approximately 80 kDa. The expected band size for C19orf2/URI1 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-wrs-wars1-picoband-trade-antibody-a02444-2-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02444-2-wars1-primary-antibodies-wb-testing-1.jpgAnti-WRS/WARS1 Antibody Picoband® Western blot analysis of WRS/WARS1 using anti-WRS/WARS1 antibody (A02444-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRS/WARS1 antigen affinity purified polyclonal antibody (Catalog # A02444-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRS/WARS1 at approximately 55 kDa. The expected band size for WRS/WARS1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02444-2-wars1-primary-antibodies-ihc-testing-2.jpgAnti-WRS/WARS1 Antibody Picoband® IHC analysis of WRS/WARS1 using anti-WRS/WARS1 antibody (A02444-2). WRS/WARS1 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WRS/WARS1 Antibody (A02444-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02444-2-wars1-primary-antibodies-ihc-testing-3.jpgAnti-WRS/WARS1 Antibody Picoband® IHC analysis of WRS/WARS1 using anti-WRS/WARS1 antibody (A02444-2). WRS/WARS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WRS/WARS1 Antibody (A02444-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02444-2-wars1-primary-antibodies-if-testing-4.jpgAnti-WRS/WARS1 Antibody Picoband® IF analysis of WRS/WARS1 using anti-WRS/WARS1 antibody (A02444-2). WRS/WARS1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WRS/WARS1 Antibody (A02444-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02444-2-wars1-primary-antibodies-fcm-testing-5.jpgAnti-WRS/WARS1 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-WRS/WARS1 antibody (A02444-2). Overlay histogram showing THP-1 cells stained with A02444-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WRS/WARS1 Antibody (A02444-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rad51c-picoband-trade-antibody-a01837-1-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01837-1-rad51c-primary-antibodies-wb-testing-1.jpgAnti-RAD51C Antibody Picoband® Western blot analysis of RAD51C using anti-RAD51C antibody (A01837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51C antigen affinity purified polyclonal antibody (Catalog # A01837-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAD51C at approximately 42 kDa. The expected band size for RAD51C is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01837-1-rad51c-primary-antibodies-ihc-testing-2.jpgAnti-RAD51C Antibody Picoband® IHC analysis of RAD51C using anti-RAD51C antibody (A01837-1). RAD51C was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD51C Antibody (A01837-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01837-1-rad51c-primary-antibodies-ihc-testing-3.jpgAnti-RAD51C Antibody Picoband® IHC analysis of RAD51C using anti-RAD51C antibody (A01837-1). RAD51C was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD51C Antibody (A01837-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01837-1-rad51c-primary-antibodies-ihc-testing-4.jpgAnti-RAD51C Antibody Picoband® IHC analysis of RAD51C using anti-RAD51C antibody (A01837-1). RAD51C was detected in a paraffin-embedded section of human larynx squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD51C Antibody (A01837-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01837-1-rad51c-primary-antibodies-ihc-testing-5.jpgAnti-RAD51C Antibody Picoband® IHC analysis of RAD51C using anti-RAD51C antibody (A01837-1). RAD51C was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD51C Antibody (A01837-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01837-1-rad51c-primary-antibodies-ihc-testing-6.jpgAnti-RAD51C Antibody Picoband® IHC analysis of RAD51C using anti-RAD51C antibody (A01837-1). RAD51C was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD51C Antibody (A01837-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01837-1-rad51c-primary-antibodies-fcm-testing-7.jpgAnti-RAD51C Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-RAD51C antibody (A01837-1). Overlay histogram showing RT4 cells stained with A01837-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD51C Antibody (A01837-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-p97-dap5-eif4g2-picoband-trade-antibody-a03888-2-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03888-2-eif4g2-primary-antibodies-wb-testing-1.jpgAnti-P97/DAP5/EIF4G2 Antibody Picoband® Western blot analysis of P97/DAP5/EIF4G2 using anti-P97/DAP5/EIF4G2 antibody (A03888-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P97/DAP5/EIF4G2 antigen affinity purified polyclonal antibody (Catalog # A03888-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P97/DAP5/EIF4G2 at approximately 97 kDa. The expected band size for P97/DAP5/EIF4G2 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03888-2-eif4g2-primary-antibodies-if-testing-2.jpgAnti-P97/DAP5/EIF4G2 Antibody Picoband® IF analysis of P97/DAP5/EIF4G2 using anti-P97/DAP5/EIF4G2 antibody (A03888-2). P97/DAP5/EIF4G2 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P97/DAP5/EIF4G2 Antibody (A03888-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03888-2-eif4g2-primary-antibodies-fcm-testing-3.jpgAnti-P97/DAP5/EIF4G2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-P97/DAP5/EIF4G2 antibody (A03888-2). Overlay histogram showing THP-1 cells stained with A03888-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P97/DAP5/EIF4G2 Antibody (A03888-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rbp1-picoband-trade-antibody-a03820-4-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-wb-testing-1.jpgAnti-RBP1 Antibody Picoband® Western blot analysis of RBP1 using anti-RBP1 antibody (A03820-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP1 antigen affinity purified polyclonal antibody (Catalog # A03820-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBP1 at approximately 16 kDa. The expected band size for RBP1 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-ihc-testing-2.jpgAnti-RBP1 Antibody Picoband® IHC analysis of RBP1 using anti-RBP1 antibody (A03820-4). RBP1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBP1 Antibody (A03820-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-ihc-testing-3.jpgAnti-RBP1 Antibody Picoband® IHC analysis of RBP1 using anti-RBP1 antibody (A03820-4). RBP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBP1 Antibody (A03820-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-ihc-testing-4.jpgAnti-RBP1 Antibody Picoband® IHC analysis of RBP1 using anti-RBP1 antibody (A03820-4). RBP1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBP1 Antibody (A03820-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-ihc-testing-5.jpgAnti-RBP1 Antibody Picoband® IHC analysis of RBP1 using anti-RBP1 antibody (A03820-4). RBP1 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBP1 Antibody (A03820-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-ihc-testing-6.jpgAnti-RBP1 Antibody Picoband® IHC analysis of RBP1 using anti-RBP1 antibody (A03820-4). RBP1 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBP1 Antibody (A03820-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-if-testing-7.jpgAnti-RBP1 Antibody Picoband® IF analysis of RBP1 using anti-RBP1 antibody (A03820-4) and anti-Tubulin Alpha antibody (M03989-3). RBP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBP1 Antibody (A03820-4) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03820-4-rbp1-primary-antibodies-fcm-testing-8.jpgAnti-RBP1 Antibody Picoband® Flow Cytometry analysis of U2OS cells using anti-RBP1 antibody (A03820-4). Overlay histogram showing U2OS cells stained with A03820-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBP1 Antibody (A03820-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-retreg1-picoband-trade-antibody-a32125-boster.html2026-03-17T05:14:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32125-retreg1-primary-antibodies-wb-testing-1.jpgAnti-RETREG1 Antibody Picoband® Western blot analysis of RETREG1 using anti-RETREG1 antibody (A32125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RETREG1 antigen affinity purified polyclonal antibody (Catalog # A32125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RETREG1 at approximately 55 kDa. The expected band size for RETREG1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32125-retreg1-primary-antibodies-if-testing-2.jpgAnti-RETREG1 Antibody Picoband® IF analysis of RETREG1 using anti-RETREG1 antibody (A32125) and anti-Tubulin Alpha antibody (M03989-3). RETREG1 was detected in immunocytochemical section of U87 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RETREG1 Antibody (A32125) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32125-retreg1-primary-antibodies-fcm-testing-3.jpgAnti-RETREG1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RETREG1 antibody (A32125). Overlay histogram showing HL-60 cells stained with A32125 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RETREG1 Antibody (A32125, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32125-retreg1-primary-antibodies-fcm-testing-4.jpgAnti-RETREG1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RETREG1 antibody (A32125). Overlay histogram showing HL-60 cells stained with A32125 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RETREG1 Antibody (A32125, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rexo2-picoband-trade-antibody-a10835-1-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10835-1-rexo2-primary-antibodies-wb-testing-1.jpgAnti-REXO2 Antibody Picoband® Western blot analysis of REXO2 using anti-REXO2 antibody (A10835-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REXO2 antigen affinity purified polyclonal antibody (Catalog # A10835-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REXO2 at approximately 27 kDa. The expected band size for REXO2 is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rfx3-picoband-trade-antibody-a07674-2-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07674-2-rfx3-primary-antibodies-wb-testing-1.jpgAnti-RFX3 Antibody Picoband® Western blot analysis of RFX3 using anti-RFX3 antibody (A07674-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX3 antigen affinity purified polyclonal antibody (Catalog # A07674-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFX3 at approximately 95 kDa. The expected band size for RFX3 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07674-2-rfx3-primary-antibodies-ihc-testing-4.jpgAnti-RFX3 Antibody Picoband®IHC analysis of RFX3 using anti-RFX3 antibody (A07674-2). RFX3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX3 Antibody (A07674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07674-2-rfx3-primary-antibodies-ihc-testing-2.jpgAnti-RFX3 Antibody Picoband® IHC analysis of RFX3 using anti-RFX3 antibody (A07674-2). RFX3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX3 Antibody (A07674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07674-2-rfx3-primary-antibodies-ihc-testing-3.jpgAnti-RFX3 Antibody Picoband® IHC analysis of RFX3 using anti-RFX3 antibody (A07674-2). RFX3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX3 Antibody (A07674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07674-2-rfx3-primary-antibodies-if-testing-4.jpgAnti-RFX3 Antibody Picoband® IF analysis of RFX3 using anti-RFX3 antibody (A07674-2) and anti-Tubulin Alpha antibody (M03989-3). RFX3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RFX3 Antibody (A07674-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07674-2-rfx3-primary-antibodies-if-testing-5.jpgAnti-RFX3 Antibody Picoband® IF analysis of RFX3 using anti-RFX3 antibody (A07674-2). RFX3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RFX3 Antibody (A07674-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07674-2-rfx3-primary-antibodies-fcm-testing-6.jpgAnti-RFX3 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-RFX3 antibody (A07674-2). Overlay histogram showing RT4 cells stained with A07674-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFX3 Antibody (A07674-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rai14-picoband-trade-antibody-a09059-1-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09059-1-rai14-primary-antibodies-wb-testing-1.jpgAnti-RAI14 Antibody Picoband® Western blot analysis of RAI14 using anti-RAI14 antibody (A09059-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAI14 antigen affinity purified polyclonal antibody (Catalog # A09059-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAI14 at approximately 120 kDa. The expected band size for RAI14 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09059-1-rai14-primary-antibodies-fcm-testing-2.jpgAnti-RAI14 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-RAI14 antibody (A09059-1). Overlay histogram showing PC-3 cells stained with A09059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAI14 Antibody (A09059-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09059-1-rai14-primary-antibodies-fcm-testing-3.jpgAnti-RAI14 Antibody Picoband® Flow Cytometry analysis of U2OS cells using anti-RAI14 antibody (A09059-1). Overlay histogram showing U2OS cells stained with A09059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAI14 Antibody (A09059-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-foxp3-picoband-trade-antibody-a00011-3-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00011-3-foxp3-primary-antibodies-wb-testing-1.jpgAnti-FOXP3 Antibody Picoband® Western blot analysis of FOXP3 using anti-FOXP3 antibody (A00011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP3 antigen affinity purified polyclonal antibody (Catalog # A00011-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP3 at approximately 47 kDa. The expected band size for FOXP3 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-vcam1-picoband-trade-antibody-a01199-4-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-4-vcam1-primary-antibodies-wb-testing-1.jpgAnti-VCAM1 Antibody Picoband® Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human COLO320 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (Catalog # A01199-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 100 kDa. The expected band size for VCAM1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01199-4-vcam1-primary-antibodies-ihc-testing-1_1.jpgAnti-VCAM1 Antibody Picoband®IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199-4). VCAM1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 9.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-VCAM1 Antibody (A01199-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-gwl-mastl-picoband-trade-antibody-a04567-3-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04567-3-mastl-primary-antibodies-wb-testing-1.jpgAnti-GWL/MASTL Antibody Picoband® Western blot analysis of GWL/MASTL using anti-GWL/MASTL antibody (A04567-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GWL/MASTL antigen affinity purified polyclonal antibody (Catalog # A04567-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GWL/MASTL at approximately 110 kDa. The expected band size for GWL/MASTL is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04567-3-mastl-primary-antibodies-if-testing-2.jpgAnti-GWL/MASTL Antibody Picoband® IF analysis of GWL/MASTL using anti-GWL/MASTL antibody (A04567-3) and anti-Tubulin Alpha antibody (M03989-3). GWL/MASTL was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GWL/MASTL Antibody (A04567-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04567-3-mastl-primary-antibodies-fcm-testing-3.jpgAnti-GWL/MASTL Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-GWL/MASTL antibody (A04567-3). Overlay histogram showing MCF-7 cells stained with A04567-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GWL/MASTL Antibody (A04567-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-smoc1-picoband-trade-antibody-a07736-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07736-smoc1-primary-antibodies-wb-testing-1.jpgAnti-SMOC1 Antibody Picoband® Western blot analysis of SMOC1 using anti-SMOC1 antibody (A07736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOC1 antigen affinity purified polyclonal antibody (Catalog # A07736) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMOC1 at approximately 70 kDa. The expected band size for SMOC1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07736-smoc1-primary-antibodies-fcm-testing-2.jpgAnti-SMOC1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SMOC1 antibody (A07736). Overlay histogram showing HepG2 cells stained with A07736 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOC1 Antibody (A07736, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tab1-picoband-trade-antibody-a02847-1-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02847-1-tab1-primary-antibodies-wb-testing-1.jpgAnti-TAB1 Antibody Picoband® Western blot analysis of TAB1 using anti-TAB1 antibody (A02847-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB1 antigen affinity purified polyclonal antibody (Catalog # A02847-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB1 at approximately 55-65 kDa. The expected band size for TAB1 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-dbl-picoband-trade-antibody-a05775-1-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05775-1-dbl-primary-antibodies-wb-testing-1.jpgAnti-DBL Antibody Picoband® Western blot analysis of DBL using anti-DBL antibody (A05775-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBL antigen affinity purified polyclonal antibody (Catalog # A05775-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBL at approximately 125 kDa. The expected band size for DBL is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05775-1-dbl-primary-antibodies-fcm-testing-2.jpgAnti-DBL Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-DBL antibody (A05775-1). Overlay histogram showing MCF-7 cells stained with A05775-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBL Antibody (A05775-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05775-1-dbl-primary-antibodies-fcm-testing-3.jpgAnti-DBL Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-DBL antibody (A05775-1). Overlay histogram showing U251 cells stained with A05775-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBL Antibody (A05775-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ubxn8-picoband-trade-antibody-a13427-1-boster.html2026-03-17T05:14:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13427-1-ubxn8-primary-antibodies-wb-testing-1.jpgAnti-UBXN8 Antibody Picoband® Western blot analysis of UBXN8 using anti-UBXN8 antibody (A13427-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBXN8 antigen affinity purified polyclonal antibody (Catalog # A13427-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBXN8 at approximately 36 kDa. The expected band size for UBXN8 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13427-1-ubxn8-primary-antibodies-ihc-testing-2.jpgAnti-UBXN8 Antibody Picoband® IHC analysis of UBXN8 using anti-UBXN8 antibody (A13427-1). UBXN8 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBXN8 Antibody (A13427-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13427-1-ubxn8-primary-antibodies-fcm-testing-3.jpgAnti-UBXN8 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-UBXN8 antibody (A13427-1). Overlay histogram showing RT4 cells stained with A13427-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBXN8 Antibody (A13427-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13427-1-ubxn8-primary-antibodies-fcm-testing-4.jpgAnti-UBXN8 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-UBXN8 antibody (A13427-1). Overlay histogram showing U937 cells stained with A13427-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBXN8 Antibody (A13427-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-metabotropic-glutamate-receptor-5-grm5-picoband-trade-antibody-a01338-3-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01338-3-grm5-primary-antibodies-wb-testing-1.jpgAnti-Metabotropic Glutamate Receptor 5/GRM5 Antibody Picoband® Western blot analysis of Metabotropic Glutamate Receptor 5/GRM5 using anti-Metabotropic Glutamate Receptor 5/GRM5 antibody (A01338-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Metabotropic Glutamate Receptor 5/GRM5 antigen affinity purified polyclonal antibody (Catalog # A01338-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Metabotropic Glutamate Receptor 5/GRM5 at approximately 178 kDa. The expected band size for Metabotropic Glutamate Receptor 5/GRM5 is at 132 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-gcm1-picoband-trade-antibody-a04509-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04509-1-gcm1-primary-antibodies-wb-testing-1.jpgAnti-GCM1 Antibody Picoband® Western blot analysis of GCM1 using anti-GCM1 antibody (A04509-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCM1 antigen affinity purified polyclonal antibody (Catalog # A04509-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCM1 at approximately 49 kDa. The expected band size for GCM1 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04509-1-gcm1-primary-antibodies-if-testing-2.jpgAnti-GCM1 Antibody Picoband® IF analysis of GCM1 using anti-GCM1 antibody (A04509-1). GCM1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GCM1 Antibody (A04509-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tmem59-picoband-trade-antibody-a09364-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09364-1-tmem59-primary-antibodies-wb-testing-1.jpgAnti-TMEM59 Antibody Picoband® Western blot analysis of TMEM59 using anti-TMEM59 antibody (A09364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM59 antigen affinity purified polyclonal antibody (Catalog # A09364-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM59 at approximately 44 kDa. The expected band size for TMEM59 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09364-1-tmem59-primary-antibodies-fcm-testing-2.jpgAnti-TMEM59 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-TMEM59 antibody (A09364-1). Overlay histogram showing RT4 cells stained with A09364-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM59 Antibody (A09364-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rap1gap-picoband-trade-antibody-a03561-2-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03561-2-rap1gap-primary-antibodies-wb-testing-1.jpgAnti-RAP1GAP Antibody Picoband® Western blot analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GAP antigen affinity purified polyclonal antibody (Catalog # A03561-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1GAP at approximately 95 kDa. The expected band size for RAP1GAP is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03561-2-rap1gap-primary-antibodies-ihc-testing-2.jpgAnti-RAP1GAP Antibody Picoband® IHC analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). RAP1GAP was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAP1GAP Antibody (A03561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03561-2-rap1gap-primary-antibodies-ihc-testing-3.jpgAnti-RAP1GAP Antibody Picoband® IHC analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). RAP1GAP was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAP1GAP Antibody (A03561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03561-2-rap1gap-primary-antibodies-ihc-testing-4.jpgAnti-RAP1GAP Antibody Picoband® IHC analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). RAP1GAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAP1GAP Antibody (A03561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03561-2-rap1gap-primary-antibodies-ihc-testing-5.jpgAnti-RAP1GAP Antibody Picoband® IHC analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). RAP1GAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAP1GAP Antibody (A03561-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03561-2-rap1gap-primary-antibodies-if-testing-6.jpgAnti-RAP1GAP Antibody Picoband® IF analysis of RAP1GAP using anti-RAP1GAP antibody (A03561-2). RAP1GAP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAP1GAP Antibody (A03561-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03561-2-rap1gap-primary-antibodies-fcm-testing-7.jpgAnti-RAP1GAP Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RAP1GAP antibody (A03561-2). Overlay histogram showing MCF-7 cells stained with A03561-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAP1GAP Antibody (A03561-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rap1gds1-picoband-trade-antibody-a07861-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07861-1-rap1gds1-primary-antibodies-wb-testing-1.jpgAnti-RAP1GDS1 Antibody Picoband® Western blot analysis of RAP1GDS1 using anti-RAP1GDS1 antibody (A07861-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GDS1 antigen affinity purified polyclonal antibody (Catalog # A07861-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1GDS1 at approximately 66 kDa. The expected band size for RAP1GDS1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07861-1-rap1gds1-primary-antibodies-if-testing-2.jpgAnti-RAP1GDS1 Antibody Picoband® IF analysis of RAP1GDS1 using anti-RAP1GDS1 antibody (A07861-1). RAP1GDS1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAP1GDS1 Antibody (A07861-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07861-1-rap1gds1-primary-antibodies-fcm-testing-3.jpgAnti-RAP1GDS1 Antibody Picoband® Flow Cytometry analysis of U2OS cells using anti-RAP1GDS1 antibody (A07861-1). Overlay histogram showing U2OS cells stained with A07861-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAP1GDS1 Antibody (A07861-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rars-rars1-picoband-trade-antibody-a01418-2-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01418-2-rars1-primary-antibodies-wb-testing-1.jpgAnti-RARS/RARS1 Antibody Picoband® Western blot analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (A01418-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARS/RARS1 antigen affinity purified polyclonal antibody (Catalog # A01418-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RARS/RARS1 at approximately 70 kDa. The expected band size for RARS/RARS1 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01418-2-rars1-primary-antibodies-ihc-testing-2.jpgAnti-RARS/RARS1 Antibody Picoband® IHC analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (A01418-2). RARS/RARS1 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARS/RARS1 Antibody (A01418-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01418-2-rars1-primary-antibodies-ihc-testing-3.jpgAnti-RARS/RARS1 Antibody Picoband® IHC analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (A01418-2). RARS/RARS1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARS/RARS1 Antibody (A01418-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01418-2-rars1-primary-antibodies-ihc-testing-4.jpgAnti-RARS/RARS1 Antibody Picoband® IHC analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (A01418-2). RARS/RARS1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARS/RARS1 Antibody (A01418-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01418-2-rars1-primary-antibodies-if-testing-5.jpgAnti-RARS/RARS1 Antibody Picoband® IF analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (A01418-2) and anti-Tubulin Alpha antibody (M03989-3). RARS/RARS1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RARS/RARS1 Antibody (A01418-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01418-2-rars1-primary-antibodies-fcm-testing-6.jpgAnti-RARS/RARS1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RARS/RARS1 antibody (A01418-2). Overlay histogram showing MCF-7 cells stained with A01418-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RARS/RARS1 Antibody (A01418-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rasal2-picoband-trade-antibody-a07356-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07356-1-rasal2-primary-antibodies-wb-testing-1.jpgAnti-RASAL2 Antibody Picoband® Western blot analysis of RASAL2 using anti-RASAL2 antibody (A07356-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL2 antigen affinity purified polyclonal antibody (Catalog # A07356-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASAL2 at approximately 150 kDa. The expected band size for RASAL2 is at 129 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07356-1-rasal2-primary-antibodies-fcm-testing-2.jpgAnti-RASAL2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RASAL2 antibody (A07356-1). Overlay histogram showing HL-60 cells stained with A07356-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASAL2 Antibody (A07356-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-c18orf22-rbfa-picoband-trade-antibody-a13257-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-wb-testing-1.jpgAnti-C18orf22/RBFA Antibody Picoband® Western blot analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C18orf22/RBFA antigen affinity purified polyclonal antibody (Catalog # A13257-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C18orf22/RBFA at approximately 40 kDa. The expected band size for C18orf22/RBFA is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-ihc-testing-2.jpgAnti-C18orf22/RBFA Antibody Picoband® IHC analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). C18orf22/RBFA was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C18orf22/RBFA Antibody (A13257-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-ihc-testing-3.jpgAnti-C18orf22/RBFA Antibody Picoband® IHC analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). C18orf22/RBFA was detected in a paraffin-embedded section of human undifferentiated carcinoma of pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C18orf22/RBFA Antibody (A13257-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-ihc-testing-4.jpgAnti-C18orf22/RBFA Antibody Picoband® IHC analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). C18orf22/RBFA was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C18orf22/RBFA Antibody (A13257-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-ihc-testing-5.jpgAnti-C18orf22/RBFA Antibody Picoband® IHC analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). C18orf22/RBFA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C18orf22/RBFA Antibody (A13257-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-ihc-testing-6.jpgAnti-C18orf22/RBFA Antibody Picoband® IHC analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). C18orf22/RBFA was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C18orf22/RBFA Antibody (A13257-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-ihc-testing-7.jpgAnti-C18orf22/RBFA Antibody Picoband® IHC analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). C18orf22/RBFA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C18orf22/RBFA Antibody (A13257-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-if-testing-8.jpgAnti-C18orf22/RBFA Antibody Picoband® IF analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). C18orf22/RBFA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C18orf22/RBFA Antibody (A13257-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13257-1-rbfa-primary-antibodies-fcm-testing-9.jpgAnti-C18orf22/RBFA Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-C18orf22/RBFA antibody (A13257-1). Overlay histogram showing MCF-7 cells stained with A13257-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C18orf22/RBFA Antibody (A13257-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ranbp2-picoband-trade-antibody-a00981-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-wb-testing-1.jpgAnti-RANBP2 Antibody Picoband® Western blot analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP2 antigen affinity purified polyclonal antibody (Catalog # A00981-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANBP2 at approximately 358 kDa. The expected band size for RANBP2 is at 358 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-ihc-testing-2.jpgAnti-RANBP2 Antibody Picoband® IHC analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). RANBP2 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RANBP2 Antibody (A00981-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-ihc-testing-3.jpgAnti-RANBP2 Antibody Picoband® IHC analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). RANBP2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RANBP2 Antibody (A00981-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-ihc-testing-4.jpgAnti-RANBP2 Antibody Picoband® IHC analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). RANBP2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RANBP2 Antibody (A00981-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-ihc-testing-5.jpgAnti-RANBP2 Antibody Picoband® IHC analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). RANBP2 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RANBP2 Antibody (A00981-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-ihc-testing-6.jpgAnti-RANBP2 Antibody Picoband® IHC analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). RANBP2 was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RANBP2 Antibody (A00981-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-ihc-testing-7.jpgAnti-RANBP2 Antibody Picoband® IHC analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). RANBP2 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RANBP2 Antibody (A00981-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-if-testing-8.jpgAnti-RANBP2 Antibody Picoband® IF analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). RANBP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RANBP2 Antibody (A00981-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00981-1-ranbp2-primary-antibodies-fcm-testing-9_1.jpgAnti-RANBP2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RANBP2 antibody (A00981-1). Overlay histogram showing MCF-7 cells stained with A00981-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RANBP2 Antibody (A00981-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rasgrp1-picoband-trade-antibody-a03004-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03004-1-rasgrp1-primary-antibodies-wb-testing-1.jpgAnti-RASGRP1 Antibody Picoband® Western blot analysis of RASGRP1 using anti-RASGRP1 antibody (A03004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASGRP1 antigen affinity purified polyclonal antibody (Catalog # A03004-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASGRP1 at approximately 100 kDa. The expected band size for RASGRP1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03004-1-rasgrp1-primary-antibodies-fcm-testing-2.jpgAnti-RASGRP1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RASGRP1 antibody (A03004-1). Overlay histogram showing MCF-7 cells stained with A03004-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASGRP1 Antibody (A03004-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03004-1-rasgrp1-primary-antibodies-fcm-testing-3.jpgAnti-RASGRP1 Antibody Picoband® Flow Cytometry analysis of U2OS cells using anti-RASGRP1 antibody (A03004-1). Overlay histogram showing U2OS cells stained with A03004-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASGRP1 Antibody (A03004-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-irbp-rbp3-picoband-trade-antibody-a02913-1-boster.html2026-03-17T05:14:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02913-1-rbp3-primary-antibodies-wb-testing-1.jpgAnti-IRBP/RBP3 Antibody Picoband® Western blot analysis of IRBP/RBP3 using anti-IRBP/RBP3 antibody (A02913-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRBP/RBP3 antigen affinity purified polyclonal antibody (Catalog # A02913-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRBP/RBP3 at approximately 135 kDa. The expected band size for IRBP/RBP3 is at 135 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02913-1-rbp3-primary-antibodies-fcm-testing-2.jpgAnti-IRBP/RBP3 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-IRBP/RBP3 antibody (A02913-1). Overlay histogram showing MCF-7 cells stained with A02913-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IRBP/RBP3 Antibody (A02913-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sntg2-picoband-trade-antibody-a11619-2-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11619-2-sntg2-primary-antibodies-wb-testing-1.jpgAnti-SNTG2 Antibody Picoband® Western blot analysis of SNTG2 using anti-SNTG2 antibody (A11619-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNTG2 antigen affinity purified polyclonal antibody (Catalog # A11619-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNTG2 at approximately 60 kDa. The expected band size for SNTG2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11619-2-sntg2-primary-antibodies-fcm-testing-2.jpgAnti-SNTG2 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-SNTG2 antibody (A11619-2). Overlay histogram showing Daudi cells stained with A11619-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SNTG2 Antibody (A11619-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pon2-picoband-trade-antibody-a02027-2-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02027-2-pon2-primary-antibodies-wb-testing-1_1.jpgAnti-PON2 Antibody Picoband®Western blot analysis of PON2 using anti-PON2 antibody (A02027-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON2 antigen affinity purified polyclonal antibody (A02027-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PON2 at approximately 39, 40 kDa. The expected band size for PON2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02027-2-pon2-primary-antibodies-fcm-testing-2.jpgAnti-PON2 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-PON2 antibody (A02027-2). Overlay histogram showing RT4 cells stained with A02027-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PON2 Antibody (A02027-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-raver1-picoband-trade-antibody-a09359-2-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09359-2-raver1-primary-antibodies-wb-testing-1.jpgAnti-RAVER1 Antibody Picoband® Western blot analysis of RAVER1 using anti-RAVER1 antibody (A09359-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAVER1 antigen affinity purified polyclonal antibody (Catalog # A09359-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAVER1 at approximately 72 kDa. The expected band size for RAVER1 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09359-2-raver1-primary-antibodies-fcm-testing-2.jpgAnti-RAVER1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RAVER1 antibody (A09359-2). Overlay histogram showing HepG2 cells stained with A09359-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAVER1 Antibody (A09359-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rbm15b-picoband-trade-antibody-a11484-1-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-wb-testing-1.jpgAnti-RBM15B Antibody Picoband® Western blot analysis of RBM15B using anti-RBM15B antibody (A11484-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM15B antigen affinity purified polyclonal antibody (Catalog # A11484-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM15B at approximately 110 kDa. The expected band size for RBM15B is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-2.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-3.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-4.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-5.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-6.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-7.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-8.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-ihc-testing-9.jpgAnti-RBM15B Antibody Picoband® IHC analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human undifferentiated carcinoma of pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-if-testing-10.jpgAnti-RBM15B Antibody Picoband® IF analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-if-testing-11.jpgAnti-RBM15B Antibody Picoband® IF analysis of RBM15B using anti-RBM15B antibody (A11484-1). RBM15B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBM15B Antibody (A11484-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11484-1-rbm15b-primary-antibodies-fcm-testing-12.jpgAnti-RBM15B Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-RBM15B antibody (A11484-1). Overlay histogram showing Daudi cells stained with A11484-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15B Antibody (A11484-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rbm22-picoband-trade-antibody-a10311-1-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-wb-testing-1.jpgAnti-RBM22 Antibody Picoband® Western blot analysis of RBM22 using anti-RBM22 antibody (A10311-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM22 antigen affinity purified polyclonal antibody (Catalog # A10311-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM22 at approximately 47 kDa. The expected band size for RBM22 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-2.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-3.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-4.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-5.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-6.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-7.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-8.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-9.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-10.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-11.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-ihc-testing-12.jpgAnti-RBM22 Antibody Picoband® IHC analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-if-testing-13.jpgAnti-RBM22 Antibody Picoband® IF analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-if-testing-14.jpgAnti-RBM22 Antibody Picoband® IF analysis of RBM22 using anti-RBM22 antibody (A10311-1). RBM22 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBM22 Antibody (A10311-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10311-1-rbm22-primary-antibodies-fcm-testing-15.jpgAnti-RBM22 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-RBM22 antibody (A10311-1). Overlay histogram showing Daudi cells stained with A10311-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM22 Antibody (A10311-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rimkla-picoband-trade-antibody-a11921-2-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11921-2-rimkla-primary-antibodies-wb-testing-1.jpgAnti-RIMKLA Antibody Picoband® Western blot analysis of RIMKLA using anti-RIMKLA antibody (A11921-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMKLA antigen affinity purified polyclonal antibody (Catalog # A11921-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMKLA at approximately 43 kDa. The expected band size for RIMKLA is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tmem104-picoband-trade-antibody-a16095-1-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16095-1-tmem104-primary-antibodies-wb-testing-1.jpgAnti-TMEM104 Antibody Picoband® Western blot analysis of TMEM104 using anti-TMEM104 antibody (A16095-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM104 antigen affinity purified polyclonal antibody (Catalog # A16095-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM104 at approximately 56 kDa. The expected band size for TMEM104 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16095-1-tmem104-primary-antibodies-fcm-testing-2.jpgAnti-TMEM104 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-TMEM104 antibody (A16095-1). Overlay histogram showing HepG2 cells stained with A16095-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM104 Antibody (A16095-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnf44-picoband-trade-antibody-a16090-1-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16090-1-rnf44-primary-antibodies-wb-testing-1.jpgAnti-RNF44 Antibody Picoband® Western blot analysis of RNF44 using anti-RNF44 antibody (A16090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF44 antigen affinity purified polyclonal antibody (Catalog # A16090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF44 at approximately 48 kDa. The expected band size for RNF44 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16090-1-rnf44-primary-antibodies-fcm-testing-2.jpgAnti-RNF44 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RNF44 antibody (A16090-1). Overlay histogram showing HL-60 cells stained with A16090-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF44 Antibody (A16090-1, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnf212b-picoband-trade-antibody-a18911-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18911-rnf212b-primary-antibodies-wb-testing-1.jpgAnti-RNF212B Antibody Picoband® Western blot analysis of RNF212B using anti-RNF212B antibody (A18911). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse HBZY whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF212B antigen affinity purified polyclonal antibody (Catalog # A18911) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF212B at approximately 36 kDa. The expected band size for RNF212B is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18911-rnf212b-primary-antibodies-fcm-testing-2.jpgAnti-RNF212B Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RNF212B antibody (A18911). Overlay histogram showing MCF-7 cells stained with A18911 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF212B Antibody (A18911, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnmt-picoband-trade-antibody-a07752-1-boster.html2026-03-17T05:14:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07752-1-rnmt-primary-antibodies-wb-testing-1.jpgAnti-RNMT Antibody Picoband® Western blot analysis of RNMT using anti-RNMT antibody (A07752-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNMT antigen affinity purified polyclonal antibody (Catalog # A07752-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNMT at approximately 56 kDa. The expected band size for RNMT is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07752-1-rnmt-primary-antibodies-fcm-testing-2.jpgAnti-RNMT Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-RNMT antibody (A07752-1). Overlay histogram showing Daudi cells stained with A07752-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNMT Antibody (A07752-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rock2-picoband-trade-antibody-a01023-2-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01023-2-rock2-primary-antibodies-wb-testing-1.jpgAnti-ROCK2 Antibody Picoband® Western blot analysis of ROCK2 using anti-ROCK2 antibody (A01023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # A01023-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 180 kDa. The expected band size for ROCK2 is at 161 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01023-2-rock2-primary-antibodies-fcm-testing-2.jpgAnti-ROCK2 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-ROCK2 antibody (A01023-2). Overlay histogram showing A431 cells stained with A01023-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROCK2 Antibody (A01023-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rpain-picoband-trade-antibody-a11439-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11439-1-rpain-primary-antibodies-wb-testing-1.jpgAnti-RPAIN Antibody Picoband® Western blot analysis of RPAIN using anti-RPAIN antibody (A11439-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: mouse ovary tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPAIN antigen affinity purified polyclonal antibody (Catalog # A11439-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPAIN at approximately 35 kDa. The expected band size for RPAIN is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11439-1-rpain-primary-antibodies-fcm-testing-2.jpgAnti-RPAIN Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-RPAIN antibody (A11439-1). Overlay histogram showing RT4 cells stained with A11439-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPAIN Antibody (A11439-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ribophorin-i-rpn1-picoband-trade-antibody-a05063-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-1-rpn1-primary-antibodies-wb-testing-1.jpgAnti-Ribophorin I/RPN1 Antibody Picoband® Western blot analysis of Ribophorin I/RPN1 using anti-Ribophorin I/RPN1 antibody (A05063-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribophorin I/RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribophorin I/RPN1 at approximately 68 kDa. The expected band size for Ribophorin I/RPN1 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-p15rs-rprd1a-picoband-trade-antibody-a10062-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-wb-testing-1.jpgAnti-P15RS/RPRD1A Antibody Picoband® Western blot analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates. Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P15RS/RPRD1A antigen affinity purified polyclonal antibody (A10062-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P15RS/RPRD1A at approximately 36 kDa. The expected band size for P15RS/RPRD1A is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-ihc-testing-2.jpgAnti-P15RS/RPRD1A Antibody Picoband® IHC analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). P15RS/RPRD1A was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P15RS/RPRD1A Antibody (A10062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-ihc-testing-3.jpgAnti-P15RS/RPRD1A Antibody Picoband® IHC analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). P15RS/RPRD1A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P15RS/RPRD1A Antibody (A10062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-ihc-testing-4.jpgAnti-P15RS/RPRD1A Antibody Picoband® IHC analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). P15RS/RPRD1A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P15RS/RPRD1A Antibody (A10062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-ihc-testing-5.jpgAnti-P15RS/RPRD1A Antibody Picoband® IHC analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). P15RS/RPRD1A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P15RS/RPRD1A Antibody (A10062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-ihc-testing-6.jpgAnti-P15RS/RPRD1A Antibody Picoband® IHC analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). P15RS/RPRD1A was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P15RS/RPRD1A Antibody (A10062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-ihc-testing-7.jpgAnti-P15RS/RPRD1A Antibody Picoband® IHC analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). P15RS/RPRD1A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P15RS/RPRD1A Antibody (A10062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-ihc-testing-8.jpgAnti-P15RS/RPRD1A Antibody Picoband® IHC analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1). P15RS/RPRD1A was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P15RS/RPRD1A Antibody (A10062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-if-testing-9.jpgAnti-P15RS/RPRD1A Antibody Picoband® IF analysis of P15RS/RPRD1A using anti-P15RS/RPRD1A antibody (A10062-1) and anti-Tubulin Alpha antibody (M03989-3). P15RS/RPRD1A was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P15RS/RPRD1A Antibody (A10062-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10062-1-rprd1a-primary-antibodies-fcm-testing-10.jpgAnti-P15RS/RPRD1A Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-P15RS/RPRD1A antibody (A10062-1). Overlay histogram showing RT4 cells stained with A10062-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P15RS/RPRD1A Antibody (A10062-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ezh1-picoband-trade-antibody-a02494-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02494-1-ezh1-primary-antibodies-wb-testing-1.jpgAnti-EZH1 Antibody Picoband® Western blot analysis of EZH1 using anti-EZH1 antibody (A02494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH1 antigen affinity purified polyclonal antibody (Catalog # A02494-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EZH1 at approximately 100 kDa, 60 kDa. The expected band size for EZH1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02494-1-ezh1-primary-antibodies-wb-testing-2.jpgAnti-EZH1 Antibody Picoband® Western blot analysis of EZH1 using anti-EZH1 antibody (A02494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH1 antigen affinity purified polyclonal antibody (Catalog # A02494-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EZH1 at approximately 110 kDa, 60 kDa. The expected band size for EZH1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02494-1-ezh1-primary-antibodies-if-testing-3.jpgAnti-EZH1 Antibody Picoband® IF analysis of EZH1 using anti-EZH1 antibody (A02494-1) and anti-Tubulin Alpha antibody (M03989-3). EZH1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EZH1 Antibody (A02494-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02494-1-ezh1-primary-antibodies-fcm-testing-4.jpgAnti-EZH1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-EZH1 antibody (A02494-1). Overlay histogram showing RT4 cells stained with A02494-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EZH1 Antibody (A02494-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-swan-rbm12-picoband-trade-antibody-a13929-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13929-rbm12-primary-antibodies-wb-testing-1.jpgAnti-SWAN/RBM12 Antibody Picoband® Western blot analysis of SWAN/RBM12 using anti-SWAN/RBM12 antibody (A13929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SWAN/RBM12 antigen affinity purified polyclonal antibody (Catalog # A13929) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SWAN/RBM12 at approximately 97 kDa. The expected band size for SWAN/RBM12 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13929-rbm12-primary-antibodies-fcm-testing-2.jpgAnti-SWAN/RBM12 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-SWAN/RBM12 antibody (A13929). Overlay histogram showing RT4 cells stained with A13929 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SWAN/RBM12 Antibody (A13929, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rbm25-picoband-trade-antibody-a07494-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07494-1-rbm25-primary-antibodies-ihc-testing-4.jpgAnti-RBM25 Antibody Picoband®IHC analysis of RBM25 using anti-RBM25 antibody (A07494-1). RBM25 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM25 Antibody (A07494-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07494-1-rbm25-primary-antibodies-wb-testing-1_1.jpgAnti-RBM25 Antibody Picoband® Western blot analysis of RBM25 using anti-RBM25 antibody (A07494-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM25 antigen affinity purified polyclonal antibody (Catalog # A07494-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM25 at approximately 110-120 kDa. The expected band size for RBM25 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07494-1-rbm25-primary-antibodies-ihc-testing-2_1.jpgAnti-RBM25 Antibody Picoband® IHC analysis of RBM25 using anti-RBM25 antibody (A07494-1). RBM25 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM25 Antibody (A07494-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07494-1-rbm25-primary-antibodies-ihc-testing-3_1.jpgAnti-RBM25 Antibody Picoband® IHC analysis of RBM25 using anti-RBM25 antibody (A07494-1). RBM25 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM25 Antibody (A07494-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07494-1-rbm25-primary-antibodies-if-testing-4.jpgAnti-RBM25 Antibody Picoband® IF analysis of RBM25 using anti-RBM25 antibody (A07494-1) and anti-Tubulin Alpha antibody (M03989-3). RBM25 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBM25 Antibody (A07494-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07494-1-rbm25-primary-antibodies-fcm-testing-5_1.jpgAnti-RBM25 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-RBM25 antibody (A07494-1). Overlay histogram showing SiHa cells stained with A07494-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM25 Antibody (A07494-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-asparagine-synthetase-asns-picoband-trade-antibody-a03302-2-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03302-2-asns-primary-antibodies-wb-testing-1.jpgAnti-Asparagine synthetase/ASNS Antibody Picoband® Western blot analysis of Asparagine synthetase/ASNS using anti-Asparagine synthetase/ASNS antibody (A03302-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Asparagine synthetase/ASNS antigen affinity purified polyclonal antibody (Catalog # A03302-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Asparagine synthetase/ASNS at approximately 64 kDa. The expected band size for Asparagine synthetase/ASNS is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03302-2-asns-primary-antibodies-ihc-testing-2.jpgAnti-Asparagine synthetase/ASNS Antibody Picoband® IHC analysis of Asparagine synthetase/ASNS using anti-Asparagine synthetase/ASNS antibody (A03302-2). Asparagine synthetase/ASNS was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Asparagine synthetase/ASNS Antibody (A03302-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03302-2-asns-primary-antibodies-ihc-testing-3.jpgAnti-Asparagine synthetase/ASNS Antibody Picoband® IHC analysis of Asparagine synthetase/ASNS using anti-Asparagine synthetase/ASNS antibody (A03302-2). Asparagine synthetase/ASNS was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Asparagine synthetase/ASNS Antibody (A03302-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03302-2-asns-primary-antibodies-ihc-testing-4.jpgAnti-Asparagine synthetase/ASNS Antibody Picoband® IHC analysis of Asparagine synthetase/ASNS using anti-Asparagine synthetase/ASNS antibody (A03302-2). Asparagine synthetase/ASNS was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Asparagine synthetase/ASNS Antibody (A03302-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03302-2-asns-primary-antibodies-if-testing-5.jpgAnti-Asparagine synthetase/ASNS Antibody Picoband® IF analysis of Asparagine synthetase/ASNS using anti-Asparagine synthetase/ASNS antibody (A03302-2). Asparagine synthetase/ASNS was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Asparagine synthetase/ASNS Antibody (A03302-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03302-2-asns-primary-antibodies-fcm-testing-6.jpgAnti-Asparagine synthetase/ASNS Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Asparagine synthetase/ASNS antibody (A03302-2). Overlay histogram showing MCF-7 cells stained with A03302-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Asparagine synthetase/ASNS Antibody (A03302-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bok-picoband-trade-antibody-a05577-3-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05577-3-bok-primary-antibodies-wb-testing-1.jpgAnti-BOK Antibody Picoband® Western blot analysis of BOK using anti-BOK antibody (A05577-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse ovary tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BOK antigen affinity purified polyclonal antibody (Catalog # A05577-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BOK at approximately 27 kDa. The expected band size for BOK is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05577-3-bok-primary-antibodies-fcm-testing-2.jpgAnti-BOK Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-BOK antibody (A05577-3). Overlay histogram showing U251 cells stained with A05577-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BOK Antibody (A05577-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-uhrf1-picoband-trade-antibody-a01156-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01156-1-uhrf1-primary-antibodies-wb-testing-1.jpgAnti-UHRF1 Antibody Picoband® Western blot analysis of UHRF1 using anti-UHRF1 antibody (A01156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UHRF1 antigen affinity purified polyclonal antibody (Catalog # A01156-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UHRF1 at approximately 100 kDa. The expected band size for UHRF1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01156-1-uhrf1-primary-antibodies-if-testing-2.jpgAnti-UHRF1 Antibody Picoband® IF analysis of UHRF1 using anti-UHRF1 antibody (A01156-1) and anti-Tubulin Alpha antibody (M03989-3). UHRF1 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-UHRF1 Antibody (A01156-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01156-1-uhrf1-primary-antibodies-fcm-testing-3.jpgAnti-UHRF1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-UHRF1 antibody (A01156-1). Overlay histogram showing RT4 cells stained with A01156-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UHRF1 Antibody (A01156-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd39-entpd1-picoband-trade-antibody-a03196-4-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03196-4-entpd1-primary-antibodies-wb-testing-1.jpgAnti-CD39/Entpd1 Antibody Picoband® Western blot analysis of CD39/Entpd1 using anti-CD39/Entpd1 antibody (A03196-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD39/Entpd1 antigen affinity purified polyclonal antibody (Catalog # A03196-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD39/Entpd1 at approximately 100 kDa. The expected band size for CD39/Entpd1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03196-4-gr4.jpgAnti-CD39/Entpd1 Antibody Picoband®SSD treatment ameliorated RA rats. Representative images of hind paws from different groups and changes in foot circumference. Hematoxylin eosin staining and immunohistochemical staining (CD31, CD39, CD73, CCR6 and IL1R1) of knee joint synovial slices. *P < 0.05, **P < 0.01 vs Model group. Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)13288-4'>39296024</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03196-4-entpd1-primary-antibodies-ihc-testing-2.jpgAnti-CD39/Entpd1 Antibody Picoband® IHC analysis of CD39/Entpd1 using anti-CD39/Entpd1 antibody (A03196-4). CD39/Entpd1 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD39/Entpd1 Antibody (A03196-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03196-4-entpd1-primary-antibodies-fcm-testing-3.jpgAnti-CD39/Entpd1 Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-CD39/Entpd1 antibody (A03196-4). Overlay histogram showing ANA-1 cells stained with A03196-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD39/Entpd1 Antibody (A03196-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-atg12-picoband-trade-antibody-a00820-2-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00820-2-atg12-primary-antibodies-wb-testing-1.jpgAnti-ATG12 Antibody Picoband® Western blot analysis of ATG12 using anti-ATG12 antibody (A00820-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human COLO 320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG12 antigen affinity purified polyclonal antibody (Catalog # A00820-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG12 at approximately 48 kDa. The expected band size for ATG12 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00820-2-atg12-primary-antibodies-fcm-testing-2.jpgAnti-ATG12 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-ATG12 antibody (A00820-2). Overlay histogram showing RT4 cells stained with A00820-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG12 Antibody (A00820-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rbm11-picoband-trade-antibody-a13050-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13050-1-rbm11-primary-antibodies-wb-testing-1.jpgAnti-RBM11 Antibody Picoband® Western blot analysis of RBM11 using anti-RBM11 antibody (A13050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM11 antigen affinity purified polyclonal antibody (Catalog # A13050-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM11 at approximately 36 kDa. The expected band size for RBM11 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13050-1-rbm11-primary-antibodies-fcm-testing-2.jpgAnti-RBM11 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RBM11 antibody (A13050-1). Overlay histogram showing HEL cells stained with A13050-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM11 Antibody (A13050-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pirh2-rchy1-picoband-trade-antibody-a04533-3-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04533-3-rchy1-primary-antibodies-wb-testing-1.jpgAnti-Pirh2/RCHY1 Antibody Picoband® Western blot analysis of Pirh2/RCHY1 using anti-Pirh2/RCHY1 antibody (A04533-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pirh2/RCHY1 antigen affinity purified polyclonal antibody (Catalog # A04533-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pirh2/RCHY1 at approximately 21 kDa. The expected band size for Pirh2/RCHY1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04533-3-rchy1-primary-antibodies-fcm-testing-2.jpgAnti-Pirh2/RCHY1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Pirh2/RCHY1 antibody (A04533-3). Overlay histogram showing HepG2 cells stained with A04533-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Pirh2/RCHY1 Antibody (A04533-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-c-rel-rel-picoband-trade-antibody-a01880-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01880-1-rel-primary-antibodies-wb-testing-1.jpgAnti-c-Rel/REL Antibody Picoband® Western blot analysis of c-Rel/REL using anti-c-Rel/REL antibody (A01880-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified polyclonal antibody (Catalog # A01880-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01880-1-rel-primary-antibodies-if-testing-2.jpgAnti-c-Rel/REL Antibody Picoband® IF analysis of c-Rel/REL using anti-c-Rel/REL antibody (A01880-1). c-Rel/REL was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-c-Rel/REL Antibody (A01880-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01880-1-rel-primary-antibodies-fcm-testing-3.jpgAnti-c-Rel/REL Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-c-Rel/REL antibody (A01880-1). Overlay histogram showing U251 cells stained with A01880-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Rel/REL Antibody (A01880-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-retsat-picoband-trade-antibody-a11393-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11393-retsat-primary-antibodies-wb-testing-1.jpgAnti-RETSAT Antibody Picoband® Western blot analysis of RETSAT using anti-RETSAT antibody (A11393). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RETSAT antigen affinity purified polyclonal antibody (Catalog # A11393) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RETSAT at approximately 67 kDa. The expected band size for RETSAT is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11393-retsat-primary-antibodies-fcm-testing-2.jpgAnti-RETSAT Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RETSAT antibody (A11393). Overlay histogram showing HepG2 cells stained with A11393 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RETSAT Antibody (A11393, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rexo4-picoband-trade-antibody-a13732-1-boster.html2026-03-17T05:14:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13732-1-rexo4-primary-antibodies-wb-testing-1.jpgAnti-REXO4 Antibody Picoband® Western blot analysis of REXO4 using anti-REXO4 antibody (A13732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REXO4 antigen affinity purified polyclonal antibody (Catalog # A13732-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REXO4 at approximately 50 kDa. The expected band size for REXO4 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13732-1-rexo4-primary-antibodies-fcm-testing-2.jpgAnti-REXO4 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-REXO4 antibody (A13732-1). Overlay histogram showing HepG2 cells stained with A13732-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-REXO4 Antibody (A13732-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rfc2-picoband-trade-antibody-a07724-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07724-1-rfc2-primary-antibodies-wb-testing-1.jpgAnti-RFC2 Antibody Picoband® Western blot analysis of RFC2 using anti-RFC2 antibody (A07724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC2 antigen affinity purified polyclonal antibody (Catalog # A07724-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC2 at approximately 39 kDa. The expected band size for RFC2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07724-1-rfc2-primary-antibodies-if-testing-2.jpgAnti-RFC2 Antibody Picoband® IF analysis of RFC2 using anti-RFC2 antibody (A07724-1). RFC2 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RFC2 Antibody (A07724-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07724-1-rfc2-primary-antibodies-fcm-testing-3.jpgAnti-RFC2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-RFC2 antibody (A07724-1). Overlay histogram showing U251 cells stained with A07724-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFC2 Antibody (A07724-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rfc3-picoband-trade-antibody-a06442-2-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06442-2-rfc3-primary-antibodies-wb-testing-1.jpgAnti-RFC3 Antibody Picoband® Western blot analysis of RFC3 using anti-RFC3 antibody (A06442-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC3 antigen affinity purified polyclonal antibody (Catalog # A06442-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC3 at approximately 40 kDa. The expected band size for RFC3 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06442-2-rfc3-primary-antibodies-if-testing-2.jpgAnti-RFC3 Antibody Picoband® IF analysis of RFC3 using anti-RFC3 antibody (A06442-2) and anti-Tubulin Alpha antibody (M03989-3). RFC3 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RFC3 Antibody (A06442-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06442-2-rfc3-primary-antibodies-fcm-testing-3.jpgAnti-RFC3 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-RFC3 antibody (A06442-2). Overlay histogram showing U251 cells stained with A06442-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFC3 Antibody (A06442-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ribonuclease-h2-subunit-a-rnaseh2a-picoband-trade-antibody-a07386-2-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07386-2-rnaseh2a-primary-antibodies-wb-testing-1.jpgAnti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband® Western blot analysis of RNASEH2A using anti-RNASEH2A antibody (A07386-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASEH2A antigen affinity purified polyclonal antibody (Catalog # A07386-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASEH2A at approximately 33 kDa. The expected band size for RNASEH2A is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07386-2-rnaseh2a-primary-antibodies-fcm-testing-2.jpgAnti-Ribonuclease H2, subunit A/RNASEH2A Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-RNASEH2A antibody (A07386-2). Overlay histogram showing K562 cells stained with A07386-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNASEH2A Antibody (A07386-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnaseh2b-picoband-trade-antibody-a06660-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06660-1-rnaseh2b-primary-antibodies-wb-testing-1.jpgAnti-RNASEH2B Antibody Picoband® Western blot analysis of RNASEH2B using anti-RNASEH2B antibody (A06660-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASEH2B antigen affinity purified polyclonal antibody (Catalog # A06660-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASEH2B at approximately 35 kDa. The expected band size for RNASEH2B is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06660-1-rnaseh2b-primary-antibodies-if-testing-2.jpgAnti-RNASEH2B Antibody Picoband® IF analysis of RNASEH2B using anti-RNASEH2B antibody (A06660-1) and anti-Tubulin Alpha antibody (M03989-3). RNASEH2B was detected in immunocytochemical section of T-47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RNASEH2B Antibody (A06660-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06660-1-rnaseh2b-primary-antibodies-fcm-testing-3.jpgAnti-RNASEH2B Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RNASEH2B antibody (A06660-1). Overlay histogram showing HL-60 cells stained with A06660-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNASEH2B Antibody (A06660-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnf216-picoband-trade-antibody-a05841-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05841-1-rnf216-primary-antibodies-wb-testing-1.jpgAnti-RNF216 Antibody Picoband® Western blot analysis of RNF216 using anti-RNF216 antibody (A05841-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF216 antigen affinity purified polyclonal antibody (Catalog # A05841-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF216 at approximately 50 kDa. The expected band size for RNF216 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05841-1-rnf216-primary-antibodies-fcm-testing-2.jpgAnti-RNF216 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RNF216 antibody (A05841-1). Overlay histogram showing HepG2 cells stained with A05841-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF216 Antibody (A05841-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ap-b-rnpep-picoband-trade-antibody-a10683-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10683-1-rnpep-primary-antibodies-wb-testing-1.jpgAnti-AP-B/RNPEP Antibody Picoband® Western blot analysis of AP-B/RNPEP using anti-AP-B/RNPEP antibody (A10683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP-B/RNPEP antigen affinity purified polyclonal antibody (Catalog # A10683-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP-B/RNPEP at approximately 73 kDa. The expected band size for AP-B/RNPEP is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10683-1-rnpep-primary-antibodies-fcm-testing-2.jpgAnti-AP-B/RNPEP Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-AP-B/RNPEP antibody (A10683-1). Overlay histogram showing Hela cells stained with A10683-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AP-B/RNPEP Antibody (A10683-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10683-1-rnpep-primary-antibodies-fcm-testing-3.jpgAnti-AP-B/RNPEP Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-AP-B/RNPEP antibody (A10683-1). Overlay histogram showing U251 cells stained with A10683-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AP-B/RNPEP Antibody (A10683-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-plakophilin-2-pkp2-picoband-trade-antibody-a02146-1-boster.html2026-04-01T05:01:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02146-1-pkp2-primary-antibodies-wb-testing-1_1.jpgAnti-Plakophilin 2/PKP2 Antibody Picoband®Western blot analysis of Plakophilin 2/PKP2 using anti-Plakophilin 2/PKP2 antibody (A02146-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plakophilin 2/PKP2 antigen affinity purified polyclonal antibody (Catalog # A02146-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Plakophilin 2/PKP2 at approximately 100 kDa. The expected band size for Plakophilin 2/PKP2 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02146-1-pkp2-primary-antibodies-if-testing-1.jpgAnti-Plakophilin 2/PKP2 Antibody Picoband®IF analysis of Plakophilin 2/PKP2 using anti-Plakophilin 2/PKP2 antibody (A02146-1). Plakophilin 2/PKP2 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Plakophilin 2/PKP2 Antibody (A02146-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02146-1-pkp2-primary-antibodies-fcm-testing-1.jpgAnti-Plakophilin 2/PKP2 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-Plakophilin 2/PKP2 antibody (A02146-1). Overlay histogram showing HepG2 cells stained with A02146-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Plakophilin 2/PKP2 Antibody (A02146-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-hnrnp-e2-pcbp2-picoband-trade-antibody-a02425-3-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02425-3-pcbp2-primary-antibodies-wb-testing-1.jpgAnti-hnRNP E2/PCBP2 Antibody Picoband® Western blot analysis of hnRNP E2/PCBP2 using anti-hnRNP E2/PCBP2 antibody (A02425-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP E2/PCBP2 antigen affinity purified polyclonal antibody (Catalog # A02425-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP E2/PCBP2 at approximately 40 kDa. The expected band size for hnRNP E2/PCBP2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02425-3-pcbp2-primary-antibodies-if-testing-2.jpgAnti-hnRNP E2/PCBP2 Antibody Picoband® IF analysis of hnRNP E2/PCBP2 using anti-hnRNP E2/PCBP2 antibody (A02425-3) and anti-Tubulin Alpha antibody (M03989-3). hnRNP E2/PCBP2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-hnRNP E2/PCBP2 Antibody (A02425-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02425-3-pcbp2-primary-antibodies-fcm-testing-3.jpgAnti-hnRNP E2/PCBP2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-hnRNP E2/PCBP2 antibody (A02425-3). Overlay histogram showing Hela cells stained with A02425-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP E2/PCBP2 Antibody (A02425-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps5-picoband-trade-antibody-a04460-2-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-wb-testing-1.jpgAnti-RPS5 Antibody Picoband® Western blot analysis of RPS5 using anti-RPS5 antibody (A04460-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS5 antigen affinity purified polyclonal antibody (Catalog # A04460-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS5 at approximately 23 kDa. The expected band size for RPS5 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-ihc-testing-2.jpgAnti-RPS5 Antibody Picoband® IHC analysis of RPS5 using anti-RPS5 antibody (A04460-2). RPS5 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS5 Antibody (A04460-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-ihc-testing-3.jpgAnti-RPS5 Antibody Picoband® IHC analysis of RPS5 using anti-RPS5 antibody (A04460-2). RPS5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS5 Antibody (A04460-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-ihc-testing-4.jpgAnti-RPS5 Antibody Picoband® IHC analysis of RPS5 using anti-RPS5 antibody (A04460-2). RPS5 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS5 Antibody (A04460-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-ihc-testing-5.jpgAnti-RPS5 Antibody Picoband® IHC analysis of RPS5 using anti-RPS5 antibody (A04460-2). RPS5 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS5 Antibody (A04460-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-if-testing-6.jpgAnti-RPS5 Antibody Picoband® IF analysis of RPS5 using anti-RPS5 antibody (A04460-2). RPS5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS5 Antibody (A04460-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-if-testing-7.jpgAnti-RPS5 Antibody Picoband® IF analysis of RPS5 using anti-RPS5 antibody (A04460-2). RPS5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RPS5 Antibody (A04460-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-if-testing-8.jpgAnti-RPS5 Antibody Picoband® IF analysis of RPS5 using anti-RPS5 antibody (A04460-2). RPS5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RPS5 Antibody (A04460-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04460-2-rps5-primary-antibodies-fcm-testing-9.jpgAnti-RPS5 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-RPS5 antibody (A04460-2). Overlay histogram showing U251 cells stained with A04460-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS5 Antibody (A04460-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ranbp1-picoband-trade-antibody-a04000-3-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04000-3-ranbp1-primary-antibodies-wb-testing-1.jpgAnti-RANBP1 Antibody Picoband® Western blot analysis of RANBP1 using anti-RANBP1 antibody (A04000-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP1 antigen affinity purified polyclonal antibody (Catalog # A04000-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANBP1 at approximately 25 kDa. The expected band size for RANBP1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04000-3-ranbp1-primary-antibodies-if-testing-2.jpgAnti-RANBP1 Antibody Picoband® IF analysis of RANBP1 using anti-RANBP1 antibody (A04000-3). RANBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RANBP1 Antibody (A04000-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rassf2-picoband-trade-antibody-a04384-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04384-1-rassf2-primary-antibodies-wb-testing-1.jpgAnti-RASSF2 Antibody Picoband® Western blot analysis of RASSF2 using anti-RASSF2 antibody (A04384-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASSF2 antigen affinity purified polyclonal antibody (Catalog # A04384-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASSF2 at approximately 38 kDa. The expected band size for RASSF2 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04384-1-rassf2-primary-antibodies-if-testing-2.jpgAnti-RASSF2 Antibody Picoband® IF analysis of RASSF2 using anti-RASSF2 antibody (A04384-1) and anti-Tubulin Alpha antibody (M03989-3). RASSF2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RASSF2 Antibody (A04384-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04384-1-rassf2-primary-antibodies-fcm-testing-3.jpgAnti-RASSF2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RASSF2 antibody (A04384-1). Overlay histogram showing HL-60 cells stained with A04384-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASSF2 Antibody (A04384-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rbm14-picoband-trade-antibody-a04917-2-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04917-2-rbm14-primary-antibodies-wb-testing-1.jpgAnti-RBM14 Antibody Picoband® Western blot analysis of RBM14 using anti-RBM14 antibody (A04917-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM14 antigen affinity purified polyclonal antibody (Catalog # A04917-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM14 at approximately 75 kDa. The expected band size for RBM14 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04917-2-rbm14-primary-antibodies-if-testing-2.jpgAnti-RBM14 Antibody Picoband® IF analysis of RBM14 using anti-RBM14 antibody (A04917-2) and anti-Tubulin Alpha antibody (M03989-3). RBM14 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBM14 Antibody (A04917-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04917-2-rbm14-primary-antibodies-fcm-testing-3.jpgAnti-RBM14 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RBM14 antibody (A04917-2). Overlay histogram showing Hela cells stained with A04917-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM14 Antibody (A04917-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd276-picoband-trade-antibody-a02220-3-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02220-3-cd276-primary-antibodies-wb-testing-1.jpgAnti-CD276 Antibody Picoband® Western blot analysis of CD276 using anti-CD276 antibody (A02220-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD276 antigen affinity purified polyclonal antibody (Catalog # A02220-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD276 at approximately 90-100 kDa. The expected band size for CD276 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02220-3-cd276-primary-antibodies-ihc-testing-2.jpgAnti-CD276 Antibody Picoband® IHC analysis of CD276 using anti-CD276 antibody (A02220-3). CD276 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD276 Antibody (A02220-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02220-3-cd276-primary-antibodies-ihc-testing-3.jpgAnti-CD276 Antibody Picoband® IHC analysis of CD276 using anti-CD276 antibody (A02220-3). CD276 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD276 Antibody (A02220-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02220-3-cd276-primary-antibodies-ihc-testing-4.jpgAnti-CD276 Antibody Picoband® IHC analysis of CD276 using anti-CD276 antibody (A02220-3). CD276 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD276 Antibody (A02220-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02220-3-cd276-primary-antibodies-fcm-testing-5.jpgAnti-CD276 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-CD276 antibody (A02220-3). Overlay histogram showing PC-3 cells stained with A02220-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD276 Antibody (A02220-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/picokine-elisa-kits/human-b7-h2-icosl-picokine-elisa-kit-ek2217-boster.html2026-03-10T04:35:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2217.jpgHuman B7-H2/ICOSL ELISA Kit PicoKine®Human ICOSLG PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-epf-hsp10-picokine-elisa-kit-ek2218-boster.html2026-03-10T04:35:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2218.jpgHuman EPF/HSP10 ELISA Kit PicoKine®Human EPF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-icam4-picokine-elisa-kit-ek2219-boster.html2026-03-10T04:35:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2219.pngHuman ICAM4 ELISA Kit PicoKine®Human ICAM4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hs6st1-picokine-elisa-kit-ek2220-boster.html2026-03-10T04:35:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2220.jpgHuman HS6ST1 ELISA Kit PicoKine®Human HS6ST1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd11b-picokine-elisa-kit-ek2221-boster.html2026-03-10T04:35:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2221.pngHuman CD11b ELISA Kit PicoKine®Human CD11b PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd94-icosl-picokine-elisa-kit-ek2222-boster.html2026-03-10T04:35:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2222.jpgHuman CD94 ELISA Kit PicoKine®Human CD94 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd51-picokine-elisa-kit-ek2223-boster.html2026-03-10T04:35:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2223.jpgHuman CD51 ELISA Kit PicoKine®Human CD51 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-tmem50b-picoband-trade-antibody-a14199-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14199-1-tmem50b-primary-antibodies-wb-testing-1.jpgAnti-TMEM50B Antibody Picoband® Western blot analysis of TMEM50B using anti-TMEM50B antibody (A14199-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T47D whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM50B antigen affinity purified polyclonal antibody (Catalog # A14199-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM50B at approximately 23 kDa. The expected band size for TMEM50B is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14199-1-tmem50b-primary-antibodies-if-testing-2.jpgAnti-TMEM50B Antibody Picoband® IF analysis of TMEM50B using anti-TMEM50B antibody (A14199-1). TMEM50B was detected in an immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMEM50B Antibody (A14199-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14199-1-tmem50b-primary-antibodies-fcm-testing-3.pngAnti-TMEM50B Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-TMEM50B antibody (A14199-1). Overlay histogram showing U87 cells stained with A14199-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM50B Antibody (A14199-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-kif1a-picoband-trade-antibody-a03779-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03779-1-kif1a-primary-antibodies-wb-testing-1.jpgAnti-KIF1A Antibody Picoband® Western blot analysis of KIF1A using anti-KIF1A antibody (A03779-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brian tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF1A antigen affinity purified polyclonal antibody (Catalog # A03779-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF1A at approximately 191 kDa. The expected band size for KIF1A is at 191 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03779-1-kif1a-primary-antibodies-fcm-testing-2.pngAnti-KIF1A Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-KIF1A antibody (A03779-1). Overlay histogram showing 293T cells stained with A03779-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KIF1A Antibody (A03779-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03779-1-kif1a-primary-antibodies-fcm-testing-3.pngAnti-KIF1A Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-KIF1A antibody (A03779-1). Overlay histogram showing K562 cells stained with A03779-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KIF1A Antibody (A03779-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rag2-picoband-trade-antibody-a00352-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00352-rag2-primary-antibodies-wb-testing-1.jpgAnti-RAG2 Antibody Picoband® Western blot analysis of RAG2 using anti-RAG2 antibody (A00352). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAG2 antigen affinity purified polyclonal antibody (Catalog # A00352) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAG2 at approximately 55 kDa. The expected band size for RAG2 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00352-rag2-primary-antibodies-fcm-testing-2.pngAnti-RAG2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RAG2 antibody (A00352). Overlay histogram showing U20S cells stained with A00352 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAG2 Antibody (A00352, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rep15-picoband-trade-antibody-a14074-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14074-1-rep15-primary-antibodies-wb-testing-1.jpgAnti-REP15 Antibody Picoband® Western blot analysis of REP15 using anti-REP15 antibody (A14074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REP15 antigen affinity purified polyclonal antibody (Catalog # A14074-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REP15 at approximately 27 kDa. The expected band size for REP15 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14074-1-rep15-primary-antibodies-fcm-testing-2.pngAnti-REP15 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-REP15 antibody (A14074-1). Overlay histogram showing HepG2 cells stained with A14074-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-REP15 Antibody (A14074-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rftn1-picoband-trade-antibody-a11324-2-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11324-2-rftn1-primary-antibodies-wb-testing-1.jpgAnti-RFTN1 Antibody Picoband® Western blot analysis of RFTN1 using anti-RFTN1 antibody (A11324-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFTN1 antigen affinity purified polyclonal antibody (Catalog # A11324-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFTN1 at approximately 70 kDa. The expected band size for RFTN1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11324-2-rftn1-primary-antibodies-if-testing-2.jpgAnti-RFTN1 Antibody Picoband® IF analysis of RFTN1 using anti-RFTN1 antibody (A11324-2). RFTN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RFTN1 Antibody (A11324-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11324-2-rftn1-primary-antibodies-fcm-testing-3.pngAnti-RFTN1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-PITPNB antibody (A11324-2). Overlay histogram showing U20S cells stained with A11324-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITPNB Antibody (A11324-2, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rgl4-picoband-trade-antibody-a16655-1-boster.html2026-03-17T05:14:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16655-1-rgl4-primary-antibodies-wb-testing-1.jpgAnti-RGL4 Antibody Picoband® Western blot analysis of RGL4 using anti-RGL4 antibody (A16655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGL4 antigen affinity purified polyclonal antibody (Catalog # A16655-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGL4 at approximately 52 kDa. The expected band size for RGL4 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16655-1-rgl4-primary-antibodies-fcm-testing-2.pngAnti-RGL4 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-RGL4 antibody (A16655-1). Overlay histogram showing SiHa cells stained with A16655-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGL4 Antibody (A16655-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rgs9-picoband-trade-antibody-a04748-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04748-1-rgs9-primary-antibodies-wb-testing-1.jpgAnti-RGS9 Antibody Picoband® Western blot analysis of RGS9 using anti-RGS9 antibody (A04748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS9 antigen affinity purified polyclonal antibody (Catalog # A04748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS9 at approximately 77 kDa. The expected band size for RGS9 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04748-1-rgs9-primary-antibodies-if-testing-2.jpgAnti-RGS9 Antibody Picoband® IF analysis of RGS9 and Tubulin beta using anti-RGS9 antibody (A04748-1) and anti-Tubulin beta antibody (M05613-4). RGS9 and Tubulin beta was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RGS9 Antibody (A04748-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04748-1-rgs9-primary-antibodies-fcm-testing-3.pngAnti-RGS9 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RGS9 antibody (A04748-1). Overlay histogram showing HEL cells stained with A04748-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS9 Antibody (A04748-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rhbdf2-picoband-trade-antibody-a07693-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07693-1-rhbdf2-primary-antibodies-wb-testing-1.jpgAnti-RHBDF2 Antibody Picoband® Western blot analysis of RHBDF2 using anti-RHBDF2 antibody (A07693-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHBDF2 antigen affinity purified polyclonal antibody (Catalog # A07693-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHBDF2 at approximately 97 kDa. The expected band size for RHBDF2 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07693-1-rhbdf2-primary-antibodies-fcm-testing-2.pngAnti-RHBDF2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RHBDF2 antibody (A07693-1). Overlay histogram showing HepG2 cells stained with A07693-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RHBDF2 Antibody (A07693-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ptpip51-rmdn3-picoband-trade-antibody-a09297-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-wb-testing-1.jpgAnti-PTPIP51/RMDN3 Antibody Picoband® Western blot analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPIP51/RMDN3 antigen affinity purified polyclonal antibody (Catalog # A09297) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPIP51/RMDN3 at approximately 55 kDa. The expected band size for PTPIP51/RMDN3 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-ihc-testing-2.jpgAnti-PTPIP51/RMDN3 Antibody Picoband® IHC analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). PTPIP51/RMDN3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPIP51/RMDN3 Antibody (A09297) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-ihc-testing-3.jpgAnti-PTPIP51/RMDN3 Antibody Picoband® IHC analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). PTPIP51/RMDN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPIP51/RMDN3 Antibody (A09297) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-ihc-testing-4.jpgAnti-PTPIP51/RMDN3 Antibody Picoband® IHC analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). PTPIP51/RMDN3 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPIP51/RMDN3 Antibody (A09297) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-ihc-testing-5.jpgAnti-PTPIP51/RMDN3 Antibody Picoband® IHC analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). PTPIP51/RMDN3 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPIP51/RMDN3 Antibody (A09297) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-if-testing-6.jpgAnti-PTPIP51/RMDN3 Antibody Picoband® IF analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). PTPIP51/RMDN3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTPIP51/RMDN3 Antibody (A09297) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-if-testing-7.jpgAnti-PTPIP51/RMDN3 Antibody Picoband® IF analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (A09297). PTPIP51/RMDN3 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PTPIP51/RMDN3 Antibody (A09297) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09297-rmdn3-primary-antibodies-fcm-testing-8.pngAnti-PTPIP51/RMDN3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PTPIP51/RMDN3 antibody (A09297). Overlay histogram showing HepG2 cells stained with A09297 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTPIP51/RMDN3 Antibody (A09297, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnf34-picoband-trade-antibody-a08108-2-boster.html2026-04-03T05:00:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08108-2-rnf34-primary-antibodies-wb-testing-1.jpgAnti-RNF34 Antibody Picoband® Western blot analysis of RNF34 using anti-RNF34 antibody (A08108-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF34 antigen affinity purified polyclonal antibody (Catalog # A08108-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF34 at approximately 42 kDa. The expected band size for RNF34 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-ihc-testing-2.jpgAnti-RNF34 Antibody Picoband® IHC analysis of RNF34 using anti-RNF34 antibody (A07903-1). RNF34 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-ihc-testing-3.jpgAnti-RNF34 Antibody Picoband® IHC analysis of RNF34 using anti-RNF34 antibody (A07903-1). RNF34 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-ihc-testing-4.jpgAnti-RNF34 Antibody Picoband® IHC analysis of RNF34 using anti-RNF34 antibody (A07903-1). RNF34 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-ihc-testing-5.gifAnti-RNF34 Antibody Picoband® IHC analysis of RNF34 using anti-RNF34 antibody (A07903-1). RNF34 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-ihc-testing-6.jpgAnti-RNF34 Antibody Picoband® IHC analysis of RNF34 using anti-RNF34 antibody (A07903-1). RNF34 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-ihc-testing-7.jpgAnti-RNF34 Antibody Picoband® IHC analysis of RNF34 using anti-RNF34 antibody (A07903-1). RNF34 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-if-testing-8.jpgAnti-RNF34 Antibody Picoband® IF analysis of RNF34 and Tubulin beta using anti-RNF34 antibody (A07903-1) and anti-Tubulin beta antibody (M05613-4). RNF34 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RNF34 Antibody (A07903-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-fcm-testing-9.pngAnti-RNF34 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RNF34 antibody (A07903-1). Overlay histogram showing HEL cells stained with A07903-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF34 Antibody (A07903-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rnf34-primary-antibodies-fcm-testing-10.pngAnti-RNF34 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RNF34 antibody (A07903-1). Overlay histogram showing HL-60 cells stained with A07903-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF34 Antibody (A07903-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rimklb-picoband-trade-antibody-a14138-2-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14138-2-rimklb-primary-antibodies-wb-testing-1.jpgAnti-RIMKLB Antibody Picoband® Western blot analysis of RIMKLB using anti-RIMKLB antibody (A14138-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMKLB antigen affinity purified polyclonal antibody (Catalog # A14138-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMKLB at approximately 48 kDa. The expected band size for RIMKLB is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14138-2-rimklb-primary-antibodies-if-testing-2.jpgAnti-RIMKLB Antibody Picoband® IF analysis of RIMKLB and Tubulin beta using anti-RIMKLB antibody (A14138-2) and anti-Tubulin beta antibody (M05613-4). RIMKLB and Tubulin beta was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RIMKLB Antibody (A14138-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14138-2-rimklb-primary-antibodies-fcm-testing-3.pngAnti-RIMKLB Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RIMKLB antibody (A14138-2). Overlay histogram showing HEL cells stained with A14138-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIMKLB Antibody (A14138-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rfx1-picoband-trade-antibody-a04392-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04392-1-rfx1-primary-antibodies-wb-testing-1.jpgAnti-RFX1 Antibody Picoband® Western blot analysis of RFX1 using anti-RFX1 antibody (A04392-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX1 antigen affinity purified polyclonal antibody (Catalog # A04392-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFX1 at approximately 135 kDa. The expected band size for RFX1 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04392-1-rfx1-primary-antibodies-ihc-testing-2.jpgAnti-RFX1 Antibody Picoband® IHC analysis of RFX1 using anti-RFX1 antibody (A04392-1). RFX1 was detected in a paraffin-embedded section of Diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX1 Antibody (A04392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04392-1-rfx1-primary-antibodies-ihc-testing-3.jpgAnti-RFX1 Antibody Picoband® IHC analysis of RFX1 using anti-RFX1 antibody (A04392-1). RFX1 was detected in a paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX1 Antibody (A04392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04392-1-rfx1-primary-antibodies-ihc-testing-4.jpgAnti-RFX1 Antibody Picoband® IHC analysis of RFX1 using anti-RFX1 antibody (A04392-1). RFX1 was detected in a paraffin-embedded section of human Gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX1 Antibody (A04392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04392-1-rfx1-primary-antibodies-ihc-testing-5.jpgAnti-RFX1 Antibody Picoband® IHC analysis of RFX1 using anti-RFX1 antibody (A04392-1). RFX1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX1 Antibody (A04392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04392-1-rfx1-primary-antibodies-ihc-testing-6.jpgAnti-RFX1 Antibody Picoband® IHC analysis of RFX1 using anti-RFX1 antibody (A04392-1). RFX1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RFX1 Antibody (A04392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04392-1-rfx1-primary-antibodies-if-testing-7.jpgAnti-RFX1 Antibody Picoband® IF analysis of RFX1 using anti-RFX1 antibody (A04392-1). RFX1 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RFX1 Antibody (A04392-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rnf40-picoband-trade-antibody-a06979-2-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06979-2-rnf40-primary-antibodies-wb-testing-1.jpgAnti-RNF40 Antibody Picoband® Western blot analysis of RNF40 using anti-RNF40 antibody (A06979-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF40 antigen affinity purified polyclonal antibody (Catalog # A06979-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF40 at approximately 130 kDa. The expected band size for RNF40 is at 114 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06979-2-rnf40-primary-antibodies-ihc-testing-2.jpgAnti-RNF40 Antibody Picoband® IHC analysis of RNF40 using anti-RNF40 antibody (A06979-2). RNF40 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF40 Antibody (A06979-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06979-2-rnf40-primary-antibodies-ihc-testing-3.jpgAnti-RNF40 Antibody Picoband® IHC analysis of RNF40 using anti-RNF40 antibody (A06979-2). RNF40 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF40 Antibody (A06979-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06979-2-rnf40-primary-antibodies-ihc-testing-4.jpgAnti-RNF40 Antibody Picoband® IHC analysis of RNF40 using anti-RNF40 antibody (A06979-2). RNF40 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF40 Antibody (A06979-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06979-2-rnf40-primary-antibodies-if-testing-5.jpgAnti-RNF40 Antibody Picoband® IF analysis of RNF40 using anti-RNF401 antibody (A06979-2). RNF40 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RNF40 Antibody (A06979-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cd41-integrin-alpha-2b-itga2b-picoband-trade-antibody-a01102-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01102-1-itga2b-primary-antibodies-wb-testing-1.jpgAnti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband® Western blot analysis of CD41/Integrin Alpha 2B/ITGA2B using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD41/Integrin Alpha 2B/ITGA2B antigen affinity purified polyclonal antibody (Catalog # A01102-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD41/Integrin Alpha 2B/ITGA2B at approximately 125 kDa. The expected band size for CD41/Integrin Alpha 2B/ITGA2B is at 113 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01102-1-itga2b-primary-antibodies-ihc-testing-2.jpgAnti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband® IHC analysis of CD41/Integrin Alpha 2B/ITGA2B using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1). CD41/Integrin Alpha 2B/ITGA2B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD41/Integrin Alpha 2B/ITGA2B Antibody (A01102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01102-1-itga2b-primary-antibodies-ihc-testing-3.jpgAnti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband® IHC analysis of CD41/Integrin Alpha 2B/ITGA2B using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1). CD41/Integrin Alpha 2B/ITGA2B was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD41/Integrin Alpha 2B/ITGA2B Antibody (A01102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01102-1-itga2b-primary-antibodies-fcm-testing-4.pngAnti-CD41/Integrin Alpha 2B/ITGA2B Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-CD41/Integrin Alpha 2B/ITGA2B antibody (A01102-1). Overlay histogram showing HEL cells stained with A01102-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD41/Integrin Alpha 2B/ITGA2B Antibody (A01102-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rbmy1a1-picoband-trade-antibody-a06728-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06728-1-rbmy-primary-antibodies-wb-testing-1.jpgAnti-RBMY1A1 Antibody Picoband® Western blot analysis of RBMY1A1 using anti-RBMY1A1 antibody (A06728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMY1A1 antigen affinity purified polyclonal antibody (Catalog # A06728-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBMY1A1 at approximately 51 kDa. The expected band size for RBMY1A1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06728-1-rbmy-primary-antibodies-if-testing-2.jpgAnti-RBMY1A1 Antibody Picoband® IF analysis of RBMY1A1 and Tubulin beta using anti-RBMY1A1 antibody (A06728-1) and anti-Tubulin beta antibody (M05613-4). RBMY1A1 and Tubulin beta was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBMY1A1 Antibody (A06728-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rnf212-picoband-trade-antibody-a11605-2-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11605-2-rnf212-primary-antibodies-wb-testing-1.jpgAnti-RNF212 Antibody Picoband® Western blot analysis of RNF212 using anti-RNF212 antibody (A11605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF212 antigen affinity purified polyclonal antibody (Catalog # A11605-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF212 at approximately 22 kDa. The expected band size for RNF212 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11605-2-rnf212-primary-antibodies-fcm-testing-2.pngAnti-RNF212 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-RNF212 antibody (A11605-2). Overlay histogram showing SiHa cells stained with A11605-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF212 Antibody (A11605-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rom1-picoband-trade-antibody-a07035-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07035-1-rom1-primary-antibodies-wb-testing-1.jpgAnti-ROM1 Antibody Picoband® Western blot analysis of ROM1 using anti-ROM1 antibody (A07035-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROM1 antigen affinity purified polyclonal antibody (Catalog # A07035-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROM1 at approximately 37 kDa. The expected band size for ROM1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07035-1-rom1-primary-antibodies-fcm-testing-2.pngAnti-ROM1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-ROM1 antibody (A07035-1). Overlay histogram showing HEL cells stained with A07035-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ROM1 Antibody (A07035-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-mpo-picoband-trade-antibody-a00372-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00372-1-mpo-primary-antibodies-wb-testing-1.jpgAnti-MPO Antibody Picoband® Western blot analysis of MPO using anti-MPO antibody (A00372-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # A00372-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 55,80-90 kDa. The expected band size for MPO is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00372-1-mpo-primary-antibodies-ihc-testing-1.jpgAnti-MPO Antibody Picoband®IHC analysis of MPO using anti-MPO antibody (A00372-1). MPO was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody (A00372-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00372-1-mpo-primary-antibodies-fcm-testing-2.pngAnti-MPO Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-MPO antibody (A00372-1). Overlay histogram showing HL-60 cells stained with A00372-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody (A00372-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cyp27b1-picoband-trade-antibody-a01370-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01370-cyp27b1-primary-antibodies-wb-testing-1.jpgAnti-CYP27B1 Antibody Picoband® Western blot analysis of CYP27B1 using anti-CYP27B1 antibody (A01370). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP27B1 antigen affinity purified polyclonal antibody (Catalog # A01370) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP27B1 at approximately 57 kDa. The expected band size for CYP27B1 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sphk1-picoband-trade-antibody-a01390-2-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01390-2-sphk1-primary-antibodies-wb-testing-1.jpgAnti-SPHK1 Antibody Picoband® Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # A01390-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK1 at approximately 52 kDa. The expected band size for SPHK1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01390-2-sphk1-primary-antibodies-fcm-testing-2.pngAnti-SPHK1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SPHK1 antibody (A01390-2). Overlay histogram showing HepG2 cells stained with A01390-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (A01390-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ptn-picoband-trade-antibody-a01368-2-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01368-2-ptn-primary-antibodies-wb-testing-1.jpgAnti-PTN Antibody Picoband® Western blot analysis of PTN using anti-PTN antibody (A01368-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTN antigen affinity purified polyclonal antibody (Catalog # A01368-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTN at approximately 19 kDa. The expected band size for PTN is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01368-2-ptn-primary-antibodies-ihc-testing-2.jpgAnti-PTN Antibody Picoband® IHC analysis of PTN using anti-PTN antibody (A01368-2). PTN was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTN Antibody (A01368-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01368-2-ptn-primary-antibodies-ihc-testing-3.jpgAnti-PTN Antibody Picoband® IHC analysis of PTN using anti-PTN antibody (A01368-2). PTN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTN Antibody (A01368-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01368-2-ptn-primary-antibodies-ihc-testing-4.jpgAnti-PTN Antibody Picoband® IHC analysis of PTN using anti-PTN antibody (A01368-2). PTN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTN Antibody (A01368-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01368-2-ptn-primary-antibodies-fcm-testing-5.pngAnti-PTN Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-PTN antibody (A01368-2). Overlay histogram showing U20S cells stained with A01368-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PTN Antibody (A01368-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rngtt-picoband-trade-antibody-a09317-1-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-wb-testing-1.jpgAnti-RNGTT Antibody Picoband® Western blot analysis of RNGTT using anti-RNGTT antibody (A09317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNGTT antigen affinity purified polyclonal antibody (Catalog # A09317-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNGTT at approximately 69 kDa. The expected band size for RNGTT is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-ihc-testing-2.jpgAnti-RNGTT Antibody Picoband® IHC analysis of RNGTT using anti-RNGTT antibody (A09317-1). RNGTT was detected in a paraffin-embedded section of Diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNGTT Antibody (A09317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-ihc-testing-3.jpgAnti-RNGTT Antibody Picoband® IHC analysis of RNGTT using anti-RNGTT antibody (A09317-1). RNGTT was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNGTT Antibody (A09317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-ihc-testing-4.jpgAnti-RNGTT Antibody Picoband® IHC analysis of RNGTT using anti-RNGTT antibody (A09317-1). RNGTT was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNGTT Antibody (A09317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-ihc-testing-5.jpgAnti-RNGTT Antibody Picoband® IHC analysis of RNGTT using anti-RNGTT antibody (A09317-1). RNGTT was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNGTT Antibody (A09317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-ihc-testing-6.jpgAnti-RNGTT Antibody Picoband® IHC analysis of RNGTT using anti-RNGTT antibody (A09317-1). RNGTT was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNGTT Antibody (A09317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-if-testing-7.jpgAnti-RNGTT Antibody Picoband® IF analysis of RNGTT and Tubulin beta using anti-RNGTT antibody (A09317-1) and anti-Tubulin beta antibody (M05613-4). RNGTT and Tubulin beta was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RNGTT Antibody (A09317-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-if-testing-8.jpgAnti-RNGTT Antibody Picoband® IF analysis of RNGTT using anti-RNGTT antibody (A09317-1). RNGTT was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RNGTT Antibody (A09317-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09317-1-rngtt-primary-antibodies-fcm-testing-9.pngAnti-RNGTT Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-RNGTT antibody (A09317-1). Overlay histogram showing SiHa cells stained with A09317-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNGTT Antibody (A09317-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rpgrip1l-picoband-trade-antibody-a04178-2-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04178-2-rpgrip1l-primary-antibodies-wb-testing-1.jpgAnti-RPGRIP1L Antibody Picoband® Western blot analysis of RPGRIP1L using anti-RPGRIP1L antibody (A04178-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGRIP1L antigen affinity purified polyclonal antibody (Catalog # A04178-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGRIP1L at approximately 151 kDa. The expected band size for RPGRIP1L is at 151 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04178-2-rpgrip1l-primary-antibodies-fcm-testing-2.pngAnti-RPGRIP1L Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RPGRIP1L antibody (A04178-2). Overlay histogram showing HepG2 cells stained with A04178-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPGRIP1L Antibody (A04178-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ccdc88a-picoband-trade-antibody-a03282-3-boster.html2026-03-17T05:14:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03282-3-ccdc88a-primary-antibodies-wb-testing-1.jpgAnti-CCDC88A Antibody Picoband® Western blot analysis of CCDC88A using anti-CCDC88A antibody (A03282-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC88A antigen affinity purified polyclonal antibody (Catalog # A03282-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC88A at approximately 240 kDa. The expected band size for CCDC88A is at 216 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03282-3-ccdc88a-primary-antibodies-fcm-testing-2.pngAnti-CCDC88A Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-CCDC88A antibody (A03282-3). Overlay histogram showing HepG2 cells stained with A03282-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCDC88A Antibody (A03282-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rpn1-picoband-trade-antibody-a05063-2-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-2-rpn1-primary-antibodies-wb-testing-1.jpgAnti-RPN1 Antibody Picoband® Western blot analysis of RPN1 using anti-RPN1 antibody (A05063-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN1 at approximately 69 kDa. The expected band size for RPN1 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-2-rpn1-primary-antibodies-ihc-testing-2.jpgAnti-RPN1 Antibody Picoband® IHC analysis of RPN1 using anti-RPN1 antibody (A05063-2). RPN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPN1 Antibody (A05063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-2-rpn1-primary-antibodies-ihc-testing-3.jpgAnti-RPN1 Antibody Picoband® IHC analysis of RPN1 using anti-RPN1 antibody (A05063-2). RPN1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPN1 Antibody (A05063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-2-rpn1-primary-antibodies-ihc-testing-4.jpgAnti-RPN1 Antibody Picoband® IHC analysis of RPN1 using anti-RPN1 antibody (A05063-2). RPN1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPN1 Antibody (A05063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-2-rpn1-primary-antibodies-ihc-testing-5.jpgAnti-RPN1 Antibody Picoband® IHC analysis of RPN1 using anti-RPN1 antibody (A05063-2). RPN1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPN1 Antibody (A05063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-2-rpn1-primary-antibodies-ihc-testing-6.jpgAnti-RPN1 Antibody Picoband® IHC analysis of RPN1 using anti-RPN1 antibody (A05063-2). RPN1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPN1 Antibody (A05063-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05063-2-rpn1-primary-antibodies-fcm-testing-7.pngAnti-RPN1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RPN1 antibody (A05063-2). Overlay histogram showing HepG2 cells stained with A05063-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPN1 Antibody (A05063-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps4-x-y1-y2-picoband-trade-antibody-a09096-1-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09096-1-rps4x-primary-antibodies-wb-testing-1.jpgAnti-RPS4/X/Y1/Y2 Antibody Picoband® Western blot analysis of RPS4/X/Y1/Y2 using anti-RPS4/X/Y1/Y2 antibody (A09096-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human COLO 320 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS4/X/Y1/Y2 antigen affinity purified polyclonal antibody (Catalog # A09096-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS4/X/Y1/Y2 at approximately 30 kDa. The expected band size for RPS4/X/Y1/Y2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09096-1-rps4x-primary-antibodies-fcm-testing-2.pngAnti-RPS4/X/Y1/Y2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RPS4/X/Y1/Y2 antibody (A09096-1). Overlay histogram showing HepG2 cells stained with A09096-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS4/X/Y1/Y2 Antibody (A09096-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rrp1b-picoband-trade-antibody-a08159-1-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08159-1-rrp1b-primary-antibodies-wb-testing-1.jpgAnti-RRP1B Antibody Picoband® Western blot analysis of RRP1B using anti-RRP1B antibody (A08159-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP1B antigen affinity purified polyclonal antibody (Catalog # A08159-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP1B at approximately 95 kDa. The expected band size for RRP1B is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08159-1-rrp1b-primary-antibodies-if-testing-2.jpgAnti-RRP1B Antibody Picoband® IF analysis of RRP1B and Tubulin beta using anti-RRP1B antibody (A08159-1) and anti-Tubulin beta antibody (M05613-4). RRP1B and Tubulin beta was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RRP1B Antibody (A08159-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08159-1-rrp1b-primary-antibodies-fcm-testing-3.pngAnti-RRP1B Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-RRP1B antibody (A08159-1). Overlay histogram showing SiHa cells stained with A08159-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP1B Antibody (A08159-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rrp7a-picoband-trade-antibody-a15583-2-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15583-2-rrp7a-primary-antibodies-wb-testing-1.jpgAnti-RRP7A Antibody Picoband® Western blot analysis of RRP7A using anti-RRP7A antibody (A15583-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A341 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP7A antigen affinity purified polyclonal antibody (Catalog # A15583-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP7A at approximately 37 kDa. The expected band size for RRP7A is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15583-2-rrp7a-primary-antibodies-ihc-testing-2.jpgAnti-RRP7A Antibody Picoband® IHC analysis of RRP7A using anti-RRP7A antibody (A15583-2). RRP7A was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRP7A Antibody (A15583-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15583-2-rrp7a-primary-antibodies-if-testing-3.jpgAnti-RRP7A Antibody Picoband® IF analysis of RRP7A and Tubulin beta using anti-RRP7A antibody (A15583-2) and anti-Tubulin beta antibody (M05613-4). RRP7A and Tubulin beta was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RRP7A Antibody (A15583-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15583-2-rrp7a-primary-antibodies-fcm-testing-4.pngAnti-RRP7A Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-RRP7A antibody (A15583-2). Overlay histogram showing SiHa cells stained with A15583-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP7A Antibody (A15583-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rrp8-picoband-trade-antibody-a09950-2-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09950-2-rrp8-primary-antibodies-wb-testing-1.jpgAnti-RRP8 Antibody Picoband® Western blot analysis of RRP8 using anti-RRP8 antibody (A09950-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP8 antigen affinity purified polyclonal antibody (Catalog # A09950-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP8 at approximately 65 kDa. The expected band size for RRP8 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09950-2-rrp8-primary-antibodies-ihc-testing-1.jpgAnti-RRP8 Antibody Picoband®IHC analysis of RRP8 using anti-RRP8 antibody (A09950-2). RRP8 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRP8 Antibody (A09950-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09950-2-rrp8-primary-antibodies-if-testing-2.jpgAnti-RRP8 Antibody Picoband® IF analysis of RRP8 and Tubulin beta using anti-RRP8 antibody (A09950-2) and anti-Tubulin beta antibody (M05613-4). RRP8 and Tubulin beta was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RRP8 Antibody (A09950-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09950-2-rrp8-primary-antibodies-fcm-testing-3.pngAnti-RRP8 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RRP8 antibody (A09950-2). Overlay histogram showing Hela cells stained with A09950-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP8 Antibody (A09950-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-c20orf74-ralgapa2-picoband-trade-antibody-a10416-1-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10416-1-ralgapa2-primary-antibodies-wb-testing-1.jpgAnti-C20orf74/RALGAPA2 Antibody Picoband® Western blot analysis of C20orf74/RALGAPA2 using anti-C20orf74/RALGAPA2 antibody (A10416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C20orf74/RALGAPA2 antigen affinity purified polyclonal antibody (Catalog # A10416-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C20orf74/RALGAPA2 at approximately 250 kDa. The expected band size for C20orf74/RALGAPA2 is at 211 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10416-1-ralgapa2-primary-antibodies-fcm-testing-2.pngAnti-C20orf74/RALGAPA2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-C20orf74/RALGAPA2 antibody (A10416-1). Overlay histogram showing HepG2 cells stained with A10416-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C20orf74/RALGAPA2 Antibody (A10416-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-il-1-beta-il1b-picoband-trade-antibody-a00101-4-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-4-il1b-primary-antibodies-wb-testing-1.jpgAnti-IL-1 Beta/Il1b Antibody Picoband® Western blot analysis of IL-1 Beta/Il1b using anti-IL-1 Beta/Il1b antibody (A00101-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 Beta/Il1b antigen affinity purified polyclonal antibody (Catalog # A00101-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 Beta/Il1b at approximately 36 kDa. The expected band size for IL-1 Beta/Il1b is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00101-4-feb413914-fig-0009-m.jpgAnti-IL-1 Beta/Il1b Antibody Picoband®Effects of OPN and OVE on activation of NF-ĸB pathways in LPS-stimulated RAW264.7 cells. Cells were pretreated with OVE or OPN at different concentrations for 1 h. Expression levels of p-NF-ĸB p65, NF-ĸB p65, p-IĸBα, and IĸBα were detected after 24 h of LPS treatment. (A) OPN treatment. (B) OVE treatment. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ###P < 0.001, ####P < 0.0001, compared to the untreated control group; ****P < 0.0001, compared to the LPS control group. Index in PubMed under a CC BY license. PMID: <a href='https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/2211-5463.13914'>39455284</a> https://www.bosterbio.com/products/primary-antibodies/anti-rimbp2-picoband-trade-antibody-a15057-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15057-rimbp2-primary-antibodies-wb-testing-1.jpgAnti-RIMBP2 Antibody Picoband® Western blot analysis of RIMBP2 using anti-RIMBP2 antibody (A15057). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tisue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMBP2 antigen affinity purified polyclonal antibody (Catalog # A15057) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMBP2 at approximately 150 kDa. The expected band size for RIMBP2 is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15057-rimbp2-primary-antibodies-ihc-testing-2.jpgAnti-RIMBP2 Antibody Picoband® IHC analysis of RIMBP2 using anti-RIMBP2 antibody (A15057). RIMBP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RIMBP2 Antibody (A15057) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15057-rimbp2-primary-antibodies-ihc-testing-3.jpgAnti-RIMBP2 Antibody Picoband® IHC analysis of RIMBP2 using anti-RIMBP2 antibody (A15057). RIMBP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RIMBP2 Antibody (A15057) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15057-rimbp2-primary-antibodies-if-testing-4.jpgAnti-RIMBP2 Antibody Picoband® IF analysis of RIMBP2 using anti-RIMBP2 antibody (A15057). RIMBP2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RIMBP2 Antibody (A15057) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15057-rimbp2-primary-antibodies-if-testing-5.jpgAnti-RIMBP2 Antibody Picoband® IF analysis of RIMBP2 using anti-RIMBP2 antibody (A15057). RIMBP2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RIMBP2 Antibody (A15057) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15057-rimbp2-primary-antibodies-fcm-testing-6.pngAnti-RIMBP2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RIMBP2 antibody (A15057). Overlay histogram showing U20S cells stained with A15057 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIMBP2 Antibody (A15057, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rin1-picoband-trade-antibody-a04953-1-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04953-1-rin1-primary-antibodies-wb-testing-1.jpgAnti-RIN1 Antibody Picoband® Western blot analysis of RIN1 using anti-RIN1 antibody (A04953-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIN1 antigen affinity purified polyclonal antibody (Catalog # A04953-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIN1 at approximately 90 kDa. The expected band size for RIN1 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04953-1-rin1-primary-antibodies-fcm-testing-2.pngAnti-RIN1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RIN1 antibody (A04953-1). Overlay histogram showing HepG2 cells stained with A04953-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIN1 Antibody (A04953-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-riok3-picoband-trade-antibody-a09262-3-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09262-3-riok3-primary-antibodies-wb-testing-1.jpgAnti-RIOK3 Antibody Picoband® Western blot analysis of RIOK3 using anti-RIOK3 antibody (A09262-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK3 antigen affinity purified polyclonal antibody (Catalog # A09262-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIOK3 at approximately 65 kDa. The expected band size for RIOK3 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09262-3-riok3-primary-antibodies-fcm-testing-2.pngAnti-RIOK3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RIOK3 antibody (A09262-3). Overlay histogram showing HepG2 cells stained with A09262-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIOK3 Antibody (A09262-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-fam65b-ripor2-picoband-trade-antibody-a32101-1-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32101-1-ripor2-primary-antibodies-wb-testing-1.jpgAnti-FAM65B/RIPOR2 Antibody Picoband® Western blot analysis of FAM65B/RIPOR2 using anti-FAM65B/RIPOR2 antibody (A32101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM65B/RIPOR2 antigen affinity purified polyclonal antibody (Catalog # A32101-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM65B/RIPOR2 at approximately 120 kDa. The expected band size for FAM65B/RIPOR2 is at 119 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32101-1-ripor2-primary-antibodies-fcm-testing-2.pngAnti-FAM65B/RIPOR2 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-FAM65B/RIPOR2 antibody (A32101-1). Overlay histogram showing HEL cells stained with A32101-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM65B/RIPOR2 Antibody (A32101-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rlbp1-picoband-trade-antibody-a05421-1-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05421-1-rlbp1-primary-antibodies-wb-testing-1.jpgAnti-RLBP1 Antibody Picoband® Western blot analysis of RLBP1 using anti-RLBP1 antibody (A05421-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLBP1 antigen affinity purified polyclonal antibody (Catalog # A05421-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLBP1 at approximately 36 kDa. The expected band size for RLBP1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05421-1-rlbp1-primary-antibodies-fcm-testing-2.pngAnti-RLBP1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RLBP1 antibody (A05421-1). Overlay histogram showing HepG2 cells stained with A05421-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RLBP1 Antibody (A05421-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rit2-picoband-trade-antibody-a07833-1-boster.html2026-03-17T05:14:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07833-1-rit2-primary-antibodies-wb-testing-1.jpgAnti-RIT2 Antibody Picoband® Western blot analysis of RIT2 using anti-RIT2 antibody (A07833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SK-N-SH whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIT2 antigen affinity purified polyclonal antibody (Catalog # A07833-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIT2 at approximately 17,21 kDa. The expected band size for RIT2 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07833-1-rit2-primary-antibodies-fcm-testing-2.pngAnti-RIT2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RIT2 antibody (A07833-1). Overlay histogram showing U20S cells stained with A07833-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIT2 Antibody (A07833-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rln3-picoband-trade-antibody-a05495-2-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05495-2-rln3-primary-antibodies-wb-testing-1.jpgAnti-RLN3 Antibody Picoband® Western blot analysis of RLN3 using anti-RLN3 antibody (A05495-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse tesits tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLN3 antigen affinity purified polyclonal antibody (Catalog # A05495-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLN3 at approximately 18-19 kDa. The expected band size for RLN3 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05495-2-rln3-primary-antibodies-wb-testing-2.pngAnti-RLN3 Antibody Picoband®Western blot analysis of RLN3 using anti-RLN3 antibody (A05495-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions. Lane 1: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLN3 antigen affinity purified polyclonal antibody (A05495-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RLN3 at approximately 19 kDa. The expected band size for RLN3 is at 15 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05495-2-rln3-primary-antibodies-fcm-testing-2.pngAnti-RLN3 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-RLN3 antibody (A05495-2). Overlay histogram showing K562 cells stained with A05495-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RLN3 Antibody (A05495-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnase6-picoband-trade-antibody-a14127-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14127-rnase6-primary-antibodies-wb-testing-1.jpgAnti-RNASE6 Antibody Picoband® Western blot analysis of RNASE6 using anti-RNASE6 antibody (A14127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASE6 antigen affinity purified polyclonal antibody (Catalog # A14127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASE6 at approximately 17 kDa. The expected band size for RNASE6 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14127-rnase6-primary-antibodies-fcm-testing-2.pngAnti-RNASE6 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RNASE6 antibody (A14127). Overlay histogram showing HEL cells stained with A14127 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNASE6 Antibody (A14127, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rnaseh1-picoband-trade-antibody-a06342-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06342-1-rnaseh1-primary-antibodies-wb-testing-1.jpgAnti-RNASEH1 Antibody Picoband® Western blot analysis of RNASEH1 using anti-RNASEH1 antibody (A06342-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASEH1 antigen affinity purified polyclonal antibody (Catalog # A06342-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASEH1 at approximately 38 kDa. The expected band size for RNASEH1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06342-1-rnaseh1-primary-antibodies-if-testing-2.jpgAnti-RNASEH1 Antibody Picoband® IF analysis of RNASEH1 and Tubulin beta using anti-RNASEH1 antibody (A06342-1) and anti-Tubulin beta antibody (M05613-4). RNASEH1 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RNASEH1 Antibody (A06342-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06342-1-rnaseh1-primary-antibodies-fcm-testing-3.pngAnti-RNASEH1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RNASEH1 antibody (A06342-1). Overlay histogram showing HepG2 cells stained with A06342-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNASEH1 Antibody (A06342-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps14-picoband-trade-antibody-a03696-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-1-rps14-primary-antibodies-wb-testing-1.jpgAnti-RPS14 Antibody Picoband® Western blot analysis of RPS14 using anti-RPS14 antibody (A03696-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS14 antigen affinity purified polyclonal antibody (Catalog # A03696-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS14 at approximately 16 kDa. The expected band size for RPS14 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-1-rps14-primary-antibodies-ihc-testing-2.jpgAnti-RPS14 Antibody Picoband® IHC analysis of RPS14 using anti-RPS14 antibody (A03696-1). RPS14 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS14 Antibody (A03696-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-1-rps14-primary-antibodies-fcm-testing-3.pngAnti-RPS14 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RPS14 antibody (A03696-1). Overlay histogram showing HepG2 cells stained with A03696-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS14 Antibody (A03696-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps15a-picoband-trade-antibody-a09164-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09164-1-rps15a-primary-antibodies-wb-testing-1.jpgAnti-RPS15A Antibody Picoband® Western blot analysis of RPS15A using anti-RPS15A antibody (A09164-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS15A antigen affinity purified polyclonal antibody (Catalog # A09164-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS15A at approximately 15 kDa. The expected band size for RPS15A is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09164-1-rps15a-primary-antibodies-ihc-testing-2.jpgAnti-RPS15A Antibody Picoband® IHC analysis of RPS15A using anti-RPS15A antibody (A09164-1). RPS15A was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS15A Antibody (A09164-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09164-1-rps15a-primary-antibodies-ihc-testing-3.jpgAnti-RPS15A Antibody Picoband® IHC analysis of RPS15A using anti-RPS15A antibody (A09164-1). RPS15A was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS15A Antibody (A09164-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09164-1-rps15a-primary-antibodies-ihc-testing-4.jpgAnti-RPS15A Antibody Picoband® IHC analysis of RPS15A using anti-RPS15A antibody (A09164-1). RPS15A was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS15A Antibody (A09164-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09164-1-rps15a-primary-antibodies-if-testing-5.jpgAnti-RPS15A Antibody Picoband® IF analysis of RPS15A using anti-RPS15A antibody (A09164-1). RPS15A was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS15A Antibody (A09164-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09164-1-rps15a-primary-antibodies-if-testing-6.jpgAnti-RPS15A Antibody Picoband® IF analysis of RPS15A using anti-RPS15A antibody (A09164-1). RPS15A was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RPS15A Antibody (A09164-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09164-1-rps15a-primary-antibodies-fcm-testing-7.pngAnti-RPS15A Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RPS15A antibody (A09164-1). Overlay histogram showing HL-60 cells stained with A09164-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS15A Antibody (A09164-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps17-picoband-trade-antibody-a05831-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05831-1-rps17-primary-antibodies-wb-testing-1.jpgAnti-RPS17 Antibody Picoband® Western blot analysis of RPS17 using anti-RPS17 antibody (A05831-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS17 antigen affinity purified polyclonal antibody (Catalog # A05831-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS17 at approximately 18 kDa. The expected band size for RPS17 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05831-1-rps17-primary-antibodies-ihc-testing-1.jpgAnti-RPS17 Antibody Picoband®IHC analysis of RPS17 using anti-RPS17 antibody (A05831-1). RPS17 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS17 Antibody (A05831-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05831-1-rps17-primary-antibodies-if-testing-2.jpgAnti-RPS17 Antibody Picoband® IF analysis of RPS17 using anti-RPS17 antibody (A05831-1). RPS17 was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS17 Antibody (A05831-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05831-1-rps17-primary-antibodies-fcm-testing-3.pngAnti-RPS17 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-RPS17 antibody (A05831-1). Overlay histogram showing A431 cells stained with A05831-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS17 Antibody (A05831-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps25-picoband-trade-antibody-a09150-3-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09150-3-rps25-primary-antibodies-wb-testing-1.jpgAnti-RPS25 Antibody Picoband® Western blot analysis of RPS25 using anti-RPS25 antibody (A09150-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS25 antigen affinity purified polyclonal antibody (Catalog # A09150-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS25 at approximately 17 kDa. The expected band size for RPS25 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09150-3-rps25-primary-antibodies-ihc-testing-2.jpgAnti-RPS25 Antibody Picoband® IHC analysis of RPS25 using anti-RPS25 antibody (A09150-3). RPS25 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS25 Antibody (A09150-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09150-3-rps25-primary-antibodies-ihc-testing-3.jpgAnti-RPS25 Antibody Picoband® IHC analysis of RPS25 using anti-RPS25 antibody (A09150-3). RPS25 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS25 Antibody (A09150-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09150-3-rps25-primary-antibodies-ihc-testing-4.jpgAnti-RPS25 Antibody Picoband® IHC analysis of RPS25 using anti-RPS25 antibody (A09150-3). RPS25 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS25 Antibody (A09150-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09150-3-rps25-primary-antibodies-ihc-testing-5.jpgAnti-RPS25 Antibody Picoband® IHC analysis of RPS25 using anti-RPS25 antibody (A09150-3). RPS25 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS25 Antibody (A09150-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09150-3-rps25-primary-antibodies-fcm-testing-6.pngAnti-RPS25 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RPS25 antibody (A09150-3). Overlay histogram showing HL-60 cells stained with A09150-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS25 Antibody (A09150-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps28-picoband-trade-antibody-a10672-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10672-rps28-primary-antibodies-wb-testing-1_1.jpgAnti-RPS28 Antibody Picoband® Western blot analysis of RPS28 using anti-RPS28 antibody (A10672). Electrophoresis was performed on a 15% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH3T3 tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS28 antigen affinity purified polyclonal antibody (A10672) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPS28 at approximately 12 kDa. The expected band size for RPS28 is at 8 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10672-rps28-primary-antibodies-ihc-testing-2.jpgAnti-RPS28 Antibody Picoband® IHC analysis of RPS28 using anti-RPS28 antibody (A10672). RPS28 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS28 Antibody (A10672) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10672-rps28-primary-antibodies-ihc-testing-3.jpgAnti-RPS28 Antibody Picoband® IHC analysis of RPS28 using anti-RPS28 antibody (A10672). RPS28 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS28 Antibody (A10672) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0003) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10672-rps28-primary-antibodies-if-testing-4.jpgAnti-RPS28 Antibody Picoband® IF analysis of RPS28 using anti-RPS28 antibody (A10672) and anti-Tubulin Alpha antibody (M03989-3). RPS28 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS28 Antibody (A10672) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rrp1-picoband-trade-antibody-a06230-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06230-1-rrp1-primary-antibodies-wb-testing-1.jpgAnti-RRP1 Antibody Picoband® Western blot analysis of RRP1 using anti-RRP1 antibody (A06230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP1 antigen affinity purified polyclonal antibody (Catalog # A06230-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP1 at approximately 53 kDa. The expected band size for RRP1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06230-1-rrp1-primary-antibodies-ihc-testing-2.jpgAnti-RRP1 Antibody Picoband® IHC analysis of RRP1 using anti-RRP1 antibody (A06230-1). RRP1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRP1 Antibody (A06230-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06230-1-rrp1-primary-antibodies-ihc-testing-3.jpgAnti-RRP1 Antibody Picoband® IHC analysis of RRP1 using anti-RRP1 antibody (A06230-1). RRP1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RRP1 Antibody (A06230-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06230-1-rrp1-primary-antibodies-if-testing-4.jpgAnti-RRP1 Antibody Picoband® IF analysis of RRP1 and Tubulin beta using anti-RRP1 antibody (A06230-1) and anti-Tubulin beta antibody (M05613-4). RRP1 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RRP1 Antibody (A06230-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06230-1-rrp1-primary-antibodies-fcm-testing-5.pngAnti-RRP1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RRP1 antibody (A06230-1). Overlay histogram showing Hela cells stained with A06230-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP1 Antibody (A06230-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-odaph-picoband-trade-antibody-a32235-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32235-1-odaph-primary-antibodies-wb-testing-1.jpgAnti-ODAPH Antibody Picoband® Western blot analysis of ODAPH using anti-ODAPH antibody (A32235-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODAPH antigen affinity purified polyclonal antibody (Catalog # A32235-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODAPH at approximately 20 kDa. The expected band size for ODAPH is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-omp-picoband-trade-antibody-a01781-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01781-1-omp-primary-antibodies-wb-testing-1.jpgAnti-OMP Antibody Picoband® Western blot analysis of OMP using anti-OMP antibody (A01781-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMP antigen affinity purified polyclonal antibody (Catalog # A01781-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMP at approximately 17 kDa. The expected band size for OMP is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01781-1-omp-primary-antibodies-if-testing-2.jpgAnti-OMP Antibody Picoband® IF analysis of OMP using anti-OMP antibody (A01781-1). OMP was detected in an immunocytochemical section of CACO2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OMP Antibody (A01781-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01781-1-omp-primary-antibodies-fcm-testing-3.pngAnti-OMP Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-OMP antibody (A01781-1). Overlay histogram showing 293T cells stained with A01781-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OMP Antibody (A01781-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ogfr-picoband-trade-antibody-a06220-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06220-1-ogfr-primary-antibodies-wb-testing-1.jpgAnti-Ogfr Antibody Picoband® Western blot analysis of Ogfr using anti-Ogfr antibody (A06220-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ogfr antigen affinity purified polyclonal antibody (Catalog # A06220-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ogfr at approximately 110 kDa. The expected band size for Ogfr is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06220-1-ogfr-primary-antibodies-fcm-testing-2.pngAnti-Ogfr Antibody Picoband® Flow Cytometry analysis of ANA-1 cells using anti-Ogfr antibody (A06220-1). Overlay histogram showing ANA-1 cells stained with A06220-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ogfr Antibody (A06220-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmod2-picoband-trade-antibody-a12585-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12585-1-tmod2-primary-antibodies-wb-testing-1.jpgAnti-TMOD2 Antibody Picoband® Western blot analysis of TMOD2 using anti-TMOD2 antibody (A12585-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMOD2 antigen affinity purified polyclonal antibody (Catalog # A12585-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMOD2 at approximately 40 kDa. The expected band size for TMOD2 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12585-1-tmod2-primary-antibodies-if-testing-2.jpgAnti-TMOD2 Antibody Picoband® IF analysis of TMOD2 and Tubulin beta using anti-TMOD2 antibody (A12585-1) and anti-Tubulin beta antibody (M05613-4). TMOD2 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMOD2 Antibody (A12585-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12585-1-tmod2-primary-antibodies-fcm-testing-3.pngAnti-TMOD2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-TMOD2 antibody (A12585-1). Overlay histogram showing U20S cells stained with A12585-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMOD2 Antibody (A12585-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rock1-picoband-trade-antibody-a00722-5-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00722-5-rock1-primary-antibodies-wb-testing-1_1.jpgAnti-ROCK1 Antibody Picoband®Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-5). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified polyclonal antibody (A00722-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 158 kDa. The expected band size for ROCK1 is at 158 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00722-5-rock1-primary-antibodies-ihc-testing-1.jpgAnti-ROCK1 Antibody Picoband®IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5). ROCK1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ROCK1 Antibody (A00722-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00722-5-rock1-primary-antibodies-ihc-testing-2.jpgAnti-ROCK1 Antibody Picoband®IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5). ROCK1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ROCK1 Antibody (A00722-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00722-5-rock1-primary-antibodies-ihc-testing-3.jpgAnti-ROCK1 Antibody Picoband®IHC analysis of ROCK1 using anti-ROCK1 antibody (A00722-5). ROCK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ROCK1 Antibody (A00722-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00722-5-rock1-primary-antibodies-if-testing-1.jpgAnti-ROCK1 Antibody Picoband®IF analysis of ROCK1 using anti-ROCK1 antibody (A00722-5) and anti-Tubulin Alpha antibody (M03989-3). ROCK1 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ROCK1 Antibody (A00722-5) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rpn2-picoband-trade-antibody-a05006-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05006-1-rpn2-primary-antibodies-wb-testing-1.jpgAnti-RPN2 Antibody Picoband® Western blot analysis of RPN2 using anti-RPN2 antibody (A05006-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPN2 antigen affinity purified polyclonal antibody (Catalog # A05006-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPN2 at approximately 69 kDa. The expected band size for RPN2 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05006-1-rpn2-primary-antibodies-if-testing-2.jpgAnti-RPN2 Antibody Picoband® IF analysis of RPN2 using anti-RPN2 antibody (A05006-1). RPN2 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPN2 Antibody (A05006-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05006-1-rpn2-primary-antibodies-ihc-testing-1.jpgAnti-RPN2 Antibody Picoband®IHC analysis of RPN2 using anti-RPN2 antibody (A05006-1). RPN2 was detected in a paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPN2 Antibody (A05006-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05006-1-rpn2-primary-antibodies-fcm-testing-3.pngAnti-RPN2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RPN2 antibody (A05006-1). Overlay histogram showing HL-60 cells stained with A05006-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPN2 Antibody (A05006-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-occludin-ocln-picoband-trade-antibody-a01246-3-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01246-3-ocln-primary-antibodies-wb-testing-1.jpgAnti-Occludin/Ocln Antibody Picoband® Western blot analysis of Occludin/Ocln using anti-Occludin/Ocln antibody (A01246-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Occludin/Ocln antigen affinity purified polyclonal antibody (Catalog # A01246-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Occludin/Ocln at approximately 59 kDa. The expected band size for Occludin/Ocln is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-dr5-tnfrsf10b-picoband-trade-antibody-a00410-4-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-4-tnfrsf10b-primary-antibodies-wb-testing-1.jpgAnti-DR5/Tnfrsf10b Antibody Picoband® Western blot analysis of DR5/Tnfrsf10b using anti-DR5/Tnfrsf10b antibody (A00410-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR5/Tnfrsf10b antigen affinity purified polyclonal antibody (Catalog # A00410-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR5/Tnfrsf10b at approximately 48 kDa. The expected band size for DR5/Tnfrsf10b is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rpl11-picoband-trade-antibody-a02901-1-boster.html2026-03-17T05:14:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02901-1-rpl11-primary-antibodies-wb-testing-1.jpgAnti-RPL11 Antibody Picoband® Western blot analysis of RPL11 using anti-RPL11 antibody (A02901-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL11 antigen affinity purified polyclonal antibody (Catalog # A02901-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL11 at approximately 18 kDa. The expected band size for RPL11 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02901-1-rpl11-primary-antibodies-ihc-testing-2.jpgAnti-RPL11 Antibody Picoband® IHC analysis of RPL11 using anti-RPL11 antibody (A02901-1). RPL11 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL11 Antibody (A02901-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02901-1-rpl11-primary-antibodies-ihc-testing-3.jpgAnti-RPL11 Antibody Picoband® IHC analysis of RPL11 using anti-RPL11 antibody (A02901-1). RPL11 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL11 Antibody (A02901-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02901-1-rpl11-primary-antibodies-ihc-testing-4.jpgAnti-RPL11 Antibody Picoband® IHC analysis of RPL11 using anti-RPL11 antibody (A02901-1). RPL11 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL11 Antibody (A02901-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02901-1-rpl11-primary-antibodies-if-testing-5.jpgAnti-RPL11 Antibody Picoband® IF analysis of RPL11 using anti-RPL11 antibody (A02901-1). RPL11 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPL11 Antibody (A02901-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02901-1-rpl11-primary-antibodies-fcm-testing-6.pngAnti-RPL11 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-RPL11 antibody (A02901-1). Overlay histogram showing THP-1 cells stained with A02901-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL11 Antibody (A02901-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sccpdh-picoband-trade-antibody-a14281-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14281-1-sccpdh-primary-antibodies-wb-testing-1.jpgAnti-SCCPDH Antibody Picoband® Western blot analysis of SCCPDH using anti-SCCPDH antibody (A14281-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCCPDH antigen affinity purified polyclonal antibody (Catalog # A14281-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCCPDH at approximately 40 kDa. The expected band size for SCCPDH is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14281-1-sccpdh-primary-antibodies-if-testing-2.jpgAnti-SCCPDH Antibody Picoband® IF analysis of SCCPDH using anti-SCCPDH antibody (A14281-1). SCCPDH was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCCPDH Antibody (A14281-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-nup54-picoband-trade-antibody-a10830-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10830-1-nup54-primary-antibodies-wb-testing-1.jpgAnti-NUP54 Antibody Picoband® Western blot analysis of NUP54 using anti-NUP54 antibody (A10830-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP54 antigen affinity purified polyclonal antibody (Catalog # A10830-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP54 at approximately 56 kDa. The expected band size for NUP54 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-wfs1-picoband-trade-antibody-a01326-2-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01326-2-wfs1-primary-antibodies-wb-testing-1.jpgAnti-WFS1 Antibody Picoband® Western blot analysis of WFS1 using anti-WFS1 antibody (A01326-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WFS1 antigen affinity purified polyclonal antibody (Catalog # A01326-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WFS1 at approximately 100 kDa. The expected band size for WFS1 is at 100 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rtcb-picoband-trade-antibody-a05076-2-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-wb-testing-1_1.jpgAnti-RTCB Antibody Picoband® Western blot analysis of RTCB using anti-RTCB antibody (A05076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (Catalog # A05076-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-ihc-testing-2.jpgAnti-RTCB Antibody Picoband® IHC analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in a paraffin-embedded section of human parotid acinar cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-ihc-testing-3.jpgAnti-RTCB Antibody Picoband® IHC analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-ihc-testing-4.jpgAnti-RTCB Antibody Picoband® IHC analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-ihc-testing-5.jpgAnti-RTCB Antibody Picoband® IHC analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in a paraffin-embedded section of human skin squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-ihc-testing-6.jpgAnti-RTCB Antibody Picoband® IHC analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-ihc-testing-7.jpgAnti-RTCB Antibody Picoband® IHC analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-ihc-testing-8.jpgAnti-RTCB Antibody Picoband® IHC analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-if-testing-9.jpgAnti-RTCB Antibody Picoband® IF analysis of RTCB using anti-RTCB antibody (A05076-2). RTCB was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RTCB Antibody (A05076-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05076-2-rtcb-primary-antibodies-fcm-testing-10.jpgAnti-RTCB Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RTCB antibody (A05076-2). Overlay histogram showing Hela cells stained with A05076-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTCB Antibody (A05076-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-rtel1-picoband-trade-antibody-a01544-2-boster.html2026-04-03T05:00:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01544-2-rtel1-primary-antibodies-wb-testing-1.jpgAnti-RTEL1 Antibody Picoband® Western blot analysis of RTEL1 using anti-RTEL1 antibody (A01544-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTEL1 antigen affinity purified polyclonal antibody (Catalog # A01544-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTEL1 at approximately 160 kDa. The expected band size for RTEL1 is at 134 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01544-2-rtel1-primary-antibodies-fcm-testing-2.pngAnti-RTEL1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RTEL1 antibody (A01544-2). Overlay histogram showing HepG2 cells stained with A01544-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTEL1 Antibody (A01544-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rtf1-picoband-trade-antibody-a05784-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05784-1-rtf1-primary-antibodies-wb-testing-1.jpgAnti-RTF1 Antibody Picoband® Western blot analysis of RTF1 using anti-RTF1 antibody (A05784-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTF1 antigen affinity purified polyclonal antibody (Catalog # A05784-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTF1 at approximately 100 kDa. The expected band size for RTF1 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05784-1-rtf1-primary-antibodies-ihc-testing-4.jpgAnti-RTF1 Antibody Picoband®IHC analysis of RTF1 using anti-RTF1 antibody (A05784-1). RTF1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTF1 Antibody (A05784-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05784-1-rtf1-primary-antibodies-ihc-testing-2.jpgAnti-RTF1 Antibody Picoband® IHC analysis of RTF1 using anti-RTF1 antibody (A05784-1). RTF1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTF1 Antibody (A05784-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05784-1-rtf1-primary-antibodies-ihc-testing-3.jpgAnti-RTF1 Antibody Picoband® IHC analysis of RTF1 using anti-RTF1 antibody (A05784-1). RTF1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTF1 Antibody (A05784-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05784-1-rtf1-primary-antibodies-fcm-testing-4.pngAnti-RTF1 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-RTF1 antibody (A05784-1). Overlay histogram showing Daudi cells stained with A05784-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTF1 Antibody (A05784-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rtkn-picoband-trade-antibody-a07596-2-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07596-2-rtkn-primary-antibodies-wb-testing-1.jpgAnti-RTKN Antibody Picoband® Western blot analysis of RTKN using anti-RTKN antibody (A07596-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTKN antigen affinity purified polyclonal antibody (Catalog # A07596-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTKN at approximately 70 kDa. The expected band size for RTKN is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07596-2-rtkn-primary-antibodies-fcm-testing-2.pngAnti-RTKN Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-RTKN antibody (A07596-2). Overlay histogram showing CACO-2 cells stained with A07596-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTKN Antibody (A07596-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-nogo-receptor-ngr1-rtn4r-picoband-trade-antibody-a02250-2-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02250-2-rtn4r-primary-antibodies-wb-testing-1.jpgAnti-Nogo receptor/NgR1/RTN4R Antibody Picoband® Western blot analysis of Nogo Receptor/NgR1/RTN4R using anti-Nogo Receptor/NgR1/RTN4R antibody (A02250-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo Receptor/NgR1/RTN4R antigen affinity purified polyclonal antibody (Catalog # A02250-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo Receptor/NgR1/RTN4R at approximately 70 kDa. The expected band size for Nogo Receptor/NgR1/RTN4R is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rufy4-picoband-trade-antibody-a16707-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16707-1-rufy4-primary-antibodies-wb-testing-1.jpgAnti-RUFY4 Antibody Picoband® Western blot analysis of RUFY4 using anti-RUFY4 antibody (A16707-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY4 antigen affinity purified polyclonal antibody (Catalog # A16707-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY4 at approximately 64 kDa. The expected band size for RUFY4 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16707-1-rufy4-primary-antibodies-ihc-testing-2.jpgAnti-RUFY4 Antibody Picoband® IHC analysis of RUFY4 using anti-RUFY4 antibody (A16707-1). RUFY4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RUFY4 Antibody (A16707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16707-1-rufy4-primary-antibodies-fcm-testing-3.pngAnti-RUFY4 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RUFY4 antibody (A16707-1). Overlay histogram showing HEL cells stained with A16707-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUFY4 Antibody (A16707-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16707-1-rufy4-primary-antibodies-fcm-testing-4.pngAnti-RUFY4 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-RUFY4 antibody (A16707-1). Overlay histogram showing THP-1 cells stained with A16707-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUFY4 Antibody (A16707-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rundc1-picoband-trade-antibody-a14070-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14070-rundc1-primary-antibodies-wb-testing-1.jpgAnti-RUNDC1 Antibody Picoband® Western blot analysis of RUNDC1 using anti-RUNDC1 antibody (A14070). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNDC1 antigen affinity purified polyclonal antibody (Catalog # A14070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNDC1 at approximately 72 kDa. The expected band size for RUNDC1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14070-rundc1-primary-antibodies-fcm-testing-2.pngAnti-RUNDC1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RUNDC1 antibody (A14070). Overlay histogram showing U20S cells stained with A14070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUNDC1 Antibody (A14070, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oc90-picoband-trade-antibody-a12282-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12282-1-oc90-primary-antibodies-wb-testing-1.jpgAnti-OC90 Antibody Picoband® Western blot analysis of OC90 using anti-OC90 antibody (A12282-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OC90 antigen affinity purified polyclonal antibody (Catalog # A12282-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OC90 at approximately 52 kDa. The expected band size for OC90 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ocrl-picoband-trade-antibody-a02042-2-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02042-2-ocrl-primary-antibodies-wb-testing-1.jpgAnti-OCRL Antibody Picoband® Western blot analysis of OCRL using anti-OCRL antibody (A02042-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCRL antigen affinity purified polyclonal antibody (Catalog # A02042-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCRL at approximately 104 kDa. The expected band size for OCRL is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02042-2-ocrl-primary-antibodies-ihc-testing-2.jpgAnti-OCRL Antibody Picoband® IHC analysis of OCRL using anti-OCRL antibody (A02042-2). OCRL was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCRL Antibody (A02042-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02042-2-ocrl-primary-antibodies-ihc-testing-3.jpgAnti-OCRL Antibody Picoband® IHC analysis of OCRL using anti-OCRL antibody (A02042-2). OCRL was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCRL Antibody (A02042-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02042-2-ocrl-primary-antibodies-ihc-testing-4.jpgAnti-OCRL Antibody Picoband® IHC analysis of OCRL using anti-OCRL antibody (A02042-2). OCRL was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OCRL Antibody (A02042-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02042-2-ocrl-primary-antibodies-if-testing-5.jpgAnti-OCRL Antibody Picoband® IF analysis of OCRL using anti-OCRL antibody (A02042-2). OCRL was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OCRL Antibody (A02042-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-odf2l-picoband-trade-antibody-a16444-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16444-1-odf2l-primary-antibodies-wb-testing-1.jpgAnti-ODF2L Antibody Picoband® Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16444-1-odf2l-primary-antibodies-ihc-testing-2.jpgAnti-ODF2L Antibody Picoband® IHC analysis of ODF2L using anti-ODF2L antibody (A16444-1). ODF2L was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ODF2L Antibody (A16444-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16444-1-odf2l-primary-antibodies-fcm-testing-3.pngAnti-ODF2L Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-ODF2L antibody (A16444-1). Overlay histogram showing U937 cells stained with A16444-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ODF2L Antibody (A16444-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ogdh-picoband-trade-antibody-a05301-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05301-1-ogdh-primary-antibodies-wb-testing-1.jpgAnti-OGDH Antibody Picoband® Western blot analysis of OGDH using anti-OGDH antibody (A05301-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGDH antigen affinity purified polyclonal antibody (Catalog # A05301-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGDH at approximately 110 kDa. The expected band size for OGDH is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05301-1-ogdh-primary-antibodies-fcm-testing-2.pngAnti-OGDH Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-OGDH antibody (A05301-1). Overlay histogram showing Daudi cells stained with A05301-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGDH Antibody (A05301-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ogdhl-picoband-trade-antibody-a10713-1-boster.html2026-03-17T05:14:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10713-1-ogdhl-primary-antibodies-wb-testing-1.jpgAnti-OGDHL Antibody Picoband® Western blot analysis of OGDHL using anti-OGDHL antibody (A10713-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGDHL antigen affinity purified polyclonal antibody (Catalog # A10713-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGDHL at approximately 100 kDa. The expected band size for OGDHL is at 114 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10713-1-ogdhl-primary-antibodies-if-testing-2.jpgAnti-OGDHL Antibody Picoband® IF analysis of OGDHL using anti-OGDHL antibody (A10713-1). OGDHL was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OGDHL Antibody (A10713-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10713-1-ogdhl-primary-antibodies-fcm-testing-3.pngAnti-OGDHL Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-OGDHL antibody (A10713-1). Overlay histogram showing U937 cells stained with A10713-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGDHL Antibody (A10713-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ogfod2-picoband-trade-antibody-a16867-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16867-1-ogfod2-primary-antibodies-wb-testing-1.jpgAnti-OGFOD2 Antibody Picoband® Western blot analysis of OGFOD2 using anti-OGFOD2 antibody (A16867-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGFOD2 antigen affinity purified polyclonal antibody (Catalog # A16867-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGFOD2 at approximately 39 kDa. The expected band size for OGFOD2 is at 39,32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16867-1-ogfod2-primary-antibodies-fcm-testing-2.pngAnti-OGFOD2 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-OGFOD2 antibody (A16867-1). Overlay histogram showing Daudi cells stained with A16867-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGFOD2 Antibody (A16867-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oip5-picoband-trade-antibody-a07819-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07819-oip5-primary-antibodies-wb-testing-1.jpgAnti-OIP5 Antibody Picoband® Western blot analysis of OIP5 using anti-OIP5 antibody (A07819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIP5 antigen affinity purified polyclonal antibody (Catalog # A07819) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIP5 at approximately 22 kDa. The expected band size for OIP5 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07819-oip5-primary-antibodies-fcm-testing-2.pngAnti-OIP5 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-OIP5 antibody (A07819). Overlay histogram showing HepG2 cells stained with A07819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OIP5 Antibody (A07819, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oip5-picoband-trade-antibody-a07819-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07819-1-oip5-primary-antibodies-wb-testing-1.jpgAnti-Oip5 Antibody Picoband® Western blot analysis of Oip using anti-Oip antibody (A07819-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oip antigen affinity purified polyclonal antibody (Catalog # A07819-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oip at approximately 28 kDa. The expected band size for Oip is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-gtpbp9-ola1-picoband-trade-antibody-a07162-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07162-ola1-primary-antibodies-wb-testing-1.jpgAnti-GTPBP9/OLA1 Antibody Picoband® Western blot analysis of GTPBP9/OLA1 using anti-GTPBP9/OLA1 antibody (A07162). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTPBP9/OLA1 antigen affinity purified polyclonal antibody (Catalog # A07162) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GTPBP9/OLA1 at approximately 45 kDa. The expected band size for GTPBP9/OLA1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07162-ola1-primary-antibodies-if-testing-2.jpgAnti-GTPBP9/OLA1 Antibody Picoband® IF analysis of GTPBP9/OLA1 using anti-GTPBP9/OLA1 antibody (A07162). GTPBP9/OLA1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GTPBP9/OLA1 Antibody (A07162) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07162-ola1-primary-antibodies-fcm-testing-3.pngAnti-GTPBP9/OLA1 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-GTPBP9/OLA1 antibody (A07162). Overlay histogram showing Raji cells stained with A07162 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GTPBP9/OLA1 Antibody (A07162, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-olah-picoband-trade-antibody-a05069-3-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05069-3-olah-primary-antibodies-wb-testing-1.jpgAnti-OLAH Antibody Picoband® Western blot analysis of OLAH using anti-OLAH antibody (A05069-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLAH antigen affinity purified polyclonal antibody (Catalog # A05069-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLAH at approximately 36 kDa. The expected band size for OLAH is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05069-3-olah-primary-antibodies-if-testing-2..jpgAnti-OLAH Antibody Picoband®IF analysis of OLAH using anti-OLAH antibody (A05069-3). OLAH was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OLAH Antibody (A05069-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05069-3-olah-primary-antibodies-fcm-testing-3.pngAnti-OLAH Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-OLAH antibody (A05069-3). Overlay histogram showing HEL cells stained with A05069-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLAH Antibody (A05069-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-olfml2b-picoband-trade-antibody-a16481-2-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16481-2-olfml2b-primary-antibodies-wb-testing-1.jpgAnti-OLFML2B Antibody Picoband® Western blot analysis of OLFML2B using anti-OLFML2B antibody (A16481-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLFML2B antigen affinity purified polyclonal antibody (Catalog # A16481-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLFML2B at approximately 96 kDa. The expected band size for OLFML2B is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16481-2-olfml2b-primary-antibodies-fcm-testing-2.pngAnti-OLFML2B Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-OLFML2B antibody (A16481-2). Overlay histogram showing SiHa cells stained with A16481-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLFML2B Antibody (A16481-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-onecut1-picoband-trade-antibody-a07349-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07349-onecut1-primary-antibodies-wb-testing-1.jpgAnti-ONECUT1 Antibody Picoband® Western blot analysis of ONECUT1 using anti-ONECUT1 antibody (A07349). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT1 antigen affinity purified polyclonal antibody (Catalog # A07349) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT1 at approximately 50 kDa. The expected band size for ONECUT1 is at 51,51-55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07349-onecut1-primary-antibodies-ihc-testing-1.jpgAnti-ONECUT1 Antibody Picoband®IHC analysis of ONECUT1 using anti-ONECUT1 antibody (A07349). ONECUT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ONECUT1 Antibody (A07349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07349-onecut1-primary-antibodies-ihc-testing-2.jpgAnti-ONECUT1 Antibody Picoband®IHC analysis of ONECUT1 using anti-ONECUT1 antibody (A07349). ONECUT1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ONECUT1 Antibody (A07349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07349-onecut1-primary-antibodies-if-testing-2.jpgAnti-ONECUT1 Antibody Picoband® IF analysis of ONECUT1 and Tubulin beta using anti-ONECUT1 antibody (A07349) and anti-Tubulin beta antibody (M05613-4). ONECUT1 and Tubulin beta was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ONECUT1 Antibody (A07349) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07349-onecut1-primary-antibodies-fcm-testing-3.pngAnti-ONECUT1 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-ONECUT1 antibody (A07349). Overlay histogram showing Daudi cells stained with A07349 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ONECUT1 Antibody (A07349, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-opa1-picoband-trade-antibody-a00508-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00508-1-opa1-primary-antibodies-wb-testing-1.jpgAnti-OPA1 Antibody Picoband® Western blot analysis of OPA1 using anti-OPA1 antibody (A00508-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse eye tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (Catalog # A00508-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA1 at approximately 80-100 kDa. The expected band size for OPA1 is at 112,80-100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00508-1-opa1-primary-antibodies-if-testing-2.jpgAnti-OPA1 Antibody Picoband® IF analysis of OPA1 using anti-OPA1 antibody (A00508-1). OPA1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OPA1 Antibody (A00508-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00508-1-opa1-primary-antibodies-fcm-testing-3.pngAnti-OPA1 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-OPA1 antibody (A00508-1). Overlay histogram showing Daudi cells stained with A00508-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OPA1 Antibody (A00508-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-opa3-picoband-trade-antibody-a06345-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06345-1-opa3-primary-antibodies-wb-testing-1.jpgAnti-OPA3 Antibody Picoband® Western blot analysis of OPA3 using anti-OPA3 antibody (A06345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA3 antigen affinity purified polyclonal antibody (Catalog # A06345-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA3 at approximately 20 kDa. The expected band size for OPA3 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06345-1-opa3-primary-antibodies-fcm-testing-2.pngAnti-OPA3 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-OPA3 antibody (A06345-1). Overlay histogram showing U937 cells stained with A06345-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OPA3 Antibody (A06345-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-osbpl9-picoband-trade-antibody-a09498-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09498-1-osbpl9-primary-antibodies-wb-testing-1.jpgAnti-OSBPL9 Antibody Picoband® Western blot analysis of OSBPL9 using anti-OSBPL9 antibody (A09498-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL9 antigen affinity purified polyclonal antibody (Catalog # A09498-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL9 at approximately 95 kDa. The expected band size for OSBPL9 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09498-1-osbpl9-primary-antibodies-if-testing-2.jpgAnti-OSBPL9 Antibody Picoband® IF analysis of OSBPL9 using anti-OSBPL9 antibody (A09498-1). OSBPL9 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OSBPL9 Antibody (A09498-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09498-1-osbpl9-primary-antibodies-fcm-testing-3.pngAnti-OSBPL9 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-OSBPL9 antibody (A09498-1). Overlay histogram showing Raji cells stained with A09498-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OSBPL9 Antibody (A09498-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ostf1-picoband-trade-antibody-a05988-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05988-1-ostf1-primary-antibodies-wb-testing-1.jpgAnti-OSTF1 Antibody Picoband® Western blot analysis of OSTF1 using anti-OSTF1 antibody (A05988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSTF1 antigen affinity purified polyclonal antibody (Catalog # A05988-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSTF1 at approximately 28 kDa. The expected band size for OSTF1 is at 24,28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05988-1-ostf1-primary-antibodies-if-testing-2.jpgAnti-OSTF1 Antibody Picoband® IF analysis of OSTF1 using anti-OSTF1 antibody (A05988-1). OSTF1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OSTF1 Antibody (A05988-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05988-1-ostf1-primary-antibodies-fcm-testing-3.pngAnti-OSTF1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-OSTF1 antibody (A05988-1). Overlay histogram showing U20S cells stained with A05988-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OSTF1 Antibody (A05988-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-odf3-picoband-trade-antibody-a14065-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14065-1-odf3-primary-antibodies-wb-testing-1.jpgAnti-ODF3 Antibody Picoband® Western blot analysis of ODF3 using anti-ODF3 antibody (A14065-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF3 antigen affinity purified polyclonal antibody (Catalog # A14065-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF3 at approximately 28 kDa. The expected band size for ODF3 is at 28,30 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ssbp1-picoband-trade-antibody-a05166-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-wb-testing-1.jpgAnti-SSBP1 Antibody Picoband® Western blot analysis of SSBP1 using anti-SSBP1 ntibody (A05166-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSBP1 antigen affinity purified polyclonal antibody (Catalog # A05166-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSBP1 at approximately 16 kDa. The expected band size for SSBP1 is at 17-18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-ihc-testing-2.jpgAnti-SSBP1 Antibody Picoband® IHC analysis of SSBP1 using anti-SSBP1 antibody (A05166-1). SSBP1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSBP1 Antibody (A05166-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-ihc-testing-3.jpgAnti-SSBP1 Antibody Picoband® IHC analysis of SSBP1 using anti-SSBP1 antibody (A05166-1). SSBP1 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSBP1 Antibody (A05166-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-ihc-testing-4.jpgAnti-SSBP1 Antibody Picoband® IHC analysis of SSBP1 using anti-SSBP1 antibody (A05166-1). SSBP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSBP1 Antibody (A05166-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-ihc-testing-5.jpgAnti-SSBP1 Antibody Picoband® IHC analysis of SSBP1 using anti-SSBP1 antibody (A05166-1). SSBP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSBP1 Antibody (A05166-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-if-testing-6.jpgAnti-SSBP1 Antibody Picoband® IF analysis of SSBP1 using anti-SSBP1 antibody (A05166-1). SSBP1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SSBP1 Antibody (A05166-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-if-testing-7.jpgAnti-SSBP1 Antibody Picoband® IF analysis of SSBP1 using anti-SSBP1 antibody (A05166-1). SSBP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SSBP1 Antibody (A05166-1) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-if-testing-8.jpgAnti-SSBP1 Antibody Picoband® IF analysis of SSBP1 using anti-SSBP1 antibody (A05166-1). SSBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SSBP1 Antibody (A05166-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05166-1-ssbp1-primary-antibodies-fcm-testing-9.pngAnti-SSBP1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-SSBP1 antibody (A05166-1). Overlay histogram showing HL-60 cells stained with A05166-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSBP1 Antibody (A05166-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-trapb-ssr2-picoband-trade-antibody-a11273-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11273-1-ssr2-primary-antibodies-wb-testing-1.jpgAnti-TRAPB/SSR2 Antibody Picoband® Western blot analysis of TRAPB/SSR2 using anti-TRAPB/SSR2 antibody (A11273-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO 320 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAPB/SSR2 antigen affinity purified polyclonal antibody (Catalog # A11273-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAPB/SSR2 at approximately 22 kDa. The expected band size for TRAPB/SSR2 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11273-1-ssr2-primary-antibodies-fcm-testing-2.pngAnti-TRAPB/SSR2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-TRAPB/SSR2 antibody (A11273-1). Overlay histogram showing THP-1 cells stained with A11273-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAPB/SSR2 Antibody (A11273-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11273-1-ssr2-primary-antibodies-fcm-testing-3.pngAnti-TRAPB/SSR2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-TRAPB/SSR2 antibody (A11273-1). Overlay histogram showing U20S cells stained with A11273-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAPB/SSR2 Antibody (A11273-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-st6galnac2-picoband-trade-antibody-a10348-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10348-st6galnac2-primary-antibodies-wb-testing-1.jpgAnti-ST6GALNAC2 Antibody Picoband® Western blot analysis of ST6GALNAC2 using anti-ST6GALNAC2 antibody (A10348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST6GALNAC2 antigen affinity purified polyclonal antibody (Catalog # A10348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST6GALNAC2 at approximately 40 kDa. The expected band size for ST6GALNAC2 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-st6galnac4-picoband-trade-antibody-a11941-1-boster.html2026-03-17T05:14:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11941-1-st6galnac4-primary-antibodies-wb-testing-1.jpgAnti-ST6GALNAC4 Antibody Picoband® Western blot analysis of ST6GALNAC4 using anti-ST6GALNAC4 antibody (A11941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: rat H9C2(2-1) whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST6GALNAC4 antigen affinity purified polyclonal antibody (Catalog # A11941-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST6GALNAC4 at approximately 36 kDa. The expected band size for ST6GALNAC4 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sucla2-picoband-trade-antibody-a04807-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04807-1-sucla2-primary-antibodies-wb-testing-1.jpgAnti-SUCLA2 Antibody Picoband® Western blot analysis of SUCLA2 using anti-SUCLA2 antibody (A04807-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLA2 antigen affinity purified polyclonal antibody (Catalog # A04807-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUCLA2 at approximately 48 kDa. The expected band size for SUCLA2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04807-1-sucla2-primary-antibodies-if-testing-2.jpgAnti-SUCLA2 Antibody Picoband® IF analysis of SUCLA2 using anti-SUCLA2 antibody (A04807-1). SUCLA2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUCLA2 Antibody (A04807-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04807-1-sucla2-primary-antibodies-fcm-testing-3.pngAnti-SUCLA2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-SUCLA2 antibody (A04807-1). Overlay histogram showing THP-1 cells stained with A04807-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLA2 Antibody (A04807-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rab44-picoband-trade-antibody-a18664-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18664-rab44-primary-antibodies-wb-testing-1_1.jpgAnti-RAB44 Antibody Picoband®Western blot analysis of RAB44 using anti-RAB44 antibody (A18664). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB44 antigen affinity purified polyclonal antibody (A18664) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB44 at approximately 150 kDa. The expected band size for RAB44 is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18664-rab44-primary-antibodies-fcm-testing-2.pngAnti-RAB44 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RAB44 antibody (A18664). Overlay histogram showing HEL cells stained with A18664 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB44 Antibody (A18664, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cd48-picoband-trade-antibody-a03281-4-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03281-4-cd48-primary-antibodies-wb-testing-1.jpgAnti-Cd48 Antibody Picoband® Western blot analysis of Cd48 using anti-Cd48 antibody (A03281-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd48 antigen affinity purified polyclonal antibody (Catalog # A03281-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd48 at approximately 50 kDa. The expected band size for Cd48 is at 28,45-50 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cd11b-integrin-alpha-m-itgam-picoband-trade-antibody-a00144-2-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00144-2-itgam-primary-antibodies-wb-testing-1.jpgAnti-CD11B/Integrin Alpha M/Itgam Antibody Picoband® Western blot analysis of CD11B/Integrin Alpha M/Itgam using anti-CD11B/Integrin Alpha M/Itgam antibody (A00144-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11B/Integrin Alpha M/Itgam antigen affinity purified polyclonal antibody (Catalog # A00144-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11B/Integrin Alpha M/Itgam at approximately 170 kDa. The expected band size for CD11B/Integrin Alpha M/Itgam is at 170 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pdgfra-picoband-trade-antibody-a00366-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00366-pdgfra-primary-antibodies-wb-testing-1.jpgAnti-PDGFRA Antibody Picoband® Western blot analysis of PDGFRA using anti-PDGFRA antibody (A00366). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRA antigen affinity purified polyclonal antibody (Catalog # A00366) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFRA at approximately 180 kDa. The expected band size for PDGFRA is at 123,180 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00366-pdgfra-primary-antibodies-if-testing-2.jpgAnti-PDGFRA Antibody Picoband® IF analysis of PDGFRA using anti-PDGFRA antibody (A00366). PDGFRA was detected in an immunocytochemical section of CACO2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDGFRA Antibody (A00366) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rpl38-picoband-trade-antibody-a10156-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10156-1-rpl38-primary-antibodies-wb-testing-1.jpgAnti-RPL38 Antibody Picoband® Western blot analysis of RPL38 using anti-RPL38 antibody (A10156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL38 antigen affinity purified polyclonal antibody (Catalog # A10156-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL38 at approximately 17 kDa. The expected band size for RPL38 is at 8 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rprd1b-picoband-trade-antibody-a07903-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-wb-testing-1.jpgAnti-RPRD1B Antibody Picoband® Western blot analysis of RPRD1B using anti-RPRD1B antibody (A07903-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPRD1B antigen affinity purified polyclonal antibody (Catalog # A07903-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPRD1B at approximately 37 kDa. The expected band size for RPRD1B is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-ihc-testing-2.jpgAnti-RPRD1B Antibody Picoband® IHC analysis of RPRD1B using anti-RPRD1B antibody (A07903-1). RPRD1B was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPRD1B Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-ihc-testing-3.jpgAnti-RPRD1B Antibody Picoband® IHC analysis of RPRD1B using anti-RPRD1B antibody (A07903-1). RPRD1B was detected in a paraffin-embedded section of human B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPRD1B Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-ihc-testing-4.jpgAnti-RPRD1B Antibody Picoband® IHC analysis of RPRD1B using anti-RPRD1B antibody (A07903-1). RPRD1B was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPRD1B Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-ihc-testing-5.jpgAnti-RPRD1B Antibody Picoband® IHC analysis of RPRD1B using anti-RPRD1B antibody (A07903-1). RPRD1B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPRD1B Antibody (A07903-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-if-testing-6.jpgAnti-RPRD1B Antibody Picoband® IF analysis of RPRD1B and Tubulin beta using anti-RPRD1B antibody (A07903-1) and anti-Tubulin beta antibody (M05613-4). RPRD1B and Tubulin beta was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPRD1B Antibody (A07903-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-if-testing-7.jpgAnti-RPRD1B Antibody Picoband® IF analysis of RPRD1B using anti-RPRD1B antibody (A07903-1). RPRD1B was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RPRD1B Antibody (A07903-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07903-1-rprd1b-primary-antibodies-fcm-testing-8.pngAnti-RPRD1B Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RPRD1B antibody (A07903-1). Overlay histogram showing Hela cells stained with A07903-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPRD1B Antibody (A07903-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rps14-picoband-trade-antibody-a03696-2-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-wb-testing-1_1.jpgAnti-RPS14 Antibody Picoband® Western blot analysis of RPS14 using anti-RPS14 antibody (A03696-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 4: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS14 antigen affinity purified polyclonal antibody (Catalog # A03696-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS14 at approximately 16 kDa. The expected band size for RPS14 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-ihc-testing-2_1.jpgAnti-RPS14 Antibody Picoband® IHC analysis of RPS14 using anti-RPS14 antibody (A03696-2). RPS14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS14 Antibody (A03696-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-ihc-testing-3.jpgAnti-RPS14 Antibody Picoband® IHC analysis of RPS14 using anti-RPS14 antibody (A03696-2). RPS14 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS14 Antibody (A03696-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-ip-testing-7.jpgAnti-RPS14 Antibody Picoband® Immunoprecipitating RPS14 in Hela whole cell lysate. Western blot analysis of RPS14 using anti-RPS14 antibody (A03696-2). Lane 1: Hela whole cell lysates (30ug) Lane 2: Rabbit control IgG instead of anti-RPS14 antibody in Hela whole cell lysate. Lane 3: anti-RPS14 antibody (2μg) + Hela whole cell lysate (500μg) After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPS14 antigen affinity purified polyclonal antibody (A03696-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for RPS14 at approximately 16 kDa. The expected band size for RPS14 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-ihc-testing-4.jpgAnti-RPS14 Antibody Picoband® IHC analysis of RPS14 using anti-RPS14 antibody (A03696-2). RPS14 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS14 Antibody (A03696-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-ihc-testing-5.jpgAnti-RPS14 Antibody Picoband® IHC analysis of RPS14 using anti-RPS14 antibody (A03696-2). RPS14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS14 Antibody (A03696-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-if-testing-6.jpgAnti-RPS14 Antibody Picoband® IF analysis of RPS14 using anti-RPS14 antibody (A03696-2). RPS14 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS14 Antibody (A03696-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03696-2-rps14-primary-antibodies-fcm-testing-8.jpgAnti-RPS14 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-RPS14 antibody (A03696-2). Overlay histogram showing HepG2 cells stained with A03696-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS14 Antibody (A03696-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rreb1-picoband-trade-antibody-a03902-2-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03902-2-rreb1-primary-antibodies-wb-testing-1.jpgAnti-RREB1 Antibody Picoband® Western blot analysis of RREB1 using anti-RREB1 antibody (A03902-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RREB1 antigen affinity purified polyclonal antibody (Catalog # A03902-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RREB1 at approximately 160 kDa. The expected band size for RREB1 is at 160-181 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03902-2-rreb1-primary-antibodies-fcm-testing-2.pngAnti-RREB1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RREB1 antibody (A03902-2). Overlay histogram showing Hela cells stained with A03902-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RREB1 Antibody (A03902-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmem87a-picoband-trade-antibody-a14716-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-wb-testing-1.jpgAnti-TMEM87A Antibody Picoband® Western blot analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM87A antigen affinity purified polyclonal antibody (Catalog # A14716-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM87A at approximately 70 kDa. The expected band size for TMEM87A is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-ihc-testing-2.jpgAnti-TMEM87A Antibody Picoband® IHC analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-ihc-testing-3.jpgAnti-TMEM87A Antibody Picoband® IHC analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-ihc-testing-4.jpgAnti-TMEM87A Antibody Picoband® IHC analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-ihc-testing-5.jpgAnti-TMEM87A Antibody Picoband® IHC analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-ihc-testing-6.jpgAnti-TMEM87A Antibody Picoband® IHC analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-ihc-testing-7.jpgAnti-TMEM87A Antibody Picoband® IHC analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-ihc-testing-8.jpgAnti-TMEM87A Antibody Picoband® IHC analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-if-testing-9.jpgAnti-TMEM87A Antibody Picoband® IF analysis of TMEM87A using anti-TMEM87A antibody (A14716-1). TMEM87A was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMEM87A Antibody (A14716-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14716-1-tmem87a-primary-antibodies-fcm-testing-10.pngAnti-TMEM87A Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-TMEM87A antibody (A14716-1). Overlay histogram showing U20S cells stained with A14716-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM87A Antibody (A14716-1, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-tmem132b-picoband-trade-antibody-a16132-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16132-1-tmem132b-primary-antibodies-wb-testing-1.jpgAnti-TMEM132B Antibody Picoband® Western blot analysis of TMEM132B using anti-TMEM132B antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM132B antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM132B at approximately 95 kDa. The expected band size for TMEM132B is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16132-1-tmem132b-primary-antibodies-ihc-testing-2.jpgAnti-TMEM132B Antibody Picoband® IHC analysis of TMEM132B using anti-TMEM132B antibody (A16132-1). TMEM132B was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM132B Antibody (A16132-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16132-1-tmem132b-primary-antibodies-ihc-testing-3.jpgAnti-TMEM132B Antibody Picoband® IHC analysis of TMEM132B using anti-TMEM132B antibody (A16132-1). TMEM132B was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM132B Antibody (A16132-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16132-1-tmem132b-primary-antibodies-ihc-testing-4.jpgAnti-TMEM132B Antibody Picoband® IHC analysis of TMEM132B using anti-TMEM132B antibody (A16132-1). TMEM132B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM132B Antibody (A16132-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16132-1-tmem132b-primary-antibodies-if-testing-5.jpgAnti-TMEM132B Antibody Picoband® IF analysis of TMEM132B using anti-TMEM132B antibody (A16132-1). TMEM132B was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TMEM132B Antibody (A16132-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16132-1-tmem132b-primary-antibodies-fcm-testing-6.pngAnti-TMEM132B Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TMEM132B antibody (A16132-1). Overlay histogram showing Daudi cells stained with A16132-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM132B Antibody (A16132-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16132-1-tmem132b-primary-antibodies-fcm-testing-7.pngAnti-TMEM132B Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-TMEM132B antibody (A16132-1). Overlay histogram showing U937 cells stained with A16132-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM132B Antibody (A16132-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rufy2-picoband-trade-antibody-a13178-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13178-1-rufy2-primary-antibodies-wb-testing-1.jpgAnti-RUFY2 Antibody Picoband® Western blot analysis of RUFY2 using anti-RUFY2 antibody (A13178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY2 antigen affinity purified polyclonal antibody (Catalog # A13178-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY2 at approximately 69 kDa. The expected band size for RUFY2 is at 65-70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13178-1-rufy2-primary-antibodies-if-testing-2.jpgAnti-RUFY2 Antibody Picoband® IF analysis of RUFY2 and Tubulin beta using anti-RUFY2 antibody (A13178-1) and anti-Tubulin beta antibody (M05613-4). RUFY2 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RUFY2 Antibody (A13178-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13178-1-rufy2-primary-antibodies-fcm-testing-3.pngAnti-RUFY2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RUFY2 antibody (A13178-1). Overlay histogram showing U20S cells stained with A13178-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUFY2 Antibody (A13178-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-optc-picoband-trade-antibody-a09717-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09717-1-optc-primary-antibodies-wb-testing-1.jpgAnti-OPTC Antibody Picoband® Western blot analysis of OPTC using anti-OPTC antibody (A09717-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human ARPE-19 whole cell lysates, Lane 2: monkey RF/6A whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPTC antigen affinity purified polyclonal antibody (Catalog # A09717-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPTC at approximately 37 kDa. The expected band size for OPTC is at 37,45 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-osgin1-picoband-trade-antibody-a10009-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10009-1-osgin1-primary-antibodies-wb-testing-1.jpgAnti-OSGIN1 Antibody Picoband® Western blot analysis of OSGIN1 using anti-OSGIN1 antibody (A10009-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSGIN1 antigen affinity purified polyclonal antibody (Catalog # A10009-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSGIN1 at approximately 53 kDa. The expected band size for OSGIN1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10009-1-osgin1-primary-antibodies-ihc-testing-2.jpgAnti-OSGIN1 Antibody Picoband® IHC analysis of OSGIN1 using anti-OSGIN1 antibody (A10009-1). OSGIN1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSGIN1 Antibody (A10009-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10009-1-osgin1-primary-antibodies-ihc-testing-3.jpgAnti-OSGIN1 Antibody Picoband® IHC analysis of OSGIN1 using anti-OSGIN1 antibody (A10009-1). OSGIN1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSGIN1 Antibody (A10009-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10009-1-osgin1-primary-antibodies-ihc-testing-4.jpgAnti-OSGIN1 Antibody Picoband® IHC analysis of OSGIN1 using anti-OSGIN1 antibody (A10009-1). OSGIN1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSGIN1 Antibody (A10009-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10009-1-osgin1-primary-antibodies-ihc-testing-5.jpgAnti-OSGIN1 Antibody Picoband® IHC analysis of OSGIN1 using anti-OSGIN1 antibody (A10009-1). OSGIN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSGIN1 Antibody (A10009-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10009-1-osgin1-primary-antibodies-if-testing-6.jpgAnti-OSGIN1 Antibody Picoband® IF analysis of OSGIN1 and Tubulin beta using anti-OSGIN1 antibody (A10009-1) and anti-Tubulin beta antibody (M05613-4). OSGIN1 and Tubulin beta was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OSGIN1 Antibody (A10009-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10009-1-osgin1-primary-antibodies-if-testing-7.jpgAnti-OSGIN1 Antibody Picoband® IF analysis of OSGIN1 using anti-OSGIN1 antibody (A10009-1). OSGIN1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-OSGIN1 Antibody (A10009-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rsbn1-picoband-trade-antibody-a15336-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15336-1-rsbn1-primary-antibodies-wb-testing-1.jpgAnti-RSBN1 Antibody Picoband® Western blot analysis of RSBN1 using anti-RSBN1 antibody (A15336-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSBN1 antigen affinity purified polyclonal antibody (Catalog # A15336-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSBN1 at approximately 95 kDa. The expected band size for RSBN1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15336-1-rsbn1-primary-antibodies-ihc-testing-2.jpgAnti-RSBN1 Antibody Picoband® IHC analysis of RSBN1 using anti-RSBN1 antibody (A15336-1). RSBN1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RSBN1 Antibody (A15336-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15336-1-rsbn1-primary-antibodies-ihc-testing-3.jpgAnti-RSBN1 Antibody Picoband® IHC analysis of RSBN1 using anti-RSBN1 antibody (A15336-1). RSBN1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RSBN1 Antibody (A15336-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15336-1-rsbn1-primary-antibodies-if-testing-4.jpgAnti-RSBN1 Antibody Picoband® IF analysis of RSBN1 and Tubulin beta using anti-RSBN1 antibody (A15336-1) and anti-Tubulin beta antibody (M05613-4). RSBN1 and Tubulin beta was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RSBN1 Antibody (A15336-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15336-1-rsbn1-primary-antibodies-fcm-testing-5.pngAnti-RSBN1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-RSBN1 antibody (A15336-1). Overlay histogram showing U20S cells stained with A15336-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RSBN1 Antibody (A15336-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rsl1d1-picoband-trade-antibody-a07282-2-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07282-2-rsl1d1-primary-antibodies-wb-testing-1.jpgAnti-Rsl1d1 Antibody Picoband® Western blot analysis of Rsl1d1 using anti-Rsl1d1 antibody (A07282-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rsl1d1 antigen affinity purified polyclonal antibody (Catalog # A07282-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rsl1d1 at approximately 65 kDa. The expected band size for Rsl1d1 is at 54,55 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-oxer1-picoband-trade-antibody-a10420-1-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10420-1-oxer1-primary-antibodies-wb-testing-1.jpgAnti-OXER1 Antibody Picoband® Western blot analysis of OXER1 using anti-OXER1 antibody (A10420-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXER1 antigen affinity purified polyclonal antibody (Catalog # A10420-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXER1 at approximately 43-46 kDa. The expected band size for OXER1 is at 43-46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10420-1-oxer1-primary-antibodies-fcm-testing-2.pngAnti-OXER1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-OXER1 antibody (A10420-1). Overlay histogram showing U937 cells stained with A10420-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-OXER1 Antibody (A10420-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pacsin2-picoband-trade-antibody-a04211-2-boster.html2026-03-17T05:14:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-wb-testing-1.jpgAnti-PACSIN2 Antibody Picoband® Western blot analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat heart tissue tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN2 antigen affinity purified polyclonal antibody (Catalog # A04211-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACSIN2 at approximately 60 kDa. The expected band size for PACSIN2 is at 56,60-65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-ihc-testing-2.jpgAnti-PACSIN2 Antibody Picoband® IHC analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). PACSIN2 was detected in a paraffin-embedded section of Diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PACSIN2 Antibody (A04211-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-ihc-testing-3.jpgAnti-PACSIN2 Antibody Picoband® IHC analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). PACSIN2 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PACSIN2 Antibody (A04211-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-ihc-testing-4.jpgAnti-PACSIN2 Antibody Picoband® IHC analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). PACSIN2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PACSIN2 Antibody (A04211-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-ihc-testing-5.jpgAnti-PACSIN2 Antibody Picoband® IHC analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). PACSIN2 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PACSIN2 Antibody (A04211-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-if-testing-6.jpgAnti-PACSIN2 Antibody Picoband® IF analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). PACSIN2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PACSIN2 Antibody (A04211-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-if-testing-7.jpgAnti-PACSIN2 Antibody Picoband® IF analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). PACSIN2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PACSIN2 Antibody (A04211-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-if-testing-8.jpgAnti-PACSIN2 Antibody Picoband® IF analysis of PACSIN2 using anti-PACSIN2 antibody (A04211-2). PACSIN2 was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PACSIN2 Antibody (A04211-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04211-2-pacsin2-primary-antibodies-fcm-testing-9.pngAnti-PACSIN2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PACSIN2 antibody (A04211-2). Overlay histogram showing Hela cells stained with A04211-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PACSIN2 Antibody (A04211-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-glycodelin-paep-picoband-trade-antibody-a06051-1-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06051-1-paep-primary-antibodies-wb-testing-1.jpgAnti-Glycodelin/PAEP Antibody Picoband® Western blot analysis of Glycodelin/PAEP using anti-Glycodelin/PAEP antibody (A06051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycodelin/PAEP antigen affinity purified polyclonal antibody (Catalog # A06051-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycodelin/PAEP at approximately 24 kDa. The expected band size for Glycodelin/PAEP is at 21,24-28 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-paqr6-picoband-trade-antibody-a14355-1-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14355-1-paqr6-primary-antibodies-wb-testing-1.jpgAnti-PAQR6 Antibody Picoband® Western blot analysis of PAQR6 using anti-PAQR6 antibody (A14355-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAQR6 antigen affinity purified polyclonal antibody (Catalog # A14355-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAQR6 at approximately 38 kDa. The expected band size for PAQR6 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pate1-picoband-trade-antibody-a14959-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14959-pate1-primary-antibodies-wb-testing-1.jpgAnti-PATE1 Antibody Picoband® Western blot analysis of PATE1 using anti-PATE1 antibody (A14959). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PATE1 antigen affinity purified polyclonal antibody (Catalog # A14959) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PATE1 at approximately 14 kDa. The expected band size for PATE1 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14959-pate1-primary-antibodies-fcm-testing-2.pngAnti-PATE1 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-PATE1 antibody (A14959). Overlay histogram showing U20S cells stained with A14959 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PATE1 Antibody (A14959, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-patl2-picoband-trade-antibody-a15750-1-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15750-1-patl2-primary-antibodies-wb-testing-1.jpgAnti-PATL2 Antibody Picoband® Western blot analysis of PATL2 using anti-PATL2 antibody (A15750-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PATL2 antigen affinity purified polyclonal antibody (Catalog # A15750-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PATL2 at approximately 59 kDa. The expected band size for PATL2 is at 61,59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15750-1-patl2-primary-antibodies-if-testing-2.jpgAnti-PATL2 Antibody Picoband® IF analysis of PATL2 using anti-PATL2 antibody (A15750-1). PATL2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PATL2 Antibody (A15750-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15750-1-patl2-primary-antibodies-fcm-testing-3.pngAnti-PATL2 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PATL2 antibody (A15750-1). Overlay histogram showing HEL cells stained with A15750-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PATL2 Antibody (A15750-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-bsap-pax5-picoband-trade-antibody-a00669-2-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00669-2-pax5-primary-antibodies-wb-testing-1.jpgAnti-BSAP/PAX5 Antibody Picoband® Western blot analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BSAP/PAX5 antigen affinity purified polyclonal antibody (Catalog # A00669-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BSAP/PAX5 at approximately 45 kDa. The expected band size for BSAP/PAX5 is at 42,45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00669-2-pax5-primary-antibodies-ihc-testing-2.jpgAnti-BSAP/PAX5 Antibody Picoband® IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-2). BSAP/PAX5 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BSAP/PAX5 Antibody (A00669-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00669-2-pax5-primary-antibodies-ihc-testing-3.jpgAnti-BSAP/PAX5 Antibody Picoband® IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-2). BSAP/PAX5 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BSAP/PAX5 Antibody (A00669-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00669-2-pax5-primary-antibodies-ihc-testing-4.jpgAnti-BSAP/PAX5 Antibody Picoband® IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-2). BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BSAP/PAX5 Antibody (A00669-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00669-2-pax5-primary-antibodies-fcm-testing-5.pngAnti-BSAP/PAX5 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-BSAP/PAX5 antibody (A00669-2). Overlay histogram showing U20S cells stained with A00669-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BSAP/PAX5 Antibody (A00669-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oxct2-picoband-trade-antibody-a15113-1-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15113-1-oxct2-primary-antibodies-wb-testing-1.jpgAnti-OXCT2 Antibody Picoband® Western blot analysis of OXCT2 using anti-OXCT2 antibody (A15113-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXCT2 antigen affinity purified polyclonal antibody (Catalog # A15113-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXCT2 at approximately 56 kDa. The expected band size for OXCT2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15113-1-oxct2-primary-antibodies-ihc-testing-2.jpgAnti-OXCT2 Antibody Picoband® IHC analysis of OXCT2 using anti-OXCT2 antibody (A15113-1). OXCT2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OXCT2 Antibody (A15113-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15113-1-oxct2-primary-antibodies-if-testing-3.jpgAnti-OXCT2 Antibody Picoband® IF analysis of OXCT2 using anti-OXCT2 antibody (A15113-1). OXCT2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OXCT2 Antibody (A15113-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15113-1-oxct2-primary-antibodies-fcm-testing-4.pngAnti-OXCT2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-OXCT2 antibody (A15113-1). Overlay histogram showing Hela cells stained with A15113-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXCT2 Antibody (A15113-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oxsm-picoband-trade-antibody-a12866-1-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12866-1-oxsm-primary-antibodies-wb-testing-1.jpgAnti-OXSM Antibody Picoband® Western blot analysis of OXSM using anti-OXSM antibody (A12866-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSM antigen affinity purified polyclonal antibody (Catalog # A12866-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXSM at approximately 46 kDa. The expected band size for OXSM is at 46,49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12866-1-oxsm-primary-antibodies-ihc-testing-2.jpgAnti-OXSM Antibody Picoband® IHC analysis of OXSM using anti-OXSM antibody (A12866-1). OXSM was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OXSM Antibody (A12866-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12866-1-oxsm-primary-antibodies-ihc-testing-3.jpgAnti-OXSM Antibody Picoband® IHC analysis of OXSM using anti-OXSM antibody (A12866-1). OXSM was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OXSM Antibody (A12866-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12866-1-oxsm-primary-antibodies-ihc-testing-4.jpgAnti-OXSM Antibody Picoband® IHC analysis of OXSM using anti-OXSM antibody (A12866-1). OXSM was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OXSM Antibody (A12866-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12866-1-oxsm-primary-antibodies-ihc-testing-5.jpgAnti-OXSM Antibody Picoband® IHC analysis of OXSM using anti-OXSM antibody (A12866-1). OXSM was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OXSM Antibody (A12866-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12866-1-oxsm-primary-antibodies-if-testing-6.jpgAnti-OXSM Antibody Picoband® IF analysis of OXSM using anti-OXSM antibody (A12866-1). OXSM was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OXSM Antibody (A12866-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12866-1-oxsm-primary-antibodies-fcm-testing-7.pngAnti-OXSM Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-OXSM antibody (A12866-1). Overlay histogram showing MCF-7 cells stained with A12866-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXSM Antibody (A12866-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pitpnb-picoband-trade-antibody-a11315-2-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-wb-testing-1.jpgAnti-PITPNB Antibody Picoband® Western blot analysis of PITPNB using anti-PITPNB antibody (A11315-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tisue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITPNB antigen affinity purified polyclonal antibody (Catalog # A11315-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITPNB at approximately 35 kDa. The expected band size for PITPNB is at 32,35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-ihc-testing-2.jpgAnti-PITPNB Antibody Picoband® IHC analysis of PITPNB using anti-PITPNB antibody (A11315-2). PITPNB was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITPNB Antibody (A11315-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-ihc-testing-3.jpgAnti-PITPNB Antibody Picoband® IHC analysis of PITPNB using anti-PITPNB antibody (A11315-2). PITPNB was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITPNB Antibody (A11315-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-ihc-testing-4.jpgAnti-PITPNB Antibody Picoband® IHC analysis of PITPNB using anti-PITPNB antibody (A11315-2). PITPNB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITPNB Antibody (A11315-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-ihc-testing-5.jpgAnti-PITPNB Antibody Picoband® IHC analysis of PITPNB using anti-PITPNB antibody (A11315-2). PITPNB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PITPNB Antibody (A11315-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-if-testing-6.jpgAnti-PITPNB Antibody Picoband® IF analysis of PITPNB using anti-PITPNB antibody (A11315-2). PITPNB was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PITPNB Antibody (A11315-2) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-fcm-testing-7.pngAnti-PITPNB Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PITPNB antibody (A11315-2). Overlay histogram showing Hela cells stained with A11315-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITPNB Antibody (A11315-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11315-2-pitpnb-primary-antibodies-fcm-testing-8.pngAnti-PITPNB Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PITPNB antibody (A11315-2). Overlay histogram showing Hela cells stained with A11315-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITPNB Antibody (A11315-2, 1 μg/1x106 cells) for 30 min at 20°C. PE conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pln-picoband-trade-antibody-a01395-2-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01395-2-pln-primary-antibodies-wb-testing-1.jpgAnti-PLN Antibody Picoband® Western blot analysis of PLN using anti-PLN antibody (A01395-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLN antigen affinity purified polyclonal antibody (Catalog # A01395-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLN at approximately 10,22 kDa. The expected band size for PLN is at 6 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tmem30b-picoband-trade-antibody-a13764-3-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13764-3-tmem30b-primary-antibodies-wb-testing-1.jpgAnti-TMEM30B Antibody Picoband® Western blot analysis of TMEM30B using anti-TMEM30B antibody (A13764-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM30B antigen affinity purified polyclonal antibody (Catalog # A13764-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM30B at approximately 39 kDa. The expected band size for TMEM30B is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-tsen54-picoband-trade-antibody-a07022-2-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07022-2-tsen54-primary-antibodies-wb-testing-1.jpgAnti-TSEN54 Antibody Picoband® Western blot analysis of TSEN54 using anti-TSEN54 antibody (A07022-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSEN54 antigen affinity purified polyclonal antibody (Catalog # A07022-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSEN54 at approximately 59 kDa. The expected band size for TSEN54 is at 59,20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07022-2-tsen54-primary-antibodies-ihc-testing-2.jpgAnti-TSEN54 Antibody Picoband® IHC analysis of TSEN54 using anti-TSEN54 antibody (A07022-2). TSEN54 was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSEN54 Antibody (A07022-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07022-2-tsen54-primary-antibodies-if-testing-3.jpgAnti-TSEN54 Antibody Picoband® IF analysis of TSEN54 and Tubulin beta using anti-TSEN54 antibody (A07022-2) and anti-Tubulin beta antibody (M05613-4). TSEN54 and Tubulin beta was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSEN54 Antibody (A07022-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07022-2-tsen54-primary-antibodies-fcm-testing-4.pngAnti-TSEN54 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-TSEN54 antibody (A07022-2). Overlay histogram showing Hela cells stained with A07022-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSEN54 Antibody (A07022-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pgam1-picoband-trade-antibody-a04470-2-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04470-2-pgam1-primary-antibodies-wb-testing-1_1.jpgAnti-PGAM1 Antibody Picoband®Western blot analysis of PGAM1 using anti-PGAM1 antibody (A04470-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGAM1 antigen affinity purified polyclonal antibody (Catalog # A04470-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGAM1 at approximately 29 kDa. The expected band size for PGAM1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04470-2-pgam1-primary-antibodies-wb-testing-2.jpgAnti-PGAM1 Antibody Picoband®Western blot analysis of PGAM1 using anti-PGAM1 antibody (A04470-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGAM1 antigen affinity purified polyclonal antibody (Catalog # A04470-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody (Catalog # BA1150) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGAM1 at approximately 29 kDa. The expected band size for PGAM1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04470-2-pgam1-primary-antibodies-ihc-testing-1.jpgAnti-PGAM1 Antibody Picoband®IHC analysis of PGAM1 using anti-PGAM1 antibody (A04470-2). PGAM1 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGAM1 Antibody (A04470-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04470-2-pgam1-primary-antibodies-ihc-testing-2.jpgAnti-PGAM1 Antibody Picoband®IHC analysis of PGAM1 using anti-PGAM1 antibody (A04470-2). PGAM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGAM1 Antibody (A04470-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04470-2-pgam1-primary-antibodies-if-testing-1.jpgAnti-PGAM1 Antibody Picoband®IF analysis of PGAM1 using anti-PGAM1 antibody (A04470-2). PGAM1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PGAM1 Antibody (A04470-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04470-2-pgam1-primary-antibodies-fcm-testing-1.jpgAnti-PGAM1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-PGAM1 antibody (A04470-2). Overlay histogram showing 293T cells stained with A04470-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGAM1 Antibody (A04470-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pfn3-picoband-trade-antibody-a13729-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13729-pfn3-primary-antibodies-wb-testing-1.jpgAnti-PFN3 Antibody Picoband® Western blot analysis of PFN3 using anti-PFN3 antibody (A13729). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFN3 antigen affinity purified polyclonal antibody (Catalog # A13729) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFN3 at approximately 15 kDa. The expected band size for PFN3 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13729-pfn3-primary-antibodies-if-testing-2.jpgAnti-PFN3 Antibody Picoband® IF analysis of PFN3 using anti-PFN3 antibody (A13729). PFN3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PFN3 Antibody (A13729) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13729-pfn3-primary-antibodies-fcm-testing-3.pngAnti-PFN3 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PFN3 antibody (A13729). Overlay histogram showing HEL cells stained with A13729 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFN3 Antibody (A13729, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-rtl3-picoband-trade-antibody-a31819-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31819-rtl3-primary-antibodies-wb-testing-1.jpgAnti-RTL3 Antibody Picoband® Western blot analysis of RTL3 using anti-RTL3 antibody (A31819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL3 antigen affinity purified polyclonal antibody (Catalog # A31819) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTL3 at approximately 50 kDa. The expected band size for RTL3 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31819-rtl3-primary-antibodies-fcm-testing-2.pngAnti-RTL3 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-RTL3 antibody (A31819). Overlay histogram showing THP-1 cells stained with A31819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTL3 Antibody (A31819, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-osbpl8-picoband-trade-antibody-a08408-2-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08408-2-osbpl8-primary-antibodies-wb-testing-1.jpgAnti-OSBPL8 Antibody Picoband® Western blot analysis of OSBPL8 using anti-OSBPL8 antibody (A08408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL8 antigen affinity purified polyclonal antibody (Catalog # A08408-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL8 at approximately 120 kDa. The expected band size for OSBPL8 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08408-2-osbpl8-primary-antibodies-if-testing-2.jpgAnti-OSBPL8 Antibody Picoband® IF analysis of OSBPL8 using anti-OSBPL8 antibody (A08408-2). OSBPL8 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OSBPL8 Antibody (A08408-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08408-2-osbpl8-primary-antibodies-fcm-testing-3.pngAnti-OSBPL8 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-OSBPL8 antibody (A08408-2). Overlay histogram showing HepG2 cells stained with A08408-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OSBPL8 Antibody (A08408-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oit3-picoband-trade-antibody-a12768-1-boster.html2026-03-17T05:14:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12768-1-oit3-primary-antibodies-wb-testing-1.jpgAnti-OIT3 Antibody Picoband® Western blot analysis of OIT3 using anti-OIT3 antibody (A12768-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human hepatocellular carcinoma tumor tissue(HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue(HCCP) lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OIT3 antigen affinity purified polyclonal antibody (Catalog # A12768-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OIT3 at approximately 51 kDa. The expected band size for OIT3 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12768-1-oit3-primary-antibodies-fcm-testing-2.pngAnti-OIT3 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-OIT3 antibody (A12768-1). Overlay histogram showing CACO-2 cells stained with A12768-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OIT3 Antibody (A12768-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oplah-picoband-trade-antibody-a11695-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11695-1-oplah-primary-antibodies-wb-testing-1.jpgAnti-OPLAH Antibody Picoband® Western blot analysis of OPLAH using anti-OPLAH antibody (A11695-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPLAH antigen affinity purified polyclonal antibody (Catalog # A11695-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPLAH at approximately 140 kDa. The expected band size for OPLAH is at 135-140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11695-1-oplah-primary-antibodies-if-testing-2.jpgAnti-OPLAH Antibody Picoband® IF analysis of OPLAH using anti-OPLAH antibody (A11695-1). OPLAH was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OPLAH Antibody (A11695-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11695-1-oplah-primary-antibodies-fcm-testing-3.pngAnti-OPLAH Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-OPLAH antibody (A11695-1). Overlay histogram showing HEL cells stained with A11695-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OPLAH Antibody (A11695-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-smcr8-picoband-trade-antibody-a14158-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14158-1-smcr8-primary-antibodies-wb-testing-1.jpgAnti-SMCR8 Antibody Picoband® Western blot analysis of SMCR8 using anti-SMCR8 antibody (A14158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMCR8 antigen affinity purified polyclonal antibody (Catalog # A14158-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMCR8 at approximately 140 kDa. The expected band size for SMCR8 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14158-1-smcr8-primary-antibodies-fcm-testing-2.pngAnti-SMCR8 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SMCR8 antibody (A14158-1). Overlay histogram showing HEL cells stained with A14158-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMCR8 Antibody (A14158-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-c19orf61-smg9-picoband-trade-antibody-a10233-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10233-1-smg9-primary-antibodies-wb-testing-1.jpgAnti-C19orf61/SMG9 Antibody Picoband® Western blot analysis of C19orf61/SMG9 using anti-C19orf61/SMG9 antibody (A10233-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf61/SMG9 antigen affinity purified polyclonal antibody (Catalog # A10233-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf61/SMG9 at approximately 58 kDa. The expected band size for C19orf61/SMG9 is at 57-65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10233-1-smg9-primary-antibodies-if-testing-2.jpgAnti-C19orf61/SMG9 Antibody Picoband® IF analysis of C19orf61/SMG9 using anti-C19orf61/SMG9 antibody (A10233-1). C19orf61/SMG9 was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C19orf61/SMG9 Antibody (A10233-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10233-1-smg9-primary-antibodies-fcm-testing-3.pngAnti-C19orf61/SMG9 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-C19orf61/SMG9 antibody (A10233-1). Overlay histogram showing HepG2 cells stained with A10233-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf61/SMG9 Antibody (A10233-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-sulf2-picoband-trade-antibody-a03903-2-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03903-2-sulf2-primary-antibodies-wb-testing-1.jpgAnti-SULF2 Antibody Picoband® Western blot analysis of SULF2 using anti-SULF2 antibody (A03903-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULF2 antigen affinity purified polyclonal antibody (Catalog # A03903-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULF2 at approximately 135 kDa. The expected band size for SULF2 is at 100,130-135 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-eif4enif1-picoband-trade-antibody-a05520-2-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05520-2-eif4enif1-primary-antibodies-wb-testing-1.jpgAnti-EIF4ENIF1 Antibody Picoband® Western blot analysis of EIF4ENIF1 using anti-EIF4ENIF1 antibody (A05520-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse brain tissue lysates. Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4ENIF1 antigen affinity purified polyclonal antibody (Catalog # A05520-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4ENIF1 at approximately 140 kDa. The expected band size for EIF4ENIF1 is at 108,140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05520-2-eif4enif1-primary-antibodies-if-testing-1.jpgAnti-EIF4ENIF1 Antibody Picoband®IF analysis of EIF4ENIF1 using anti-EIF4ENIF1 antibody (A05520-2). EIF4ENIF1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EIF4ENIF1 Antibody (A05520-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05520-2-eif4enif1-primary-antibodies-fcm-testing-2.pngAnti-EIF4ENIF1 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-EIF4ENIF1 antibody (A05520-2). Overlay histogram showing HL-60 cells stained with A05520-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4ENIF1 Antibody (A05520-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pccb-picoband-trade-antibody-a04326-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04326-1-pccb-primary-antibodies-wb-testing-1.jpgAnti-PCCB Antibody Picoband® Western blot analysis of PCCB using anti-PCCB antibody (A04326-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCCB antigen affinity purified polyclonal antibody (Catalog # A04326-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCCB at approximately 55 kDa. The expected band size for PCCB is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04326-1-pccb-primary-antibodies-ihc-testing-2.jpgAnti-PCCB Antibody Picoband® IHC analysis of PCCB using anti-PCCB antibody (A04326-1). PCCB was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCCB Antibody (A04326-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04326-1-pccb-primary-antibodies-ihc-testing-3.jpgAnti-PCCB Antibody Picoband® IHC analysis of PCCB using anti-PCCB antibody (A04326-1). PCCB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCCB Antibody (A04326-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04326-1-pccb-primary-antibodies-if-testing-1.jpgAnti-PCCB Antibody Picoband®IF analysis of PCCB using anti-PCCB antibody (A04326-1). PCCB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PCCB Antibody (A04326-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04326-1-pccb-primary-antibodies-fcm-testing-4.pngAnti-PCCB Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PCCB antibody (A04326-1). Overlay histogram showing HepG2 cells stained with A04326-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCCB Antibody (A04326-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pdxdc1-picoband-trade-antibody-a11375-2-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11375-2-pdxdc1-primary-antibodies-wb-testing-1_1.jpgAnti-PDXDC1 Antibody Picoband®Western blot analysis of PDXDC1 using anti-PDXDC1 antibody (A11375-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat restis tissue lysates, Lane 5: mouse restis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXDC1 antigen affinity purified polyclonal antibody (A11375-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDXDC1 at approximately 87 kDa. The expected band size for PDXDC1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11375-2-pdxdc1-primary-antibodies-ihc-testing-1.jpgAnti-PDXDC1 Antibody Picoband®IHC analysis of PDXDC1 using anti-PDXDC1 antibody (A11375-2). PDXDC1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDXDC1 Antibody (A11375-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11375-2-pdxdc1-primary-antibodies-ihc-testing-2_1.jpgAnti-PDXDC1 Antibody Picoband®IHC analysis of PDXDC1 using anti-PDXDC1 antibody (A11375-2). PDXDC1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDXDC1 Antibody (A11375-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11375-2-pdxdc1-primary-antibodies-if-testing-1.jpgAnti-PDXDC1 Antibody Picoband®IF analysis of PDXDC1 using anti-PDXDC1 antibody (A11375-2) and anti-Alpha Tubulin antibody (M03989-3). PDXDC1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDXDC1 Antibody (A11375-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1127) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11375-2-pdxdc1-primary-antibodies-ip-testing-1.jpgAnti-PDXDC1 Antibody Picoband®Immunoprecipitating (IP) PDXDC1 in Hela whole cell lysate. Western blot analysis of PDXDC1 using anti-PDXDC1 antibody (A11375-2); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PDXDC1 antibody in Hela whole cell lysate; Lane 3: anti-PDXDC1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PDXDC1 antigen affinity purified polyclonal antibody (A11375-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PDXDC1 at approximately 87 kDa. The expected band size for PDXDC1 is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pik3r6-picoband-trade-antibody-a14338-1boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14338-1-pik3r6-primary-antibodies-wb-testing-1.jpgAnti-PIK3R6 Antibody Picoband® Western blot analysis of PIK3R6 using anti-PIK3R6 antibody (A14338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R6 antigen affinity purified polyclonal antibody (Catalog # A14338-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R6 at approximately 84 kDa. The expected band size for PIK3R6 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14338-1-pik3r6-primary-antibodies-fcm-testing-2.pngAnti-PIK3R6 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PIK3R6 antibody (A14338-1). Overlay histogram showing HEL cells stained with A14338-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIK3R6 Antibody (A14338-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-plcd3-picoband-trade-antibody-a06765boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06765-plcd3-primary-antibodies-wb-testing-1.jpgAnti-PLCD3 Antibody Picoband® Western blot analysis of PLCD3 using anti-PLCD3 antibody (A06765). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCD3 antigen affinity purified polyclonal antibody (Catalog # A06765) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCD3 at approximately 94 kDa. The expected band size for PLCD3 is at 110,85-90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06765-plcd3-primary-antibodies-if-testing-2.jpgAnti-PLCD3 Antibody Picoband® IF analysis of PLCD3 using anti-PLCD3 antibody (A06765). PLCD3 was detected in an immunocytochemical section of CACO2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLCD3 Antibody (A06765) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06765-plcd3-primary-antibodies-fcm-testing-3.pngAnti-PLCD3 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-PLCD3 antibody (A06765). Overlay histogram showing HL-60 cells stained with A06765 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLCD3 Antibody (A06765, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-svopl-picoband-trade-antibody-a16758-1boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16758-1-svopl-primary-antibodies-wb-testing-1.jpgAnti-SVOPL Antibody Picoband® Western blot analysis of SVOPL using anti-SVOPL antibody (A16758-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SVOPL antigen affinity purified polyclonal antibody (Catalog # A16758-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SVOPL at approximately 37 kDa. The expected band size for SVOPL is at 54,37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16758-1-svopl-primary-antibodies-fcm-testing-2.pngAnti-SVOPL Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SVOPL antibody (A16758-1). Overlay histogram showing HEL cells stained with A16758-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SVOPL Antibody (A16758-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pgp-picoband-trade-antibody-a00932-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-wb-testing-1.jpgAnti-PGP Antibody Picoband® Western blot analysis of PGP using anti-PGP antibody (A00932-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP antigen affinity purified polyclonal antibody (Catalog # A00932-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGP at approximately 36 kDa. The expected band size for PGP is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-12951_2025_3525_fig7_html.pngAnti-PGP Antibody Picoband®Effects of ZT/β-HgS on BBB permeability. (A) Effect of ZT/β-HgS on HBMECs intercellular connexins (ZO-1, occludin, and VE-cadherin) at 48 h. (B) Western blots did not show that the expressions of connexins in HBMECs were affected by ZT/β-HgS. (C) The expressions of connexins in brain were quantitatively analyzed by confocal laser scanning microscopy. (D) Western blots and quantitative analysis found that ZT/β-HgS had no effect on the expressions of connexins in mouse brain. (E) Physical disruption of the BBB was characterized by Evans blue (EB) penetration assay. (F) Effects of ZT/β-HgS on transporters in HBMECs were evaluated by immunocytochemistry ( n = 3). (G - H) The expression of transporters in cells and brain were evaluated by Western blot ( n = 3). (I - J) Effects of ZT/β-HgS on transporters in microvessels of BBB in mice ( n = 6). Double-labeled immunostaining of caveolin, clathrin, dynamin and P-gp in mouse brain frozen sections. Target proteins were labeled with DyLight 594(Red). Nuclei were stained with DAPI (blue signal), and blood vessels were labeled with fluorescein isothiocyanate lectin (green signal). Data were presented as means ± SEM. Experiment performed at least three times. One-way ANOVA was used to analyze the differences between groups. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to Con Full size image Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03525-5'>40524151</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-ihc-testing-2.jpgAnti-PGP Antibody Picoband® IHC analysis of PGP using anti-PGP antibody (A00932-1). PGP was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGP Antibody (A00932-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-ihc-testing-3.jpgAnti-PGP Antibody Picoband® IHC analysis of PGP using anti-PGP antibody (A00932-1). PGP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGP Antibody (A00932-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-ihc-testing-4.jpgAnti-PGP Antibody Picoband® IHC analysis of PGP using anti-PGP antibody (A00932-1). PGP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGP Antibody (A00932-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-ihc-testing-5.jpgAnti-PGP Antibody Picoband® IHC analysis of PGP using anti-PGP antibody (A00932-1). PGP was detected in a paraffin-embedded section of human sophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGP Antibody (A00932-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-if-testing-6.jpgAnti-PGP Antibody Picoband® IF analysis of PGP using anti-PGP antibody (A00932-1). PGP was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PGP Antibody (A00932-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-fcm-testing-7.pngAnti-PGP Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-PGP antibody (A00932-1). Overlay histogram showing PC-3 cells stained with A00932-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGP Antibody (A00932-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-if-testing-8.jpgAnti-PGP Antibody Picoband® IF analysis of PGP using anti-PGP antibody (A00932-1). PGP was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PGP Antibody (A00932-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00932-1-pgp-primary-antibodies-if-testing-9.jpgAnti-PGP Antibody Picoband® IF analysis of PGP using anti-PGP antibody (A00932-1). PGP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PGP Antibody (A00932-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rab39a-b-picoband-trade-antibody-a11551-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11551-1-rab39ab-primary-antibodies-wb-testing-1.jpgAnti-RAB39A/B Antibody Picoband® Western blot analysis of RAB39A/B using anti-RAB39A/B antibody (A11551-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB39A/B antigen affinity purified polyclonal antibody (Catalog # A11551-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB39A/B at approximately 20-24 kDa. The expected band size for RAB39A/B is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11551-1-rab39ab-primary-antibodies-if-testing-2.jpgAnti-RAB39A/B Antibody Picoband® IF analysis of RAB39A/B using anti-RAB39A/B antibody (A11551-1). RAB39A/B was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB39A/B Antibody (A11551-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11551-1-rab39ab-primary-antibodies-fcm-testing-3.pngAnti-RAB39A/B Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-RAB39A/B antibody (A11551-1). Overlay histogram showing 293T cells stained with A11551-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB39A/B Antibody (A11551-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-rab43-picoband-trade-antibody-a09315-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09315-1-rab43-primary-antibodies-wb-testing-1.jpgAnti-RAB43 Antibody Picoband® Western blot analysis of RAB43 using anti-RAB43 antibody (A09315-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB43 antigen affinity purified polyclonal antibody (Catalog # A09315-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB43 at approximately 23 kDa. The expected band size for RAB43 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09315-1-rab43-primary-antibodies-if-testing-2.jpgAnti-RAB43 Antibody Picoband® IF analysis of RAB43 using anti-RAB43 antibody (A09315-1). RAB43 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB43 Antibody (A09315-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09315-1-rab43-primary-antibodies-fcm-testing-3.pngAnti-RAB43 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RAB43 antibody (A09315-1). Overlay histogram showing HL-60 cells stained with A09315-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB43 Antibody (A09315-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-caspr2-cntnap2-picoband-trade-antibody-a02819-2-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02819-2-cntnap2-primary-antibodies-wb-testing-1.jpgAnti-Caspr2/CNTNAP2 Antibody Picoband® Western blot analysis of Caspr2/CNTNAP2 using anti-Caspr2/CNTNAP2 antibody (A02819-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspr2/CNTNAP2 antigen affinity purified polyclonal antibody (Catalog # A02819-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspr2/CNTNAP2 at approximately 170 kDa. The expected band size for Caspr2/CNTNAP2 is at 170 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-rbp2-picoband-trade-antibody-a06064-1-boster.html2026-03-17T05:14:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06064-1-rbp2-primary-antibodies-wb-testing-1.jpgAnti-RBP2 Antibody Picoband® Western blot analysis of RBP2 using anti-RBP2 antibody (A06064-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat small intestines tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestines tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP2 antigen affinity purified polyclonal antibody (Catalog # A06064-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBP2 at approximately 16 kDa. The expected band size for RBP2 is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-recoverin-rcvrn-picoband-trade-antibody-a06882-2-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06882-2-rcvrn-primary-antibodies-wb-testing-1.jpgAnti-Recoverin/RCVRN Antibody Picoband® Western blot analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (A06882-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human ARPE-19 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Recoverin/RCVRN antigen affinity purified polyclonal antibody (Catalog # A06882-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Recoverin/RCVRN at approximately 26kDa. The expected band size for Recoverin/RCVRN is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06882-2-rcvrn-primary-antibodies-ihc-testing-2.jpgAnti-Recoverin/RCVRN Antibody Picoband® IHC analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (A06882-2). Recoverin/RCVRN was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Recoverin/RCVRN Antibody (A06882-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06882-2-rcvrn-primary-antibodies-ihc-testing-3.jpgAnti-Recoverin/RCVRN Antibody Picoband® IHC analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (A06882-2). Recoverin/RCVRN was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Recoverin/RCVRN Antibody (A06882-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06882-2-rcvrn-primary-antibodies-ihc-testing-4.jpgAnti-Recoverin/RCVRN Antibody Picoband® IHC analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (A06882-2). Recoverin/RCVRN was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Recoverin/RCVRN Antibody (A06882-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06882-2-rcvrn-primary-antibodies-ihc-testing-5.jpgAnti-Recoverin/RCVRN Antibody Picoband® IHC analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (A06882-2). Recoverin/RCVRN was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Recoverin/RCVRN Antibody (A06882-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06882-2-rcvrn-primary-antibodies-ihc-testing-6.jpgAnti-Recoverin/RCVRN Antibody Picoband® IHC analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (A06882-2). Recoverin/RCVRN was detected in a paraffin-embedded section of human uterus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Recoverin/RCVRN Antibody (A06882-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-rbm15-picoband-trade-antibody-a04395-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-wb-testing-1_1.jpgAnti-RBM15 Antibody Picoband®Western blot analysis of RBM15 using anti-RBM15 antibody (A04395). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM15 antigen affinity purified polyclonal antibody (A04395) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM15 at approximately 107 kDa. The expected band size for RBM15 is at 107 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-ihc-testing-1.jpgAnti-RBM15 Antibody Picoband®IHC analysis of RBM15 using anti-RBM15 antibody (A04395). RBM15 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15 Antibody (A04395) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-ihc-testing-2_1.jpgAnti-RBM15 Antibody Picoband®IHC analysis of RBM15 using anti-RBM15 antibody (A04395). RBM15 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15 Antibody (A04395) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-ihc-testing-3_1.jpgAnti-RBM15 Antibody Picoband®IHC analysis of RBM15 using anti-RBM15 antibody (A04395). RBM15 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15 Antibody (A04395) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-ihc-testing-4_1.jpgAnti-RBM15 Antibody Picoband®IHC analysis of RBM15 using anti-RBM15 antibody (A04395). RBM15 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM15 Antibody (A04395) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-if-testing-1.jpgAnti-RBM15 Antibody Picoband®IF analysis of RBM15 using anti-RBM15 antibody (A04395) and anti-Tubulin Alpha antibody (M03989-3). RBM15 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBM15 Antibody (A04395) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-if-testing-2.jpgAnti-RBM15 Antibody Picoband®IF analysis of RBM15 using anti-RBM15 antibody (A04395). RBM15 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBM15 Antibody (A04395) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04395-rbm15-primary-antibodies-fcm-testing-1.jpgAnti-RBM15 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-RBM15 antibody (A04395). Overlay histogram showing 293T cells stained with A04395 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15 Antibody (A04395, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-renin-ren-picoband-trade-antibody-a03689-3-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03689-3-ren-primary-antibodies-wb-testing-1.jpgAnti-Renin/REN Antibody Picoband® Western blot analysis of Renin/REN using anti-Renin/REN antibody (A03689-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HUH-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Renin/REN antigen affinity purified polyclonal antibody (Catalog # A03689-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Renin/REN at approximately 55 kDa. The expected band size for Renin/REN is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03689-3-ren-primary-antibodies-ihc-testing-2.jpgAnti-Renin/REN Antibody Picoband® IHC analysis of Renin/REN using anti-Renin/REN antibody (A03689-3). Renin/REN was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Renin/REN Antibody (A03689-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03689-3-ren-primary-antibodies-ihc-testing-3.jpgAnti-Renin/REN Antibody Picoband® IHC analysis of Renin/REN using anti-Renin/REN antibody (A03689-3). Renin/REN was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Renin/REN Antibody (A03689-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03689-3-ren-primary-antibodies-ihc-testing-4.jpgAnti-Renin/REN Antibody Picoband® IHC analysis of Renin/REN using anti-Renin/REN antibody (A03689-3). Renin/REN was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Renin/REN Antibody (A03689-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-riok2-picoband-trade-antibody-a09743-1-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09743-1-riok2-primary-antibodies-wb-testing-1.jpgAnti-RIOK2 Antibody Picoband® Western blot analysis of RIOK2 using anti-RIOK2 antibody (A09743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK2 antigen affinity purified polyclonal antibody (Catalog # A09743-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIOK2 at approximately 63 kDa. The expected band size for RIOK2 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09743-1-riok2-primary-antibodies-fcm-testing-2.pngAnti-RIOK2 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RIOK2 antibody (A09743-1). Overlay histogram showing HL-60 cells stained with A09743-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIOK2 Antibody (A09743-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-rps13-picoband-trade-antibody-a06221-3-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06221-3-rps13-primary-antibodies-wb-testing-1.jpgAnti-RPS13 Antibody Picoband® Western blot analysis of RPS13 using anti-RPS13 antibody (A06221-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS13 antigen affinity purified polyclonal antibody (Catalog # A06221-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS13 at approximately 17 kDa. The expected band size for RPS13 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06221-3-rps13-primary-antibodies-ihc-testing-2.jpgAnti-RPS13 Antibody Picoband® IHC analysis of RPS13 using anti-RPS13 antibody (A06221-3). RPS13 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS13 Antibody (A06221-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06221-3-rps13-primary-antibodies-ihc-testing-3.jpgAnti-RPS13 Antibody Picoband® IHC analysis of RPS13 using anti-RPS13 antibody (A06221-3). RPS13 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS13 Antibody (A06221-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06221-3-rps13-primary-antibodies-ihc-testing-4.jpgAnti-RPS13 Antibody Picoband® IHC analysis of RPS13 using anti-RPS13 antibody (A06221-3). RPS13 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPS13 Antibody (A06221-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06221-3-rps13-primary-antibodies-fcm-testing-5.pngAnti-RPS13 Antibody Picoband® Flow Cytometry analysis of HL-60 cells using anti-RPS13 antibody (A06221-3). Overlay histogram showing HL-60 cells stained with A06221-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS13 Antibody (A06221-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-rras-picoband-trade-antibody-a04310-1-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04310-1-rras-primary-antibodies-wb-testing-1.jpgAnti-RRAS Antibody Picoband® Western blot analysis of RRAS using anti-RRAS antibody (A04310-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRAS antigen affinity purified polyclonal antibody (Catalog # A04310-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRAS at approximately 23 kDa. The expected band size for RRAS is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04310-1-rras-primary-antibodies-fcm-testing-2.pngAnti-RRAS Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-RRAS antibody (A04310-1). Overlay histogram showing HEL cells stained with A04310-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRAS Antibody (A04310-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pan2-picoband-trade-antibody-a03834-1-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03834-1-pan2-primary-antibodies-wb-testing-1.jpgAnti-PAN2 Antibody Picoband® Western blot analysis of PAN2 using anti-PAN2 antibody (A03834-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hel whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAN2 antigen affinity purified polyclonal antibody (Catalog # A03834-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAN2 at approximately 135 kDa. The expected band size for PAN2 is at 135 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03834-1-pan2-primary-antibodies-fcm-testing-2.pngAnti-PAN2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-PAN2 antibody (A03834-1). Overlay histogram showing PC-3 cells stained with A03834-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAN2 Antibody (A03834-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-asc-tms1-pycard-picoband-trade-antibody-a00362-5-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-5-pycard-primary-antibodies-wb-testing-1.jpgAnti-ASC/TMS1/Pycard Antibody Picoband® Western blot analysis of ASC/TMS1/Pycard using anti-ASC/TMS1/Pycard antibody (A00362-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/Pycard antigen affinity purified polyclonal antibody (Catalog # A00362-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC/TMS1/Pycard at approximately 22 kDa. The expected band size for ASC/TMS1/Pycard is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-5-pycard-primary-antibodies-if-testing-2.jpgAnti-ASC/TMS1/Pycard Antibody Picoband® IF analysis of ASC/TMS1/Pycard using anti-ASC/TMS1/Pycard antibody (A00362-5) and anti-Tubulin Alpha antibody (M03989-3). ASC/TMS1/Pycard was detected in immunocytochemical section of RM1 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ASC/TMS1/Pycard Antibody (A00362-5) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-5-pycard-primary-antibodies-fcm-testing-3.pngAnti-ASC/TMS1/Pycard Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-ASC/TMS1/Pycard antibody (A00362-5). Overlay histogram showing RAW264.7 cells stained with A00362-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/Pycard Antibody (A00362-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pds5b-picoband-trade-antibody-a04823-1-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04823-1-pds5b-primary-antibodies-wb-testing-1.jpgAnti-PDS5B Antibody Picoband® Western blot analysis of PDS5B using anti-PDS5B antibody (A04823-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDS5B antigen affinity purified polyclonal antibody (Catalog # A04823-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDS5B at approximately 170 kDa. The expected band size for PDS5B is at 165 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04823-1-pds5b-primary-antibodies-fcm-testing-2.pngAnti-PDS5B Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-PDS5B antibody (A04823-1). Overlay histogram showing PC-3 cells stained with A04823-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDS5B Antibody (A04823-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ccr4-picoband-trade-antibody-a00755-4-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00755-4-ccr4-primary-antibodies-wb-testing-1.jpgAnti-CCR4 Antibody Picoband® Western blot analysis of CCR4 using anti-CCR4 antibody (A00755-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR4 antigen affinity purified polyclonal antibody (Catalog # A00755-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR4 at approximately 50 kDa. The expected band size for CCR4 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00755-4-ccr4-primary-antibodies-fcm-testing-2.pngAnti-CCR4 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-CCR4 antibody (A00755-4). Overlay histogram showing HEL cells stained with A00755-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR4 Antibody (A00755-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-dll4-picoband-trade-antibody-a00875-4-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00875-4-dll4-primary-antibodies-wb-testing-1.jpgAnti-DLL4 Antibody Picoband® Western blot analysis of DLL4 using anti-DLL4 antibody (A00875-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL4 antigen affinity purified polyclonal antibody (Catalog # A00875-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL4 at approximately 80 kDa. The expected band size for DLL4 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00875-4-dll4-primary-antibodies-fcm-testing-2.pngAnti-DLL4 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-DLL4 antibody (A00875-4). Overlay histogram showing HepG2 cells stained with A00875-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DLL4 Antibody (A00875-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-plekhg5-picoband-trade-antibody-a08522-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-wb-testing-1.jpgAnti-PLEKHG5 Antibody Picoband® Western blot analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHG5 antigen affinity purified polyclonal antibody (Catalog # A08522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHG5 at approximately 125 kDa. The expected band size for PLEKHG5 is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-ihc-testing-2.jpgAnti-PLEKHG5 Antibody Picoband® IHC analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). PLEKHG5 was detected in a paraffin-embedded section of human non-small-cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHG5 Antibody (A08522) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-ihc-testing-3.jpgAnti-PLEKHG5 Antibody Picoband® IHC analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). PLEKHG5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHG5 Antibody (A08522) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-ihc-testing-4.jpgAnti-PLEKHG5 Antibody Picoband® IHC analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). PLEKHG5 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHG5 Antibody (A08522) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-ihc-testing-5.jpgAnti-PLEKHG5 Antibody Picoband® IHC analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). PLEKHG5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHG5 Antibody (A08522) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-ihc-testing-6.jpgAnti-PLEKHG5 Antibody Picoband® IHC analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). PLEKHG5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHG5 Antibody (A08522) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-ihc-testing-7.jpgAnti-PLEKHG5 Antibody Picoband® IHC analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). PLEKHG5 was detected in a paraffin-embedded section of human poorly differentiated adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHG5 Antibody (A08522) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-if-testing-8.jpgAnti-PLEKHG5 Antibody Picoband® IF analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522) and anti-Tubulin Alpha antibody (M03989-3). PLEKHG5 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLEKHG5 Antibody (A08522) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08522-plekhg5-primary-antibodies-if-testing-9.jpgAnti-PLEKHG5 Antibody Picoband® IF analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). PLEKHG5 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLEKHG5 Antibody (A08522) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-plvap-picoband-trade-antibody-a07024-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07024-plvap-primary-antibodies-wb-testing-1.jpgAnti-PLVAP Antibody Picoband® Western blot analysis of PLVAP using anti-PLVAP antibody (A07024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLVAP antigen affinity purified polyclonal antibody (Catalog # A07024) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLVAP at approximately 53 kDa. The expected band size for PLVAP is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07024-plvap-primary-antibodies-fcm-testing-2.pngAnti-PLVAP Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PLVAP antibody (A07024). Overlay histogram showing HEL cells stained with A07024 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLVAP Antibody (A07024, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-plxdc2-picoband-trade-antibody-a08798-1-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08798-1-plxdc2-primary-antibodies-wb-testing-1.jpgAnti-PLXDC2 Antibody Picoband® Western blot analysis of PLXDC2 using anti-PLXDC2 antibody (A08798-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXDC2 antigen affinity purified polyclonal antibody (Catalog # A08798-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLXDC2 at approximately 60 kDa. The expected band size for PLXDC2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08798-1-plxdc2-primary-antibodies-ihc-testing-2.jpgAnti-PLXDC2 Antibody Picoband® IHC analysis of PLXDC2 using anti-PLXDC2 antibody (A08798-1). PLXDC2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLXDC2 Antibody (A08798-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08798-1-plxdc2-primary-antibodies-ihc-testing-3.jpgAnti-PLXDC2 Antibody Picoband® IHC analysis of PLXDC2 using anti-PLXDC2 antibody (A08798-1). PLXDC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLXDC2 Antibody (A08798-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08798-1-plxdc2-primary-antibodies-ihc-testing-4.jpgAnti-PLXDC2 Antibody Picoband® IHC analysis of PLXDC2 using anti-PLXDC2 antibody (A08798-1). PLXDC2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLXDC2 Antibody (A08798-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08798-1-plxdc2-primary-antibodies-fcm-testing-5.pngAnti-PLXDC2 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-PLXDC2 antibody (A08798-1). Overlay histogram showing Caco-2 cells stained with A08798-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLXDC2 Antibody (A08798-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08798-1-plxdc2-primary-antibodies-fcm-testing-6.pngAnti-PLXDC2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PLXDC2 antibody (A08798-1). Overlay histogram showing MCF-7 cells stained with A08798-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLXDC2 Antibody (A08798-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-aplp1-picoband-trade-antibody-a03929-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03929-aplp1-primary-antibodies-wb-testing-1.jpgAnti-APLP1 Antibody Picoband® Western blot analysis of APLP1 using anti-APLP1 antibody (A03929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP1 antigen affinity purified polyclonal antibody (Catalog # A03929) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APLP1 at approximately 72 kDa. The expected band size for APLP1 is at 72,97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03929-aplp1-primary-antibodies-fcm-testing-2.pngAnti-APLP1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-APLP1 antibody (A03929). Overlay histogram showing SiHa cells stained with A03929 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APLP1 Antibody (A03929, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-phtf1-picoband-trade-antibody-a12488-boster.html2026-03-17T05:14:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12488-phtf1-primary-antibodies-wb-testing-1.jpgAnti-PHTF1 Antibody Picoband® Western blot analysis of PHTF1 using anti-PHTF1 antibody (A12488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHTF1 antigen affinity purified polyclonal antibody (Catalog # A12488) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHTF1 at approximately 100 kDa. The expected band size for PHTF1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12488-phtf1-primary-antibodies-fcm-testing-2.pngAnti-PHTF1 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-PHTF1 antibody (A12488). Overlay histogram showing U87 cells stained with A12488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHTF1 Antibody (A12488, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pnpla6-picoband-trade-antibody-a04071-2-boster.html2026-04-06T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04071-2-pnpla6-primary-antibodies-wb-testing-1.jpgAnti-PNPLA6 Antibody Picoband® Western blot analysis of PNPLA6 using anti-PNPLA6 antibody (A04071-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNPLA6 antigen affinity purified polyclonal antibody (Catalog # A04071-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNPLA6 at approximately 150 kDa. The expected band size for PNPLA6 is at 150,151 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04071-2-pnpla6-primary-antibodies-ihc-testing-1.jpgAnti-PNPLA6 Antibody Picoband®IHC analysis of PNPLA6 using anti-PNPLA6 antibody (A04071-2). PNPLA6 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PNPLA6 Antibody (A04071-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04071-2-pnpla6-primary-antibodies-if-testing-2.jpgAnti-PNPLA6 Antibody Picoband® IF analysis of PNPLA6 using anti-PNPLA6 antibody (A04071-2). PNPLA6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PNPLA6 Antibody (A04071-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04071-2-pnpla6-primary-antibodies-fcm-testing-3.pngAnti-PNPLA6 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PNPLA6 antibody (A04071-2). Overlay histogram showing SiHa cells stained with A04071-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNPLA6 Antibody (A04071-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-connexin-32-gjb1-picoband-trade-antibody-a01050-1-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01050-gjb1-primary-antibodies-wb-testing-1.jpgAnti-Connexin-32/GJB1 Antibody Picoband® Western blot analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (A01050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin-32/GJB1 antigen affinity purified polyclonal antibody (Catalog # A01050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin-32/GJB1 at approximately 35 kDa. The expected band size for Connexin-32/GJB1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01050-gjb1-primary-antibodies-fcm-testing-2.pngAnti-Connexin-32/GJB1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Connexin-32/GJB1 antibody (A01050). Overlay histogram showing HepG2 cells stained with A01050 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Connexin-32/GJB1 Antibody (A01050, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-dr5-tnfrsf10b-picoband-trade-antibody-a00410-5-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-5-tnfrsf10b-primary-antibodies-wb-testing-1.jpgAnti-DR5/TNFRSF10B Antibody Picoband® Western blot analysis of DR5/TNFRSF10B using anti-DR5/TNFRSF10B antibody (A00410-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR5/TNFRSF10B antigen affinity purified polyclonal antibody (Catalog # A00410-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR5/TNFRSF10B at approximately 45 kDa. The expected band size for DR5/TNFRSF10B is at 32,45-50,58-60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-5-tnfrsf10b-primary-antibodies-ihc-testing-2.jpgAnti-DR5/TNFRSF10B Antibody Picoband® IHC analysis of DR5/TNFRSF10B using anti-DR5/TNFRSF10B antibody (A00410-5). DR5/TNFRSF10B was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DR5/TNFRSF10B Antibody (A00410-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-5-tnfrsf10b-primary-antibodies-ihc-testing-3.jpgAnti-DR5/TNFRSF10B Antibody Picoband® IHC analysis of DR5/TNFRSF10B using anti-DR5/TNFRSF10B antibody (A00410-5). DR5/TNFRSF10B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DR5/TNFRSF10B Antibody (A00410-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-5-tnfrsf10b-primary-antibodies-ihc-testing-4.jpgAnti-DR5/TNFRSF10B Antibody Picoband® IHC analysis of DR5/TNFRSF10B using anti-DR5/TNFRSF10B antibody (A00410-5). DR5/TNFRSF10B was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DR5/TNFRSF10B Antibody (A00410-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00410-5-tnfrsf10b-primary-antibodies-fcm-testing-5.pngAnti-DR5/TNFRSF10B Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-DR5/TNFRSF10B antibody (A00410-5). Overlay histogram showing 293T cells stained with A00410-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DR5/TNFRSF10B Antibody (A00410-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-hprt1-picoband-trade-antibody-a00668-1-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00668-1-hprt1-primary-antibodies-wb-testing-1_1.jpgAnti-HPRT1 Antibody Picoband®Western blot analysis of HPRT1 using anti-HPRT1 antibody (A00668-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT1 antigen affinity purified polyclonal antibody (A00668-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HPRT1 at approximately 25 kDa. The expected band size for HPRT1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00668-1-hprt1-primary-antibodies-ihc-testing-1.jpgAnti-HPRT1 Antibody Picoband®IHC analysis of HPRT1 using anti-HPRT1 antibody (A00668-1). HPRT1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (A00668-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00668-1-hprt1-primary-antibodies-if-testing-1.jpgAnti-HPRT1 Antibody Picoband®IF analysis of HPRT1 using anti-HPRT1 antibody (A00668-1). HPRT1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HPRT1 Antibody (A00668-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00668-1-hprt1-primary-antibodies-fcm-testing-1.jpgAnti-HPRT1 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-HPRT1 antibody (A00668-1). Overlay histogram showing A549 cells stained with A00668-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HPRT1 Antibody (A00668-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-cea-ceacam5-picoband-trade-antibody-a00356-2-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-2-ceacam5-primary-antibodies-wb-testing-1.jpgAnti-CEA/CEACAM5 Antibody Picoband® Western blot analysis of CEA/CEACAM5 using anti-CEA/CEACAM5 antibody (A00356-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA/CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA/CEACAM5 at approximately 200 kDa. The expected band size for CEA/CEACAM5 is at 77,180-200 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00356-2-ceacam5-primary-antibodies-fcm-testing-2.pngAnti-CEA/CEACAM5 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-CEA/CEACAM5 antibody (A00356-2). Overlay histogram showing PC-3 cells stained with A00356-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CEA/CEACAM5 Antibody (A00356-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-foxo3a-foxo3-picoband-trade-antibody-a00252-2-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00252-2-foxo3-primary-antibodies-wb-testing-1.jpgAnti-FOXO3A/FOXO3 Antibody Picoband® Western blot analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (A00252-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO3A/FOXO3 antigen affinity purified polyclonal antibody (Catalog # A00252-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO3A/FOXO3 at approximately 90 kDa. The expected band size for FOXO3A/FOXO3 is at 71,80-100 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-mmp2-picoband-trade-antibody-a00286-3-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00286-3-mmp2-primary-antibodies-wb-testing-1.jpgAnti-MMP2 Antibody Picoband® Western blot analysis of MMP2 using anti-MMP2 antibody (A00286-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP2 antigen affinity purified polyclonal antibody (Catalog # A00286-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP2 at approximately 70 kDa. The expected band size for MMP2 is at 64,66,70,72,74 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cd326-epcam-picoband-trade-antibody-a00276-2-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00276-2-epcam-primary-antibodies-wb-testing-1.jpgAnti-CD326/Epcam Antibody Picoband® Western blot analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse small intestine tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD326/Epcam antigen affinity purified polyclonal antibody (Catalog # A00276-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD326/Epcam at approximately 38 kDa. The expected band size for CD326/Epcam is at 35,40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00276-2-epcam-primary-antibodies-ihc-testing-2.jpgAnti-CD326/Epcam Antibody Picoband® IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2). CD326/Epcam was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD326/Epcam Antibody (A00276-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00276-2-epcam-primary-antibodies-ihc-testing-3.jpgAnti-CD326/Epcam Antibody Picoband® IHC analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2). CD326/Epcam was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD326/Epcam Antibody (A00276-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00276-2-epcam-primary-antibodies-if-testing-4.jpgAnti-CD326/Epcam Antibody Picoband® IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2). CD326/Epcam was detected in a paraffin-embedded section of mouse colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD326/Epcam Antibody (A00276-2) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00276-2-epcam-primary-antibodies-if-testing-5.jpgAnti-CD326/Epcam Antibody Picoband® IF analysis of CD326/Epcam using anti-CD326/Epcam antibody (A00276-2). CD326/Epcam was detected in a paraffin-embedded section of rat colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD326/Epcam Antibody (A00276-2) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00276-2-epcam-primary-antibodies-fcm-testing-6.pngAnti-CD326/Epcam Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-CD326/Epcam antibody (A00276-2). Overlay histogram showing RAW264.7 cells stained with A00276-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD326/Epcam Antibody (A00276-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-atf4-picoband-trade-antibody-a00371-4-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00371-4-atf4-primary-antibodies-wb-testing-1.jpgAnti-ATF4 Antibody Picoband® Western blot analysis of ATF4 using anti-ATF4 antibody (A00371-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (Catalog # A00371-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 45-50 kDa. The expected band size for ATF4 is at 38,39,45-50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00371-4-atf4-primary-antibodies-if-testing-2.jpgAnti-ATF4 Antibody Picoband® IF analysis of ATF4 using anti-ATF4 antibody (A00371-4) and anti-Tubulin Alpha antibody (M03989-3). ATF4 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATF4 Antibody (A00371-4) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00371-4-atf4-primary-antibodies-fcm-testing-3.pngAnti-ATF4 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-ATF4 antibody (A00371-4). Overlay histogram showing SiHa cells stained with A00371-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF4 Antibody (A00371-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-cd3-gamma-cd3g-picoband-trade-antibody-a04853-2-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04853-2-cd3g-primary-antibodies-wb-testing-1.jpgAnti-CD3 Gamma/CD3G Antibody Picoband® Western blot analysis of CD3 Gamma/CD3G using anti-CD3 Gamma/CD3G antibody (A04853-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOMLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 Gamma/CD3G antigen affinity purified polyclonal antibody (Catalog # A04853-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 Gamma/CD3G at approximately 20-25 kDa. The expected band size for CD3 Gamma/CD3G is at 20-25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04853-2-cd3g-primary-antibodies-ihc-testing-1.jpgAnti-CD3 Gamma/CD3G Antibody Picoband®IHC analysis of CD3 Gamma/CD3G using anti-CD3 Gamma/CD3G antibody (A04853-2). CD3 Gamma/CD3G was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD3 Gamma/CD3G Antibody (A04853-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04853-2-cd3g-primary-antibodies-if-testing-2.jpgAnti-CD3 Gamma/CD3G Antibody Picoband® IF analysis of CD3 Gamma/CD3G using anti-CD3 Gamma/CD3G antibody (A04853-2). CD3 Gamma/CD3G was detected in an immunocytochemical section of JK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CD3 Gamma/CD3G Antibody (A04853-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04853-2-cd3g-primary-antibodies-fcm-testing-3.pngAnti-CD3 Gamma/CD3G Antibody Picoband® Flow Cytometry analysis of JK cells using anti-CD3 Gamma/CD3G antibody (A04853-2). Overlay histogram showing JK cells stained with A04853-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD3 Gamma/CD3G Antibody (A04853-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-tm2d3-picoband-trade-antibody-a15600-1-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15600-1-tm2d3-primary-antibodies-wb-testing-1.jpgAnti-TM2D3 Antibody Picoband® Western blot analysis of TM2D3 using anti-TM2D3 antibody (A15600-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM2D3 antigen affinity purified polyclonal antibody (Catalog # A15600-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM2D3 at approximately 37 kDa. The expected band size for TM2D3 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15600-1-tm2d3-primary-antibodies-if-testing-2.jpgAnti-TM2D3 Antibody Picoband® IF analysis of TM2D3 using anti-TM2D3 antibody (A15600-1) and anti-Tubulin Alpha antibody (M03989-3). TM2D3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TM2D3 Antibody (A15600-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15600-1-tm2d3-primary-antibodies-fcm-testing-3.pngAnti-TM2D3 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-TM2D3 antibody (A15600-1). Overlay histogram showing HepG2 cells stained with A15600-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TM2D3 Antibody (A15600-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nfe2-picoband-trade-antibody-a03765-1-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03765-1-nfe2-primary-antibodies-wb-testing-1.jpgAnti-NFE2 Antibody Picoband® Western blot analysis of NFE2 using anti-NFE2 antibody (A03765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFE2 antigen affinity purified polyclonal antibody (Catalog # A03765-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFE2 at approximately 41 kDa. The expected band size for NFE2 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-fibrillin-2-fbn2-picoband-trade-antibody-a03408-2-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03408-2-fbn2-primary-antibodies-wb-testing-1.jpgAnti-fibrillin 2/FBN2 Antibody Picoband® Western blot analysis of Fibrillin 2/FBN2 using anti-Fibrillin 2/FBN2 antibody (A03408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillin 2/FBN2 antigen affinity purified polyclonal antibody (Catalog # A03408-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillin 2/FBN2 at approximately 315,250 kDa. The expected band size for Fibrillin 2/FBN2 is at 315 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-mcl1-picoband-trade-antibody-a00712-2-boster.html2026-03-17T05:14:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00712-2-mcl1-primary-antibodies-wb-testing-1.jpgAnti-MCL1 Antibody Picoband® Western blot analysis of MCL1 using anti-MCL1 antibody (A00712-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat kisney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCL1 antigen affinity purified polyclonal antibody (Catalog # A00712-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCL1 at approximately 40 kDa. The expected band size for MCL1 is at 28,37,40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00712-2-mcl1-primary-antibodies-if-testing-2.jpgAnti-MCL1 Antibody Picoband® IF analysis of MCL1 using anti-MCL1 antibody (A00712-2). MCL1 was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MCL1 Antibody (A00712-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00712-2-mcl1-primary-antibodies-fcm-testing-3.pngAnti-MCL1 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-MCL1 antibody (A00712-2). Overlay histogram showing RT4 cells stained with A00712-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCL1 Antibody (A00712-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-gpx1-picoband-trade-antibody-a01019-2-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01019-2-gpx1-primary-antibodies-wb-testing-1.jpgAnti-GPX1 Antibody Picoband® Western blot analysis of GPX1 using anti-GPX1 antibody (A01019-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 antigen affinity purified polyclonal antibody (Catalog # A01019-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22 kDa. The expected band size for GPX1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01019-2-gpx1-primary-antibodies-ihc-testing-2.jpgAnti-GPX1 Antibody Picoband® IHC analysis of GPX1 using anti-GPX1 antibody (A01019-2). GPX1 was detected in a paraffin-embedded section of human prostatic acinar adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPX1 Antibody (A01019-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01019-2-gpx1-primary-antibodies-ihc-testing-3.jpgAnti-GPX1 Antibody Picoband® IHC analysis of GPX1 using anti-GPX1 antibody (A01019-2). GPX1 was detected in a paraffin-embedded section of human cervica squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPX1 Antibody (A01019-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01019-2-gpx1-primary-antibodies-ihc-testing-4.jpgAnti-GPX1 Antibody Picoband® IHC analysis of GPX1 using anti-GPX1 antibody (A01019-2). GPX1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPX1 Antibody (A01019-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-stradb-picoband-trade-antibody-a05271-2-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05271-2-stradb-primary-antibodies-wb-testing-1.jpgAnti-STRADB Antibody Picoband® Western blot analysis of STRADB using anti-STRADB antibody (A05271-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STRADB antigen affinity purified polyclonal antibody (Catalog # A05271-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STRADB at approximately 33 kDa. The expected band size for STRADB is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05271-2-stradb-primary-antibodies-if-testing-2.jpgAnti-STRADB Antibody Picoband® IF analysis of STRADB using anti-STRADB antibody (A05271-2). STRADB was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STRADB Antibody (A05271-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05271-2-stradb-primary-antibodies-fcm-testing-3.pngAnti-STRADB Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-STRADB antibody (A05271-2). Overlay histogram showing SiHa cells stained with A05271-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STRADB Antibody (A05271-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-tlr2-picoband-trade-antibody-a00131-4-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00131-4-tlr2-primary-antibodies-wb-testing-1.jpgAnti-Tlr2 Antibody Picoband® Western blot analysis of Tlr2 using anti-Tlr2 antibody (A00131-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr2 antigen affinity purified polyclonal antibody (Catalog # A00131-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr2 at approximately 89 kDa. The expected band size for Tlr2 is at 89 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ppan-picoband-trade-antibody-a10836-1-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10836-1-ppan-primary-antibodies-wb-testing-1.jpgAnti-PPAN Antibody Picoband® Western blot analysis of PPAN using anti-PPAN antibody (A10836-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAN antigen affinity purified polyclonal antibody (Catalog # A10836-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAN at approximately 65 kDa. The expected band size for PPAN is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10836-1-ppan-primary-antibodies-if-testing-2.jpgAnti-PPAN Antibody Picoband® IF analysis of PPAN using anti-PPAN antibody (A10836-1). PPAN was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPAN Antibody (A10836-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10836-1-ppan-primary-antibodies-fcm-testing-3.pngAnti-PPAN Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PPAN antibody (A10836-1). Overlay histogram showing JK cells stained with A10836-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAN Antibody (A10836-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-polr2h-picoband-trade-antibody-a11270-1-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11270-1-polr2h-primary-antibodies-wb-testing-1_1.jpgAnti-POLR2H Antibody Picoband®Western blot analysis of POLR2H using anti-POLR2H antibody (A11270-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. Lane 3: human MCF-7 whole cell lysates. Lane 4: rat liver tissue lysates. Lane 5: rat RH35 whole cell lysates. Lane 6: mouse liver tissue lysates. Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2H antigen affinity purified polyclonal antibody (A11270-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLR2H at approximately 17 kDa. The expected band size for POLR2H is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11270-1-polr2h-primary-antibodies-ihc-testing-1.jpgAnti-POLR2H Antibody Picoband®IHC analysis of POLR2H using anti-POLR2H antibody (A11270-1). POLR2H was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLR2H Antibody (A11270-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11270-1-polr2h-primary-antibodies-if-testing-1.jpgAnti-POLR2H Antibody Picoband®IF analysis of POLR2H using anti-POLR2H antibody (A11270-1) and anti-Alpha Tubulin antibody (M03989-3). POLR2H was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POLR2H Antibody (A11270-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11270-1-polr2h-primary-antibodies-if-testing-2_1.jpgAnti-POLR2H Antibody Picoband®IF analysis of POLR2H using anti-POLR2H antibody (A11270-1). POLR2H was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-POLR2H Antibody (A11270-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11270-1-polr2h-primary-antibodies-ip-testing-1_1.jpgAnti-POLR2H Antibody Picoband®Immunoprecipitating (IP) POLR2H in MCF-7 whole cell lysate. Western blot analysis of POLR2H using anti-POLR2H antibody (A11270-1); Lane 1: MCF-7 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-POLR2H antibody in MCF-7 whole cell lysate; Lane 3: anti-POLR2H antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR2H antigen affinity purified polyclonal antibody (A11270-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for POLR2H at approximately 17 kDa. The expected band size for POLR2H is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-angiogenin-ang-picoband-trade-antibody-a00146-3-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00146-3-ang-primary-antibodies-wb-testing-1.jpgAnti-Angiogenin/Ang Antibody Picoband® Western blot analysis of Angiogenin/Ang using anti-Angiogenin/Ang antibody (A00146-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiogenin/Ang antigen affinity purified polyclonal antibody (Catalog # A00146-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiogenin/Ang at approximately 25 kDa. The expected band size for Angiogenin/Ang is at 17-22 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pdxp-picoband-trade-antibody-a09629-2-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09629-2-pdxp-primary-antibodies-wb-testing-1.jpgAnti-PDXP Antibody Picoband® Western blot analysis of PDXP using anti-PDXP antibody (A09629-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXP antigen affinity purified polyclonal antibody (Catalog # A09629-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDXP at approximately 37 kDa. The expected band size for PDXP is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09629-2-pdxp-primary-antibodies-fcm-testing-2.pngAnti-PDXP Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PDXP antibody (A09629-2). Overlay histogram showing HEL cells stained with A09629-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDXP Antibody (A09629-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-plekha1-picoband-trade-antibody-a08988-1-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-wb-testing-1.jpgAnti-PLEKHA1 Antibody Picoband® Western blot analysis of PLEKHA1 using anti-PLEKHA1 antibody (A08988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHA1 antigen affinity purified polyclonal antibody (Catalog # A08988-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHA1 at approximately 46 kDa. The expected band size for PLEKHA1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-ihc-testing-2.jpgAnti-PLEKHA1 Antibody Picoband® IHC analysis of PLEKHA1 using anti-PLEKHA1 antibody (A08988-1). PLEKHA1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHA1 Antibody (A08988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-ihc-testing-3.jpgAnti-PLEKHA1 Antibody Picoband® IHC analysis of PLEKHA1 using anti-PLEKHA1 antibody (A08988-1). PLEKHA1 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHA1 Antibody (A08988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-ihc-testing-4.jpgAnti-PLEKHA1 Antibody Picoband® IHC analysis of PLEKHA1 using anti-PLEKHA1 antibody (A08988-1). PLEKHA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHA1 Antibody (A08988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-ihc-testing-5.jpgAnti-PLEKHA1 Antibody Picoband® IHC analysis of PLEKHA1 using anti-PLEKHA1 antibody (A08988-1). PLEKHA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLEKHA1 Antibody (A08988-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-if-testing-6.jpgAnti-PLEKHA1 Antibody Picoband® IF analysis of PLEKHA1 using anti-PLEKHA1 antibody (A08988-1). PLEKHA1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLEKHA1 Antibody (A08988-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-fcm-testing-7.pngAnti-PLEKHA1 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PLEKHA1 antibody (A08988-1). Overlay histogram showing A431 cells stained with A08988-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLEKHA1 Antibody (A08988-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08988-1-plekha1-primary-antibodies-if-testing-8.jpgAnti-PLEKHA1 Antibody Picoband® IF analysis of PLEKHA1 using anti-PLEKHA1 antibody (A08988-1). PLEKHA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLEKHA1 Antibody (A08988-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ppm1g-picoband-trade-antibody-a06725-2-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-wb-testing-1_1.jpgAnti-PPM1G Antibody Picoband® Western blot analysis of PPM1G using anti-PPM1G antibody (A06725-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1G antigen affinity purified polyclonal antibody (Catalog # A06725-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPM1G at approximately 75 kDa. The expected band size for PPM1G is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-ihc-testing-2.jpgAnti-PPM1G Antibody Picoband® IHC analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-ihc-testing-3.jpgAnti-PPM1G Antibody Picoband® IHC analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-ihc-testing-4.jpgAnti-PPM1G Antibody Picoband® IHC analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-ihc-testing-5.jpgAnti-PPM1G Antibody Picoband® IHC analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human lymphoma cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-ihc-testing-6.jpgAnti-PPM1G Antibody Picoband® IHC analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human skin squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-ihc-testing-7.jpgAnti-PPM1G Antibody Picoband® IHC analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-if-testing-8.jpgAnti-PPM1G Antibody Picoband® IF analysis of PPM1G using anti-PPM1G antibody (A06725-2) and anti-Tubulin Alpha antibody (M03989-3). PPM1G was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPM1G Antibody (A06725-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-if-testing-9.jpgAnti-PPM1G Antibody Picoband®IF analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human pancreas squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-if-testing-10.jpgAnti-PPM1G Antibody Picoband® IF analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-if-testing-11.jpgAnti-PPM1G Antibody Picoband® IF analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-if-testing-12.jpgAnti-PPM1G Antibody Picoband® IF analysis of PPM1G using anti-PPM1G antibody (A06725-2). PPM1G was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PPM1G Antibody (A06725-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-ip-testing-13.jpgAnti-PPM1G Antibody Picoband® Immunoprecipitating (IP) PPM1G in Hela whole cell lysate. Western blot analysis of PPM1G using anti-PPM1G antibody (A06725-2); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PPM1G antibody in Hela whole cell lysate; Lane 3: anti-PPM1G antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPM1G antigen affinity purified polyclonal antibody (A06725-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PPM1G at approximately 75 kDa. The expected band size for PPM1G is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06725-2-ppm1g-primary-antibodies-fcm-testing-1.jpgAnti-PPM1G Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-PPM1G antibody (A06725-2). Overlay histogram showing MCF-7 cells stained with A06725-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1G Antibody (A06725-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-prdm15-picoband-trade-antibody-a13548-1-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-wb-testing-1.jpgAnti-PRDM15 Antibody Picoband® Western blot analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM15 antigen affinity purified polyclonal antibody (Catalog # A13548-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM15 at approximately 169 kDa. The expected band size for PRDM15 is at 168,62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-ihc-testing-2.jpgAnti-PRDM15 Antibody Picoband® IHC analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). PRDM15 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM15 Antibody (A13548-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-ihc-testing-3.jpgAnti-PRDM15 Antibody Picoband® IHC analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). PRDM15 was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM15 Antibody (A13548-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-ihc-testing-4.jpgAnti-PRDM15 Antibody Picoband® IHC analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). PRDM15 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM15 Antibody (A13548-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-ihc-testing-5.jpgAnti-PRDM15 Antibody Picoband® IHC analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). PRDM15 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM15 Antibody (A13548-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-if-testing-6.jpgAnti-PRDM15 Antibody Picoband® IF analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). PRDM15 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PRDM15 Antibody (A13548-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-if-testing-7.jpgAnti-PRDM15 Antibody Picoband® IF analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). PRDM15 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PRDM15 Antibody (A13548-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13548-1-prdm15-primary-antibodies-fcm-testing-8.pngAnti-PRDM15 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PRDM15 antibody (A13548-1). Overlay histogram showing A431 cells stained with A13548-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM15 Antibody (A13548-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pgk2-picoband-trade-antibody-a08946-1-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08946-1-pgk2-primary-antibodies-wb-testing-1.jpgAnti-PGK2 Antibody Picoband® Western blot analysis of PGK2 using anti-PGK2 antibody (A08946-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGK2 antigen affinity purified polyclonal antibody (Catalog # A08946-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGK2 at approximately 45 kDa. The expected band size for PGK2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08946-1-pgk2-primary-antibodies-if-testing-2.jpgAnti-PGK2 Antibody Picoband® IF analysis of PGK2 using anti-PGK2 antibody (A08946-1). PGK2 was detected in an immunocytochemical section of RM1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PGK2 Antibody (A08946-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08946-1-pgk2-primary-antibodies-fcm-testing-3.pngAnti-PGK2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PGK2 antibody (A08946). Overlay histogram showing SiHa cells stained with A08946 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGK2 Antibody (A08946, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08946-1-pgk2-primary-antibodies-fcm-testing-4.pngAnti-PGK2 Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-PGK2 antibody (A08946). Overlay histogram showing U20S cells stained with A08946 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGK2 Antibody (A08946, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pmis2-picoband-trade-antibody-a31792-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31792-pmis2-primary-antibodies-wb-testing-1.jpgAnti-PMIS2 Antibody Picoband® Western blot analysis of PMIS2 using anti-PMIS2 antibody (A31792). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMIS2 antigen affinity purified polyclonal antibody (Catalog # A31792) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PMIS2 at approximately 19 kDa. The expected band size for PMIS2 is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cdc42-picoband-trade-antibody-a00119-1-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-1-cdc42-primary-antibodies-wb-testing-1.jpgAnti-CDC42 Antibody Picoband® Western blot analysis of CDC42 using anti-CDC42 antibody (A00119-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 antigen affinity purified polyclonal antibody (Catalog # A00119-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC42 at approximately 21 kDa. The expected band size for CDC42 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-1-cdc42-primary-antibodies-if-testing-2.jpgAnti-CDC42 Antibody Picoband® IF analysis of CDC42 using anti-CDC42 antibody (A00119-1). CDC42 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CDC42 Antibody (A00119-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00119-1-cdc42-primary-antibodies-fcm-testing-3.pngAnti-CDC42 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-CDC42 antibody (A00119-1). Overlay histogram showing MCF-7 cells stained with A00119-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC42 Antibody (A00119-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ldlr-picoband-trade-antibody-a00076-3-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00076-3-ldlr-primary-antibodies-wb-testing-1.jpgAnti-LDLR Antibody Picoband® Western blot analysis of LDLR using anti-LDLR antibody (A00076-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDLR antigen affinity purified polyclonal antibody (Catalog # A00076-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDLR at approximately 140-160 kDa. The expected band size for LDLR is at 95,140-160,120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00076-3-ldlr-primary-antibodies-fcm-testing-2.pngAnti-LDLR Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-LDLR antibody (A00076-3). Overlay histogram showing MCF-7 cells stained with A00076-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDLR Antibody (A00076-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ager-picoband-trade-antibody-a03438-2-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03438-2-ager-primary-antibodies-wb-testing-1.jpgAnti-Ager Antibody Picoband® Western blot analysis of Ager using anti-Ager antibody (A03438-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ager antigen affinity purified polyclonal antibody (Catalog # A03438-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ager at approximately 43,58 kDa. The expected band size for Ager is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-polr1b-picoband-trade-antibody-a11908-2-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11908-2-polr1b-primary-antibodies-wb-testing-1.jpgAnti-POLR1B Antibody Picoband® Western blot analysis of POLR1B using anti-POLR1B antibody (A11908-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1B antigen affinity purified polyclonal antibody (Catalog # A11908-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1B at approximately 128 kDa. The expected band size for POLR1B is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11908-2-polr1b-primary-antibodies-ihc-testing-2.jpgAnti-POLR1B Antibody Picoband® IHC analysis of POLR1B using anti-POLR1B antibody (A11908-2). POLR1B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLR1B Antibody (A11908-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11908-2-polr1b-primary-antibodies-ihc-testing-3.jpgAnti-POLR1B Antibody Picoband® IHC analysis of POLR1B using anti-POLR1B antibody (A11908-2). POLR1B was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLR1B Antibody (A11908-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11908-2-polr1b-primary-antibodies-if-testing-4.jpgAnti-POLR1B Antibody Picoband® IF analysis of POLR1B using anti-POLR1B antibody (A11908-2). POLR1B was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POLR1B Antibody (A11908-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11908-2-polr1b-primary-antibodies-fcm-testing-5.pngAnti-POLR1B Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-POLR1B antibody (A11908-2). Overlay histogram showing A431 cells stained with A11908-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLR1B Antibody (A11908-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-polr1c-picoband-trade-antibody-a07025-2-boster.html2026-03-17T05:14:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07025-2-polr1c-primary-antibodies-wb-testing-1.jpgAnti-POLR1C Antibody Picoband® Western blot analysis of POLR1C using anti-POLR1C antibody (A07025-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1C antigen affinity purified polyclonal antibody (Catalog # A07025-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1C at approximately 40 kDa. The expected band size for POLR1C is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07025-2-polr1c-primary-antibodies-fcm-testing-2.pngAnti-POLR1C Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-POLR1C antibody (A07025-2). Overlay histogram showing RT4 cells stained with A07025-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLR1C Antibody (A07025-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-bcl11b-picoband-trade-antibody-a01485-2-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-wb-testing-1.jpgAnti-BCL11B Antibody Picoband® Western blot analysis of BCL11B using anti-BCL11B antibody (A01485-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL11B antigen affinity purified polyclonal antibody (Catalog # A01485-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL11B at approximately 116 kDa. The expected band size for BCL11B is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-2.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human bladder urothelial carcinoma with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-3.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-4.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-5.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-6.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human lymph node metastasis carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-7.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-8.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-ihc-testing-9.jpgAnti-BCL11B Antibody Picoband® IHC analysis of BCL11B using anti-BCL11B antibody (A01485-2). BCL11B was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL11B Antibody (A01485-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-if-testing-10.jpgAnti-BCL11B Antibody Picoband® IF analysis of BCL11B using anti-BCL11B antibody (A01485-2) and anti-Tubulin Alpha antibody (M03989-3). BCL11B was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BCL11B Antibody (A01485-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01485-2-bcl11b-primary-antibodies-fcm-testing-11.pngAnti-BCL11B Antibody Picoband® Flow Cytometry analysis of C6 cells using anti-BCL11B antibody (A01485-2). Overlay histogram showing C6 cells stained with A01485-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL11B Antibody (A01485-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp1r9a-picoband-trade-antibody-a07331-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07331-1-ppp1r9a-primary-antibodies-wb-testing-1.jpgAnti-PPP1R9A Antibody Picoband® Western blot analysis of PPP1R9A using anti-PPP1R9A antibody (A07331-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R9A antigen affinity purified polyclonal antibody (Catalog # A07331-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R9A at approximately 190 kDa. The expected band size for PPP1R9A is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07331-1-ppp1r9a-primary-antibodies-fcm-testing-2.pngAnti-PPP1R9A Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PPP1R9A antibody (A07331-1). Overlay histogram showing MCF-7 cells stained with A07331-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R9A Antibody (A07331-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp2r5b-picoband-trade-antibody-a09894-2-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09894-2-ppp2r5b-primary-antibodies-wb-testing-1.jpgAnti-PPP2R5B Antibody Picoband® Western blot analysis of PPP2R5B using anti-PPP2R5B antibody (A09894-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5B antigen affinity purified polyclonal antibody (Catalog # A09894-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R5B at approximately 65 kDa. The expected band size for PPP2R5B is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09894-2-ppp2r5b-primary-antibodies-fcm-testing-2.pngAnti-PPP2R5B Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PPP2R5B antibody (A09894-2). Overlay histogram showing SiHa cells stained with A09894-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R5B Antibody (A09894-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp2r5d-picoband-trade-antibody-a06450-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06450-1-ppp2r5d-primary-antibodies-wb-testing-1_1.jpgAnti-PPP2R5D Antibody Picoband® Western blot analysis of PPP2R5D using anti-PPP2R5D antibody (A06450-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5D antigen affinity purified polyclonal antibody (A06450-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP2R5D at approximately 74 kDa. The expected band size for PPP2R5D is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06450-1-ppp2r5d-primary-antibodies-ihc-testing-2.jpgAnti-PPP2R5D Antibody Picoband® IHC analysis of PPP2R5D using anti-PPP2R5D antibody (A06450-1). PPP2R5D was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R5D Antibody (A06450-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06450-1-ppp2r5d-primary-antibodies-if-testing-3.jpgAnti-PPP2R5D Antibody Picoband® IF analysis of PPP2R5D using anti-PPP2R5D antibody (A06450-1) and anti-Tubulin Alpha antibody (M03989-3). PPP2R5D was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP2R5D Antibody (A06450-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06450-1-ppp2r5d-primary-antibodies-ip-testing-4.jpgAnti-PPP2R5D Antibody Picoband® Immunoprecipitating PPP2R5D in HepG2 whole cell lysate. Western blot analysis of PPP2R5D using anti-PPP2R5D antibody (A06450-1); Lane 1: HepG2 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PPP2R5D antibody in HepG2 whole cell lysate; Lane 3: anti-PPP2R5D antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PPP2R5D antigen affinity purified polyclonal antibody (A06450-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PPP2R5D at approximately 74 kDa. The expected band size for PPP2R5D is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06450-1-ppp2r5d-primary-antibodies-fcm-testing-5.jpgAnti-PPP2R5D Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PPP2R5D antibody (A06450-1). Overlay histogram showing 293T cells stained with A06450-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R5D Antibody (A06450-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp3ca-picoband-trade-antibody-a03026-3-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03026-3-ppp3ca-primary-antibodies-wb-testing-1.jpgAnti-PPP3CA Antibody Picoband® Western blot analysis of PPP3CA using anti-PPP3CA antibody (A03026-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CA antigen affinity purified polyclonal antibody (Catalog # A03026-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3CA at approximately 59 kDa. The expected band size for PPP3CA is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03026-3-ppp3ca-primary-antibodies-if-testing-2.jpgAnti-PPP3CA Antibody Picoband® IF analysis of PPP3CA using anti-PPP3CA antibody (A03026-3). PPP3CA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP3CA Antibody (A03026-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03026-3-ppp3ca-primary-antibodies-fcm-testing-3.pngAnti-PPP3CA Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PPP3CA antibody (A03026-3). Overlay histogram showing SiHa cells stained with A03026-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP3CA Antibody (A03026-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp4c-picoband-trade-antibody-a06390-4-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-wb-testing-1.jpgAnti-PPP4C Antibody Picoband® Western blot analysis of PPP4C using anti-PPP4C antibody (A06390-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP4C antigen affinity purified polyclonal antibody (Catalog # A06390-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP4C at approximately 35 kDa. The expected band size for PPP4C is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-ihc-testing-2.jpgAnti-PPP4C Antibody Picoband® IHC analysis of PPP4C using anti-PPP4C antibody (A06390-4). PPP4C was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP4C Antibody (A06390-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-ihc-testing-3.jpgAnti-PPP4C Antibody Picoband® IHC analysis of PPP4C using anti-PPP4C antibody (A06390-4). PPP4C was detected in a paraffin-embedded section of human Invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP4C Antibody (A06390-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-ihc-testing-4.jpgAnti-PPP4C Antibody Picoband® IHC analysis of PPP4C using anti-PPP4C antibody (A06390-4). PPP4C was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP4C Antibody (A06390-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-ihc-testing-5.jpgAnti-PPP4C Antibody Picoband® IHC analysis of PPP4C using anti-PPP4C antibody (A06390-4). PPP4C was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP4C Antibody (A06390-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-ihc-testing-6.jpgAnti-PPP4C Antibody Picoband® IHC analysis of PPP4C using anti-PPP4C antibody (A06390-4). PPP4C was detected in a paraffin-embedded section of human squamous cell carcinoma skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP4C Antibody (A06390-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-if-testing-7.jpgAnti-PPP4C Antibody Picoband® IF analysis of PPP4C using anti-PPP4C antibody (A06390-4) and anti-Tubulin Alpha antibody (M03989-3). PPP4C was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP4C Antibody (A06390-4) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06390-4-ppp4c-primary-antibodies-fcm-testing-8.pngAnti-PPP4C Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PPP4C antibody (A06390-4). Overlay histogram showing MCF-7 cells stained with A06390-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP4C Antibody (A06390-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prdm7-picoband-trade-antibody-a13923-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13923-1-prdm7-primary-antibodies-wb-testing-1.jpgAnti-PRDM7 Antibody Picoband® Western blot analysis of PRDM7 using anti-PRDM7 antibody (A13923-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM7 antigen affinity purified polyclonal antibody (Catalog # A13923-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM7 at approximately 46 kDa. The expected band size for PRDM7 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pkc-alpha-prkca-picoband-trade-antibody-a00743-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00743-1-prkca-primary-antibodies-wb-testing-1.jpgAnti-PKC Alpha/PRKCA Antibody Picoband® Western blot analysis of PKC Alpha/PRKCA using anti-PKC Alpha/PRKCA antibody (A00743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (Catalog # A00743-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00743-1-prkca-primary-antibodies-fcm-testing-2.pngAnti-PKC Alpha/PRKCA Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PKC Alpha/PRKCA antibody (A00743-1). Overlay histogram showing SiHa cells stained with A00743-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC Alpha/PRKCA Antibody (A00743-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prmt1-picoband-trade-antibody-a01417-4-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01417-4-prmt1-primary-antibodies-wb-testing-1.jpgAnti-PRMT1 Antibody Picoband® Western blot analysis of PRMT1 using anti-PRMT1 antibody (A01417-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT1 antigen affinity purified polyclonal antibody (Catalog # A01417-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT1 at approximately 39 kDa. The expected band size for PRMT1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01417-4-prmt1-primary-antibodies-if-testing-2.jpgAnti-PRMT1 Antibody Picoband® IF analysis of PRMT1 using anti-PRMT1 antibody (A01417-4). PRMT1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRMT1 Antibody (A01417-4) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01417-4-prmt1-primary-antibodies-fcm-testing-3.pngAnti-PRMT1 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-PRMT1 antibody (A01417-4). Overlay histogram showing U937 cells stained with A01417-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT1 Antibody (A01417-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ndst4-picoband-trade-antibody-a11749-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11749-1-ndst4-primary-antibodies-wb-testing-1.jpgAnti-NDST4 Antibody Picoband® Western blot analysis of NDST4 using anti-NDST4 antibody (A11749-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDST4 antigen affinity purified polyclonal antibody (Catalog # A11749-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDST4 at approximately 101 kDa. The expected band size for NDST4 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11749-1-ndst4-primary-antibodies-fcm-testing-2.pngAnti-NDST4 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-NDST4 antibody (A11749-1). Overlay histogram showing 293T cells stained with A11749-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDST4 Antibody (A11749-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufa5-picoband-trade-antibody-a09003-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09003-1-ndufa5-primary-antibodies-wb-testing-1.jpgAnti-NDUFA5 Antibody Picoband® Western blot analysis of NDUFA5 using anti-NDUFA5 antibody (A09003-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA5 antigen affinity purified polyclonal antibody (Catalog # A09003-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA5 at approximately 16 kDa. The expected band size for NDUFA5 is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09003-1-ndufa5-primary-antibodies-if-testing-2.jpgAnti-NDUFA5 Antibody Picoband® IF analysis of NDUFA5 using anti-NDUFA5 antibody (A09003-1). NDUFA5 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDUFA5 Antibody (A09003-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09003-1-ndufa5-primary-antibodies-fcm-testing-3.pngAnti-NDUFA5 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-NDUFA5 antibody (A09003-1). Overlay histogram showing U937 cells stained with A09003-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA5 Antibody (A09003-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufa7-picoband-trade-antibody-a10817-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10817-1-ndufa7-primary-antibodies-ihc-testing-3.jpgAnti-NDUFA7 Antibody Picoband®IHC analysis of NDUFA7 using anti-NDUFA7 antibody (A10817-1). NDUFA7 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA7 Antibody (A10817-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10817-1-ndufa7-primary-antibodies-wb-testing-1.jpgAnti-NDUFA7 Antibody Picoband® Western blot analysis of NDUFA7 using anti-NDUFA7 antibody (A10817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA7 antigen affinity purified polyclonal antibody (Catalog # A10817-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA7 at approximately 15 kDa. The expected band size for NDUFA7 is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10817-1-ndufa7-primary-antibodies-ihc-testing-2_1.jpgAnti-NDUFA7 Antibody Picoband® IHC analysis of NDUFA7 using anti-NDUFA7 antibody (A10817-1). NDUFA7 was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA7 Antibody (A10817-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10817-1-ndufa7-primary-antibodies-fcm-testing-3.pngAnti-NDUFA7 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-NDUFA7 antibody (A10817-1). Overlay histogram showing SiHa cells stained with A10817-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA7 Antibody (A10817-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ovol2-picoband-trade-antibody-a07631-2-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07631-2-ovol2-primary-antibodies-wb-testing-1.jpgAnti-OVOL2 Antibody Picoband® Western blot analysis of OVOL2 using anti-OVOL2 antibody (A07631-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OVOL2 antigen affinity purified polyclonal antibody (Catalog # A07631-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OVOL2 at approximately 37 kDa. The expected band size for OVOL2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07631-2-ovol2-primary-antibodies-if-testing-2.jpgAnti-OVOL2 Antibody Picoband® IF analysis of OVOL2 using anti-OVOL2 antibody (A07631-2) and anti-Tubulin Alpha antibody (M03989-3). OVOL2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OVOL2 Antibody (A07631-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07631-2-ovol2-primary-antibodies-fcm-testing-3.pngAnti-OVOL2 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-OVOL2 antibody (A07631-2). Overlay histogram showing JK cells stained with A07631-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OVOL2 Antibody (A07631-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-omd-picoband-trade-antibody-a05351-1-boster.html2026-03-17T05:14:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05351-1-omd-primary-antibodies-wb-testing-1.jpgAnti-OMD Antibody Picoband® Western blot analysis of OMD using anti-OMD antibody (A05351-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: mouse bone tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMD antigen affinity purified polyclonal antibody (Catalog # A05351-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMD at approximately 60 kDa. The expected band size for OMD is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05351-1-omd-primary-antibodies-fcm-testing-2.pngAnti-OMD Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-OMD antibody (A05351-1). Overlay histogram showing U20S cells stained with A05351-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OMD Antibody (A05351-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pianp-picoband-trade-antibody-a14063-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14063-1-pianp-primary-antibodies-wb-testing-1.jpgAnti-PIANP Antibody Picoband® Western blot analysis of PIANP using anti-PIANP antibody (A14063-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIANP antigen affinity purified polyclonal antibody (Catalog # A14063-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIANP at approximately 45 kDa. The expected band size for PIANP is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14063-1-pianp-primary-antibodies-fcm-testing-2.pngAnti-PIANP Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PIANP antibody (A14063-1). Overlay histogram showing JK cells stained with A14063-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PIANP Antibody (A14063-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pigv-picoband-trade-antibody-a08698-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08698-1-pigv-primary-antibodies-wb-testing-1.jpgAnti-PIGV Antibody Picoband® Western blot analysis of PIGV using anti-PIGV antibody (A08698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGV antigen affinity purified polyclonal antibody (Catalog # A08698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGV at approximately 60 kDa. The expected band size for PIGV is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08698-1-pigv-primary-antibodies-fcm-testing-2.pngAnti-PIGV Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-PIGV antibody (A08698-1). Overlay histogram showing U937 cells stained with A08698-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIGV Antibody (A08698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pilra-picoband-trade-antibody-a07502-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07502-1-pilra-primary-antibodies-wb-testing-1.jpgAnti-PILRA Antibody Picoband® Western blot analysis of PILRA using anti-PILRA antibody (A07502-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PILRA antigen affinity purified polyclonal antibody (Catalog # A07502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PILRA at approximately 61 kDa. The expected band size for PILRA is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07502-1-pilra-primary-antibodies-fcm-testing-2.pngAnti-PILRA Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PILRA antibody (A07502-1). Overlay histogram showing HEL cells stained with A07502-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PILRA Antibody (A07502-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pld6-picoband-trade-antibody-a10904-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10904-1-pld6-primary-antibodies-wb-testing-1.jpgAnti-PLD6 Antibody Picoband® Western blot analysis of PLD6 using anti-PLD6 antibody (A10904-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD6 antigen affinity purified polyclonal antibody (Catalog # A10904-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD6 at approximately 28 kDa. The expected band size for PLD6 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10904-1-pld6-primary-antibodies-ihc-testing-2.jpgAnti-PLD6 Antibody Picoband® IHC analysis of PLD6 using anti-PLD6 antibody (A10904-1). PLD6 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLD6 Antibody (A10904-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10904-1-pld6-primary-antibodies-ihc-testing-3.jpgAnti-PLD6 Antibody Picoband® IHC analysis of PLD6 using anti-PLD6 antibody (A10904-1). PLD6 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLD6 Antibody (A10904-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10904-1-pld6-primary-antibodies-fcm-testing-4.pngAnti-PLD6 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PLD6 antibody (A10904-1). Overlay histogram showing MCF-7 cells stained with A10904-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLD6 Antibody (A10904-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10904-1-pld6-primary-antibodies-if-testing-5.jpgAnti-PLD6 Antibody Picoband® IF analysis of PLD6 using anti-PLD6 antibody (A10904-1). PLD6 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLD6 Antibody (A10904-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-plet1-picoband-trade-antibody-a14923-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14923-1-plet1-primary-antibodies-wb-testing-1.jpgAnti-PLET1 Antibody Picoband® Western blot analysis of PLET1 using anti-PLET1 antibody (A14923-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLET1 antigen affinity purified polyclonal antibody (Catalog # A14923-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLET1 at approximately 28 kDa. The expected band size for PLET1 is at 23,25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14923-1-plet1-primary-antibodies-fcm-testing-2.pngAnti-PLET1 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PLET1 antibody (A14923-1). Overlay histogram showing SiHa cells stained with A14923-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLET1 Antibody (A14923-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-plasmolipin-pllp-picoband-trade-antibody-a10793-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10793-pllp-primary-antibodies-wb-testing-1.jpgAnti-Plasmolipin/PLLP Antibody Picoband® Western blot analysis of Plasmolipin/PLLP using anti-Plasmolipin/PLLP antibody (A10793). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plasmolipin/PLLP antigen affinity purified polyclonal antibody (Catalog # A10793) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plasmolipin/PLLP at approximately 26 kDa. The expected band size for Plasmolipin/PLLP is at 18,20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10793-pllp-primary-antibodies-fcm-testing-2.pngAnti-Plasmolipin/PLLP Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Plasmolipin/PLLP antibody (A10793). Overlay histogram showing HepG2 cells stained with A10793 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Plasmolipin/PLLP Antibody (A10793, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-lypla3-pla2g15-picoband-trade-antibody-a08649-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08649-pla2g15-primary-antibodies-wb-testing-1.jpgAnti-LYPLA3/PLA2G15 Antibody Picoband® Western blot analysis of LYPLA3/PLA2G15 using anti-LYPLA3/PLA2G15 antibody (A08649). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA3/PLA2G15 antigen affinity purified polyclonal antibody (Catalog # A08649) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYPLA3/PLA2G15 at approximately 42-57 kDa. The expected band size for LYPLA3/PLA2G15 is at 65-70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08649-pla2g15-primary-antibodies-if-testing-2.jpgAnti-LYPLA3/PLA2G15 Antibody Picoband® IF analysis of LYPLA3/PLA2G15 using anti-LYPLA3/PLA2G15 antibody (A08649) and anti-Tubulin Alpha antibody (M03989-3). LYPLA3/PLA2G15 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LYPLA3/PLA2G15 Antibody (A08649) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-pnliprp2-picoband-trade-antibody-a11017-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11017-1-pnliprp2-primary-antibodies-wb-testing-1.jpgAnti-PNLIPRP2 Antibody Picoband® Western blot analysis of PNLIPRP2 using anti-PNLIPRP2 antibody (A11017-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNLIPRP2 antigen affinity purified polyclonal antibody (Catalog # A11017-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PNLIPRP2 at approximately 52 kDa. The expected band size for PNLIPRP2 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11017-1-pnliprp2-primary-antibodies-if-testing-2.jpgAnti-PNLIPRP2 Antibody Picoband® IF analysis of PNLIPRP2 using anti-PNLIPRP2 antibody (A11017-1). PNLIPRP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PNLIPRP2 Antibody (A11017-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11017-1-pnliprp2-primary-antibodies-fcm-testing-3.pngAnti-PNLIPRP2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PNLIPRP2 antibody (A11017-1). Overlay histogram showing Hela cells stained with A11017-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNLIPRP2 Antibody (A11017-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-polr3gl-picoband-trade-antibody-a17067-1-boster.html2026-04-03T05:00:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17067-1-polr3gl-primary-antibodies-wb-testing-1.jpgAnti-POLR3GL Antibody Picoband® Western blot analysis of POLR3GL using anti-POLR3GL antibody (A17067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR3GL antigen affinity purified polyclonal antibody (Catalog # A17067-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR3GL at approximately 24 kDa. The expected band size for POLR3GL is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17067-1-polr3gl-primary-antibodies-if-testing-2.jpgAnti-POLR3GL Antibody Picoband® IF analysis of POLR3GL using anti-POLR3GL antibody (A17067-1). POLR3GL was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POLR3GL Antibody (A17067-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-pomp-picoband-trade-antibody-a04837-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04837-1-pomp-primary-antibodies-wb-testing-1.jpgAnti-POMP Antibody Picoband® Western blot analysis of POMP using anti-POMP antibody (A04837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMP antigen affinity purified polyclonal antibody (Catalog # A04837-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMP at approximately 16 kDa. The expected band size for POMP is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04837-1-pomp-primary-antibodies-fcm-testing-2.pngAnti-POMP Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-POMP antibody (A04837-1). Overlay histogram showing A431 cells stained with A04837-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POMP Antibody (A04837-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppm1n-picoband-trade-antibody-a16917-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16917-1-ppm1n-primary-antibodies-wb-testing-1.jpgAnti-PPM1N Antibody Picoband® Western blot analysis of PPM1N using anti-PPM1N antibody (A16917-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1N antigen affinity purified polyclonal antibody (Catalog # A16917-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1N at approximately 45 kDa. The expected band size for PPM1N is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16917-1-ppm1n-primary-antibodies-if-testing-2.jpgAnti-PPM1N Antibody Picoband® IF analysis of PPM1N using anti-PPM1N antibody (A16917-1). PPM1N was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPM1N Antibody (A16917-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16917-1-ppm1n-primary-antibodies-fcm-testing-3.pngAnti-PPM1N Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PPM1N antibody (A16917-1). Overlay histogram showing MCF-7 cells stained with A16917-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1N Antibody (A16917-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp1r15b-picoband-trade-antibody-a10225-1-boster.html2026-03-17T05:14:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10225-1-ppp1r15b-primary-antibodies-wb-testing-1.jpgAnti-PPP1R15B Antibody Picoband® Western blot analysis of PPP1R15B using anti-PPP1R15B antibody (A10225-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R15B antigen affinity purified polyclonal antibody (Catalog # A10225-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R15B at approximately 110 kDa. The expected band size for PPP1R15B is at 79,100-110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10225-1-ppp1r15b-primary-antibodies-if-testing-2.jpgAnti-PPP1R15B Antibody Picoband® IF analysis of PPP1R15B using anti-PPP1R15B antibody (A10225-1). PPP1R15B was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP1R15B Antibody (A10225-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10225-1-ppp1r15b-primary-antibodies-fcm-testing-3.pngAnti-PPP1R15B Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PPP1R15B antibody (A10225-1). Overlay histogram showing MCF-7 cells stained with A10225-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R15B Antibody (A10225-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp1r36-picoband-trade-antibody-a17066-1-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17066-1-ppp1r36-primary-antibodies-wb-testing-1.jpgAnti-PPP1R36 Antibody Picoband® Western blot analysis of PPP1R36 using anti-PPP1R36 antibody (A17066-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R36 antigen affinity purified polyclonal antibody (Catalog # A17066-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R36 at approximately 54 kDa. The expected band size for PPP1R36 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17066-1-ppp1r36-primary-antibodies-if-testing-2.jpgAnti-PPP1R36 Antibody Picoband® IF analysis of PPP1R36 using anti-PPP1R36 antibody (A17066-1) and anti-Tubulin Alpha antibody (M03989-3). PPP1R36 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP1R36 Antibody (A17066-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17066-1-ppp1r36-primary-antibodies-fcm-testing-3.pngAnti-PPP1R36 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PPP1R36 antibody (A17066-1). Overlay histogram showing Hela cells stained with A17066-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R36 Antibody (A17066-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prdm8-picoband-trade-antibody-a10969-2-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10969-2-prdm8-primary-antibodies-wb-testing-1_1.jpgAnti-PRDM8 Antibody Picoband®Western blot analysis of PRDM8 using anti-PRDM8 antibody (A10969-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM8 antigen affinity purified polyclonal antibody (Catalog # A10969-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRDM8 at approximately 72 kDa. The expected band size for PRDM8 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10969-2-prdm8-primary-antibodies-if-testing-1.jpgAnti-PRDM8 Antibody Picoband®IF analysis of PRDM8 using anti-PRDM8 antibody (A10969-2). PRDM8 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRDM8 Antibody (A10969-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-prex2-picoband-trade-antibody-a06715-1-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06715-1-prex2-primary-antibodies-wb-testing-1.jpgAnti-PREX2 Antibody Picoband® Western blot analysis of PREX2 using anti-PREX2 antibody (A06715-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skeletal muscle lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX2 antigen affinity purified polyclonal antibody (Catalog # A06715-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX2 at approximately 183 kDa. The expected band size for PREX2 is at 183 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-prkce-picoband-trade-antibody-a01151-1-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01151-1-prcke-primary-antibodies-wb-testing-1.jpgAnti-PRKCE Antibody Picoband® Western blot analysis of PRKCE using anti-PRKCE antibody (A01151-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCE antigen affinity purified polyclonal antibody (Catalog # A01151-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCE at approximately 94 kDa. The expected band size for PRKCE is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01151-1-prcke-primary-antibodies-if-testing-2.jpgAnti-PRKCE Antibody Picoband® IF analysis of PRKCE4 using anti-PRKCE4 antibody (A01151-1) and anti-Tubulin Alpha antibody (M03989-3). PRKCE4 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRKCE4 Antibody (A01151-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-prkcsh-picoband-trade-antibody-a04992-2-boster.html2026-03-17T05:16:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04992-2-prkcsh-primary-antibodies-wb-testing-1_1.jpgAnti-PRKCSH Antibody Picoband® Western blot analysis of PRKCSH using anti-PRKCSH antibody (A04992-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCSH antigen affinity purified polyclonal antibody (Catalog # A04992-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCSH at approximately 90 kDa. The expected band size for PRKCSH is at 59,80-90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04992-2-prkcsh-primary-antibodies-if-testing-2.jpgAnti-PRKCSH Antibody Picoband® IF analysis of PRKCSH using anti-PRKCSH antibody (A04992-2). PRKCSH was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRKCSH Antibody (A04992-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04992-2-prkcsh-primary-antibodies-fcm-testing-3.pngAnti-PRKCSH Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PRKCSH antibody (A04992-2). Overlay histogram showing SiHa cells stained with A04992-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRKCSH Antibody (A04992-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp1r11-picoband-trade-antibody-a10474-2-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10474-2-ppp1r11-primary-antibodies-wb-testing-1.jpgAnti-PPP1R11 Antibody Picoband® Western blot analysis of PPP1R11 using anti-PPP1R11 antibody (A10474-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R11 antigen affinity purified polyclonal antibody (Catalog # A10474-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R11 at approximately 25 kDa. The expected band size for PPP1R11 is at 14,18,25-30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10474-2-ppp1r11-primary-antibodies-fcm-testing-2.pngAnti-PPP1R11 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PPP1R11 antibody (A10474-2). Overlay histogram showing MCF-7 cells stained with A10474-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R11 Antibody (A10474-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pigs-picoband-trade-antibody-a06942-1-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06942-1-pigs-primary-antibodies-wb-testing-1.jpgAnti-PIGS Antibody Picoband® Western blot analysis of PIGS using anti-PIGS antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue(HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue(HCCP) lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGS antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGS at approximately 65 kDa. The expected band size for PIGS is at 62,65-70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06942-1-pigs-primary-antibodies-if-testing-2.jpgAnti-PIGS Antibody Picoband® IF analysis of PIGS using anti-PIGS antibody (A06942-1). PIGS was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PIGS Antibody (A06942-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06942-1-pigs-primary-antibodies-fcm-testing-3.pngAnti-PIGS Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-PIGS antibody (A06942-1). Overlay histogram showing RT4 cells stained with A06942-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIGS Antibody (A06942-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-apoe-picoband-trade-antibody-a00015-5-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00015-5-apoe-primary-antibodies-wb-testing-1.jpgAnti-APOE Antibody Picoband® Western blot analysis of APOE using anti-APOE antibody (A00015-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOE antigen affinity purified polyclonal antibody (Catalog # A00015-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOE at approximately 36 kDa. The expected band size for APOE is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00015-5-apoe-primary-antibodies-ihc-testing-1.jpgAnti-APOE Antibody Picoband®IHC analysis of APOE using anti-APOE antibody (A00015-5). APOE was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APOE Antibody (A00015-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00015-5-apoe-primary-antibodies-if-testing-2.jpgAnti-APOE Antibody Picoband® IF analysis of APOE using anti-APOE antibody (A00015-5). APOE was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-APOE Antibody (A00015-5) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00015-5-apoe-primary-antibodies-fcm-testing-3.pngAnti-APOE Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-APOE antibody (A00015-5). Overlay histogram showing CACO-2 cells stained with A00015-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-APOE Antibody (A00015-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-integrin-beta-1-itgb1-picoband-trade-antibody-a00772-3-boster.html2026-03-17T05:14:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00772-3-itgb1-primary-antibodies-wb-testing-1.jpgAnti-Integrin Beta 1/ITGB1 Antibody Picoband® Western blot analysis of Integrin Beta 1/ITGB1 using anti-Integrin Beta 1/ITGB1 antibody (A00772-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Beta 1/ITGB1 antigen affinity purified polyclonal antibody (Catalog # A00772-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Beta 1/ITGB1 at approximately 88,115-140 kDa. The expected band size for Integrin Beta 1/ITGB1 is at 88 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-narg1l-naa16-picoband-trade-antibody-a13221-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13221-1-naa16-primary-antibodies-wb-testing-1.jpgAnti-NARG1L/NAA16 Antibody Picoband® Western blot analysis of NARG1L/NAA16 using anti-NARG1L/NAA16 antibody (A13221-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARG1L/NAA16 antigen affinity purified polyclonal antibody (Catalog # A13221-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARG1L/NAA16 at approximately 130 kDa. The expected band size for NARG1L/NAA16 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13221-1-naa16-primary-antibodies-fcm-testing-2.pngAnti-NARG1L/NAA16 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-NARG1L/NAA16 antibody (A13221-1). Overlay histogram showing HepG2 cells stained with A13221-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NARG1L/NAA16 Antibody (A13221-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-naaa-picoband-trade-antibody-a06195-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06195-1-naaa-primary-antibodies-wb-testing-1.jpgAnti-NAAA Antibody Picoband® Western blot analysis of NAAA using anti-NAAA antibody (A06195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAAA antigen affinity purified polyclonal antibody (Catalog # A06195-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAAA at approximately 38 kDa. The expected band size for NAAA is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06195-1-naaa-primary-antibodies-fcm-testing-2.pngAnti-NAAA Antibody Picoband® Flow Cytometry analysis of JK cells using anti-NAAA antibody (A06195-1). Overlay histogram showing JK cells stained with A06195-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAAA Antibody (A06195-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nab1-picoband-trade-antibody-a06669-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06669-1-nab1-primary-antibodies-wb-testing-1.jpgAnti-NAB1 Antibody Picoband® Western blot analysis of NAB1 using anti-NAB1 antibody (A06669-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAB1 antigen affinity purified polyclonal antibody (Catalog # A06669-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAB1 at approximately 56 kDa. The expected band size for NAB1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06669-1-nab1-primary-antibodies-if-testing-2.jpgAnti-NAB1 Antibody Picoband® IF analysis of NAB1 using anti-NAB1 antibody (A06669-1) and anti-Beta Tubulin antibody (M01857-3). NAB1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NAB1 Antibody (A06669-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-c5orf33-nadk2-picoband-trade-antibody-a09619-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09619-1-nadk2-primary-antibodies-wb-testing-1.jpgAnti-C5orf33/NADK2 Antibody Picoband® Western blot analysis of C5orf33/NADK2 using anti-C5orf33/NADK2 antibody (A09619-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5orf33/NADK2 antigen affinity purified polyclonal antibody (Catalog # A09619-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5orf33/NADK2 at approximately 53 kDa. The expected band size for C5orf33/NADK2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09619-1-nadk2-primary-antibodies-if-testing-2.jpgAnti-C5orf33/NADK2 Antibody Picoband® IF analysis of C5orf33/NADK2 using anti-C5orf33/NADK2 antibody (A09619-1). C5orf33/NADK2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C5orf33/NADK2 Antibody (A09619-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09619-1-nadk2-primary-antibodies-fcm-testing-3.pngAnti-C5orf33/NADK2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-C5orf33/NADK2 antibody (A09619-1). Overlay histogram showing HepG2 cells stained with A09619-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C5orf33/NADK2 Antibody (A09619-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-grim19-ndufa13-picoband-trade-antibody-a05981-2-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05981-2-ndufa13-primary-antibodies-wb-testing-1.jpgAnti-GRIM19/NDUFA13 Antibody Picoband® Western blot analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (A05981-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIM19/NDUFA13 antigen affinity purified polyclonal antibody (Catalog # A05981-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIM19/NDUFA13 at approximately 16,17 kDa. The expected band size for GRIM19/NDUFA13 is at 16-18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05981-2-ndufa13-primary-antibodies-ihc-testing-2.jpgAnti-GRIM19/NDUFA13 Antibody Picoband® IHC analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (A05981-2). GRIM19/NDUFA13 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRIM19/NDUFA13 Antibody (A05981-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05981-2-ndufa13-primary-antibodies-ihc-testing-3.jpgAnti-GRIM19/NDUFA13 Antibody Picoband® IHC analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (A05981-2). GRIM19/NDUFA13 was detected in a paraffin-embedded section of human squamous cell carcinoma of skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRIM19/NDUFA13 Antibody (A05981-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05981-2-ndufa13-primary-antibodies-ihc-testing-4.jpgAnti-GRIM19/NDUFA13 Antibody Picoband® IHC analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (A05981-2). GRIM19/NDUFA13 was detected in a paraffin-embedded section of human urothelial carcinoma with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRIM19/NDUFA13 Antibody (A05981-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05981-2-ndufa13-primary-antibodies-if-testing-5.jpgAnti-GRIM19/NDUFA13 Antibody Picoband® IF analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (A05981-2). GRIM19/NDUFA13 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GRIM19/NDUFA13 Antibody (A05981-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05981-2-ndufa13-primary-antibodies-if-testing-6.jpgAnti-GRIM19/NDUFA13 Antibody Picoband® IF analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (A05981-2). GRIM19/NDUFA13 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GRIM19/NDUFA13 Antibody (A05981-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05981-2-ndufa13-primary-antibodies-fcm-testing-7.pngAnti-GRIM19/NDUFA13 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-GRIM19/NDUFA13 antibody (A05981-2). Overlay histogram showing U937 cells stained with A05981-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIM19/NDUFA13 Antibody (A05981-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufa2-picoband-trade-antibody-a10396-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10396-1-ndufa2-primary-antibodies-wb-testing-1.jpgAnti-NDUFA2 Antibody Picoband® Western blot analysis of NDUFA2 using anti-NDUFA2 antibody (A10396-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA2 antigen affinity purified polyclonal antibody (Catalog # A10396-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA2 at approximately 14 kDa. The expected band size for NDUFA2 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ndufa3-picoband-trade-antibody-a11694-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11694-1-ndufa3-primary-antibodies-wb-testing-1.jpgAnti-NDUFA3 Antibody Picoband® Western blot analysis of NDUFA3 using anti-NDUFA3 antibody (A11694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA3 antigen affinity purified polyclonal antibody (Catalog # A11694-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA3 at approximately 9 kDa. The expected band size for NDUFA3 is at 9 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ndufaf4-picoband-trade-antibody-a11650-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-wb-testing-1.jpgAnti-NDUFAF4 Antibody Picoband® Western blot analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Ramos whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAF4 antigen affinity purified polyclonal antibody (Catalog # A11650-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAF4 at approximately 21 kDa. The expected band size for NDUFAF4 is at 20,21,25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-ihc-testing-2.jpgAnti-NDUFAF4 Antibody Picoband® IHC analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). NDUFAF4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFAF4 Antibody (A11650-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-ihc-testing-3.jpgAnti-NDUFAF4 Antibody Picoband® IHC analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). NDUFAF4 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFAF4 Antibody (A11650-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-ihc-testing-4.jpgAnti-NDUFAF4 Antibody Picoband® IHC analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). NDUFAF4 was detected in a paraffin-embedded section of human ovarian tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFAF4 Antibody (A11650-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-ihc-testing-5.jpgAnti-NDUFAF4 Antibody Picoband® IHC analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). NDUFAF4 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFAF4 Antibody (A11650-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-if-testing-6.jpgAnti-NDUFAF4 Antibody Picoband® IF analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). NDUFAF4 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDUFAF4 Antibody (A11650-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-if-testing-7.jpgAnti-NDUFAF4 Antibody Picoband® IF analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). NDUFAF4 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFAF4 Antibody (A11650-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11650-1-ndufaf4-primary-antibodies-fcm-testing-8.pngAnti-NDUFAF4 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-NDUFAF4 antibody (A11650-1). Overlay histogram showing U937 cells stained with A11650-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFAF4 Antibody (A11650-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufb7-picoband-trade-antibody-a10231-1-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10231-1-ndufb7-primary-antibodies-wb-testing-1_1.jpgAnti-NDUFB7 Antibody Picoband®Western blot analysis of NDUFB7 using anti-NDUFB7 antibody (A10231-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB7 antigen affinity purified polyclonal antibody (A10231-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDUFB7 at approximately 19 kDa. The expected band size for NDUFB7 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10231-1-ndufb7-primary-antibodies-ihc-testing-1.jpgAnti-NDUFB7 Antibody Picoband®IHC analysis of NDUFB7 using anti-NDUFB7 antibody (A10231-1). NDUFB7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFB7 Antibody (A10231-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10231-1-ndufb7-primary-antibodies-ihc-testing-2_1.jpgAnti-NDUFB7 Antibody Picoband®IHC analysis of NDUFB7 using anti-NDUFB7 antibody (A10231-1). NDUFB7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFB7 Antibody (A10231-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10231-1-ndufb7-primary-antibodies-if-testing-1.jpgAnti-NDUFB7 Antibody Picoband®IF analysis of NDUFB7 using anti-NDUFB7 antibody (A10231-1). NDUFB7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-NDUFB7 Antibody (A10231-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10231-1-ndufb7-primary-antibodies-fcm-testing-1.jpgAnti-NDUFB7 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-NDUFB7 antibody (A10231-1). Overlay histogram showing PC-3 cells stained with A10231-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB7 Antibody (A10231-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-oprm1-picoband-trade-antibody-a00737-2-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00737-2-oprm1-primary-antibodies-wb-testing-1.jpgAnti-OPRM1 Antibody Picoband® Western blot analysis of OPRM1 using anti-OPRM1 antibody (A00737-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPRM1 antigen affinity purified polyclonal antibody (Catalog # A00737-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPRM1 at approximately 70 kDa. The expected band size for OPRM1 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-osbpl10-picoband-trade-antibody-a07484-2-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07484-2-osbpl10-primary-antibodies-wb-testing-1.jpgAnti-OSBPL10 Antibody Picoband® Western blot analysis of OSBPL10 using anti-OSBPL10 antibody (A07484-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL10 antigen affinity purified polyclonal antibody (Catalog # A07484-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL10 at approximately 84 kDa. The expected band size for OSBPL10 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07484-2-osbpl10-primary-antibodies-if-testing-2.jpgAnti-OSBPL10 Antibody Picoband® IF analysis of OSBPL10 using anti-OSBPL10 antibody (A07484-2). OSBPL10 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OSBPL10 Antibody (A07484-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07484-2-osbpl10-primary-antibodies-fcm-testing-3.pngAnti-OSBPL10 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-OSBPL10 antibody (A07484-2). Overlay histogram showing HepG2 cells stained with A07484-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OSBPL10 Antibody (A07484-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pdcd4-picoband-trade-antibody-a01105-4-boster.html2026-03-17T05:14:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01105-4-pdcd4-primary-antibodies-wb-testing-1.jpgAnti-PDCD4 Antibody Picoband® Western blot analysis of PDCD4 using anti-PDCD4 antibody (A01105-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD4 antigen affinity purified polyclonal antibody (Catalog # A01105-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDCD4 at approximately 64 kDa. The expected band size for PDCD4 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pram1-picoband-trade-antibody-a10037-2-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10037-2-pram1-primary-antibodies-wb-testing-1.jpgAnti-PRAM1 Antibody Picoband® Western blot analysis of PRAM1 using anti-PRAM1 antibody (A10037-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAM1 antigen affinity purified polyclonal antibody (Catalog # A10037-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAM1 at approximately 90 kDa. The expected band size for PRAM1 is at 70,74,90-100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10037-2-pram1-primary-antibodies-fcm-testing-2.pngAnti-PRAM1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PRAM1 antibody (A10037-2). Overlay histogram showing JK cells stained with A10037-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRAM1 Antibody (A10037-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prpf18-picoband-trade-antibody-a13674-2-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-wb-testing-1.jpgAnti-PRPF18 Antibody Picoband® Western blot analysis of PRPF18 using anti-PRPF18 antibody (A13674-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF18 antigen affinity purified polyclonal antibody (Catalog # A13674-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF18 at approximately 42 kDa. The expected band size for PRPF18 is at 40,42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-ihc-testing-2.jpgAnti-PRPF18 Antibody Picoband® IHC analysis of PRPF18 using anti-PRPF18 antibody (A13674-2). PRPF18 was detected in a paraffin-embedded section of human ovary serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF18 Antibody (A13674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-ihc-testing-3.jpgAnti-PRPF18 Antibody Picoband® IHC analysis of PRPF18 using anti-PRPF18 antibody (A13674-2). PRPF18 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF18 Antibody (A13674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-ihc-testing-4.jpgAnti-PRPF18 Antibody Picoband® IHC analysis of PRPF18 using anti-PRPF18 antibody (A13674-2). PRPF18 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF18 Antibody (A13674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-ihc-testing-5.jpgAnti-PRPF18 Antibody Picoband® IHC analysis of PRPF18 using anti-PRPF18 antibody (A13674-2). PRPF18 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF18 Antibody (A13674-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-if-testing-6.jpgAnti-PRPF18 Antibody Picoband® IF analysis of PRPF18 using anti-PRPF18 antibody (A13674-2). PRPF18 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRPF18 Antibody (A13674-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-fcm-testing-7.pngAnti-PRPF18 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PRPF18 antibody (A13674-2). Overlay histogram showing JK cells stained with A13674-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF18 Antibody (A13674-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13674-2-prpf18-primary-antibodies-fcm-testing-8.pngAnti-PRPF18 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PRPF18 antibody (A13674-2). Overlay histogram showing THP-1 cells stained with A13674-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF18 Antibody (A13674-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prpf19-picoband-trade-antibody-a02434-3-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02434-3-prpf19-primary-antibodies-wb-testing-1.jpgAnti-PRPF19 Antibody Picoband® Western blot analysis of PRPF19 using anti-PRPF19 antibody (A02434-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF19 antigen affinity purified polyclonal antibody (Catalog # A02434-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF19 at approximately 54 kDa. The expected band size for PRPF19 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02434-3-prpf19-primary-antibodies-if-testing-2.jpgAnti-PRPF19 Antibody Picoband® IF analysis of PRPF19 using anti-PRPF19 antibody (A02434-3). PRPF19 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRPF19 Antibody (A02434-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02434-3-prpf19-primary-antibodies-fcm-testing-3.pngAnti-PRPF19 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PRPF19 antibody (A02434-3). Overlay histogram showing JK cells stained with A02434-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF19 Antibody (A02434-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prpf31-picoband-trade-antibody-a03137-1-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03137-1-prpf31-primary-antibodies-wb-testing-1.jpgAnti-PRPF31 Antibody Picoband® Western blot analysis of PRPF31 using anti-PRPF31 antibody (A03137-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF31 antigen affinity purified polyclonal antibody (Catalog # A03137-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF31 at approximately 60 kDa. The expected band size for PRPF31 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03137-1-prpf31-primary-antibodies-if-testing-2.jpgAnti-PRPF31 Antibody Picoband® IF analysis of PRPF31 using anti-PRPF31 antibody (A03137-1). PRPF31 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRPF31 Antibody (A03137-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03137-1-prpf31-primary-antibodies-fcm-testing-3.pngAnti-PRPF31 Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-PRPF31 antibody (A03137-1). Overlay histogram showing U937 cells stained with A03137-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF31 Antibody (A03137-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prpf39-picoband-trade-antibody-a15727-2-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15727-2-prpf39-primary-antibodies-wb-testing-1.jpgAnti-PRPF39 Antibody Picoband® Western blot analysis of PRPF39 using anti-PRPF39 antibody (A15727-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF39 antigen affinity purified polyclonal antibody (Catalog # A15727-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF39 at approximately 90 kDa. The expected band size for PRPF39 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15727-2-prpf39-primary-antibodies-if-testing-2.jpgAnti-PRPF39 Antibody Picoband® IF analysis of PRPF39 using anti-PRPF39 antibody (A15727-2). PRPF39 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRPF39 Antibody (A15727-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15727-2-prpf39-primary-antibodies-fcm-testing-3.pngAnti-PRPF39 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-PRPF39 antibody (A15727-2). Overlay histogram showing Raji cells stained with A15727-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF39 Antibody (A15727-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prpf6-picoband-trade-antibody-a06724-2-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06724-2-prpf6-primary-antibodies-wb-testing-1.jpgAnti-PRPF6 Antibody Picoband® Western blot analysis of PRPF6 using anti-PRPF6 antibody (A06724-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF6 antigen affinity purified polyclonal antibody (Catalog # A06724-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF6 at approximately 107 kDa. The expected band size for PRPF6 is at 107 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06724-2-prpf6-primary-antibodies-if-testing-2.jpgAnti-PRPF6 Antibody Picoband® IF analysis of PRPF6 using anti-PRPF6 antibody (A06724-2). PRPF6 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRPF6 Antibody (A06724-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06724-2-prpf6-primary-antibodies-fcm-testing-3.pngAnti-PRPF6 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PRPF6 antibody (A06724-2). Overlay histogram showing Hela cells stained with A06724-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF6 Antibody (A06724-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prpf8-picoband-trade-antibody-a02878-2-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02878-2-prpf8-primary-antibodies-wb-testing-1.jpgAnti-PRPF8 Antibody Picoband® Western blot analysis of PRPF8 using anti-PRPF8 antibody (A02878-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF8 antigen affinity purified polyclonal antibody (Catalog # A02878-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF8 at approximately 250 kDa. The expected band size for PRPF8 is at 274 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-prrg1-picoband-trade-antibody-a14451-1-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14451-1-prrg1-primary-antibodies-wb-testing-1.jpgAnti-PRRG1 Antibody Picoband® Western blot analysis of PRRG1 using anti-PRRG1 antibody (A14451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRRG1 antigen affinity purified polyclonal antibody (Catalog # A14451-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRRG1 at approximately 30 kDa. The expected band size for PRRG1 is at 24,25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14451-1-prrg1-primary-antibodies-fcm-testing-2.pngAnti-PRRG1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PRRG1 antibody (A14451-1). Overlay histogram showing HEL cells stained with A14451-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRRG1 Antibody (A14451-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prss22-picoband-trade-antibody-a13704-1-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13704-1-prss22-primary-antibodies-wb-testing-1_1.jpgAnti-PRSS22 Antibody Picoband® Western blot analysis of PRSS22 using anti-PRSS22 antibody (A13704-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS22 antigen affinity purified polyclonal antibody (Catalog # A13704-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS22 at approximately 42 kDa. The expected band size for PRSS22 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13704-1-prss22-primary-antibodiesfcm-testing-2.jpgAnti-PRSS22 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-PRSS22 antibody (A13704-1). Overlay histogram showing RT4 cells stained with A13704-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRSS22 Antibody (A13704-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prss27-picoband-trade-antibody-a14210-1-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14210-1-prss27-primary-antibodies-wb-testing-1.jpgAnti-PRSS27 Antibody Picoband® Western blot analysis of PRSS27 using anti-PRSS27 antibody (A14210-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse pancreas tissue lysates, Lane 3: mouse MFC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS27 antigen affinity purified polyclonal antibody (Catalog # A14210-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS27 at approximately 32 kDa. The expected band size for PRSS27 is at 28,32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14210-1-prss27-primary-antibodies-fcm-testing-2.pngAnti-PRSS27 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PRSS27 antibody (A14210-1). Overlay histogram showing JK cells stained with A14210-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRSS27 Antibody (A14210-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prss35-picoband-trade-antibody-a14920-1-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14920-1-prss35-primary-antibodies-wb-testing-1.jpgAnti-PRSS35 Antibody Picoband® Western blot analysis of PRSS35 using anti-PRSS35 antibody (A14920-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS35 antigen affinity purified polyclonal antibody (Catalog # A14920-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS35 at approximately 53 kDa. The expected band size for PRSS35 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-prss44-picoband-trade-antibody-a18279-1-boster.html2026-03-17T05:14:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18279-1-prss44-primary-antibodies-wb-testing-1.jpgAnti-PRSS44 Antibody Picoband® Western blot analysis of PRSS44 using anti-PRSS44 antibody (A18279-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS44 antigen affinity purified polyclonal antibody (Catalog # A18279-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS44 at approximately 45 kDa. The expected band size for PRSS44 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18279-1-prss44-primary-antibodies-if-testing-2.jpgAnti-PRSS44 Antibody Picoband® IF analysis of PRSS44 using anti-PRSS44 antibody (A18279-1). PRSS44 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRSS44 Antibody (A18279-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-prss8-picoband-trade-antibody-a05561-2-boster.html2026-03-17T05:14:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05561-2-prss8-primary-antibodies-wb-testing-1.jpgAnti-PRSS8 Antibody Picoband® Western blot analysis of PRSS8 using anti-PRSS8 antibody (A05561-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS8 antigen affinity purified polyclonal antibody (Catalog # A05561-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS8 at approximately 38 kDa. The expected band size for PRSS8 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05561-2-prss8-primary-antibodies-fcm-testing-2.pngAnti-PRSS8 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PRSS8 antibody (A05561-2). Overlay histogram showing MCF-7 cells stained with A05561-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRSS8 Antibody (A05561-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prtfdc1-picoband-trade-antibody-a11841-1-boster.html2026-03-17T05:14:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11841-1-prtfdc1-primary-antibodies-wb-testing-1_1.jpgAnti-PRTFDC1 Antibody Picoband®Western blot analysis of PRTFDC1 using anti-PRTFDC1 antibody (A11841-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRTFDC1 antigen affinity purified polyclonal antibody (A11841-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRTFDC1 at approximately 26 kDa. The expected band size for PRTFDC1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11841-1-prtfdc1-primary-antibodies-if-testing-1.jpgAnti-PRTFDC1 Antibody Picoband®Figure 6. IF analysis of PRTFDC1 using anti-PRTFDC1 antibody (A11841-1) and anti-Tubulin Alpha antibody (M03989-3). PRTFDC1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRTFDC1 Antibody (A11841-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11841-1-prtfdc1-primary-antibodies-ip-testing-1_1.jpgAnti-PRTFDC1 Antibody Picoband®Immunoprecipitating (IP) PRTFDC1 in A549 whole cell lysate. Western blot analysis of PRTFDC1 using anti-PRTFDC1 antibody (A11841-1); Lane 1: A549 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PRTFDC1 antibody in A549 whole cell lysate; Lane 3: anti-PRTFDC1 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRTFDC1 antigen affinity purified polyclonal antibody (A11841-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PRTFDC1 at approximately 26 kDa. The expected band size for PRTFDC1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11841-1-prtfdc1-primary-antibodies-fcm-testing-1.jpgAnti-PRTFDC1 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-PRTFDC1 antibody (A11841-1). Overlay histogram showing CACO-2 cells stained with A11841-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRTFDC1 Antibody (A11841-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-psap-picoband-trade-antibody-a00937-1-boster.html2026-03-17T05:14:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00937-1-psap-primary-antibodies-wb-testing-1.jpgAnti-PSAP Antibody Picoband® Western blot analysis of PSAP using anti-PSAP antibody (A00937-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAP antigen affinity purified polyclonal antibody (Catalog # A00937-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSAP at approximately 70 kDa. The expected band size for PSAP is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00937-1-psap-primary-antibodies-ihc-testing-2.jpgAnti-PSAP Antibody Picoband® IHC analysis of PSAP using anti-PSAP antibody (A00937-1). PSAP was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSAP Antibody (A00937-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00937-1-psap-primary-antibodies-ihc-testing-3.jpgAnti-PSAP Antibody Picoband® IHC analysis of PSAP using anti-PSAP antibody (A00937-1). PSAP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSAP Antibody (A00937-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00937-1-psap-primary-antibodies-if-testing-4.jpgAnti-PSAP Antibody Picoband® IF analysis of PSAP using anti-PSAP antibody (A00937-1). PSAP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSAP Antibody (A00937-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00937-1-psap-primary-antibodies-if-testing-5.jpgAnti-PSAP Antibody Picoband® IF analysis of PSAP using anti-PSAP antibody (A00937-1). PSAP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSAP Antibody (A00937-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00937-1-psap-primary-antibodies-fcm-testing-6.pngAnti-PSAP Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PSAP antibody (A00937-1). Overlay histogram showing MCF-7 cells stained with A00937-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCF-7 Antibody (A00937-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psat1-picoband-trade-antibody-a06277-boster.html2026-03-17T05:14:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06277-psat1-primary-antibodies-wb-testing-1.jpgAnti-PSAT1 Antibody Picoband® Western blot analysis of PSAT1 using anti-PSAT1 antibody (A06277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAT1 antigen affinity purified polyclonal antibody (Catalog # A06277) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSAT1 at approximately 40 kDa. The expected band size for PSAT1 is at 37-45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06277-psat1-primary-antibodies-if-testing-2.jpgAnti-PSAT1 Antibody Picoband® IF analysis of PSAT1 using anti-PSAT1 antibody (A06277). PSAT1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSAT1 Antibody (A06277) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06277-psat1-primary-antibodies-fcm-testing-3.pngAnti-PSAT1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PSAT1 antibody (A06277). Overlay histogram showing JK cells stained with A06277 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSAT1 Antibody (A06277, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psd2-picoband-trade-antibody-a12531-1-boster.html2026-03-17T05:14:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12531-1-psd2-primary-antibodies-wb-testing-1.jpgAnti-PSD2 Antibody Picoband® Western blot analysis of PSD2 using anti-PSD2 antibody (A12531-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD2 antigen affinity purified polyclonal antibody (Catalog # A12531-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD2 at approximately 95 kDa. The expected band size for PSD2 is at 85-95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12531-1-psd2-primary-antibodies-fcm-testing-2.pngAnti-PSD2 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-PSD2 antibody (A12531-1). Overlay histogram showing HEL cells stained with A12531-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD2 Antibody (A12531-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psd3-picoband-trade-antibody-a10329-1-boster.html2026-03-17T05:14:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10329-1-psd3-primary-antibodies-wb-testing-1.jpgAnti-PSD3 Antibody Picoband® Western blot analysis of PSD3 using anti-PSD3 antibody (A10329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD3 antigen affinity purified polyclonal antibody (Catalog # A10329-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD3 at approximately 130 kDa. The expected band size for PSD3 is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10329-1-psd3-primary-antibodies-fcm-testing-2.pngAnti-PSD3 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-PSD3 antibody (A10329-1). Overlay histogram showing RT4 cells stained with A10329-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD3 Antibody (A10329-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psma1-picoband-trade-antibody-a07591-1-boster.html2026-03-17T05:14:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07591-1-psma1-primary-antibodies-wb-testing-1.jpgAnti-PSMA1 Antibody Picoband® Western blot analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA1 antigen affinity purified polyclonal antibody (Catalog # A07591-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA1 at approximately 33 kDa. The expected band size for PSMA1 is at 29-33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07591-1-psma1-primary-antibodies-ihc-testing-2.jpgAnti-PSMA1 Antibody Picoband® IHC analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). PSMA1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (A07591-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07591-1-psma1-primary-antibodies-ihc-testing-3.jpgAnti-PSMA1 Antibody Picoband® IHC analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). PSMA1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (A07591-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07591-1-psma1-primary-antibodies-ihc-testing-4.jpgAnti-PSMA1 Antibody Picoband® IHC analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). PSMA1 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (A07591-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07591-1-psma1-primary-antibodies-ihc-testing-5.jpgAnti-PSMA1 Antibody Picoband® IHC analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). PSMA1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (A07591-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07591-1-psma1-primary-antibodies-ihc-testing-6.jpgAnti-PSMA1 Antibody Picoband® IHC analysis of PSMA1 using anti-PSMA1 antibody (A07591-1). PSMA1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (A07591-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07591-1-psma1-primary-antibodies-fcm-testing-7.pngAnti-PSMA1 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-PSMA1 antibody (A07591-1). Overlay histogram showing Raji cells stained with A07591-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMA1 Antibody (A07591-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psmb2-picoband-trade-antibody-a07697-1-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07697-1-psmb2-primary-antibodies-wb-testing-1_1.jpgAnti-PSMB2 Antibody Picoband® Western blot analysis of PSMB2 using anti-PSMB2 antibody (A07697-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB2 antigen affinity purified polyclonal antibody (Catalog # A07697-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB2 at approximately 23 kDa. The expected band size for PSMB2 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07697-1-psmb2-primary-antibodies-ihc-testing-1.jpgAnti-PSMB2 Antibody Picoband®IHC analysis of PSMB2 using anti-PSMB2 antibody (A07697-1). PSMB2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB2 Antibody (A07697-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07697-1-psmb2-primary-antibodies-if-testing-2_1.jpgAnti-PSMB2 Antibody Picoband® IF analysis of PSMB2 using anti-PSMB2 antibody (A07697-1). PSMB2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMB2 Antibody (A07697-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07697-1-psmb2-primary-antibodies-if-testing-1.jpgAnti-PSMB2 Antibody Picoband®IF analysis of PSMB2 using anti-PSMB2 antibody (A07697-1). PSMB2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMB2 Antibody (A07697-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-psmb9-picoband-trade-antibody-a02867-2-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02867-2-psmb9-primary-antibodies-wb-testing-1.jpgAnti-PSMB9 Antibody Picoband® Western blot analysis of PSMB9 using anti-PSMB9 antibody (A02867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB9 antigen affinity purified polyclonal antibody (Catalog # A02867-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB9 at approximately 21 kDa. The expected band size for PSMB9 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02867-2-psmb9-primary-antibodies-ihc-testing-2.jpgAnti-PSMB9 Antibody Picoband® IHC analysis of PSMB9 using anti-PSMB9 antibody (A02867-2). PSMB9 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB9 Antibody (A02867-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02867-2-psmb9-primary-antibodies-ihc-testing-3.jpgAnti-PSMB9 Antibody Picoband® IHC analysis of PSMB9 using anti-PSMB9 antibody (A02867-2). PSMB9 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB9 Antibody (A02867-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02867-2-psmb9-primary-antibodies-if-testing-4.jpgAnti-PSMB9 Antibody Picoband® IF analysis of PSMB9 using anti-PSMB9 antibody (A02867-2). PSMB9 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMB9 Antibody (A02867-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02867-2-psmb9-primary-antibodies-fcm-testing-5.pngAnti-PSMB9 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PSMB9 antibody (A02867-2). Overlay histogram showing JK cells stained with A02867-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB9 Antibody (A02867-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psmc2-picoband-trade-antibody-a09306-1-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09306-1-psmc2-primary-antibodies-wb-testing-1.jpgAnti-PSMC2 Antibody Picoband® Western blot analysis of PSMC2 using anti-PSMC2 antibody (A09306-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC2 antigen affinity purified polyclonal antibody (Catalog # A09306-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC2 at approximately 49 kDa. The expected band size for PSMC2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09306-1-psmc2-primary-antibodies-if-testing-2.jpgAnti-PSMC2 Antibody Picoband® IF analysis of PSMC2 using anti-PSMC2 antibody (A09306-1). PSMC2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMC2 Antibody (A09306-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-psmc4-picoband-trade-antibody-a06099-1-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-wb-testing-1.jpgAnti-PSMC4 Antibody Picoband® Western blot analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody (Catalog # A06099-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC4 at approximately 50 kDa. The expected band size for PSMC4 is at 43-50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-ihc-testing-2.jpgAnti-PSMC4 Antibody Picoband® IHC analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). PSMC4 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC4 Antibody (A06099-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-ihc-testing-3.jpgAnti-PSMC4 Antibody Picoband® IHC analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). PSMC4 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC4 Antibody (A06099-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-ihc-testing-4.jpgAnti-PSMC4 Antibody Picoband® IHC analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). PSMC4 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC4 Antibody (A06099-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-ihc-testing-5.jpgAnti-PSMC4 Antibody Picoband® IHC analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). PSMC4 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC4 Antibody (A06099-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-ihc-testing-6.jpgAnti-PSMC4 Antibody Picoband® IHC analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). PSMC4 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC4 Antibody (A06099-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-if-testing-7.jpgAnti-PSMC4 Antibody Picoband® IF analysis of PSMC4 using anti-PSMC4 antibody (A06099-1) and anti-Beta Tubulin antibody (M01857-3). PSMC4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMC4 Antibody (A06099-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-ip-testing-1.jpgAnti-PSMC4 Antibody Picoband®Immunoprecipitating (IP) PSMC4 in SH-SY5Y whole cell lysate. Western blot analysis of PSMC4 using anti-PSMC4 antibody (A06099-1); Lane 1: SH-SY5Y whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PSMC4 antibody in SH-SY5Y whole cell lysate; Lane 3: anti-PSMC4 antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody (A06099-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PSMC4 at approximately 47 kDa. The expected band size for PSMC4 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06099-1-psmc4-primary-antibodies-fcm-testing-8.pngAnti-PSMC4 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-PSMC4 antibody (A06099-1). Overlay histogram showing Raji cells stained with A06099-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMC4 Antibody (A06099-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psmc5-picoband-trade-antibody-a05480-1-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05480-1-psmc5-primary-antibodies-wb-testing-1.jpgAnti-PSMC5 Antibody Picoband® Western blot analysis of PSMC5 using anti-PSMC5 antibody (A05480-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC5 antigen affinity purified polyclonal antibody (Catalog # A05480-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC5 at approximately 45-49 kDa. The expected band size for PSMC5 is at 45-49 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-poh1-psmd14-picoband-trade-antibody-a06584-1-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-wb-testing-1.jpgAnti-POH1/PSMD14 Antibody Picoband® Western blot analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HT-1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POH1/PSMD14 antigen affinity purified polyclonal antibody (Catalog # A06584-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POH1/PSMD14 at approximately 36 kDa. The expected band size for POH1/PSMD14 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-2.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-3.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-4.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-5.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-6.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-7.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-8.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ihc-testing-9.jpgAnti-POH1/PSMD14 Antibody Picoband® IHC analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-if-testing-10.jpgAnti-POH1/PSMD14 Antibody Picoband® IF analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1). POH1/PSMD14 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POH1/PSMD14 Antibody (A06584-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-ip-testing-1.jpgAnti-POH1/PSMD14 Antibody Picoband®Immunoprecipitating (IP) POH1/PSMD14 in Hela whole cell lysate. Western blot analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (A06584-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-POH1/PSMD14 antibody in Hela whole cell lysate; Lane 3: anti-POH1/PSMD14 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POH1/PSMD14 antigen affinity purified polyclonal antibody (A06584-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for POH1/PSMD14 at approximately 35 kDa. The expected band size for POH1/PSMD14 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06584-1-psmd14-primary-antibodies-fcm-testing-11.pngAnti-POH1/PSMD14 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-PSMD14 antibody (A06584-1). Overlay histogram showing Raji cells stained with A06584-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD14 Antibody (A06584-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psmd2-picoband-trade-antibody-a06642-3-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06642-3-psmd2-primary-antibodies-wb-testing-1.jpgAnti-PSMD2 Antibody Picoband® Western blot analysis of PSMD2 using anti-PSMD2 antibody (A06642-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD2 antigen affinity purified polyclonal antibody (Catalog # A06642-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD2 at approximately 97 kDa. The expected band size for PSMD2 is at 97,100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06642-3-psmd2-primary-antibodies-if-testing-2.jpgAnti-PSMD2 Antibody Picoband® IF analysis of PSMD2 using anti-PSMD2 antibody (A06642-3). PSMD2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMD2 Antibody (A06642-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-psmd4-picoband-trade-antibody-a03544-1-boster.html2026-03-17T05:14:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-wb-testing-1.jpgAnti-PSMD4 Antibody Picoband® Western blot analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD4 antigen affinity purified polyclonal antibody (Catalog # A03544-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD4 at approximately 55 kDa. The expected band size for PSMD4 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-ihc-testing-2.jpgAnti-PSMD4 Antibody Picoband® IHC analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-ihc-testing-3.jpgAnti-PSMD4 Antibody Picoband® IHC analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-ihc-testing-4.jpgAnti-PSMD4 Antibody Picoband® IHC analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-ihc-testing-5.jpgAnti-PSMD4 Antibody Picoband® IHC analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-ihc-testing-6.jpgAnti-PSMD4 Antibody Picoband® IHC analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-ihc-testing-7.jpgAnti-PSMD4 Antibody Picoband® IHC analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-if-testing-8.jpgAnti-PSMD4 Antibody Picoband® IF analysis of PSMD4 using anti-PSMD4 antibody (A03544-1) and anti-Beta Tubulin antibody (M01857-3). PSMD4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMD4 Antibody (A03544-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-if-testing-9.jpgAnti-PSMD4 Antibody Picoband® IF analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-if-testing-10.jpgAnti-PSMD4 Antibody Picoband® IF analysis of PSMD4 using anti-PSMD4 antibody (A03544-1). PSMD4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD4 Antibody (A03544-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-ip-testing-1.jpgAnti-PSMD4 Antibody Picoband®Immunoprecipitating (IP) PSMD4 in Hela whole cell lysate. Western blot analysis of PSMD4 using anti-PSMD4 antibody (A03544-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PSMD4 antibody in Hela whole cell lysate; Lane 3: anti-PSMD4 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSMD4 antigen affinity purified polyclonal antibody (A03544-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PSMD4 at approximately 55 kDa. The expected band size for PSMD4 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03544-1-psmd4-primary-antibodies-fcm-testing-11.pngAnti-PSMD4 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-PSMD4 antibody (A03544-1). Overlay histogram showing Raji cells stained with A03544-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD4 Antibody (A03544-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psmd6-picoband-trade-antibody-a09338-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09338-1-psmd6-primary-antibodies-wb-testing-1.jpgAnti-PSMD6 Antibody Picoband® Western blot analysis of PSMD6 using anti-PSMD6 antibody (A09338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD6 antigen affinity purified polyclonal antibody (Catalog # A09338-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD6 at approximately 46 kDa. The expected band size for PSMD6 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09338-1-psmd6-primary-antibodies-if-testing-2.jpgAnti-PSMD6 Antibody Picoband® IF analysis of PSMD6 using anti-PSMD6 antibody (A09338-1) and anti-Beta Tubulin antibody (M01857-3). PSMD6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMD6 Antibody (A09338-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09338-1-psmd6-primary-antibodies-fcm-testing-3.pngAnti-PSMD6 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PSMD6 antibody (A09338-1). Overlay histogram showing JK cells stained with A09338-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD6 Antibody (A09338-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09338-1-psmd6-primary-antibodies-fcm-testing-4.pngAnti-PSMD6 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PSMD6 antibody (A09338-1). Overlay histogram showing MCF-7 cells stained with A09338-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD6 Antibody (A09338-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psmd7-picoband-trade-antibody-a09527-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09527-1-psmd7-primary-antibodies-wb-testing-1.jpgAnti-PSMD7 Antibody Picoband® Western blot analysis of PSMD7 using anti-PSMD7 antibody (A09527-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD7 antigen affinity purified polyclonal antibody (Catalog # A09527-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD7 at approximately 38 kDa. The expected band size for PSMD7 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09527-1-psmd7-primary-antibodies-fcm-testing-2.pngAnti-PSMD7 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PSMD7 antibody (A09527-1). Overlay histogram showing MCF-7 cells stained with A09527-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD7 Antibody (A09527-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-psme1-picoband-trade-antibody-a04638-2-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04638-2-psme1-primary-antibodies-wb-testing-1_1.jpgAnti-PSME1 Antibody Picoband®Western blot analysis of PSME1 using anti-PSME1 antibody (A04638-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat cells whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSME1 antigen affinity purified polyclonal antibody (A04638-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSME1 at approximately 29 kDa. The expected band size for PSME1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04638-2-psme1-primary-antibodies-ihc-testing-1.jpgAnti-PSME1 Antibody Picoband®IHC analysis of PSME1 using anti-PSME1 antibody (A04638-2). PSME1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSME1 Antibody (A04638-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04638-2-psme1-primary-antibodies-if-testing-1.jpgAnti-PSME1 Antibody Picoband®IF analysis of PSME1 using anti-PSME1 antibody (A04638-2). PSME1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSME1 Antibody (A04638-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04638-2-psme1-primary-antibodies-ip-testing-1.jpgAnti-PSME1 Antibody Picoband®Immunoprecipitating (IP) PSME1 in Jurkat whole cell lysate. Western blot analysis of PSME1 using anti-PSME1 antibody (A04638-2); Lane 1: Jurkat whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PSME1 antibody in Jurkat whole cell lysate; Lane 3: anti-PSME1 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSME1 antigen affinity purified polyclonal antibody (A04638-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PSME1 at approximately 29 kDa. The expected band size for PSME1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04638-2-psme1-primary-antibodies-fcm-testing-1.jpgAnti-PSME1 Antibody Picoband®Flow Cytometry analysis of Raji cells using anti-PSME1 antibody (A04638-2). Overlay histogram showing Raji cells stained with A04638-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSME1 Antibody (A04638-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-psmf1-picoband-trade-antibody-a08650-2-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08650-2-psmf1-primary-antibodies-wb-testing-1.jpgAnti-PSMF1 Antibody Picoband® Western blot analysis of PSMF1 using anti-PSMF1 antibody (A08650-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMF1 antigen affinity purified polyclonal antibody (Catalog # A08650-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMF1 at approximately 30 kDa. The expected band size for PSMF1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08650-2-psmf1-primary-antibodies-fcm-testing-2.pngAnti-PSMF1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PSMF1 antibody (A08650-2). Overlay histogram showing JK cells stained with A08650-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMF1 Antibody (A08650-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pxk-picoband-trade-antibody-a07555-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07555-1-pxk-primary-antibodies-wb-testing-1.jpgAnti-PXK Antibody Picoband® Western blot analysis of PXK using anti-PXK antibody (A07555-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXK antigen affinity purified polyclonal antibody (Catalog # A07555-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PXK at approximately 65 kDa. The expected band size for PXK is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07555-1-pxk-primary-antibodies-if-testing-2.jpgAnti-PXK Antibody Picoband® IF analysis of PXK using anti-PXK antibody (A07555-1). PXK was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PXK Antibody (A07555-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-asc-tms1-pycard-picoband-trade-antibody-a00362-6-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-pycard-primary-antibodies-wb-testing-1.jpgAnti-ASC/TMS1/PYCARD Antibody Picoband® Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-6) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-pycard-primary-antibodies-ihc-testing-2.jpgAnti-ASC/TMS1/PYCARD Antibody Picoband® IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human urothelial carcinoma with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-pycard-primary-antibodies-ihc-testing-3.jpgAnti-ASC/TMS1/PYCARD Antibody Picoband® IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-ihc-testing-4.pngAnti-ASC/TMS1/PYCARD Antibody Picoband® IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-ihc-testing-5.pngAnti-ASC/TMS1/PYCARD Antibody Picoband® IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-ihc-testing-6.pngAnti-ASC/TMS1/PYCARD Antibody Picoband® IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-if-testing-7.jpgAnti-ASC/TMS1/PYCARD Antibody Picoband® IF analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-if-testing-8.jpgAnti-ASC/TMS1/PYCARD Antibody Picoband® IF analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-if-testing-9.pngAnti-ASC/TMS1/PYCARD Antibody Picoband® IF analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-if-testing-10.pngAnti-ASC/TMS1/PYCARD Antibody Picoband® IF analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-if-testing-11.pngAnti-ASC/TMS1/PYCARD Antibody Picoband® IF analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00362-6-asc-primary-antibodies-fcm-testing-12.pngAnti-ASC/TMS1/PYCARD Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-ASC/TMS1/PYCARD antibody (A00362-6). Overlay histogram showing THP-1 cells stained with A00362-6 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (A00362-6, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pycr1-picoband-trade-antibody-a06018-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06018-1-pycr1-primary-antibodies-wb-testing-1.jpgAnti-PYCR1 Antibody Picoband® Western blot analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCR1 antigen affinity purified polyclonal antibody (Catalog # A06018-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCR1 at approximately 35 kDa. The expected band size for PYCR1 is at 33,35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06018-1-pycr1-primary-antibodies-ihc-testing-2.jpgAnti-PYCR1 Antibody Picoband® IHC analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). PYCR1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYCR1 Antibody (A06018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06018-1-pycr1-primary-antibodies-ihc-testing-3.jpgAnti-PYCR1 Antibody Picoband® IHC analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). PYCR1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYCR1 Antibody (A06018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06018-1-pycr1-primary-antibodies-if-testing-4.jpgAnti-PYCR1 Antibody Picoband® IF analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). PYCR1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PYCR1 Antibody (A06018-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06018-1-pycr1-primary-antibodies-if-testing-5.jpgAnti-PYCR1 Antibody Picoband® IF analysis of PYCR1 using anti-PYCR1 antibody (A06018-1). PYCR1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PYCR1 Antibody (A06018-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06018-1-pycr1-primary-antibodies-fcm-testing-6.pngAnti-PYCR1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PYCR1 antibody (A06018-1). Overlay histogram showing 293T cells stained with A06018-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYCR1 Antibody (A06018-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pycr2-picoband-trade-antibody-a10327-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10327-1-pycr2-primary-antibodies-wb-testing-1.jpgAnti-PYCR2 Antibody Picoband® Western blot analysis of PYCR2 using anti-PYCR2 antibody (A10327-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCR2 antigen affinity purified polyclonal antibody (Catalog # A10327-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCR2 at approximately 35 kDa. The expected band size for PYCR2 is at 34,35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10327-1-pycr2-primary-antibodies-if-testing-2.jpgAnti-PYCR2 Antibody Picoband® IF analysis of PYCR2 using anti-PYCR2 antibody (A10327-1). PYCR2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PYCR2 Antibody (A10327-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10327-1-pycr2-primary-antibodies-fcm-testing-3.pngAnti-PYCR2 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-PYCR2 antibody (A10327-1). Overlay histogram showing JK cells stained with A10327-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYCR2 Antibody (A10327-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-reep3-picoband-trade-antibody-a12243-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12243-1-reep3-primary-antibodies-wb-testing-1.jpgAnti-REEP3 Antibody Picoband® Western blot analysis of REEP3 using anti-REEP3 antibody (A12243-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REEP3 antigen affinity purified polyclonal antibody (Catalog # A12243-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REEP3 at approximately 34 kDa. The expected band size for REEP3 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12243-1-reep3-primary-antibodies-if-testing-2.jpgAnti-REEP3 Antibody Picoband® IF analysis of REEP3 using anti-REEP3 antibody (A12243-1). REEP3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-REEP3 Antibody (A12243-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-rxra-picoband-trade-antibody-a01299-2-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01299-2-rxra-primary-antibodies-wb-testing-1.jpgAnti-RXRA Antibody Picoband® Western blot analysis of RXRA using anti-RXRA antibody (A01299-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RXRA antigen affinity purified polyclonal antibody (Catalog # A01299-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RXRA at approximately 51 kDa. The expected band size for RXRA is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01299-2-rxra-primary-antibodies-fcm-testing-2.pngAnti-RXRA Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RXRA antibody (A01299-2). Overlay histogram showing Hela cells stained with A01299-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RXRA Antibody (A01299-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-tmem5-rxylt1-picoband-trade-antibody-a31815-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31815-1-rxytl1-primary-antibodies-wb-testing-1.jpgAnti-TMEM5/RXYLT1 Antibody Picoband® Western blot analysis of TMEM5/RXYLT1 using anti-TMEM5/RXYLT1 antibody (A31815-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM5/RXYLT1 antigen affinity purified polyclonal antibody (Catalog # A31815-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM5/RXYLT1 at approximately 56 kDa. The expected band size for TMEM5/RXYLT1 is at 45-51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31815-1-rxytl1-primary-antibodies-fcm-testing-2.pngAnti-TMEM5/RXYLT1 Antibody Picoband® Flow Cytometry analysis of Daudi cells using anti-TMEM5/RXYLT1 antibody (A31815-1). Overlay histogram showing Daudi cells stained with A31815-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM5/RXYLT1 Antibody (A31815-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-porin-vdac1-picoband-trade-antibody-a01168-1-boster.html2026-03-17T05:14:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-wb-testing-1.jpgAnti-Porin/VDAC1 Antibody Picoband® Western blot analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Porin/VDAC1 antigen affinity purified polyclonal antibody (Catalog # A01168-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Porin/VDAC1 at approximately 34 kDa. The expected band size for Porin/VDAC1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-2.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-3.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-4.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-5.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-6.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-7.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of human urothelial carcinoma with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-8.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-ihc-testing-9.jpgAnti-Porin/VDAC1 Antibody Picoband® IHC analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-if-testing-10.jpgAnti-Porin/VDAC1 Antibody Picoband® IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-if-testing-11.jpgAnti-Porin/VDAC1 Antibody Picoband® IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-if-testing-12.jpgAnti-Porin/VDAC1 Antibody Picoband® IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-if-testing-13.jpgAnti-Porin/VDAC1 Antibody Picoband® IF analysis of Porin/VDAC1 using anti-Porin/VDAC1 antibody (A01168-1). Porin/VDAC1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Porin/VDAC1 Antibody (A01168-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01168-1-vdac1-primary-antibodies-fcm-testing-14.pngAnti-Porin/VDAC1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-Porin/VDAC1 antibody (A01168-1). Overlay histogram showing Hela cells stained with A01168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Porin/VDAC1 Antibody (A01168-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-p21-cdkn1a-picoband-trade-antibody-a00145-1-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00145-1-p21-primary-anibodies-wb-testing-1.jpgAnti-P21/CDKN1A Antibody Picoband® Western blot analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P21/CDKN1A antigen affinity purified polyclonal antibody (Catalog # A00145-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P21/CDKN1A at approximately 21 kDa. The expected band size for P21/CDKN1A is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00145-1-p21-primary-anibodies-ihc-testing-2.jpgAnti-P21/CDKN1A Antibody Picoband® IHC analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1). P21/CDKN1A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P21/CDKN1A Antibody (A00145-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00145-1-p21-primary-anibodies-ihc-testing-3.jpgAnti-P21/CDKN1A Antibody Picoband® IHC analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1). P21/CDKN1A was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P21/CDKN1A Antibody (A00145-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00145-1-p21-primary-anibodies-if-testing-4.jpgAnti-P21/CDKN1A Antibody Picoband® IF analysis of P21/CDKN1A using anti-P21/CDKN1A antibody (A00145-1) and anti-Beta Tubulin antibody (M01857-3). P21/CDKN1A was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P21/CDKN1A Antibody (A00145-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-caspase-8-casp8-antibody-a00042-4-boster.html2026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-4-casp8-primary-antibodies-wb-testing-1.jpgAnti-Caspase-8 CASP8 AntibodyWB Validation in Human K562 Cells Loading: 15 μg of K562 cell lysate Antibodies: Casp8 A00042-4, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-4-casp8-primary-antibodies-ihc-testing-2.jpgAnti-Caspase-8 CASP8 AntibodyImmunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-4-casp8-primary-antibodies-if-testing-3.jpgAnti-Caspase-8 CASP8 AntibodyImmunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-4-casp8-primary-antibodies-icc-testing-4.jpgAnti-Caspase-8 CASP8 AntibodyImmunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-4-casp8-primary-antibodies-wb-testing-5.jpgAnti-Caspase-8 CASP8 AntibodyWB Validation in Mouse 3T3/NIH Cells Loading: 15 μg of 3T3/NIH cell lysate Antibodies: Casp8 A00042-4, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-4-casp8-primary-antibodies-if-testing-6.jpgAnti-Caspase-8 CASP8 AntibodyImmunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 μg/ml. Red: Caspase-8 Antibody (A00042-4) Blue: DAPI staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00042-4-casp8-primary-antibodies-wb-testing-7.jpgAnti-Caspase-8 CASP8 AntibodyWB Validation in Rat YB2/0 Cells Loading: 15 μg of YB2/0 cell lysate Antibodies: Casp8 A00042-4, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. https://www.bosterbio.com/catalog/product/view/id/1235122026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2224.pngHuman Jagged1 ELISA Kit PicoKine®Human Jagged1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235132026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2225.pngHuman CD49A ELISA Kit PicoKine®Human CD49A PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235142026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2226_2.pngHuman IL10RB ELISA Kit PicoKine®Human IL10RB PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235152026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2227.pngHuman Intergrin Alpha 5/CD49E ELISA Kit PicoKine®Human CD49E PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235162026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2228.pngHuman CD125 ELISA Kit PicoKine®Human CD125 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235172026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2229.jpgHuman Lipocalin-1 ELISA Kit PicoKine®Human Lipocalin PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235182026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2230.jpgHuman CD107a ELISA Kit PicoKine®Human CD107a PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235192026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2231.jpgHuman CD107b ELISA Kit PicoKine®Human CD107b PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235202026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2232.jpgMouse Layilin ELISA Kit PicoKine®Mouse Layilin PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235212026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2233.jpgHuman LRG1 ELISA Kit PicoKine®Human LRG1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235222026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2234.jpgHuman Galectin-8 ELISA Kit PicoKine®Human Galectin-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235232026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2235.pngHuman LECT2 ELISA Kit PicoKine®Human LECT2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235242026-03-10T04:35:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2236.pngHuman Lefty ELISA Kit PicoKine®Human Lefty PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235252026-03-10T04:35:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2237.pngHuman LRIG1 ELISA Kit PicoKine®Human LRIG1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235262026-03-10T04:35:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2238.jpgHuman LPL ELISA Kit PicoKine®Human LPL PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235282026-03-10T04:35:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2240.pngHuman LRRTM3 ELISA Kit PicoKine®Human LRRTM3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235292026-03-10T04:35:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2241.pngHuman LTK ELISA Kit PicoKine®Human LTK PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235302026-03-10T04:35:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0865.pngHuman IL-23 ELISA Kit PicoKine®Human IL-23 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235312026-03-10T04:35:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2242.pngHuman MARCO ELISA Kit PicoKine®Human MARCO PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1235322026-03-10T04:35:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2243.pngHuman MDGA1 ELISA Kit PicoKine®Human MDGA1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-prrc2c-picoband-trade-antibody-a11762-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11762-prrc2c-primary-antibodies-wb-testing-1.jpgAnti-PRRC2C Antibody Picoband® Western blot analysis of PRRC2C using anti-PRRC2C antibody (A11762). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRRC2C antigen affinity purified polyclonal antibody (Catalog # A11762) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRRC2C at approximately 290,94 kDa. The expected band size for PRRC2C is at 317 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11762-prrc2c-primary-antibodies-if-testing-2.jpgAnti-PRRC2C Antibody Picoband® IF analysis of PRRC2C using anti-PRRC2C antibody (A11762). PRRC2C was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRRC2C Antibody (A11762) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11762-prrc2c-primary-antibodies-fcm-testing-3.pngAnti-PRRC2C Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-PRRC2C antibody (A11762). Overlay histogram showing U251 cells stained with A11762 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRRC2C Antibody (A11762, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-angiotensinogen-agt-picoband-trade-antibody-a02103-4-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02103-4-agt-primary-antibodies-wb-testing-1.jpgAnti-Angiotensinogen/AGT Antibody Picoband® Western blot analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody (A02103-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HUH-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiotensinogen/AGT antigen affinity purified polyclonal antibody (Catalog # A02103-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiotensinogen/AGT at approximately 53 kDa. The expected band size for Angiotensinogen/AGT is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02103-4-agt-primary-antibodies-ihc-testing-2.jpgAnti-Angiotensinogen/AGT Antibody Picoband® IHC analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody (A02103-4). Angiotensinogen/AGT was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Angiotensinogen/AGT Antibody (A02103-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02103-4-agt-primary-antibodies-ihc-testing-3.jpgAnti-Angiotensinogen/AGT Antibody Picoband® IHC analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody (A02103-4). Angiotensinogen/AGT was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Angiotensinogen/AGT Antibody (A02103-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02103-4-agt-primary-antibodies-fcm-testing-4.pngAnti-Angiotensinogen/AGT Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-Angiotensinogen/AGT antibody (A02103-4). Overlay histogram showing HepG2 cells stained with A02103-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Angiotensinogen/AGT Antibody (A02103-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-kcnn4-picoband-trade-antibody-a01936-4-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01936-4-kcnn4-primary-antibodies-wb-testing-1.jpgAnti-Kcnn4 Antibody Picoband® Western blot analysis of Kcnn4 using anti-Kcnn4 antibody (A01936-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kcnn4 antigen affinity purified polyclonal antibody (Catalog # A01936-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kcnn4 at approximately 48 kDa. The expected band size for Kcnn4 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-fox2-rbm9-rbfox2-picoband-trade-antibody-a05389-2-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-wb-testing-1_1.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® Western blot analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOX2/RBM9/RBFOX2 antigen affinity purified polyclonal antibody (Catalog # A05389-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOX2/RBM9/RBFOX2 at approximately 60 kDa. The expected band size for FOX2/RBM9/RBFOX2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-ihc-testing-2_1.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-ihc-testing-3_1.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-ihc-testing-4_1.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-if-testing-8.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® IF analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-ihc-testing-5_1.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human cortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-fcm-testing-9.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). Overlay histogram showing SH-SY5Y cells stained with A05389-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-ihc-testing-6.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05389-2-rbfox2-primary-antibodies-ihc-testing-7.jpgAnti-FOX2/RBM9/RBFOX2 Antibody Picoband® IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-crabp1-picoband-trade-antibody-a07951-1-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07951-1-crabp1-primary-antibodies-wb-testing-1.jpgAnti-CRABP1 Antibody Picoband® Western blot analysis of CRABP1 using anti-CRABP1 antibody (A07951-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRABP1 antigen affinity purified polyclonal antibody (Catalog # A07951-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRABP1 at approximately 16 kDa. The expected band size for CRABP1 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07951-1-crabp1-primary-antibodies-fcm-testing-2.pngAnti-CRABP1 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-CRABP1 antibody (A07951-1). Overlay histogram showing SH-SY5Y cells stained with A07951-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRABP1 Antibody (A07951-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-e2f1-picoband-trade-antibody-a00257-3-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00257-3-e2f1-primary-antibodies-wb-testing-1.jpgAnti-E2F1 Antibody Picoband® Western blot analysis of E2F1 using anti-E2F1 antibody (A00257-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F1 antigen affinity purified polyclonal antibody (Catalog # A00257-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F1 at approximately 60 kDa. The expected band size for E2F1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00257-3-e2f1-primary-antibodies-fcm-testing-2.pngAnti-E2F1 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-E2F1 antibody (A00257-3). Overlay histogram showing Hela cells stained with A00257-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-E2F1 Antibody (A00257-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-c7orf47-ppp1r35-picoband-trade-antibody-a15208-1-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15208-1-ppp1r35-primary-antibodies-wb-testing-1.jpgAnti-C7orf47/PPP1R35 Antibody Picoband® Western blot analysis of C7orf47/PPP1R35 using anti-C7orf47/PPP1R35 antibody (A15208-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C7orf47/PPP1R35 antigen affinity purified polyclonal antibody (Catalog # A15208-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C7orf47/PPP1R35 at approximately 30 kDa. The expected band size for C7orf47/PPP1R35 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15208-1-ppp1r35-primary-antibodies-if-testing-2.jpgAnti-C7orf47/PPP1R35 Antibody Picoband® IF analysis of C7orf47/PPP1R35 using anti-C7orf47/PPP1R35 antibody (A15208-1) and anti-Beta Tubulin antibody (M01857-3). C7orf47/PPP1R35 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C7orf47/PPP1R35 Antibody (A15208-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15208-1-ppp1r35-primary-antibodies-fcm-testing-3.pngAnti-C7orf47/PPP1R35 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-C7orf47/PPP1R35 antibody (A15208-1). Overlay histogram showing K562 cells stained with A15208-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C7orf47/PPP1R35 Antibody (A15208-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prelp-picoband-trade-antibody-a07640-1-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07640-1-prelp-primary-antibodies-wb-testing-1.jpgAnti-PRELP Antibody Picoband® Western blot analysis of PRELP using anti-PRELP antibody (A07640-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRELP antigen affinity purified polyclonal antibody (Catalog # A07640-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRELP at approximately 65 kDa. The expected band size for PRELP is at 44,65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07640-1-prelp-primary-antibodies-fcm-testing-2.pngAnti-PRELP Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-PRELP antibody (A07640-1). Overlay histogram showing Hela cells stained with A07640-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRELP Antibody (A07640-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07640-1-prelp-primary-antibodies-fcm-testing-3.pngAnti-PRELP Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-PRELP antibody (A07640-1). Overlay histogram showing RT4 cells stained with A07640-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRELP Antibody (A07640-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prex1-picoband-trade-antibody-a03098-2-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03098-2-prex1-primary-antibodies-wb-testing-1.jpgAnti-PREX1 Antibody Picoband® Western blot analysis of PREX1 using anti-PREX1 antibody (A03098-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX1 antigen affinity purified polyclonal antibody (Catalog # A03098-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX1 at approximately 186 kDa. The expected band size for PREX1 is at 186 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03098-2-prex1-primary-antibodies-fcm-testing-2.pngAnti-PREX1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PREX1 antibody (A03098-2). Overlay histogram showing MCF-7 cells stained with A03098-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PREX1 Antibody (A03098-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nanp-picoband-trade-antibody-a09658-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-wb-testing-1.jpgAnti-NANP Antibody Picoband® Western blot analysis of NANP using anti-NANP antibody (A09658). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANP antigen affinity purified polyclonal antibody (Catalog # A09658) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NANP at approximately 30 kDa. The expected band size for NANP is at 28,30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-ihc-testing-2.jpgAnti-NANP Antibody Picoband® IHC analysis of NANP using anti-NANP antibody (A09658). NANP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANP Antibody (A09658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-ihc-testing-3.jpgAnti-NANP Antibody Picoband® IHC analysis of NANP using anti-NANP antibody (A09658). NANP was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANP Antibody (A09658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-ihc-testing-4.jpgAnti-NANP Antibody Picoband® IHC analysis of NANP using anti-NANP antibody (A09658). NANP was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANP Antibody (A09658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-ihc-testing-5.jpgAnti-NANP Antibody Picoband® IHC analysis of NANP using anti-NANP antibody (A09658). NANP was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANP Antibody (A09658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-ihc-testing-6.jpgAnti-NANP Antibody Picoband® IHC analysis of NANP using anti-NANP antibody (A09658). NANP was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANP Antibody (A09658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-ihc-testing-7.jpgAnti-NANP Antibody Picoband® IHC analysis of NANP using anti-NANP antibody (A09658). NANP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANP Antibody (A09658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-ihc-testing-8.jpgAnti-NANP Antibody Picoband® IHC analysis of NANP using anti-NANP antibody (A09658). NANP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANP Antibody (A09658) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09658-nanp-primary-antibodies-fcm-testing-9.pngAnti-NANP Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-NANP antibody (A09658). Overlay histogram showing HepG2 cells stained with A09658 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NANP Antibody (A09658, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ncor2-picoband-trade-antibody-a01412-2-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-2-ncor2-primary-antibodies-wb-testing-1.jpgAnti-NCOR2 Antibody Picoband® Western blot analysis of NCOR2 using anti-NCOR2 antibody (A01412-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOR2 antigen affinity purified polyclonal antibody (Catalog # A01412-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOR2 at approximately 275 kDa. The expected band size for NCOR2 is at 275 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-2-ncor2-primary-antibodies-if-testing-2.jpgAnti-NCOR2 Antibody Picoband® IF analysis of NCOR2 using anti-NCOR2 antibody (A01412-2) and anti-Beta Tubulin antibody (M01857-3). NCOR2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NCOR2 Antibody (A01412-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-2-ncor2-primary-antibodies-fcm-testing-3.pngAnti-NCOR2 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-NCOR2 antibody (A01412-2). Overlay histogram showing Hela cells stained with A01412-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOR2 Antibody (A01412-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-2-ncor2-primary-antibodies-fcm-testing-4.pngAnti-NCOR2 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-NCOR2 antibody (A01412-2). Overlay histogram showing RT4 cells stained with A01412-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOR2 Antibody (A01412-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ndrg4-picoband-trade-antibody-a04972-1-boster.html2026-03-17T05:14:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04972-1-ndrg4-primary-antibodies-wb-testing-1.jpgAnti-NDRG4 Antibody Picoband® Western blot analysis of NDRG4 using anti-NDRG4 antibody (A04972-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG4 antigen affinity purified polyclonal antibody (Catalog # A04972-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG4 at approximately 37 kDa. The expected band size for NDRG4 is at 37-45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04972-1-ndrg4-primary-antibodies-if-testing-2.jpgAnti-NDRG4 Antibody Picoband® IF analysis of NDRG4 using anti-NDRG4 antibody (A04972-1) and anti-Beta Tubulin antibody (M01857-3). NDRG4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDRG4 Antibody (A04972-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04972-1-ndrg4-primary-antibodies-fcm-testing-3.pngAnti-NDRG4 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-NDRG4 antibody (A04972-1). Overlay histogram showing 293T cells stained with A04972-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDRG4 Antibody (A04972-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nedd1-picoband-trade-antibody-a06427-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06427-2-nedd1-primary-antibodies-wb-testing-1.jpgAnti-NEDD1 Antibody Picoband® Western blot analysis of NEDD1 using anti-NEDD1 antibody (A06427-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD1 antigen affinity purified polyclonal antibody (Catalog # A06427-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD1 at approximately 70 kDa. The expected band size for NEDD1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06427-2-nedd1-primary-antibodies-fcm-testing-2.pngAnti-NEDD1 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-NEDD1 antibody (A06427-2). Overlay histogram showing SH-SY5Y cells stained with A06427-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD1 Antibody (A06427-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-negr1-picoband-trade-antibody-a06730-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06730-2-negr1-primary-antibodies-wb-testing-1.jpgAnti-NEGR1 Antibody Picoband® Western blot analysis of NEGR1 using anti-NEGR1 antibody (A06730-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEGR1 antigen affinity purified polyclonal antibody (Catalog # A06730-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEGR1 at approximately 46 kDa. The expected band size for NEGR1 is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-neil3-picoband-trade-antibody-a05497-4-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05497-4-neil3-primary-antibodies-wb-testing-1.jpgAnti-NEIL3 Antibody Picoband® Western blot analysis of NEIL3 using anti-NEIL3 antibody (A05497-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEIL3 antigen affinity purified polyclonal antibody (Catalog # A05497-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEIL3 at approximately 70 kDa. The expected band size for NEIL3 is at 62-68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05497-4-neil3-primary-antibodies-ihc-testing-2.jpgAnti-NEIL3 Antibody Picoband® IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4). NEIL3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEIL3 Antibody (A05497-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05497-4-neil3-primary-antibodies-ihc-testing-3.jpgAnti-NEIL3 Antibody Picoband® IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4). NEIL3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEIL3 Antibody (A05497-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05497-4-neil3-primary-antibodies-ihc-testing-4.jpgAnti-NEIL3 Antibody Picoband® IHC analysis of NEIL3 using anti-NEIL3 antibody (A05497-4). NEIL3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEIL3 Antibody (A05497-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ppm1j-picoband-trade-antibody-a15780-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15780-2-ppm1j-primary-antibodies-wb-testing-1.jpgAnti-PPM1J Antibody Picoband® Western blot analysis of PPM1J using anti-PPM1J antibody (A15780-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1J antigen affinity purified polyclonal antibody (Catalog # A15780-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1J at approximately 55 kDa. The expected band size for PPM1J is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15780-2-ppm1j-primary-antibodies-if-testing-2.jpgAnti-PPM1J Antibody Picoband® IF analysis of PPM1J using anti-PPM1J antibody (A15780-2). PPM1J was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPM1J Antibody (A15780-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-prrc1-picoband-trade-antibody-a15083-1-boster.html2026-03-25T05:24:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15083-1-prrc1-primary-antibodies-wb-testing-1.jpgAnti-PRRC1 Antibody Picoband® Western blot analysis of PRRC1 using anti-PRRC1 antibody (A15083-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRRC1 antigen affinity purified polyclonal antibody (Catalog # A15083-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRRC1 at approximately 47 kDa. The expected band size for PRRC1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15083-1-prrc1-primary-antibodies-if-testing-2.jpgAnti-PRRC1 Antibody Picoband® IF analysis of PRRC1 using anti-PRRC1 antibody (A15083-1). PRRC1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRRC1 Antibody (A15083-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15083-1-prrc1-primary-antibodies-fcm-testing-3.pngAnti-PRRC1 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-PRRC1 antibody (A15083-1). Overlay histogram showing U251 cells stained with A15083-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRRC1 Antibody (A15083-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-rpb5-polr2e-picoband-trade-antibody-a08765-1-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08765-1-polr2e-primary-antibodies-wb-testing-1.jpgAnti-RPB5/POLR2E Antibody Picoband® Western blot analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody (A08765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPB5/POLR2E antigen affinity purified polyclonal antibody (Catalog # A08765-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPB5/POLR2E at approximately 23 kDa. The expected band size for RPB5/POLR2E is at 23-25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08765-1-polr2e-primary-antibodies-if-testing-2.jpgAnti-RPB5/POLR2E Antibody Picoband® IF analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody (A08765-1) and anti-Beta Tubulin antibody (M01857-3). RPB5/POLR2E was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPB5/POLR2E Antibody (A08765-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08765-1-polr2e-primary-antibodies-fcm-testing-3.pngAnti-RPB5/POLR2E Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-RPB5/POLR2E antibody (A08765-1). Overlay histogram showing Hela cells stained with A08765-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPB5/POLR2E Antibody (A08765-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppil2-picoband-trade-antibody-a11451-1-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11451-1-ppil2-primary-antibodies-wb-testing-1.jpgAnti-PPIL2 Antibody Picoband® Western blot analysis of PPIL2 using anti-PPIL2 antibody (A11451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIL2 antigen affinity purified polyclonal antibody (Catalog # A11451-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPIL2 at approximately 59 kDa. The expected band size for PPIL2 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11451-1-ppil2-primary-antibodies-if-testing-2.jpgAnti-PPIL2 Antibody Picoband® IF analysis of PPIL2 using anti-PPIL2 antibody (A11451-1) and anti-Beta Tubulin antibody (M01857-3). PPIL2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPIL2 Antibody (A11451-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11451-1-ppil2-primary-antibodies-fcm-testing-3.pngAnti-PPIL2 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PPIL2 antibody (A11451-1). Overlay histogram showing 293T cells stained with A11451-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPIL2 Antibody (A11451-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp1r15b-picoband-trade-antibody-a10225-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10225-2-ppp1r15b-primary-antibodies-wb-testing-1.jpgAnti-PPP1R15B Antibody Picoband® Western blot analysis of PPP1R15B using anti-PPP1R15B antibody (A10225-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R15B antigen affinity purified polyclonal antibody (Catalog # A10225-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R15B at approximately 110 kDa. The expected band size for PPP1R15B is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10225-2-ppp1r15b-primary-antibodies-fcm-testing-2.pngAnti-PPP1R15B Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PPP1R15B antibody (A10225-2). Overlay histogram showing 293T cells stained with A10225-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R15B Antibody (A10225-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp2r5a-picoband-trade-antibody-a05935-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05935-2-ppp2r5a-primary-antibodies-wb-testing-1.jpgAnti-PPP2R5A Antibody Picoband® Western blot analysis of PPP2R5A using anti-PPP2R5A antibody (A05935-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5A antigen affinity purified polyclonal antibody (Catalog # A05935-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R5A at approximately 56 kDa. The expected band size for PPP2R5A is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05935-2-ppp2r5a-primary-antibodies-if-testing-2.jpgAnti-PPP2R5A Antibody Picoband® IF analysis of PPP2R5A using anti-PPP2R5A antibody (A05935-2) and anti-Beta Tubulin antibody (M01857-3). PPP2R5A was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP2R5A Antibody (A05935-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-ppp3cb-picoband-trade-antibody-a07303-1-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07303-1-ppp3cb-primary-antibodies-wb-testing-1.jpgAnti-PPP3CB Antibody Picoband® Western blot analysis of PPP3CB using anti-PPP3CB antibody (A07303-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CB antigen affinity purified polyclonal antibody (Catalog # A07303-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3CB at approximately 59 kDa. The expected band size for PPP3CB is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07303-1-ppp3cb-primary-antibodies-ihc-testing-2.jpgAnti-PPP3CB Antibody Picoband® IHC analysis of PPP3CB using anti-PPP3CB antibody (A07303-1). PPP3CB was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3CB Antibody (A07303-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07303-1-ppp3cb-primary-antibodies-ihc-testing-3.jpgAnti-PPP3CB Antibody Picoband® IHC analysis of PPP3CB using anti-PPP3CB antibody (A07303-1). PPP3CB was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3CB Antibody (A07303-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07303-1-ppp3cb-primary-antibodies-ihc-testing-4.jpgAnti-PPP3CB Antibody Picoband® IHC analysis of PPP3CB using anti-PPP3CB antibody (A07303-1). PPP3CB was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3CB Antibody (A07303-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07303-1-ppp3cb-primary-antibodies-fcm-testing-5_1.pngAnti-PPP3CB Antibody Picoband® Flow Cytometry analysis of U937 cells using anti-PPP3CB antibody (A07303-1). Overlay histogram showing U937 cells stained with A07303-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP3CB Antibody (A07303-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-peroxiredoxin-2-prdx2-picoband-trade-antibody-a01982-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01982-2-prdx2-primary-antibodies-wb-testing-1.jpgAnti-peroxiredoxin 2/PRDX2 Antibody Picoband® Western blot analysis of Peroxiredoxin 2/PRDX2 using anti-Peroxiredoxin 2/PRDX2 antibody (A01982-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 2/PRDX2 antigen affinity purified polyclonal antibody (Catalog # A01982-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 2/PRDX2 at approximately 22 kDa. The expected band size for Peroxiredoxin 2/PRDX2 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01982-2-prdx2-primary-antibodies-fcm-testing-2.pngAnti-peroxiredoxin 2/PRDX2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-Peroxiredoxin 2/PRDX2 antibody (A01982-2). Overlay histogram showing U251 cells stained with A01982-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 2/PRDX2 Antibody (A01982-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prolactin-prl-picoband-trade-antibody-a00601-1-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00601-1-prl-primary-antibodies-wb-testing-1.jpgAnti-Prolactin/PRL Antibody Picoband® Western blot analysis of Prolactin/PRL using anti-Prolactin/PRL antibody (A00601-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolactin/PRL antigen affinity purified polyclonal antibody (Catalog # A00601-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolactin/PRL at approximately 26 kDa. The expected band size for Prolactin/PRL is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ndufa8-picoband-trade-antibody-a10547-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10547-2-ndufa8-primary-antibodies-wb-testing-1.jpgAnti-NDUFA8 Antibody Picoband® Western blot analysis of NDUFA8 using anti-NDUFA8 antibody (A10547-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA8 antigen affinity purified polyclonal antibody (Catalog # A10547-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA8 at approximately 19 kDa. The expected band size for NDUFA8 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10547-2-ndufa8-primary-antibodies-fcm-testing-2.pngAnti-NDUFA8 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-NDUFA8 antibody (A10547-2). Overlay histogram showing 293T cells stained with A10547-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA8 Antibody (A10547-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10547-2-ndufa8-primary-antibodies-fcm-testing-3.pngAnti-NDUFA8 Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-NDUFA8 antibody (A10547-2). Overlay histogram showing U87 cells stained with A10547-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA8 Antibody (A10547-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prpf4-picoband-trade-antibody-a08225-2-boster.html2026-03-17T05:14:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08225-2-prpf4-primary-antibodies-wb-testing-1_1.jpgAnti-PRPF4 Antibody Picoband® Western blot analysis of PRPF4 using anti-PRPF4 antibody (A08225-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Siha whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF4 antigen affinity purified polyclonal antibody (A08225-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRPF4 at approximately 58 kDa. The expected band size for PRPF4 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08225-2-prpf4-primary-antibodies-ihc-testing-2.jpgAnti-PRPF4 Antibody Picoband® IHC analysis of PRPF4 using anti-PRPF4 antibody (A08225-2). PRPF4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF4 Antibody (A08225-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08225-2-prpf4-primary-antibodies-ihc-testing-3.jpgAnti-PRPF4 Antibody Picoband® IHC analysis of PRPF4 using anti-PRPF4 antibody (A08225-2). PRPF4 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF4 Antibody (A08225-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08225-2-prpf4-primary-antibodies-ihc-testing-4.jpgAnti-PRPF4 Antibody Picoband® IHC analysis of PRPF4 using anti-PRPF4 antibody (A08225-2). PRPF4 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF4 Antibody (A08225-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08225-2-prpf4-primary-antibodies-ihc-testing-5.jpgAnti-PRPF4 Antibody Picoband® IHC analysis of PRPF4 using anti-PRPF4 antibody (A08225-2). PRPF4 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRPF4 Antibody (A08225-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08225-2-prpf4-primary-antibodies-if-testing-6.jpgAnti-PRPF4 Antibody Picoband® IF analysis of RPS28 using anti-RPS28 antibody (A08225-2) and anti-Tubulin Alpha antibody (M03989-3). RPS28 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS28 Antibody (A08225-2) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08225-2-prpf4-primary-antibodies-fcm-testing-7.pngAnti-PRPF4 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PRPF4 antibody (A08225-2). Overlay histogram showing SiHa cells stained with A08225-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF4 Antibody (A04887-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-prr5-picoband-trade-antibody-a05975-1-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05975-1-prr5-primary-antibodies-wb-testing-1.jpgAnti-PRR5 Antibody Picoband® Western blot analysis of PRR5 using anti-PRR5 antibody (A05975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRR5 antigen affinity purified polyclonal antibody (Catalog # A05975-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRR5 at approximately 39 kDa. The expected band size for PRR5 is at 33,43,50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05975-1-prr5-primary-antibodies-fcm-testing-2.pngAnti-PRR5 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PRR5 antibody (A05975-1). Overlay histogram showing 293T cells stained with A05975-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRR5 Antibody (A05975-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-n4bp1-picoband-trade-antibody-a12575-3-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12575-3-n4bp1-primary-antibodies-wb-testing-1.jpgAnti-N4BP1 Antibody Picoband® Western blot analysis of N4BP1 using anti-N4BP1 antibody (A12575-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N4BP1 antigen affinity purified polyclonal antibody (Catalog # A12575-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for N4BP1 at approximately 110 kDa. The expected band size for N4BP1 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12575-3-n4bp1-primary-antibodies-if-testing-2.jpgAnti-N4BP1 Antibody Picoband® IF analysis of N4BP1 using anti-N4BP1 antibody (A12575-3). N4BP1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-N4BP1 Antibody (A12575-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12575-3-n4bp1-primary-antibodies-fcm-testing-3.pngAnti-N4BP1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-N4BP1 antibody (A12575-3). Overlay histogram showing JK cells stained with A12575-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-N4BP1 Antibody (A12575-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-grp78-bip-hspa5-picoband-trade-antibody-a00955-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00955-hspa5-primary-antibodies-wb-testing-1.jpgAnti-GRP78/BIP/HSPA5 Antibody Picoband® Western blot analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (A00955). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP78/BIP/HSPA5 antigen affinity purified polyclonal antibody (Catalog # A00955) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP78/BIP/HSPA5 at approximately 78 kDa. The expected band size for GRP78/BIP/HSPA5 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00955-12931_2024_2986_fig2_html.pngAnti-GRP78/BIP/HSPA5 Antibody Picoband®Over-expression of circSSR1 inhibited vascular smooth muscle cell pyroptosis. A , Over-expression of circSSR1 reduce reactive oxygen species probes (DCFH-DA, green) staining in various groups ( n = 6). B , Endoplasmic reticulum stress (ERS) related protein ATF6, IRE1 and GRP78 expression. C , LDH cytotoxicity assay kit (LDH) was used to detect LDH release ( n = 6). D , Western blot to demonstrate pyroptosis related protein NLRP3, GSDMD and Caspase-1 expression ( n = 5). E , Fluorescence staining of IL-1β in PASMCs under hypoxia ( n = 6). F , Over-expression of circSSR1 plasmid decreased PI positive cells ( n = 5). Scale bars, 50 μm. All values are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12931-024-02986-w'>39354535</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00955-12931_2024_2986_fig4_html.pngAnti-GRP78/BIP/HSPA5 Antibody Picoband®CircSSR1 regulated pyroptosis and endoplasmic stress through acting on SSR1 expression under hypoxia. A and B , qRT-PCR and western blot showed over-expression of circSSR1 had no significance on mRNA ( n = 6) but affect SSR1 translation ( n = 4). C , DAVID website demonstrated Gene Ontology (GO) analysis showed SSR1 was related with unfold protein response. D , Endoplasmic reticulum specific probe (Green) and SSR1 (Red) can localization proved by ER-Tracker. E , Interfere with SSR1 under hypoxia can prevent hypoxia-induced LDH release ( n = 6). F , Transfection siRNA decrease immunofluorescence intensity of IL-1β in HYP. G , Pyroptosis markable proteins expression after transfection siSSR1 ( n = 5). H , PI staining showed the positive cells after transfect siRNA ( n = 6). I , Western blot showed ER stress marker proteins expression after transfected with siSSR1 ( n = 5–6). J , Immunofluorescence intensity of GRP78 decreased after transfect siRNA ( n = 6). Scale bars, 50 μm. All values are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12931-024-02986-w'>39354535</a> https://www.bosterbio.com/products/primary-antibodies/anti-pbld-picoband-trade-antibody-a10149-2-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10149-2-pbld-primary-antibodies-wb-testing-1.jpgAnti-PBLD Antibody Picoband® Western blot analysis of PBLD using anti-PBLD antibody (A10149-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBLD antigen affinity purified polyclonal antibody (Catalog # A10149-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBLD at approximately 32 kDa. The expected band size for PBLD is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10149-2-pbld-primary-antibodies-fcm-testing-2.pngAnti-PBLD Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-PBLD antibody (A10149-2). Overlay histogram showing SiHa cells stained with A10149-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PBLD Antibody (A10149-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-mbl2-picoband-trade-antibody-a01000-5-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01000-5-mbl2-primary-antibodies-wb-testing-1.jpgAnti-MBL2 Antibody Picoband® Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-5) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01000-5-mbl2-primary-antibodies-fcm-testing-2.pngAnti-MBL2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-MBL2 antibody (A01000-5). Overlay histogram showing HepG2 cells stained with A01000-5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MBL2 Antibody (A01000-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-gclm-picoband-trade-antibody-a02948-2-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02948-2-gclm-primary-antibodies-wb-testing-1.jpgAnti-GCLM Antibody Picoband® Western blot analysis of GCLM using anti-GCLM antibody (A02948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # A02948-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLM at approximately 31 kDa. The expected band size for GCLM is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-cep164-picoband-trade-antibody-a05971-1-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05971-1-cep164-primary-antibodies-wb-testing-1.jpgAnti-CEP164 Antibody Picoband® Western blot analysis of CEP164 using anti-CEP164 antibody (A05971-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEP164 antigen affinity purified polyclonal antibody (Catalog # A05971-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEP164 at approximately 164 kDa. The expected band size for CEP164 is at 164 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05971-1-cep164-primary-antibodies-if-testing-2.jpgAnti-CEP164 Antibody Picoband® IF analysis of CEP164 using anti-CEP164 antibody (A05971-1). CEP164 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CEP164 Antibody (A05971-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05971-1-cep164-primary-antibodies-fcm-testing-3.pngAnti-CEP164 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-CEP164 antibody (A05971-1). Overlay histogram showing K562 cells stained with A05971-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEP164 Antibody (A05971-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05971-1-cep164-primary-antibodies-fcm-testing-4.pngAnti-CEP164 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-CEP164 antibody (A05971-1). Overlay histogram showing RT4 cells stained with A05971-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEP164 Antibody (A05971-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-narg1-naa15-picoband-trade-antibody-a05636-3-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05636-3-naa15-primary-antibodies-wb-testing-1.jpgAnti-NARG1/NAA15 Antibody Picoband® Western blot analysis of NARG1/NAA15 using anti-NARG1/NAA15 antibody (A05636-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NARG1/NAA15 antigen affinity purified polyclonal antibody (Catalog # A05636-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NARG1/NAA15 at approximately 100 kDa. The expected band size for NARG1/NAA15 is at 100,101 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-twf1-picoband-trade-antibody-a07004-1-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07004-1-twf1-primary-antibodies-wb-testing-1.jpgAnti-TWF1 Antibody Picoband® Western blot analysis of TWF1 using anti-TWF1 antibody (A07004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWF1 antigen affinity purified polyclonal antibody (Catalog # A07004-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWF1 at approximately 42 kDa. The expected band size for TWF1 is at 40,42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07004-1-twf1-primary-antibodies-fcm-testing-2.pngAnti-TWF1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-TWF1 antibody (A07004-1). Overlay histogram showing MCF-7 cells stained with A07004-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TWF1 Antibody (A07004-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-trnt1-picoband-trade-antibody-a06030-1-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06030-1-trnt1-primary-antibodies-wb-testing-1_1.jpgAnti-TRNT1 Antibody Picoband® Western blot analysis of TRNT1 using anti-TRNT1 antibody (A06030-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNT1 antigen affinity purified polyclonal antibody (Catalog # A06030-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNT1 at approximately 50 kDa. The expected band size for TRNT1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-2_1.jpgAnti-TRNT1 Antibody Picoband® IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1). TRNT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-3_1.jpgAnti-TRNT1 Antibody Picoband® IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1). TRNT1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-4_1.jpgAnti-TRNT1 Antibody Picoband® IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1). TRNT1 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06030-1-trnt1-primary-antibodies-ihc-testing-5_1.jpgAnti-TRNT1 Antibody Picoband® IHC analysis of TRNT1 using anti-TRNT1 antibody (A06030-1). TRNT1 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRNT1 Antibody (A06030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ttc9-picoband-trade-antibody-a14174-boster.html2026-03-17T05:14:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14174-ttc9-primary-antibodies-wb-testing-1.jpgAnti-TTC9 Antibody Picoband® Western blot analysis of TTC9 using anti-TTC9 antibody (A14174). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC9 antigen affinity purified polyclonal antibody (Catalog # A14174) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC9 at approximately 27 kDa. The expected band size for TTC9 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14174-ttc9-primary-antibodies-if-testing-2.jpgAnti-TTC9 Antibody Picoband® IF analysis of TTC9 using anti-TTC9 antibody (A14174). TTC9 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TTC9 Antibody (A14174) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14174-ttc9-primary-antibodies-fcm-testing-3.pngAnti-TTC9 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-TTC9 antibody (A14174). Overlay histogram showing JK cells stained with A14174 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC9 Antibody (A14174, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14174-ttc9-primary-antibodies-fcm-testing-4.pngAnti-TTC9 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-TTC9 antibody (A14174). Overlay histogram showing RT4 cells stained with A14174 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC9 Antibody (A14174, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ttc29-picoband-trade-antibody-a17783-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17783-1-ttc29-primary-antibodies-wb-testing-1.jpgAnti-TTC29 Antibody Picoband® Western blot analysis of TTC29 using anti-TTC29 antibody (A17783-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC29 antigen affinity purified polyclonal antibody (Catalog # A17783-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC29 at approximately 55 kDa. The expected band size for TTC29 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17783-1-ttc29-primary-antibodies-fcm-testing-2.pngAnti-TTC29 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-TTC29 antibody (A17783-1). Overlay histogram showing SH-SY5Y cells stained with A17783-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC29 Antibody (A17783-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ttc38-picoband-trade-antibody-a16794-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16794-1-ttc38-primary-antibodies-wb-testing-1.jpgAnti-TTC38 Antibody Picoband® Western blot analysis of TTC38 using anti-TTC38 antibody (A16794-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC38 antigen affinity purified polyclonal antibody (Catalog # A16794-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC38 at approximately 53 kDa. The expected band size for TTC38 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16794-1-ttc38-primary-antibodies-if-testing-2.jpgAnti-TTC38 Antibody Picoband® IF analysis of TTC38 using anti-TTC38 antibody (A16794-1). TTC38 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TTC38 Antibody (A16794-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16794-1-ttc38-primary-antibodies-fcm-testing-3.pngAnti-TTC38 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-TTC38 antibody (A16794-1). Overlay histogram showing HepG2 cells stained with A16794-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC38 Antibody (A16794-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ttc39a-picoband-trade-antibody-a16108-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16108-ttc39a-primary-antibodies-wb-testing-1.jpgAnti-TTC39A Antibody Picoband® Western blot analysis of TTC39A using anti-TTC39A antibody (A16108). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC39A antigen affinity purified polyclonal antibody (Catalog # A16108) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC39A at approximately 72 kDa. The expected band size for TTC39A is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16108-ttc39a-primary-antibodies-fcm-testing-2.pngAnti-TTC39A Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-TTC39A antibody (A16108). Overlay histogram showing 293T cells stained with A16108 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC39A Antibody (A16108, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-twf2-picoband-trade-antibody-a09748-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09748-1-tef2-primary-antibodies-wb-testing-1.jpgAnti-TWF2 Antibody Picoband® Western blot analysis of TWF2 using anti-TWF2 antibody (A09748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWF2 antigen affinity purified polyclonal antibody (Catalog # A09748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWF2 at approximately 40 kDa. The expected band size for TWF2 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09748-1-tef2-primary-antibodies-if-testing-2.jpgAnti-TWF2 Antibody Picoband® IF analysis of TWF2 using anti-TWF2 antibody (A09748-1). TWF2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TWF2 Antibody (A09748-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09748-1-tef2-primary-antibodies-fcm-testing-3.pngAnti-TWF2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-TWF2 antibody (A09748-1). Overlay histogram showing HepG2 cells stained with A09748-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TWF2 Antibody (A09748-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-txndc17-picoband-trade-antibody-a09772-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09772-1-txndc17-primary-antibodies-wb-testing-1.jpgAnti-TXNDC17 Antibody Picoband® Western blot analysis of TXNDC17 using anti-TXNDC17 antibody (A09772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNDC17 antigen affinity purified polyclonal antibody (Catalog # A09772-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNDC17 at approximately 14 kDa. The expected band size for TXNDC17 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09772-1-txndc17-primary-antibodies-fcm-testing-2.pngAnti-TXNDC17 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-TXNDC17 antibody (A09772-1). Overlay histogram showing JK cells stained with A09772-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TXNDC17 Antibody (A09772-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-samd1-picoband-trade-antibody-a14386-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14386-1-samd1-primary-antibodies-wb-testing-1.jpgAnti-SAMD1 Antibody Picoband® Western blot analysis of SAMD1 using anti-SAMD1 antibody (A14386-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAMD1 antigen affinity purified polyclonal antibody (Catalog # A14386-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAMD1 at approximately 72 kDa. The expected band size for SAMD1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14386-1-41598_2025_97627_fig6_html.pngAnti-SAMD1 Antibody Picoband®Effect of treatment with cyclopamine on the BMP pathway in BMPR2 knockdown hPASMCs. The hPASMCs were treated with Lv-NC, Lv-siBMPR2, and SHH in the presence or absence of cyclopamine (2.5, 5, or 10 µM) for 48 h. Exogenous BMP4 protein (10 ng/ml) was added to the culture medium to promote proliferation. ( a ) Western blot analysis and quantification showing the protein expression levels of p-SMAD1/5/8 and SMAD1/5/8 in the various groups. The protein expression levels were normalized to GAPDH. The mRNA expression levels of ( b ) ID1 and ( c ) ID3 detected by real-time PCR analysis are shown for the various groups. The mRNA expression levels were normalized to GAPDH . ( d ) Western blot analysis of SHH protein expression levels in the various groups. ( e ) Western blot analysis and quantification shows the p-SAMD1/5/8 and SMAD1/5/8 protein expression levels in the various groups. The protein expression levels were normalized to GAPDH. ( f ) Real-time PCR analysis of ID1 and ID3 mRNA expression levels in the various groups. The mRNA expression levels were normalized to GAPDH . ( n = 3 for each treatment group). # P < 0.05 vs. the control group; * P < 0.05 vs. the Lv-siBMPR2 group; ** P < 0.01 vs. the Lv-siBMPR2 group. BMPR bone morphogenetic protein receptor, Cy cyclopamine, hPASMCs human pulmonary arterial smooth muscle cells, Lv lentivirus, SHH Sonic hedgehog. Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-025-97627-7'>40216933</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14386-1-samd1-primary-antibodies-if-testing-2.jpgAnti-SAMD1 Antibody Picoband® IF analysis of SAMD1 using anti-SAMD1 antibody (A14386-1). SAMD1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SAMD1 Antibody (A14386-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14386-1-samd1-primary-antibodies-fcm-testing-3.jpgAnti-SAMD1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SAMD1 antibody (A14386-1). Overlay histogram showing K562 cells stained with A14386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAMD1 Antibody (A14386-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-scfd2-picoband-trade-antibody-a15482-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15482-1-scfd2-primary-antibodies-wb-testing-1.jpgAnti-SCFD2 Antibody Picoband® Western blot analysis of SCFD2 using anti-SCFD2 antibody (A15482-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCFD2 antigen affinity purified polyclonal antibody (Catalog # A15482-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCFD2 at approximately 75 kDa. The expected band size for SCFD2 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15482-1-scfd2-primary-antibodies-if-testing-2.jpgAnti-SCFD2 Antibody Picoband® IF analysis of SCFD2 using anti-SCFD2 antibody (A15482-1). SCFD2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCFD2 Antibody (A15482-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15482-1-scfd2-primary-antibodies-fcm-testing-3.pngAnti-SCFD2 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-SCFD2 antibody (A15482-1). Overlay histogram showing SH-SY5Y cells stained with A15482-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SCFD2 Antibody (A15482-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-scly-picoband-trade-antibody-a09046-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-wb-testing-1.jpgAnti-SCLY Antibody Picoband® Western blot analysis of SCLY using anti-SCLY antibody (A09046). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCLY antigen affinity purified polyclonal antibody (Catalog # A09046) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCLY at approximately 48 kDa. The expected band size for SCLY is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-ihc-testing-2.jpgAnti-SCLY Antibody Picoband® IHC analysis of SCLY using anti-SCLY antibody (A09046). SCLY was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCLY Antibody (A09046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-ihc-testing-3.jpgAnti-SCLY Antibody Picoband® IHC analysis of SCLY using anti-SCLY antibody (A09046). SCLY was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCLY Antibody (A09046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-ihc-testing-4.jpgAnti-SCLY Antibody Picoband® IHC analysis of SCLY using anti-SCLY antibody (A09046). SCLY was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCLY Antibody (A09046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-ihc-testing-5.jpgAnti-SCLY Antibody Picoband® IHC analysis of SCLY using anti-SCLY antibody (A09046). SCLY was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCLY Antibody (A09046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-ihc-testing-6.jpgAnti-SCLY Antibody Picoband® IHC analysis of SCLY using anti-SCLY antibody (A09046). SCLY was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCLY Antibody (A09046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-ihc-testing-7.jpgAnti-SCLY Antibody Picoband® IHC analysis of SCLY using anti-SCLY antibody (A09046). SCLY was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCLY Antibody (A09046) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09046-scly-primary-antibodies-fcm-testing-8.pngAnti-SCLY Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-SCLY antibody (A09046). Overlay histogram showing SH-SY5Y cells stained with A09046 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCLY Antibody (A09046, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sema3d-picoband-trade-antibody-a09239-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09239-1-sema3d-primary-antibodies-wb-testing-1.jpgAnti-SEMA3D Antibody Picoband® Western blot analysis of SEMA3D using anti-SEMA3D antibody (A09239-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3D antigen affinity purified polyclonal antibody (Catalog # A09239-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3D at approximately 90 kDa. The expected band size for SEMA3D is at 88,90 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sema4a-picoband-trade-antibody-a06779-3-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06779-3-sema4a-primary-antibodies-wb-testing-1.jpgAnti-SEMA4A Antibody Picoband® Western blot analysis of SEMA4A using anti-SEMA4A antibody (A06779-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse ANA-1 tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA4A antigen affinity purified polyclonal antibody (Catalog # A06779-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA4A at approximately 105 kDa. The expected band size for SEMA4A is at 84,105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06779-3-sema4a-primary-antibodies-fcm-testing-2.pngAnti-SEMA4A Antibody Picoband® Flow Cytometry analysis of JK cells using anti-SEMA4A antibody (A06779-3). Overlay histogram showing JK cells stained with A06779-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SEMA4A Antibody (A06779-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-3bp2-sh3bp2-picoband-trade-antibody-a04008-1-boster.html2026-03-17T05:14:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04008-1-sh3bp2-primary-antibodies-wb-testing-1.jpgAnti-3BP2/SH3BP2 Antibody Picoband® Western blot analysis of 3BP2/SH3BP2 using anti-3BP2/SH3BP2 antibody (A04008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-3BP2/SH3BP2 antigen affinity purified polyclonal antibody (Catalog # A04008-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 3BP2/SH3BP2 at approximately 70 kDa. The expected band size for 3BP2/SH3BP2 is at 62,70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04008-1-sh3bp2-primary-antibodies-fcm-testing-2.pngAnti-3BP2/SH3BP2 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-3BP2/SH3BP2 antibody (A04008-1). Overlay histogram showing JK cells stained with A04008-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-3BP2/SH3BP2 Antibody (A04008-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp2r3a-picoband-trade-antibody-a11620-1-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11620-1-ppp2r3a-primary-antibodies-wb-testing-1.jpgAnti-PPP2R3A Antibody Picoband® Western blot analysis of PPP2R3A using anti-PPP2R3A antibody (A11620-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R3A antigen affinity purified polyclonal antibody (Catalog # A11620-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R3A at approximately 128 kDa. The expected band size for PPP2R3A is at 48,61,130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11620-1-ppp2r3a-primary-antibodies-if-testing-2.jpgAnti-PPP2R3A Antibody Picoband® IF analysis of PPP2R3A using anti-PPP2R3A antibody (A11620-1). PPP2R3A was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP2R3A Antibody (A11620-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11620-1-ppp2r3a-primary-antibodies-fcm-testing-3.pngAnti-PPP2R3A Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-PPP2R3A antibody (A11620-1). Overlay histogram showing SH-SY5Y cells stained with A11620-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R3A Antibody (A11620-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppwd1-picoband-trade-antibody-a11798-1-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11798-1-ppwd1-primary-antibodies-wb-testing-1.jpgAnti-PPWD1 Antibody Picoband® Western blot analysis of PPWD1 using anti-PPWD1 antibody (A11798-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPWD1 antigen affinity purified polyclonal antibody (Catalog # A11798-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPWD1 at approximately 70 kDa. The expected band size for PPWD1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11798-1-ppwd1-primary-antibodies-ihc-testing-2.jpgAnti-PPWD1 Antibody Picoband® IHC analysis of PPWD1 using anti-PPWD1 antibody (A11798-1). PPWD1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPWD1 Antibody (A11798-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11798-1-ppwd1-primary-antibodies-ihc-testing-3.jpgAnti-PPWD1 Antibody Picoband® IHC analysis of PPWD1 using anti-PPWD1 antibody (A11798-1). PPWD1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPWD1 Antibody (A11798-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11798-1-ppwd1-primary-antibodies-if-testing-4.jpgAnti-PPWD1 Antibody Picoband® IF analysis of PPWD1 using anti-PPWD1 antibody (A11798-1). PPWD1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPWD1 Antibody (A11798-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11798-1-ppwd1-primary-antibodies-fcm-testing-5.pngAnti-PPWD1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PPWD1 antibody (A11798-1). Overlay histogram showing 293T cells stained with A11798-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPWD1 Antibody (A11798-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prdm4-picoband-trade-antibody-a11843-2-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11843-2-prdm4-primary-antibodies-wb-testing-1.jpgAnti-PRDM4 Antibody Picoband® Western blot analysis of PRDM4 using anti-PRDM4 antibody (A11843-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO320 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM4 antigen affinity purified polyclonal antibody (Catalog # A11843-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM4 at approximately 88 kDa. The expected band size for PRDM4 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11843-2-prdm4-primary-antibodies-if-testing-2.jpgAnti-PRDM4 Antibody Picoband® IF analysis of PRDM4 using anti-PRDM4 antibody (A11843-2). PRDM4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRDM4 Antibody (A11843-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-prune1-picoband-trade-antibody-a31798-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31798-prune1-primary-antibodies-wb-testing-1.jpgAnti-PRUNE1 Antibody Picoband® Western blot analysis of PRUNE1 using anti-PRUNE1 antibody (A31798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRUNE1 antigen affinity purified polyclonal antibody (Catalog # A31798) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRUNE1 at approximately 60 kDa. The expected band size for PRUNE1 is at 50,60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31798-prune1-primary-antibodies-if-testing-2.jpgAnti-PRUNE1 Antibody Picoband® IF analysis of PRUNE1 using anti-PRUNE1 antibody (A31798). PRUNE1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRUNE1 Antibody (A31798) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31798-prune1-primary-antibodies-fcm-testing-3.pngAnti-PRUNE1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PRUNE1 antibody (A31798). Overlay histogram showing 293T cells stained with A31798 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRUNE1 Antibody (A31798, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-pygl-picoband-trade-antibody-a06317-1-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06317-1-pygl-primary-antibodies-wb-testing-1_1.jpgAnti-PYGL Antibody Picoband® Western blot analysis of PYGL using anti-PYGL antibody (A06317-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYGL antigen affinity purified polyclonal antibody (Catalog # A06317-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PYGL at approximately 97 kDa. The expected band size for PYGL is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06317-1-pygl-primary-antibodies-ihc-testing-2.jpgAnti-PYGL Antibody Picoband® IHC analysis of PYGL using anti-PYGL antibody (A06317-1). PYGL was detected in a paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGL Antibody (A06317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06317-1-pygl-primary-antibodies-ihc-testing-3.jpgAnti-PYGL Antibody Picoband® IHC analysis of PYGL using anti-PYGL antibody (A06317-1). PYGL was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGL Antibody (A06317-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06317-1-pygl-primary-antibodies-fcm-testing-4.jpgAnti-PYGL Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PYGL antibody (A06317-1). Overlay histogram showing A431 cells stained with A06317-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYGL Antibody (A06317-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-pyroxd1-picoband-trade-antibody-a17214-4-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17214-4-pyroxd1-primary-antibodies-wb-testing-1.jpgAnti-PYROXD1 Antibody Picoband® Western blot analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYROXD1 antigen affinity purified polyclonal antibody (Catalog # A17214-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYROXD1 at approximately 65 kDa. The expected band size for PYROXD1 is at 50,56,72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17214-4-pyroxd1-primary-antibodies-if-testing-2.jpgAnti-PYROXD1 Antibody Picoband® IF analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-4). PYROXD1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PYROXD1 Antibody (A17214-4) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17214-4-pyroxd1-primary-antibodies-fcm-testing-3.pngAnti-PYROXD1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PYROXD1 antibody (A17214-4). Overlay histogram showing 293T cells stained with A17214-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYROXD1 Antibody (A17214-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-prtn3-picoband-trade-antibody-a03863-3-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03863-3-prtn3-primary-antibodies-wb-testing-1.jpgAnti-PRTN3 Antibody Picoband® Western blot analysis of PRTN3 using anti-PRTN3 antibody (A03863-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRTN3 antigen affinity purified polyclonal antibody (Catalog # A03863-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRTN3 at approximately 32 kDa. The expected band size for PRTN3 is at 28,32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03863-3-prtn3-primary-antibodies-ihc-testing-2.jpgAnti-PRTN3 Antibody Picoband® IHC analysis of PRTN3 using anti-PRTN3 antibody (A03863-3). PRTN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRTN3 Antibody (A03863-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03863-3-prtn3-primary-antibodies-ihc-testing-3.jpgAnti-PRTN3 Antibody Picoband® IHC analysis of PRTN3 using anti-PRTN3 antibody (A03863-3). PRTN3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRTN3 Antibody (A03863-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03863-3-prtn3-primary-antibodies-if-testing-1.jpgAnti-PRTN3 Antibody Picoband®IF analysis of PRTN3 using anti-PRTN3 antibody (A03863-3). PRTN3 was detected in an immunocytochemical section of THP-1 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRTN3 Antibody (A03863-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03863-3-prtn3-primary-antibodies-fcm-testing-4.pngAnti-PRTN3 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-PRTN3 antibody (A03863-3). Overlay histogram showing THP-1 cells stained with A03863-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRTN3 Antibody (A03863-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-serpinb9-picoband-trade-antibody-a04734-1-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04734-1-serpinb9-primary-antibodies-wb-testing-1.jpgAnti-SERPINB9 Antibody Picoband® Western blot analysis of SERPINB9 using anti-SERPINB9 antibody (A04734-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB9 antigen affinity purified polyclonal antibody (Catalog # A04734-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB9 at approximately 38 kDa. The expected band size for SERPINB9 is at 38,42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04734-1-serpinb9-primary-antibodies-if-testing-2.jpgAnti-SERPINB9 Antibody Picoband® IF analysis of SERPINB9 using anti-SERPINB9 antibody (A04734-1). SERPINB9 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SERPINB9 Antibody (A04734-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04734-1-serpinb9-primary-antibodies-fcm-testing-3.pngAnti-SERPINB9 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-SERPINB9 antibody (A04734-1). Overlay histogram showing K562 cells stained with A04734-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB9 Antibody (A04734-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-serpind1-picoband-trade-antibody-a04607-3-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04607-3-serpind1-primary-antibodies-wb-testing-1.jpgAnti-SERPIND1 Antibody Picoband® Western blot analysis of SERPIND1 using anti-SERPIND1 antibody (A04607-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPIND1 antigen affinity purified polyclonal antibody (Catalog # A04607-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPIND1 at approximately 70 kDa. The expected band size for SERPIND1 is at 57-60,66-75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04607-3-serpind1-primary-antibodies-fcm-testing-2.pngAnti-SERPIND1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SERPIND1 antibody (A04607-3). Overlay histogram showing 293T cells stained with A04607-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPIND1 Antibody (A04607-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sesn1-picoband-trade-antibody-a05559-2-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05559-2-sesn1-primary-antibodies-wb-testing-1.jpgAnti-SESN1 Antibody Picoband® Western blot analysis of SESN1 using anti-SESN1 antibody (A05559-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SESN1 antigen affinity purified polyclonal antibody (Catalog # A05559-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SESN1 at approximately 56 kDa. The expected band size for SESN1 is at 56,65-75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05559-2-sesn1-primary-antibodies-if-testing-2.jpgAnti-SESN1 Antibody Picoband® IF analysis of SESN1 using anti-SESN1 antibody (A05559-2) and anti-Beta Tubulin antibody (M01857-3). SESN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SESN1 Antibody (A05559-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05559-2-sesn1-primary-antibodies-fcm-testing-3.pngAnti-SESN1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-SESN1 antibody (A05559-2). Overlay histogram showing 293T cells stained with A05559-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SESN1 Antibody (A05559-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sf3a2-picoband-trade-antibody-a11289-2-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-wb-testing-1.jpgAnti-SF3A2 Antibody Picoband® Western blot analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A2 antigen affinity purified polyclonal antibody (Catalog # A11289-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3A2 at approximately 66 kDa. The expected band size for SF3A2 is at 49,60,66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ihc-testing-2.jpgAnti-SF3A2 Antibody Picoband® IHC analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). SF3A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A2 Antibody (A11289-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ihc-testing-3.jpgAnti-SF3A2 Antibody Picoband® IHC analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). SF3A2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A2 Antibody (A11289-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ihc-testing-4.jpgAnti-SF3A2 Antibody Picoband® IHC analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). SF3A2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A2 Antibody (A11289-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ihc-testing-5.jpgAnti-SF3A2 Antibody Picoband® IHC analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). SF3A2 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A2 Antibody (A11289-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ihc-testing-6.jpgAnti-SF3A2 Antibody Picoband® IHC analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). SF3A2 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A2 Antibody (A11289-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ihc-testing-7.jpgAnti-SF3A2 Antibody Picoband® IHC analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). SF3A2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A2 Antibody (A11289-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ihc-testing-8.jpgAnti-SF3A2 Antibody Picoband® IHC analysis of SF3A2 using anti-SF3A2 antibody (A11289-2). SF3A2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A2 Antibody (A11289-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-if-testing-9.jpgAnti-SF3A2 Antibody Picoband® IF analysis of SF3A2 using anti-SF3A2 antibody (A11289-2) and anti-Beta Tubulin antibody (M01857-3). SF3A2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SF3A2 Antibody (A11289-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-ip-testing-1.jpgAnti-SF3A2 Antibody Picoband®Immunoprecipitating (IP) SF3A2 in SH-SY5Y whole cell lysate. Western blot analysis of SF3A2 using anti-SF3A2 antibody (A11289-2); Lane 1: SH-SY5Y whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SF3A2 antibody in SH-SY5Y whole cell lysate; Lane 3: anti-SF3A2 antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SF3A2 antigen affinity purified polyclonal antibody (A11289-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SF3A2 at approximately 66 kDa. The expected band size for SF3A2 is at 49,60,66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11289-2-sf3a2-primary-antibodies-fcm-testing-10.pngAnti-SF3A2 Antibody Picoband® Flow Cytometry analysis of SiHa cells using anti-SF3A2 antibody (A11289-2). Overlay histogram showing SiHa cells stained with A11289-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SF3A2 Antibody (A11289-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-rfc4-picoband-trade-antibody-a07702-1-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07702-1-rcf4-primary-antibodies-wb-testing-1.jpgAnti-RFC4 Antibody Picoband® Western blot analysis of RFC4 using anti-RFC4 antibody (A07702-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC4 antigen affinity purified polyclonal antibody (Catalog # A07702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC4 at approximately 37 kDa. The expected band size for RFC4 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07702-1-rcf4-primary-antibodies-if-testing-2.jpgAnti-RFC4 Antibody Picoband® IF analysis of RFC4 using anti-RFC4 antibody (A07702-1) and anti-Beta Tubulin antibody (M01857-3). RFC4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RFC4 Antibody (A07702-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07702-1-rcf4-primary-antibodies-fcm-testing-3.pngAnti-RFC4 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-RFC4 antibody (A07702-1). Overlay histogram showing Raji cells stained with A07702-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFC4 Antibody (A07702-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-xpa-picoband-trade-antibody-a01182-4-boster.html2026-03-17T05:14:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01182-4-xpa-primary-antibodies-wb-testing-1_1.jpgAnti-XPA Antibody Picoband® Western blot analysis of XPA using anti-XPA antibody (A01182-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPA antigen affinity purified polyclonal antibody (Catalog # A01182-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPA at approximately 39 kDa. The expected band size for XPA is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01182-4-xpa-primary-antibodies-if-testing-2_1.jpgAnti-XPA Antibody Picoband® IF analysis of XPA using anti-XPA antibody (A01182-4) and anti-Beta Tubulin antibody (M01857-3). XPA was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-XPA Antibody (A01182-4) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01182-4-xpa-primary-antibodies-fcm-testing-3.jpgAnti-XPA Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-XPA antibody (A01182-4). Overlay histogram showing 293T cells stained with A01182-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XPA Antibody (A01182-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-rph3a-picoband-trade-antibody-a10247-2-boster.html2026-03-17T05:14:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10247-2-rph3a-primary-antibodies-wb-testing-1.jpgAnti-RPH3A Antibody Picoband® Western blot analysis of RPH3A using anti-RPH3A antibody (A10247-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPH3A antigen affinity purified polyclonal antibody (Catalog # A10247-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPH3A at approximately 85 kDa. The expected band size for RPH3A is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10247-2-rph3a-primary-antibodies-ihc-testing-2.jpgAnti-RPH3A Antibody Picoband® IHC analysis of RPH3A using anti-RPH3A antibody (A10247-2). RPH3A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPH3A Antibody (A10247-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10247-2-rph3a-primary-antibodies-ihc-testing-3.jpgAnti-RPH3A Antibody Picoband® IHC analysis of RPH3A using anti-RPH3A antibody (A10247-2). RPH3A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPH3A Antibody (A10247-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-robo4-picoband-trade-antibody-a04479-2-boster.html2026-03-17T05:14:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04479-2-robo4-primary-antibodies-wb-testing-1.jpgAnti-ROBO4 Antibody Picoband® Western blot analysis of ROBO4 using anti-ROBO4 antibody (A04479-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse LLC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO4 antigen affinity purified polyclonal antibody (Catalog # A04479-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO4 at approximately 107 kDa. The expected band size for ROBO4 is at 90-170 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-pfkfb1-picoband-trade-antibody-a08398-2-boster.html2026-03-17T05:14:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08398-2-pfkfb1-primary-antibodies-wb-testing-1.jpgAnti-PFKFB1 Antibody Picoband® Western blot analysis of PFKFB1 using anti-PFKFB1 antibody (A08398-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB1 antigen affinity purified polyclonal antibody (Catalog # A08398-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKFB1 at approximately 55 kDa. The expected band size for PFKFB1 is at 47,55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08398-2-pfkfb1-primary-antibodies-ihc-testing-2.jpgAnti-PFKFB1 Antibody Picoband® IHC analysis of PFKFB1 using anti-PFKFB1 antibody (A08398-2). PFKFB1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PFKFB1 Antibody (A08398-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08398-2-pfkfb1-primary-antibodies-ihc-testing-3.jpgAnti-PFKFB1 Antibody Picoband® IHC analysis of PFKFB1 using anti-PFKFB1 antibody (A08398-2). PFKFB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PFKFB1 Antibody (A08398-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08398-2-pfkfb1-primary-antibodies-ihc-testing-4.jpgAnti-PFKFB1 Antibody Picoband® IHC analysis of PFKFB1 using anti-PFKFB1 antibody (A08398-2). PFKFB1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PFKFB1 Antibody (A08398-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08398-2-pfkfb1-primary-antibodies-ihc-testing-5.jpgAnti-PFKFB1 Antibody Picoband® IHC analysis of PFKFB1 using anti-PFKFB1 antibody (A08398-2). PFKFB1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PFKFB1 Antibody (A08398-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08398-2-pfkfb1-primary-antibodies-fcm-testing-6.pngAnti-PFKFB1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PFKFB1 antibody (A08398-2). Overlay histogram showing HepG2 cells stained with A08398-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKFB1 Antibody (A08398-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-polr2d-picoband-trade-antibody-a12839-1-boster.html2026-03-17T05:14:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12839-1-polr2d-primary-antibodies-wb-testing-1_1.jpgAnti-POLR2D Antibody Picoband®Western blot analysis of POLR2D using anti-POLR2D antibody (A12839-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2D antigen affinity purified polyclonal antibody (Catalog # A12839-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLR2D at approximately 18 kDa. The expected band size for POLR2D is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-ephb3-picoband-trade-antibody-a04659-boster.html2026-03-17T05:14:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04659-ephb3-primary-antibodies-wb-testing-1.jpgAnti-EPHB3 Antibody Picoband® Western blot analysis of EPHB3 using anti-EPHB3 antibody (A04659). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB3 antigen affinity purified polyclonal antibody (Catalog # A04659) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHB3 at approximately 110 kDa. The expected band size for EPHB3 is at 110-130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04659-ephb3-primary-antibodies-ihc-testing-1.jpgAnti-EPHB3 Antibody Picoband®IHC analysis of EPHB3 using anti-EPHB3 antibody (A04659). EPHB3 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPHB3 Antibody (A04659) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04659-ephb3-primary-antibodies-ihc-testing-2.jpgAnti-EPHB3 Antibody Picoband®IHC analysis of EPHB3 using anti-EPHB3 antibody (A04659). EPHB3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPHB3 Antibody (A04659) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-ppfia2-picoband-trade-antibody-a11773-boster.html2026-03-17T05:14:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11773-ppfia2-primary-antibodies-wb-testing-1.jpgAnti-PPFIA2 Antibody Picoband® Western blot analysis of PPFIA2 using anti-PPFIA2 antibody (A11773). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPFIA2 antigen affinity purified polyclonal antibody (Catalog # A11773) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPFIA2 at approximately 143 kDa. The expected band size for PPFIA2 is at 143 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11773-ppfia2-primary-antibodies-ihc-testing-3.jpgAnti-PPFIA2 Antibody Picoband®IHC analysis of PPFIA2 using anti-PPFIA2 antibody (A11773). PPFIA2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPFIA2 Antibody (A11773) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11773-ppfia2-primary-antibodies-ihc-testing-2.jpgAnti-PPFIA2 Antibody Picoband® IHC analysis of PPFIA2 using anti-PPFIA2 antibody (A11773). PPFIA2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPFIA2 Antibody (A11773) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11773-ppfia2-primary-antibodies-fcm-testing-3.pngAnti-PPFIA2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-PPFIA2 antibody (A11773). Overlay histogram showing U251 cells stained with A11773 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPFIA2 Antibody (A11773, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppm1f-picoband-trade-antibody-a07776-1-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07776-1-ppm1f-primary-antibodies-wb-testing-1_1.jpgAnti-PPM1F Antibody Picoband®Western blot analysis of PPM1F using anti-PPM1F antibody (A07776-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1F antigen affinity purified polyclonal antibody (Catalog # A07776-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPM1F at approximately 50 kDa. The expected band size for PPM1F is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07776-1-ppm1f-primary-antibodies-if-testing-1.jpgAnti-PPM1F Antibody Picoband®IF analysis of PPM1F using anti-PPM1F antibody (A07776-1) and anti-Tubulin Alpha antibody (M03989-3). PPM1F was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPM1F Antibody (A07776-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1032) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07776-1-ppm1f-primary-antibodies-if-testing-2_1.jpgAnti-PPM1F Antibody Picoband®IF analysis of PPM1F using anti-PPM1F antibody (AA07776-1). PPM1F was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PPM1F Antibody (A07776-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07776-1-ppm1f-primary-antibodies-fcm-testing-1.jpgAnti-PPM1F Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PPM1F antibody (A07776-1). Overlay histogram showing K562 cells stained with A07776-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1F Antibody (A07776-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp1r3b-picoband-trade-antibody-a09697-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09697-ppp1r3b-primary-antibodies-wb-testing-1.jpgAnti-PPP1R3B Antibody Picoband® Western blot analysis of PPP1R3B using anti-PPP1R3B antibody (A09697). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R3B antigen affinity purified polyclonal antibody (Catalog # A09697) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R3B at approximately 33 kDa. The expected band size for PPP1R3B is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-prdm2-picoband-trade-antibody-a03223-2-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03223-2-prdm2-primary-antibodies-wb-testing-1.jpgAnti-PRDM2 Antibody Picoband® Western blot analysis of PRDM2 using anti-PRDM2 antibody (A03223-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM2 antigen affinity purified polyclonal antibody (Catalog # A03223-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM2 at approximately 280 kDa. The expected band size for PRDM2 is at 189,250-280 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03223-2-prdm2-primary-antibodies-ihc-testing-2.jpgAnti-PRDM2 Antibody Picoband® IHC analysis of PRDM2 using anti-PRDM2 antibody (A03223-2). PRDM2 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM2 Antibody (A03223-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03223-2-prdm2-primary-antibodies-ihc-testing-3.jpgAnti-PRDM2 Antibody Picoband® IHC analysis of PRDM2 using anti-PRDM2 antibody (A03223-2). PRDM2 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM2 Antibody (A03223-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03223-2-prdm2-primary-antibodies-ihc-testing-4.jpgAnti-PRDM2 Antibody Picoband® IHC analysis of PRDM2 using anti-PRDM2 antibody (A03223-2). PRDM2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM2 Antibody (A03223-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03223-2-prdm2-primary-antibodies-ihc-testing-5.jpgAnti-PRDM2 Antibody Picoband® IHC analysis of PRDM2 using anti-PRDM2 antibody (A03223-2). PRDM2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM2 Antibody (A03223-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03223-2-prdm2-primary-antibodies-if-testing-6.jpgAnti-PRDM2 Antibody Picoband® IF analysis of PRDM2 using anti-PRDM2 antibody (A03223-2) and anti-Beta Tubulin antibody (M01857-3). PRDM2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRDM2 Antibody (A03223-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03223-2-prdm2-primary-antibodies-fcm-testing-7.pngAnti-PRDM2 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PRDM2 antibody (A03223-2). Overlay histogram showing HepG2 cells stained with A03223-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM2 Antibody (A03223-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-kepi-ppp1r14c-picoband-trade-antibody-a10973-2-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10973-2-ppp1r14c-primary-antibodies-wb-testing-1.jpgAnti-KEPI/PPP1R14C Antibody Picoband® Western blot analysis of KEPI/PPP1R14C using anti-KEPI/PPP1R14C antibody (A10973-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat H9C2(2-1) whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KEPI/PPP1R14C antigen affinity purified polyclonal antibody (Catalog # A10973-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KEPI/PPP1R14C at approximately 20 kDa. The expected band size for KEPI/PPP1R14C is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10973-2-ppp1r14c-primary-antibodies-fcm-testing-2.pngAnti-KEPI/PPP1R14C Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-KEPI/PPP1R14C antibody (A10973-2). Overlay histogram showing 293T cells stained with A10973-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEPI/PPP1R14C Antibody (A10973-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ppp3r1-picoband-trade-antibody-a06469-1-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06469-1-ppp3r1-primary-antibodies-wb-testing-1.jpgAnti-PPP3R1 Antibody Picoband® Western blot analysis of PPP3R1 using anti-PPP3R1 antibody (A06469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3R1 antigen affinity purified polyclonal antibody (Catalog # A06469-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3R1 at approximately 15 kDa. The expected band size for PPP3R1 is at 15,19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06469-1-ppp3r1-primary-antibodies-ihc-testing-2.jpgAnti-PPP3R1 Antibody Picoband® IHC analysis of PPP3R1 using anti-PPP3R1 antibody (A06469-1). PPP3R1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3R1 Antibody (A06469-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06469-1-ppp3r1-primary-antibodies-ihc-testing-3.jpgAnti-PPP3R1 Antibody Picoband® IHC analysis of PPP3R1 using anti-PPP3R1 antibody (A06469-1). PPP3R1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3R1 Antibody (A06469-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06469-1-ppp3r1-primary-antibodies-fcm-testing-4.pngAnti-PPP3R1 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-PPP3R1 antibody (A06469-1). Overlay histogram showing Caco-2 cells stained with A06469-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP3R1 Antibody (A06469-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ndufa4-picoband-trade-antibody-a09276-3-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09276-3-ndufa4-primary-antibodies-wb-testing-1.jpgAnti-NDUFA4 Antibody Picoband® Western blot analysis of NDUFA4 using anti-NDUFA4 antibody (A09276-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T98G whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA4 antigen affinity purified polyclonal antibody (Catalog # A09276-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA4 at approximately 12 kDa. The expected band size for NDUFA4 is at 9 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09276-3-ndufa4-primary-antibodies-ihc-testing-2.jpgAnti-NDUFA4 Antibody Picoband® IHC analysis of NDUFA4 using anti-NDUFA4 antibody (A09276-3). NDUFA4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA4 Antibody (A09276-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09276-3-ndufa4-primary-antibodies-ihc-testing-3.jpgAnti-NDUFA4 Antibody Picoband® IHC analysis of NDUFA4 using anti-NDUFA4 antibody (A09276-3). NDUFA4 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA4 Antibody (A09276-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09276-3-ndufa4-primary-antibodies-ihc-testing-4.jpgAnti-NDUFA4 Antibody Picoband® IHC analysis of NDUFA4 using anti-NDUFA4 antibody (A09276-3). NDUFA4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA4 Antibody (A09276-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09276-3-ndufa4-primary-antibodies-if-testing-5.jpgAnti-NDUFA4 Antibody Picoband® IF analysis of NDUFA4 using anti-NDUFA4 antibody (A09276-3). NDUFA4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDUFA4 Antibody (A09276-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09276-3-ndufa4-primary-antibodies-fcm-testing-6.pngAnti-NDUFA4 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-NDUFA4 antibody (A09276-3). Overlay histogram showing K562 cells stained with A09276-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA4 Antibody (A09276-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nbr1-picoband-trade-antibody-a03534-1-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03534-1-nbr1-primary-antibodies-wb-testing-1.jpgAnti-NBR1 Antibody Picoband® Western blot analysis of NBR1 using anti-NBR1 antibody (A03534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NBR1 antigen affinity purified polyclonal antibody (Catalog # A03534-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NBR1 at approximately 140 kDa. The expected band size for NBR1 is at 107,140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03534-1-nbr1-primary-antibodies-if-testing-2.jpgAnti-NBR1 Antibody Picoband® IF analysis of NBR1 using anti-NBR1 antibody (A03534-1) and anti-Beta Tubulin antibody (M01857-3). NBR1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NBR1 Antibody (A03534-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03534-1-nbr1-primary-antibodies-fcm-testing-3.pngAnti-NBR1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-NBR1 antibody (A03534-1). Overlay histogram showing MCF-7 cells stained with A03534-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NBR1 Antibody (A03534-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ncaph-picoband-trade-antibody-a09559-3-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09559-3-ncaph-primary-antibodies-wb-testing-1.jpgAnti-NCAPH Antibody Picoband® Western blot analysis of NCAPH using anti-NCAPH antibody (A09559-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAPH antigen affinity purified polyclonal antibody (Catalog # A09559-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAPH at approximately 100 kDa. The expected band size for NCAPH is at 83-100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09559-3-ncaph-primary-antibodies-if-testing-2.jpgAnti-NCAPH Antibody Picoband® IF analysis of NCAPH using anti-NCAPH antibody (A09559-3) and anti-Beta Tubulin antibody (M01857-3). NCAPH was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NCAPH Antibody (A09559-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09559-3-ncaph-primary-antibodies-fcm-testing-3.pngAnti-NCAPH Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-NCAPH antibody (A09559-3). Overlay histogram showing 293T cells stained with A09559-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCAPH Antibody (A09559-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-ncbp1-picoband-trade-antibody-a07424-3-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-wb-testing-1.jpgAnti-NCBP1 Antibody Picoband® Western blot analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCBP1 antigen affinity purified polyclonal antibody (Catalog # A07424-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCBP1 at approximately 92 kDa. The expected band size for NCBP1 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-2.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-3.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-4.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-5_1.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-6_1.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-7_1.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-8_1.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of mouse brain cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-9.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of mouse brain hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-ihc-testing-10.jpgAnti-NCBP1 Antibody Picoband® IHC analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in a paraffin-embedded section of rat brain hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-if-testing-11.jpgAnti-NCBP1 Antibody Picoband® IF analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). NCBP1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NCBP1 Antibody (A07424-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07424-3-ncbp1-primary-antibodies-fcm-testing-11.pngAnti-NCBP1 Antibody Picoband® Flow Cytometry analysis of JK cells using anti-NCBP1 antibody (A07424-3). Overlay histogram showing JK cells stained with A07424-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCBP1 Antibody (A07424-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-aadacl1-nceh1-picoband-trade-antibody-a06478-1-boster.html2026-03-17T05:14:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06478-1-nceh1-primary-antibodies-wb-testing-1.jpgAnti-AADACL1/NCEH1 Antibody Picoband® Western blot analysis of AADACL1/NCEH1 using anti-AADACL1/NCEH1 antibody (A06478-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AADACL1/NCEH1 antigen affinity purified polyclonal antibody (Catalog # A06478-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AADACL1/NCEH1 at approximately 46 kDa. The expected band size for AADACL1/NCEH1 is at 46,55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06478-1-nceh1-primary-antibodies-fcm-testing-2.pngAnti-AADACL1/NCEH1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-AADACL1/NCEH1 antibody (A06478-1). Overlay histogram showing HepG2 cells stained with A06478-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AADACL1/NCEH1 Antibody (A06478-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nr1-ndor1-picoband-trade-antibody-a09352-1-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09352-1-ndor1-primary-antibodies-wb-testing-1.jpgAnti-NR1/NDOR1 Antibody Picoband® Western blot analysis of NR1/NDOR1 using anti-NR1/NDOR1 antibody (A09352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1/NDOR1 antigen affinity purified polyclonal antibody (Catalog # A09352-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1/NDOR1 at approximately 72 kDa. The expected band size for NR1/NDOR1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09352-1-ndor1-primary-antibodies-if-testing-2.jpgAnti-NR1/NDOR1 Antibody Picoband® IF analysis of NR1/NDOR1 using anti-NR1/NDOR1 antibody (A09352-1). NR1/NDOR1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NR1/NDOR1 Antibody (A09352-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09352-1-ndor1-primary-antibodies-fcm-testing-3.pngAnti-NR1/NDOR1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-NR1/NDOR1 antibody (A09352-1). Overlay histogram showing MCF-7 cells stained with A09352-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR1/NDOR1 Antibody (A09352-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nebl-picoband-trade-antibody-a08817-2-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08817-2-nebl-primary-antibodies-wb-testing-1.jpgAnti-NEBL Antibody Picoband® Western blot analysis of NEBL using anti-NEBL antibody (A08817-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEBL antigen affinity purified polyclonal antibody (Catalog # A08817-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEBL at approximately 126 kDa. The expected band size for NEBL is at 116,126 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08817-2-nebl-primary-antibodies-ihc-testing-4.jpgAnti-NEBL Antibody Picoband®IHC analysis of NEBL using anti-NEBL antibody (A08817-2). NEBL was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEBL Antibody (A08817-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08817-2-nebl-primary-antibodies-ihc-testing-2.jpgAnti-NEBL Antibody Picoband® IHC analysis of NEBL using anti-NEBL antibody (A08817-2). NEBL was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEBL Antibody (A08817-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08817-2-nebl-primary-antibodies-ihc-testing-3.jpgAnti-NEBL Antibody Picoband® IHC analysis of NEBL using anti-NEBL antibody (A08817-2). NEBL was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEBL Antibody (A08817-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-necab1-picoband-trade-antibody-a13973-1-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-wb-testing-1_1.jpgAnti-NECAB1 Antibody Picoband®Western blot analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB1 antigen affinity purified polyclonal antibody (Catalog # A13973-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NECAB1 at approximately 41 kDa. The expected band size for NECAB1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-ihc-testing-1.jpgAnti-NECAB1 Antibody Picoband®IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). NECAB1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NECAB1 Antibody (A13973-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-ihc-testing-2_1.jpgAnti-NECAB1 Antibody Picoband®IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). NECAB1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NECAB1 Antibody (A13973-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-ihc-testing-3_1.jpgAnti-NECAB1 Antibody Picoband®IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). NECAB1 was detected in a paraffin-embedded section of mouse hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NECAB1 Antibody (A13973-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-ihc-testing-4_1.jpgAnti-NECAB1 Antibody Picoband®IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). NECAB1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NECAB1 Antibody (A13973-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-ihc-testing-5.jpgAnti-NECAB1 Antibody Picoband®IHC analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). NECAB1 was detected in a paraffin-embedded section of rat hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NECAB1 Antibody (A13973-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-if-testing-1.jpgAnti-NECAB1 Antibody Picoband®IF analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). NECAB1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NECAB1 Antibody (A13973-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13973-1-necab1-primary-antibodies-if-testing-2.jpgAnti-NECAB1 Antibody Picoband®IF analysis of NECAB1 using anti-NECAB1 antibody (A13973-1). NECAB1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NECAB1 Antibody (A13973-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-necab2-picoband-trade-antibody-a13886-2-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13886-2-necab2-primary-antibodies-wb-testing-1.jpgAnti-NECAB2 Antibody Picoband® Western blot analysis of NECAB2 using anti-NECAB2 antibody (A13886-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB2 antigen affinity purified polyclonal antibody (Catalog # A13886-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB2 at approximately 43 kDa. The expected band size for NECAB2 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13886-2-necab2-primary-antibodies-fcm-testing-2.pngAnti-NECAB2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-NECAB2 antibody (A13886-2). Overlay histogram showing MCF-7 cells stained with A13886-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NECAB2 Antibody (A13886-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-necab3-picoband-trade-antibody-a11525-3-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11525-3-necab3-primary-antibodies-wb-testing-1.jpgAnti-NECAB3 Antibody Picoband® Western blot analysis of NECAB3 using anti-NECAB3 antibody (A11525-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB3 antigen affinity purified polyclonal antibody (Catalog # A11525-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB3 at approximately 44 kDa. The expected band size for NECAB3 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11525-3-necab3-primary-antibodies-fcm-testing-2.pngAnti-NECAB3 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-NECAB3 antibody (A11525-3). Overlay histogram showing U251 cells stained with A11525-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NECAB3 Antibody (A11525-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sdccag1-nemf-picoband-trade-antibody-a09039-2-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09039-2-nemf-primary-antibodies-wb-testing-1.jpgAnti-SDCCAG1/NEMF Antibody Picoband® Western blot analysis of SDCCAG1/NEMF using anti-SDCCAG1/NEMF antibody (A09039-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDCCAG1/NEMF antigen affinity purified polyclonal antibody (Catalog # A09039-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDCCAG1/NEMF at approximately 140 kDa. The expected band size for SDCCAG1/NEMF is at 123,130-140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09039-2-nemf-primary-antibodies-if-testing-2.jpgAnti-SDCCAG1/NEMF Antibody Picoband® IF analysis of SDCCAG1/NEMF using anti-SDCCAG1/NEMF antibody (A09039-2) and anti-Beta Tubulin antibody (M01857-3). SDCCAG1/NEMF was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SDCCAG1/NEMF Antibody (A09039-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-neurl4-picoband-trade-antibody-a12035-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12035-neurl4-primary-antibodies-wb-testing-1.jpgAnti-NEURL4 Antibody Picoband® Western blot analysis of NEURL4 using anti-NEURL4 antibody (A12035). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEURL4 antigen affinity purified polyclonal antibody (Catalog # A12035) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEURL4 at approximately 185 kDa. The expected band size for NEURL4 is at 167 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12035-neurl4-primary-antibodies-ihc-testing-2.jpgAnti-NEURL4 Antibody Picoband® IHC analysis of NEURL4 using anti-NEURL4 antibody (A12035). NEURL4 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEURL4 Antibody (A12035) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12035-neurl4-primary-antibodies-fcm-testing-3.pngAnti-NEURL4 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-NEURL4 antibody (A12035). Overlay histogram showing U251 cells stained with A12035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEURL4 Antibody (A12035, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nfrkb-picoband-trade-antibody-a06867-2-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06867-2-nfrkb-primary-antibodies-wb-testing-1.jpgAnti-NFRKB Antibody Picoband® Western blot analysis of NFRKB using anti-NFRKB antibody (A06867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFRKB antigen affinity purified polyclonal antibody (Catalog # A06867-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFRKB at approximately 175 kDa. The expected band size for NFRKB is at 139 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06867-2-nfrkb-primary-antibodies-if-testing-2.jpgAnti-NFRKB Antibody Picoband® IF analysis of NFRKB using anti-NFRKB antibody (A06867-2). NFRKB was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NFRKB Antibody (A06867-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06867-2-nfrkb-primary-antibodies-fcm-testing-3.pngAnti-NFRKB Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-NFRKB antibody (A06867-2). Overlay histogram showing HEL cells stained with A06867-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFRKB Antibody (A06867-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nhlrc2-picoband-trade-antibody-a17232-1-boster.html2026-03-17T05:14:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17232-1-nhlrc2-primary-antibodies-wb-testing-1.jpgAnti-NHLRC2 Antibody Picoband® Western blot analysis of NHLRC2 using anti-NHLRC2 antibody (A17232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHLRC2 antigen affinity purified polyclonal antibody (Catalog # A17232-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHLRC2 at approximately 79 kDa. The expected band size for NHLRC2 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17232-1-nhlrc2-primary-antibodies-fcm-testing-2.pngAnti-NHLRC2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-NHLRC2 antibody (A17232-1). Overlay histogram showing U251 cells stained with A17232-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NHLRC2 Antibody (A17232-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nhsl2-picoband-trade-antibody-a18099-boster.html2026-03-17T05:14:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18099-nhsl2-primary-antibodies-wb-testing-1.jpgAnti-NHSL2 Antibody Picoband® Western blot analysis of NHSL2 using anti-NHSL2 antibody (A18099). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHSL2 antigen affinity purified polyclonal antibody (Catalog # A18099) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHSL2 at approximately 133 kDa. The expected band size for NHSL2 is at 133 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-nek6-picoband-trade-antibody-a03740-1-boster.html2026-03-17T05:14:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03740-1-nek6-primary-antibodies-wb-testing-1.jpgAnti-NEK6 Antibody Picoband® Western blot analysis of NEK6 using anti-NEK6 antibody (A03740-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HT-1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK6 antigen affinity purified polyclonal antibody (Catalog # A03740-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEK6 at approximately 45 kDa. The expected band size for NEK6 is at 36-45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03740-1-nek6-primary-antibodies-if-testing-2.jpgAnti-NEK6 Antibody Picoband® IF analysis of NEK6 using anti-NEK6 antibody (A03740-1). NEK6 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NEK6 Antibody (A03740-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03740-1-nek6-primary-antibodies-fcm-testing-3.pngAnti-NEK6 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-NEK6 antibody (A03740-1). Overlay histogram showing HepG2 cells stained with A03740-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEK6 Antibody (A03740-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-hoxd10-picoband-trade-antibody-a05257-2-boster.html2026-03-17T05:14:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05257-2-hoxd10-primary-antibodies-wb-testing-1.jpgAnti-HOXD10 Antibody Picoband® Western blot analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXD10 antigen affinity purified polyclonal antibody (Catalog # A05257-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXD10 at approximately 40 kDa. The expected band size for HOXD10 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05257-2-hoxd10-primary-antibodies-ihc-testing-2.jpgAnti-HOXD10 Antibody Picoband® IHC analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). HOXD10 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXD10 Antibody (A05257-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05257-2-hoxd10-primary-antibodies-ihc-testing-3.jpgAnti-HOXD10 Antibody Picoband® IHC analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). HOXD10 was detected in a paraffin-embedded section of human mucinous adenoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXD10 Antibody (A05257-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05257-2-hoxd10-primary-antibodies-ihc-testing-4.jpgAnti-HOXD10 Antibody Picoband® IHC analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). HOXD10 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXD10 Antibody (A05257-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05257-2-hoxd10-primary-antibodies-ihc-testing-5.jpgAnti-HOXD10 Antibody Picoband® IHC analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). HOXD10 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXD10 Antibody (A05257-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05257-2-hoxd10-primary-antibodies-ihc-testing-6.jpgAnti-HOXD10 Antibody Picoband® IHC analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). HOXD10 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXD10 Antibody (A05257-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05257-2-hoxd10-primary-antibodies-ihc-testing-7.jpgAnti-HOXD10 Antibody Picoband® IHC analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). HOXD10 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXD10 Antibody (A05257-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-aldh1a2-picoband-trade-antibody-a04961-2-boster.html2026-03-17T05:14:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04961-2-aldh1a2-primary-antibodies-wb-testing-1.jpgAnti-ALDH1A2 Antibody Picoband® Western blot analysis of ALDH1A2 using anti-ALDH1A2 antibody (A04961-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A2 antigen affinity purified polyclonal antibody (Catalog # A04961-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A2 at approximately 57 kDa. The expected band size for ALDH1A2 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-chek2-picoband-trade-antibody-a00277-2-boster.html2026-03-17T05:14:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00277-2-chek2-primary-antibodies-wb-testing-1.jpgAnti-CHEK2 Antibody Picoband® Western blot analysis of CHEK2 using anti-CHEK2 antibody (A00277-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 antigen affinity purified polyclonal antibody (Catalog # A00277-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK2 at approximately 62 kDa. The expected band size for CHEK2 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00277-2-chek2-primary-antibodies-if-testing-2.jpgAnti-CHEK2 Antibody Picoband® IF analysis of CHEK2 using anti-CHEK2 antibody (A00277-2) and anti-Beta Tubulin antibody (M01857-3). CHEK2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CHEK2 Antibody (A00277-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-cyc1-picoband-trade-antibody-a02958-2-boster.html2026-03-17T05:14:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02958-2-cyc1-primary-antibodies-wb-testing-1.jpgAnti-CYC1 Antibody Picoband® Western blot analysis of CYC1 using anti-CYC1 antibody (A02958-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYC1 antigen affinity purified polyclonal antibody (Catalog # A02958-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYC1 at approximately 33 kDa. The expected band size for CYC1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02958-2-cyc1-primary-antibodies-if-testing-2.jpgAnti-CYC1 Antibody Picoband® IF analysis of CYC1 using anti-CYC1 antibody (A02958-2). CYC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CYC1 Antibody (A02958-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02958-2-cyc1-primary-antibodies-fcm-testing-3.pngAnti-CYC1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-CYC1 antibody (A02958-2). Overlay histogram showing MCF-7 cells stained with A02958-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYC1 Antibody (A02958-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-sox2-picoband-trade-antibody-a00105-1-boster.html2026-03-17T05:14:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00105-1-sox2-primary-antibodies-wb-testing-1.jpgAnti-SOX2 Antibody Picoband® Western blot analysis of SOX2 using anti-SOX2 antibody (A00105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 ma for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 antigen affinity purified poly clonal antibody (Catalog # A00105-1) at 0.5 μg/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit ING-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Luminescence detection (ECL) kit (Catalog # EK1002) with Anon 5200 system. A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-sam68-khdrbs1-picoband-trade-antibody-a01717-1-boster.html2026-03-17T05:14:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-wb-testing-1.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® Western blot analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sam68/KHDRBS1 antigen affinity purified polyclonal antibody (Catalog # A01717-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sam68/KHDRBS1 at approximately 68 kDa. The expected band size for Sam68/KHDRBS1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-2.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-3.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-4.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-5.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of human keratinizing squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-6.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of human ovarian adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-7.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of human papillary thyroid carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-8.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-ihc-testing-9.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IHC analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-if-testing-10.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IF analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1) and anti-Beta Tubulin antibody (M01857-3). Sam68/KHDRBS1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-if-testing-11.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IF analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-if-testing-12.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IF analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-if-testing-13.jpgAnti-Sam68/KHDRBS1 Antibody Picoband® IF analysis of Sam68/KHDRBS1 using anti-Sam68/KHDRBS1 antibody (A01717-1). Sam68/KHDRBS1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-fcm-testing-14.pngAnti-Sam68/KHDRBS1 Antibody Picoband® Flow Cytometry analysis of HEPA1-6 cells using anti-Sam68/KHDRBS1 antibody (A01717-1). Overlay histogram showing HEPA1-6 cells stained with A01717-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01717-1-khdrbs1-primary-antibodies-fcm-testing-15.pngAnti-Sam68/KHDRBS1 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-Sam68/KHDRBS1 antibody (A01717-1). Overlay histogram showing Raji cells stained with A01717-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sam68/KHDRBS1 Antibody (A01717-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/anti-nelfe-picoband-trade-antibody-a07050-1-boster.html2026-03-17T05:14:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07050-1-nelfe-primary-antibodies-wb-testing-1.jpgAnti-NELFE Antibody Picoband® Western blot analysis of NELFE using anti-NELFE antibody (A07050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NELFE antigen affinity purified polyclonal antibody (Catalog # A07050-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NELFE at approximately 43 kDa. The expected band size for NELFE is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07050-1-nelfe-primary-antibodies-ihc-testing-2.jpgAnti-NELFE Antibody Picoband® IHC analysis of NELFE using anti-NELFE antibody (A07050-1). NELFE was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NELFE Antibody (A07050-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07050-1-nelfe-primary-antibodies-ihc-testing-3.jpgAnti-NELFE Antibody Picoband® IHC analysis of NELFE using anti-NELFE antibody (A07050-1). NELFE was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NELFE Antibody (A07050-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07050-1-nelfe-primary-antibodies-if-testing-4.jpgAnti-NELFE Antibody Picoband® IF analysis of NELFE using anti-NELFE antibody (A07050-1) and anti-Beta Tubulin antibody (M01857-3). NELFE was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NELFE Antibody (A07050-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07050-1-nelfe-primary-antibodies-fcm-testing-5.pngAnti-NELFE Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-NELFE antibody (A07050-1). Overlay histogram showing 293T cells stained with A07050-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NELFE Antibody (A07050-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.